LOCUS       DAFBYI010000001        72551 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFBYI010000001 DAFBYI010000000
VERSION     DAFBYI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.001"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..422
                     /locus_tag="LLD04_000001"
     CDS             <1..422
                     /locus_tag="LLD04_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK5646968.1"
                     /translation="TLIDWLKARSRPFLFSTSLTPGAAAAALASITLMQEHPELVEKV
                     WENANYFKEELKKVGYNIGMSETPITPVILGDEKVTQTFSKKLIEHGIYAKPIVYPTV
                     PLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKEMEII"
     gene            524..1477
                     /locus_tag="LLD04_000002"
     CDS             524..1477
                     /locus_tag="LLD04_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK5646969.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            1546..2895
                     /locus_tag="LLD04_000003"
     CDS             1546..2895
                     /locus_tag="LLD04_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5646970.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(3078..3401)
                     /locus_tag="LLD04_000004"
     CDS             complement(3078..3401)
                     /locus_tag="LLD04_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5646971.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            3530..4693
                     /locus_tag="LLD04_000005"
     CDS             3530..4693
                     /locus_tag="LLD04_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5646972.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            4763..6112
                     /locus_tag="LLD04_000006"
     CDS             4763..6112
                     /locus_tag="LLD04_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5646973.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6157..6588)
                     /locus_tag="LLD04_000007"
     CDS             complement(6157..6588)
                     /locus_tag="LLD04_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5646974.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            6976..9618
                     /gene="alaS"
                     /locus_tag="LLD04_000008"
     CDS             6976..9618
                     /gene="alaS"
                     /locus_tag="LLD04_000008"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK5646975.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            9818..10414
                     /locus_tag="LLD04_000009"
     CDS             9818..10414
                     /locus_tag="LLD04_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5646976.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            10536..11684
                     /locus_tag="LLD04_000010"
     CDS             10536..11684
                     /locus_tag="LLD04_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5646977.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SSPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(11722..12135)
                     /locus_tag="LLD04_000011"
     CDS             complement(11722..12135)
                     /locus_tag="LLD04_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK5646978.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            12214..12645
                     /locus_tag="LLD04_000012"
     CDS             12214..12645
                     /locus_tag="LLD04_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK5646979.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(12744..12977)
                     /locus_tag="LLD04_000013"
     CDS             complement(12744..12977)
                     /locus_tag="LLD04_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK5646980.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            13160..14830
                     /locus_tag="LLD04_000014"
     CDS             13160..14830
                     /locus_tag="LLD04_000014"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK5646981.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            14977..15363
                     /locus_tag="LLD04_000015"
     CDS             14977..15363
                     /locus_tag="LLD04_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5646982.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            15350..16048
                     /locus_tag="LLD04_000016"
     CDS             15350..16048
                     /locus_tag="LLD04_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5646983.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            16053..16517
                     /locus_tag="LLD04_000017"
     CDS             16053..16517
                     /locus_tag="LLD04_000017"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK5646984.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            16514..17425
                     /locus_tag="LLD04_000018"
     CDS             16514..17425
                     /locus_tag="LLD04_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5646985.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            17660..18199
                     /gene="pyrR"
                     /locus_tag="LLD04_000019"
     CDS             17660..18199
                     /gene="pyrR"
                     /locus_tag="LLD04_000019"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK5646986.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            18213..19496
                     /locus_tag="LLD04_000020"
     CDS             18213..19496
                     /locus_tag="LLD04_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK5646987.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            19509..20435
                     /locus_tag="LLD04_000021"
     CDS             19509..20435
                     /locus_tag="LLD04_000021"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK5646988.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            20454..21734
                     /locus_tag="LLD04_000022"
     CDS             20454..21734
                     /locus_tag="LLD04_000022"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK5646989.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQRFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            21739..22815
                     /gene="carA"
                     /locus_tag="LLD04_000023"
     CDS             21739..22815
                     /gene="carA"
                     /locus_tag="LLD04_000023"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK5646990.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            22820..26005
                     /gene="carB"
                     /locus_tag="LLD04_000024"
     CDS             22820..26005
                     /gene="carB"
                     /locus_tag="LLD04_000024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK5646991.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            26059..26835
                     /locus_tag="LLD04_000025"
     CDS             26059..26835
                     /locus_tag="LLD04_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK5646992.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            26832..27764
                     /locus_tag="LLD04_000026"
     CDS             26832..27764
                     /locus_tag="LLD04_000026"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK5646993.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            27761..28477
                     /gene="pyrF"
                     /locus_tag="LLD04_000027"
     CDS             27761..28477
                     /gene="pyrF"
                     /locus_tag="LLD04_000027"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK5646994.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            28484..29113
                     /locus_tag="LLD04_000028"
     CDS             28484..29113
                     /locus_tag="LLD04_000028"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK5646995.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            29101..29415
                     /locus_tag="LLD04_000029"
     CDS             29101..29415
                     /locus_tag="LLD04_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5646996.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            29453..30094
                     /locus_tag="LLD04_000030"
     CDS             29453..30094
                     /locus_tag="LLD04_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5646997.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            30137..30829
                     /locus_tag="LLD04_000031"
     CDS             30137..30829
                     /locus_tag="LLD04_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK5646998.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            30826..31728
                     /locus_tag="LLD04_000032"
     CDS             30826..31728
                     /locus_tag="LLD04_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5646999.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(31886..33592)
                     /gene="efbA"
                     /locus_tag="LLD04_000033"
     CDS             complement(31886..33592)
                     /gene="efbA"
                     /locus_tag="LLD04_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK5647000.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            33782..34405
                     /locus_tag="LLD04_000034"
     CDS             33782..34405
                     /locus_tag="LLD04_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647001.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            34854..35903
                     /locus_tag="LLD04_000035"
     CDS             34854..35903
                     /locus_tag="LLD04_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5647002.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKKTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            35900..36781
                     /locus_tag="LLD04_000036"
     CDS             35900..36781
                     /locus_tag="LLD04_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647003.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            36784..37554
                     /locus_tag="LLD04_000037"
     CDS             36784..37554
                     /locus_tag="LLD04_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647004.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            37833..38687
                     /gene="aroE"
                     /locus_tag="LLD04_000038"
     CDS             37833..38687
                     /gene="aroE"
                     /locus_tag="LLD04_000038"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK5647005.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            38865..40028
                     /locus_tag="LLD04_000039"
     CDS             38865..40028
                     /locus_tag="LLD04_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5647006.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            40145..40555
                     /locus_tag="LLD04_000040"
     CDS             40145..40555
                     /locus_tag="LLD04_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5647007.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            40729..42957
                     /gene="topB"
                     /locus_tag="LLD04_000041"
     CDS             40729..42957
                     /gene="topB"
                     /locus_tag="LLD04_000041"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5647008.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            43128..43412
                     /locus_tag="LLD04_000042"
     CDS             43128..43412
                     /locus_tag="LLD04_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647009.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            43572..43808
                     /locus_tag="LLD04_000043"
     CDS             43572..43808
                     /locus_tag="LLD04_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5647010.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            43805..44203
                     /locus_tag="LLD04_000044"
     CDS             43805..44203
                     /locus_tag="LLD04_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK5647011.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(44870..45814)
                     /locus_tag="LLD04_000045"
     CDS             complement(44870..45814)
                     /locus_tag="LLD04_000045"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5647012.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(46110..47054)
                     /locus_tag="LLD04_000046"
     CDS             complement(46110..47054)
                     /locus_tag="LLD04_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647013.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            47275..48489
                     /locus_tag="LLD04_000047"
     CDS             47275..48489
                     /locus_tag="LLD04_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5647014.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            48511..50526
                     /locus_tag="LLD04_000048"
     CDS             48511..50526
                     /locus_tag="LLD04_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK5647015.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(50609..51982)
                     /locus_tag="LLD04_000049"
     CDS             complement(50609..51982)
                     /locus_tag="LLD04_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5647016.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(52008..52292)
                     /locus_tag="LLD04_000050"
     CDS             complement(52008..52292)
                     /locus_tag="LLD04_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647017.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(52294..54309)
                     /locus_tag="LLD04_000051"
     CDS             complement(52294..54309)
                     /locus_tag="LLD04_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5647018.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(54488..54793)
                     /locus_tag="LLD04_000052"
     CDS             complement(54488..54793)
                     /locus_tag="LLD04_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK5647019.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(54882..55913)
                     /locus_tag="LLD04_000053"
     CDS             complement(54882..55913)
                     /locus_tag="LLD04_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5647020.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            56120..56995
                     /locus_tag="LLD04_000054"
     CDS             56120..56995
                     /locus_tag="LLD04_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5647021.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(57147..57989)
                     /locus_tag="LLD04_000055"
     CDS             complement(57147..57989)
                     /locus_tag="LLD04_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5647022.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(58081..59958)
                     /locus_tag="LLD04_000056"
     CDS             complement(58081..59958)
                     /locus_tag="LLD04_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5647023.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(59955..61376)
                     /locus_tag="LLD04_000057"
     CDS             complement(59955..61376)
                     /locus_tag="LLD04_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5647024.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(61379..64768)
                     /locus_tag="LLD04_000058"
     CDS             complement(61379..64768)
                     /locus_tag="LLD04_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5647025.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPEYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            65025..66416
                     /locus_tag="LLD04_000059"
     CDS             65025..66416
                     /locus_tag="LLD04_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647026.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(66489..67136)
                     /locus_tag="LLD04_000060"
     CDS             complement(66489..67136)
                     /locus_tag="LLD04_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5647027.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(67144..67830)
                     /locus_tag="LLD04_000061"
     CDS             complement(67144..67830)
                     /locus_tag="LLD04_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647028.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(67836..68909)
                     /locus_tag="LLD04_000062"
     CDS             complement(67836..68909)
                     /locus_tag="LLD04_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647029.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            69131..69805
                     /locus_tag="LLD04_000063"
     CDS             69131..69805
                     /locus_tag="LLD04_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5647030.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            69810..71150
                     /locus_tag="LLD04_000064"
     CDS             69810..71150
                     /locus_tag="LLD04_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5647031.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(71299..72285)
                     /locus_tag="LLD04_000065"
     CDS             complement(71299..72285)
                     /locus_tag="LLD04_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK5647032.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        22759 a        13009 c        14720 g        22063 t
ORIGIN      
        1 aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct acttcgctaa
       61 ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa catccggaac
      121 ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa aaagtaggct
      181 ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac gaaaaagtga
      241 ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca atcgtttatc
      301 caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa catacgaggg
      361 aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg gaaattattt
      421 aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc atatattgtt
      481 aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa aagtattggt
      541 gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc aagaactagg
      601 cagtgaaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga aaaacgatat
      661 ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag aacaatatca
      721 agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag caaaaccaca
      781 attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg ctagagaatt
      841 ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata ctccaaaaga
      901 tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc
      961 tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc gtggtgtccg
     1021 tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag actacgcagt
     1081 cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg atcaaggtac
     1141 atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac tcatgaatgc
     1201 agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt cttttgaccc
     1261 ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg attatgatgt
     1321 ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg tttcttgtgc
     1381 gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa aggatatgtt
     1441 ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca tccaaaaagt
     1501 tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga agaatatcaa
     1561 gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat gggatttttt
     1621 actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt taaagccgca
     1681 agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt tctcggtggt
     1741 atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat tccagaaaca
     1801 ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt tttattgggc
     1861 tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat cattttcggt
     1921 acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt agctgttaca
     1981 gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg ggcttttcag
     2041 tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt cggtttgttc
     2101 cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa tctttcattt
     2161 ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg ttggatacac
     2221 gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa agcaatttct
     2281 ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt cttaagaacc
     2341 gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt
     2401 tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt ttatggaacc
     2461 atcaatggct atacccttac tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac
     2521 ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc tgtagccgca
     2581 ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt tgatacacta
     2641 acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt tggtgtcatt
     2701 cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat gtatccgttt
     2761 attccactag tagcaatcat tggagggact tttatcgtat caagcacgct tatcactcag
     2821 acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat ttatctctat
     2881 ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct ctgccaaaca
     2941 aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc gttaattttg
     3001 atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag aaatcgggct
     3061 caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg tacgccgcga
     3121 taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat cagatagaca
     3181 gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc aagaagtgct
     3241 tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag gagaataaat
     3301 cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata aaggaacagt
     3361 aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc ccctttgtgt
     3421 ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg cttgaaacag
     3481 caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata tggcaagtta
     3541 tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag cgatctcatc
     3601 tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa gggcagttat
     3661 tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct taccacttcc
     3721 ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt tatttgcttc
     3781 atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta cctacctgcc
     3841 ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac tcataccatt
     3901 tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga ttgctagttt
     3961 ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta tctacccaag
     4021 accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg gaatcattgg
     4081 ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa tggatgaact
     4141 ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt atttgatcga
     4201 ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga
     4261 ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg tatactttat
     4321 gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc gtatccgttt
     4381 ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct acgggtatct
     4441 gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa tcatgaattc
     4501 gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg tcttgttact
     4561 acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac ggattctgtt
     4621 ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa aggatgatga
     4681 ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa
     4741 tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca aaccgtttat
     4801 caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg aacgtttgat
     4861 tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta caggaaaaac
     4921 acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt
     4981 tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag ctacacaaat
     5041 cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta cagacaaaca
     5101 gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca cgcctggtag
     5161 aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg catttgttgt
     5221 cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc aaattgcaag
     5281 tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag aaaaacttcg
     5341 tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt
     5401 tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg agaatcaaat
     5461 gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg ccaatacaaa
     5521 acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat
     5581 tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac agaatctgga
     5641 gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg aaggtgtctc
     5701 tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc gtgtcggacg
     5761 tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa atgatgagca
     5821 agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg
     5881 ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt
     5941 attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta
     6001 taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca aaatcgaacg
     6061 cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag
     6121 aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt ttttcattcc
     6181 gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc cttgtttttc
     6241 ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac cttctgaaac
     6301 cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc ggtattcttt
     6361 tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt
     6421 agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga tatcttttcc
     6481 gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat atctcaaaaa
     6541 gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca tattcctcct
     6601 tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa aatcgtggag
     6661 tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa tagaatgcgt
     6721 atactaaata agtacgaaaa ggatatgatt tgttttttaa gactcacaga aaattctttc
     6781 ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt tggtagaaat
     6841 accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa
     6901 ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt taatttatag
     6961 aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat gtttcttgat
     7021 ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat
     7081 ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt tgatggttca
     7141 gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac aaatgatatc
     7201 gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg taatttttcg
     7261 attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt gactgatcca
     7321 aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa agatacagaa
     7381 gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat tgatgtagaa
     7441 gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat tttctatgac
     7501 cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc aggaggagaa
     7561 aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca taagcctgat
     7621 aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt ggaacgcatg
     7681 gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat gcctattatc
     7741 cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt aacagatatt
     7801 tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg cgacggtgca
     7861 ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg
     7921 catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc tgttgttggg
     7981 gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt
     8041 atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga aatcttaaat
     8101 caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa agacatcttc
     8161 aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc agaagatgaa
     8221 ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct
     8281 cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt gacagatatc
     8341 aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca attattggtc
     8401 atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt
     8461 gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg atggatcatg
     8521 aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc taatggtcaa
     8581 tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta tcatctattg
     8641 atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca tctactgcac
     8701 aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt agtcgctcct
     8761 ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga attagctgaa
     8821 atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac aattgaaaca
     8881 gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa atacggacat
     8941 gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg cacacatgta
     9001 gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc
     9061 gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga agaagaagaa
     9121 aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga agttgtttca
     9181 aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga acaattagct
     9241 agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac gatcaatgga
     9301 aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg tcaattagct
     9361 gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga acaagaagga
     9421 aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact aaaagctggc
     9481 gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg tccagacatg
     9541 gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc tgcgaaagaa
     9601 tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa
     9661 cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt tttcaattgg
     9721 atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa agtgacagaa
     9781 atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa ataagacctt
     9841 tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga tggttccttg
     9901 gctggggttt attccattag gattcattag tttaacaatt atccatatca cagtaattat
     9961 tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt ggggaatcgc
    10021 tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag tgttcacgaa
    10081 tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct tggttttcca
    10141 ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag gggtactggg
    10201 ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata caggtgcaac
    10261 ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag gggttgcagc
    10321 aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac tagcaaaagc
    10381 gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata gtaataggac
    10441 gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc catcttttta
    10501 cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa
    10561 agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc ttaggattgg
    10621 ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca ggaagtgtct
    10681 ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata ttgactttga
    10741 tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga cacgaacgct
    10801 tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga tttcaatttt
    10861 tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca cccatcgtct
    10921 tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg tatcaggtta
    10981 ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt tataatgatg
    11041 tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact ggatggcgta
    11101 ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga ttgacgatgc
    11161 ttcgaaattt tgtatacgaa ctattaggaa gtagcccaac gccagcagag attgaagaaa
    11221 tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta ttggtgcata
    11281 actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa tcaatgaatt
    11341 tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag tctttggatg
    11401 tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac tataatatct
    11461 tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt catgatttta
    11521 gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag aaattccagt
    11581 ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa atcggagatt
    11641 accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga aactataaaa
    11701 agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt tatggagagc
    11761 ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa
    11821 taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga agagctcgtt
    11881 cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat
    11941 cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa agacttcgaa
    12001 ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt gtttaccttg
    12061 atagacaatc aagattccac ctccactagg accagggttg ccttttgtag aggcatcaat
    12121 gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt atggtaagat
    12181 tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg atggcagcct
    12241 gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt ttttagtctg
    12301 attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt aggtattttt
    12361 cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa ctctgtgatt
    12421 atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat tcgatctttg
    12481 acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc atctgttttc
    12541 atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa cggacccaaa
    12601 tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta aaaatcagaa
    12661 cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt catcccgttt
    12721 agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt ctgaacgttg
    12781 cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc aaattcgacc
    12841 acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa atgtacaaaa
    12901 atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc aaaccatttt
    12961 acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag ccttcattat
    13021 aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg ttttttttct
    13081 gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt gaataaagtt
    13141 cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa cagagctaga
    13201 accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg aactgtatgg
    13261 gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata acgctgaggg
    13321 gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa agtcaacgat
    13381 cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta ttgcattgcg
    13441 agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg gaggatatgc
    13501 acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc atgctatcac
    13561 aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag gaaatgaatt
    13621 gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg atcgtgaact
    13681 gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag aagatgggtt
    13741 tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag accttgaaga
    13801 tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc agcctgtgac
    13861 tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg caatcaagcc
    13921 gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac cgtttgccaa
    13981 tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat tagcagatta
    14041 cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt tagatatcaa
    14101 agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg tccgtgcatt
    14161 gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg atgctttgaa
    14221 agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc agctacctgt
    14281 gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac tgttggagca
    14341 cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag atggagcaga
    14401 aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag aagctaatta
    14461 caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa tcgtcaagaa
    14521 aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa tagaagagtt
    14581 tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt attcattttc
    14641 tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc gcgaaatcat
    14701 cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga cgatgcctgg
    14761 tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag aagtgttggg
    14821 attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac tacagcttct
    14881 ttttttagac gatagggaat taattttgtt tacactagga tcattctttt tctttattgt
    14941 attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt tataagaaaa
    15001 accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga aaaacgcagt
    15061 gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac gatgcaggtc
    15121 accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc tgcttatgtg
    15181 atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc aactgggttg
    15241 ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt gttgattgcg
    15301 ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta tggcaaactc
    15361 tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg agatgggaaa
    15421 tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa aacaattaat
    15481 gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg cgatcgtcca
    15541 tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag tcaagcgaat
    15601 ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg
    15661 tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag tagcagaaaa
    15721 aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga ttactttacg
    15781 taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt taacaaataa
    15841 acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt ttgttccagc
    15901 taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt tggaagccgt
    15961 acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac gacctcgtga
    16021 tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg atattcagcg
    16081 ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat ctagcgcttg
    16141 gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga aacactggag
    16201 ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca ctcattgcag
    16261 tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga
    16321 ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt attcgtcttg
    16381 gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt aacgtagcag
    16441 atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta gatgaaaagg
    16501 atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg gtaggatcga
    16561 taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc tgtggctgaa
    16621 aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag taaaacaagg
    16681 agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc cagaaaatat
    16741 cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat
    16801 ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca
    16861 tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc accgaatcga
    16921 caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg aatcattagc
    16981 tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc atggagagat
    17041 tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat
    17101 gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat tggaacgttt
    17161 cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc agatccgtgt
    17221 gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac cgaagaaaac
    17281 tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc acccgactac
    17341 aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa
    17401 attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac aacgaaagca
    17461 taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag
    17521 gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta tcagtgtatc
    17581 agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt ttttatgcaa
    17641 aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg
    17701 agcattgact cgaattactt atgagattat cgagagaaac catagtatcc aagatatcgt
    17761 attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg aacgcctgaa
    17821 gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat accgagatga
    17881 caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag tttccttaga
    17941 aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga ttcgagcagc
    18001 aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag tcctggtcga
    18061 tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca ttccaacatc
    18121 tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc gcatcatgat
    18181 tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga tttcataatg
    18241 atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta ggattaagtt
    18301 tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg
    18361 atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta tttactacta
    18421 aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg atgcagcttt
    18481 tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact ggattagtct
    18541 atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat aaaatcttgc
    18601 ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag
    18661 caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct gtagcgctga
    18721 tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc ggattgattc
    18781 cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga atcgtggata
    18841 ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt ccctttgtac
    18901 aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc gcttttgtga
    18961 cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa cgtaatttct
    19021 ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa atcgtggcag
    19081 cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt ctggctatca
    19141 cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc attttaggat
    19201 tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc tcaggaatca
    19261 gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc gagaacaaaa
    19321 tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta gtaggaattg
    19381 gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta gcaactgtac
    19441 ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa aactaaggag
    19501 gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg accgttgagg
    19561 cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc aagcaaggtg
    19621 ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc gaaaatagta
    19681 ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa gtgattgaat
    19741 ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca gtactgacga
    19801 tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac tattatgacg
    19861 aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac ggaagcggac
    19921 agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt ggtcattttg
    19981 aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct aaatccaata
    20041 tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga gaatggtacg
    20101 acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta gatcaagttg
    20161 atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa agtttctcaa
    20221 aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac
    20281 atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct gattcattag
    20341 tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc gtcagaatgg
    20401 caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa
    20461 cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca gcagaaattt
    20521 ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg
    20581 aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc catgttcatt
    20641 tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc tcagctgctc
    20701 gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag
    20761 aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag gtattgcagt
    20821 atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa gcactgattg
    20881 aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt
    20941 atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat acagaagatg
    21001 aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc
    21061 ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg ctagctgaag
    21121 caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc cgtgtaatca
    21181 gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat catcttattt
    21241 tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg ccgctaagag
    21301 gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc gattgcatcg
    21361 ctacagacca tgccccccat ggattagaag aaaaaagcca aaggttcatg aaatcaccat
    21421 ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt gtagaaactg
    21481 gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct gagatttttg
    21541 gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt tttgatatta
    21601 cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat acacctttta
    21661 tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc
    21721 agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac agttttcgaa
    21781 ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg
    21841 actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat tacatttacc
    21901 tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat ttctccaaca
    21961 tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg aaaccaaatg
    22021 acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat cgatacgcgc
    22081 gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt tgatgctgga
    22141 gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac caatcaagtg
    22201 cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa agtcattgtc
    22261 attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa ctgtaacctc
    22321 acagtacttc cctataacac agatgccgag acgattttag cgctatcacc agacggagtc
    22381 atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa
    22441 aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt atttgcttta
    22501 gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa tcacccggta
    22561 cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta tgcagtcgat
    22621 gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa tgacggaaca
    22681 gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca tccagatgct
    22741 gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat gatggacgca
    22801 ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta tggtgattgg
    22861 atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa cacaagcctg
    22921 ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc ctgcgacgat
    22981 catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac ttgaatttgt
    23041 atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag gcggacagac
    23101 aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat tgaacataga
    23161 acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca
    23221 gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata cagtagaaga
    23281 agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag cctttacttt
    23341 aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa
    23401 tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg caggttttaa
    23461 agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg tctgcaatat
    23521 ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg cccctagtca
    23581 aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga ttattcgagc
    23641 acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata gttttaatta
    23701 ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac
    23761 tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat tagacgaaat
    23821 gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag attatgttgt
    23881 agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc ttttaggcac
    23941 gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag agtctttatt
    24001 gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg agttggcaga
    24061 catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc gcctgtttta
    24121 tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt tgacaaaaat
    24181 cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc agttgaaaca
    24241 gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta cagacaaaaa
    24301 agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa aagaaaatga
    24361 tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat caaaaacacc
    24421 ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa agccctcaat
    24481 tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg actatgcgac
    24541 tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca tgaacagcaa
    24601 tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg agccattgac
    24661 cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa tcgttcaatt
    24721 tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg taaaaatctt
    24781 agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct
    24841 gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag aagaagccgt
    24901 ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg tattaggcgg
    24961 acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg aacatgctgt
    25021 gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg gtgaatgcga
    25081 agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg agcatattga
    25141 gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa cattatctga
    25201 atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt tgaattgtgt
    25261 cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa
    25321 tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc caatggcaca
    25381 aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg aaaacggact
    25441 ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta cgaaattgca
    25501 gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa tgggatctga
    25561 cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac acttgcctga
    25621 atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac tagaattagc
    25681 taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag ctgcttattt
    25741 cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa
    25801 aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata cgattgataa
    25861 agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag ttgaacatgg
    25921 tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga tggaatcccg
    25981 tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac
    26041 cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca aaaacagctg
    26101 gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat gcagcaacca
    26161 ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg accgattagc
    26221 ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt ggaaggtgag
    26281 gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat gggtccttta
    26341 ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat aatcggtgga
    26401 ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa aggaattaag
    26461 ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa agaatttaat
    26521 cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca cggaaatgtc
    26581 ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg tggttcaaac
    26641 gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca gttatcatta
    26701 gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca tacgccggaa
    26761 gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga
    26821 gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta gaattaaaaa
    26881 atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga aaatactatg
    26941 atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc cgctttggca
    27001 atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa
    27061 atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag tacaatgagt
    27121 tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca
    27181 aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca
    27241 aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta acacaagcag
    27301 taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta acagacatcg
    27361 ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg atcaatactc
    27421 ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat caaacaggtg
    27481 gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa gtagcaagta
    27541 tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat gttcttgaga
    27601 tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc gatccgtata
    27661 tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc atcgaatcgc
    27721 tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac tagaccaacg
    27781 accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc ttcgattttt
    27841 cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc
    27901 tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc tgaaacttca
    27961 cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg gtgttgatct
    28021 cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc gaggattaga
    28081 agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc agcttacttc
    28141 tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag aaaaaagtgt
    28201 gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt cttctgcttg
    28261 ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa ctccgggtat
    28321 aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt ctcaggcaaa
    28381 aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa ctaatcctta
    28441 taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg ttgaacaaag
    28501 tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta atccttttac
    28561 atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga tgagctatcc
    28621 aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag cttaccctga
    28681 agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat gggtagcgga
    28741 aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa
    28801 ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg atttgatttc
    28861 tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg cggatatcct
    28921 tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc
    28981 cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag ctttagaaga
    29041 tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag atcctgaaaa
    29101 ttggcagatt taagcgcgct tgatgaagat agagaaatcc gatttgtgtc tccagaatca
    29161 tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc atcaggtatg
    29221 aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt tctaagactg
    29281 gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag attttctcta
    29341 ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt
    29401 tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt
    29461 gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac ctggattttt
    29521 taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt
    29581 tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc tctatgaaaa
    29641 atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc cggtgaaata
    29701 cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga cggacgaatt
    29761 catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt tgttcgaagg
    29821 aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg accttcttta
    29881 tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg agatgttcat
    29941 tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt atttctttaa
    30001 agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt ttttagaaac
    30061 cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc agggggaagt
    30121 atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag gtccggaaaa
    30181 ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc agtcgcctat
    30241 tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct ttcattatca
    30301 aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac cttttgacca
    30361 actcagttat gtagagttca agaaagaacg ctattatttg acagatattc atttccactt
    30421 gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt ttgttcatat
    30481 gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg tacaggaagg
    30541 ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg agcattgggt
    30601 aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg gctcattgac
    30661 aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag gaaaactgaa
    30721 ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc gaccaatcca
    30781 gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa tattcagcag
    30841 atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc aaagaaaatg
    30901 tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga gttagggatc
    30961 acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca agacttctta
    31021 aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta tctaacgagt
    31081 gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg tcctttgacc
    31141 gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat ggagacgaca
    31201 acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat tatttatatg
    31261 aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct aagtggccgc
    31321 agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt agctgaacac
    31381 gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac acaggatgga
    31441 aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga aacagtgatt
    31501 tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc ttacgcttca
    31561 ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc tttggcagaa
    31621 ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag tgaaatcgct
    31681 caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga aaagcagatg
    31741 agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag aattttgtca
    31801 caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga aagtacgcaa
    31861 atatgattct agaaacactc attgattacc tgttttgttt catagctgta attttttctt
    31921 cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca ggcttggcgc
    31981 catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg gcagagtatc
    32041 ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca tctgagggct
    32101 tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg
    32161 tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta ccaacaagaa
    32221 tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta cgtttttgtt
    32281 tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta attgcttcga
    32341 tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat tggatttcat
    32401 ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc ttttggtatc
    32461 gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt gctattttga
    32521 ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg cgtggtactt
    32581 gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg ttttcggatt
    32641 ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt tcattttcga
    32701 tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat
    32761 aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat gcctgttcgg
    32821 acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag
    32881 gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc ttttcattag
    32941 gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt ccctgaaact
    33001 gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag attttttctt
    33061 tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat tctacaccag
    33121 gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca tccagtattt
    33181 ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat tcaacgatca
    33241 atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa tggatcactc
    33301 gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat tttcgaagca
    33361 tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta atctgtacac
    33421 gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt gaacgaatca
    33481 ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca gatagtaacg
    33541 tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac atggttcatt
    33601 cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa accaatttgg
    33661 ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa aacagtgttt
    33721 ctatcgttga aatccaaatt tttaggtata ataaaaaata ggagaaaaag tagaaaagaa
    33781 ggtgtttgtt atttgggaag tagttaaatc gagagtacaa gaaatgaatg aacatttctc
    33841 agtgtcattc gcagaaaatc caagtaagta ttttcgacgt cttgtttttc tgctcatctt
    33901 atcattgctg ttaacagttg caatgttttt attgccggaa aaaatccgag aagagcaagt
    33961 aaatagtctc gtttattcgt ccataccaac cagcaaattg aaaccttttg attacagcaa
    34021 agctgatcag aaaatcaaac aaagccgggc gatatcggtg atgttcagca aaccgaacgg
    34081 gaaaacctat caggacatgc ttgatgtttt taatgatcct gagcttatgg aagaattgaa
    34141 tcgaccgatc ttttattatc cgatcgttta tgatgtacat gaactggaac aaaaatataa
    34201 catacgaacg gatgaagtga catttatttt ctttgatgga ggaaaagaag caaatcggat
    34261 aactgcgggt aagggaggaa taactgattt tgataaggag ttgatccctg aactgaatcg
    34321 acttccgtta gccaatatca aacaattaga agaagaattg gatagaacgg gagtaacttc
    34381 ccaaaattcc gagattgcta attgagacat tggcgcgatg aaacggtggg tctatatagc
    34441 gctattttta attatcagaa caagtgatgt ttgatcttca aaataacaaa taaaaaatta
    34501 aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc ttaaggaaag
    34561 ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa attggcgcag
    34621 ctggcgtaga taagttgtat taatggtgag actttcccat agatacaact ttttagagtg
    34681 catttctaaa gcttgtatct atgctagaca tgaacattga ctgccgcata gattagaatc
    34741 tatgcggttt tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt ttgtagactg
    34801 gatacttgcc tgattataat aaataaaaag attgactttg caggaggaaa aaaatgaaaa
    34861 acaaacgatt aataacagtt gtagtaatta ttttagttta tttagcaggt gcatttattt
    34921 atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa aaaaaaacag
    34981 ttgttggggt attgcaatac gtgagccatc cggcattaga tgagatttat cgaggaatca
    35041 aagatggtct gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa
    35101 atggacaagc cgatcagagc aagctagcca caatgagtca acagctagta caatctgatc
    35161 cagatgcctt gattggaatc gcaacacctg ccgctcaatc attagcaaac acgacttcat
    35221 cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg ggctggatta gtcgcttctt
    35281 tagagaaacc tggtggtaat gtaacaggtg tttctgatca gccgcctgtt acttcacaaa
    35341 tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt
    35401 cagaggataa ttcaaaatac caagtagcac aagcagaaga agctgcaaaa aaagaagggt
    35461 tagaatcagt aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg gttcaagtga
    35521 tgagccgaca agtcgatttt atctatgttc cattagataa taccattgcc aatgcgatgc
    35581 aaacagtggt aaaagaagca aacaaagcaa atatccccgt cattccatct gtggatacaa
    35641 tggtggaaca aggtggacta gcaacaatcg ggatcaatca atatcaatta gggcttcaaa
    35701 gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt
    35761 atacatttga tcaaggggat acagtgatca atcaatctca agctgatcgc ttaggaatca
    35821 ttgttcccca atcgatgaag gaaaaagcca aaatcattac agatgactcg cagcaagaaa
    35881 catctaagga ggatgacaaa tgatcgtatc agctatttca caaggcatgc tttgggcaat
    35941 attaggatta gggattttca tgacttatcg gatcttagac tttcctgata tgacgacaga
    36001 agggtccttc ccattaggtg ctgctgtctg tgtgacagct attactcatg gggtagcgcc
    36061 gattgtagct acattattag gcgtaggtgc tggcatgata gcaggactcg ttacggggtt
    36121 actttataca aaaggaaaga taccggttat tttagcaggg attttagtga tgtcaggatt
    36181 gaactcagtt attttgtttg tgatgcgaac accaaatctt tctttattga atcatccgat
    36241 tctacaagac agcttccatc gctttgggtt acctgattac tttgacacag tccttcttgg
    36301 cttcatctct ttgggcatct tgattgctgt actgctattc ttcttcaata cagatttggg
    36361 tcaaggctat attgcgacag gagacaacga aacgatggct cggtcgttag ggataaaaac
    36421 cgatcgtatg aaaattttag gtttaacttt gtcgaacggt gtcattgcat tatccggtgc
    36481 gctgattgcc cagaacgacg gatatgcaga tgtaaataaa gggattggcg taatcgtcat
    36541 cggtcttgct tccatcatca tcggtgaagt gatcttccaa gaattgacgc tagcagaaag
    36601 attgattgct attgttgtcg gcagtatcat ttatcagcta ttgattttag tagtaatcaa
    36661 actagggttt gacacgactt atctgaagtt gttctccgct atcatcttag ctatttgtct
    36721 gatgatcccg caattgaaga aagcactgaa cttaaaaacg ggttttgaga aggaggcata
    36781 gtaatgactg atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat
    36841 gaagagaaag tactattgga tgaaattaac cttacaatca gagaagggga cttcatcact
    36901 gtccttggcg ggaatggtgc tggaaaaagc acactgttca atacgatcgc tggtacgctt
    36961 caactcagtc aaggatcgat ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa
    37021 aaaagagcac gattcctttc tcgagttttc caagacccca agatgggaac ggctcctcgg
    37081 atgacagtag cagaaaattt gctacttgca caaaaaagag gtcaaaaacg gaaattccgt
    37141 ttacgtaact tgaaaaatca aaaagaagat tttttcagac tatgtcaaga agtcggtaat
    37201 ggcctagagc aacacttgga tactccagcg ggtgaattat ctggtggtca acgccaagca
    37261 ttaagcttgc tgatggcgac gatcacagct cctgagttac tattattaga tgaacatact
    37321 gcagctttag atcctaaaac agctaaagca ttaatgcgat taacaaatca gcggatcaat
    37381 gaacaaaaat tgacttgtct catgatcacg catcgaatgg aagacgcctt gaaatacgga
    37441 aatcgtctga tcgtactcca aaaagggaaa atcgttcaag atatttctga aaaagaaaag
    37501 caacatttga caatgggcga ccttttgcag ttctttgaag aagtagtgga ttgatttcga
    37561 cagaaaaatt cctttcagta gaaggacttg aaaactcatt gacatcttcc tttgattcat
    37621 cctatactta aaacaataaa caattaaaca tatttgggtg ttttgtagga tcacgtagtc
    37681 aatgatttta acaattaaat agaatattct ttgctctgac aagtaaacga tgagaaaaga
    37741 atttgctact ttgccaccta gacgtttcgt actttttcgt atcaaacgct aagtggcttt
    37801 ttttattgaa gtaagaacaa aggaggaata agatgatttc tggacaaacc aaattagctg
    37861 ggctctttgc tgatccggct tctcacagtt tatccccatt gatgcacaat actgcttttg
    37921 aagcaaacaa catcgatgcg gtgtatttag ctttccaagt aaatccaatg aatttgaagc
    37981 aagcagtcga aagtattcga acttttgata tgttaggtgt aaatctttcg atgccgaata
    38041 aaacagcggt tattccttat ctggatgagg taagccagga agcgcaattg atcggagcag
    38101 tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta taatactgat ggtgcgggct
    38161 tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg actctactgg
    38221 gagctggagg tgcagcaaag gcagtcactg ttcaagcagc gttagacggc gctgaggaaa
    38281 tcgtagttta taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac
    38341 tcagctatcg aacttcttgt aagataaaag ttcgtgactt tgccgatacg catcaattaa
    38401 gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac agatatcggg atgggaaaga
    38461 aaatcaatca gtcaccgctc ttagatataa gcatgatgca tgagaatcta gcggtttttg
    38521 atttgattta tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa
    38581 aaggatacaa tggacttggc atgttgatct accaaggagc aatcgcgttt gaattatgga
    38641 caaaggaaaa gatgccgatc catgtgattg aaaagctgtt tgattaaaaa aggataatag
    38701 ataaagatct tttattctcg cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa
    38761 gcatttattt cgttattttt tattaaatta tcaaaaaatg ataggctttt gtcgattggt
    38821 tatcgtatcc tttatttatc agtaagaaac gaggaggaac cacaatgagt cggatggatc
    38881 gctataagga catccataag aaggcgaaac ctctgaagcg ggatacgggg agtatttttg
    38941 ctagaagaga acgaaaacga acgcgtatgg aaaatgagga agatgctacc cgtatttatg
    39001 aaagagagca gcattcattt gaacatttga accaatacga gcaacagata tacagagatg
    39061 caccaagagc agaagaacaa caaaaaaata aaaaattttt caatcggcaa aagaagaaca
    39121 aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg attttgcttg
    39181 ttttgatcct ttattcaggt atctcatttt ttattggtaa atatgtagct gaacatgaca
    39241 caagcttaga ggcagcgact tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc
    39301 ataatatttt gatccttgga agtgatacac gcggagaaga tgccgggcga gccgatacga
    39361 tcatggtctt acagttggat ggtccttccc ataagccgaa actgatttct tttatgcgtg
    39421 acagcttcgt tacgatccca ggggtaggac aaaataagat caattcagct tatgcatacg
    39481 gaggagccga tctagtgcgt caaactttag tagaaaattt tggaatcgat tgtcaatact
    39541 atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc atgaacggag
    39601 tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt agatattaaa aaagggggcc
    39661 agaagatgga tggacatgtt cttttgcagt acgcacgttt ccgaatggat gaacaaggag
    39721 actttggccg tgtcagacgc caacagcaag taatgaacgc tattttcagc caattgaaga
    39781 atccattgaa tctgatccgt gcaccttatg cagctggtaa agctttagga tatacatcca
    39841 cagatgtcac tgcttggttc ttgacgaaaa acctcttttc gattgcacga ggagctggtg
    39901 gcattgatcg aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa agtgattatg
    39961 ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc tgctattaca gattttttga
    40021 ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc
    40081 aaacgattca ctcgtaaaaa acgctttctt atggtagatt taaccaagag aggagctgtt
    40141 tacattggat atctatctga acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga
    40201 aaaagttttt cagactgaag catcaggtat caccacatac ggtgaaatgc ctggaataga
    40261 acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc tgaacattta
    40321 tgatacatta gttatgtttg cagatacgcc agaaggaatt ggtcctgaat tagttgtggg
    40381 aaataatgtt tctttagtca tccagacaag aaatgaagaa gaaacgaagc gcctatttga
    40441 aagcttagct gagggtggta agattgagat gccactggat gctgttccgt gggctcagtt
    40501 gtatgggatg gtaactgatc gatttggcat caactggcag attaaccaag agtgacagct
    40561 aaagatgaat attttctgga aacatcaagc agcaaacgga tcatcgtttg tttgcttgat
    40621 gtttttgtgt ctatttcgac tatacggtat ctttttttta ttgccagatg cgaaaatatg
    40681 ttccataata gagaagtccg caaaaaatta agtaacggag gtcagaagat gaagacggtg
    40741 atactagctg aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca acagcaaaag
    40801 cggcatgaag ggtattatga aatcagcgat ccattgtttg ctggtgaagt aaccatcact
    40861 tatggttttg gacatttggt agatatggtt gctccaggag cttatgaaga gcgttgggca
    40921 aaatggtctt tagaaaattt gcctattttc ccagaaacct tccgttacga agttccaatg
    40981 gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc agtcagccga taccatcatt
    41041 attgcaacag atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca
    41101 aacgcatttt ctaaggaaaa gaactattta cgtttatgga tcaattctct tgaaaaagaa
    41161 gcaatctatg aaggttttaa gaatctccaa cctggaaaga actattttcc aaaatataaa
    41221 gaagcgcaag caaggcagaa cgccgattgg ctgattggaa tgaatggaag tccattatat
    41281 agcttgctac ttcaacgaaa agggattgat ggcagttttt ctcttggtcg tgtacaaaca
    41341 ccgacactct atatgattta tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg
    41401 tactttgaag gagaaggttt aattacgaca aacaaagggc agttttctgg aaaaatcgta
    41461 ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg aaatagaaaa acttggagcg
    41521 catttaggta agcaaaacgg acggattctt gaagttacga aaaaggaaaa gaggttgcat
    41581 agtccacgtt tattttcgct atctagtttg cagacaaaga tgaatcagct aatgaaagca
    41641 agtgccaaag ctacacttga tgctgctcaa ggattgtacg aaagcaagtt tctaagctat
    41701 ccaagaacgg attcctttta cattacagaa aatgagcatc agtatctggt taaacatctg
    41761 caaaattata aaaaattttt aggaatagaa gaagtacaga caactgagat caaagcaaag
    41821 aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg caattattcc gacaaagacg
    41881 attccgactc ctgcacgctt tgcgaagctt tccaaattac agcaagcaat ctatttgcaa
    41941 gttttgcgca ctactgtggc tatgtttgct gcagattata tctacgaaga gacgaccgta
    42001 atgacgggcg tacagcaatt aaagctaaaa agcatcggaa agatcccatt acagcaaggt
    42061 tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag ctcagactct tcctcctatc
    42121 gtagaaaatg aagaagcaga agtagaactg aaaagtatgg agaaagaaac acaacctcca
    42181 aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat
    42241 aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga agctacacga
    42301 gccaatatca ttgaaaatct taagcagaag cattacatcg aagtcaataa aaacgaaata
    42361 actgttacgc caaaaggaat cactctttgt aaggcagtag ccaatgaacc attactgacg
    42421 agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa acaagagggg
    42481 acaccatctg tttttttaga gaatatcaaa aaattcatcc tacatttatt ggcagaagtt
    42541 cctaatcaaa tcgacaaggt agacttttca gatgagatta gcgggacacg tcagataaaa
    42601 gcgatggaga agaaaaacga tcaattagga agatgtccaa aatgtaaaaa gggaatagtc
    42661 atgctttatc ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa attgtggcca
    42721 acagtagcta aaaagaagct gactaaaacg aatctcagag atttgctttc aaaaggaaag
    42781 acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc agttttagta
    42841 ttaaaagaag atatgacaat cggtttttct tttccatgga tcgaaaacag tggaacaaaa
    42901 gcagttgatg gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt agagtaaatg
    42961 aaaacaatga aatagcggaa tatttattcc gaaaagtgag aatataaata ccaataaaac
    43021 taaaaaatat cagtgaatca agcaaaaacg ttggggtagg ctgattttat atagatagca
    43081 aggagttttt ttatttgaaa aaagtgcatt catttttaaa ttatcttatg ggagcaatca
    43141 cgattgtggc tgtgtaccaa gcatttctaa agtatcctga ttacgggacg aagttgttgt
    43201 cattgacgca caatcaaaca gcatattcac caacgttgat tttatcgact ttgactgcag
    43261 gagctaaaag cgctacttat ttttttcata aaacgacaag catgctaaag cgaaagaaac
    43321 agcgaaaaca attgttggtg gtggtagcgg ctatgatcat tatttatttt ctaccagtta
    43381 ttacacaagt atttcatcaa tattttggtt aagaatacga gagtttgtga caaaagtcat
    43441 gtcacaaact tttttctttg tcattttttc gtaagaaaaa gcaaaataaa agtatataca
    43501 ttgattaaaa gtatatactt ggatataatg tacttactat agaatgagaa tggtatagag
    43561 ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc aatagcgatg
    43621 taacaactat cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca
    43681 gttatgtttt tcaatctgat ggttctgtac gaatgatcaa agcacaagaa gaacctgata
    43741 ttgattcatt agttgattcg atcatgaatc aatatgaaga cgcgttaaaa gatttggttg
    43801 atttatgagg tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg
    43861 tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat gcattagata tggccgtcaa
    43921 acagccttca caagcagtat tcaatcaaga actctatcct gaaatctatg aaaaagcggc
    43981 gattttagca attaatttag ctaaaaaaca tccttttcat aatggcaata aacgaactgc
    44041 tcttgttgcc atgttgttgt ttcttcaatt gaataattgt ccagttgctt tttctcgtca
    44101 agaagctgtt gattttatca taatgattac cactagttca ttggatttcg attcactaaa
    44161 gtccaagata accagttatt tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta
    44221 tttctcaatt agatcagcat gtcattaagg aatatgctga tttttttatg tttaatcata
    44281 caaaatagtt ttttcataaa aatatggaaa atatggatta attttgaaat aattgattta
    44341 aaaggaatac aaaagagtga atagtaggaa atagtggttt agaactagtg ggctgatttt
    44401 ttatatattt tagtttacat aatatatatt atacaaagta gatttaaaaa atgataaacc
    44461 tctctcgaac ggttttttat caaaaatcgt catttttcca tcaaaatctg cttatgcaaa
    44521 attataaaaa aattttgcag tttttcttac tttttttgtc gtttaattga atgataaaaa
    44581 ccagtttctt tattcagtta atcaaatatg aacgaattag atatgaatca ttgtatttgc
    44641 cgttgcggaa ggatttttta attaaccaca gataaacatt gataacgatt cgcttattgt
    44701 aatcaacaga ttaaatttca tatgttcata tgtacggata cattttcgga ccgattaatt
    44761 ttaaccattc tgtttatttg cttacctatt agatatcaat aaaaaactgc ttaacagcta
    44821 tttcagctgt taagcagttt taggattttt agttcatatt cattgtttct tataatacag
    44881 tgttcaatac ttctcttaaa gcacttgttg atttgctgaa cattgctttt tcttcttcgt
    44941 tgattgataa ttcaacaact tcacgaacac cattttcgtc aacgattgat ggcacacctg
    45001 taaagatatc tttttcgtcg tattcaccag ttaaataagc agatacaggc aatacagctt
    45061 gttcgttatt caagatagct tttacgatac gtgcagtact cataccaata ccatagtaag
    45121 ttgctttttt acgatcaatg atctcataag cagcattacg gactttatca gcaatcacat
    45181 ctaattcatc ttgggcaata cgatggtctt tttctacaat ttcaaacact ggtttaccac
    45241 cgacagttgt atgtgaccat gcagcaactt ctgaatcacc atgttcacct aagatatagc
    45301 cgtggacgct gcgtgggtca acttttaatt tcaatgcgat ttctttacgg aaacgagttg
    45361 tatccaatgt tgttccagta ccgataacac gtgatgttgg cagaccagat tcgttccaag
    45421 cgatatatgt caaaatatct actgggtttg aagcaacaac gataataccg tcgaatcctg
    45481 atcccatgat ttcttttacg atttggcgca tgattgacgc atttgtttta actaaatcta
    45541 aacgagattg accaggtttt tggttgatac cagctgtgat aacaacgata tcagcatctt
    45601 tacattcttc atagccacct gaccatacag ccacattttc atcgccccaa gccataccat
    45661 ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt cacatcgatc aatactaatt
    45721 cattagagat tccttggttg atcattgcat aagcgatact tgttccaaca aaacctgtac
    45781 cgacgattac tactttacga cttgtttttt tcatgaataa acagctcctt ttcataacta
    45841 tttgttactt tcatgaaaag tatagtcttg tttattcatt ttatcaacat gttaattata
    45901 atagttgtta ttataatttt tgatggaaac gaattcctga atacgcataa cagataaatg
    45961 acaaatgttt acacctatat cccctgataa tacaaccaaa agtttagaac tgaaaataaa
    46021 tccaaaaaaa ttcgctgcaa cgatataata agacagcaat cgcacctaaa acaattttcc
    46081 ttcatatgct ttagtgaaag attttcttct taatgtttat ttctccattc attgggagta
    46141 catgttgaat acttcttgaa taatttgaat aaagaagtcg tttgtttata tccgatacga
    46201 ttagcgattt cttccaacga aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat
    46261 tttttcttta gaaccagttc ttgaaaggaa gcatcaaaat acattttcaa tttattacta
    46321 agatagaccg gattgtagcc aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa
    46381 tgcttatcga tataatctaa tactctaagc atctcatttt ctgcgcatcc atctgctgat
    46441 gaaaagcttt gttgttctaa taaatgagtc aattcgatca gtaatagtga tgtatagaga
    46501 tttaacgaac ggatcttatg actgtatgta tcaaaataaa tcaacagcat attttcaatc
    46561 aaatgcgtca aacgacttgt ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct
    46621 ttttcttttg atgcatttat caaaaaagaa gaaagtagat tatttgtgct tgccaaatga
    46681 ttcaatatct ctgtagataa ggtttcttct tttaataaga tgttgattaa aatatcctct
    46741 tgccctgtgt attcgattct ttgagtagct tctttatcaa tgatcaccac gtcactttct
    46801 tccatctcta tcctttctcc attaataatt tgtaaacatg taccgctata catataattg
    46861 atttcaacaa aatcatgtgt atgctcggga acttccgcat atcgacggtg tttactgata
    46921 tgaatatttc cctgttgaaa tagatcccct cggtggattt ttggcggtgt ctgaagatgg
    46981 taagctaagt ttaaatcatt gatattcatt ccttgttcac gctgcagagt ttcgattgat
    47041 tcttcgttac gcaaaaaaaa tgtctaattc agctagttcc aattgactcc ctcctttttg
    47101 taagcggttt ctttcgctta ttagttcacg ctgtactgct attatatgca aagctatttt
    47161 tggtttcata aagactttct atggttggga catgaacttc ctgtgattcc gctattgaga
    47221 gaataaccac ttcatactat atttttgata agcgcttaca aaaatgaaag gaacatgctt
    47281 atgaataaaa agatgatgaa attttctctg ctgtcagtct ctcttatttt gacttctgct
    47341 ggtgcagtca cgtctgcaat tcccagtatg tcagctactt atgccacgcg ttctttatct
    47401 gctgttgaat tattaacgac tgctccctct tttatggtca ctatttttgt tttgattagt
    47461 agttttattg ctaggaaaat cggacaaaaa caaactgtga tcatcggatt agtgattgca
    47521 tcgatttgtg gtatcctccc cctcttctca caaagttaca gtattgtctt agcttcaaga
    47581 gctggactag gtattggctt tggattgatc aattctttgg cagtcagcat gattgctgcc
    47641 ttttatcacg gagatgaacg agcctctatg attggttttc aaagtgcatt tcaaggattt
    47701 ggtgcagctt taatgacatt tgttgcagga cagctagtac agttcggttg gcaatatacc
    47761 tttttagtct acgccatcag cttacctatt cttgtcttgt ttgttttgtt tgttcctaca
    47821 cctgaaacaa ctgaacttca atcgcatggc acagccccaa caagacaagt aatcaaaacg
    47881 aatcctaaaa tgattagtta tggtattttt ctcttgctgg tcatcgtggt atataatgct
    47941 gtatcgatta aattagccac agtcctgacg acttattctc taggaaacga aactaatgca
    48001 gcgaatatct taacaatcat gcaattggca agtatggctg ccggagtttg ttttggctgg
    48061 cttcagacaa aagcacatcg ctatatgctt acactttctt tactactgat gggaatcggc
    48121 tttgtattga ttgccactgt gataaacctg tatgtgattg ggtttggtgc gatcctaaca
    48181 ggaatttctt tttcgttatt cattccttac ttattcaacc aagtaagtat ccaagcgccc
    48241 aagtacgcgg agagtttcaa tacttcaatc ttattggttg gcgctaattt aggtactttc
    48301 attgcaccat atggtttgct ctttctctct tttgcttctc ttgggagtaa aacacttgct
    48361 gttttcgtca atggcagtat cttgctgttg attagcgcgc ttattagcct tctagtagta
    48421 cacaggacga atagaaagac agtgataaga gtagatgaag aaataaaaga acaattgaca
    48481 acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg
    48541 ctttggtatc ctggagactt tgagatccgc catggactgc tacaaaattt ccaacgggaa
    48601 gaacgaggtt ttgactggcc agcgtactgg tacatggatg attgccatcg gaacgtcaag
    48661 tttaagagat attactttct tgatcaacct tccatgttta aagtgacgat ccaaggagtt
    48721 gggtatgtag aaattaatgg acaaaaacat ccatgtggaa aatggctgac ttgtccagct
    48781 ggtaaagcaa aaattcgtat ctttgtagga catacatcag ggctaccagc gatgtttatc
    48841 gagggagacg aagtaaaatc agatattggc tggacagcaa gtaattttat tgaagaatat
    48901 ccagctggct ggagtccgct ttatgtcgat atcgcaaaag atccaaatca aatctattat
    48961 caaaaagaat gcgtccatcc agtcactgaa acggagattg gcggaggaat cttattcgat
    49021 tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc tggatgacca tatcgaatct
    49081 atcactctat gttatggaga gtccgatgta gaagctatgg atatcgatta ttgctactat
    49141 aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt
    49201 ataccagagg tttctactgg aatggttcaa cttcaagttt ttcatgaatt tttatctctt
    49261 tcgaaacaag ctgctttttc aagcgattct tctttactga atcagatctg gtcagtatca
    49321 gaagaagcct tcaagttatg tagtgatttg ttttttatcg acggtatcaa acgagatcgc
    49381 tggatttggt ctggagatgc gtatcaatca ttcatgatca atcaatatct tttttttgac
    49441 gaggaaatca ataaacgaac gatattagca ttaagaggac aaaatgaaat caagcagcat
    49501 atgaatacta tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa ccactatatg
    49561 atgacgaaag actatgaatt tttacgcatc gtctacccta agatgcaatc attaatgaga
    49621 tacttgatgg agcaaacgaa tgaattaggg tttatttatg ggagagcaaa agattggata
    49681 tttattgatt ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca agtcttatta
    49741 ctgaaagcct atcattccat gatcacttgc ggagatatct tagctgaaac atatggagag
    49801 aacgaaaatt atcatactca tctctatcaa gaaaaatatc aactattgaa acaaaaccta
    49861 tttgactact tttgggatga agaacttggc gcttttatcg attgctatga atctgggaga
    49921 agaaatgtga cgcgtcatgc gaatattttc gctgttttat tcgattttgc taacaaagac
    49981 caacagcaat cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca gattactact
    50041 ccatatttca aattctatga acaagatgct ttatgcaaac tcggacagac gaatatcgtc
    50101 taccaaacga ttcttgatta ttggggaggg atgttatctc atgatgcggt aactttctgg
    50161 gaagaatacg accctactca aacaggaaat gaaaagtaca gtatgtatgg agatccttat
    50221 ggaaaaagct tatgccatgc atggggagca agcccaatct atcttttagg acgctatttt
    50281 atcggtttgt atccaacaaa gccaggttat gaagaattta tgatacaacc taatctagat
    50341 tcatttgaaa cactgaattg tacgctacct atcaaagatg gaaagatttc gcttgaaaaa
    50401 acaaaagata agatcgtgat tacttctgat agaacaggcg gaacagtgaa aatgaatggt
    50461 aaaagttatc ctcttctagc aaaacagcca cttgtcttaa caagctattc aaaaataaat
    50521 gcataaaaca aaaaactgct tgcgacatat cagaagactt taaatcttct tctgatattg
    50581 tccaagcaat ttttattttg aagtttgctt aattctgttc tttagaaaca atgtttgttc
    50641 tttttccggc aaaaagaaag ctagataata cgacaagtaa aaagaaagcg atgattccaa
    50701 ctgtcaaggc tatttgaccg tattctccta gtttcccaag tacaagtcct ggtactagat
    50761 aatcagtatc ggcaaatgta gaagaagcga ttttaagatc tcctaatact ggaaggataa
    50821 ataatggcag ccaactaatc aataagctat tgaaagcaga acctaatacc gcccctcttc
    50881 taccgccagt cgagttgccg aagacaccag aagcagctcc gcagaagaaa tgtcctacta
    50941 ccccaggaat gataactgta gtttgcaagc ctatcatagc aaacatggat agtgttccta
    51001 caaagaagct aacaaaaaag ccaattaata cagcattagg tgcatatgga aaaacgatag
    51061 gaacatctaa tgctggttta gaatcgggta cgagtttttt agcaatccct tggaatgctg
    51121 gaacgatttc tgccaacacc attcgaaccc cttgaaggac gataacaaac ccagctgtga
    51181 atgttccagc ttggatcagt gcaaagatta cagcgttgtc tccattgctt agttctcttt
    51241 caacaaatcc aggaccagct aacaaagcaa gtgcgacata tacgatcgac atcaagagca
    51301 tgatcgatac agtagaatct cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg
    51361 tagaacggtc tttgtttcca aaaaactttc cgatccatcc actagaagca taaccgatat
    51421 ttccggtatg ccctaacgca acttgattgt ttccagtgat gttcttcata aacggttgac
    51481 aaagagcagg cgtaatagtt aatagtgttc cttcgaagac cccgcctaat aagatcatcc
    51541 acaaatttgc agtaaacccc gcgcttacta aaatgactgc tgttagacaa gaaacataaa
    51601 gcattgcttg acctgtaaga aaaatgaatt tatatcttgt gaatctagca agtaaaatat
    51661 tcacgatcat ccctactagc atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa
    51721 tcgccactac tgcttcattg ctaggaacta ctccttgagt atgcagagct gtttggaaca
    51781 tcgaagcaaa aggggttagt gaattactga ttaaaccagc accgccgatc agtactagga
    51841 atccgacaaa tgttttgata gttccttgga tcacttctga aaatttcttc ttctgaagaa
    51901 cgagaccaag cagtgcaatc aaagaaacga ggatggaggg ctgactgacg attgacacaa
    51961 acaaattcaa caatttatcc attgatagtt cctcccttga atcattttta ttcttttgac
    52021 aagatcttcg ttactttttc ttttacttct tcgctgtcaa tgatcgattg caaaggtacg
    52081 agcttttcat caggtataga agttagcgta tctttcaaag aatgctcaat gaagaaataa
    52141 tcagccatat cgctagatac acttccaaca tccgtatgac ttacttcaaa ctcgtccaca
    52201 tctgctccaa gatctgcaag tgcttgtttg atgttcatct ctaccataaa acttgttcct
    52261 aatccacttt gacatactgc aaaaattttc atatcattcg tctccttttc ttatgatcgc
    52321 taatatttct tcttttgttg atgcgtgtaa taaatcttga agattttgtt tattagaaag
    52381 tagctttgtt aaatttgcca aagcccgaag atgtgcctca ttatcgatag cagctaaaca
    52441 gatgaacaaa ctaatgggat gggctggatt atcaagcaga tccacaggtt gttgaagttt
    52501 cagcaaagac atccctactt cgtttacgcc atcttctgga cgagcatgag gtaatgcgat
    52561 gtgatcgcca atatggataa acgcaccata ctgctccacg cgctcgatca tagctttgat
    52621 ataactttct tctattttat tttctgataa tagcggctgt gcggcaaatg tgattgcttc
    52681 ttgccagttt tttggttgat ccgataattg gatcatttct tctgttaata attcagatag
    52741 cataggacgt tgatcctcct tcttttctaa aagtttgttc attttacgat ttaatacttg
    52801 atagagcttt tcttcgtcta cgccttcttt taattcgata taaggcagta gcgccgacaa
    52861 caattcttgg gcagaaggca ttgaaaatcc tggtatcagc caatctcttt gtacttgatt
    52921 gatcagctga tttttttcca agtcagtcat gattggatgc agaacataga gagacttttt
    52981 cttatcagta agtgtgagag gtacagtaga aaaaataata tcgtaagaag tttcgtcaac
    53041 ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag atcatcggga ataattgctg
    53101 aagttcactt tttaagatca atgaagagct aatgccactt ggacagacga tcacggctct
    53161 tatttttcta tttctttctt ctgcgtccgc ttttctgatc tctccgccaa atagtatagt
    53221 gaaaaaacca atctcttctt cagggatgct cttccctatt cgtctttcta acggaagtaa
    53281 tgcttttctt gtcatttcgt agatggaagc atattgatgc ttaatttgat cgatcatcac
    53341 attaggtaaa taaaaagaat aggaaatacg aaaataggca gggaccaagt gataaaagac
    53401 attcatcaat aattcacgaa acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg
    53461 gatgacttct gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga catctccttc
    53521 aacgatcgat gctaagcaaa gagagaaaaa tataatctcc gaattttgat gttcggaata
    53581 gccaaggcca gtcaatactt gtcgactaat gtcagtgact gcaagagaag ccaacagttc
    53641 agcatctttt accagttcca tggatgtttt tccaattcga atggttgtca aactcaaata
    53701 ataagctaat tcatccattc gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt
    53761 atctagcaaa tctctaactt tggcgtaata atcaatggaa agggttgcgg ttaaatacgt
    53821 gagcccaaat cttccagcaa gtgactgcat caaacgagca gtgcgatttt ttgcttctct
    53881 tcttaatatt gcttctgacc ctagcagcct aaaaccagtt ttcctagtat acgacagctg
    53941 acaatcttcc acttctaatt catttcttat ctttttgata tctgacagga ctgtcccttt
    54001 agaaacctga agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac
    54061 catcaaataa agaagatctc ttcgctcttg ttcggtatac acagcttcaa atcgagtaat
    54121 atccaaaaat ttttctgccc agtcgattgc tcggaatttc tttatacgga tacgggtatc
    54181 atccacatat tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat ttacttcgat
    54241 gatctctgct gctttttcta gactgatttc tcttggtgtt ttcaggagat aactaatttt
    54301 gtagttcaca gcgctgttca cctcctactt atattctagt atggtagcgc ttttaaatcg
    54361 aggggtgttt ggtacaactt acagttggat tttttttacg actgtaccga acgatttcta
    54421 tcgaatggat acagaaaaaa agcaggtgac caaatgtctc ctgcttttat gaattgattt
    54481 cgtccgatca atgatgatca tgtttatttt ttgaggtcag ttcttcaaat ttttctttga
    54541 catcttcaac cgctttttcg atatcttttt caaagtgatt gggctcatta ggctttgttt
    54601 cctcttctac agaatttgaa ccagataaag ctgtttcttt attttctgtt tcaccagagt
    54661 ttgtgccagc taatgattct tctttgtctt ctgtttcacc agaattagtt cctgctaaag
    54721 ccacttcttc ttctgtttta ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt
    54781 cataatttgt catgttggtt cctcctagta gttctatact ttttcatcgt acaataagac
    54841 agtttatgcg tcaaaaaaca aacccgattc ttacaaatat gtcatatttt tccttctgat
    54901 tcttgaatgc ttttgattcc ttcttccaaa attttcattt gtaagattgt ttcttcatct
    54961 ttgatgactt tttctgcgcc attaactaat gtctcgtaaa tcccttcgta gacacggctg
    55021 taatcaccaa cctcggaaat tactttttct tcatgataac tcccttgttc atctatatag
    55081 gtcaatacac cataatgttc gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa
    55141 aacattttca gatgttcttc ttgtcgatct tttgtttgtt tcacgaacat cccttttttt
    55201 ccgtaaacga cgaaacttgg acgttctttg atgcggaaat aactggattt tatcgaaatt
    55261 ttcatatttc catagaaaaa atccagatcg aagtaatcat tcattcgtcc tttccctaaa
    55321 agttgacgta catcatagct gatatcatct ggttcgccga aataagaaag aacttgatct
    55381 actgtatgac aagcatgtcc atataaatag ctgttcgcag tagaaaattc atgcgtattt
    55441 tctggtactt ccggtctgaa ataatcataa tgcatttcta cttctaaaat atcccctaat
    55501 tttccgcttt cgattacttt ttggactgtc aagaagtcag aatcgaaccg tctgttttgg
    55561 taacattgga taaatagacc tttttctttt gctaatgcaa ataactcttc tgcttccttt
    55621 gacgtttctg caaaaggttt ctctactagt acattcttac catgttcgag tgccattttt
    55681 ccatattcat aatgaaattg actaggtgta gtgatgacaa ctaattggat ctcaggatca
    55741 tcccaaagat cagacagctg atcagtatat tcgatgtctt cgaatgtttt ccacactggt
    55801 tcagatgggg aaaaaatttt tttgatattg attttgttcg taagttttct tgaaaaaggc
    55861 aaatgatagc gatttgtact ctttccattg ccaagataag cgattgtaag catgagtggt
    55921 gcctcctttt attgatgttt tcagtatatc ctagaaaagc gcagaaagta tttataatag
    55981 ctattctggt aaaaaagttc agaaaaacac ttgttatcat tcggtttggt gaaattaatg
    56041 ttcaaagtaa ggataaaaat caaaaagata gtagcggaac caagtgttta tcagtaagat
    56101 agaatttgga ggtcagatca tgcttattga agaaaaacta acaaaacaag agttgtttac
    56161 cacgacagag aaacggattg tcgattacat ccgtaggaat atcgaagctg cagtctacat
    56221 gacgattgaa gaattagcaa aagctacgta tacttcccat tcagctatca ttcgtttatg
    56281 caaaaaaatg ggattcagcg gatttaaaga attccgtttc gagctggggc gggaagttca
    56341 tcaattgatt gctcagttca atcaaacgat tgatcctaat tttccttttt caagtgaaga
    56401 tagcactcag caaattgcaa agaaaatggc ggatttatcg atccagtcaa taaaaaaagc
    56461 acaattgcag attgaacatg aagatttaga tcaaatcggt aaagtattga acaaggcaca
    56521 gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg gcaaggaaat tccagaataa
    56581 aatggtcaag ttgaataagt ttttgatcat ggctgaagaa tacagtgatt cttcttggaa
    56641 tgcagccaat ttgactagcg aggattgcgc catttttatt agctacagcg gtcgcatcca
    56701 tcattatgaa cgaataatga cctatttgaa gcatgtagag gcccctacct tgctgattac
    56761 cggaaatcag cattcagaaa tggccaaaca ggctgatatg tgtcttgtta tatcgcagga
    56821 ggaatatgat tttgctaaag tagcgacttt ctcctctcaa attgccttcg attacgtatt
    56881 aaatacgtta ttctcagtga tatattctca aaattttgaa gagaatatcc gcaacttgaa
    56941 aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa
    57001 gatcctcatt tagtaataag aaagatagtg tttgataggc tctctttgat atcatcataa
    57061 aaacaggctg aagttttctt tttcaaacct cagcctgttt gtttacggaa cgattcgaat
    57121 agatatatta tagaaatgag gaatagctaa cttctgtttc ttattttgaa cgattgacga
    57181 aaaagatata taagaaggag gaaaatcagt atacatgcag ctaacaatcc atacaggaaa
    57241 aatcgtttgt tctcggatac tttctgtact tctttttgaa cttcttttgc atgataagga
    57301 acccggtgcc ctctgacaag taagcgatga ctgttgacca tataaggtgt acaagttagg
    57361 agtgtcacga aatcctgccc tttttcgatt ctcagatctt ttgtatcagt cggttctacc
    57421 gtcttgatct gatcgacttg ataagcgagt gtccgtccat tgatttcgat atagaaagaa
    57481 tcacctaaag tcagtttcgg taagtctgtg aacaactttg cttgaggcag tcctcgatgg
    57541 ctggaaataa cggcatgtgt attttctccc cccgtaggat aagacgttcc ttccaataat
    57601 gaggcacctt tttctagcag cacagtattc gtttcatcaa aaattggcaa atggacattg
    57661 atctcaggga tcgtcaaaat accgatcgta tgtttctcaa aataggttct atcctttttc
    57721 gcaggttctt ttttcttagt aaagggatct gcacctggat ttccaccttc ttttgctaat
    57781 tgctggtttt tctttgtcat gttttcttgg attttagcca tgacttcttc attttctttg
    57841 accgcttgtt gctgataatg agagatgatc tgctgatcca gataattatt cagcgcgtcg
    57901 ctaacaaatg gataagctag tatccctatt cctgagagaa gcaagaaaag catcagcaga
    57961 tctagaagtt tccttttctt tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa
    58021 taaaagaaaa aggagcattt gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga
    58081 ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca gcaatcagta gaaggactac
    58141 tcctgcaccg atatacacat agattccctt accgcctgtt gaaggaagtg tacctttgtg
    58201 tttatttggt attttttcag gagaaatcaa ctgtcctgct gttacataag attgttcatc
    58261 gatagtaaat gctacacggt ttgttaatgg aacatatttt tctggcgctt tcgtttcttc
    58321 cagatagtac gtgccatatt ttagacctgt cacatcgatt aaaccgttac ttgtggttgt
    58381 aaaaaccgtt gctgattctt tcgccgatac ccagctgacg gcttttgtgg atgggtcgat
    58441 cgataaatat ttcgctgtgt cactatcttg atcacgaacg acgaattctg ctccagcaag
    58501 tgtttggtct gatgtaacgt caccatctac tttaacaaat cgtttgcccc cagtaacgac
    58561 atcgactgac ggtggtgttt gatcatttgt atgcccgtta tcgacatttg cttggttaga
    58621 aaatcctttg gttggatctg ctttttcgtt caaatgcata tagtaaacga atttcaatgt
    58681 accgccagga gttaatgaag gaatataatt cggatcaact gaaaccgtga atccgtctgt
    58741 ttgctcagtg acagaataat ttactgggtc gatttcttta tttccatcat ataaggcata
    58801 agcatacgtt cctgaagaat cattatcaaa tgttaaagca gcgtcatgag tatcgataag
    58861 tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca gcgataccta atgggatatt
    58921 gacagaaatc tcatattgaa ttttttcacc gattgcgaca tcttttccat tcaattgagg
    58981 tgttgtttta tcaactttag acgtatcatt tttgatggtc ttttctactg gtgtttggct
    59041 atttgccagg atctcaaaag gcgtgattgt ttgcttttca atcatttccg cattatcagg
    59101 agcttttact tcttctaaat tatattttcc aacttctaaa tgattaacga tcaactggcc
    59161 tttttcaata gatgcctcgg caaagtcatt gttgccgatg tcataagaat gaccagtaat
    59221 gaaatgtttg gctttggttt gatcagttgt ccaagtgtac aatccatctg tgacactttg
    59281 gatgtatttt ttgacgcttg gtgttccttc ttctttagaa ataataaatt ctgctccatt
    59341 tagtgcttcg ttttcggcag taccgatttt tgtaactttc aacgttccat cattaccgac
    59401 tgtattttta gggtagagat ggatagtatc tagttcttct gtcccgtatt tataagagcc
    59461 atctgcttgt ttgatcatct catatacagg gaaagctaaa accatgtttg cggcagctga
    59521 cactccgtct tttggttctt ctttgatcgt gtagactgca tctttcccat tttgtttttt
    59581 aggtaaagat aaagtgacat ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc
    59641 aggagtcaaa gactggactg cttgttttgc tgcatccacg gacgctcctt tatctcgttg
    59701 cgcataaaat tcttgagtga cgttataaac tgaaaatgaa atatcggcta atccttggta
    59761 ttgatcgaat tcgctcattt ctttcccgct gttttggatt aaaggatcgg gtaaatcagt
    59821 cattttcttt ttatgaagga tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc
    59881 gccgaagctg tttgtgagta atggtaaaat aaggaaaagg actcctaaca taacgtttat
    59941 ttttttatga tttttcattt tttcactcct gttctttttt tgataaacaa ccagattgca
    60001 aagcttgcga ataccatacc aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt
    60061 ggaagaaatg gacggctatt ttctttttta ttgactactt tcaatatact gtccgcctct
    60121 ttatcctcag aaagaaaagt tgaaatggta aacggaattt ctgtttcact taaaatataa
    60181 ccagaaggtg cttcgatctc ttttaatcga taacttcctg ttttcaaccc ggaaattgaa
    60241 aaagcgccat ttttatcgga aatcaggtga agcgcatctg attcgttctt tgtccacttg
    60301 tatccgttgg ctgtttgctg gaggtaattt cctgctttgt ttttaataag gaatactgcg
    60361 ccagctaatc gtttatctgg tttatttgtc tcgattttga caaattgctt actccctgta
    60421 tatacagaga ctttctttgt actgacacca aaaccaaaat ccagtgttcc gttgttgttg
    60481 atttccttgt tcgcctgtgc tgtactgctt agctgcatct gataagaaat ggtcagtttt
    60541 tttccagcat atttttccaa cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc
    60601 ttgtgtatga caaagccttc tgtcagcttt tctccattcg atgaaatcgt taaactatta
    60661 ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt cgaacttttg atagtcaagg
    60721 atatttacag gaattgtcgt atgcaaggaa taagtgattt tttcaccaat cgtaaaatca
    60781 tttctaggct cttctaatac cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt
    60841 ttaggataca aatggatagt tgaaagtttt tgattgtttt gtccatgtac aggaagaaca
    60901 actaccatat tctctgcctt ttctttgacg acttctggta ctttggattc aatgaaaaga
    60961 tacgctttat ctcttttttc ttgatcttta gcggccagag aaaaagaagc gataccatct
    61021 tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca taccggaaga cgcaccgttt
    61081 tttgcgatct ctgcttgtgc ttcttctacc gttttgccct tttcccgtag atggtaaaaa
    61141 gaatctgtga catcataaac ttggaatgtg acaccattta atcctcgata cgtttgtaat
    61201 aaagcttttt cttgtccgtc atttggatga tttttcggca gttgcccgtt tgggaaaagc
    61261 aatttgtgta aagtaatctg aaccatttct gtttctgttt ctgttgctac ctgagtaaaa
    61321 gctggtttaa atagtaacaa gaagagacac atactaagcc aaccaagttt tttcatattt
    61381 agctgccccc tctttctttt ttcaaaaaaa gataccaaat agaaaacgca caaccaatca
    61441 tccctactag atagattcct aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg
    61501 cctgattcgt gatgtctaaa gaaatctggt tgtttttggc tcctggtgac agaacagatt
    61561 catgatcttg tccatccact tgaattgacc catcttcgtg tataactata gtaactggct
    61621 cttttagacc ttggtatcct tctggtgtaa aagtttcggt cagcgtataa gttccaggtt
    61681 ttaaattttc gaatagaaag gtatctgttt cttgtccatc ttttggcgat tctaagtcca
    61741 tttctggtcc ctgcagtctg aattttgctc cttttaatgt ctgtccgttg tcttcttttt
    61801 tgtgtaccgt tagatcaaac gcttttagtc gattttgatg agtgagcgtc catacattat
    61861 ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc atatttatcg atttggaatt
    61921 cataatttcc cggcgaagta tcatatccgg caggaccagc tgtttcaacg actcgatatt
    61981 taccaggtgt gagtgaatca aaactggcaa gacctttttc attcgttgtt tggctgtcaa
    62041 ttggctgata agtaccattt gcttcttttt tctgtaggga aaatgctgct cctttaagat
    62101 ttatctcttt gtccgtccct tgcatcgcat acttacgtac tgctacagga acttcaacaa
    62161 aaggattttc aatagtcaac tggatcgtat catcggaagt agtgactgtt tttccatcaa
    62221 tggttaccgt accatcagaa gcgatcttga tttcccaagt agtctttttg cttaatccat
    62281 gcccttctgg agcttttgtt tctgtcagcg tataggtcat ttctttttgc aatttgacat
    62341 tttctggaag agaataggtt ccgtcgccat gatctgttag taaagtatca atagaatctc
    62401 ctgttaattt gaaaacagcg ccaataagat ccttttcacc tgtagaggaa tttttcgtta
    62461 ttttcaagtt taacggtgtg aattgtttag tatttttcca tgtcattgca tctatttgtt
    62521 gagaatcgta ttctggtaca ggtaattctt tgattgtttg gtaactgaat gcttgacctt
    62581 gattattgta ttgaggtagt gataatatct cttgataact ttctccgcta tttgcagata
    62641 atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc tttagctggt ttaatgattc
    62701 gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag
    62761 taacttgatc tggacgatca gctagattgt tgtcaaattc ttcccataac tttttgatgt
    62821 gaagactgac tccgggagct ttagcagatg gtataccgaa ttcagctaat tcattggtat
    62881 caatgtttgg ctggaaagtt gtcctgccgt tcatttgata ccagaaattt ggatggaagt
    62941 cctcattttc tgtttggatt ctcacttggt aatggatttg gatttcttgg tcttttccta
    63001 aatagatctg attgctcttg atggtatttc cttctatgga aatagatgga gtgaccgttg
    63061 taggacttgt ccccacactc ttgactgaaa gtgtaccagg ctgataaatg aatggttctg
    63121 cgattggatc atttatttgt ccattgctta cagttgctga gatctgtaca gcttttttgg
    63181 ctaggtattc agagatatct gttgcatgat cagctgattc atagtaaaga tcgccttttt
    63241 catctgatga aaccatttgt cgcatacgag actctacttc tgcttttgag agaccagctg
    63301 ccggatcgct ttgaagttgg atgccaagac catgtatttc gattcctcgt tctttgagtg
    63361 ccatcgcttc tccgatggtt gcgataaacg tactgtcgat tcttctggat agattgtttt
    63421 ggtcaggtgc atcatagatt cttgagatta gagaagtatt tcccggccga tcttgcgtat
    63481 tagaaaactg agttccgtaa taattgctgc ttgattgtgc gtgtacccgc tgtactttat
    63541 aggaaaatgt tggtacacca tccgtcagca aaacgatcac ttttttatgt ccatttggaa
    63601 cggatagcat gcttcctgca tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc
    63661 gtgaaggtgt aatggatttt acttgatttt tcactgaatc aaatgaaccc atctgtactg
    63721 caccgttact gtagctatat ccttcgcttg agtagccgac atatcccata ttgattttgt
    63781 ctgtgatacc gctatctgct aaagtatcga caaaacgatc gacaccaatc tttacttcac
    63841 cgatccgatt attgtcgttc atacttcctg accagtctac gaccaatacg agatcaagag
    63901 gcgtgatatc tttttgtaca tttccacgag cattcaaata aacatcaaac aatccgggtg
    63961 tacttgtttc ctttgcatac tttcgtatcg catagtctgc ttgattaccg gttcctccat
    64021 attcgatata cgaatgggtc cgatcattag gatctccatt ccaagaattt ataccatccc
    64081 attgatttgt tccgttttta ttgccttgat gatcaagaac atttgtattt cctgtaggtt
    64141 gccaactggc tttaggataa gtgccctgat catctgttgt atattctgta ggaatcgttt
    64201 gattagatgg cacactcaca gctggcaagg ataatgtagg caatgtaaat gggctgacag
    64261 aattttcgtt ttcttttgat gattcttctt ctatttcgtc tggctttggt actgacacat
    64321 ctgtatcttt cttattttct gtctcatcaa taatgtcata ttctttatta tcgacgatca
    64381 gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg aaaactcaga tcgatagttt
    64441 gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct atttacctga taagcatagc
    64501 catctccttc ttttctttcc acatcactta agtaattttc gactagacca atttcttttt
    64561 ctatcttttc agcactggtg ttattcgcat ttagcttgat gttcagtgct ttttttccag
    64621 actgatccga aatgacttca gctgaaattg tttctaaaac tgttggatca tctgtagtgt
    64681 ctgctattgc agataatggc acaaagtttg ataaactcaa tattatcagc atgaaaataa
    64741 ctttcagttt cttccctgtg gtcgtcattt ttcccctcct ttttcttttt ttctgttttg
    64801 acgtgaatag tgaaaattat taaaataaac aggcgttaca taattttatt tattttaata
    64861 tgataattat tatacgaagg gcgctaaagg gaatcacacc aattttcata ccaattggtt
    64921 tcatttatga agttgattcc ttcgattaat gagcgtttca ttgaaagaaa agggttgagg
    64981 gagaaaaaca tatgatcagg ttgttggaac gaaactatag aaagatggaa aaaatcattc
    65041 gaattctgtc catttctgac aagtggatga ctaaaaaaga acttgccaaa cgaattggta
    65101 gttcagaatc tacatttgta aggtatatcg aagagatcaa aatccgctgg ggaacggatg
    65161 taacgattca atcttcacaa aaattggggt atcgtcttga acatttgaat gtaccagctt
    65221 atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga
    65281 ttattgtcaa tccgggtaaa acgattgagt attattgtga aaaaatttcc atcagccggt
    65341 cttcttttac acgtaagttg aaacaatgca atcaaatttt agaaagctat ttattaaaag
    65401 tgatcgttga ccaaggtttt cagttaacta gccttaaaag tgagctccag ttgcgtatct
    65461 ttgttacctt tttcttttta agttactacg gaagacgcac gttgccattt ccaatcgaaa
    65521 aaaagaagat caaagaaatt atggtcaaga ataattgtca gatgaatttg tttcaggaag
    65581 aaagcccata tgagcaatct ttctttatta tgtattttgt tgtgcatctc atgagagaat
    65641 cccaaggaac tccatttgaa tggaaagctg gagactctta tgtacagttg ataaatatcg
    65701 ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa attgaagtac agcagctata
    65761 taaaagtagt gaactatttt gtcaatacga ttttttcaga attttcggag aaagaaaaaa
    65821 atgaaatcag tgagcagata cgaaaaaata tgtcctactg ttcgttttca tccgctgatt
    65881 gcaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat tttttctccc
    65941 gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt gcacagcaga
    66001 tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa ttttattcca
    66061 ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg atgcttatcg
    66121 gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc agcgacttaa
    66181 gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct gtgcataaaa
    66241 ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat cttctcgagt
    66301 atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat atattgattg
    66361 atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag ttatgaataa
    66421 tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat acacatagct
    66481 gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa gtcttttaac
    66541 aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat tacgagggtc
    66601 atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa atgattgatt
    66661 cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc tctacgtttg
    66721 gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata gatactttcg
    66781 tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg
    66841 ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct tttgatcgta
    66901 tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg cgacatcccc
    66961 atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca aaatatctcc
    67021 agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa agtttctttt
    67081 ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat tgtcatgaag
    67141 gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc gataaacgcg
    67201 atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt tttgtttctc
    67261 atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat ccaaactagc
    67321 tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag caattgccac
    67381 tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg actggatatc
    67441 taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag taacttggtc
    67501 aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc tttggagaat
    67561 aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct cagttagtga
    67621 gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg aaatggtaag
    67681 gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc cttcctttac
    67741 ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa atgttttggt
    67801 aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc tgtaattggg
    67861 tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac aatgatcaaa
    67921 atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa
    67981 atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac cccaacaata
    68041 aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg tgctttcatt
    68101 actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca
    68161 gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg ggcagaatag
    68221 ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt cgatgctttt
    68281 tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg ttcttcttca
    68341 gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga cgcatagatg
    68401 acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa aggatcgtcg
    68461 taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag attttgagag
    68521 atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg taataaaaat
    68581 gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt gattgctccg
    68641 ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc tcctcttgtt
    68701 aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc
    68761 caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc tgacaacatg
    68821 aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg taatttagaa
    68881 tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata gattgatagt
    68941 gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca cttgtgttgt
    69001 ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac atgagtcata
    69061 aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac caaatcaatg
    69121 aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat ccaataatat
    69181 aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta gtgaaggact
    69241 gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga tgccggaaat
    69301 gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc cagtattgat
    69361 tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag gtgcagatga
    69421 ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag cattattgaa
    69481 acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca gttgtgattt
    69541 aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa aagagtttga
    69601 attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc agatatttga
    69661 ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt atatgagcaa
    69721 cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct tacgtaatgt
    69781 cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga aagcaacgta
    69841 gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt ttgggattca
    69901 ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca ttagagaaaa
    69961 taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa ggcgatccct
    70021 ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca ttaatggaac
    70081 catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt gagatattga
    70141 ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa
    70201 atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct gccctctctt
    70261 tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac atccaaattt
    70321 tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt atgattttgt
    70381 gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct agtctaagca
    70441 tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat gcttctcatg
    70501 aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt accaagccta
    70561 atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag acgaggcgcc
    70621 tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat cagcagctgc
    70681 tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa ccatttcaag
    70741 agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca ttagaagtac
    70801 caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat gcattaaaat
    70861 atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa gaatggctga
    70921 ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg atttttgatc
    70981 gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg cttggcttgg
    71041 caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc aattatcttc
    71101 cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa tgcttatccc
    71161 taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa taaacggtgt
    71221 tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca cttctgtcac
    71281 aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta catcaccgct
    71341 tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa catcttcatg
    71401 ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta catctacttg
    71461 ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg ttccctgtga
    71521 agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga ccggtacttc
    71581 taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag ttgtcacatc
    71641 atgtgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat ctagatttgg
    71701 tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc caggtacgtc
    71761 catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa agcctgtcgt
    71821 gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc ccattgtgct
    71881 tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg gctgacgata
    71941 gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt gatataaagg
    72001 atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag tatcttttat
    72061 tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg tttcaacatc
    72121 tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag tataaataac
    72181 gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata atgcttcttc
    72241 cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa ttcctcctag
    72301 caaatagatt tatgattggt atattgttac ttataaattt attctatagg agacgattgt
    72361 tttctacaaa tatttagatt ggaatttgat taaaacaatt taattgaaat ttgaaaacat
    72421 ttatgttaat gaaagtcata ttctaataaa atgtcatttg tttctatatg aatttttatg
    72481 aaaaaattca tataatttcc actcgttgtg atggtttaat ttatccaatc catcaataag
    72541 caaacaactt a
//
LOCUS       DAFBYI010000002        69787 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFBYI010000002 DAFBYI010000000
VERSION     DAFBYI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.002"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..338
                     /locus_tag="LLD04_000066"
     CDS             <1..338
                     /locus_tag="LLD04_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5647033.1"
                     /translation="WVSNHGGRQLDGGPAAFDSLQYVAEAVDKRVPIVFDSGVRRGQH
                     VFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVFDFFKTELEMVMQLAGTQTVEDIKK
                     IKLRENRFI"
     gene            622..1302
                     /locus_tag="LLD04_000067"
     CDS             622..1302
                     /locus_tag="LLD04_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5647034.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1392..2426
                     /locus_tag="LLD04_000068"
     CDS             1392..2426
                     /locus_tag="LLD04_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK5647035.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2440..2775
                     /locus_tag="LLD04_000069"
     CDS             2440..2775
                     /locus_tag="LLD04_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647036.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2759..5239
                     /locus_tag="LLD04_000070"
     CDS             2759..5239
                     /locus_tag="LLD04_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647037.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5295..6854
                     /locus_tag="LLD04_000071"
     CDS             5295..6854
                     /locus_tag="LLD04_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647038.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6851..8215
                     /locus_tag="LLD04_000072"
     CDS             6851..8215
                     /locus_tag="LLD04_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5647039.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8386..9456
                     /locus_tag="LLD04_000073"
     CDS             8386..9456
                     /locus_tag="LLD04_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647040.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9471..10139
                     /locus_tag="LLD04_000074"
     CDS             9471..10139
                     /locus_tag="LLD04_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647041.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10159..10737
                     /locus_tag="LLD04_000075"
     CDS             10159..10737
                     /locus_tag="LLD04_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5647042.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11140..12051
                     /locus_tag="LLD04_000076"
     CDS             11140..12051
                     /locus_tag="LLD04_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5647043.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12307..13224)
                     /locus_tag="LLD04_000077"
     CDS             complement(12307..13224)
                     /locus_tag="LLD04_000077"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK5647044.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13426..13863
                     /gene="zapA"
                     /locus_tag="LLD04_000078"
     CDS             13426..13863
                     /gene="zapA"
                     /locus_tag="LLD04_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK5647045.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13921..14469
                     /locus_tag="LLD04_000079"
     CDS             13921..14469
                     /locus_tag="LLD04_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK5647046.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14584..16944
                     /locus_tag="LLD04_000080"
     CDS             14584..16944
                     /locus_tag="LLD04_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK5647047.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17031..17345
                     /gene="trxA"
                     /locus_tag="LLD04_000081"
     CDS             17031..17345
                     /gene="trxA"
                     /locus_tag="LLD04_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK5647048.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17527..19305
                     /gene="uvrC"
                     /locus_tag="LLD04_000082"
     CDS             17527..19305
                     /gene="uvrC"
                     /locus_tag="LLD04_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK5647049.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19450..20349
                     /locus_tag="LLD04_000083"
     CDS             19450..20349
                     /locus_tag="LLD04_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647050.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20342..21604
                     /locus_tag="LLD04_000084"
     CDS             20342..21604
                     /locus_tag="LLD04_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647051.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21767..23665
                     /locus_tag="LLD04_000085"
     CDS             21767..23665
                     /locus_tag="LLD04_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK5647052.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23964..24683)
                     /locus_tag="LLD04_000086"
     CDS             complement(23964..24683)
                     /locus_tag="LLD04_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK5647053.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            25020..25352
                     /locus_tag="LLD04_000087"
     CDS             25020..25352
                     /locus_tag="LLD04_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647054.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25339..27342
                     /locus_tag="LLD04_000088"
     CDS             25339..27342
                     /locus_tag="LLD04_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK5647055.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27361..27831
                     /locus_tag="LLD04_000089"
     CDS             27361..27831
                     /locus_tag="LLD04_000089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK5647056.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27852..28436
                     /locus_tag="LLD04_000090"
     CDS             27852..28436
                     /locus_tag="LLD04_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK5647057.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28451..29437
                     /locus_tag="LLD04_000091"
     CDS             28451..29437
                     /locus_tag="LLD04_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK5647058.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            29427..29738
                     /locus_tag="LLD04_000092"
     CDS             29427..29738
                     /locus_tag="LLD04_000092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK5647059.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29760..31541
                     /locus_tag="LLD04_000093"
     CDS             29760..31541
                     /locus_tag="LLD04_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK5647060.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31534..32910
                     /locus_tag="LLD04_000094"
     CDS             31534..32910
                     /locus_tag="LLD04_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK5647061.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32917..33552
                     /locus_tag="LLD04_000095"
     CDS             32917..33552
                     /locus_tag="LLD04_000095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK5647062.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33582..34937
                     /locus_tag="LLD04_000096"
     CDS             33582..34937
                     /locus_tag="LLD04_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK5647063.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35129..36523)
                     /locus_tag="LLD04_000097"
     CDS             complement(35129..36523)
                     /locus_tag="LLD04_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5647064.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36615..37586
                     /locus_tag="LLD04_000098"
     CDS             36615..37586
                     /locus_tag="LLD04_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK5647065.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37583..38365
                     /locus_tag="LLD04_000099"
     CDS             37583..38365
                     /locus_tag="LLD04_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK5647066.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38534..38905)
                     /locus_tag="LLD04_000100"
     CDS             complement(38534..38905)
                     /locus_tag="LLD04_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5647067.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39039..39482
                     /locus_tag="LLD04_000101"
     CDS             39039..39482
                     /locus_tag="LLD04_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK5647068.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39525..40334)
                     /gene="yidA"
                     /locus_tag="LLD04_000102"
     CDS             complement(39525..40334)
                     /gene="yidA"
                     /locus_tag="LLD04_000102"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK5647069.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40353..41720)
                     /locus_tag="LLD04_000103"
     CDS             complement(40353..41720)
                     /locus_tag="LLD04_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5647070.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41704..42534)
                     /locus_tag="LLD04_000104"
     CDS             complement(41704..42534)
                     /locus_tag="LLD04_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK5647071.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42697..43134
                     /locus_tag="LLD04_000105"
     CDS             42697..43134
                     /locus_tag="LLD04_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK5647072.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43210..43830
                     /locus_tag="LLD04_000106"
     CDS             43210..43830
                     /locus_tag="LLD04_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK5647073.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44019..44525
                     /locus_tag="LLD04_000107"
     CDS             44019..44525
                     /locus_tag="LLD04_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK5647074.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44542..45441
                     /locus_tag="LLD04_000108"
     CDS             44542..45441
                     /locus_tag="LLD04_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK5647075.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45528..46412
                     /locus_tag="LLD04_000109"
     CDS             45528..46412
                     /locus_tag="LLD04_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5647076.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46604..46675)
                     /locus_tag="LLD04_000110"
     tRNA            complement(46604..46675)
                     /locus_tag="LLD04_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46640..46642),aa:Arg,seq:ccg)
     gene            46735..48069
                     /gene="rpoN"
                     /locus_tag="LLD04_000111"
     CDS             46735..48069
                     /gene="rpoN"
                     /locus_tag="LLD04_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK5647077.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            48218..49309
                     /locus_tag="LLD04_000112"
     CDS             48218..49309
                     /locus_tag="LLD04_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK5647078.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49350..50351
                     /gene="gap"
                     /locus_tag="LLD04_000113"
     CDS             49350..50351
                     /gene="gap"
                     /locus_tag="LLD04_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5647079.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50461..51651
                     /locus_tag="LLD04_000114"
     CDS             50461..51651
                     /locus_tag="LLD04_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK5647080.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51755..52510
                     /locus_tag="LLD04_000115"
     CDS             51755..52510
                     /locus_tag="LLD04_000115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK5647081.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52626..53924
                     /gene="eno"
                     /locus_tag="LLD04_000116"
     CDS             52626..53924
                     /gene="eno"
                     /locus_tag="LLD04_000116"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK5647082.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            54324..55463
                     /locus_tag="LLD04_000117"
     CDS             54324..55463
                     /locus_tag="LLD04_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK5647083.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55530..56105)
                     /locus_tag="LLD04_000118"
     CDS             complement(55530..56105)
                     /locus_tag="LLD04_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5647084.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(56110..57084)
                     /locus_tag="LLD04_000119"
     CDS             complement(56110..57084)
                     /locus_tag="LLD04_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK5647085.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(57108..57152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57367..58335
                     /locus_tag="LLD04_000120"
     CDS             57367..58335
                     /locus_tag="LLD04_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5647086.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            58448..58726
                     /locus_tag="LLD04_000121"
     CDS             58448..58726
                     /locus_tag="LLD04_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5647087.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            58844..59080
                     /gene="secG"
                     /locus_tag="LLD04_000122"
     CDS             58844..59080
                     /gene="secG"
                     /locus_tag="LLD04_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK5647088.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            59183..59938
                     /locus_tag="LLD04_000123"
     CDS             59183..59938
                     /locus_tag="LLD04_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5647089.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59941..62298
                     /gene="rnr"
                     /locus_tag="LLD04_000124"
     CDS             59941..62298
                     /gene="rnr"
                     /locus_tag="LLD04_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK5647090.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            62320..62784
                     /gene="smpB"
                     /locus_tag="LLD04_000125"
     CDS             62320..62784
                     /gene="smpB"
                     /locus_tag="LLD04_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK5647091.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            63086..63805
                     /gene="atpB"
                     /locus_tag="LLD04_000126"
     CDS             63086..63805
                     /gene="atpB"
                     /locus_tag="LLD04_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK5647092.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63858..64073
                     /gene="atpE"
                     /locus_tag="LLD04_000127"
     CDS             63858..64073
                     /gene="atpE"
                     /locus_tag="LLD04_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK5647093.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            64164..64688
                     /gene="atpF"
                     /locus_tag="LLD04_000128"
     CDS             64164..64688
                     /gene="atpF"
                     /locus_tag="LLD04_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK5647094.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64675..65217
                     /locus_tag="LLD04_000129"
     CDS             64675..65217
                     /locus_tag="LLD04_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK5647095.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            65240..66796
                     /locus_tag="LLD04_000130"
     CDS             65240..66796
                     /locus_tag="LLD04_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK5647096.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66810..67712
                     /locus_tag="LLD04_000131"
     CDS             66810..67712
                     /locus_tag="LLD04_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK5647097.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67735..69141
                     /gene="atpD"
                     /locus_tag="LLD04_000132"
     CDS             67735..69141
                     /gene="atpD"
                     /locus_tag="LLD04_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK5647098.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            69155..69577
                     /locus_tag="LLD04_000133"
     CDS             69155..69577
                     /locus_tag="LLD04_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK5647099.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23164 a        12235 c        14828 g        19560 t
ORIGIN      
        1 tttgggtatc taaccatggt ggccgtcaat tagatggggg gcctgctgcg tttgattcgt
       61 tgcaatatgt ggcagaagca gtagataaac gtgtaccaat cgtgtttgac agcggtgtac
      121 gacgtggaca acatgttttc aaagcaatcg catcaggtgc cgatcttgtt gctattggca
      181 gacccgttat ttatggattg tctttaggag gaagtacagg agtccatcaa gtgtttgatt
      241 tctttaaaac agaattggaa atggtcatgc aattagctgg aacacaaaca gtagaagata
      301 tcaaaaaaat aaaattgcgt gagaatcgtt ttatttaaga aataaatgac gaaaaaaata
      361 ggagtgcggc agatgatgcc gtactcctat ttttatagtt caaggatgtc actaatagaa
      421 taatttgaaa cgaaaaaata aaattgaaat attttttcat acttaataaa aaaggaagaa
      481 tattcatgta aacgatttta ttgttttttt ctcttttata caaaaagggt cataatttca
      541 agtaagcgga tgaattcatg aggatttttt gcatactgtc tctactatac ttaggtcact
      601 aagaaaaagg agtgatttat tatgaaaaag aacatgattg ggacattatt actttcaaca
      661 cttcttctag gcggcttggc aacaccagct tttgctgagg gtcaagcgac ttcaaaagga
      721 gatattacct ttactgaacc aacaaatacc gttgagcctc ttaaccctac tgatccttca
      781 aaaccagtag agccagcaga tccggaaaat ccagcaactg gccagacagg ttctttaaca
      841 ttggatgtag ttcctgaatt gccatttggt acacacgaga ttgaaagcgg aacaaaaacg
      901 tatcaagtag acgcttcaaa aaacgataca ccttatctcc aagtatcaga tcgtcgaggt
      961 gtaggtgcag atggccaagc acaagggtgg aacgtgacag tttcggtttc agattttgta
     1021 aacggttcac aagttttgca aggcgctgaa ttagattttg gaacatccac tgttaaaagt
     1081 acgtcagata acgaatccac tggaccaact agtcaaactg taactgggtt gagtaaagca
     1141 agtgcagcga caccaatatt tacagcagca aaagatcaag gtctgggaac atggctttct
     1201 gtttatgatc cagcaaatat cacactgaaa gtgccgaaag cagcagctgg aacattcacg
     1261 gctgatttga cttggaacct cgtggctggc ccagtagcat agagtatgaa ataaattcaa
     1321 caaactgcgg tgaaaatact ataggtgaag gattttttac cgcagttgtt gttgaaagga
     1381 tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc gtttttttct attttaagtc
     1441 tatttttcat ttttccgtca atcagccaag caaaggattc agcaggagac tttggaatca
     1501 aaccggtatt tccagagaac caaatagata aagcaatcgg ttattttgat ttattagtgg
     1561 caccggaaca aaatcagaca ctagaagtaa ttatcagcaa ttcttcagat gaagaacgaa
     1621 catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg tggaacgatc gattatagtc
     1681 aaaaaaaccc aacgttagat gagacattgc cgtttgatgt ccgtgacgtt ttgctcattg
     1741 cgaaaaagga aatcaatgta tcagcacatg cagaaacaac tatacctata gaagtgaaaa
     1801 tcccagcaaa aagctttaag ggacgtgtat tagcgggtat tcatgtttca cctaaagaag
     1861 aagctgaaac agaaaatgct acagaaggag cacaaatcaa aaatcgtatt gcttataatc
     1921 tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc ggatctgaaa cttttatcag
     1981 gagatttgga cgaggtcaac gcaaagccaa ctgttcagct tcgtttccaa aatcctcagc
     2041 ctaggattat cagcaatttg atatttacga gcaagatctt ttatgaaaat cagctttata
     2101 ttgaaaatac gtctaatgaa tttttagttg caccaaattc gaattttcac ttgaatcttg
     2161 atttagctgg tgataaagca aaagccggcg attaccgcgc agaaatcata gcaaaatcag
     2221 gcgacagtaa tgaatggcga tttacacaaa actttacgat caaaaaagaa aaagcacaaa
     2281 aagtaaatga aaattccgtt tttgcagtac aagaacaatc tttcccatgg ctttatgcag
     2341 gattggttgc tgcagttcta gtgttatggt tgattttgct gtttttgtgg gtccacaaga
     2401 aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt tgaaaacatc gatcttattg
     2461 ggagggatta ttgcaagttt gctgttcttc agctggcaag agccagtcca agcagatacc
     2521 aaaagttcag gtacgattac attttatgat ctaagtccag ttccgcatcc aagtggttca
     2581 gtcaatggcg catcaaaaca gcagacacag ctaccgattt ataaagaatc taatcaacat
     2641 ggcgtattcc cggagacgaa tgaagaaaag atccgttttc tttatttgat cattggtata
     2701 gaattatggc ttcttgcgtg gttgttgtta tggagaagaa aggaaaagga ggatgaacat
     2761 gttgctacaa aataaatttt cattttttcg ctccttcttt tttacttcgc tttttgtttt
     2821 aagtatattt atcttttcac cttcggcaga agctgttgga gcaacgcctc ctgccatttc
     2881 ttatgatcca tacacgtatg aaaatatcgt acctccaaat ggtggacgta gtgcagtcaa
     2941 tggcgggaag cttgtccaac taactggggc taaccaattg cctaaattga ctgctactta
     3001 tttgaacgat ggttcaaatg atccttggaa aggtggcacg gtccgttttc attgggaaag
     3061 ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc actgcacaag gatttgcatt
     3121 tgtggcgatc atcaatttgc cggaacatgt atcaagcagt gatgtattaa aagcaattga
     3181 ctggaacaac gcctacttgt atattgccgg tgcccccatc aaggttacta gcggtagttt
     3241 attatcagca ggaagccata ccttgcgttt tgggatgggg tcgtgggata caaatactct
     3301 attggcagat attattgcta ttattacgcg aggcgaattc aacgtggacg atatcccgct
     3361 tcaatttgat gttacagcta atgtcgcgaa gatgacagaa aacggagata aatatgatac
     3421 gagtccaaac aaaattttga ccaagggtaa attacaacct tctatgtcta aaaaggccgt
     3481 tttttctgtt gatttttatg attcgaataa tattatccaa gatcgtatgt tcaattcgat
     3541 tttagctcca gctgcaggta gattttatcc taaattaact ggcggaaact tgaatccagg
     3601 agcatatgca ttttctgcaa gtgcagagat aaatacgtgg aatagttaca tctcaacaag
     3661 tgattcaaca ggaaaatata atacatttgc tgataccaca gaatacgtgg atggaaataa
     3721 ccagaacctt ccaggctcta ccaatagtcg gacattgacg atggggctga ctgaaggacg
     3781 ttttgatcag ttgccattta gtcgctttga tcgcgtagtc aactatttta caggaaaaaa
     3841 cgttacacaa ggagcaatac tgactcatac tccagcaagc acaagtggat tgaatcagac
     3901 agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga ttgctttctc ctgttgcttt
     3961 aaaggtattg gagcagtaca agctagacgg aacggtttta ccaacaaact taacagccga
     4021 tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa gtcacggcca attggtcagc
     4081 aactgaacta aaaaagggtt cgatccatta caggctttat tcgaaaggaa cccaaaaatt
     4141 agcgacagga tggacagatc aacttttcca aacgatccaa tcaaataacg ggggtgtaaa
     4201 tacagctact tctacactac ctgcattggc ttcagggcaa tattattttg attatcgctt
     4261 ggtggacgat gtacttacta acatgtatcc gtcttttgcg tacaaatggc agtctgaaca
     4321 agctggaatc accactcttc ctcaaataac agtggctaat tttccatctg tgagtgcaga
     4381 tagtcagcta aaaaacctta gcagacaacc tgagacagga gatcccctgt ctgctctatc
     4441 aggagatacg attcaagaaa atgtgacatt cacgttaaca aaatctgggg atgcgataac
     4501 cgacaaaaaa atcacgatct cacttcctga aaatactact tatatcaatg gttccttaaa
     4561 attaaatggt acaactatcg cagataccgg cattcaacaa ggagtttctg tcccagcaga
     4621 tctattgagc aaaataggag ataccattca tcttacttac aactatcaat taaatactgt
     4681 agatacttct gtacaatccg tcagcatact gacaaaggca gctgtacttt ccagcaatat
     4741 tacattagca gatggtgcta agcttcctaa cccagttgtc caaacatctg ctaaaacaat
     4801 tcttgtcccc aaacaagaat taacattagt taatgtacca gatgatttta catttggtaa
     4861 cgatctcccc aaaccgttga agacaagtta ttatgaagca aaaggcgatt tttcttttga
     4921 tgtgcgggat actcggcttc caagcacgtc tccttggcaa ctgacaggga cattgacttc
     4981 tttatttaag aatgatcaag gtcaagaatt aagcggaaca aaattatact tcaatcattc
     5041 tggatcaaaa caattgattc agcaaggtca aaacacgttg atttatgaga gtgatggaac
     5101 agctaaagga gaggttctcg ttgactttcc cgataccgat ggtttactct tagaggtaaa
     5161 tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc atggtcacat gggaactgac
     5221 agctgggcca accagctaag tgattagaat ttgtatggat ctgtgataac tgtcacagat
     5281 cctctttttc aaggatgaga aggaaacttc tgctgttaaa atcagaaatg cttggaggag
     5341 tggacttgga acttttcagt ttgcttgaaa aaaatgaaca acggcaaatc aaacttttcc
     5401 aacttttatt agaggaaaaa ggagagattt ccgttaataa cgtacttacc cgcttatcag
     5461 ttgatcgggc taccttgaaa gaagatctgt tttatttaca agctaacttg gaaaatatca
     5521 aacaattgga aattgttatc caagaaggga atattttatt aatcagacat ggaaatatag
     5581 cagcagtcga tgtttattat cattgctact tgcgctattc tacaaaatat cagatacttc
     5641 tttatctgtt ggaaaatggc tcatttgatc ggcagaaact tgtcatggaa cttaatatca
     5701 gttcggctac tttgtctcgc agagtgaagg aattaaatga ttctttaaat gagtttcatc
     5761 tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca aatttgttat ttttatttcc
     5821 aattattttg gtttggacgt ccttatcttg aaaacaaaaa agaatacagt gatttatcag
     5881 gaaacacttt tttagatctg ctgcaaaaag agtttagttt tactattaca gaagaaggac
     5941 gattgaaatt tgaaatatgg ctttccatta tgaagaagcg aatgcgctgt acgaaaaaat
     6001 ccaaaccaga ttatagcaat cttcctgttg gattgtttca aacgacacaa tttttattgg
     6061 acctccatac atttttgacc cgttacttct ttcaatatgc ctttccttgg aatcaaaatg
     6121 aaacgttggt tttttatctt ttcatgatca gtaactttac attagataca cataactcgc
     6181 atgtccagga gtttttgata agcgaaaaat acaaagacga cgtagtagct caattgaatc
     6241 gaacgtttaa acaatcgata gaagaatatt tctcttttta tgtttttcca gaagagattt
     6301 cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca ttttgcctat tttagaggat
     6361 ggatgaatat ttttggccaa gaaggattaa aacagcggtt aactgaagta gaagataaac
     6421 aattcctggt tttatgtgaa agattggtca gacaaacatt tgctattgtt gattttactg
     6481 aagtagacga ggaaaatgcc cgctttgaat tatttgctcg ctatgcatct attttgcgga
     6541 cgctgatcaa gcagttagaa ttacgtctgc aaattgctta tgatcttcct tatgaaaggc
     6601 taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt gctgaatacg atcaaatatg
     6661 atttagtcga atacgagaaa gaaaagcagt atgatatcat tttgactagc caattgaaag
     6721 agtacccgtc acagaagtcc gctaaagttt ttgtattcac tagcaacgaa tataattatg
     6781 atttccccta tttgaaccaa tttttaaaag aatgttattt agaaaaattg aatttaagga
     6841 tgaataaaac atgaaaatcg tgatcattgg tgcttcccat gcaggcatca cagctgccct
     6901 aaatctaaga aaacttcaac cgaaagcaga agttttactg atcgatgaag atcataaaga
     6961 tggactaggc tatgtttcaa atggaatcaa cctttattta aaaggaaaga tacgtagttt
     7021 agctgaagtt gcccataaca tgcggactct tcaagaaagt ggggcaaaac tgatcaccga
     7081 atggagagta actgaacttg atccagacaa acatcagctc atcctcgcat ccaaagaagg
     7141 aaaaaacgag accataacct atgataaatt aatcgtagca acaggttcct ctcctgtcac
     7201 cttgtataaa caaattgaag ctgaaaatgt ctacacctat aaaaatctag tgcaatctaa
     7261 gcaagtactt gccgctttaa aagaagcaaa agaagtagtg atcttcggtg ctggttatat
     7321 tggattggaa ttagcagatg ctttgagaaa taaaggatac attattcatc tagttgatta
     7381 tatgccgaat gttttatcaa gatatttcga taaggatatg atcaacagtt ttcaaaatca
     7441 gctgcaaact aagcaaatca atttttatcc aaatgaattc ttgatcgatt ggaaaaaatc
     7501 agaagagaaa gtagtatccg tacaacttct atctcaagca ataaaggcag atatggtcat
     7561 tttttctgcc caaactcgtc ctaatacgac attgttaaaa gaaaaagtgg ctttgtatga
     7621 ggatgagact gtgatagtca atgaatatct gcagacaagt gatccggaca tctatgcgat
     7681 tggagatatc gtcccagtca gttttgacaa aaataaacgg catctctttt tacctttagt
     7741 tactcgagct gtacatatgg caagagcagt agcgctaact ttgagtggtc agccaaccgc
     7801 ttacgacctc agacaaaaga taacagcagc agtcattata gactactttt taggaactgt
     7861 agggctgaca gaagatgaag cgccattctt ggaacaaaat accggttcgt gttcaggaga
     7921 atttgatcta tttccacaat acgatgaaga aaacaaaaca gtaaatgcaa agctgatcta
     7981 ccatccggat acattagaaa taattggcgg acagctgata agccaagaat tcttattatc
     8041 agatttgaac ctgcttgcgg atatcgtgaa acacaaaact acgattccac agctagcagt
     8101 tgaggatttt ggatttctgt ctgaacacac ctctcgtttt cactacctca atgagttagc
     8161 ttttaaagta ctagctaaga gagcaaatag caatagagta gataaaagac tgtgagaaat
     8221 atcacggtct tttattgtgt ggggatataa taaatattaa tcaatcgaga ataaaaaatt
     8281 catataatga aatagcggaa gaattttttg agcataatat tcgccaaaaa tttatcaagt
     8341 gataaaattg ttgtataaaa tgattatttc aggaggagga gttaaatgtt tttagcatta
     8401 aatgaaatta tgcattcaaa gttacgttat gcgttagttg caggggtgat gtttttgatc
     8461 gcctacctgg tatttttttt gactgggtta gcttatggct tagctcaaga taatcgaaca
     8521 gcagtcgaca agtgggaggc agattcgatt gtgctttcga aagatgcaaa ttcaaatcta
     8581 gggatgtcca tgattactaa gaagatagca gaagaagtcg aaggtgggaa agtggcgtat
     8641 cttgcccaga cacctggagt cgtgactagt aaagacagta cagaagaagg aaaaatcaat
     8701 gttagcttct tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt tgaaggaaaa
     8761 gcttttgata acgatgatga ggcagtaggc gatattagtt tgaaagaaga atatggtcta
     8821 gccgtaggag atacagttaa gttatctggt agtgacaaga cgtttaaact aactggcttt
     8881 acggatcatg ccaaattcaa tgtctcacca gttctctata caaccatcaa tgcttaccaa
     8941 gagatccgct tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc aatagttgtc
     9001 agaggtaaaa tcagtaattt acctgaagat ttagaacaga tcaaaatttc taaattcatc
     9061 aatgaattac ctggatataa tgcgcaagta ttgacttttg gatttatgat cggatttttg
     9121 atcgttattg cggctattgt gatcgggatt ttcatttatg tactgacgat gcaaaagatt
     9181 aatatctttg gtgtaatgaa agcacaaggg atcacaggag gatttattgc gcgttctgta
     9241 gtggcacaaa cattcatttt atcatttgtt ggtattcttc ttggtctatt aggaacagtg
     9301 ggaacatccc ttgttctacc tgatgcggtt ccattccaaa gcaattggct gttttttggc
     9361 gtcattagct tgttgatgct ggtagtagct gtgttaggcg cactattttc tgttcggaca
     9421 atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag gaggaagagc atgaaagcaa
     9481 tagaatttaa agaagtagaa aaaagtttcc aagatggcga ccaagtagtt aaagcattga
     9541 aagaaacaac catgagtatc gataaagggg aatttgttgc aatcatcggt ccttctggtt
     9601 caggtaaaag tacttttttg actttggcag gcggattgca gacgcctagc agtggctctg
     9661 tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag tgaacgcgtt aaactacgtt
     9721 ttaaagaaat tggctttatt ttgcaagctt ccaatctggt tccttttctg acagtaaaaa
     9781 agcagttatt attagtagat aagatcaaac atgaacatcg tcaaaaagaa gcaaaagaat
     9841 tatttgagca gcttggcgtg gataagttga tcaacaagta tccagaagaa ctatctggtg
     9901 gagaacggca aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc attattctag
     9961 cagatgaacc aactgctagt ttggattcag aaaaggcacg agaagtagtc gatatcttgg
    10021 caaaagaatc aaaagacaaa aataaagcaa cgatcatggt tactcacgat acacggctga
    10081 ttgacaaatg cgatagaata tttatgatgg aagatggtgt attgaaagaa aaagaataac
    10141 caaaaagcag gtggaaaaat ggtccatcga tacaacaaag atacacagca aaaaattttg
    10201 aaagaagcga ctgacttgtt tatgtccaag ggatatctcg gcacttcaac aagagagatc
    10261 gcacaaaaag ttgggatcac ccaacccaat ctctatcatt atttcggtga taaagaaaaa
    10321 ctatatacgg cagttttaga aaaccactta aaagatgttg gaaaagcttt gagagaaatc
    10381 gttcaaacaa gcgaaacggg atttcaagta accttgacaa aaatggcgca gtttttgatt
    10441 gagacacatc tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa tctttcaaag
    10501 gaaacaagag atcatctttt ctttctatgg aagaaaaatt atcgtgaacc ttttgaagaa
    10561 atcttttcag caaaccaatc tgtgctaaga aatgggattt caaaagaaat cgcagcaagg
    10621 cattttttct tagtgttggc accttacatc actcggtcga aaagacctgt ggaagaaccg
    10681 ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg tattaggaga agcgtaaagc
    10741 tgttatatag ggagaaaaat agttaggtga agaagcaaga tatgacgaag atcgttatat
    10801 cttgtttttt ttatataggt agtttttcat aaatacaaat ttgttattct ctcatgatta
    10861 tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga
    10921 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
    10981 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
    11041 tattttctac ggttatatct tttacagccc cttttttgcg tacttaagct gattttaagt
    11101 tttgaccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
    11161 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
    11221 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
    11281 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
    11341 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
    11401 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
    11461 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
    11521 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
    11581 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
    11641 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
    11701 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
    11761 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
    11821 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
    11881 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
    11941 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
    12001 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
    12061 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
    12121 caagcttttg aacaccgttt aaggaagagg ttgtgagcct gccgtttaac tccgcgtcac
    12181 aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
    12241 gagttggcat gagaacaccg tttattcgga acaagggact gtgacatgac ttttgtcaca
    12301 gttccattat ttttttagca gcttcaaagc tttttctgta ttggcaaaat gaagtttggc
    12361 atatttttca agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga
    12421 tttcgttcca gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc
    12481 ttccaaaaag ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc
    12541 tcctttagta acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt
    12601 tttaaaattt gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc
    12661 gatttttttt agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc
    12721 attgtatttt ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg
    12781 gatggttttt tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga
    12841 atctctaaca ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca
    12901 tactgttagc ggaccaaaat agctgccatt tccgacctca tcactaccta aaacggataa
    12961 ttgactgata ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg
    13021 tgcctccccc catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc
    13081 ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat
    13141 ctgtttattt aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt
    13201 tgctttgata atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg
    13261 caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat
    13321 catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat
    13381 ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa
    13441 acacgctaca aagcagtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag
    13501 catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta
    13561 tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat
    13621 caattgaaga aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa
    13681 aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac
    13741 gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg
    13801 gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat
    13861 taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc
    13921 atgctgagtt tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc
    13981 gggtttgctt tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag
    14041 catttttaca aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc
    14101 ccaacatcaa agttagcatt ttttgatcag gtatttgctt ttcacctaga tgaagctttt
    14161 tatgcaggaa ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg
    14221 ggggtttttg tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca
    14281 ggaggcatcc tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt
    14341 gcctttgttc cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt
    14401 atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt
    14461 ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta
    14521 agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat
    14581 aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga
    14641 caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag
    14701 aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg
    14761 cgaggcggga tccctatacc taaacttgaa aatatccgtc cccatatgaa acggatcgag
    14821 atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca
    14881 agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg
    14941 tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca
    15001 atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag
    15061 aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa
    15121 aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca
    15181 gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga
    15241 cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac
    15301 caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt
    15361 ccccatcgtc aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat
    15421 gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat
    15481 catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat
    15541 gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg
    15601 aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg
    15661 attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga
    15721 gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta
    15781 gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga
    15841 acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag
    15901 tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg atacaatcgg
    15961 gcaaatacga ttaatgccag catggagttc gatgtggata cactaagtcc aacgtatcgt
    16021 ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt
    16081 gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac
    16141 gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg
    16201 cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat
    16261 ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg
    16321 tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag
    16381 attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat
    16441 ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa
    16501 acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta
    16561 cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa
    16621 gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta
    16681 cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa
    16741 gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa
    16801 gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt
    16861 aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt
    16921 gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa
    16981 gtatagaaat gctatactaa tcatagattt taaaaatgga ggtctttgat atgtcacaag
    17041 taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt
    17101 gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag
    17161 aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc
    17221 ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag
    17281 tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt
    17341 tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt
    17401 ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc
    17461 aggctggatt gtttttttga cagacatgct ataataactt taattgaata tgaaggggag
    17521 taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt
    17581 tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa
    17641 aatcgtgtcc gctcatattt tactggcagc cataatacga aaacggaacg gcttgttagt
    17701 gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag
    17761 atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc
    17821 tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta
    17881 ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca
    17941 aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag
    18001 ccttgtcttt actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg
    18061 aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa
    18121 atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa
    18181 gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg
    18241 acaaatactg atttactgga tcgagatgta tttggttatg cgattgacaa aggatggatg
    18301 tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt
    18361 cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa
    18421 aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta
    18481 gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt
    18541 gaattggcga ataagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga
    18601 aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc
    18661 ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg
    18721 atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa
    18781 acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattca
    18841 cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga
    18901 caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct
    18961 ggcatggcaa aaaacgataa acataaaaca aatgagttgc tattcggtat agatttggct
    19021 gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa
    19081 gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc
    19141 cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc
    19201 aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg
    19261 aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta
    19321 caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt
    19381 ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg
    19441 agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc
    19501 gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg
    19561 gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa
    19621 gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg
    19681 gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa
    19741 cttagaggga aatcaagaaa agagattgaa ccaaaaatcg atttttggat ggaaaaattt
    19801 caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa
    19861 gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc
    19921 agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa
    19981 catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat
    20041 catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt
    20101 gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa
    20161 gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta
    20221 gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct
    20281 atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc
    20341 gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc
    20401 tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa
    20461 ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat
    20521 cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga
    20581 agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac
    20641 tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca
    20701 actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcaa gtcaattagg
    20761 catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt
    20821 caattttgat tcaaaaggaa aaatgacagt tggagaagat aactcgacag tccaatatgt
    20881 cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat
    20941 tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac
    21001 gactgcacaa aagcattttt acggaaaact aacaggtgtt ttacttgttg cattaacgca
    21061 aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat
    21121 ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta
    21181 ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg
    21241 ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc
    21301 tctaggcgga tatatgcttg gattgatact aggagcttca gatccaaaca atatcgttat
    21361 tcggatcact tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa
    21421 tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt
    21481 cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgtgt atagcgaagg
    21541 cggattatgg agttcattga aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc
    21601 ataaaatata gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg
    21661 tatagaccta tttctatttt tttattatat tggatatata tattcattga aaatagagca
    21721 taacaacttt ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt
    21781 agatttactt tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct
    21841 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga
    21901 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    21961 agaccttttc tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc
    22021 ttatccagaa tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat
    22081 ccatcgtttg atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg
    22141 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg
    22201 tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga
    22261 agaagaaaaa tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt
    22321 gcatatcgtg ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat
    22381 catggatttt tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc
    22441 gtattgcggc tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta
    22501 tttatttgaa ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca
    22561 tcaaacctat cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag
    22621 agagcaagaa gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga
    22681 acatcagttg ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa
    22741 aatcaactat gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac
    22801 ttgttttcaa acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt
    22861 gatcgatagt ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt
    22921 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg
    22981 tatacctgta gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg
    23041 aaaagaactg ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc
    23101 aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt
    23161 attcggcaag agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca
    23221 cgtcgaaaaa agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    23281 tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt
    23341 aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg
    23401 aggtctatcc aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa
    23461 ttcttacggt ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc
    23521 aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact
    23581 taatcggaat acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct
    23641 gttggattat cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa
    23701 aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa
    23761 aatattagaa gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca
    23821 cgacctcttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    23881 acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc
    23941 tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt
    24001 tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc
    24061 atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt
    24121 aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca
    24181 gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc
    24241 ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga
    24301 taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc
    24361 ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata
    24421 tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga
    24481 ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac
    24541 atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc
    24601 ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg
    24661 gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga
    24721 tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta
    24781 ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat
    24841 ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt
    24901 tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta
    24961 gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag
    25021 tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc
    25081 gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg
    25141 agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat
    25201 tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc
    25261 aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg
    25321 aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat
    25381 aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg
    25441 gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg
    25501 attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa
    25561 gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacat
    25621 caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa
    25681 ttagaagaag tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt
    25741 ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg
    25801 tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt
    25861 ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca
    25921 aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt
    25981 gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa
    26041 ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca
    26101 gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt
    26161 gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt
    26221 ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact
    26281 gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag
    26341 gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa
    26401 atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat
    26461 cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg
    26521 acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc
    26581 tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga
    26641 caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg
    26701 gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt
    26761 ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt
    26821 gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg
    26881 ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta
    26941 tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga
    27001 gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg
    27061 atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc
    27121 atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg
    27181 aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa
    27241 ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa
    27301 aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca
    27361 atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca
    27421 acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca
    27481 gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg
    27541 cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta
    27601 ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct
    27661 tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt
    27721 ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca
    27781 tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg
    27841 aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc
    27901 aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa
    27961 aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg
    28021 aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga
    28081 atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt
    28141 gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag
    28201 gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg
    28261 atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc
    28321 aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg
    28381 tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag
    28441 ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact
    28501 tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga
    28561 aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga
    28621 acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga
    28681 gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac
    28741 aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc
    28801 attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat
    28861 tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat
    28921 tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg
    28981 atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat
    29041 ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc
    29101 gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac
    29161 gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa
    29221 tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga
    29281 agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt
    29341 agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac
    29401 aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag
    29461 attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa
    29521 ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag
    29581 agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg
    29641 ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa
    29701 aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt
    29761 tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg
    29821 atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg
    29881 agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag
    29941 gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt
    30001 cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta
    30061 atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg
    30121 aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag
    30181 aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa
    30241 caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg
    30301 gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag
    30361 aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc
    30421 cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg
    30481 ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg
    30541 agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata
    30601 caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtgg
    30661 cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg
    30721 gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga
    30781 tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac
    30841 ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg
    30901 aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag
    30961 tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc
    31021 aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag
    31081 tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca
    31141 tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt
    31201 ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt
    31261 tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa
    31321 tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt
    31381 atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat
    31441 atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga
    31501 tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt
    31561 gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta
    31621 atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa
    31681 gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct
    31741 gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta
    31801 ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg
    31861 gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa
    31921 acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta
    31981 ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg
    32041 gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa
    32101 gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg
    32161 tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg
    32221 actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac
    32281 atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga
    32341 agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga
    32401 agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac
    32461 gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg
    32521 actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg
    32581 cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg
    32641 aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta
    32701 ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa
    32761 aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta
    32821 gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg
    32881 ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg
    32941 acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa
    33001 ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac
    33061 aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg
    33121 gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac
    33181 gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt
    33241 gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc
    33301 gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca
    33361 gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc
    33421 aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg
    33481 aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt
    33541 accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac
    33601 aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt
    33661 tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt
    33721 ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga
    33781 atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg
    33841 gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca
    33901 gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga
    33961 tgaagctcat gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg
    34021 ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt
    34081 tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca
    34141 atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt
    34201 taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc
    34261 attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct
    34321 ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa
    34381 acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc
    34441 atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg
    34501 ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt
    34561 cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag
    34621 tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga
    34681 ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg
    34741 tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa
    34801 tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg
    34861 tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa
    34921 gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa
    34981 aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt
    35041 gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata
    35101 agatttgatt cctttattta tcaataggtt ataattcata tttttttatt ttttctttta
    35161 cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga
    35221 atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag
    35281 caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat
    35341 agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg
    35401 ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga
    35461 tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt
    35521 gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac
    35581 aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag
    35641 gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa
    35701 cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt
    35761 gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc
    35821 tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga
    35881 tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg
    35941 ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac
    36001 cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg
    36061 ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa
    36121 gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa
    36181 aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga
    36241 tggactcaaa gacattggga gaattaaatg tgccacttcc aatattcgta tacagcaaac
    36301 ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat
    36361 cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat
    36421 cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg
    36481 gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa
    36541 attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga
    36601 ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg
    36661 aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc
    36721 aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga
    36781 tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg
    36841 ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa
    36901 acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg
    36961 tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg
    37021 gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta
    37081 cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa
    37141 aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag
    37201 cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa
    37261 gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta
    37321 catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa
    37381 gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta
    37441 cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga
    37501 ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca
    37561 aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga
    37621 tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg
    37681 tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa
    37741 aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca
    37801 cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat
    37861 taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat
    37921 ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat
    37981 tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga
    38041 aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat
    38101 aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc
    38161 aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta
    38221 ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg
    38281 ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt
    38341 ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag
    38401 agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat
    38461 gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa
    38521 tgccccatac taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa
    38581 agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag
    38641 ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc
    38701 cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat
    38761 ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc
    38821 tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc
    38881 gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata
    38941 acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa
    39001 atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg
    39061 aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag
    39121 atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa
    39181 ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa
    39241 ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc
    39301 gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag
    39361 gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga
    39421 aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact
    39481 gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag tacatatttt
    39541 ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct
    39601 ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat
    39661 aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt
    39721 gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag
    39781 ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct
    39841 gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc
    39901 atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta
    39961 taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga
    40021 ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga
    40081 cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg
    40141 taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga
    40201 cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt
    40261 acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact
    40321 agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta
    40381 cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag
    40441 gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct
    40501 aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa
    40561 atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg
    40621 tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa
    40681 gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt
    40741 ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat
    40801 tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag
    40861 cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct
    40921 tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa
    40981 acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata atcttccact
    41041 gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat
    41101 attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa
    41161 tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg
    41221 taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga
    41281 atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc
    41341 gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt
    41401 gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct
    41461 tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat
    41521 gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt
    41581 ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc
    41641 tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga
    41701 tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg
    41761 gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg
    41821 taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt
    41881 tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc
    41941 aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac
    42001 ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt
    42061 tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt
    42121 tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta
    42181 tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact
    42241 ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc
    42301 tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag
    42361 aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg
    42421 taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg
    42481 ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc
    42541 ggctcctttg tataaattca tattttctac aatattatac tctaaaatca aagagttgtt
    42601 ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg
    42661 aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg
    42721 atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt
    42781 gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt
    42841 caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta
    42901 cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta
    42961 gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg
    43021 catgttagtt tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc
    43081 atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa
    43141 aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac
    43201 gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc
    43261 gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct
    43321 aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa
    43381 ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc
    43441 ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa
    43501 gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat
    43561 gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa
    43621 gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat
    43681 tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga
    43741 aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag
    43801 gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag
    43861 agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt
    43921 gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc
    43981 ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga
    44041 aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc
    44101 aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga
    44161 tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt
    44221 ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa
    44281 aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa
    44341 cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa aaagagattc
    44401 tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga
    44461 atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag
    44521 aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa
    44581 acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt
    44641 atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga
    44701 gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga
    44761 agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc
    44821 tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag
    44881 aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat
    44941 tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga
    45001 gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa
    45061 acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc
    45121 cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa
    45181 gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag
    45241 caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt
    45301 tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt
    45361 tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg
    45421 gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt
    45481 aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt
    45541 atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg
    45601 cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt
    45661 tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag
    45721 aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca
    45781 gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac
    45841 caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca
    45901 cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg
    45961 aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg
    46021 cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca
    46081 aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca
    46141 tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg
    46201 atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg
    46261 atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag
    46321 ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt
    46381 atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca
    46441 cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat
    46501 acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat
    46561 aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc
    46621 gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca
    46681 ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa
    46741 tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag
    46801 ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag
    46861 aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac
    46921 ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct
    46981 gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat
    47041 acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg
    47101 acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat
    47161 gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa
    47221 tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg
    47281 gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct
    47341 gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct
    47401 ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg
    47461 gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt
    47521 caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta
    47581 aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca
    47641 attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa
    47701 cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc
    47761 acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta
    47821 aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga agacctttca
    47881 gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg
    47941 ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga
    48001 acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat
    48061 gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg
    48121 aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat
    48181 gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt
    48241 ggacgtaaag tgtccaacta aggagatcgc tatgttggat gaaataaaaa tgattgaatc
    48301 tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta
    48361 ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt
    48421 attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg
    48481 tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca
    48541 gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg
    48601 tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc
    48661 gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg
    48721 aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca
    48781 taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc
    48841 tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga
    48901 acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt
    48961 aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc
    49021 agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc
    49081 ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat
    49141 cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa
    49201 atcaaaagcc aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat
    49261 cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt
    49321 ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg
    49381 gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag
    49441 caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc
    49501 aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag
    49561 aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag
    49621 atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa
    49681 cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg
    49741 tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat
    49801 gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg
    49861 aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc
    49921 atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa
    49981 caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag
    50041 ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa
    50101 aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg
    50161 gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat
    50221 ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg
    50281 cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg
    50341 ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg
    50401 gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa
    50461 atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt
    50521 gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca
    50581 ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt
    50641 ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt
    50701 ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta
    50761 gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt
    50821 gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct
    50881 tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct
    50941 aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa
    51001 atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt
    51061 ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa
    51121 atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt
    51181 aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc
    51241 gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa
    51301 acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca
    51361 tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct
    51421 atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc
    51481 gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa
    51541 caattaggat ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa
    51601 ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag
    51661 tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag
    51721 ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa
    51781 ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa
    51841 aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga
    51901 attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga
    51961 aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca
    52021 ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat
    52081 caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgtggcga
    52141 atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc
    52201 tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat
    52261 ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt
    52321 tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca
    52381 atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga
    52441 cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc
    52501 tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa
    52561 attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac
    52621 aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc
    52681 caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt
    52741 caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt
    52801 atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa
    52861 tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg
    52921 gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg
    52981 cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca
    53041 caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact
    53101 caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc
    53161 tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag
    53221 caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt
    53281 ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc
    53341 ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag
    53401 ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag
    53461 tttctaaata tccaatcatc tcaatcgaag acggattaga cgaaaacgac tgggacggat
    53521 tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg
    53581 taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta
    53641 tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag
    53701 aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct
    53761 ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg
    53821 accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag
    53881 aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag
    53941 atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc
    54001 tctacttgct gaaatatcag caggtagaga gttttttttg caactagctt ctgtatatga
    54061 tgtttacaaa gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact
    54121 agcatcatat aagagctttt aaggatagaa cagtagaata ggaatctatg agcaagtcct
    54181 ctcgtataaa taaaatagga gggaagtagc agcatatttt tcataaagtg gctctctttc
    54241 ttcattttta ctttggttgc tcctatttga aaattcgagc acactcaaat acaataaaaa
    54301 gaaatggcaa gggagaggat tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga
    54361 tcatccgaat taaatcagtg aatggaaacg aaggtgaagt agcagcctat ttgaataagc
    54421 ttttggcaaa gcatgacata acaggagaga ttgtttctta cagagatggt agagataatt
    54481 tgattgctcg ttaccaaaag ggccaatcag gaaaagtgtt aggcctatct gggcatatgg
    54541 atgtcgtggc ggctggtgat gaatcgtctt ggacctatgc accctttgca gcagaaatcc
    54601 atgggaaccg tttgtacgga cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg
    54661 tgattgccat gatcgaactg aaagagtcag gaaaaccatt taacggaaca gtcaaattat
    54721 tagccactgt cggagaagaa gtgggtgaac taggtgggga acagctcacg aaagcaggct
    54781 atgtagatga cttagatgcc ttgatcatcg gagagccaac taattacagt ttgatgtata
    54841 cgcacatggg atcaatcaac tatacagtca cctcgcacgg aaaagaagcg catagctcta
    54901 tgccagacca aggttataat gcaatcaatc acctaaatga attcattacg aaagcaaatg
    54961 ctgaaatgaa tcacctcgct gaaacaatcg agaatcctgt cctaggaaaa acaatccata
    55021 atgttacatt gatcagtggc gggaatcaag tcaacagcat cccaagccat gcgcagctcc
    55081 aagggaacat tcgatcgatt ccagaatacc caaatgataa aatcattgca ttgctgcaat
    55141 caatcgtcaa tgaattgaat caagagacag actatcattt ggaattgatg atcgattaca
    55201 acaaaatacc tgtcaaagct gatccagatt ctcctttgat tcattgcatt cagcaacaat
    55261 tcagccagcc attaccttta gttggagcgg cagcaacaac ggatgcagcc gaatttacga
    55321 aagctaacca ttccttcgac tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc
    55381 aagtagatga gtatgttgaa atcgataatt atttggatat gattgaaaaa tatcaaggaa
    55441 ttattttatc ctacttagcg taagattttt ttaataaatg aaaaaacaga gccagattag
    55501 cattattttg ctaatccagc tctgtttatt cagcctattt ttctttttga atatatcaag
    55561 cgttgtggca acatagtgat gactaatgaa acaacgacga tagacaacaa acgatcaagg
    55621 tagtctgtac ctgcctgtac caagatcaca cttgttgtct gacttaaacc taacccatgt
    55681 aatagctgaa cgatcatact ggatcctgag gaggtgatcc cgccaaataa ctgaacggta
    55741 atgatcgaag aaacgatcgt tccaggtacc gacaaaatca aagcgagtaa taacagtttc
    55801 aagctgtttt tatctttgcc gagatatctt ggcaaaatca atccagcaag caagcctgtg
    55861 agaatttgga ttggcgaata atacagagaa aaaatatctg tagtcactcc acttaaaata
    55921 ccgctggaca atcctgtcag cattccaggt acaggcccaa gcaatgcacc agcaaaaatc
    55981 gttccgatag aatcgagata gattggcaaa cgaagccata aagcaatcgt tcctccaata
    56041 taattgatcc caatacacaa agccatcact gtaagtacgc gagttgccat tttgttttta
    56101 ttcattttgt tagcccatct ttaacctttc caaatctttc ataatcgttt cttcttgtgc
    56161 atttaaaaga acggttaaaa actttcggaa aaaaagacga gtatctactt ttgtcataat
    56221 tttactgtta gcaggtttct gccaaaattc ataacgatca accaatgttt gtccacgact
    56281 tattccttga gtttccacag cagtgaagct tttgaaacct tcacagatgg ccccctcgat
    56341 aaaataagca acagctaacg gatcattgat gacacatcct aaaattcttt cttgtttcca
    56401 gtggaagtca aaataaaaac gagtgatcgc ttttagatac tctccctctt caggattcat
    56461 ttgacagcag tattcgagga tagtcggtgt caagacgatt tctcttgtca catctaaccc
    56521 aaccatttct atcgtttgtt tgagattctc gaatacataa gaagctgctt caggatcaca
    56581 ccagtaattg tattcagcaa ctggcgaaca gttaccatgg cttttgtaag ttccacccat
    56641 cgagacaaaa cgatccatat gtttcccaat atttgggttg actttcaaag caaaagcgat
    56701 attggtcaaa ggacccaaag caatgactga tgtatttgtc ttttctttga aataatcagc
    56761 caaaaaatca acggcatgga gaggttctgc ctgttttctc aagatcatcg ggaaatttgt
    56821 ttcccctaaa ccatccatac catgagtgtc ttgtgcactg atgaaaggtt gcttcaaagg
    56881 tttttccatt ccttgataaa caggaatgtc taaacgatca cagcgttcca ggcatttcag
    56941 cacattctgc gtacctatat ctgcagggac attcccacaa actacggtaa tagcaactac
    57001 ttcgatatca ggggatttga cagctaatga taaagctaaa gtgtcgtcga tccctggatc
    57061 gcaatcaata attacttttc gcattatttc caatctcctt tctttagaga tacttagttt
    57121 tttattctgg gaagtttccg aaccagtcca agacgagcgt cttgattata atgcacgaaa
    57181 ttagtatact ggcttttatt gcgaatgtaa atgctctctt acaagtaaat ctattgagga
    57241 gaactttaag taaatgatac aatgaaaaag aaatggaatt tacgtgtgcc gaaaataaag
    57301 aatcctatgg atcggtacat tggataatag ttagattata gtttggcaga aagcggcggg
    57361 tgtacaatgg ttatttttta tttcattctt attctgataa ttatagggat cagttaccat
    57421 tttctttctt atttttatgc aaagaaatta gtaagagcgg gagtacagag aggatatgac
    57481 tggtatgaag acactggtca tcgtttgatg gaccctgctg cagtcacgtc agtagaaaaa
    57541 aaacgacaaa aattagaaga agagtgtgaa agcttttggg agcagggaac acccaaaaca
    57601 atcaaaagtt atgacgggtt gaaactagct ggtcagatgt tcatccatcc aaataagcag
    57661 aataaatggg tcatttgcgt acatgattac cgaagcacag ggaaaagaga catgtctcat
    57721 ataggtaaaa gatatgcaga gaaaggattc aacgttcttg tacctgattt acgtgcgcac
    57781 ggagaaagtg aaggagaaat catcggaatg ggatggctag atcggctaga tttaattgcc
    57841 tggatccaac tcatcttaga tgaacagccg aatgcgtcca ttattttaca cggcggatca
    57901 atgggagctt ctacgatcat gatggcaagt ggtgaaaaac ttccatcagc ggtcaaagga
    57961 tttattttag acagcggata tgtttctgtt tatgcagaat tccgatatat gttaagtaaa
    58021 atcactgtct ttcctaaaaa aatggtcatg agatatgcga atcattatgc ccagaaatac
    58081 gccggttact ccttaaagca agcatcagct acgagacagc ttggcagcaa ccatctgcct
    58141 ttattggtca ttcatggtga aagagatcat tttgtcccta cagaagcagc gtacacgatt
    58201 caaaatgcta cggcagggga taaagcactg ctgcttgttc cagaagcaga acacttggaa
    58261 gcatcgatga aagatccgaa aacctattgg acagtgattt tttcatttat tgaacaaaga
    58321 ataacgatga attaattttc tcttcttttt tgtaagtaat tcgaggataa ataattgttt
    58381 atagatcagg agactgctaa aataaggtag gaataaaaaa atatcagtta tttgaggagt
    58441 gcggcatatg gaaatcaaag aagaaaaaaa tcgtcttgct ttattcaatg atgaacaaca
    58501 agagatcgga gaaatgacat ggtcagatgc gggcccagac atcatgatca ttgaccatac
    58561 atttgtagat ccagcatatc gtggtcaaaa gttagctgaa aagctagtgt atactggagt
    58621 agaattagca agacgagacg ggaaaaagat cattccttta tgcccttatg caaaaaaaga
    58681 gtttgaaaag aaaccagaat atcaagacgt cttacgtcag tcataagaat aacagctgaa
    58741 agttttgaac tttcagctgt tattccattt atatagagtt gtcacggact tttatctatg
    58801 ataaagttaa agagacgata tcttttggga aggaggctgc gatatgtata atttaatttt
    58861 gggaatcgtg atcgttttat cgattgttat ggtcattgcg attatgatgc agccaagcaa
    58921 gcaaaatagt gcagcgagtg catttaccgg cggtgcggat caactattcg gcaaacaaaa
    58981 agctcgcggc tttgaagctg tcatgcagcg atcaactgca gttatgggag ctatctggat
    59041 gatcttatta tttgtcctag catatttatc ttcgaagtag ttgaataagc ctctcgcaaa
    59101 acggcgggcg gcttttttta ttgcaaagag aacgcttaag aagaagttat gaaagaaact
    59161 atggtaaaat gaggcgaaag aggtgagacg agtgagacaa ttaccaaaac ccctttatgc
    59221 aaaacatgga aagcgagcgg ttttgctgct tcacgcttac tcaggaagtc caaacgatgt
    59281 tcgaatgctc gcacgatttt tggaaaaagc agattataca gtttatgcgc cgctttttaa
    59341 aggacacgga acaatggagc catatgatat tttgcaagaa aaagcagaaa gctggtgggc
    59401 agacactaaa aaagccatcc attttttaca gtctgagcag ttttctgata ttgccgtatt
    59461 aggattatca atgggcggca tctttgctgt acgagcacta gaagaagaat cagtgattgg
    59521 cggcggtttt ttctgttcgc ctttgtctcc tgtaaaaaca aacgtgcctg aaaactttga
    59581 aaaatacgta cgtcaagtgc tgaagacagc aggtaaatca gagaaagaaa tcaatgaaaa
    59641 agcagttgct taccgctcgt tagctgaaca acaattaatg gatatacaag atcaggcagc
    59701 aatcgtagaa agtaggttgt cagatatcca acagccgata tttttagctc aagctgggaa
    59761 agatgaaatg atcgacccta atggcgtatt tgaaactgca cgcaagttga gccgtcagcg
    59821 agtgacactt cgatggtatc ctgaaagtgg tcatgtgatt acagttggta cagcaagacg
    59881 cgaattagaa aaagacgtct tggaattttt agaaaaatta ccttggaatg aggaataaat
    59941 atgacaaaag aaacgataaa agagaaaatt ctctttttca tggaaagcag tcgcaaacgc
    60001 agtttttcta tggaagaaat cgctgaaggt ttagggttcc aaaaaagcga tgattttaaa
    60061 ttactagtac aaacgattgc tcaaatggaa agagaacaga gtatagtttt caataaaaaa
    60121 ggaaaagtga aacttccttt aaaaccaatc ttgatcgaag gaacatttcg agcaaatgaa
    60181 cgaggatttg gatttgtaac gattgatccc gaagaagatg acatctatat cccaaaagaa
    60241 gcgactggtt ttgcaatgga tggagacacc gtagcaatcg atatcatgaa gcgagcaaat
    60301 acagatatgg atcgcggagc agaggggaaa gtagtcgaga ttcgagaccg ggcgactact
    60361 cagttagcag gggaattcgt tgcttattca gaagaagaaa tacaagaaac ggatctttac
    60421 ggcgttgtta tcccgaaaga tagaaagctc aaccaattta aagtatatgc agcagcagag
    60481 ggagttcgtc cagttgacgg aagtattgtg ctgatcgaac tgacccatta ccctgaaaaa
    60541 gggtatgcga ctagtttaga agggataatc aagcaagtga ttggtcataa gaatgaccct
    60601 ggcatggata tcctctcaat tgtcgtggct aatggtatcc ctacgaaatt tcctgatgat
    60661 gtattgtctg aagcagatga agttccagat caaatcagcg aaaaggatat aactggacgt
    60721 agagatttaa gaaatcagct cattgttacg attgatggag aagatgcaaa agatctagat
    60781 gatgcagtta cggttaaaaa attagataac ggtaattatt ttcttggagt acacattgca
    60841 gatgtttctt actatgtgac acaaggcagt caattggaca gagaagcata tgaaagaggg
    60901 acaagtgtat atttgactga tcgagtgatt ccgatgatcc cgcaacgttt atcgaatggt
    60961 atctgttcac tgaatcccca tgttccaagg ctgaccatga gttgcgaaat ggagattgat
    61021 ccaaatggac aagtagtctc tcatgatatt ttcccaagtg tgatccaaac aacagaacgg
    61081 atgacctaca cagctgtcaa tcaaatctta gaagatcaag acgaagaaac gatgcaacgc
    61141 tatgctcagc tcgtacctat gttccaagaa atgggtgaat tgcatcgaat ccttgaaacg
    61201 atgcgaatca aacgtggcgc gatttctttt gaggatcgcg aagcaaaaat ccttgttgat
    61261 gctgaaggac acccaaatga tatccagcta agaagcagag gaattggtga acgcttgatc
    61321 gaatctttca tgcttgcggc caatgaaacg gtagctgaac attataataa attaaatctg
    61381 ccgtttattt accgaatcca tgaacagcca aaagaagaaa aaatgcagcg cttctttgat
    61441 tttgcttcag ctttgggcat tctggtccga ggaacgaaag gaacgatcac accaaaagat
    61501 ttacagcatg tgatcgaagg agtagaagac aaaccagaag ctgcagttat caatactatg
    61561 ttgctaagaa gcatgcaaca agcacgctat tcagaagaca attttgggca ttatggctta
    61621 gcggcagatt actacacaca ctttacatct cctattcgac gctatcccga tctaatcgtc
    61681 catcgtctga tccgttctta cagtgaagat aaaagtgaaa gcaatcaaag ttcgtgggct
    61741 acggaacttc ctgaaattgc agatcatagt tctaagatgg aacgtcgtgc agtagaaaca
    61801 gaacgtgaag tagattccat gaagaaagca gaattcatga tggacaaagt gggggaagaa
    61861 ttcgatggga tcatcagctc tgttgtgaaa ttcggcctgt ttatcgaact gccgaataca
    61921 gtagagggat tgatccatat caatgaattg aaacaggact acttccattt tgttgaaaat
    61981 catttagctc ttgtaggtga aagaactggt ctgactttta aaatcggaca gaaagtgcga
    62041 gtaaaagtag tcaaagctga tccagatgaa cgagcagtag attttgaact tgtttccgca
    62101 gaagaggtta cagcgttgga acggccaaaa acacgaaatg gaaaatcagg aaaacgcgaa
    62161 ggaaaacaag caggaaaacg cccagaaaga aatggcaaga agaacaaacg atatagcgat
    62221 agtaagcaaa cgttcggtaa gaaaaaaggg aagaagccat tttacaaagg tgttaaaaag
    62281 aagaaaaaga aaaaataaaa tgagttggag gaaacagcta tgccaaaagg cgagggaaaa
    62341 ttaattgcac aaaacaagaa agctcgccat gattattcga tcatcgacac gatggaagca
    62401 gggatggtct tgcaaggaac cgagatcaag tcgatacgaa acagccggat caatctaaaa
    62461 gatggattta ttcgcgtccg caacggagaa gctttcttgc ataatgttca tatcagtcct
    62521 tatgaacaag gaaatatttt taatcatgat ccgttgcgca cgagaaagtt attattacac
    62581 aaaaaacaaa tcatccggct tgaaaatgaa ttgaaaaata ctggaatcac tgttgttcct
    62641 ttaaaagtct atattcgtaa cggctatgcc aaggtattga ttggtctggc gaaagggaaa
    62701 aaatcttatg ataaacggga agatttgaaa cgaaaagata tcgatcgaca aattgatcga
    62761 acattaaaaa atttctctag ataatttcat tatccttttt cgcatattcg gtgcttttgc
    62821 ttttacttaa tgataaaatt tgttagcatt taatctgata aaacatgatt atcataggtt
    62881 ttttcttaga tcaggagcac cgtgtataaa gaaaaattcg cttcgttttt gactgaaata
    62941 aaaaagtgtc taattttcat gaaaatgttt tgaattttct gtttcatggt ggtatgatac
    63001 cgtgtggtta gaaaccgatt ggaaaaaacc agatacgcac atgaattgat tttatgtgaa
    63061 tttcggggaa aagaggtgaa atggattgga tgaacgctcg ctaacgttcc acattggacc
    63121 aatttggttt gatggtacag tttgtataat ggtgttactg acctgtttga ttgttttctt
    63181 cttagcttac ttttttacaa gaaatcttaa gttgaaacca acaggcaaac aaaatgcgat
    63241 agaatgggtc attgatttta gtcgaggaat cgtaacagat aatctgccta gaaaagaatt
    63301 gagcaatttc catttgttgg catttacatt gtttttattt gtttttgttg caaacaacat
    63361 cggtttagta acaaaaatcg ttttacctaa cgaaacaaca ctttggaaaa gcccgacagc
    63421 agacccgttt gttactttaa ctttggcatt tataatgatc ttacttactc acttatttgg
    63481 ggtgaaaaag ttaggcttca aaggatattt cgtaaattcg ttcttgaaac catatagttt
    63541 tatgtttccg atgaaactga ttgaagaatt tacaaactta ttaacacttg ctttacgtct
    63601 ttatgggaat atttatgcgg gggaagtttt gctaaccttg attgcaaaaa tggtaataag
    63661 cttaggctgg ttctcattgc cgttagcaat tccattagag atggtctgga tcgcattctc
    63721 cttgtttatc ggaagcatcc aagcatttgt cttcgttact ttatctatgg tttatatgag
    63781 ccataagatt gaagtggaag aataatattc gttttaaaaa caaactacta tttatctaat
    63841 ttaggaggaa aacaattatg aattatatcg cagcagcaat cgcaatcatg ggagcagcaa
    63901 tcggagccgg ttatggtaac ggtcaagtta tctctaaaac aatcgaatca atggctcgcc
    63961 aacctgaaat gtctggtcaa ttccgtacaa caatgtttat cggggtagct ctagtcgaag
    64021 ctgttcctat cttaggggta gttgtcgcct tgattctagt ttttggtgtt taattgaatc
    64081 agtattaagg cagaaacaat ttggtttgtt actgcttttt tacatgaaaa ctagaagtac
    64141 ttcaaagaaa ggaaggctcg cctatgctga atcaattggc aattgcagaa gttggcaatg
    64201 cgatgttagg aaatgtcatc gttgtcagcg gctcattttt gatactactg gctctcttga
    64261 agcattttgc ttggggacca atcagcgata ttttgaaaaa acgtgaagac aagatcgcca
    64321 atgatttgga ttctgcagaa caatctcgca tcaactcagc gaaaatggaa caagaacgcg
    64381 aacaacaatt gttagcctct cgttctgatg cagctgatat catcaaaaat gcgaaagaaa
    64441 gtggagaatt aagccgccaa aatattttga aggatgctca agaagaagca gctcgtctaa
    64501 aaagtaaagc ccaagctgat atcactgtag aacgtgattc agcgctgaac tctgtaaaag
    64561 acgatgttgc agaactatct cttcaaatcg cggaaaaaat cttgaacaaa gaattatctc
    64621 cagaaatgca cgaatcatta atcaatcaat atatcgaagg tctaggttct tcaaatgaaa
    64681 ctagataaat atacagtagg aagacgttac gggaaagctt tatttgaact ggcgatcgat
    64741 tcaaataaag cagaagaaat ttatcaagat ctcgtaagct tacgtcaaat ctatgaacaa
    64801 gtacctggat taggtaatat gttaagtgat gttcgtctag aacctcatga aaaacgagaa
    64861 atcatggaca agcttgttag tggctatgat ggaatcgtta aaaacttttt agaagttgtt
    64921 tatgcgtata accgaatgga tgatcttttg ttcatgatcg aagaatatga acgtcgatat
    64981 gatgaacatc aaggtttgct gttgggaagt gtcacaacag cagttccact ttcagacgaa
    65041 caacgaatac aattagaaaa gaatgttgca aaaacaatga actatcaatc agtcgaatta
    65101 aaacaaatcg ttgattcttc tattattggt ggcgcgattg ttgaagctaa ccatcgagta
    65161 atcgacggaa gcattcgcac gcaattagaa aatatgcgta atcaattaaa tagatagtac
    65221 aaagaggtag gtgaatcgaa tggccatcaa agcagaagaa attagtgcct tgattaaaga
    65281 acaaattgaa aattatcaaa acgtgctttc agtcgaagaa atcggtaccg taacttatgt
    65341 aggtgacggg atcgctcgtg cacatggttt agaaaacgcg atgagtggtg agttgcttga
    65401 attttcaaat ggctcatacg gaatggcgca aaacttagaa tcaaatgatg taggtatcat
    65461 tatccttggc gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggaaagat
    65521 catggaagtt ccagttggag aggccttgat tggacgggta gttaatccgc taggtcagcc
    65581 aattgacgga ctaggtgaaa tcgttactga caaagctcgt ccagtagaag caatggcacc
    65641 aggcgttatg caacgtaaat ctgttaacga accaatgcaa acaggtctaa aagcaatcga
    65701 cgctcttgta ccaatcggac gcggccaacg tgaattagtt atcggtgacc gtaaaacagg
    65761 gaaaacttcc attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat
    65821 ctatgtagca atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa
    65881 atatggcgca atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcgccgtt
    65941 gctttatatc gcaccatatg ctggtactgc aatgggtgaa gagttcatgt acaacgggaa
    66001 acatgtcttg atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc
    66061 cttgctgctt cgccgtccac caggtcgtga agcttatcca ggggatgttt tctacttaca
    66121 ctcacgctta ttggaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac
    66181 tgccttacca tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt
    66241 tatttcgatc actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg
    66301 tccagcggtt gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc
    66361 aatgaaaaaa gttgctggga ctttacgtct agacttagca agttaccgtg aattagaagc
    66421 atttacacaa ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg
    66481 ccgtacagta gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt
    66541 agtgattttg tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt
    66601 agacttcgaa catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac
    66661 aatccgtaca acaaaagatc ttccagaaga agaagcgttg aacagtgcga ttcaggaata
    66721 taaagacatg ttcttagcta caaaaggaaa taattcctct acagaagata aattgaaatc
    66781 cattcaaaat gcatagtgag gtgagatgaa tgggtgcttc attaaatgaa atcaagacac
    66841 gtatcgcttc aacgaaaaaa acgagccaaa tcacgcgggc aatgcagatg gtgtctgctt
    66901 ctaagttgac aaaatctgaa gcttcttcac agaagtttca ggtttatgca acaaaagtaa
    66961 gagaaatcgt tacgcatttg actgctactc agttaaatga tattgcctca gaaaatccta
    67021 gaggggatat caattacaac agcatgttgc tcagccgtcc tgttaagaaa acgggttata
    67081 tcgtcatcac tgcagatggc ggtttagtcg gtggatataa cagctcgata ttgaaacaaa
    67141 cgatgtctat tttagaagaa gaccaccaat cacccgatga ttacgtgatg atcgcaattg
    67201 gcggaactgg tgcagacttc tttaaagcac gcggtatcaa tctagcatat gagctgcgca
    67261 atctatctga tcagccaagc tttgatgaag tccgcaaaat cgtaaatatg gcaacaacta
    67321 tgtaccaaaa cgaagtattc gatgaattat acgtatgtta taaccatcat atcaattcgc
    67381 taactagtca gtttcgtgta gaaaaaatgc tgccaatctc agacttagat ccagaagaag
    67441 caactacttt cgaacaagaa tatatttttg aaccttcaaa agaagaaatc ttgtctcagt
    67501 tattgcctca gtatgctgaa agtttgatct acggtgcgat cgttgacgcc aaaacagcag
    67561 aacatgctgc tggtatgaca gcaatgaaaa cagcaactga taatgctgct aatatcattg
    67621 atgagctgac ggtttcttac aaccgtgcaa gacaaggggc tattacccaa gaaattaccg
    67681 aaattgttgc cggagcttcg gcactagaat agtattgaga ttggaggaaa aaagatgagt
    67741 tcaggcaaga ttgttcaagt aatcggtccc gttgtcgacg tggaattttc tttagaccaa
    67801 tccttaccag atatcaacaa cgcattagtt gtttataaaa atgacgaaaa taaatcaaaa
    67861 gttgttcttg aagcagcgtt agaattaggg gacggagtga tccggaccat tgcgatggaa
    67921 tcaacagatg gtttacaaag aggaatggaa gtcattgata caggcaaagc aatctctgtt
    67981 cctgtaggta aagaaacatt aggtcgcgta ttcaacgtac taggagatac gatcgattta
    68041 gaaacacctt tcccagaaga tgcggaaaga agcgaaattc ataaaaaagc accagccttt
    68101 gacgaattaa gtacaagtac agaaattttg gaaacaggga tcaaagttat cgatttgctt
    68161 gccccatatt taaaaggtgg gaaagtcgga ctattcggtg gtgccggtgt tggtaaaacc
    68221 gtactgatcc aagaactgat ccataatatc gcccaagaac atggtggtat ttctgtattt
    68281 accggtgtag gtgaacgtac tcgtgaaggt aatgacttgt attatgaaat gaaagattca
    68341 ggagttatcg aaaaaacagc catggtgttc ggacaaatga acgaaccacc aggtgcacgt
    68401 atgcgtgttg ctttgactgg tttgacgatt gcggaatatt tccgtgatgt agaaggtcaa
    68461 gatgtactgt tgtttatcga caacattttc cgtttcactc aagctggatc tgaagtatca
    68521 gccttgttag gacggatgcc ttctgcggtt ggttatcaac caacattggc aacagaaatg
    68581 ggtcaattgc aagaacgtat cacatctacg aaaaaaggtt cgatcacatc gatccaagca
    68641 atctatgtgc cagccgatga ctacactgac ccggcgccgg caacagcatt cgcccatttg
    68701 gatgcgacaa ccaacttgga acgtaaatta acagaacaag ggatttatcc agcggtagat
    68761 ccattggctt cttcttcaag tgctttagca cctgaaatcg tgggagaaga acattataaa
    68821 gtagcaacag aagtccaaca cgtattgcaa cgatatcgtg aattacaaga tatcattgct
    68881 atcttaggta tggatgaatt atccgatcaa gaaaaagtgt tagtttctcg tgcccgcaga
    68941 gtccaattct tcttgtcaca aaactttaac gtggctgaac aatttactgg tcttccaggt
    69001 tcatatgtcc ctgtagaaga aacagtgaaa ggattccgcg aaatcttgga aggcaaatac
    69061 gatgatcttc ctgaagaagc tttccgaagt gttggccgaa tcgaagacgt cgtagaaaaa
    69121 gcgaaaacac taggctacta gaaaggggtg ccccatggat caatatttaa cagtaaatgt
    69181 ggtaactcct gccggtttgg tctatgatca ccatgcagct atcgtcgttg cacgtacgac
    69241 agctggtgag cttgggattt tgcctaaaca tgcaccaatc atcgttccat tgacgattga
    69301 tgaagttcgt gtaaaacgaa cagattcaga tacccacgtc gattggatcg ctgttaatgg
    69361 cggaatcatg gaagttcgtg ataatgttgt ttcaatcgtt gctgatagtg cggaacgtga
    69421 acgtgatatc gatgtatctc gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga
    69481 agaagcaaaa gcaaaacaag atacgaacga attgaaacgg gcaactgttg cgttgcaccg
    69541 agcaatcaat cgtatcaaag tatctaagca ctcataaaat gagatgcgga tgtttatccg
    69601 tgtttaagaa cagtaaaaag aggttatgac agaagtggtc agcttcaaga cataagaagg
    69661 aatttacgaa aattgcttct caaatttttg tgaaatctca gcttattttt cgaagaagct
    69721 acttctggaa caccgtatat cggaggttgt gaaatcagcg ttttgtcctg agtattagag
    69781 aaacaaa
//
LOCUS       DAFBYI010000003        56554 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFBYI010000003 DAFBYI010000000
VERSION     DAFBYI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.003"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            49..164
                     /gene="rrf"
                     /locus_tag="LLD04_000134"
     rRNA            49..164
                     /gene="rrf"
                     /locus_tag="LLD04_000134"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            495..1340
                     /locus_tag="LLD04_000135"
     CDS             495..1340
                     /locus_tag="LLD04_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5647100.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1621..2841
                     /locus_tag="LLD04_000136"
     CDS             1621..2841
                     /locus_tag="LLD04_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK5647101.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2838..5558
                     /locus_tag="LLD04_000137"
     CDS             2838..5558
                     /locus_tag="LLD04_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5647102.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5542..6486
                     /locus_tag="LLD04_000138"
     CDS             5542..6486
                     /locus_tag="LLD04_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5647103.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6555..7565)
                     /locus_tag="LLD04_000139"
     CDS             complement(6555..7565)
                     /locus_tag="LLD04_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5647104.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7745..8089)
                     /locus_tag="LLD04_000140"
     CDS             complement(7745..8089)
                     /locus_tag="LLD04_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5647105.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(8082..8516)
                     /locus_tag="LLD04_000141"
     CDS             complement(8082..8516)
                     /locus_tag="LLD04_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK5647106.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8654..9391
                     /locus_tag="LLD04_000142"
     CDS             8654..9391
                     /locus_tag="LLD04_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647107.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9384..10595
                     /locus_tag="LLD04_000143"
     CDS             9384..10595
                     /locus_tag="LLD04_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647108.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10799..11581
                     /locus_tag="LLD04_000144"
     CDS             10799..11581
                     /locus_tag="LLD04_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK5647109.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11651..12313
                     /gene="trmB"
                     /locus_tag="LLD04_000145"
     CDS             11651..12313
                     /gene="trmB"
                     /locus_tag="LLD04_000145"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK5647110.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12411..14165)
                     /gene="ade"
                     /locus_tag="LLD04_000146"
     CDS             complement(12411..14165)
                     /gene="ade"
                     /locus_tag="LLD04_000146"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK5647111.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14297..15226
                     /gene="tadA"
                     /locus_tag="LLD04_000147"
     CDS             14297..15226
                     /gene="tadA"
                     /locus_tag="LLD04_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK5647112.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15183..16232
                     /locus_tag="LLD04_000148"
     CDS             15183..16232
                     /locus_tag="LLD04_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK5647113.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16229..16507
                     /locus_tag="LLD04_000149"
     CDS             16229..16507
                     /locus_tag="LLD04_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK5647114.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16482..16952
                     /locus_tag="LLD04_000150"
     CDS             16482..16952
                     /locus_tag="LLD04_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK5647115.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16969..17271
                     /locus_tag="LLD04_000151"
     CDS             16969..17271
                     /locus_tag="LLD04_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647116.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17288..17659
                     /locus_tag="LLD04_000152"
     CDS             17288..17659
                     /locus_tag="LLD04_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK5647117.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17631..17990
                     /locus_tag="LLD04_000153"
     CDS             17631..17990
                     /locus_tag="LLD04_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647118.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            18162..19169
                     /locus_tag="LLD04_000154"
     CDS             18162..19169
                     /locus_tag="LLD04_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5647119.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19194..20378
                     /locus_tag="LLD04_000155"
     CDS             19194..20378
                     /locus_tag="LLD04_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK5647120.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20815..22398)
                     /locus_tag="LLD04_000156"
     CDS             complement(20815..22398)
                     /locus_tag="LLD04_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5647121.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEVTVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22976..23119)
                     /locus_tag="LLD04_000157"
     CDS             complement(22976..23119)
                     /locus_tag="LLD04_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK5647122.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23342..23821)
                     /locus_tag="LLD04_000158"
     CDS             complement(23342..23821)
                     /locus_tag="LLD04_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5647123.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23894..24178)
                     /locus_tag="LLD04_000159"
     CDS             complement(23894..24178)
                     /locus_tag="LLD04_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647124.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24256..24450)
                     /gene="ssrS"
                     /locus_tag="LLD04_000160"
     ncRNA           complement(24256..24450)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LLD04_000160"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24522..25808)
                     /locus_tag="LLD04_000161"
     CDS             complement(24522..25808)
                     /locus_tag="LLD04_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK5647125.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25946..26617
                     /locus_tag="LLD04_000162"
     CDS             25946..26617
                     /locus_tag="LLD04_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK5647126.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26583..27071
                     /locus_tag="LLD04_000163"
     CDS             26583..27071
                     /locus_tag="LLD04_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK5647127.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            27084..28031
                     /gene="prmA"
                     /locus_tag="LLD04_000164"
     CDS             27084..28031
                     /gene="prmA"
                     /locus_tag="LLD04_000164"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK5647128.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            28032..28796
                     /locus_tag="LLD04_000165"
     CDS             28032..28796
                     /locus_tag="LLD04_000165"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK5647129.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            29029..31242
                     /locus_tag="LLD04_000166"
     CDS             29029..31242
                     /locus_tag="LLD04_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK5647130.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31253..31699
                     /locus_tag="LLD04_000167"
     CDS             31253..31699
                     /locus_tag="LLD04_000167"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK5647131.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31870..33129
                     /locus_tag="LLD04_000168"
     CDS             31870..33129
                     /locus_tag="LLD04_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK5647132.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33218..34165)
                     /locus_tag="LLD04_000169"
     CDS             complement(33218..34165)
                     /locus_tag="LLD04_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5647133.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34559..35851
                     /locus_tag="LLD04_000170"
     CDS             34559..35851
                     /locus_tag="LLD04_000170"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK5647134.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35866..37635
                     /gene="aspS"
                     /locus_tag="LLD04_000171"
     CDS             35866..37635
                     /gene="aspS"
                     /locus_tag="LLD04_000171"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK5647135.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37744..38619
                     /locus_tag="LLD04_000172"
     CDS             37744..38619
                     /locus_tag="LLD04_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5647136.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38885..40768
                     /locus_tag="LLD04_000173"
     CDS             38885..40768
                     /locus_tag="LLD04_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK5647137.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40843..41127)
                     /locus_tag="LLD04_000174"
     CDS             complement(40843..41127)
                     /locus_tag="LLD04_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5647138.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(41117..41851)
                     /locus_tag="LLD04_000175"
     CDS             complement(41117..41851)
                     /locus_tag="LLD04_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK5647139.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41980..42603
                     /locus_tag="LLD04_000176"
     CDS             41980..42603
                     /locus_tag="LLD04_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK5647140.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42795..43916
                     /locus_tag="LLD04_000177"
     CDS             42795..43916
                     /locus_tag="LLD04_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK5647141.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43923..47051
                     /locus_tag="LLD04_000178"
     CDS             43923..47051
                     /locus_tag="LLD04_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK5647142.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            47070..50264
                     /locus_tag="LLD04_000179"
     CDS             47070..50264
                     /locus_tag="LLD04_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK5647143.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50410..51117)
                     /locus_tag="LLD04_000180"
     CDS             complement(50410..51117)
                     /locus_tag="LLD04_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5647144.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51882..52280
                     /gene="spxA"
                     /locus_tag="LLD04_000181"
     CDS             51882..52280
                     /gene="spxA"
                     /locus_tag="LLD04_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5647145.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52612..53265
                     /locus_tag="LLD04_000182"
     CDS             52612..53265
                     /locus_tag="LLD04_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK5647146.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53491..54489
                     /locus_tag="LLD04_000183"
     CDS             53491..54489
                     /locus_tag="LLD04_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK5647147.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54665..56473
                     /gene="pepF"
                     /locus_tag="LLD04_000184"
     CDS             54665..56473
                     /gene="pepF"
                     /locus_tag="LLD04_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK5647148.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18605 a         9764 c        11729 g        16456 t
ORIGIN      
        1 tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg tggtggcgat
       61 agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct tagcgccgat
      121 tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc aaaaagatga
      181 actagaaata gttcatcttt ttttgtttaa atcttttgct aagttgaact aatagagtct
      241 tttatgaata tcgaagttag tttttcacct aacttcgttt tttatagttg gttattataa
      301 aatagtaata tagctgattt ggattcaatt catatttatt tataaatcaa aaatacagag
      361 aatagtatat aaatgtcctt tcctattctt aaaatgatgt ttgtacttac ttaaattagg
      421 tataattagt atatgttttg tgagaaattt agacttacaa taagcagtga atttataatc
      481 aacgtatagg agacatggat gaaatgaaac taattggtat agatttagac ggtactcttt
      541 tgaattctga ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag
      601 actgttttcc attcatttgt tctggaagag aagtagaaga cattaataat attctcaaaa
      661 aatctggttt aaggatacca gcagtagggt taaatggttc tgttggattt gatggagaaa
      721 aaaagatatt tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat
      781 cgaaatttcc aaccaaaata tatacaaatc tcggaagtta tgaatctcag aattataaaa
      841 ataaaatgaa aaaaattttt cacgaggtag ggagtgaatt ttctatcaaa gagctaaact
      901 atgagcttga gtatgaaaaa tctatttgtt caatccctta taataatatt gaggaaatta
      961 tagaaaagga tgctatcagc atttacaaat tttttatttt tattcctaat cgtcaattga
     1021 aagctaatat atttaaccat ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac
     1081 ctgtaaatat tgaaatcgta cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg
     1141 aatcaatcta caatttaaag aaacctttga gaatcgcgat cggagatagt ttaaatgact
     1201 tatctatgtt tgaaagtgca gatattagtt ttgcaatggg aaatagtcat caagaaatca
     1261 tgaaaaaagc tgattatgtc actgtttcaa atgataaaga cggagtatct caagcatttg
     1321 aaagaattct taccatataa actaattaag gtagtataga acatagaatt ctaaatacgg
     1381 aaaaatggag cataaattcc agtacaaata gtacttcttc agtgtcaaac gaagcgaata
     1441 taacatagat atttaggatt gtgacaaaaa tcatgtcaca atcttttttt cgaataaact
     1501 tattttttga agtcggccat ttatcgtaat ttctttgcat aatacgaaca aattttcggt
     1561 tattgatgaa aaaaaacatt gaaaatggta tgataaaaga aaaaaaggag gaaattttta
     1621 atggtgaaat ttatccatgc ggcagattta catatggatc ggtcatttga aggactgaca
     1681 actcttgata aaactgtaca ggaaaaacta ttgaagacga atctgaccgt tttatcaaat
     1741 atcattgaac aggcaataat aaataacgtt gattttgttt tacttgcagg agataatttt
     1801 catcaaaatc gtccttcatt aaaaatacag aaacattttt cagaacagat gaaacggtta
     1861 gaaaagaacc atatacctgt atttataata ttcggaaatc atgatttcta tcaaaaagag
     1921 cggtattggt tctcctttcc taaaaatgtc catttattta catccgaaac ggtagagaca
     1981 aaaaaaataa cgataaaaag tggagaaact gtctctctca gtggatttag ttatcgtcaa
     2041 ccgtggattc aaaaagacaa agtgatggaa tttccttcta gagaactcac agattaccat
     2101 attgggttgt accacgggga acctggcgtc tctcaaaaag gaaactatgc tcctttccaa
     2161 ccctcaaaga tgcaagaaaa gggatacgat tattgggctt tagggcatat ccatgtgcca
     2221 acggtcttaa atgaaaagca aacaattgtt tatccaggtg cgcctcaagg tcatactaaa
     2281 aaggaacaag cttctacatc tattctacta gttgaattaa gtaaaggaag ttgccaaata
     2341 catccgatta aagtagcaga ggtctattgg aagacaaagg aaatatcatt gaggaaagca
     2401 agaacgacaa aagaggtcat tgttcacatt cgtcagcagc tttcaaaagt agaggatgga
     2461 tttagtttaa ttgagataaa attaaaagat tatgaccatc tcaatacaga tgttttggaa
     2521 cggatacaat caggagagct attagactat ttgttagagg aattctcaga taggataaat
     2581 gatttcttta tttggcgtat ttctctcata gagaatactt tttatacgaa gaccccactt
     2641 gctgcttctg ctaaattgat ggaacagtta tttcaatact atcagacacc tcaagatttc
     2701 caacaaattt taaatgaagt atatagtcat caagaagcgg ctagaatatt gagcgaactg
     2761 ccggaatatc aagaagaaac acttgaaaaa gccaagcagc tgcttaatca agatttttta
     2821 tttgaggagg atcaggaatg aagattttaa ggatcgagat tgctgctttt ggaaaatggc
     2881 gtcaaaaaag ttttgacttt tattcaggca atcagctgat ctatggggga aacgaagcag
     2941 gaaaatcaac gatttatcaa tttattcaag caatattatt tggtttccct tcaaaaggcc
     3001 gtaaaaaaaa ggattataca ccaaaagacg gctcagccta tggcggcaaa atatggctta
     3061 aacatcctgt atatggtgaa tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat
     3121 cgaaggtgtg gctgggggat caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac
     3181 ctttgacgaa agagttattc cagcaggtat ttactttcca gcaagagcag ttgatggaac
     3241 tagatcattt gagagaaaaa gagctacatg atgcgctgat ttctttaggc atcacgggca
     3301 gttctcaagt attccaaaag cggcaagaat attatcagaa agcacaacaa ttattcaaga
     3361 aaaaaggcca aaaattacta atcaatcaaa aattaaatga atggaaagag ctgcaagaaa
     3421 aaattcatca aaaacaagaa caggaagctt attttggcaa gttgatccaa caagaacagg
     3481 aatacgagtc gcaacagcag cagttattgg cacaattaaa agaggcgaac aaacaatttt
     3541 cattagccag acagcaagaa cttaattttt ctttatatga agaatggcaa tctctcaaaa
     3601 aaaacagcac tacaggtctt cctgatgagc aacttctttt agatctggag tctttctcca
     3661 aacggtatca gcaaacaaat gagcgtttgg aagaattaga agcaacactg gaaaagcata
     3721 gcgggatgga tcagcagtca gccagatatt atttttattt agagcaggag caggaactaa
     3781 aagatctcca gcaacttcaa tttacagcgg aacaattaaa cgaagaatgg caaagattac
     3841 aaattgagac tagagaaaca aattcagaat tgaagatttt ggaacaaagg tggcattggc
     3901 agaaaaatcc gttaccggaa gaatttttat atgaagaaga atggaagcat cttctggctg
     3961 aagaaagaag gattgctgat ctagaaagcc agatttctgt gcgtctccaa atagctaaag
     4021 aacagcaaga gatgattgaa gcagaagtga atgacttgga agagacctat ccagcattat
     4081 tacaaaatca gtcagagata gcaccagtct cttcatctaa accgtatatg ctgatggctg
     4141 gcggagcaat aatcattgca ggtatatttg taccaatgcc cttgaaagtg atccttttac
     4201 taattggtgc aggcatgatt ggctatttct tctacaaaag gcaaccaaaa attaaaacta
     4261 aaaatgattt ctctgagata aaaggaatgt ggcaagaaaa actaggacaa cttgatctag
     4321 ccttagctca attagctgaa gcagaagaaa aacaaaagag aactggagaa gaaaaacaga
     4381 ttcttcagca agaagtatat cgttttgcgg atcatcggca cttaggaaag atggatacgc
     4441 tcaaactcat gaaagaatat ggacaggaag tcatagatta tcagaaatta tatggcgtat
     4501 ttgagcggaa acataaaaga gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa
     4561 aatttgagtt tgttaaagaa tggttaccga tacaagataa gacaatttca gaaaaaatgg
     4621 tattgctttc ccgttttata caagagatgg aacagatgaa atttgccaga agctatcagc
     4681 aaaatacttt attgaagcaa gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac
     4741 aagaatacag cgaacaattg actcaagcag gcttatctta tccttcggaa gtacctgtct
     4801 ttttacaaaa agccaaacaa gccgaacagc agatgaaaag atacaatgaa ttatcggaaa
     4861 tgcttactcc actatttcct gaagagctga caaaagagaa attatctgta agactgcgcc
     4921 aactgaaaga aaagcaatcc caactccagc aagaagaaaa agaagcaagt gaaaatcggc
     4981 aacgactcca acttcaagta gaagagttgc aaagagacgg cacattggac gaactttatc
     5041 aacaagctag ccggcaaaaa gcaaaattac aggaattact ggaggaatgg ggagcacttg
     5101 aaatcgccgg gacattctta ggtgatcttt caacagagat gagtgaaaga caattacccc
     5161 agttgttaag agcagcaggt gattatttag gtattttaac aaaaggaaat tatcagaaga
     5221 tattactgaa aaatgataca ctgactttaa cagacggata tacagaattt cccatctatg
     5281 agttatctac aggtacgaaa gatcagctga ttatggcgat gagatttgct tacctctcat
     5341 tagaagctaa ccgttcgata tgcccgatca tcattgatga tggctggctt cattatgatc
     5401 atcagcgaaa gtatcaatta gctgaacttt tagctaaatt tggagaaaag tatcaaataa
     5461 tttgtttttc ttctgataaa gaaatggtaa gctattacca tgagatgaac caaatcgtaa
     5521 aacaattaga aggagcaaat aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt
     5581 gaacaatttg tcctgatcaa aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt
     5641 attgctttta ctttccaaga tacttcaggt actatcgatg gtaaatactg ggatgcttcg
     5701 gaggaggaaa tcaataaatt tacagccggc acagtcgttt ttctaaacgg taaaagagag
     5761 gtttatcaag gaaaccctca agtaaagatt cttcatatgc gcttaacaag ggtagatgag
     5821 ccaggagagc ctagtttata catggaaaga gcccctttga agaaagaaga aatggaagaa
     5881 gaaatcaatc aattgttgtt tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat
     5941 ctacttaata aatatcaaaa acaatttttt gagtttccgg cagcaaaaag aaaccatcat
     6001 gcatttgcca gtggattagc ttatcatact ttgacaatgc ttcacttagg gaaatcaatc
     6061 gccgatgaat atcctgagct gaatcgagca ttgttgtatg ctggtatcat tttgcatgat
     6121 ttaggaaagg tgattgaact ttccggtcct atgtcgacag aatatacgct tgcaggaaat
     6181 ttgatcggtc atctggtatt gattgacgaa gaaataacta aagcctgtct tgcgttgaaa
     6241 atcagcgaag ctgacgaaga tgttctcgta ttacgtcata tggttttagc acaccatggc
     6301 ttattagaat atggctcacc tgtacgtcca agaataatgg aagcagaaat attacatcaa
     6361 atcgacaata tcgatgcttc gatgcagatg atgttgactt cgattcgtca aacagaacca
     6421 ggaaaataca ccgatcggat tttcggtatg gacaaccgaa gtttctatgt accaaaagat
     6481 ttatagcaaa aatttatttt tttcattcaa aacaaaaaaa gcgagacata agtcttcgct
     6541 ttttttgttt tcttttattt tgatgaatca gttgtttctt cagttgaaga ttcttttgtt
     6601 gaatcagttg attttgtgtc agaactcttt gttgaagctg tagtttcact tgagtctttc
     6661 gtacttgatg aagttgttgt gaatgcactc aacacatttt caaaggcatc atctttgatt
     6721 tttacatttg catcttttaa ttcttcaccg attactttag ttgtgaatgc attgtcactt
     6781 agtttagttt cagtagcgat atcttttagt tgatctttgt atttatccat gtcattgcct
     6841 ttattttggt ttttcaccat tttgacaacg tagtattctg tcgcataaga agtaggattt
     6901 gttgtagtaa taacatcgga gatctcaccg tctttcaatt tgaaagctgc ttcttttact
     6961 tctgcaggaa tagttgttgt cgttgagtcg aatttaactt taccgccatc ttcttttgtc
     7021 tctgtatcag ttgatttgtc tttggctaat ttagagaaat catctccatc atccgctgat
     7081 tttttcacat cttttgcttc atcttcactt gataatttga tgatttgtgc ttctacttct
     7141 ggatggaaag atttccaagc agttttcaaa tcgtcatctg taatatctac gtgagctttc
     7201 aagccagctt caaaagctaa attgttacga atatattctt tataagtatc tttcgtatat
     7261 ccagcagctt ctaattgtga ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat
     7321 tctgcatcca cttgcttatc ggttacttta tcgccatatt tgttattgaa tactttataa
     7381 atgatcatcc gttgtactaa ggattggttc gatgattcaa gttttgcttc atcatagaaa
     7441 tcagatacag tgatcgttcc gcctttcatc gttgcgatat ctttattagt atcgcctgaa
     7501 caagctgcta gagttaaaac agccaacgca ctagtagctg ccaaaataat tgattttttt
     7561 ttcattaaaa tttgcactcc tgtaattagt tattattttt atatattaac tataccatat
     7621 cccatttcct ttaaaaacgt tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca
     7681 ttcctaggaa atacgcaaac ttttctcact tatatagtta aatctgttga gaaaataatc
     7741 attattatat ttgttcagga tactctggca gatcagcttt tagtttttct acttgttcat
     7801 tgatttgtgt gatacgtggt tcggcttgga atttaaaatc ttcgatatct tttttgattg
     7861 cttcttggaa agcaggaatc acagattcag ctgtttcttt tgtttcgatc aatgcgcttt
     7921 taaaatgatt caggctgtta tttaaatcat ttgtcagagt tgttgcttct tccaattctt
     7981 cgatcagttt ctttcttgtt tcattcccgc tcctgggcgc caataacaga cctccgacag
     8041 tgccagcagc agctccaaat aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc
     8101 tgctttacga ttgtttcttg aatagctttt agttcttccg atccataact atcttgatga
     8161 ttgacaaagt gaatagaaaa atcatcctct ttgctgtagc gagggactaa atggatatgt
     8221 gaatgaaaaa cagattgata ggctagttca cggttgttat tcaagatatt aagacctttc
     8281 atttctggaa aagctttttc caatgcttga gcaatcttag gcacacgcga aaaaacctct
     8341 cctgcaagtt cgggatcata ttcaaaaata tctgctacat gacgtttagg cacaactaat
     8401 gtgtgtcctt tcgttgcttg agagatgtct aaaaaagcat agaccttgtc gtcttcatat
     8461 attttatagc ttgggatttc ttgattgatg attttgcaaa aaatacagtt ttccatataa
     8521 aacatccttt ctataaagaa taaatacttc agcttaactt taccataagt taaagctatt
     8581 gtaaggaaaa aaacaaaata ccgtcatttt cttcgtggta taataaataa aaataaatgg
     8641 cggaggaaaa agaatgagtt taattatcga tcatgtcaca ggggggtacg gacatctccc
     8701 tgtgttaaaa gatatcagtt tcgatgtaca gcctggagag atagtagggt tgattggact
     8761 gaacggagca gggaaaagta cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa
     8821 aggaacgata gaaatcaatc atctatcctt aaagaaaaat acggaagaat atcgcaaaca
     8881 aattgggtat attccagaga ctccttcttt atatgaagaa ttgacattga gagaacatat
     8941 cgaagtcact gcgatggcct acgatatacc tatggatata gcaatgaaaa gagcagaaaa
     9001 gttgttggag acttttcggt tatctaatcg tcttgactgg ttcccagcta atttttcaaa
     9061 gggaatgaaa cagaaagtga tgatcttatg cgcctttttg atcgagcctt ctttatatat
     9121 catcgacgaa ccatttttag gcttggatcc actagctatc aatgcattgc ttgaattgat
     9181 ggtagaaatg aaaaaagaag gagccggtat tttgatgtct acgcatattt tagctactgc
     9241 agaaaaatat tgtgatcgtt ttgtggtctt acataacgga gagatacgag cagacggttc
     9301 tcttgagatg ttgagaaaag aatttcagct gccggattct tctttggacg aaatctatat
     9361 tgctttaaca aaggagcagg cacatgagta gtttttttca aaaacgttta gcaagacatc
     9421 aaaagaaaat gatgaaatat atgcgttatg tattgaacga tcattttgtt ttagtttgtc
     9481 ttttcttgat tggcggtgtg ggattatatt attctaattg gttgaaaacc ctgcagccac
     9541 cgtttgcaat tggagggctc ataatctctg tattttggat gctctgttta ttcgtgggta
     9601 agatggctac ttttgcagag gcagcagata ttgttttttt acttcctaaa gaaaaagaaa
     9661 tgcaaaccta tttggaacga gcttttcgct attcttgcat ttttccgttg atctttttga
     9721 tattggcatg cggattggcc atgccattag tggtggttag tacggagcaa tccttttcga
     9781 tgtttttcgt ctatctgatc atattatgga gtttgaaatt cagtcatctg caaatcaaga
     9841 gagcagaatt gtttcgacta gatcaacaag ttattcgtta ttggaaatta ggctggtttt
     9901 tttcttctct gatctttttg tttctatcat tttattggct aatatgggca ggaatagcag
     9961 gtgcattagt acaggcaagt ctatattact ggtttttgtg gaagcaacaa gaaaaaggat
    10021 tggattggga gaaaatggtg catgtagaag aacataggtt gcatcagatc tatcaattta
    10081 tcaatttatt cacagatgtt cctgaaatta cggcaaagat caaaagaaga cgctatttgg
    10141 atcctatatt gactcatatt aaaagagaat caaaaaacac ttatctttac ttgtttgcaa
    10201 gaagattggc aagaggttct gattttagtg gtctttattt ccgattagta attataggtg
    10261 ggttattgat tgccggagta gtcgactttt attttagtct gattatcggt ttcttatttt
    10321 tatatttgat cggtttccaa ctgttacctt tagcgaacca gttccgctat atggttctcg
    10381 ttcagcttta tccaatccat aagaatcaga agaagcaggc gattcagtat ctactcacat
    10441 gggtattaat tatcgcgtcg gttatttttg gtttgattgc tgcagtcgta ttagatgggc
    10501 agcagcgatg ggttccgcta gcggcttatt tgattgttac tatgctcttt gttaaactat
    10561 atgtaccaac tcgtttgaag aagatgtcag actgaaaaca aagctgtgta gggtaaaaat
    10621 gagaatgaaa aatagagcgt tgacagtaag aatgctgttc gttctatttt ttttgagaaa
    10681 agctggtttt ttgaatggca attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg
    10741 ttcaggcgac tagaataata agtactgcaa aacaaagagt aagaggacgg gagaatgcat
    10801 gatggagttt cagttagacc aagagtggcg gttacgtccc atcaaaggcg acacaggaaa
    10861 aacatacata ggcatgcgtg atgaggacaa agtctttatt aaacgaaaca cgactccgat
    10921 gttggctgct ctttcaaggg aaggaattac accaaaactt gtttggacaa aacgaacggg
    10981 gaatggcgat actttgacag cacaagagtg gctggaaggt cgcttgctga ctcctaatga
    11041 gattggtcaa agaaacgacg tgattgatgt tttgtaccat ttacatcatt caaattctct
    11101 taaaaccatg ctgacccgta tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa
    11161 atatgaaaaa gaattaccgg aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg
    11221 ttttcttcgt gagcagatcc cgtcaataga taaggaaaac ttatcagtag tacacggaga
    11281 tgtaaaccat cgtaattggt tagtatgtaa aaattattta tatctagtcg attgggattc
    11341 aatcatgttt gcagaccctg cggtggatat tgcaacgatt ttaggaaatt atgtgccact
    11401 gtctagctgg aatcagtggc tgatcagtta tggcatacgc ccgactgacg aaatgcttga
    11461 aaaatttcat tggtatgcgg ccatgaattt gctccaagaa atcaccagat attgcaaacg
    11521 tgaagatcat cgacggatga acgaagaaat tttacaatta aaacgaattt tcagtggctg
    11581 aggtttggcc caaactctgc ggagatttgg ccagaccttt ttaattagag gaaaggaatg
    11641 gagataaata atgcgtgtcc gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc
    11701 gaattttgta attagtgatc caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa
    11761 cgatcatcca atccacattg agattggtat ggggaaaggc cagttcatca ctggaatggc
    11821 aaaagcccat cctgaaatca attatattgg agtagaaatg caggtcagtg ttgtttcgat
    11881 tgcactagac aagttgatcg aacaaccgct tcctaacctg aaacttctac atgtggatgg
    11941 atcagcgttg acagagtatt tcgctgacag cgaagtagat caaatctatc tgaatttttc
    12001 cgatccatgg ccaaaaaaac gtcatgaaaa acggcgattg acatataaga cttttcttgc
    12061 tgtagatgaa cagattcttc gtccaaatgg agaaatccat ttcaaaacag ataatcaagg
    12121 attgtttgag tattctctgg caagtttttc tcaatatggc atgatcctca aacaggtatg
    12181 gttagattta catcaaagtc aatttgaagg caatattatg acggagtatg aagaaaaatt
    12241 ttcttcaaaa ggtcaacgta tttatcgagt agaagctcaa tttcaagata agaccaaaaa
    12301 gaaaagtctg tgaaactaac tttctgcttt cataaaaaca ttaatatcca taaataaatc
    12361 ccagaaaatg aacattacag atgtaatgta ttttctggga tttatttgat ttaaaatgta
    12421 tttatttcat cggtttttcc acaaggtgga atcaattgga agcgagaaaa tgaaaataat
    12481 cctttgtcgg tgatcttcaa ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa
    12541 aaaggatcaa aggttttagc tgctcctagt tgatgtgctt tagttattaa ttcattcaac
    12601 tgttcataga cgatcgaaaa aggttcctgt gtcatgattc cgcctatagg aagagaaaca
    12661 caagctattt cctgctgttt gttgacaacg gtcatcccgc cacctttttg aatcaaaaga
    12721 ttagcagcat acagcatatc ctcgtcgttt gtcccaacga caactagatt atgcgagtca
    12781 tgggcaatcg ttgtcgctat cgctccttgt ttcagctgaa atcctttcac tatgcctaat
    12841 ccaatgtttc ccgtttgatg gtgacgctcg atcaccgcaa tctttaattg gtctttttcg
    12901 attgaaggaa tgaatcgttc gccttgtcgg tccacttttt ctaccaaatc tttagtcact
    12961 agactattcg ggataatttc gatcacatgg gcttgtgaag aagaaagaag cagctctaac
    13021 gaatgtggat ttaatggttg agcgtttagt tttggcagac aacacgtata tgaatttgaa
    13081 tgatcagcaa ataaatggtc ttggagttgc ccagattgaa caactaatgt tcccttatag
    13141 aaaacttcat gaattggcag cttggtaaga tcgtctgtta agaagaaatc agcttgatac
    13201 ccaggggcaa tcgcacctag tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc
    13261 atctgaatgg cttgtaatgg atcgattcca agtgcaatag cttgacggac gatatgatcg
    13321 attgatcctt cttttactaa atcattgatc agtttatcat cggtgacaaa gaagcatctt
    13381 cttgaatttt caggtgtgat cgccggtagt agcgatgcca gatcttttgc aacggtccct
    13441 tctcgaacca ttaaatacat accaagatcc aaacgttctt tagcttcttt tgcagttgta
    13501 gcttcatgat ccgtccgaat acctgctgca ggataaacat ttaactcttc catgccgata
    13561 ccagctgcat gcccgtcaat ttttttctta tgctggtgca tcaattggat cttttctagg
    13621 atgtctgttt cattatttgc tactgcttga tagttcatga cttcagctaa gcctaaaact
    13681 tcgggttttt gtaaaaaagg ggctaatttt tttgcattta aatttgcacc attggtctca
    13741 aaaggggtta caggtacaca agaaggtagc ataaaaaaga tattcatgag tgtttgtctt
    13801 gcatcttcta tcataaactc aagacctgta ctcccagcaa tgtttcctat ttcatgggga
    13861 tctgtcacta cagtagtaac tccatgaagc aaggatactt ttgaaaattc gcttggtgat
    13921 aaaagtgagc tttcgatatg cacatggcca tcgatgaatc ctggcactag atattgccca
    13981 ttagcattat gtacgctttt tccttcgtat tcaccgattc ctacgatcat tccttcgcag
    14041 attgctacat ctttttccat gatttctttc gtaaagacgt tgacgatctt agcatgcttg
    14101 atcactaggt cagcaggctt ttttttactt gctacttcga tatatttcct taatgttgtt
    14161 tccaatggag tgcctcccaa gataaatttt tataagtata acataaattc ggtttttgcc
    14221 aatatttttt tgtaaattca ttttatttga aacaaaaaga tcaaaaagac gtatgttttt
    14281 tgtggagggg agaataatgg aaataaaacg attatcggat caaatgataa gtgaaagttt
    14341 tgccaaacat gcccaagacg tatatatatt gccagcttca gaagtatttt acatctattt
    14401 tcgctatgga aaaaaacgaa cattctggaa gaaagtctct tcggcagatg gacagcaact
    14461 gatctcaaga tttaagtatt tgggacagat ggatatcgga gaaaaaagaa aagctcagtt
    14521 aggtgcattt acttattctt tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg
    14581 agattacctt caacgagaga gtttagtgat tcggctatta tacggtactt ttgaaacacc
    14641 ccatcgttgc tttagttctc gagattttgc aactatttta cagaaaaccg tcagacgtgg
    14701 attatacctt ttcagcggac cagtcggatc aggcaagact tctctaatgt atcggttggc
    14761 tttggaagaa aaattgcaag tgattactat cgaagatcct gtagagatag aagaaccgtt
    14821 ttttctgcag ctacagatca atccgaaaat agcacagact tatgatacac ttttgagact
    14881 cgcattacgg catcgtccag acttgctaat cattggcgag ataagagatg aatcaactgc
    14941 acatgctgcg gtaagagcag cactaacagg gcaccgggtt tttgcaaccg tacatgcacg
    15001 caatcttgaa ggtacattgg tcagaatcca tgaactgaca aagaaaaaag aagagctatc
    15061 tgaatgttta gctggtgtca tttatcaaga acttttggaa gatgccaatc accatccttc
    15121 tgttttatgg agttatcaat ttatggagga agaatcagta aaaaaatcat gggaggaaag
    15181 ttatgaagaa gctcaggaaa atatttggca gtcagaacta ttctaaagga ttaacaagaa
    15241 gccagcaaaa ccagtttgca caaaccatgg gagaactgct tttatctggt tttagtctgc
    15301 aagaatccct ttatttatta ctcacgatcc gttcgtttcc acctgttatt ttggcaaata
    15361 tccaacatca gctaaaggaa gggcaagcgt ttagtgaggt actttcgcaa ataggatatg
    15421 gagcagagca actggtacag atcgagttag ctgaacgtca tgggaatctg ccgacaactt
    15481 tgttggaaat tgccaaacag tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc
    15541 tgcttgcata tccgatgcta ttgctcacct ttttgctagg tatcttgttt agtatgcggt
    15601 tgttcgtttt gcctcagctt attggttcag gaatggtcat gcaagagcat tggggtattc
    15661 agctgataca agtctttcat tggtatgttc tactattcat cctagcggtt actctattgg
    15721 ctttactagt aaggtatcgt ctgatgaagt tgtctcctgt tgatcagagt gaatggataa
    15781 gcagacaatt attgattgga ccgatatatt ccttgtatca atcggcttat ttgtcactag
    15841 agattggaaa gctttttcat gaaggtttcg aattaaggca aatcatagtc tgcttaaagg
    15901 aaacgaaaca agaaagtctg attcagcatc ttgcctctaa gttgattcaa ggattggagg
    15961 caggtatgtc attggctgag cagttccaga attatcgttt tctatcagaa gaattcagta
    16021 aaattgtttt acagggagag gcaaagggaa gtttaggtaa agaactgatt ttttacagcg
    16081 aatggacaag aaaacagctt ttcaaacgtc ttcatcactg gatgcatttt attcagccaa
    16141 tcattttttt gggcgtagcg gctttgattt tattagttta tgccgctgtt ttattacctg
    16201 tttacggaag catagaggag gttttgccat gaaaaagaga aaaatatctt attatagcgg
    16261 ttttacattg atcgaaatgc taatcgtctt actgatcatc agtgtattag tgttgttgtt
    16321 cgtccctaat ttgtcgcgtt ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat
    16381 ccagttggta gacacacaaa aagagctcta tgcgcttcaa aataatggaa gagttcctac
    16441 tgtggaggaa ttgttgaatg aaggctatat caaacgagaa catgcagaaa tctatcagcg
    16501 gccttgaagg taaggtttta gcatcaggat atacgttgtt tgaatcactc ttactgattt
    16561 tattacttac aacgatcctt tcttttccag ttctcgcatt ttctgcatgg aagaatgaat
    16621 tggcagtcta tcaatttttc ggccaatttg aaaaacgaat ctattctacg caaaaaatcg
    16681 ctattgtgaa tcagatccaa acaggctttt attggaatag tgaagagaat caaatcattt
    16741 ttcatgttcc gaatccagat aaaataaatt gggaagtatt agatattcct gatgagatta
    16801 ccttgaaacg ccatgcttct attacttttg ctgctagaac cgggaatgaa agttcactaa
    16861 aagcttatca attttattgg gaagaaaaag agcggacgat tacgtatcaa ttccagatag
    16921 ggagtggtcg ctatacaaaa agaattgaat aaaacaggat ttgttttgtt ggagtccctt
    16981 acagcgctta tgatcgcttc tgtctgtgtc tacttagtga gctttggcca gctgtttttg
    17041 cttcaggcag aaaagaaaag tggggaggaa ctgctattta ttcgaatgat ttatgaagat
    17101 atcaagacgc agcggatgta tcacaaaaga ccagagcgaa gggaagaaat gatcccatac
    17161 gatatccaaa ttgtcgataa taatggagta atcgaaaaag tgtttgtcaa tatggagaaa
    17221 gaagcgatca gtattgaaaa aacaagtaat tccagcattt acgctgattg aatgtctaat
    17281 tggactgatg atattgagca gtctattgct cacattcaat cttttgattc agcaaaccat
    17341 ccaagtaagt gaatttttgc agcgtaaaga tcaaaaagag tggctaatat ttttagcaca
    17401 attagaagaa gaactcaaga gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta
    17461 cacgatcgag gataaacagt atatcatcga aagatatcag gcaatgatac gtaaaagaag
    17521 aacttccggc ggacatcaac ctatgttaac aagtatcact gagcttcaat tattggaaaa
    17581 agaacacaca atttcttttt atgtacactt tgaaaatgga gaagatggat atgccatatg
    17641 gacaaaaaac gatcagtaga aaatgttata agggctctat tttattttct gtcttgcttt
    17701 tatttctttt atgtacgttt ttattgatgg cagcattaga aagctaccgt ttgtcaatgg
    17761 atcttaatat acgaatcaaa aattattata tagcaaaaat aatgaagcag atggctttat
    17821 cggaacagtc tacgcttgcc gaaagttcgg aaggagtttt ttactacaca actggatctg
    17881 taacatatca gtgggtacaa cagtccctat tcttagaggt ggaagtctca ccgtttatct
    17941 ttcggttcac agaagaagta aaaaatgata cagatactag tacagaatga tggaatgttc
    18001 gtgaaaacgc cctcttttct gttataacac tgtttttaat aaaaaaatgt ttgccgtttc
    18061 acaaataaaa aacagtgaaa acgattgaaa ttttgtcaat tgttcgatag aattaaacag
    18121 gtgaaaaaat tgttataata tatctagaaa tgaggtgcac catgttacca gaaaaaatgg
    18181 aacaagggtt taatcaaaac ctggaagcta ttcagctttt acagaatgca ttgggaactt
    18241 cctttttaga agcctatgtc gaaaatgcag aaaatttaat agatgattat caagtacgtg
    18301 tggtggatgg agtgccgaca aaagagacaa cgcaacggat cacagctctt tatgaagagt
    18361 tgaagaaact ttctttcgag ccagaagaat ggcgcagact gtctcaatta cttttattga
    18421 agggcagcca gacagaacat ttgcaggcga accatcaact cactcctgat agtatcggtt
    18481 ttttatttgt ttttcttatt gaacaactat acacaaataa aaaagcaccg gttaagattc
    18541 tggatattgc ggcaggaatg ggcaacttgt tattaacagt tcttttaaac ctatcgaatg
    18601 cggggtacca aacagaagga attggtgtgg atattgatga tacattgttg gctgtcgcag
    18661 cttctacaag tgatttgaca caagcaaatg ttcaatactt tcatcaagac gggttgcagg
    18721 aattattaat agatcctgtc gattttgcaa tcagcgattt gccaatcggt tattatccga
    18781 atgacgaaaa agcaaaagag tttttaacaa gtaccgaaga aggccatagt tatgcacatc
    18841 atttattgct agagcaatca atgaaatatg tgaagccgga tggttttggt ttgtttttga
    18901 tgccgagtgg atttttagaa acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag
    18961 aaggttatct tcaaggaatg attcaattgc cagatgaatt gttccgtaat aaacaatcgc
    19021 aaaaaagtat tttgatctta cagaaaaaag gaccacaagc acaacaagta aaagaagtct
    19081 tgcttgtgaa acttgcttca ttaaaagaac cagagaaggt gacggaattt ttcaacgagt
    19141 ttaaaaactg gaaatcatct gctctgtaat cattcaacga gaaggagagt catatgtcta
    19201 aaacaattgc aatcaatgct gggagttcaa gtttgaaatg gcaattatat caaatgccag
    19261 aagaaaccgt agttgcgaaa gggatcgtcg aacgtatcgg tttacaagat tctattttta
    19321 ccatcaagta cggcgataat caaaaattcg aacaaatcat ggatatcgaa aaccacgaag
    19381 tagcagtaaa aatgctgtta gatcagttga ttgaactgca aattttaagc tcttatgatg
    19441 aaatcaccgg agtagggcac cgtgttgttc acggaggaga aacttatggg gattcagtag
    19501 tgatcgatga gaaagtaatg gaccgcattg ctgaattagc agaatttgct cctttgcata
    19561 atcctgcaaa cttaatgggg atcaaagcgt ttaaaaagat tttacctgac attttgagtg
    19621 tggctgtatt cgatacatct ttccactcaa caatgccaaa gcaaaattat ttatatagct
    19681 taccggtgtc ttattatgaa gatttcaaag taagaaaata tggcttccat ggcacaagtc
    19741 atcgttatgt gtcggaacgt gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa
    19801 tcatcacttg ccacttagga aatggtgtat cgatcacagc agtcgatgga ggtaaatcca
    19861 tggatacttc tatgggattc acaccattag ctggggtgac aatgggtaca cgttcaggtg
    19921 atatcgatcc agctgttctc ccatatttaa tggaaaaatt agagattgat attgatgaaa
    19981 tgatcaatgt tttgaacaag aaatctggtc tgctaggcct aacaggattg tctagtgata
    20041 tgcgtgatct tgaaaagaac tatgaaaaag aacatattcg tttagcttat gacattttcg
    20101 tggaccgcat ccgaaaatac atcggcggtt atgtgacaac gatgaatggt gttgatgcaa
    20161 ttgtctttac tgctggtatc ggcgagaatg acggacatgt acgtagtgaa atcatcaaag
    20221 gtatgtcttg gtttggttgt gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag
    20281 tagatatctc tacaaaagaa tcaaaagtaa gagtattagt gatcccaaca gatgaggaac
    20341 taatgattgc ccgtgatgtg gagcgtctgc gcggctaagc caaagcattc gttaggataa
    20401 aagatgaaac gcatgcaaga gggaattctt gcatgtgttt tttttgcgga gaagaccgca
    20461 aaatggtaat ttaacttcaa tttcccttct tttttacagg atcttcttag ggaattttgc
    20521 ttggaaaagt ggtcaaaagg gaaaagactg gcattttatt tattgatttt caggaaaatc
    20581 ccaatatttt attttcattc atctttataa gggacattac atatgtaaaa agaaaaaaca
    20641 tttttagatc agcctcgtgg gctgtcgact ttattcttcg ctttatgttt agctagaaaa
    20701 ccgatccgaa acattcattg atcgaataat aaaaacttga ttttccacct gtactttgcc
    20761 tgaatcgtga aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc
    20821 aatcagttgg ccgagcggct ggcggctgat ctgctcctga tcaataacga gtgcttcttg
    20881 gaagtgttgc tgcaaaaagt agcggtcatg gctgactgct aggagcgttc cttgatagtc
    20941 ttgcagcatt tcttcaattt cttctcgagc atagctgtcg aggtgattgg ttggttcatc
    21001 caaaatcagg acgttgattt tttctaaaaa gagtttcagc aacgccaaac ggattttttc
    21061 accaccggat aaatctttga tccgtttacc cacatcgctt tgataaaagc cacttttggc
    21121 taattgccgt cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg
    21181 gctatcggga tcggcaaact gcagctgctg cggcaaataa ccgatttgca agctactacc
    21241 taatttgatg gtgccaccgt aaggcttttc ctcaccgagt aataatttga gtaaggttga
    21301 ttttcctgca ccattgtcgc cgatgatggc taaatgatct ccacgataga ctgcaaaact
    21361 gctgtctttg aatagtactt gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac
    21421 ttctttgcca gattttccac cattagacaa gctttgcagc cgatttttcg ggggaggtgg
    21481 cggtttgacg agtgtcagtt tcgccaggcg gcgttccagt tctttggctt ttttgaagaa
    21541 ggactcattg tctccttcat gtgcccactg acgaaaacga cgaatcatga ctttcaaccg
    21601 ctggacttct ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt
    21661 caagactcga aaacggctgt agtttccggg atactcaatc aatgcacctt cttccagttc
    21721 ccatgttttt gtggtgactt gatcgagaaa ttgccgatca tgagaaacga ccagaaccga
    21781 tttttttgta ttctgtaaat agttttccaa ccagcgaata ctgttaagat ctaaatgatt
    21841 ggtcggttca tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg ctaaagcgac
    21901 tttaactcgt tcgcctccgc taagttctgt taaccgtgct tcctgacggt gtggaatccc
    21961 taagccttgc aagatcgctg caatccggtc ttctaatacg taaccgccta gttcttcaaa
    22021 gcgatgctgc agctcaccgt aacgggccag acacttctct aagtttgtat ctggttctgt
    22081 catcaatgct tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag cggcagaatg
    22141 gagcaagtag tggttgacgg tcacttcgga aactgtaagt tcttgttcga ccgtcccgat
    22201 ctttaagcct tttttgcgac tgataacacc gctgtcgaca ccttctctgc cggtcaagat
    22261 ttgtaaaaag gttgatttgc cagttccgtt gataccgact aaggcaattt tttcaccagc
    22321 ttcaatttga caggataaat cctcaaataa tggattcccg ccgattgttt ttttaataga
    22381 aattgcttgt atgatcatga tctctctcct tgagaccact caaaaaggcc atggttatcc
    22441 atggcctgtg ttatttaaac tcagattatc atgagaaaaa taccttgaga tggccattga
    22501 ttttacgtga attcaactgt tttggacaaa cttgtcccgt caacagcaac tgtaaaacca
    22561 attgcatggc taaatgcgat atggtatcgt tggtaatttt cattacttgt tattttagtc
    22621 atgcgcatca tagtattttc cctccttcgt gtagttgtct tttccagtgt acctgaaagt
    22681 gtcagaaagt tcaactagcg ctgacattta cttaatattt atcgatgtgt aattctgtca
    22741 gagagctttt tttatctgac tgagggtaat gtctcgtgga ccacatcatt gtattctgag
    22801 ctaaaccagc gttcaggcga aatcatatgg tgtttgataa aaaactttgt ctacatgact
    22861 tccttttgcc atttgcttaa taccgttgca ttaaacagct cttctctttg gaaatctcac
    22921 caacatcgcg ttcaggagta tcgttgtggt tgcaatggca agcggtagcc tttcatcaat
    22981 tcttgaatga tttaccgaat gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa
    23041 attttagttt ggcgataaaa tcggtatccg ccatcgatac ttcatatttt ttcgtatcag
    23101 attcatcgga aacttccaca ttgacgaacc aataagcttt ggcatgctta ggtcgtttta
    23161 aaccacacca gggcgactaa tcgccctggt gttcactttt ttttatttta cttgaactca
    23221 ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt
    23281 tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt
    23341 gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa
    23401 gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt
    23461 ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga
    23521 tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt acattattca
    23581 agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag
    23641 cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga
    23701 catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag
    23761 ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca
    23821 taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga
    23881 aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg
    23941 tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa
    24001 aagctgttaa aatacccatt acgcctaaaa cagcatatgc agctccgtaa aattgtagga
    24061 atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata
    24121 aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga
    24181 acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt
    24241 attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg
    24301 cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt
    24361 taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca
    24421 aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa
    24481 gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc
    24541 tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta
    24601 tcttttggct gataatactg agcactcttc agtttatctg gtaaatattg ctgattgacc
    24661 caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca
    24721 cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata
    24781 tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga
    24841 tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct
    24901 aatacagcgt tcactgtacg agccgcagca gcgggattac ctaaacctat atcttcataa
    24961 ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg
    25021 agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata
    25081 acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct
    25141 tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta
    25201 gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga
    25261 agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt
    25321 aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc
    25381 tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg
    25441 atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct
    25501 aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct
    25561 acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta
    25621 gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga
    25681 atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact
    25741 agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc
    25801 tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca
    25861 agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt
    25921 gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg
    25981 gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac
    26041 accagaagga agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga
    26101 cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa
    26161 accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca
    26221 gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga
    26281 tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa
    26341 attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc
    26401 gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat
    26461 cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa
    26521 tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga
    26581 aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag
    26641 actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct
    26701 tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag
    26761 aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg
    26821 atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag
    26881 tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc
    26941 ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcagc
    27001 aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc
    27061 caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg
    27121 aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg
    27181 aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa
    27241 atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt
    27301 tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc
    27361 tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag
    27421 cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct
    27481 gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg
    27541 catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct
    27601 tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag
    27661 caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt
    27721 cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg
    27781 atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata
    27841 tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg
    27901 tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt
    27961 ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag
    28021 aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg
    28081 ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg
    28141 aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg
    28201 aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt
    28261 cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat
    28321 ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat
    28381 gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag
    28441 cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt
    28501 tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc
    28561 aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct
    28621 tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac
    28681 acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt
    28741 atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt
    28801 cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa
    28861 tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg
    28921 aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat
    28981 ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa
    29041 gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat
    29101 gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga
    29161 aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta
    29221 aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa
    29281 atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc
    29341 gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac
    29401 cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat
    29461 agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct
    29521 caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa
    29581 tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc
    29641 cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat
    29701 attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac
    29761 atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac
    29821 ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca
    29881 atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa
    29941 gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa
    30001 attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg
    30061 gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc
    30121 tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa
    30181 ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt
    30241 actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc
    30301 ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg
    30361 aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac
    30421 tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc
    30481 caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat
    30541 ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt
    30601 tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc
    30661 ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa
    30721 aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta
    30781 cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc
    30841 tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata
    30901 tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt
    30961 ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg
    31021 gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg
    31081 ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc
    31141 catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa
    31201 gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc
    31261 agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat
    31321 tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga
    31381 ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa
    31441 tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc
    31501 ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat
    31561 tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg
    31621 acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat
    31681 tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg
    31741 cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct
    31801 tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg
    31861 ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg
    31921 tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc
    31981 aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta
    32041 ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca
    32101 ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat
    32161 atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag
    32221 aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag
    32281 atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa
    32341 gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct
    32401 ccgattgatc acataaaagg tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc
    32461 ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct
    32521 tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa
    32581 ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat
    32641 gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa
    32701 gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt
    32761 tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta
    32821 gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc
    32881 aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca
    32941 caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat
    33001 agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa
    33061 gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac
    33121 aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc
    33181 ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat
    33241 caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata
    33301 cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc
    33361 aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat
    33421 cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag
    33481 gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg
    33541 aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt
    33601 ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat
    33661 ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt
    33721 ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat
    33781 ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt
    33841 ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat
    33901 gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct
    33961 cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga
    34021 tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa
    34081 aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg
    34141 aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa
    34201 ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact
    34261 ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag
    34321 tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc
    34381 ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta
    34441 agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg
    34501 ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat
    34561 gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca
    34621 gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac
    34681 accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt
    34741 ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga
    34801 aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc
    34861 gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg
    34921 acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac
    34981 tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat
    35041 ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt
    35101 gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca
    35161 tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga
    35221 agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt
    35281 ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact
    35341 agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca
    35401 agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc
    35461 aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga
    35521 ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga
    35581 aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga
    35641 ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc
    35701 caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc
    35761 aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga
    35821 agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg
    35881 atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg
    35941 gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga
    36001 atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa
    36061 tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca
    36121 gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg
    36181 aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata
    36241 cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta
    36301 agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc
    36361 gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca
    36421 cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg
    36481 ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat
    36541 ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca
    36601 gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc
    36661 ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac
    36721 ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc
    36781 aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca
    36841 ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa
    36901 tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg
    36961 aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat
    37021 gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca
    37081 ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc
    37141 aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat
    37201 gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa
    37261 gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaactaggc
    37321 ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc
    37381 tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga
    37441 ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga
    37501 gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg
    37561 agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg
    37621 aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac
    37681 aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt
    37741 tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta
    37801 tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat
    37861 atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg
    37921 ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta
    37981 ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg
    38041 acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt
    38101 gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta
    38161 tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt
    38221 ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc
    38281 tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc
    38341 gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta
    38401 gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt
    38461 gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct
    38521 ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac
    38581 gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca
    38641 tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat
    38701 cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt
    38761 atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt
    38821 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
    38881 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
    38941 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
    39001 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
    39061 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
    39121 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
    39181 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
    39241 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
    39301 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
    39361 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
    39421 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
    39481 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
    39541 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
    39601 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
    39661 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
    39721 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
    39781 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
    39841 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
    39901 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
    39961 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
    40021 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
    40081 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
    40141 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
    40201 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
    40261 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
    40321 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
    40381 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgatcggact
    40441 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
    40501 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
    40561 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
    40621 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
    40681 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
    40741 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
    40801 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
    40861 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
    40921 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
    40981 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
    41041 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
    41101 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
    41161 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
    41221 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
    41281 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
    41341 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
    41401 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
    41461 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
    41521 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
    41581 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
    41641 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
    41701 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
    41761 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
    41821 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
    41881 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
    41941 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
    42001 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
    42061 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
    42121 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
    42181 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
    42241 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
    42301 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
    42361 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
    42421 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
    42481 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
    42541 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
    42601 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
    42661 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
    42721 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
    42781 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
    42841 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
    42901 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
    42961 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
    43021 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
    43081 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
    43141 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
    43201 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
    43261 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
    43321 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
    43381 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
    43441 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
    43501 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
    43561 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
    43621 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
    43681 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
    43741 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
    43801 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
    43861 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
    43921 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
    43981 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
    44041 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
    44101 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
    44161 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
    44221 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
    44281 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
    44341 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
    44401 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
    44461 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
    44521 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
    44581 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
    44641 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
    44701 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
    44761 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
    44821 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
    44881 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
    44941 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
    45001 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
    45061 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
    45121 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
    45181 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
    45241 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
    45301 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
    45361 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
    45421 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
    45481 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
    45541 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
    45601 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
    45661 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
    45721 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
    45781 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag
    45841 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
    45901 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
    45961 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
    46021 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
    46081 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
    46141 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
    46201 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
    46261 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
    46321 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
    46381 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
    46441 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
    46501 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
    46561 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
    46621 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
    46681 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
    46741 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
    46801 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
    46861 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
    46921 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
    46981 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
    47041 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
    47101 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
    47161 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
    47221 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
    47281 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
    47341 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
    47401 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
    47461 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
    47521 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
    47581 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
    47641 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
    47701 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
    47761 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
    47821 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa
    47881 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
    47941 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
    48001 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
    48061 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
    48121 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
    48181 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
    48241 tcgatttcgt ctatggcgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
    48301 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
    48361 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
    48421 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
    48481 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
    48541 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
    48601 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
    48661 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
    48721 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
    48781 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    48841 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    48901 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    48961 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    49021 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    49081 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    49141 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    49201 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    49261 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    49321 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    49381 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    49441 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    49501 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    49561 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    49621 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    49681 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    49741 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    49801 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    49861 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    49921 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    49981 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    50041 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    50101 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    50161 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    50221 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    50281 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    50341 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    50401 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    50461 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    50521 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    50581 ggtctcttct ggaagcatat gaaattcttt ctcagctagc tgaaaaatct ctttttctgg
    50641 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    50701 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    50761 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    50821 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    50881 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    50941 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    51001 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    51061 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    51121 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    51181 aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac
    51241 agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt
    51301 ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg
    51361 tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa
    51421 ataacgttat ttgatttatt tgtttatagt aaattttatt tagtaatcat tgctttaaaa
    51481 tactacgctt atataacaga gaaaataagc gttataataa agaacagtat tatttaaaat
    51541 gacagaaaac attactatta aagaaataaa aaacataaaa aatcttaaga aatcggtttc
    51601 atttagcttt tgagataaaa taatagtaca gttttatacg ctgtttcata tgtttagaaa
    51661 atttgaatta gatttgaaaa ctctaatttt taaatgataa aaaaagcgag aatgctggtt
    51721 ttactgtata ttaaccttag ataactttca tttttttaaa aaaattatga aaaaaaggta
    51781 gctatttaaa agttgattat gtataataga aacagatggg agattattat gatcttctgt
    51841 tacaaaaaag tgattcttat cacgaaaagg agtggtgcac gttgttaaca ttatatactt
    51901 ccccaagttg tacttcctgc agaaaggctc gtgcgtggtt gcaagaacat cagattccat
    51961 ttgttgaacg taatattttt tcagaaccat tgaacagttc tgaattaaaa gcgattttac
    52021 aaatgacaga agacggaaca gaagaaatca tctcgacacg ttccaaagta ttccaaaaac
    52081 tgaacatgga tttagatgat ctgccgttac aagaattatt ggaacttgtg caaaacaatc
    52141 ctggattgct tcgccgtcca atcatgattg atgataaacg tctgcaagtt ggttttaatg
    52201 aagatgaaat tcgtcggttc ctaccgcgcg atgtacgaca attagaatta cgtcaagcac
    52261 aattgatggc tggattatag aaggataata cgaaacttgg aaaagactat tacaaagata
    52321 gtttttccaa gtttttttgt ttttacaaaa agtattcttg attagatgta taaaaaggta
    52381 gtagaagatg atgattgcac gaaaaataga gagggaaaat tagctgaaaa gtcggctata
    52441 tagtattcgt ctgccaaaaa tgttatagtt aaataagata gcttgaaagg ttgaaaaatc
    52501 cgctggtttt attaaattcg cttaacagcg agattttttt tctttacatt taagctgatt
    52561 tcgctacaat gaatctatat taaacattgt ggcaaagtga ggtgtagcaa aatggaaatg
    52621 gaacatatta atgaaaatac gatccgtgta ctaattggtc atgaagattt agaggaacgg
    52681 ggcgtttcat ttttggattt acttggcaat cacaaagaaa tcgaaaattt cttttatagt
    52741 attttggaag aagttgatat cgaagaggag ttccgaggaa gcgaagctgt cactttccaa
    52801 gttttgccaa aaggagacgg attagaactg tttatcagta aaaatcttcc ggatgaagaa
    52861 atttctcaat cagaagattc tactgttgat tcagaagatg tgagtgactt tcttcgtcag
    52921 cagattattg gcagcgatcc ggaggaagaa ttatctggaa gcgccctaga agaacaggaa
    52981 agacaagcaa cttttgcttt tgaagatttt gaacaagtga tccaacttgc aacagatgtc
    53041 gaactagagt ctgcttggac tgatttgtat ttattcgaag agcgctatta cctagtcgtt
    53101 cacttctgga tggaaaatct caaccaagcg gatgtagaga atcagattgc tcgaattttg
    53161 gagtttgctg aaaagagcga tcgtacacca gaaacattga atgaatatgg acaatgtctg
    53221 atggcgcgta atgctatcga gcgaacgaaa ttttattttg tataaatcag cattatagta
    53281 agagactgtg acaaaaattg tgtcgcagtc tttttttcgt ataaatgtgt tcaaaagctt
    53341 gcatctgcgg taacaagccc aaccaaacga aaaatattgg gaactatttt tgtttagctg
    53401 ttcttattac ttgatgcaga atagcttttt cacgccctct tttttcgtgt aaataaattc
    53461 ttagacgtaa ataagttcag agggagaaaa atgctgcttg caatgaatga atataataag
    53521 tgctgttttg ctaaacatgc agaaaaagga aagaaatatt attgtcgtgg ctgtcgaaaa
    53581 agagtgattt tgaaaaaggg aaagaaaaaa tgtgctcatt ttgcccatca aaaatcagat
    53641 aactgttcgg tctttagtga aagagaatca aaagagcact tacagttaaa ggaatgcttc
    53701 atggattggc tgggacagtc agtagaacca gtatttttgg aagcctattt gccgaggttg
    53761 cggcaaaggc cggatatttt gttggcgaat ctagcaatag aaatccaatg ttctagactg
    53821 tctcaccaga gatttattga aagaacccaa aattatttga ataacagtta ccaagtatgg
    53881 tggatacttg gtaattcttt tcttggacaa tcgcaattct cattgataga aaaaagttgc
    53941 tgctactata atagaaaaag aggagtgcat tgctggaaag ctgatctcaa gaaacaaaag
    54001 ctttatttgt atcatcatat tacagagact gtatcagggc atatttcctt ttgcagtagc
    54061 tgctggactt tttctagtag ggaccttaaa gaaatattca ctagtaacga aattaaaatg
    54121 aaccaaatga aaaaaataga aaaagtgagt gaagatggga agaaatggct gacacgccaa
    54181 cttattcaca agcaaaagaa tacagtatcc atacaagaac aatgctactt gcgtcataaa
    54241 catcttttgc atctatcatc gtggatctat caaaaaagtc ggtttttctt ttatttacaa
    54301 gaacaggttt ttctttatcg aatgttgtac gaagaagcct tggaaaacca gaaagttcct
    54361 gatttcaact catggctttg ccaaataaaa gaacataaaa gggaatggct ttttcctatg
    54421 attgatgaag agatggtcta tcagcagttt tttaacgaat gcatacactt gtcttctttg
    54481 aaaaaatagt aagtataaaa ataggctagg aatcggtttt ctatgtaaaa caagatttag
    54541 ttatgctaaa attgatggaa gcagacacat gaatgagtag aaaagttaga tgctgtgaca
    54601 tgtggatgtt agactatgat ctattttgat agatagagct aagggaagaa aaggagtgga
    54661 tttcatggaa gtaaagcagt tgccaaaacg agaagaattg cctgaaaatt taacttggga
    54721 cttgaccaag atcttttcaa gcgaccaaga gtttgatgag aaatatttgg aattatcaga
    54781 agagttaaaa caatctgaaa aacacaaagg aacacttgat caaggcgctt ctcaattttt
    54841 aaatgcgatt gaattcgtat tgagggttta tcgccaaact gaagtcattt atgtatatgc
    54901 gcaccttaaa aacgatcaag acactggaaa tacagattac caagcgcttt atgcaagagc
    54961 aagcagtctg ttttcgaaag ttagtgaagc cgtttcctgg tttgaaccag aaatattgca
    55021 attgtcagat gaccagattt ggcaatattt caaagaagaa ccaaaattgg aagtctatcg
    55081 ccattatatc cagcaaatag tagataatcg agcccatgtc ttatctgctg agcaggaatc
    55141 tcttcttgct ggagcaggtg aaatctttga tgcttcaagt gatacatttg ctgttttgaa
    55201 taatgcagat ctagtttttc caacgattga aggagaaaat ggtgaaatag tccaattatc
    55261 tcatggcgtg tatggtcagt tgctagaaag cacggatcga agggtgcgcg aagcagcatt
    55321 taaggggttg tacagtgttt acgaacaatt tagaaataca tttgcttcta ctttaggcac
    55381 acatataaaa ggacataatt ttaaagcgaa agtccgtaat tacagctctg ccagagaagc
    55441 gtctttgagc aataatcata ttcctgaaag tgtatacgat actttggtag acgtggtaaa
    55501 caagcatttg cctttgttac atcgatacat ggaattacgg aaacgtttat tagaagtgga
    55561 aaaactgcac atgtatgatc tttatacacc ggtcttaggg gaagctccaa ttacctttac
    55621 gtacgaagaa gcaaaagaaa aagctttaga agcactgaaa ccaatgggtg aagaatacat
    55681 ggccatcgta gaaaaagcat tctctgaacg ttggatcgat gttgtcgaaa ataaagggaa
    55741 acgaagcggt gcttattctt cgggaagcta tgacacaaat ccatatattt tattgaattg
    55801 gcatgatacg ctggatcagc tatttacgct tgtccacgaa atgggacata gtgttcatag
    55861 ttatttcact cgttcgaacc agccttatgt gtacggcgac tactccatct ttttagcaga
    55921 aattgcttcg acaacgaatg aaaatatcct aacggagtat ttattggaaa cagaaaaaga
    55981 ccctcgtgta cgggcttatg tactcaacca ttacttggat gggtttaaag gaacagtttt
    56041 ccgccagaca caattcgctg aattcgagca tttcatgcat acggaagatg aaaaaggtgt
    56101 gccattgact agtgaatacc taagtgatag ttatggtaaa ttgaatgcaa aatattatgg
    56161 tccagcagtc gaagaagacc cagaaattaa attcgaatgg tcacggatac cgcattttta
    56221 ttataattac tatgttttcc aatactcgac tggcttttct gctgcttcag cactggcgaa
    56281 gaaaatacta aaccaggaac cagaggcact agagaactac ttggcttacc taaaagcagg
    56341 taacagcgat tatcctgtgg aagtaatgaa aaaagcagga gttgatatga cacaggctgc
    56401 atatattgaa gatgcaatgt cgatgtttga acaacgtcta aatgaattag aagaattgat
    56461 cgatcgttta taaggctttc tatttttgat tttctcgaat agaaacgaaa aaaatacgtc
    56521 ggctctcttg taagggagtc gacgtatttt ttta
//
LOCUS       DAFBYI010000004        56296 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFBYI010000004 DAFBYI010000000
VERSION     DAFBYI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.004"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..189
                     /locus_tag="LLD04_000185"
     CDS             <1..189
                     /locus_tag="LLD04_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5647149.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="LLD04_000186"
     CDS             complement(1055..1585)
                     /locus_tag="LLD04_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5647150.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="LLD04_000187"
     CDS             complement(1582..2199)
                     /locus_tag="LLD04_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5647151.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="LLD04_000188"
     CDS             complement(2781..3002)
                     /locus_tag="LLD04_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5647152.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="LLD04_000189"
     CDS             complement(3180..4712)
                     /locus_tag="LLD04_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5647153.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="LLD04_000190"
     CDS             complement(4848..5933)
                     /locus_tag="LLD04_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5647154.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="LLD04_000191"
     CDS             complement(5948..6667)
                     /locus_tag="LLD04_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5647155.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="LLD04_000192"
     CDS             complement(6664..7974)
                     /locus_tag="LLD04_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5647156.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="LLD04_000193"
     CDS             complement(8001..8591)
                     /locus_tag="LLD04_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBK5647157.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="LLD04_000194"
     CDS             complement(8909..9970)
                     /locus_tag="LLD04_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5647158.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="LLD04_000195"
     CDS             complement(10538..11605)
                     /locus_tag="LLD04_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5647159.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="LLD04_000196"
     CDS             complement(11625..12104)
                     /locus_tag="LLD04_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBK5647160.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="LLD04_000197"
     CDS             complement(12121..12582)
                     /locus_tag="LLD04_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5647161.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLRTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="LLD04_000198"
     CDS             complement(12596..13846)
                     /locus_tag="LLD04_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK5647162.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="LLD04_000199"
     CDS             complement(14224..15675)
                     /locus_tag="LLD04_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5647163.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="LLD04_000200"
     CDS             complement(15996..16760)
                     /locus_tag="LLD04_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK5647164.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="LLD04_000201"
     CDS             complement(16788..17486)
                     /locus_tag="LLD04_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK5647165.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="LLD04_000202"
     CDS             complement(17498..18277)
                     /locus_tag="LLD04_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK5647166.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="LLD04_000203"
     CDS             complement(18293..19237)
                     /locus_tag="LLD04_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5647167.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="LLD04_000204"
     CDS             complement(19283..20062)
                     /locus_tag="LLD04_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647168.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="LLD04_000205"
     CDS             complement(20442..22517)
                     /locus_tag="LLD04_000205"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK5647169.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="LLD04_000206"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="LLD04_000206"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK5647170.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="LLD04_000207"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="LLD04_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK5647171.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /gene="era"
                     /locus_tag="LLD04_000208"
     CDS             complement(24783..25682)
                     /gene="era"
                     /locus_tag="LLD04_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK5647172.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26092)
                     /locus_tag="LLD04_000209"
     CDS             complement(25697..26092)
                     /locus_tag="LLD04_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK5647173.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="LLD04_000210"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="LLD04_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK5647174.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="LLD04_000211"
     CDS             complement(26581..28773)
                     /locus_tag="LLD04_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK5647175.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="LLD04_000212"
     CDS             complement(28797..29768)
                     /locus_tag="LLD04_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK5647176.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="LLD04_000213"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="LLD04_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5647177.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32008..32454)
                     /locus_tag="LLD04_000214"
     CDS             complement(32008..32454)
                     /locus_tag="LLD04_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK5647178.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32481..32657)
                     /locus_tag="LLD04_000215"
     CDS             complement(32481..32657)
                     /locus_tag="LLD04_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK5647179.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32819..33247)
                     /locus_tag="LLD04_000216"
     CDS             complement(32819..33247)
                     /locus_tag="LLD04_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5647180.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33339..34199
                     /locus_tag="LLD04_000217"
     CDS             33339..34199
                     /locus_tag="LLD04_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK5647181.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34293..34643)
                     /locus_tag="LLD04_000218"
     CDS             complement(34293..34643)
                     /locus_tag="LLD04_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5647182.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(34659..36386)
                     /locus_tag="LLD04_000219"
     CDS             complement(34659..36386)
                     /locus_tag="LLD04_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK5647183.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="LLD04_000220"
     CDS             complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="LLD04_000220"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK5647184.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37670..39106)
                     /locus_tag="LLD04_000221"
     CDS             complement(37670..39106)
                     /locus_tag="LLD04_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5647185.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39103..40506)
                     /locus_tag="LLD04_000222"
     CDS             complement(39103..40506)
                     /locus_tag="LLD04_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5647186.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40736..41638
                     /locus_tag="LLD04_000223"
     CDS             40736..41638
                     /locus_tag="LLD04_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5647187.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41679..43565)
                     /locus_tag="LLD04_000224"
     CDS             complement(41679..43565)
                     /locus_tag="LLD04_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5647188.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43662..44514)
                     /locus_tag="LLD04_000225"
                     /pseudo
     CDS             complement(43662..44514)
                     /locus_tag="LLD04_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44849..46633)
                     /locus_tag="LLD04_000226"
     CDS             complement(44849..46633)
                     /locus_tag="LLD04_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647189.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46633..48384)
                     /locus_tag="LLD04_000227"
     CDS             complement(46633..48384)
                     /locus_tag="LLD04_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647190.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48526..48750)
                     /locus_tag="LLD04_000228"
     CDS             complement(48526..48750)
                     /locus_tag="LLD04_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK5647191.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49001..50587
                     /locus_tag="LLD04_000229"
     CDS             49001..50587
                     /locus_tag="LLD04_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK5647192.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50653..52428)
                     /locus_tag="LLD04_000230"
     CDS             complement(50653..52428)
                     /locus_tag="LLD04_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK5647193.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52639..53541)
                     /locus_tag="LLD04_000231"
     CDS             complement(52639..53541)
                     /locus_tag="LLD04_000231"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK5647194.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53820..56165
                     /locus_tag="LLD04_000232"
     CDS             53820..56165
                     /locus_tag="LLD04_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5647195.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16684 a        11714 c         9118 g        18780 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacgcaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    32041 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    32101 aaattctttc attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat
    32161 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    32221 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    32281 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    32341 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    32401 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    32461 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    32521 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    32581 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    32641 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    32701 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    32761 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    32821 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    32881 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    32941 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    33001 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    33061 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    33121 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    33181 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcaatc aatgtctgtt
    33241 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    33301 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    33361 tcattacttt ttttgtcatc tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag
    33421 ccactatggc ccaatatcct accgttgagt tcaatttatt ccgcagaaca tttgtgacag
    33481 atgagaaaac attgatgcag gcactgcaag atgcactaaa agaaaaagcc cttgtcctac
    33541 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    33601 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    33661 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    33721 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    33781 cagatgtcgt tttgctaggc gtatccagaa cttccaaaac gcctttgagc ttgtttttag
    33841 ctaacaaaaa tttaaaggtt gccaatctac ctttgatccc acaagcacat attcccaaac
    33901 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    33961 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    34021 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    34081 gcgtagtaat caatgtcgct tctttatcta tcgaagaaac agcttcactt attttgaatg
    34141 ctttagacct agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac
    34201 agtttttctt gtctttttct taaaaaaact ggactgtgtc agcgaagaag attcgttgat
    34261 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagcgtc
    34321 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    34381 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    34441 tgttccattt ctactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac
    34501 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    34561 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    34621 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catttttttg
    34681 ataatcgctt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    34741 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    34801 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    34861 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    34921 aattctgtcg gtgcttcttc acggaatttt tccatcaaag cagagatttt tgcagagcct
    34981 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa
    35041 atcgtttgca agccgtcata caatgttttg ccttgttttt tatagtaagc tgctacttct
    35101 gctaataaga gcaatgcttg aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    35161 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    35221 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    35281 tccgcaattg ctgtaggcaa ttcgctagaa acgattgatt tcaataccgc tgcattttca
    35341 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt
    35401 tggtttcctg tcaatacttc ataggagcca tttggcaaac gaacggctgc gcctaaacgg
    35461 tctgcatcag gatccgttgc aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc
    35521 gcatactcga aagcagagtg ttcttctgga ttcggtgatt tgactgtagt gaaatccggg
    35581 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    35641 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagctt caaatcttta
    35701 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    35761 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc gtcgtcagat
    35821 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    35881 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    35941 ggattgtggg atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    36001 gaaagctctg gtgttggacg taaactttcg aatacataag aagggatatt gtgatgtgct
    36061 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    36121 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct
    36181 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
    36241 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    36301 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    36361 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    36421 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    36481 agaaaagatt cttttatcaa gcacggttac aagtattcgc ataaatcgga ttcagtagat
    36541 ttcacgcaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    36601 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcaatcccac
    36661 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    36721 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg
    36781 tcaacccagc ttctctaaat ggtgcagtta atggatccaa tcctacatag atgaatactc
    36841 cgtctgctgc caaatcaaac tcattgccag ttttgacatt acgtgctttc acacctgtca
    36901 ctaccatttc atttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    36961 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    37021 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    37081 ctcctccgat cactactaga cgtttgttac ggaaaaatgc accatcacaa actgcacagt
    37141 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    37201 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    37261 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    37321 ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag
    37381 gatagttttc gatttctgcc gtattgttca tttggccgcc aggagcgcct tgttcgatca
    37441 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggccctg
    37501 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    37561 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    37621 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcgttcg
    37681 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    37741 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    37801 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    37861 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    37921 agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    37981 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    38041 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    38101 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    38161 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    38221 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    38281 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgactttc
    38341 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    38401 tacagcctac tttgttctcc ggatctactt catgagcgat cttcgttgcg atggcacttg
    38461 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    38521 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    38581 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    38641 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    38701 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    38761 cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    38821 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    38881 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    38941 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    39001 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    39061 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    39121 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    39181 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    39241 atccatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    39301 caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt
    39361 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    39421 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    39481 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    39541 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    39601 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    39661 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    39721 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    39781 tttgaaaaac gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    39841 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    39901 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    39961 ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc
    40021 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattaa tcagccaagc
    40081 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    40141 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    40201 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    40261 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    40321 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    40381 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    40441 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    40501 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    40561 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt cttctggatt
    40621 tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca
    40681 aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt
    40741 attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt
    40801 tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg
    40861 ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt
    40921 cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt
    40981 agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc
    41041 ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg accaactgca
    41101 aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa
    41161 tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat
    41221 tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacaagtg atatgtgtgg
    41281 gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa
    41341 acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat
    41401 gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt
    41461 tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga
    41521 aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc
    41581 tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa
    41641 agaatagtta agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt
    41701 gcatcgtctg gcccgaccgt ttccccttga ttttccaaaa tatccaaata atccatgtaa
    41761 ttgatgacaa tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg
    41821 aagcgaagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa
    41881 tatttccctt ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct
    41941 tcagaaacca tcccgattgc atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa
    42001 ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt
    42061 gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt
    42121 acaatttctt tacctgatct tttctgtcta tcatctgttt catctgcttc ctccttccaa
    42181 aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta
    42241 atcacatctg ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt
    42301 ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca
    42361 ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc
    42421 tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga
    42481 tttctaagct taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca
    42541 acaggtgtca taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg
    42601 attgcccaat ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca
    42661 taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg
    42721 aatgtgaata ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag
    42781 aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt
    42841 tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca
    42901 atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat
    42961 atggtcgtta gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc
    43021 attaatgctg cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct
    43081 gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt atcaaacata
    43141 acaaatgtgc cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca
    43201 cgaatcatcc cgtcagcagc taggatagca aggaatggtt ggaaacagcc cgatattcca
    43261 tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg
    43321 tttcctgctt ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga
    43381 tactgccctc ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca
    43441 tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca
    43501 ttgatatttt ccttcccgcc gacattttct acgatttttc tcgctaattc ttggtatttt
    43561 cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc
    43621 ttatttcata cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca
    43681 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag
    43741 ttgaacgtct cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    43801 tttttttgaa taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca
    43861 tctaggacgc gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa
    43921 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt
    43981 atagtgaaca ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc
    44041 aatgcgataa atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc
    44101 ttcactgttt tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag
    44161 caatacattt ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc
    44221 cgtcaatgca atatacacag aatcattcaa ctggacttgt agagcctctt ttgtatattc
    44281 aatgatttca caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa
    44341 ctgttgtgaa agcccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat
    44401 cgggtcaccc actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt
    44461 tctttcatcc ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgttttg
    44521 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
    44581 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
    44641 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
    44701 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
    44761 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
    44821 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
    44881 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
    44941 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
    45001 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
    45061 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
    45121 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
    45181 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
    45241 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
    45301 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
    45361 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
    45421 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
    45481 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
    45541 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
    45601 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
    45661 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
    45721 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
    45781 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
    45841 ttggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt
    45901 tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac
    45961 gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt tcccgcatat
    46021 ttcggtaaat acgagaactg aatttctggt aataccagat gacgattcct aaaatcggca
    46081 agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca acgaaagaag
    46141 aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg
    46201 tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac
    46261 gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga taggtcttca
    46321 atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg actatgactc
    46381 caaacaagta cacaccagca aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca
    46441 gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacaggg
    46501 caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg aacttcatca
    46561 gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat tccgattgat
    46621 aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct gtttttccca
    46681 cattcgttca taccagccac ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat
    46741 tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta aacggtgagc
    46801 tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc
    46861 tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag gatttttgat
    46921 caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca gataaagaga ctccacgttc
    46981 tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga taaatgctaa
    47041 agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc
    47101 tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca taaccgattg atcctaataa
    47161 agcattcaat gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc
    47221 atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag ttttcccaac
    47281 aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg ctggtgattc
    47341 ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg ccggggtctg
    47401 cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc
    47461 ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca
    47521 aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga tcgtgccttc
    47581 caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt
    47641 aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa taattgcatc
    47701 ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg ttttaatgac
    47761 tttgatccca gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga
    47821 atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg
    47881 cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat
    47941 cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag attccagctc ctgctacatt
    48001 ctggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc gatatttttg
    48061 gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc
    48121 caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa
    48181 gaaagctgca aggatcaata ataagatcca ccaggaaatg atctttattg ataaattctt
    48241 atcggcgatc tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc
    48301 cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgac gaaaaaacca
    48361 acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag agcataggat
    48421 tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta cgaaataaaa
    48481 aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt ttttcccttg
    48541 gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt gccccattga
    48601 catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta aatagtcttt
    48661 catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag caataatagc
    48721 aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt tccgaattgc
    48781 tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc tttagtttca
    48841 tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac cagcaagaca
    48901 caccacgaaa aaaaattagg cataccgaat ctttttcttt aattttttct ttctgaggtg
    48961 tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc aatccaacaa
    49021 acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag ctgaagtaaa
    49081 tggaacagtg gatgtaccaa agggaaaaag tttttttaaa acacttcttg cttactctgg
    49141 accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca cttcgatcac
    49201 tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta gtctaattgc
    49261 tatgctttta cagtatatgg cagcaaaatt aggaatcgtg acccaaatgg atttggccca
    49321 agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct tgactgaatt
    49381 agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc tctacttact
    49441 ttttgatatt ccattggtga ttgccgtctt tattactgta ttagatgttt tcgttctctt
    49501 actactgaca aaagtcgggt ttcgtaagat cgaagcactg gttgtctgct taattttcgt
    49561 cattttattc gtttttgtct atcaagttgg cttgtctaac ccagattggc acagtttatt
    49621 tgctggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg gcggcgaaac
    49681 tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata acttatatct
    49741 tcattcagca gtttcacaaa ctcggaagat cgatcaccaa aacgaagaag atgtagcaca
    49801 agctgtacgt ttttctactt gggattctaa tatccagttg tcaatggctt ttctagtcaa
    49861 tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc
    49921 cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg gtattttgat
    49981 tgctgtagca aaatctggta tcttgtccac tttatttgct gtagcacttc ttgcttctgg
    50041 ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag gattcatcca
    50101 tatgcgtatc ccgatttggc taagaagact ggtcacacga ttgctttccg tgattcctgt
    50161 attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacatg ccgctttgaa
    50221 taacttaatg aatgaatccc aggtgttctt ggcttttgcg ttgccatttt cgatgattcc
    50281 tttactgatg atgaccaaca gccaagcgga gatgggtagc cgatttaaaa attcattcat
    50341 cgtaaaactt cttggctggt tctctgtgat tagtctaact tacttgaact tacgaggatt
    50401 accaggacag atcgaaagtt tcttcggcga gcaagccact tccgctcaac tagcattggc
    50461 tgatcacatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat ggacgattgg
    50521 tgatttatat aaaggaaaca aaaaatatga acagcttttg caggaaacgg tagaagaaat
    50581 cgaataaaaa ctgatttttc cacacaaaga cccttgatag ttaggttaaa aatctagcta
    50641 ccaagggtct ttttatttag aagataaagc ttttttcaga tttaatttac tgaaaactag
    50701 catgcttgtt actacgatga attgcgttaa tggcacgcct gcaccagtgt agacacttaa
    50761 aaaatagtcc tttttttctg gcgcataaga acaaaaccat gagacaccga aaagaaaaac
    50821 caattgtgtc agtattaatc cggtgaccac aatcaaagag gaactataat aggcacgatc
    50881 agcttttgca aatacggttc ctagtatcat aagtaatatc cctgctattg aagcaatcag
    50941 cacgggaata gccagaactt ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt
    51001 tgttaattgg aaattaggtg ttctcggttc attactatcg atccaattct ccattagcgg
    51061 aaggtttaat aattcttgag cctgtttgac ttgttcttgc gttccgaaac tataatttcc
    51121 tccttgatgg aaatctttat atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa
    51181 tacgattggc agatcactca tcgtctttcc atctcctgat cccatcagat agaactcttt
    51241 tttctttttg atggttccct tgtgataggc ttcttctaat tcttggttga cttttttcca
    51301 aaaatcattg gtttcttttg cattgttttg gtaataacca gattcgctta ccacatctcg
    51361 caatgcccaa aagatgatat ctccgggtaa ttcttttact tttccacctt gagtccacgc
    51421 gtgagtcgta tataattcgt caatacgggt agaaacagta tggaatgtag gagatgcggc
    51481 taatgcttta tttagtgcag catgtgagac ccatatctca ctgttttctt tattcaagtt
    51541 cgtttcatca tctattcgga tcagttgttt catcaaacgt ccaaattctc cactggaacg
    51601 gtcattgatg gttgcaatac cgtatgttgt ctcattttta tattcgatcc actgatagac
    51661 gatggaaaag ccaactatag gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga
    51721 gagaagaaag agtcgttttt ttaacttctt tacttctttg aaagaaaagt cttgccctag
    51781 caagatctgg acgaaactga tacccaaagc tactacagta aaaggaagaa gccagataga
    51841 atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa agccctaaaa accatggaag
    51901 caattttctg ctagaaccgt atttgcggta ataaatagct aataaacaag cgataatgat
    51961 ctcaacagcc ggaatcacga gggcattacg atatgttcga gtggaatatt catgtgtaaa
    52021 actaatcgga gaaaacaaaa aataaaaata gatacttaaa agaagatact tgttcttcac
    52081 aatcggttta aacgcgagtg caaccaatat actggcaaaa atactgaaca tgccgaacaa
    52141 aactgcataa gaaagatgca gtttatgtgc aagtactaaa aataagctgt aggagattcc
    52201 tttggaaagt gttgtagtcg tatactcccc tagccattta ccatccaaaa tattttgagc
    52261 atagtgaatc agcagctgat catcataggt cgccgacata tctgttccat agattgcttt
    52321 actatataaa tacaaccgaa taattgtaaa taatacggct atgccaagaa taaagtatgt
    52381 atgtttcttt tttagttggt cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt
    52441 tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt tttacttttc tatcagtata
    52501 ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa
    52561 aagcactcct ttgtttctta aacaaaaaag gacaaggcag actgcccaat ccttttttgt
    52621 tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct aataactcag ggaacacttc
    52681 gggatcgaat gcttgcgctt tgaacatgga aatttctttt ccatatggtg cgtaagcttt
    52741 ctttttatct ttaccgacat atggggtttc taatattttc gaaatgttgt caaacattgg
    52801 atcatggacg attttgttca atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg
    52861 ccgatcttta tgagaacctt gtggattttt agaatcattg atatgcagta ctttaagccg
    52921 atctaatccg atgaccttat caaactcttc cattacacct gaaaaatctt cttttatatt
    52981 atatccggca tcattcgtat gacatgtatc aaaagtaacg gataattttt cattcaattt
    53041 tactccatcg atgatttgcg ctaattcctc aaatgttcga ccgatttccg tacctttccc
    53101 tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc
    53161 ctcaataatt ctagcaatcc cagcttctgc ccctgctcct acatgagcgc ctgggtgaag
    53221 ggtgatttga gtagcaccta aagcttccgc acggacaatc tcttctctca gaaattgtac
    53281 agcaaatgga aacatttctg gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg
    53341 aacgacgata tttgttagtc cgttttctgc cataaatgct tttccggcat caatattcat
    53401 ctcttcgata gcttttcttc tagtattttg aggagcgcct gtatagatca taaaggtcga
    53461 agatccgtaa ctggctgctt cttctgccgc gcctaacagc attttctttc cgctcatgct
    53521 gacatgtgag cctaataaca tggatggatt cctccttgaa aggcaattgt ttcgaacaat
    53581 tcctgtatat tgataagatg atgtacaaag aaaatggttt gtttttcttt gccctgttct
    53641 ccatagtaac tgaaactttc cctaaagaaa agcttaagaa ctctttcata aaaaagatca
    53701 tttcctcacg ttttatctca ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt
    53761 tttttgtcct attatttgct ataattagcg aaataaaacc attagttgag gtgacccttt
    53821 tgccacgcaa aaatactcga aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc
    53881 ctaagattat ttttagagtg tttcaatcat tgactgtttt tattactgtt cttcttttgt
    53941 tgttcgcagc tctaggtatc ggaataggtg ctggctactt tgcctatctt gtcgaagaca
    54001 cacaattgcc gactaaaaaa gaactgcaga cagaactagg aaacattacg gaaacgtcaa
    54061 agattgttta tgcagataat acagagatat caaaaatcca gacagatctg atgcggacga
    54121 ctatttcatc tgataagatt tcgcctttat tgaaaaccgc gattatcagt acagaagatg
    54181 aatacttcga taaacatcaa ggttatgtcc ctaaagctgt tttacgagca ctagtttcag
    54241 aagcgactgg catcggctcg tctggaggat ccactttgac acagcaatta gtcaaacaac
    54301 agatcctaac cgatgaaaca acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac
    54361 aagtagaaaa acacttttca aaaaatgaga tcattactac gtatttgaat gtctcaccat
    54421 ttggtcggaa taataagggg caaaatattg ctggagtcca ggaagctgct agaggaatct
    54481 ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc ttatatcgct ggtttgcctc
    54541 aaagccctat cacgtatagt ccatacacga atacaggcgc gctaaaagaa gatctttctg
    54601 ccggtcttgc cagaaaagat tttgttttat tcagtatgta ccgtgaagga caaattacga
    54661 aagaacaata cgaagaagcg aaagcctatg atcttacaaa agactttctc ccacaacaaa
    54721 tcgcggaaca gactgacaga gaattcttgt attataccgt gatgaacgaa gcaacaagga
    54781 ttattgcaca acaattggca gaaaaagata atgctgatat gtcagattca tcggtcagtg
    54841 aagcttatta tcaaaaagca cagcaaacga tccaaaacaa aggatatacg atccattcga
    54901 cgatcgataa agatatctat gctgcattac aaaccggcgt agaaaattat ggctacatgt
    54961 tagatgatgg tgctggtagc caagtggaga ctggaaatgt attgatggat aacagaaccg
    55021 gacgtatcta tggcttcgtg ggtggtcgga attacagcca gaatcaaaat aaccacgcct
    55081 ttgatacaga acgtcaagct ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa
    55141 tcgatatggg acttgtcgga tcagaatccc gtgtatcaga ttatgcaacg acttggcaag
    55201 aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc
    55261 aaaccgtccg tgagtcactg gaatggtcca acaatatccc agcttatcac ttatatcaag
    55321 atgtgttgaa ttccggtgga tcgaaacaat acgcttacga gcactacttg gcgaagatga
    55381 actacccagc aaatgataat tggggcgttg aatcggctcc tttagggaca gtagatgtaa
    55441 cgacgcttca acagacaaat ggattccagg ccttagcaaa tggtggagtc tacgaagaag
    55501 gctatatcat tgattccatc acagataatg caggtaatgt gatttataaa catgaaagta
    55561 attctgtccg tatttattcc gaagcaacgg catcgatcat gaatgacatg atgcgtagtg
    55621 tgatcaatga aaaaatcact acaccattca aagatgtcat ctcaagctta aacggaaatc
    55681 taggaaatgc agattgggtc ggaaaaactg gttctaccaa tgaataccga gattcatggc
    55741 tgatcgtctc aactccatct atcactatca gtagttgggc aggacacgat gacaatactg
    55801 gaatggacag ccaagcaaga atacgttcgg ctaattatct tgccaatcta atcaatcaag
    55861 cttaccaagc aaaccctgag attttcggca ctgatgagaa atttgactta tcatccgatg
    55921 tgatgaagaa aaaagtatct gggttcacag gacaaacagc tggaaaagtt actgtcaata
    55981 aaaaatccat ccaaacgcca ggaaaaacag tggagtcact ttgggcaaaa aatggtccga
    56041 agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga aaactataca gattactgga
    56101 acaccgcttc agcttatgct agagcgaatc cagagaccaa agaatcagac gataaagatg
    56161 attgatctaa tagattaatc agaattaaag ggagctgcga caaaaatctt gtcacagctc
    56221 cctttttccg aataaatggt gttcaaaagc ttacatctac gatattaaga caaatttatg
    56281 taaaaatgtg aaaaac
//
LOCUS       DAFBYI010000005        50772 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFBYI010000005 DAFBYI010000000
VERSION     DAFBYI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.005"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(87..1238)
                     /locus_tag="LLD04_000233"
     CDS             complement(87..1238)
                     /locus_tag="LLD04_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK5647196.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1392..1922)
                     /locus_tag="LLD04_000234"
     CDS             complement(1392..1922)
                     /locus_tag="LLD04_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5647197.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2021..2371)
                     /locus_tag="LLD04_000235"
     CDS             complement(2021..2371)
                     /locus_tag="LLD04_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK5647198.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2528..4057)
                     /gene="ahpF"
                     /locus_tag="LLD04_000236"
     CDS             complement(2528..4057)
                     /gene="ahpF"
                     /locus_tag="LLD04_000236"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK5647199.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4067..4630)
                     /gene="ahpC"
                     /locus_tag="LLD04_000237"
     CDS             complement(4067..4630)
                     /gene="ahpC"
                     /locus_tag="LLD04_000237"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK5647200.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4774..5436)
                     /locus_tag="LLD04_000238"
     CDS             complement(4774..5436)
                     /locus_tag="LLD04_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5647201.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5620..7266
                     /locus_tag="LLD04_000239"
     CDS             5620..7266
                     /locus_tag="LLD04_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK5647202.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7257..8339
                     /gene="uxuA"
                     /locus_tag="LLD04_000240"
     CDS             7257..8339
                     /gene="uxuA"
                     /locus_tag="LLD04_000240"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5647203.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8447..10000)
                     /locus_tag="LLD04_000241"
                     /pseudo
     CDS             complement(8447..10000)
                     /locus_tag="LLD04_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            10148..11289
                     /locus_tag="LLD04_000242"
                     /pseudo
     CDS             10148..11289
                     /locus_tag="LLD04_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(11415..12494)
                     /locus_tag="LLD04_000243"
     CDS             complement(11415..12494)
                     /locus_tag="LLD04_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5647204.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12597..13037)
                     /locus_tag="LLD04_000244"
     CDS             complement(12597..13037)
                     /locus_tag="LLD04_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5647205.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(13094..13504)
                     /locus_tag="LLD04_000245"
     CDS             complement(13094..13504)
                     /locus_tag="LLD04_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5647206.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13684..14118)
                     /locus_tag="LLD04_000246"
     CDS             complement(13684..14118)
                     /locus_tag="LLD04_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5647207.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(14218..15066)
                     /locus_tag="LLD04_000247"
     CDS             complement(14218..15066)
                     /locus_tag="LLD04_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5647208.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            15455..17221
                     /locus_tag="LLD04_000248"
     CDS             15455..17221
                     /locus_tag="LLD04_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK5647209.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            17323..18582
                     /locus_tag="LLD04_000249"
     CDS             17323..18582
                     /locus_tag="LLD04_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5647210.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18729..20030
                     /locus_tag="LLD04_000250"
     CDS             18729..20030
                     /locus_tag="LLD04_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647211.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            20033..20884
                     /locus_tag="LLD04_000251"
     CDS             20033..20884
                     /locus_tag="LLD04_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647212.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20897..21712
                     /locus_tag="LLD04_000252"
     CDS             20897..21712
                     /locus_tag="LLD04_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK5647213.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21863..23029
                     /locus_tag="LLD04_000253"
     CDS             21863..23029
                     /locus_tag="LLD04_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5647214.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            23259..23906
                     /locus_tag="LLD04_000254"
     CDS             23259..23906
                     /locus_tag="LLD04_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5647215.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            23990..24934
                     /locus_tag="LLD04_000255"
     CDS             23990..24934
                     /locus_tag="LLD04_000255"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK5647216.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            25069..26520
                     /locus_tag="LLD04_000256"
     CDS             25069..26520
                     /locus_tag="LLD04_000256"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK5647217.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            26541..27377
                     /locus_tag="LLD04_000257"
     CDS             26541..27377
                     /locus_tag="LLD04_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5647218.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            27377..28036
                     /locus_tag="LLD04_000258"
     CDS             27377..28036
                     /locus_tag="LLD04_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5647219.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            28162..28851
                     /locus_tag="LLD04_000259"
     CDS             28162..28851
                     /locus_tag="LLD04_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647220.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28857..29813
                     /locus_tag="LLD04_000260"
     CDS             28857..29813
                     /locus_tag="LLD04_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK5647221.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29857..30369
                     /locus_tag="LLD04_000261"
     CDS             29857..30369
                     /locus_tag="LLD04_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647222.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(30625..31173)
                     /locus_tag="LLD04_000262"
     CDS             complement(30625..31173)
                     /locus_tag="LLD04_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5647223.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(31262..32443)
                     /locus_tag="LLD04_000263"
     CDS             complement(31262..32443)
                     /locus_tag="LLD04_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK5647224.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(32679..34187)
                     /locus_tag="LLD04_000264"
     CDS             complement(32679..34187)
                     /locus_tag="LLD04_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK5647225.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            34346..34537
                     /locus_tag="LLD04_000265"
     CDS             34346..34537
                     /locus_tag="LLD04_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647226.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34740..35657
                     /locus_tag="LLD04_000266"
     CDS             34740..35657
                     /locus_tag="LLD04_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK5647227.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            35650..36723
                     /locus_tag="LLD04_000267"
     CDS             35650..36723
                     /locus_tag="LLD04_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647228.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36871..37389
                     /locus_tag="LLD04_000268"
     CDS             36871..37389
                     /locus_tag="LLD04_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647229.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            37370..37762
                     /locus_tag="LLD04_000269"
     CDS             37370..37762
                     /locus_tag="LLD04_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK5647230.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37838..38773
                     /locus_tag="LLD04_000270"
     CDS             37838..38773
                     /locus_tag="LLD04_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK5647231.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38906..39685
                     /locus_tag="LLD04_000271"
     CDS             38906..39685
                     /locus_tag="LLD04_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5647232.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            39678..39995
                     /locus_tag="LLD04_000272"
     CDS             39678..39995
                     /locus_tag="LLD04_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5647233.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            40239..41849
                     /locus_tag="LLD04_000273"
     CDS             40239..41849
                     /locus_tag="LLD04_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5647234.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            42006..42854
                     /locus_tag="LLD04_000274"
     CDS             42006..42854
                     /locus_tag="LLD04_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5647235.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            43085..43564
                     /locus_tag="LLD04_000275"
     CDS             43085..43564
                     /locus_tag="LLD04_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647236.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            43597..45825
                     /locus_tag="LLD04_000276"
     CDS             43597..45825
                     /locus_tag="LLD04_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5647237.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            45954..47597
                     /locus_tag="LLD04_000277"
     CDS             45954..47597
                     /locus_tag="LLD04_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5647238.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(47670..48215)
                     /locus_tag="LLD04_000278"
     CDS             complement(47670..48215)
                     /locus_tag="LLD04_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK5647239.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(48238..48723)
                     /locus_tag="LLD04_000279"
     CDS             complement(48238..48723)
                     /locus_tag="LLD04_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5647240.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(48713..49525)
                     /locus_tag="LLD04_000280"
     CDS             complement(48713..49525)
                     /locus_tag="LLD04_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK5647241.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(49664..>50772)
                     /locus_tag="LLD04_000281"
     CDS             complement(49664..>50772)
                     /locus_tag="LLD04_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5647242.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        16818 a         9250 c         9739 g        14965 t
ORIGIN      
        1 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
       61 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
      121 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
      181 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
      241 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
      301 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
      361 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
      421 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
      481 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
      541 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
      601 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
      661 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
      721 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
      781 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
      841 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
      901 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
      961 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     1021 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     1081 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     1141 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     1201 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     1261 atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa
     1321 aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata
     1381 actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg
     1441 atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat
     1501 ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa
     1561 cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct
     1621 aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga
     1681 gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt
     1741 ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc
     1801 agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata
     1861 atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac
     1921 atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat
     1981 gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt
     2041 ccattttttt gccttttgct agctcatcga tcattttatc taaataacga atttctttca
     2101 tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac
     2161 gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta
     2221 gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt
     2281 cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa
     2341 aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta
     2401 tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa
     2461 aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt tttacaatct
     2521 tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta gctcctgaac
     2581 ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct gcgaaaacgc
     2641 cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga ttcatttcga
     2701 tcgttccatc tagccaatcc gtattaggga gtaagccgac tagaatgaat actcctgaaa
     2761 cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat tctacctgat
     2821 tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc gcaagagaga
     2881 ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct agaacatata
     2941 catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta ccaccaccga
     3001 taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc
     3061 ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc gcacctgtcg
     3121 cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata ttaataaaat
     3181 gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata gggtaactac
     3241 gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca
     3301 tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg acaagtcctg
     3361 tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt ccaccgccaa
     3421 tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa gggccagtga
     3481 ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct ccatttaaaa
     3541 acacagttgg cactgccatg atttttttat catcgatttc tttttggaac atcccacctt
     3601 caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat gcctgtacaa
     3661 cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta
     3721 tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca ctaatttgta
     3781 acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca aaaacgattc
     3841 cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt
     3901 cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct agtttttgac
     3961 tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc agtgctaaat
     4021 attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa atctttccaa
     4081 caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc ggacaaactt
     4141 cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt gcactagcgt
     4201 cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct ggatcaataa
     4261 taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa atacgagaaa
     4321 tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca tctgatgtat
     4381 catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact tcaacaccta
     4441 aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga caaacaaaag
     4501 taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct ttttcactga
     4561 cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt tttttcccaa
     4621 tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg taatttagtt
     4681 ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa aacgaaggtt
     4741 aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact cgggataacg
     4801 ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg
     4861 agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt gttccatgat
     4921 cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt
     4981 attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga aacaagcttc
     5041 atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga ctgctttttc
     5101 ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca
     5161 ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt ctgcagaaac
     5221 aagattaatt ttggaaacat acgtaccact ttgaggtatc gtatacacga gttcttgctg
     5281 acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat gttcctccag
     5341 tgccttttca gatattttcc ttccaggttc taagtcagca tagataattt gtttacgaat
     5401 cgcttgataa gcttgtgatt gcaagctgct tttcatcttt attcctccaa aatcatttct
     5461 atgaacgtag tataacagaa gaggtctgaa aaacaatctg gtatgtcaca ttaactagta
     5521 aatttgtata aatagtaaaa ttttggttga catagcgatt tttattgcat aaaattcact
     5581 tgaatacaaa aactaatatg ttagtttagg aggggaaaaa tgattgattt aaacaactgg
     5641 aggaacttta tccctgaatt atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca
     5701 tcattaagag aaaatggaca aaaaaatcct gtctggatcc atttcggtgg cggaaatctt
     5761 tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga attgtccact
     5821 ggtgtcattg tttgtgaaac attcgatgaa gaaataattg aaagggctta ccaagatttt
     5881 gatgatttga ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa acgtatatta
     5941 gctgcgacag ctgacagtct gtattgtcat ccagattgtt ctgaacactt tgataagatg
     6001 attcgacact ttgaaaatcc tagtttacaa ttagttactt ttgctattac tgaaaaaggt
     6061 tatcgattga aagatacaaa taataaatat ataggaccga ttgcagtcga tataaaaaat
     6121 ggtccaaaga agccatcaca tacaatgagt attcttgctg tgctacttta taaacgtttt
     6181 ttatctggag cttggccgat tgcacttgtc tctacagata atttttcaca aaacggtagt
     6241 caattacgta cttctttgct aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa
     6301 aatacattta tccaatactt atcgaatgaa gaaattgttg cgtttccatg gacgatgatt
     6361 gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa agagggcttt
     6421 tcaaatatgt cgattttcca aaccgcaaaa ggaaataata ttgctccatt tgtcaataca
     6481 gaggacatcc attatttaat catggaagat cattttccca atggacgtcc gaaattcgaa
     6541 aaagctgggg tattcttgac tgatcgtgca acggtagata aagcagatac gatgaaggtg
     6601 acgacttgcc ttaatccttt gcatacgtct ttagctatta tgggttgttt actggggtat
     6661 aggagcatag ctgaggagat gaaagatcca gatttatcgc tgctagtttc tgaaatcggg
     6721 tacaatgaag gcctcccagt tgtggagtct ccaggtatca ttgatccaaa gatctttctg
     6781 gaagaagtaa ttactgtgcg cttttctaat ccaaatattc ctaatatgcc tcagcgaatc
     6841 gccacggata cctctcaaaa ggtggcgatt cgttatggtg agacgattaa aaaatatatg
     6901 gaaatagaag ttaaagaccc gcagacactt gtattcatac cattgactct cgctgcttgg
     6961 cttcgatacc ttattggcat tgatgatcaa ggaaaaccat tcatcccgag tccagaccca
     7021 ctattgacag accttcagca taagctttcc gatattcaac tgggtacgca agatacatca
     7081 catattcatg agaaaatggc tcctattttg tcaaataaag aaatttttgg tgtagatctt
     7141 taccaagcag agcttggcag aaaaatagaa gaaatgttca tcaagatggt agcaggacca
     7201 aatgccgtac gaaaaacatt acaggattac ttacaagaac atggaggaaa tttatcatgg
     7261 aaatgacttt tagatggtac ggatcacagg aagaaaagat taccttagat gaaatcagac
     7321 agattcctgc ggtcaaaggg attgtaggca cattgatgga tataccagta ggagaggtat
     7381 ggcctaggga gaggatcaaa gaactaaaag aagaaattga gtctcgtggg ttaacactaa
     7441 aagtaattga aagcgtcaat gtgcatgatg ccattaaaat tggattacct tcccgtgagc
     7501 agtatatcga aaactataag cagacaatcc gaaacttggc agaatatgga atagaagtca
     7561 tctgctataa ttttatgcct gtatttgact gggtgaaatc tgaccttgac taccgcttag
     7621 aagacggctc atccacgtta gcttttatat ctgatgatat tccagatgat ccaagagaaa
     7681 tcgtcgcgcg catcgaacat tccagcaatg aatttggatt gccgggatgg gaacctgagc
     7741 gcttagctcc tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc
     7801 gagaaaattt tgcctatttt ctccaatcaa tcatcccaac atgtgaagag gtaggggtca
     7861 aaatggctgt acatccagat gatccgccat actcgatttt tggattagct cgaatcgtaa
     7921 agaatcgaga agatttagat tggttgtgtc gcatagtaga cagcccttat aacggaatca
     7981 ctctttgtac agggagtatt gcagaagatc cagaaaatga tgtatatggg attttagcag
     8041 aattttgcca aagagatcga atcccatttg cacatgttcg aaatattaaa tttttagcta
     8101 atcgagattt ctatgaggca ccacataaat ccgaatacgg ttcattagac atgtaccgca
     8161 tactaaaaac aatgtatgac aacggattta acggatacat ccgtcctgat catggaagaa
     8221 tgatctggaa tgagacagga agaccaggat acggcttata tgatcgagca ttaggtgctg
     8281 tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt
     8341 gaaaaaaact gtaattttat tatgtaaata agaaggatgt tcaaattctt gatacataaa
     8401 tccggaggtt gttaataagc attcggattt atgtatctta tttggtctag gaagtaactt
     8461 gattttcttt tttcgattga tccatgagtg cttcttcttt taattcaaag atatattctg
     8521 agatcctcat aatcactttt ttccagtctt ctggtgtgat gattcctttt aaaatataga
     8581 tttttttgat atgagaaggc aattgtttca aagggcagtc gctgataacg atgttgcagt
     8641 tctccataaa atcattttgt attttgatcg attcatttga aaaaatttgt tctatcttaa
     8701 cgatcagatc tttactgata taaaaatcac gcgtatagtt cacaccaatc acaattcgcg
     8761 gctgtttttt gtagcgatca tacaaatgta ctaggtcttc aaccatccat tgcacatttt
     8821 ccttttctaa aataactggt aacaagtgtt gattttgttc tctgaaatcc atataaaatt
     8881 gttcaatggc ggtttggact tctttcaata cgctatgttc ttgataaagc atgaaagtag
     8941 gaaggggcgt atttggttga ttgaagttaa gaaatccatt ataaagttta tttcgtgcga
     9001 atgccaatga gtaaagaatc ttttcttttt caggtataaa caaatcaaat acagtacaaa
     9061 gggactccac catttctctt gaataaagaa catgaggaac gtttgctttt tttaaaagtt
     9121 tgtattgctc caagcttgtt tcttccgatt cgattttcgt gatagaaagt cgagctaaaa
     9181 tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag
     9241 agagatcaat aaaaaagtca atatattcat actctgtgaa ttctttttgt acgtctttca
     9301 cagaagttgc aggaaaattc tctttacata tttttaatag aaaatataat ttatctatca
     9361 taccgctatt tagtcggtca agtacttctt ttttaaaaaa agtattcatc ataaaagcca
     9421 ctacttgcgg atcttcatca taaaattctg tagcagtact tgaaaagatg ttccaataaa
     9481 tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat
     9541 tagaaaagga gatagacaaa ccgtactatg ctaaaaatgc attaatcttt ttcattttcg
     9601 tatacaaata agaggcacta tagtttgttt gctgactgaa actgataatc gaatttgtct
     9661 gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa
     9721 atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt
     9781 tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt
     9841 tgatgtatag taaaaaattt gattcagaca tatggaaatg atctgctaaa tattgtgtag
     9901 taataaagaa acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctttttt
     9961 tcttcaagat aaacgtttta atcgcttctt gagtttgcat gaaagaggct cctttttact
    10021 taaattgtta aaacttgaaa atattactgt ttttttcata aatttagaca aaaaaacagt
    10081 aaacatatag gtagaatctc tactatctta tttcttgcat acaaaaaaat caaggagtga
    10141 gagagttatg agatattcaa ccatcttaat atcaacattt gcagccttaa gcttatcgct
    10201 tactataaat cagacagtcc acacagagga gacagaaaca gccaaatgga cagaaaccaa
    10261 gtgggccaac gtcaatcgaa ttacctttca ggatgaattt tcatccttca attcaggagt
    10321 cgcaaaatta agaaaaggac aaacagcgac catcaaagca aaaattgact attcgggaga
    10381 cttagcacct atactacaat cagctgttac atttgagaat gttgatccca ctttgtcgat
    10441 tggctatgac gagaacactt tgactatcca acacaagcaa gcgatattta ccttcaccgt
    10501 tacattaaat caggacttgt taaaaccagc gctttttact gtcaaggtat cagatggact
    10561 accgaacgat catcatttaa cgccgtatag tcagcggcag acaattgagc aagattcagc
    10621 catcgactca ggaggagatc ttgttgaaga accaacagat aaaccagaaa atgaaaataa
    10681 gccagaagta ccgcctacag aaaatccaga cggagaacag aaaccggaaa tagaacctgg
    10741 agaagaaccc gatacagaaa ctcaaccaga gccagacaat aaatcaaaac ctgaaataac
    10801 ccctggagaa aaaccagatg taactccaga accagatacg gattctggaa accaaacagt
    10861 accggaaaca aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga
    10921 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
    10981 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
    11041 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
    11101 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
    11161 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
    11221 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
    11281 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
    11341 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
    11401 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
    11461 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
    11521 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
    11581 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
    11641 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
    11701 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
    11761 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
    11821 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
    11881 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
    11941 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
    12001 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
    12061 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
    12121 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
    12181 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
    12241 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
    12301 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
    12361 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
    12421 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
    12481 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
    12541 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
    12601 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
    12661 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
    12721 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
    12781 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
    12841 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
    12901 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
    12961 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
    13021 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
    13081 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
    13141 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
    13201 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
    13261 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
    13321 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
    13381 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
    13441 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
    13501 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
    13561 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
    13621 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
    13681 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
    13741 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
    13801 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
    13861 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
    13921 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
    13981 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
    14041 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
    14101 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
    14161 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
    14221 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
    14281 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
    14341 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
    14401 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
    14461 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
    14521 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
    14581 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
    14641 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
    14701 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
    14761 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
    14821 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
    14881 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
    14941 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
    15001 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
    15061 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
    15121 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
    15181 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
    15241 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
    15301 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
    15361 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
    15421 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
    15481 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
    15541 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
    15601 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
    15661 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
    15721 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
    15781 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
    15841 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
    15901 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
    15961 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
    16021 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
    16081 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
    16141 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
    16201 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
    16261 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
    16321 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
    16381 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
    16441 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
    16501 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
    16561 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
    16621 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
    16681 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
    16741 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
    16801 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
    16861 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
    16921 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
    16981 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
    17041 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
    17101 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
    17161 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
    17221 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
    17281 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
    17341 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
    17401 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
    17461 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
    17521 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
    17581 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
    17641 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
    17701 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
    17761 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
    17821 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
    17881 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
    17941 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
    18001 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
    18061 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
    18121 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
    18181 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
    18241 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
    18301 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
    18361 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
    18421 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
    18481 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
    18541 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
    18601 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
    18661 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
    18721 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
    18781 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
    18841 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
    18901 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
    18961 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
    19021 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
    19081 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
    19141 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
    19201 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
    19261 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
    19321 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
    19381 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
    19441 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
    19501 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
    19561 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
    19621 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
    19681 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
    19741 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
    19801 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
    19861 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
    19921 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
    19981 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
    20041 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
    20101 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
    20161 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
    20221 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
    20281 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
    20341 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
    20401 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
    20461 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
    20521 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
    20581 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
    20641 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
    20701 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
    20761 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
    20821 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
    20881 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    20941 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    21001 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    21061 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    21121 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    21181 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    21241 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    21301 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    21361 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    21421 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    21481 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    21541 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    21601 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    21661 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    21721 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    21781 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    21841 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    21901 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    21961 tacggacact cctacccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    22021 tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    22081 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    22141 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    22201 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    22261 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    22321 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    22381 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    22441 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    22501 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    22561 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    22621 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    22681 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    22741 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    22801 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    22861 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    22921 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    22981 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    23041 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    23101 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
    23161 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
    23221 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
    23281 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
    23341 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
    23401 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
    23461 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
    23521 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
    23581 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
    23641 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
    23701 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
    23761 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
    23821 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
    23881 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
    23941 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
    24001 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
    24061 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
    24121 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
    24181 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
    24241 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
    24301 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
    24361 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
    24421 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
    24481 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
    24541 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
    24601 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
    24661 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
    24721 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
    24781 atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt
    24841 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
    24901 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
    24961 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
    25021 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
    25081 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
    25141 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
    25201 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
    25261 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    25321 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    25381 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    25441 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    25501 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    25561 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    25621 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    25681 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    25741 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    25801 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    25861 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    25921 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    25981 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    26041 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    26101 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    26161 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    26221 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    26281 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    26341 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    26401 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    26461 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    26521 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    26581 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    26641 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    26701 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    26761 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    26821 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    26881 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    26941 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    27001 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    27061 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    27121 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    27181 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    27241 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    27301 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    27361 agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact
    27421 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    27481 aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    27541 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    27601 tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    27661 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    27721 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    27781 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    27841 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    27901 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    27961 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    28021 gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    28081 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    28141 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    28201 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    28261 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    28321 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    28381 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    28441 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    28501 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    28561 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    28621 gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca
    28681 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    28741 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    28801 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    28861 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    28921 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    28981 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc
    29041 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    29101 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    29161 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    29221 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    29281 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    29341 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    29401 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    29461 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    29521 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    29581 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    29641 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    29701 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    29761 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    29821 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    29881 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    29941 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    30001 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    30061 gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    30121 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    30181 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    30241 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    30301 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    30361 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    30421 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    30481 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    30541 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta
    30601 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    30661 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    30721 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    30781 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    30841 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    30901 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    30961 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    31021 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    31081 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    31141 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    31201 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    31261 attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    31321 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    31381 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    31441 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    31501 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    31561 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    31621 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    31681 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    31741 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    31801 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    31861 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    31921 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    31981 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    32041 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    32101 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    32161 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    32221 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    32281 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    32341 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    32401 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    32461 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    32521 ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa
    32581 aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc
    32641 taatagtttc gttttcaaca tttataagta aactttttct attggtaata tagattttcg
    32701 gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca
    32761 tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca
    32821 taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca
    32881 ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt
    32941 tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact
    33001 gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg
    33061 acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct
    33121 tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa
    33181 ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt
    33241 ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg
    33301 acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat
    33361 tgataaacag attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc
    33421 tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg
    33481 atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt
    33541 ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct
    33601 ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca
    33661 ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca
    33721 tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag
    33781 accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta
    33841 tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc atcgattaac
    33901 cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata
    33961 tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg
    34021 attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc
    34081 ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct
    34141 gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct
    34201 taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc
    34261 tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga
    34321 tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa
    34381 acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga
    34441 acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg
    34501 cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga
    34561 ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt
    34621 ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa
    34681 aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta
    34741 tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg
    34801 atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga
    34861 atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata
    34921 tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa
    34981 atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg
    35041 aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg
    35101 gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg
    35161 ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg
    35221 acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
    35281 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
    35341 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
    35401 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
    35461 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
    35521 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
    35581 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
    35641 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
    35701 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
    35761 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
    35821 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
    35881 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
    35941 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
    36001 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
    36061 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
    36121 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
    36181 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
    36241 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
    36301 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
    36361 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
    36421 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
    36481 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
    36541 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
    36601 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
    36661 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
    36721 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
    36781 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
    36841 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
    36901 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
    36961 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
    37021 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
    37081 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
    37141 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
    37201 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
    37261 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
    37321 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
    37381 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
    37441 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
    37501 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
    37561 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
    37621 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
    37681 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
    37741 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
    37801 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
    37861 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
    37921 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
    37981 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
    38041 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
    38101 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
    38161 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
    38221 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
    38281 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
    38341 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
    38401 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
    38461 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
    38521 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
    38581 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
    38641 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
    38701 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
    38761 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
    38821 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
    38881 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
    38941 tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt
    39001 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
    39061 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
    39121 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
    39181 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
    39241 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
    39301 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
    39361 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
    39421 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
    39481 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
    39541 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
    39601 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
    39661 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
    39721 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
    39781 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
    39841 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
    39901 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
    39961 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
    40021 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
    40081 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
    40141 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
    40201 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
    40261 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
    40321 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
    40381 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
    40441 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
    40501 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
    40561 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
    40621 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
    40681 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
    40741 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
    40801 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
    40861 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
    40921 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
    40981 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
    41041 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
    41101 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
    41161 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
    41221 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
    41281 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
    41341 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
    41401 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
    41461 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
    41521 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
    41581 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
    41641 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
    41701 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
    41761 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
    41821 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
    41881 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
    41941 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
    42001 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
    42061 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
    42121 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
    42181 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
    42241 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
    42301 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
    42361 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
    42421 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    42481 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    42541 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    42601 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    42661 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    42721 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    42781 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    42841 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    42901 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    42961 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    43021 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
    43081 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
    43141 taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc
    43201 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
    43261 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
    43321 agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
    43381 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
    43441 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
    43501 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
    43561 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
    43621 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
    43681 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
    43741 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
    43801 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
    43861 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
    43921 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
    43981 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
    44041 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
    44101 cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc
    44161 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
    44221 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
    44281 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
    44341 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
    44401 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
    44461 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
    44521 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
    44581 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
    44641 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc
    44701 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
    44761 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
    44821 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
    44881 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
    44941 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
    45001 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
    45061 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
    45121 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
    45181 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
    45241 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
    45301 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
    45361 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
    45421 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
    45481 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
    45541 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
    45601 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
    45661 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
    45721 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
    45781 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
    45841 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
    45901 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
    45961 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
    46021 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
    46081 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
    46141 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
    46201 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
    46261 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
    46321 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
    46381 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
    46441 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
    46501 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
    46561 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
    46621 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
    46681 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
    46741 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
    46801 aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa
    46861 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
    46921 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
    46981 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
    47041 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
    47101 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
    47161 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
    47221 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
    47281 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
    47341 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
    47401 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
    47461 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
    47521 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
    47581 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
    47641 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
    47701 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
    47761 acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg
    47821 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
    47881 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
    47941 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
    48001 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
    48061 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
    48121 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
    48181 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
    48241 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
    48301 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
    48361 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
    48421 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
    48481 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
    48541 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
    48601 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
    48661 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
    48721 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
    48781 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
    48841 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
    48901 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
    48961 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
    49021 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
    49081 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
    49141 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
    49201 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
    49261 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
    49321 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
    49381 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
    49441 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
    49501 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
    49561 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
    49621 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
    49681 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
    49741 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
    49801 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
    49861 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
    49921 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
    49981 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
    50041 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
    50101 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
    50161 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
    50221 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
    50281 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
    50341 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
    50401 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
    50461 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
    50521 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
    50581 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
    50641 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
    50701 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
    50761 aataaagaat ag
//
LOCUS       DAFBYI010000006        47025 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFBYI010000006 DAFBYI010000000
VERSION     DAFBYI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.006"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            14..637
                     /locus_tag="LLD04_000282"
     CDS             14..637
                     /locus_tag="LLD04_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647243.1"
                     /translation="MKFTNIKKYRAFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILT
                     ALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIA
                     TLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYI
                     LYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(877..960)
                     /locus_tag="LLD04_000283"
     tRNA            complement(877..960)
                     /locus_tag="LLD04_000283"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Leu,seq:gag)
     gene            1015..1194
                     /locus_tag="LLD04_000284"
     CDS             1015..1194
                     /locus_tag="LLD04_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647244.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1343..2560
                     /locus_tag="LLD04_000285"
     CDS             1343..2560
                     /locus_tag="LLD04_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5647245.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2744..3580
                     /locus_tag="LLD04_000286"
     CDS             2744..3580
                     /locus_tag="LLD04_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5647246.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3656..4804
                     /gene="nagA"
                     /locus_tag="LLD04_000287"
     CDS             3656..4804
                     /gene="nagA"
                     /locus_tag="LLD04_000287"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK5647247.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5025..6083
                     /locus_tag="LLD04_000288"
     CDS             5025..6083
                     /locus_tag="LLD04_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK5647248.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6087..7121
                     /locus_tag="LLD04_000289"
     CDS             6087..7121
                     /locus_tag="LLD04_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK5647249.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7223..7939)
                     /locus_tag="LLD04_000290"
     CDS             complement(7223..7939)
                     /locus_tag="LLD04_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5647250.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7908..8693)
                     /locus_tag="LLD04_000291"
     CDS             complement(7908..8693)
                     /locus_tag="LLD04_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK5647251.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8684..9433)
                     /locus_tag="LLD04_000292"
     CDS             complement(8684..9433)
                     /locus_tag="LLD04_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5647252.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9731..10255
                     /locus_tag="LLD04_000293"
     CDS             9731..10255
                     /locus_tag="LLD04_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK5647253.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10271..11770
                     /locus_tag="LLD04_000294"
     CDS             10271..11770
                     /locus_tag="LLD04_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK5647254.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11858..12460)
                     /locus_tag="LLD04_000295"
     CDS             complement(11858..12460)
                     /locus_tag="LLD04_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5647255.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12564..13520
                     /locus_tag="LLD04_000296"
     CDS             12564..13520
                     /locus_tag="LLD04_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK5647256.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13825..14781)
                     /locus_tag="LLD04_000297"
     CDS             complement(13825..14781)
                     /locus_tag="LLD04_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5647257.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14955..15515
                     /locus_tag="LLD04_000298"
     CDS             14955..15515
                     /locus_tag="LLD04_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647258.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15508..16332
                     /locus_tag="LLD04_000299"
     CDS             15508..16332
                     /locus_tag="LLD04_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK5647259.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16342..16959
                     /locus_tag="LLD04_000300"
     CDS             16342..16959
                     /locus_tag="LLD04_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5647260.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16977..17660
                     /locus_tag="LLD04_000301"
     CDS             16977..17660
                     /locus_tag="LLD04_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5647261.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18343..19626
                     /locus_tag="LLD04_000302"
     CDS             18343..19626
                     /locus_tag="LLD04_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5647262.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19642..20529
                     /locus_tag="LLD04_000303"
     CDS             19642..20529
                     /locus_tag="LLD04_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647263.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20546..21382
                     /locus_tag="LLD04_000304"
     CDS             20546..21382
                     /locus_tag="LLD04_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5647264.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21385..22008
                     /locus_tag="LLD04_000305"
     CDS             21385..22008
                     /locus_tag="LLD04_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647265.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22005..23297
                     /locus_tag="LLD04_000306"
     CDS             22005..23297
                     /locus_tag="LLD04_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5647266.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23656..23753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23869..24450
                     /locus_tag="LLD04_000307"
     CDS             23869..24450
                     /locus_tag="LLD04_000307"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK5647267.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24453..25760
                     /locus_tag="LLD04_000308"
     CDS             24453..25760
                     /locus_tag="LLD04_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK5647268.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26176..26664
                     /gene="purE"
                     /locus_tag="LLD04_000309"
     CDS             26176..26664
                     /gene="purE"
                     /locus_tag="LLD04_000309"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK5647269.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26657..27781
                     /gene="purK"
                     /locus_tag="LLD04_000310"
     CDS             26657..27781
                     /gene="purK"
                     /locus_tag="LLD04_000310"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK5647270.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27794..29089
                     /gene="purB"
                     /locus_tag="LLD04_000311"
     CDS             27794..29089
                     /gene="purB"
                     /locus_tag="LLD04_000311"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK5647271.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29269..29646)
                     /locus_tag="LLD04_000312"
     CDS             complement(29269..29646)
                     /locus_tag="LLD04_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647272.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29902..30630
                     /locus_tag="LLD04_000313"
     CDS             29902..30630
                     /locus_tag="LLD04_000313"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK5647273.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30630..30884
                     /gene="purS"
                     /locus_tag="LLD04_000314"
     CDS             30630..30884
                     /gene="purS"
                     /locus_tag="LLD04_000314"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK5647274.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30886..31557
                     /gene="purQ"
                     /locus_tag="LLD04_000315"
     CDS             30886..31557
                     /gene="purQ"
                     /locus_tag="LLD04_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK5647275.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31558..33780
                     /gene="purL"
                     /locus_tag="LLD04_000316"
     CDS             31558..33780
                     /gene="purL"
                     /locus_tag="LLD04_000316"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK5647276.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33765..35204
                     /locus_tag="LLD04_000317"
     CDS             33765..35204
                     /locus_tag="LLD04_000317"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK5647277.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35227..36279
                     /locus_tag="LLD04_000318"
     CDS             35227..36279
                     /locus_tag="LLD04_000318"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK5647278.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36276..36854
                     /gene="purN"
                     /locus_tag="LLD04_000319"
     CDS             36276..36854
                     /gene="purN"
                     /locus_tag="LLD04_000319"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK5647279.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36851..38386
                     /gene="purH"
                     /locus_tag="LLD04_000320"
     CDS             36851..38386
                     /gene="purH"
                     /locus_tag="LLD04_000320"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK5647280.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38402..39676
                     /gene="purD"
                     /locus_tag="LLD04_000321"
     CDS             38402..39676
                     /gene="purD"
                     /locus_tag="LLD04_000321"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK5647281.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39798..40712
                     /locus_tag="LLD04_000322"
     CDS             39798..40712
                     /locus_tag="LLD04_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5647282.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40845..42410)
                     /locus_tag="LLD04_000323"
     CDS             complement(40845..42410)
                     /locus_tag="LLD04_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5647283.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42403..43854)
                     /locus_tag="LLD04_000324"
     CDS             complement(42403..43854)
                     /locus_tag="LLD04_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5647284.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43868..44884)
                     /locus_tag="LLD04_000325"
     CDS             complement(43868..44884)
                     /locus_tag="LLD04_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5647285.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45104..46192
                     /locus_tag="LLD04_000326"
     CDS             45104..46192
                     /locus_tag="LLD04_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK5647286.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46246..>47025
                     /locus_tag="LLD04_000327"
     CDS             46246..>47025
                     /locus_tag="LLD04_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647287.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYF"
BASE COUNT        14981 a         8053 c        10257 g        13734 t
ORIGIN      
        1 aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagcctttt ttttagacat
       61 gaatatctta tttttattgc tatttttctt aattttcctt ctaacatata ggaaatgtca
      121 aaactattat gtgcgtatat tgactgcatt accgtttttc ttaaacctga ttatttattt
      181 tggtaataca atgggtcaaa gctttactta cgtcaatgga aataaaaggg caatgatttg
      241 gagtagtagt aacctgaata atctctttac agagttagga acgaaactgt cactttttta
      301 tccaggtact tggatagcta ctttagtagt cttggcatta ttactatgtt taattgttgg
      361 aatttattta agttttgaca ataagaaaac ttctattttt ttagttattt tgatgataat
      421 gggattttgt tcaaggttaa ttatgggatt ttctcccaca gtatgggctt ccggaatgcg
      481 gacgtactat atactttatg ttgttatcgc catcttagta ctaatggcag ttaaagagct
      541 aatgaaaagc atgagtgtac aaaaaaatga atttatgcaa tttggattaa ccgtgcttgg
      601 tatttgtacc tttataataa cggtgataaa cagataatat acaaaaaatc cctcgcttta
      661 gtattaggtg agggatttta ctcgtttttc gattaattgt tttaacactt gcaagtttct
      721 acgagtagct tgtttccgac cttcatatac tcacctcagg ctgtttacat gattgtacgt
      781 tctttttgta aaggatacag tgatatgtat ataatggcat aaagaaacgt tcaaacaaaa
      841 aaactcgtga agcaaaatta acttcacgag ttgtagtgca gatgatggga ctcgaaccca
      901 cacgagctaa cgctcacatg accctcaatc atgcttgtct gccaattcca acacatctgc
      961 gtaataataa cacactaatg atagcgaggt tttacatagt tgtcaagctc aaaaatggga
     1021 ggacttatta agatggagaa atatgacgga gaattttcag gactgggaat gatcttaggt
     1081 gtattgattg gacttgcatt cggacgtttt ttgcttggtt ttatgctagg aatcatttgt
     1141 ggaattgcaa tggattgggc agcgaatcta tggaatgact atcatgacaa ttaagttctc
     1201 aacgcttgtt tgtgtttttt taatacttac gtttttccta aataagtggc aaaattttct
     1261 aggatggagt atgatattga aatagtaaat aacaaaaaat ttggaaagtt ctaacttttt
     1321 gaaatgaggg attggctaag tgatgagaga atttgaaaaa tccaacaaat tagaaggtgt
     1381 cagttacgat gttcgcggtc ccgttctaga agaagcggag cggatgcaag aagagggcat
     1441 tagtatttta aaattgaaca cgggaaatcc cgcacctttt ggatttgaag caccaaacga
     1501 agtcattcgt gatttgatca tgaatgcccg tgactcagaa ggctactcag attccaaggg
     1561 gatcttttct gctagaaaag caattgagca atattgccaa ctgaaacatt ttccgaatgt
     1621 gacgatcaat gatatctaca ccggcaacgg agtgagcgaa ttgatcacga tgtgtatgca
     1681 ggggttgctg gacaatggag atgaagtact ggttccaatg ccggactatc cgttgtggac
     1741 ggcttctatt gcattagctg gaggcacacc tgttcattat atttgcgatg aagaggcaga
     1801 atggtatcct gatattgatg atatcaaatc aaaaataacg agtcggacaa aagctattgt
     1861 tatcattaat cctaataatc ctactggggc actttatcca aaagaattat tagaagaaat
     1921 cgttgaagta gctcgacaaa ataacctgat tatttattcc gacgaaatct atgatcgctt
     1981 agtgatggat ggtctagaac atattccgat tgctacatta gcgcctgatt tgttcgttgt
     2041 tacgctgaac ggtctatcca aatctcatcg tgtagcagga tttcgagtag gctggatggt
     2101 cctaagtgga gataagtcac acgtcaaagg ctatatagaa gggctgaata tgttgtcctc
     2161 catgcgtttg tgttccaatg tattgtctca gcaaattatc caaaccgcgc taggcggtta
     2221 tcaaagtgtc gatgagctgc tcctgccagg cggaaggatc tatgaacaaa gagaatatat
     2281 ctataatgca atcaatgata ttcctggtct ttcagccgta aaaccaaaag ctgcttttta
     2341 tatattccca aaaattgata caaagcgatt caatattttg gatgatgaaa aattcgtttt
     2401 ggacttttta catgaacatc atattctact tgtacatggt ggtggattta attggaacca
     2461 gccagatcat tttcgaatcg tttatcttcc taaaatggat gatttgaaac aaacagctaa
     2521 gaaaatgaga gaattcctag caacctatcg ccaaaaatga ttttaaaaac caaatttttc
     2581 agtaggaggg ccacagacaa acacactgag gctctctttc tagtggagtt ttcttaaata
     2641 actaaagtat attatttagt tgcaagtctt ttcttgaagt ttgcgacttc ctaaagtaaa
     2701 atacgaatag aatgcgaata cgcatatttt ggggaggata cttatggaaa cgttaatcga
     2761 gtcctttgga acgatcatta aagagattcg aaaagaacaa aaaatgacgc aaaaaatgct
     2821 ttcgcatgat atttgttcac aaagtgtatt aagtcggatc gaaaataatg aggaactgcc
     2881 gaatgtattg gtgatgatgc agatttgtca acggctaggg gtaacgattg atcatgtgat
     2941 gaatctctct cgaaaaacta ctcggacaaa cgataaatta tttgaactgt tggatagttg
     3001 tttccaaaaa cgtgattatc ggaagttaga acaagtacta aaatcttctg atatttctga
     3061 aaatctgtat caagcaactg actttcaacg ctattattat tatctcggtg tttgcgaatt
     3121 cacgttgcgt caaaatatct ctcagtccct ttattatttg aaagaagcat taagctactc
     3181 tagccaaaaa gaaagaatcc atgtttctga cacagaaata caactgatta gttgtatcgg
     3241 aaaaatatat ggtgtggcag gaaaaacggc tgaagcacgc tactatttga gtcgaagcat
     3301 ccaactgctg catcaatcag ctgatgaacg atcaaaaagt gaattttcgc aaatttttta
     3361 taattatgga aatttcttat tccatcaaaa tgaaatcgac gaagcgttag aacaggtgaa
     3421 tcaaggaatc tattgggctc aagagaagaa tagctactat tacctcaatg atttatttgt
     3481 gcttaaaagc ttaatataca agcgtaaaga agaaccagaa agagcagcat tctatgaagg
     3541 cttagcacaa gctgtgaaac aaatttcaaa aaatttgtaa atggtgtaga ccattcttga
     3601 cttcttctct aaataagagt actttagagg tagaaaaaca aagggagaga atagaatgag
     3661 aacttttatc tatgctgaca aattcttttt agcgtcaggt gtcaaacatg caggctatct
     3721 tgaaattacc gatggacttt ttggtgaata caccaaagaa ctacctgagg gagaagtcaa
     3781 catcattgac caaagcggca aatggattgc tcctggtttg gtagatacac atattcacgg
     3841 ctatatgaat catgatgtaa tggataatga tgcagaaggc atcaaagtga tgtctgaagg
     3901 tctgttatct tgtggagtga cttcattttt accaacaaca ttgacgtcaa gcaaagaacg
     3961 tttaaaagat gtagctgaga caatcggcaa aatgtatcag gaagcaccag gagctaaaat
     4021 caaaggaatc tattttgaag gtcctttctt cacagaagaa cacaaagggg cacaaaaccc
     4081 aagttatttc ggggatcctg atttggatac tttccatgaa tggcaagaag cttcaggtgg
     4141 tttaatcaaa aaaattgctt tagcaccgga acgtcaaggt gtagaagagt ttgtcaaaca
     4201 agtgactgaa gaaggagtag ttgtttctct aggacacagt aatgctacac tagaagaagc
     4261 gcaaaaagct gttgaagcag gcgcaagtgt atttgtccat gctttcaatg gtatgcgtgg
     4321 attgaatcat cgcgaaccag gtatggtggg tgcgttatta tctttagaac atgtcttttc
     4381 tgaattgatt tgtgatggcc atcatgtaca tccaaatgct gcgggaatcc taatggaaaa
     4441 agcaggacat gatcatgtcg cattaattac agactgtatg atggccggtg ggatgcctga
     4501 tggaaattac aatttaggcg aatttccagt agtagtaaaa gaaggaacag cacgtctaga
     4561 atctggcaac ttagctggaa gtatattgaa attgaaagaa gcaattaaaa atgttgtaga
     4621 ctggggatta gcaacaccag aacaagcagt catgatggct agtctgattc cagcaatcag
     4681 ctgtaaaatc gatgatacat gtggaaaaat cgctcaagga cgcgatgcag actttatcgt
     4741 attgaaccct gatatgacat tggaagcaac ttatctagat ggcgtggaac gtttccacgc
     4801 ataatataat ttaagttaac cttaaaacta aacttcttaa gtttggtttt aaggtttttt
     4861 ttgtgttcta gtaaaaatca ctttacataa gtttcaaaag tttctttaat ttcctattgt
     4921 tcaataagta acaatgtaaa gataaaatta aaaatgttaa tacgtattag caaaataaaa
     4981 gcttttttta ttgtgaataa atgaacataa ttaggaggag aaagatgaac ttatttgaca
     5041 tttttcgatc gactttgaca gatatgtcga ttgtcagtgc aatcacttca acagttttta
     5101 ttatattatt agggtttgtt tgtagaaaaa aggggatttt ttctgaatct gttggaaaaa
     5161 ttctatcgaa agtcgtctta acagtggctt tgccagcact ggcatttcga gctttcatgc
     5221 aagatatcaa tacagaatta ctgatgcaag gaatcaatgt cttgatttgg ggattagtta
     5281 tttatattat tcttattcta gtttcaaaac cttttttctt aagctataaa ggggataagc
     5341 aaacaacgtt gcgtgtcctg acgatctttg gttctacgac ctttttcgga acgcctatcg
     5401 tcagtgcgat ttatggtcct gtcggcgtga tgtattcctc aattttcaat attggctacc
     5461 gaatcttttt gtattcttat ggatacataa aaatgagtgg cttgaagatg gaagctaaaa
     5521 atatcaaagc gatgttcgtc aatccaatca ttatcgctac ttttgccggc cttttcattt
     5581 ggctgttcca agggtatctg ccacaagtgt cagttacagg tgcagaagga gagataagcc
     5641 aagtagcgtt tttgcgaatt gaccagactg caccgtggct atttcagccg atggattatt
     5701 tagctggttt aagttcacca ttggcgtggc tagcaatcgg cgcaacatta ggtgaagtaa
     5761 gcttcaaaaa agctgcatct gaaaaaactt catggtatta cagtctagtc aaagtggtat
     5821 ttgtacctgc attgaatatc gtgattctag cagttttgac ggcaacgaat attctaccag
     5881 tcaattttga agcactggca acaatcgtta tcatgatggc tacaccagct gcaacagtgg
     5941 cagcagccta tgctatcagt tttgataggg aggcactttt agcctccaat gcatccttac
     6001 tgtcaactat ttttgctgtg gtactgacac ctgtatggat cgttgtatta caaatgattc
     6061 atggcatagg tttattttca taagaaatgg aggaagaaag tatgagaatc gcatgttacg
     6121 gtgttcgtcc gaacgagata gatttttttg aaaaattgaa tatttaccat tatgatctca
     6181 gtctttatga agaactgttg acacatgaca atatcgtgtc agcaaaggac catgatgctg
     6241 tattgttgag gggaaattgt atagcagatg agaccaatct tgcaaaaatg caggaatatg
     6301 ggattcgtta tgtttttaca cggactgtgg gagtcaatca tatcgatcta caagcggcag
     6361 ctgattttgg tatgacagta gcccgtgtac catcgtattc gccaaatgcg atagcagagt
     6421 tatctttgac ttttgccatg atgcttttga gaaatactgc gtatacgacg atccgaacat
     6481 ctttcaaaga ttttcgagtg gatgagcaaa tgtttagtcg tgaaatcaga aattgcacag
     6541 tagggatcat aggaacaggc aggatagggt tgacggaagc tgttttgttt aagggactag
     6601 gagccaaagt gatcggctac gatattcatc agacagaagc tgctaaaaaa gtattgacgt
     6661 tccactcgct tgaagagtta ttagccaaaa gcgatattgt cagtattcat gtacctcata
     6721 tcccaggtga aaacgatcaa atgatcaatg aaggattttt agctcaaatg aaaaaaggta
     6781 gcatcttgat taatacagcc cgtggagagc ttcaagataa tgaagctatt ttaagagctt
     6841 tgaaaagcaa tcatttagct ggttttgcaa ccgatgtttt tgcaaatgaa acagaggttt
     6901 ttttccgatc cttcaagcca tgggagacga ttccagaccc tgctatccag caattagtag
     6961 aaatgtaccc tagagttctt gtaaccccgc atgtaggttc aaatacagat gaggcattat
     7021 caaatatgat ttcgacaagt ttcgagaact ttcgtgaaat aatagaaact ggaaaaacga
     7081 aaaatgaggt atctcttcct aaagcccgtc aattaagata agaaaacatg gatattcgtc
     7141 aataaaatgt ttaaaaatag tccaagtaaa cagttgttac ttctgtatac ttggactatt
     7201 ttgtatggga taattcgatg actcaacagt aatctgtgag ctttttccgc ccattctttg
     7261 tcaattcata acaggttagc ggccggccga tttccaaata tttgattctt tcgttcaata
     7321 tatcttgttc tactaaaaaa ttcaaatatt tcttgacgga tatacgtgac agtgagcttt
     7381 tttgagccag ttcttttgtt gaaaaaatag tatcttcttt tagaataaat gcacttattt
     7441 tttttaaagt cagttttgac agacctttag gcaaatcgat gaatgatggt atttcgtgaa
     7501 tgggagtggt gtggttgaat acttgatcga tcaatttctg atcggctttt tcagtcgttt
     7561 ggattgtctg agagaaagca agatactttt cgatcgccag ctgaaaacgt tgcaaagtga
     7621 acggtttgat caaataatct aaggcaccgt agcggatcac attctttatg gtttcgatat
     7681 catctgcagc agtgatcaaa atcactggga tatcatttcc ttgctgtcta agccaagata
     7741 aaaaatggat accactttcg ccaggcatgt atacatcaag cagcagcaga tctgcgtttt
     7801 tttgtctgaa atattttttt ccttcttcta catttctgaa actttcgatt acgttcatat
     7861 tagggatgct ttccacaaaa gtacgattca attctgcaac cattggatca tcttcgatta
     7921 tcaggacatt tgtcatcatt ttgatcttcc ttttttggta attctagata gacgattgta
     7981 ccgccatttg tttgtttgga aaattctaaa aatccaccgt ggcgtttgac gattgaatgg
     8041 atgagattca atccaaaccc acgattgatt cccttcgtcg aaaaacctag cgtaaatatt
     8101 ttttctgaga tttcttctga tatcccgaca ccgtcatcgc agacggtcac gatgaatact
     8161 tcagcttctg tgtcatagtt taaatgaatc cggacctgtc cgtttctttc atgtttttgt
     8221 agcacggctt cttttgcatt atcaagtaag ttccctaaca cttgcaaaag tgaatggatg
     8281 gagtcatcca tctccatttt agggataaaa gaatcgcttt ctaacagcat ttcaacagat
     8341 tgttctttgg cttcgtttat tttgcctaat aaaaaaccgg caattgcagg tactttgatc
     8401 agacttgaga cttggccaat ctctttttta taattttgat ttagctgctg gataaaatgg
     8461 gtgacttctt catacttctt caaatcaatc aaaccagaaa tgacatgaag tttattcatg
     8521 aattcatggg tttgagctct catggagttt atatattgtt tcgtcccccc aagctcctcc
     8581 attaatttca ttacttctga tcgatttcgt aaagttgcga ctgctcctaa aaaacggtca
     8641 ttgctaaaaa gaggtgtaac gctagaaata gtttctatcc catttaaata catggattga
     8701 gtccgaacgg tcgtttgatg ctcaatcgtc tctttaaaca aaacgtcata tagattatca
     8761 ctgatttttt tcctacttcg aaatccaata gctgctctgt ttggtagaaa atgttttttg
     8821 caggtgtatt gatcaagagg atagttttat cagaagagat ggctaagatt ccttcatcta
     8881 cttcattttc gataatcgtt ttttcactta actgttctgc tatttcttgt ggttcaagtc
     8941 caagcagtac ttttttcatt ttttgggcaa gaaaaacggc gcctatgatg cctacgctaa
     9001 gtccaataat caatgaaaaa acaatcattt tttgtccatc tttgacttga ttgtcaatcg
     9061 tgttgtttgt taagccaaca caaactgcac caataatctc ttgatcataa taaataggga
     9121 cgaaatagcg gaagccgaac cctaacattc cttctttttc agagaaaaat tcttgtcctc
     9181 caatcacttt tttcacatct tcatgacttg agaaaggaag accgatgtaa cttgggtctg
     9241 cgtgagaata tcgaatgcct tcagggtcca tgacaacgat aaaatctaaa ttcgatgcaa
     9301 tcatgatttc atttgttatt ccttgaatcg catgtgtcgg gtcaccagtc tgcagctttt
     9361 tttggatgag tttatcattg gcaacgattt tagcaatgct agagatttta tctgacacac
     9421 tagatcggtt catcgaaaga ataaaattcc tgatcaatat ggcagaaaca atcaaagaaa
     9481 cgaatgtgga gaaacaaacg acaaggatga tcgtgttctg gagctttaat ggttttttct
     9541 taaaaaaagg aaacataggc tacctcgaaa gaatttattg tgaaaatttt aacatgaaaa
     9601 caaaaattta acaaattgtc aacgataaac acaaccttcc tataaaaaac accactgagt
     9661 cggtaaaaga agctatacag aattttcaaa cattggtatg atagacgtag aaatggaagg
     9721 aggcgtgtat atgatagggg gaatcgcact tgagacgatt tattggtata cattgctgat
     9781 ttgtgcagga ttggcagtcc tattgattat ttttggagat gtattcgatt ttgatggccc
     9841 gcttgatccg atgcttatta tcccttggtt aactttcaca agcttatttg gttttctagg
     9901 tgagcgttta acagatcaat catctttatt tttatttatt gctagtggag gcatatcgat
     9961 agtgttggta tttttgctta atttctatat attgatgccg atgaaacatg ccgaatcaac
    10021 gatgtcgtct tctgaaaaaa ctctggaagg acaagtagct gttgtgatca caccgatccc
    10081 tgtacgaggt atgggagaaa ttcagttgaa aagtgttacc ggttccatta gtcgtccagc
    10141 ttgtttttac agcccccaag aacagacagc tgagcgggga acgaaagtat tgatcattga
    10201 gatcaaggaa agaatctgtt atgtcatgcc ttatgaaagc atgataaaag cttaaaggag
    10261 cgtgtgttaa atggaaatac cagttgtttt tatcgcgatc gcaatcgcag ctttgatttt
    10321 actgatgttg ctgatcgtct ttgtagcgaa gtaccagaca gcaaaaccgg atgaagcgtt
    10381 gatcatcagt ggaagttatt taggaagcaa aaatgtgcac attgatgaaa gcaataacaa
    10441 aatcaaaatt gtgcgtggag gtggcgcatt cgtcttgcca gtttttcaac gttcgaatcg
    10501 gatcagtcta ttatcgagta aattggacgt ctccacccct gaagtgtata cggaacaagg
    10561 cgtaccggtg atgtgtgatg gtacttcgat catcaaaatc ggttcatctg tagaagagat
    10621 tgctacagca gcagaacagt ttttaggcaa gacaagagaa gaactggaaa atgaagcaag
    10681 agaagtatta gaaggacact tgcgctcgat tcttggttct atgactgtag aagaaatcta
    10741 tcaaaatcgc gataagttca gtcaaagcgt acaagaagtc gcttctgtag accttgcaaa
    10801 aatggggttg atcatcgttt ctttcactat caaagaggtc agagacaaaa acggttatct
    10861 tgactcttta gggaaaccaa gaatcgccca agtcaaaaga gatgcagata tagcagaagc
    10921 ggaagctcta aaagaaacgc gtatcaaaaa agctcaagca gaaaaagaat cacaagcagc
    10981 tgaattacag cgacaaacag agatcgcaga atcattaaaa gaaaaagaac tgaaattagc
    11041 aacctacaaa caagaacaag atgttgctaa agcaaaagca gaccaagcct acaatttaga
    11101 aagtgcccga gcacaacaac aagtaatcga acaggaaatg cagattaaaa tcgtagaacg
    11161 tcaaaaacaa atcgaactgg aagaaaaaga aatcacacgt cgtgaaaagc aatatgattc
    11221 ggaagtgaag aaaaaagcag atgccgatcg gtacgctaaa gaacaagaag cacaagctca
    11281 aaaagtcaaa gaagtgactg aagccgaagc agaacgattc cgtgtggaag cactggcaga
    11341 agcggaagcc aataagacac gtttagctgg acaagcagaa gccgaagcag cactagcaaa
    11401 aggaaaagca gaagccgaag caaaacaaaa gatcgccaat gccttcaagg aatatggcga
    11461 agcagctgtc ttgagtatgg ttatcgatat gctaccgcaa ttgatgcgcg aagcagcaca
    11521 gccgctagga aatatcgaaa aaatctctgt tgtcgataca ggaagcagtt caggagaaac
    11581 cagtggagcg aaccgagtga caaattatgc cacaaatttg ctttcttcta ctcaagaaac
    11641 actaaaagcg acaacaggac ttgacttgaa ggaaatcgta gagaatttgt cgacacgtgg
    11701 gactactagc caagtaaata tgtatgaagc gccaaaagag gcagaacaaa actcgatgaa
    11761 ggaagaataa agtagtacac ttcaccgttc aatcaaaaat ggaggaagac tttttaaagt
    11821 tcttctctcc atttttttat tttattgcaa aagaaaatta gtcctcgcta tttttctttt
    11881 gtttttggat cttagcttct atctcttgta aatttgttct tagcaatcgt tctttctttt
    11941 ggagttcgac caaatcttct gatagttctt ccttcttttc tgtttcagga ggacgaagaa
    12001 agaaattgat accgattgca atgaatgtcc ctataaaagt atctagtaca cgttggatag
    12061 catacaaagc ggattcgcct tgagggatgc tcaacgcaat cagcaacaac gtagctattg
    12121 cggaaatgat accggaatta ttgttaatac catctgacac gacgatcaca ataataacta
    12181 gaataggaag caagatcaat tctaccaaaa aatcattgtg aaaatagctt ttgacaaaaa
    12241 aataaatgat agctaatgta ccacctaatg tattgccgat gatcctagac tttccaaaag
    12301 aaacggttgt agtcaagtcc tgacgcaaag aaaaaacagc agataatgca gcaatcatcg
    12361 ggattcctcg ttgagtcaaa tgaaaaaaca agatacataa aaaaacagct aaggcacttt
    12421 tgatcgtacg catacctagt cgaaaacggc caaattgcat agagattctc ctttttggtc
    12481 tttttactat catacctaaa cttttgtctg atagctagtt tttcattatt caagtataat
    12541 taaaataaaa aagggggaag taaatgcgta taggaatgcg aacagtcaaa acagtgatca
    12601 gtgtcttctt ttcgatggtc acagcgggta gcttatcgct gttgtattgg ccagctgcag
    12661 ggatcattgc attattaagt gtaggaaata ccaagcgctc tacattgcgt acgggcatga
    12721 atcgaatcgc agcctttata ttagctacgg caacagcact gttcagtttt tttgctgttg
    12781 ggtatactat ttttggcttc agtctttttt tattcctgtt catcccgctt gcctctcgct
    12841 tcaaattaac cgaagggata gtagtaaatt cggttttggt cactcattat ttagtggagg
    12901 aaaacatgtc ttggcagctg atcggcaatg aagcgggatt aatggcgatt ggattagtat
    12961 ttgctttgct tgcaaatgtt tacatgccag atcgtacgaa gcagctgaag gaaaaccaaa
    13021 tacagatcga aaaagaattt cgaaatattt tgagacaaat ggcagaattt cttttatcag
    13081 aaaataaaga agatgtgcag atcaaatgcg aacatttact gacattcatt cgcgaaagcc
    13141 aagaagatgc cagagaacat caagaaaact actggctcag acagccgcta tactacgaaa
    13201 cttacttctc tatgagaaga gcacaggcca atgtgattaa agacatgctg gaaaatctag
    13261 agagaatcca gcaaccagct tattatggaa aacatatcta tggtttattg atttatactg
    13321 cagaaacatt ttcagagtca aatgatggca gacaaatttt aacacgtatt gaagaagttt
    13381 atgtattgta tcggcaaatg cctctgccga ctagtcgccc tgaattcgag gatcgagcag
    13441 aattgttcca gtttttgcag tcttttaaaa gttttattga aatcaaggca gaattctccc
    13501 aacaaaaaat ccaaaaatag aggttgtgaa ttcagctaat attcaggtca aaaagctgat
    13561 ttcacaacct cttctttaaa cggtgttctc ctgtcaactc ctcggtcaca agtcacaata
    13621 ttcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcgga gctgaacggt
    13681 aggctcacaa cctctttctt aaacggtgtt caaaagcttt cctctgcggt aataagtcca
    13741 gccaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
    13801 cagctttttc acaacctttt aaaattacat atttttccat agaacttgca tttgatccaa
    13861 gatttcgatg acatcttttg tcaagaagag agagcgattg ggcaagtcac cttcaatcat
    13921 gtgtaccata ttctttattt cgtaattcat tgcttcagat gtattcccgc tttcgatgaa
    13981 ttcctttgta ccatctgtaa ataagatctc cgcacgatca gcacgcgggt aatcagcaat
    14041 cgtcacataa ccgttctcaa aggcaacgat accgactttt ggcatttttg cctgaaaagt
    14101 caagctgaca gcagcaagtt cattttcttt gttacgcaag atagtgacgg attgttcgtc
    14161 cacgcctgtt tcaaaaggaa ccattgtaga ggcaactact tcaggttgag agctcaaaaa
    14221 gaaacgagcg aaagatacag catacgtacc gatatccaac agagctcctc cagctaattc
    14281 tggattgaag aaccggtttt tagggtctgg ttctttgtaa ctgccgaaag gtgcttggat
    14341 catttttaaa gcccccagtt ttcctgtatc cattattgag cgtaactgct gataaagtgg
    14401 catgttgaag attgtcatcg cttctgctaa gatcaaattc ttttcttctg ctaagcgcat
    14461 agcgtcagcc agttcttttt tattcatcgt gatggctttt tcacatagaa cgtgtttgcc
    14521 tgcttccaat gctgccaaga tatggtcgat atgctgtcta tttggtacag caatgtacac
    14581 gatgtcgatt tcagcatcgt ctaacatttc ttgatagctt ccgtaagctt ttggaatatt
    14641 gtaacggtga gcgaagtttt ccgctttttc tgaagttctg gaagcaactg ctgttagttc
    14701 gctcgtctct tgattgaagc tttcggcaaa ctcatgtgcg atggtaccaa gaccaacgat
    14761 tccccattga tattttttca tctgaaaacg ctccttcata gttggattga gtttcttgtc
    14821 tgtctattat tataacaaaa atcacgaaga ataaagagga gaggacttga aacattaaaa
    14881 aaaatgaaag cagcgattta agaggtgctt tttgatcaaa acttaagtag aatagttaaa
    14941 aggaggggat aaacgtgcat caagtatata ttgatcttat tatgagtgta ttattagaac
    15001 agtttgaaac tgaaaaaaat tttttagatg aatacttgct gatcagtcca gaacaatggc
    15061 aaaaatggaa agatggcgag aaaaatctgc catctgaagc gatgcagaaa gtaaaaaatc
    15121 ttttcactga ttatgaatgg atgctaacgc aaaagatttt gcgtcaaacg attatttttc
    15181 ccgaaaaacg aaacgttgcc gttgcagagt acaagcaaat caaaacacgc atcgcgcaaa
    15241 aatggcttag cagcaaagca gcaaaagtcg agctgatccc tttgaaaaat aatcagcagg
    15301 ctggtagata ccttgacttg aaagtaagta ttcaatatga tgaatggggc tatgatgata
    15361 ttttgaattt tcgtctgcca gcagatgttc aaaaacagat cgaaggagaa aaaatcgaac
    15421 ttctcgattg ggtgaatgaa aatctggaag aaacttacgt tcatgaggcg aatggggaac
    15481 gtgaagaaca acgaaaggat ttttcaagtg aataaatttt taggatttgg agcagttgtg
    15541 attatgatcg cagcaatggg ctatgttatt tttagcgatg atgattcgca tgttcaagcc
    15601 ggcaatcaag cagtcgaacg aacaagcacc aaaacaactg ctagtaaaga aaagaacagt
    15661 caaacaacca gcagcaaggt aaaggacaaa gaaacaaaag atcgtacgtc caaacaattg
    15721 ccagatgtta aaacagacga ttggaagcta gttttggttg gtccagatca caaattagaa
    15781 aaagaaattg atgagtctac tcaattggca tcattaagta acggatactt gatcgacaaa
    15841 cgcattgctc cagaatatga agcatttgcc aaggctgctg aagcagctgg atttccatta
    15901 gtgatggtct cagcttaccg gtctgtatca tcccaacaac aagttttcga acaaaatgtc
    15961 caagatgtga tgagtcgtca aggtgtatca gaagaggagg cgacaaagat tactaaagaa
    16021 acgattacgg agccaggata cagcgaacac cacacaggtc tggcagtcga tgtcgtcgat
    16081 caaaactggt acaacagtta tccttctact gtattggatg caagttacgg agatcagcct
    16141 ggagctaaat ggatcgcaga taatgctgcg cagtatggat ttatcgttcg ttatccaaaa
    16201 ggacacgaag atataactaa gatcacatat gaaccttggc acttacgtta tgtcggtaaa
    16261 gaaaacgctg agtatatcac caaacaccaa ttgacgctag aagaatatct gaatgaatta
    16321 aatgaaaagt aggaattgat catggcgaaa aaaaagtgga aaaagcaaaa gcgcaagaat
    16381 atccgttggc cagcagtggt tgccggtgta gcagtgatac tcttagcctt tgttttttcc
    16441 ttgaagaatc tgtcttctcc ttttactgat aatcagcaag aaaatgaagc gcaatcccat
    16501 cagcaattta ttgaacggct tgttccgcat gcccaagaac tacaagatgg ttatggagtg
    16561 cttcctagta tcattttagg acaagctata ttagaatcaa attggggaaa aagtaccttg
    16621 gctagcaaat acaataatct tttcgggatc aaggcttatg gggatcagaa gaaagtaagc
    16681 ttggaaacaa aggaatttgt taacgaagaa tggataacta tccaaggaga cttcaaagtg
    16741 tatgattcat gggaacagtc catggatgac catacacaac tgttcgtcca aggagtagat
    16801 tggaatcctg cgctgtatga aaaagtgatt acggccacca actatcagga agccgcacaa
    16861 gcattgcaag atgcaggtta tgcgacagac cccggttatg cgcaaaaaat catccaggtc
    16921 atcgagacgt atcaattgaa tcagtatgac cggagatagc aaaggagaag tggctgatgg
    16981 cagaacaatt tacgccagaa atcatgacgg aattgagaaa agatatcgta agagtaccgg
    17041 aaatcatttt agaagcaagc gggatcaaga tatttggaaa attgatccgt tcgatcatct
    17101 ttactacgga tattgcgatc atacgtaata caaatgcgaa tgcagtaatt gctgtgtatc
    17161 cttttacacc tcatccagcg attactaaaa gcattattga ggcagctgac attccagttt
    17221 tctccggtgt aggtggtggt ttgacacaag gtgctcgttc ttcctacatg agcatgttcg
    17281 cagaagcgca aggttcgtta ggggtcgttt tgaacggtcc tacgccttta gagacagtaa
    17341 aagaagtttg tcgagtagtt gatattccag ttatcagtac ggtaacgtcg aaatatacag
    17401 aaatagatga aaagttagca gctggtattt cgattatcaa tatcagtgca ggtaaagaaa
    17461 ctgcagaagc agtacgccat tttcgtaaaa aatatcctga acttcctatc attgcgacag
    17521 gtggtccaac ggaagaaagt attgctgaga ccattcaagc aggagcaaat gccatcacat
    17581 atacgccacc aagtagcgga gagcttttta gtcgtaaaat ggaaaaatat cgggaaactg
    17641 agaaaaagaa tcatgaataa caggtgagaa tcaaaagacg aggaagagca gatagtggct
    17701 cttcctcgtc ttttgtactc cagtactatt tgttcagaat tcaaaaaaaa gagaaatttt
    17761 tttgtttttt tacctgtaat gtacataaga agagttaaaa ccttttgtta ttattagcat
    17821 tctaacaaaa aaatgtcttg atttattgaa gcgctttcta tagaatgaaa aacgaataaa
    17881 aactaaaaaa ggcacccgac aatttagtag cagctaaatt gtcggggctt gaaaagtcag
    17941 cgggaactga ccaatcaagt tgccatagtc aatgcttgtg catcgacgtg tatattgtac
    18001 ctaatttcat acagaattac tagttatttt tctgtttttt agggaagagc gcacacgaag
    18061 aagcatgctt atttagctat gtcaaggata ttgggcagtt gtctgatatc cttttttctt
    18121 tgcgtaatta tcctcccatc attgaagcaa gaagtgtttt tagcgatgaa agaagaatca
    18181 gcactatcta atttttcttg aaagtattga gttgttcatc ctaggcagaa cgataaggca
    18241 agtcttggtt tttattcccc ttgaaaaaga tcgtttaata aatatgttct gtataaaaat
    18301 acagtatatt gaaaaataac aatggaagta gaggtgcaga gaatgaagaa aagaatgaga
    18361 atcatcttag ctttaagtgt tatgttattc ttcttgacgg cttgtgggca agaagatgac
    18421 aaaaaaatca cgctgaacat ggcttggtgg ggttcgcaag tccgacacga tgcaacagta
    18481 aaagtaatcg agctttatga agaaaaaaat ccccatgtga cgatcgatta tgaattttat
    18541 gattatgaag gatattacac aaaattaggt acactagcag catcagatga tatctgggac
    18601 attttccaac tgaacaatca atttcctcaa tacatcaatc agattgaacc attagatgaa
    18661 tatgtggagc aagggataat tgatatttct aatgcagatg aatcctattt agcaacgacg
    18721 acttacgacg ggcaattagt tggactgtct aacggggtga attcgtttgc gattgcttac
    18781 aataaaaata tatttgaaca attaaatatc cctgaaccag cttcaaattg gacatgggat
    18841 gaatttgaac aaatcagcta tcaaatcact gaagaattag gaatctatgc tatttcaaga
    18901 ttcgaagatt ttatagctgg ctgtattatt caaataccac aagttgagaa aggattgaat
    18961 ttttataatc gggaagatct atctaacagc ttgggatttg aaaatccaga ctatctaaca
    19021 gattttttcc agctgcgtaa aaatctggta gacaaagggg cccatccaga accaggtggt
    19081 ggagctgcaa gtacgacgga tctgtctttt caggcggtta gggatagaga agcggcaatg
    19141 gtgtttctat caagtaacca gttgacagaa atattgtcag gggctcctga agatatggag
    19201 attcagttga ttaatccacc tcgcagaaaa gcagatggag aatctggtat ccctttacgt
    19261 tcttcgcaaa tgctgagtat ggcaaaaagc tcagcccata aagaagaagc tgctaagttt
    19321 attgatttct ttcaaaacag tgaagaagcc aatgaaattt taaaaggaga acgtggtgta
    19381 ccaatcatgt cgaatattcg agaacagctc gctcgtgcag atgatgcaac tttgcaagcg
    19441 acatttgatt atttagaaat gatttccgat atggacaacg gcgaagtgaa tgtctttgat
    19501 agtgctttga atccggaaat tcaagaccaa tacaatcttt tagttgaaaa agtcatttat
    19561 gaagaagtaa cagcagaaga agcagcagag agtctttatg agtttgcgtc aaacattcta
    19621 ggatagcagc aggaggaagg catgaaaaaa gaaaaactaa aacggttctt ggatcaagac
    19681 aatgtggtcg gttacgtatt tgcttctcca ttcatcctag gatttctatt gataacagtg
    19741 ataccgatga tcttgtcact ttattattcg ttcaccgatt atcgtttggg aaatccagtc
    19801 tcatggatcg gcttggataa ttatataaag atgctgagtg atgcccgttt tttgaactcc
    19861 atccgagtca cttttctcta tgtatttgca tctgtacctg tgaaactgat ctttggtcta
    19921 ttgattgctt atcttttaac gaggaatatc aaaggaacga ctttctttcg ttctttgtat
    19981 tatgtgccta gtttgatcgg tggcagtatt tctatcgctc tagtttggcg ggagttgttt
    20041 tctaaaaacg ggttagtgaa tattttcctg gaacgattag gcgcacctgc gctttcctgg
    20101 tttagcgatc aaaaactggt catgatccct cttgttttga tgtctgcttg gcaattcggt
    20161 tcggcaatga tcatctttgc tgcaggtatc aaagagatcc caggctcgta ttatgaagcg
    20221 gcagagatcg atggagcgaa caaatggcag acgttttaca agatcacgtt acctcttctt
    20281 tcgccgatca ttttgtataa cttggttatg cagacaatcg cggcgtttat gacttttacg
    20341 caggcattta tcattaccca aggtggtccg aatgatgcca cgaactttta tgctctttat
    20401 gtgtataacc aagctttttc ttttaattct atggggtatg ctagtgctat ttcgtggatc
    20461 atgctgttga ttatgggagc tgtcaccttt ctgattttca aatcatcaaa aaaatgggtc
    20521 gttgtgtgaa tgagaggagg aagaaatgaa taatacagta acgaaatggt ttcaatacgg
    20581 ttttattttg ctattaggtc tagtcatgat ttatccggtc ttatggatga tcgcaggttc
    20641 ttttaaaacg gatcaagaga ttatgagtgg ttcgctgaat ttgattccag agacttttca
    20701 atgggaaaat tacgcaaaag gatgggcggg gtttgctgga atttcatttt ttactttctt
    20761 taggaattcg tttgtcatct ctgcagtttc gacgataggg acagttctga gctcgacttg
    20821 tattgcttac gcattatcga gaatcgattt caaaggaaaa agattctggt tcgtcgtcat
    20881 gttagtaacg atgatgattc ctgcacaagt gattttgatt ccgcagttta ttatttacaa
    20941 tcggctgaat ttggttggga cgtatgtgcc gctgatttta ccacattttt ttggacaagc
    21001 atttttcatt taccagatca tgcaattcat ggtcagcatc cccaaagagt tagatgaatc
    21061 agcgattatt gacggatgca gcaagtatag tgtctttacc aaaattattt ttcctttgct
    21121 gaaaccttcg atcatcacga cgatcatcat ccagttttat tggaaatggg acgacttcat
    21181 gggaccattg atttatttaa acaagccgag atcttacacg gtatcgattg ccatcaaatt
    21241 atttgcagac gctggatcta gtacgactta cgcgagtatg tttgcgatgt ctacgttgtc
    21301 gctgctaccg gtttttctga ttttcttatt cttcaataaa tatcttgtcc aggggatcag
    21361 tacaagcggt ctgaagggct gaatatgaaa aagccaatag aagagatgtt tctttacaag
    21421 ctcaactact atgtctgtcg gattttttta accagtctta tcagtaatgc tcttttcgct
    21481 ttagttctag gatgtttgtt cactttagga ttagtgctgt ctgctcgtgt tgtctatctg
    21541 atttattttt tgatgatgta tccttcttat tatctatctg tccattactt ggcagaaaaa
    21601 aaggtatgtt ggaggaaagg aaaaatcaaa cggatgtgtt tcatcattac gttgtttcag
    21661 ctgtttgtca ttatttttgc agttgattgg tatttattag tgggaaagat tggagcttgg
    21721 tatctgattc cttttctatt gtgtctgttg ctttttgtat cccatagttt ttttagtacg
    21781 ctaattgata tctgcaaaga agataaagca gatagtcttg tttatttgat caaaaggaat
    21841 atctggctgt tgtcaaaaaa cgtcaagggg tttctgtggt atttatgcct tttgtatgga
    21901 ctgttctggt gtatacagca tgatttttta ctcactgcat tactgtatcc agggctgcca
    21961 tttgtcacag tattttgctt aaaagaaaag gagaatgact ttaaatgaag aaaaaatatg
    22021 tacaagtcgg acttggtggg agagcgcgtt ttttttatga ggcaattgcc tctacgtatc
    22081 atcaaacttg tgaactagtc ggtttttgtg atgtatctca aacaaggatg gattatgcaa
    22141 atcggatact tgtgaacaag tatggacaga aaaaagtaaa gacatgtatg ccatcacaat
    22201 ttgagcaact actggatgac gtacggccgg ctgaagtcat tattacgtca gtagatcgaa
    22261 cgcatcatga ttatatcatt cgtgcaatga aaaaaggata tgatgttatt tgcgaaaaac
    22321 ctctgactat cgatgaggaa aaagctcaag aaataataac ggtccaaaaa cagacgggaa
    22381 gaagcctgcg agtcactttt aattatcgtt atgcaccgta tcacacgaag ataaaggaac
    22441 tgatagaaag tgatgtgatt ggtgatgtgt tttctatcca cttcgaatgg ctgttaaata
    22501 cgcaacacgg ggcagattat ttcagaagat ggcatcgaaa taagcacaat agcggcgggc
    22561 ttcttgttca taaatctacc catcattttg atctagtgaa tttttggttg aacagccgtc
    22621 cccaaaaggt atttgcattc ggtggattaa acttttatgg gatgcataat gcggagaaac
    22681 gcggagtaga aatattttac agtcgatgct atggaagcga actagctaaa gctgatccat
    22741 ttgctataga cctgtcagcc aatgaggaat tgcgagaaat gtatttagaa gcagaaaaag
    22801 acagtggata tattcgtgac cgcagtgtat tttccgatga tatctctatt gaagatacac
    22861 taggcgtaac ggtcaaatat gaaactggtg cgattttgac gtattcgcta aacgcgtata
    22921 tgccgtggga aggattcaat gtttctttca atggcagcaa aggacggatc gaaatgaaag
    22981 tgatcgaaaa gtcttacatc aatgcaggtg gagataaaaa ggatgaagga gcagccgttc
    23041 ttcagtccat ccatgtctat ccgatgttcg gtgagccata tgaggtaatc gtcgaacaag
    23101 aagcgggcgg acatggcgga ggagatccgc gtttattaga tgatctattt ggtgaagaaa
    23161 aagaagaaga tccgtggaat cgggcagcaa ctcacgtaga tggtattttg tcgattttga
    23221 caggaatcgc agcaaatcat tctattgctt caggaaaagc agtagtgatt gacgagcttg
    23281 tcagcttaaa agaataagaa tagatttacg gaaattactt cccgtagttt ataaaatctt
    23341 gccttattgc cgataggctg tcttttgaac actatttatt cggaaaaagg ggctatgaca
    23401 tgctttttgt catgccttct ttttatgtgg gctgcttttt tagataggag aaaataaaaa
    23461 taactattta aacccgaaca aaaaggaata atgcattaaa aatcattaga ttttacaaaa
    23521 aaataagtaa accacgttgt cagtttcaga aagtagtgtt agactgtttt caatatttga
    23581 aacaacattt tttattcaaa tatggcaaac tgccttcacg tgcaaaacag ttgcaagaaa
    23641 atgaatttac actattatta gttttacata cctacttata tatcgtcata atatggatga
    23701 cagtttctag ccagtaccgt aaatgctgga ctataagtaa aagattggct atttcaccct
    23761 tcttttctta tgataaagtg gagggtgttt ttctttaaca gaaacatagt aggtttttgc
    23821 acaaaagcgg ttatttcggt attaataaat gataaaggag caaataaagt gaaagaatta
    23881 gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa agtcgacagc
    23941 ttcgtcactc accaagtaga ccctgtattg atggaacaaa tgggaaaacg gtttgcagaa
    24001 gtatttaaag aacaaggaat cacgaaagtt gtgaccattg aagcttctgg gatcgcacca
    24061 gctttatttg ctgcaagaga attagatgtt ccaatgatct tcgctagaaa agcaaaaagt
    24121 ttgacgatgg atgaagaatt attgacagct tctgtttatt cttttacgaa acaagtgaca
    24181 agtacgattt ctatttctcg taaattttta tcttcagaag acaaagtatt gattatcgac
    24241 gatttcttgg caaacggaca agcggcaaaa ggattgatcg aattatgcca gcaagctgga
    24301 gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg acgtcagtta
    24361 cttgaagaaa tgggattgag agtcgtttct cttgcgcgta ttgcttcctt gacgaatgga
    24421 caagttgagt ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa aatggaaaag
    24481 ctgcggtttt agggctgcag catctgctag cgatgtatgc aggagcagta gcggtgcctc
    24541 ttttgatcgg tacaggacta ggctttaatc aagaacagat gacttatttg atttcaattg
    24601 atatctttat gtgtggatta gcgacattat tgcaattgac agttaaccga tttttcggaa
    24661 ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt agctccactg atcttgatcg
    24721 gaaccaatga aggagtagga gctatctacg gatctattat cgcttctggg atctttgttg
    24781 tgttagtttc tggtgtcttt tctaaaatta aaaaactatt ccctcctata gtaacaggaa
    24841 cggttatcac cgttattggt ttgactttga ttcctgtagc aatcgagaaa atgggtggag
    24901 gtaatgcaac agcagctgat tttggtgaca aaacaaatct gctacttgca tttgtgacga
    24961 ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt cattcgttca atcgcggtat
    25021 tgattggttt agtcggagga agcatcttag ctgctttcat gggaatgatt gatctgagcg
    25081 cgattggaga tgcgccgttg ttccatgttc cacaaccctt ttattttggt aaaccaactt
    25141 tcgacgtatg gtcgatttta ctaatgatca tcatttccat ggtcagcatg gtagaatcaa
    25201 caggtgtata ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa gaagaattga
    25261 aaaagggata tcgtgcagaa ggacttgccg ttatcttagg cggtattttc aatacattcc
    25321 cttatactgg cttttctcaa aatgtcggat tggttcaact ttcgggaatc aaaacaagaa
    25381 aacccatcta tttctctgca ttcttcttga ttgttttagg attattccct aaaatcggtg
    25441 cgttagcaca aatcatccct gagccggtac ttggtggagg aatgttagtc atgttcggga
    25501 tggtggcagt ccaaggaatc cgtatgctgt tggaagttga tttcagcaat gataaaaatc
    25561 tattgattgt tgctgtttct gttggatttg ggctgggttt caatgttacg ccgacattat
    25621 tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta atgagtagca
    25681 ttacggctat catattgaat ttgatcttta atggattgaa aaaagatgaa gaaatattgg
    25741 aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg ttttgacctg
    25801 attgttagag aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt ttgagctaat
    25861 accgagggtc agctgattga acaccgttga aggaagaggt cgtgagcctg ccgttcatct
    25921 ccgagtcaca aggaacaatt tcaaaaaaaa gcttctcatg tttgttgaat attgttcctt
    25981 gtgaccgagg agttggcagg agaacaccgt ttatccataa aaaggaatgt gacctaactt
    26041 ttgtcacatt cctttttttt atctttaata aacacgaaca ataaaatgtt tttctttgtt
    26101 aacattcgaa atttctttga catcaaaaaa agtgattgtt aaaatatgaa tgtagaaaaa
    26161 ctgggaggaa aaacaatgac agcaaaaatc tctgtgatta tggggagtac ttcagattgg
    26221 gaaacaatga aagaagcatg tcaaatattg gatgaattcg gtgtgtctta tgaaaaaaag
    26281 gtcgtttctg ctcatcgaac cccagatttg atgtttgaat atgcacaaaa cgctcgaaaa
    26341 aaaggaatca aaatcattat tgcaggagca gggggagccg cgcatctgcc gggtatggtc
    26401 gcatcaaaaa caactttgcc ggttatcggt gtaccggtcc aatcacgaac attaaatggt
    26461 ttggattcgc ttctttcgat tgtccaaatg cctggaggag tacctgtagc tactacagca
    26521 atcggaaaag cgggagcaat taatgctgga ctacttgcga tacagatgct ttccatgtat
    26581 gatttagaat tggaggctaa attggctgaa agacgttcaa tgttggctaa aactgttata
    26641 gaaagtagtg atcaacttgg ttaaaccttt gttgcctggt tcaacgatcg ggatcgtcgg
    26701 tggcggtcaa ttaggtcgta tgatggcgat tagtgcaaaa gaaatgggct ttaaagtagg
    26761 tgtgctggat cctgtgagcg attgtccagc cgcacaagtt gcagattggc acattgaaag
    26821 tgtctatgat gacacctttg cgctgaaaga gcttgccaga agaacagatg tcattaccta
    26881 tgaattcgaa aacgtcagta tagaggcgtt aaatgcgatt ttacccatgt catttattcc
    26941 tcaagggaca gacttgcttg cgattacgca agaccgcctg ttagaaaaat cttttttgga
    27001 aacgaataac atcgtgattg ctccttatgc aacgatcgtc agtccgacgg atatccaaga
    27061 tgcgattgac gggatcggct atccttgtgt tttgaaaacc actcgaggcg gctatgatgg
    27121 caaggggcag tatgttttga aaagtcgggc ggatctagct cctgccatgg accttttaag
    27181 agaaggaacc tgtgttttag aagcatggat cccgtttgaa aaagagattt cgatcatggt
    27241 ggcaggaaat ggtcaaggag atttcacgac ttttccggtc gtggaaaaca tccaccacaa
    27301 caatattctt catgaaacga ttgctccagc cgcgattgat caggatgtga tcgaggaggc
    27361 tgagcgtatt gcccgtgtga ttgcagaagc agtgagcctt tcaggtgttt taggggtaga
    27421 aatgttttta acaaaaacag gcgggctata tgtgaatgaa ttagctcctc gtcctcacaa
    27481 ttctggacat tattctattg aagcttgttc tatgagtcag ttcgatgccc atattcgagg
    27541 tatttgtggt tggccattag gagaaaaagt acgtttgata agcaatgcag taatggtcaa
    27601 tgttttggga gaacaactgc tggaaagcta tcgcttgatt gagaataagc cagactggca
    27661 attccatttc tatggaaaaa aacaagcaaa aaaaggtcgc aagatgggac atatcactat
    27721 cttgacagaa gacatccagc aaacgctgga agaaatcaaa caaacgaata tttgggatta
    27781 gaggagaaaa aacatgttag aacgttatac acgccctgaa atggggaata tctggacgga
    27841 agagaacaaa taccgtgcgt ggctcgaagt cgagatttta gcagatgaag cttgggcaga
    27901 acttggtgaa atcccgcaag aagatgtgaa aaaaattcgt gagcatgcat catttgatat
    27961 cgatcggatc ttggaaatcg aaaaattaac acgccatgat gtcgttgcgt ttacacgagc
    28021 ggtttctgaa acattgggag aagaaagaaa atgggtccat tatggtctaa caagtacgga
    28081 tgtcgtagat actgcttatg gctatctcat caagcaagca aatgatattt tacgggaaga
    28141 cttgaaacgt tttacggaaa tcattggcga aaaagcaaaa gaacacaaat atacagtgat
    28201 gatgggtcgg acacacggtg tccatgctga accaactact tttggattga aacttgccac
    28261 atggtattca gaaatgaaac ggaacatcga acgatttgaa catgcggcaa aaggagtaga
    28321 agcaggaaaa atctcaggag cagtgggaac gttcgcaaat atcccgccat ttattgaaga
    28381 gtatgtatgt aaacaattgg ggatccgtcc acaagaaatt tctactcaag ttctccctcg
    28441 ggatctacat gctgaatatt tctcagcgat ggcgcttatt gctacaagta ttgaacgttt
    28501 tgcaacagag atacgcggat tgcaaaaatc cgaaacacgt gaagtagaag aattttttgc
    28561 gaaaggtcaa aaaggttctt ctgctatgcc gcacaaaagg aatccgatcg gttcagaaaa
    28621 tatggctggt ctagccagag tggtgcgcgg acacatggtc actgcctatg aaaatgtcac
    28681 attatggcat gaaagagata tttctcattc ttcagcagaa cgaatcatct tgcctgatac
    28741 cacgatttta ttgaattaca tgttaaatcg ttttggcaat atcgttaaaa atctaacggt
    28801 ctttcctgaa aacatgaagc ggaatatgtc tgcaacattt ggtttgattt atagccagcg
    28861 cgttatgctg aaattgatcg ataagggaat gacaagagaa gaagcttatg atcttgttca
    28921 gccaaaaact gcgtacgctt gggacaacca agtgcagttc cgcccgctgt tagaagaaga
    28981 tccaaccatc acatcggtat tatctaaaga agaaatagac gatgcctttg attatcatca
    29041 tcatttgaaa aacgttgacg tgatattcaa acgagtaggt ttggaataaa gaagagtgag
    29101 ttgcaaaatt caattaaaat agaatagcaa aaataaccaa aaaagaagca gatccgttag
    29161 cggttctgct tcttttagtt tattatattt tcttatcaat aataggaacc tgattttaaa
    29221 agcggctacc tctttaccgt gcctctttac atacaaaaaa tagaggaatt aagcgtaaat
    29281 ggcttggcta ttataactaa aatgctggtt atggagatgt tgcagcaagt tttcgagaaa
    29341 agcgtattcc atctcttgtc cattgaataa gatctggata cgataatgat ttttgaaacg
    29401 cttctgttca tgaatctgac tgattgaaag ttcgcctttg tgttctgtct gataaagaaa
    29461 agtcctttta cggcattcag cacagcgctt ggttgctcgg cggatttttc ggaatgtata
    29521 aaaactagta tctttattgc ataaataaac gtctttgatc gacagcaaat cattatatag
    29581 acaggaacgt tcttcagctg ttaattctga taaaaaaagc tgaccttgtt ttattgcatc
    29641 ttccatcggc aagtgcctcc tcgaacccat tatccaaatt tttagttaat tctaatatac
    29701 catcattaag tataaatgta aacgcttgtt gatggtataa gattttcgtt tctgataaaa
    29761 cacgaacatt aaaagaatct aatgccttaa atgtacatat ttttgttgat ttataaaaag
    29821 atgattgcta aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg cttttaatgt
    29881 aaataaaaag gagagacgta catggagaag aaagaacttt tgtatgaagg aaaagccaaa
    29941 aaaatatatc ttacttctga tgagaacttg atttggatag aatatttaga tcaagcaact
    30001 gcgttgaatg gcgcgaggaa agaccagata agtggaaaag gagaattgaa taatcaaatc
    30061 acaagtgcaa ttttccgtta tctgactcaa agaggaataa aaaatcattt tattgaacaa
    30121 ttgtctaaaa ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga agtggttatc
    30181 cgaaatattg cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc
    30241 atcaaaccaa tcattgaatt ctactataag gatgaccagt tggatgatcc atttatcaat
    30301 gaagagcata tctatttctt acaacttgca aagccggaag aaatcagtga gctgaaagcc
    30361 caagcgttgg ctattaatga aattttgacc gaattatttg ctaaggtcgg tttggtttta
    30421 gtagatttca agctagaatt tggccggtcg aatggacaaa ttttgcttgc ggatgaagta
    30481 acaccggata cttgccgatt atgggatgaa cgtacgaatg aacatatgga caaagacgtt
    30541 taccgcagag atctaggtga tttgatccca gtctatcaag aagtatataa ccgtctatcg
    30601 gaactaactg aagaaaagga gcgagtatga tgtatttcgt aaaagtatat gtaacctata
    30661 aagattcagt gttagatcct caaggggagg cagtaaaaaa agcagtccat cgtatgggat
    30721 atgaaacaat cgaagatatc cgaataggaa aatactttga gatacaactt gctgatgcag
    30781 aagaaccaat cgatacagtg attgaaacga tttgtgacaa gctgcttgca aatgtaaata
    30841 tggaaacata ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc
    30901 atcgtttttc caggttcaaa ttgtgacgca gatatgttat gggcagttcg cgagatcatg
    30961 ggcgctgatg ccgaatttgt acggcatgat gagtcctctt tagaaggatt tgatggggtt
    31021 ttactgccgg gaggattctc ctatggtgac tatttgagat gcggtgcgat tgctcggttt
    31081 tctacaatca tggaagaagt gattcgtttt gctgaagaag ggaaaccagt attcggtaca
    31141 tgcaatggat tccagatatt gacagaagca ggacttttgc caggtgtact gagacgaaat
    31201 gagtctttgc attttatttg caaaactgta ccattgaaag tcaaccccaa gacggccttt
    31261 acttcagagt atgaagccga cgaaataatc tatcttccag ttgctcatgg ggaaggaaat
    31321 tatttctgtg acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat tgcctttact
    31381 tataatgaca acatcaacgg cagcgtggaa aacattgcag ggattacgaa caaagcaggg
    31441 aatgtgttag gaatgatgcc tcatccagaa cgtgcagtag aagacttgct aggatcagaa
    31501 gatgggaaac ggttctttgc ttccatttta aaaaacttcg gaaaggtgac cgtatagatg
    31561 aaaacattga aagaaccgac agctgaagag atcaagaaaa aaagaatcta tgccgaatgg
    31621 ggattgactg atgaagaata tcgaatgatc gaagaagata tcttaggaag aatgcctaat
    31681 tatacggaaa ctggactgtt ttctgttatg tggagcgaac attgttccta taaaaattct
    31741 aaaccggttc tacgcaaatt tcctactagt ggacctcatg tgttgcaagg ccctggagaa
    31801 ggcgcaggca tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc agaaagtcac
    31861 aatcatcctt cagccgtgga gccttatgaa ggagctgcga caggagttgg cggaatcatc
    31921 agagatattt tcagcatggg ggctcgtcca attgcgttgc tagattcatt gaggttcggc
    31981 gaattagata atccaagaac gaaatattta ctagaagaag tagttgcagg tatcggcgga
    32041 tacggcaatt gtatcggcat accgactgta ggcggcgaaa ttgcatttga tccttgttat
    32101 gaaggaaacc cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa acatatccag
    32161 aaagggcaag ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc
    32221 gacggtatcc acggagcaac ttttgcctca gaagaattta atcaagaaga agaacagcaa
    32281 cgttctgctg tacaagtagg cgatcctttt atggaaaagc tgctgcttga agcgtgtttg
    32341 gaattaatct tgcatcattc ggattctcta ataggtatac aggatatggg agcagcagga
    32401 cttgtttcat ctagtgcgga aatggcttca aaagctggct caggacttat attgacatta
    32461 gatgaagtcc cacaaagaga aactgggatg acaccttatg aaatgatgtt atcggaatca
    32521 caagaacgga tgttgatttg tgttaaatca ggagaggaag aaaaaatcca agaactattt
    32581 cagaagtatg atctagatgc agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg
    32641 ctatatcatc aaggagaaga agtagcaaat ctgccagtcg atgcattagc tgaagatgca
    32701 ccagtttatc ataaagaaat gaaagaacca gctcgaatcg tagaatttca gcagttagct
    32761 ccttatcagc cagttatcga agaacctgga caagtattgc ttgatctgct gcagcaaccg
    32821 acgattgctt caaaacgttc aatctacgaa acttatgatt cacaagtaca aaccaataca
    32881 gttgtccgtc caggaagcga tgctgctgtg atgagggtta gagggacgaa taaagcgtta
    32941 gctatgacca cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa
    33001 atagcagtgg cagaagctgc tagaaatatt attgctagcg gtggtaagcc cttggcgatc
    33061 accgactgcc tgaactatgg ttctcctgat aaaccagaag ttttttggga attatcgact
    33121 tcagctgacg gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat
    33181 gtctctttat ataatgaaac ggatggtcaa gcaatctatc caacaccaat gattggtatg
    33241 gtcggattga ttgaggatca taaacatatc actactcagg aattcaaaaa atcaggggat
    33301 ctaatctata tattaggtaa gacgtttgca gattttgatg gcagtgaatt acaaaagatg
    33361 cagcttggaa gaatcgaagg tgtgatccga aactttgatc tatccattga aaagagaaac
    33421 caagagttag tattgacagc catccaaaac ggattgattg aaagtgctca tgactgttca
    33481 gaaggtggct tggcaattgc actggcagaa tcagctttca aacatcagct agggatatct
    33541 gtacagtttg aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg
    33601 acagtcgcac cggaaaataa gacacgattc gaagaaatga tgggagatgc tgctgtttta
    33661 gcaggtaagg tgacggatga agcgataatc gaaatttcag caactgacgg tcagatcaaa
    33721 atagaaactg cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga
    33781 agagtttaaa tgaagaatgc gggatcttcg gtgtttgggg tcatccagac gccgctagtg
    33841 tggcatactt cgggctccat agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt
    33901 ccaatgatcg aggaaagctt aaggggcatc gagatttggg gctgctttct gaggtattca
    33961 aagatcaaag aaaattgcag cagttaacag gagaagctgc aatcggacat gtacgttatg
    34021 caacagcagg caatggaagt gtggacaata ttcagccttt tctattcaaa tttcatgatc
    34081 agtcgatcgg cttagcgcat aatggtaact tgatcaatgc tcgttccctt agaaaagagt
    34141 tagaggaaaa tggggcgatc ttccattcta actccgatac ggagatattg atgcatttga
    34201 tccgacgaag tgaagaaaca gattttttaa ataaattaaa aagtgctttg aatgaagtca
    34261 aaggtggctt tgcctattta ttaatgacag aaacggcaat gattgccgct ttggacccaa
    34321 atggttttcg tccattagca atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg
    34381 aaacttgtgc attagaagtt attggggcac gttttataag agatgtccag cctggtgaga
    34441 tagtgatcat caatgacgaa ggaatccaga ttgattgctt tacgaaggac acgcagcttt
    34501 ctatttgttc gatggaatat atctattttg ccagaccaga ttcaaacatc gcagggatca
    34561 atgtgcatac tgcacggaaa aatatgggac ggaatttagc gaaagaatcg ccagtaaaag
    34621 cagatatggt cgttggcgta cctaattctt ctttgtcagc cgcaagcggt tatgcggaag
    34681 ctagtggaat cccttatgaa atcggattag taaaaaatca atacattgcc cgaacattta
    34741 tccagccaac gcctgagcta agagagcaag gtgttcgaat gaaattatct gctgtacgag
    34801 gagtagtgga aggtaaaaag gtgattatgg tcgatgattc aattgttcgg ggtacgacaa
    34861 gcagacggat cgtccatctt ttgaaagaag caggggcaaa agaagtccat gtgaggattg
    34921 cttctcctcc tttgaaatat ccatgttttt acgggattga tattcaaacg cgtaaagaac
    34981 tcattgcggc aaatcacacg attgatgaaa tcaaagaatg tatcggtgca gattctttag
    35041 gttttttaag tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt
    35101 caggattgtg tatggcttat ttcaacggag attatccgac gccgttatat gactatgaag
    35161 aaaaatatca ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag accaagaagg
    35221 gagtatatga attcaatggc aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga
    35281 tatgaagtag tagaacggat caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg
    35341 ggcgctatag gaggattcgg cggttgtttt gatttatcgg tctttcaatt aaaagagcca
    35401 gtccttgtct cggggaccga tggcgtgggg acgaaattag cagtggcaat cgaagcagac
    35461 aaacacgata caatcgggat cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa
    35521 ggcgcagagc cgttatattt cttagactac ttagcaacgg ggaaaaatga tccgttacgt
    35581 ttggaaaatg tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt
    35641 ggcggagaaa cagctgaaat gcctggtatg tactcaggta atgattatga tctagccgga
    35701 tttgctgtag ggatagctga aaaatctcaa ttattgacag gagaaacgat caacccagga
    35761 gatgtgttgc ttggaatagc atctagcggg attcattcaa atgggtactc tcttgttcgc
    35821 aaaattttct ttgagcagca gcagttttct ttatcatcaa aactgccaga attgaaaaaa
    35881 acacttggcg aagaattgct tgagccaacg aaaatctatg tttcttcctt gcttccgctg
    35941 atcaaagaaa agaaaataaa aggagctgca catatcactg gtggagggtt tgtagaaaat
    36001 atcccgcgaa tgttaccaga atctatggct gcacaaatca aacttggctc atggccgatt
    36061 cttccaatat tcggctgttt acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa
    36121 atcttcaata tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata
    36181 gtacacgaaa ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa
    36241 gaaaaacatc gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt tttgcttctg
    36301 gaaacggaag caactttcaa gcgctagccg attatctatc gaaaaaagga ttggaatctt
    36361 cgattgactg gttgttttgt gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt
    36421 tgagtgtacc ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag
    36481 aagcaatttt gcataaattg aaagaaaaaa agatcgattt gatcgtatta gctggatata
    36541 tgcgaatcat tggaccggta ttgctgaaaa attatgataa aagaatcatc aatatccatc
    36601 cgtctctttt acctgccttc cctggtttgc atggcatacg tgatgccttt gaagctggag
    36661 taaaagaaac gggtgtcaca attcattata tcgaccaagg tgtagatact ggaccaatca
    36721 tccggcagga aaaagtgaga attgaacagg aggacacgtt cgattcttta gaagaaaaaa
    36781 tccatagagt agagcatcga atctatccag aagtgattag tgaaattatt gaaaatggag
    36841 gctatctgaa atgacaagag cattgatcag tgtatcagat aaaactggga ttgtagcatt
    36901 cgcccaaggt ttgattgaac aaggagtaga aatcatttca acaggaggaa caaagcatgc
    36961 gttggataaa gctgggatca aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat
    37021 gatggatggc cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt taggaagacg
    37081 cgatctccct agccatatgg aggcaatgga aacggaaaat atccaaccaa tcgactttgt
    37141 ttgtgtcaat ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga
    37201 agctattgaa aacattgata tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca
    37261 tgaatttgtc acctctgttg ttgatccatc tgactatgaa gaagtacttt cagaattacg
    37321 tcagtatggg catacttcac tttctttcag acaaaaacta gcggcaaaag tctttcgtca
    37381 tactgcggca tatgatgcac tgatcgctca atacttaacg gattgggtgg aagaaaaaga
    37441 accggaaaaa ttgacactta cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag
    37501 ccatcaagcc gctgcctttt atcagtccgc tcttctggaa aaatattcga tcgcagcagc
    37561 aaaacaactc catgggaaag aactttctta taataatatc cgagatgcag atgctgcgct
    37621 acggatcatg aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg
    37681 tggtattggg acagcatcta cgattttaga agcgtgggaa gcagcgtatg aagcagatcc
    37741 tgtttcaatc tttggtggga tcatcgtttt gaatcgagaa gtagatctgc caacagcaga
    37801 acagatgcat aagctgtttc tagaaatcat cattgcccct tcttatacac aagaagcttt
    37861 ggaattactg caaaaaaaga aaaacatccg attactgaca gtagactttg cgcaggaaga
    37921 aaatcaaaat cgagaagaaa cggtctcagt cttaggtgga cttcttgtac aagatcaaga
    37981 tctggcaata gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc cgactccagc
    38041 agaagcaaaa gcattagtgt ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat
    38101 cgtactggca aacgaacgtc agacagtagg aatcggagcc gggcagatga atcgagtcgg
    38161 ttcagtaaaa attgcgattg aacaagcgca agagaaaatg gaaggtgcag ttttagctag
    38221 cgatgcttat tttccaatgg atgattcggt tgaatatgca gccaaacatg gtataaaagc
    38281 aatcattcag ccaggaggaa gcatcaaaga ccaagattct attgatatgg cgaataaata
    38341 cgatctagca atggtcttta ctgatgttcg gcattttaga cattagagag gagtcggata
    38401 aatgaacatt ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt
    38461 agccagtccg aaggtggata cggtgtattg tgcgccagga aatccgggga tgacaaaaga
    38521 tggtatcaat tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc attttgttaa
    38581 gaaagaggct attgattgga gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt
    38641 cgatgatttt atggcggaag gattgaaaat cttcggtccg acaaaagcag cagctctgat
    38701 cgaaggttcg aaaagttttg ctaaacaatt aatggatact tatcaaatcc cgacagctaa
    38761 atccagaagt tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc
    38821 aatcgtagtg aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt
    38881 gaaggaagcg ctggatgcgg cagaacaaat gttgaaccag catcgatttg gggatagtag
    38941 caaaaaaatc gtcgtagaag agtttttggc aggagaagaa ttctcccttt tggcgtttgt
    39001 ccgtaatacc aaggtgtatc cgatggtcat ttcgcaagat cataagcggg catttgataa
    39061 cgataaaggg ccaaatacag gcggaatggg tgcttattca ccagtaccgc aaatttcaaa
    39121 aaagatgatt gaaaaagctg tggaagaagt tttaaaacca gcggctttgg gaatgaaaga
    39181 actaggacgc tctttcacgg gtgttttata tgcgggtttg attgctacga aggaaggacc
    39241 aaaagtgatc gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg
    39301 gttgaagagt gactttgcgc aagtaatcga tgatttatta gagaatcgta cagtagagtt
    39361 aaagtggcag caagaaggat acaatgtagg cgttgtggtt gcagctgccg gttaccccga
    39421 agaatatgag acaggaatgg ttctaccaga ttttctggaa gaagagcttt ctgtttacta
    39481 tgctggagtt tcctcaaata acggcaaact agttggagca ggaggacgtc tttatttagt
    39541 agaagcatac ggtgagacga tcaaagaagc acagaaaaaa atttacaccg cgttaatgga
    39601 taaaacgaca gatggaacat tttatcgtac agatattgga gaaaaagctg ccaatagcga
    39661 actgatcgtg aagtaaatca gctgcttcca tttataaaaa atacaacaaa cataatcgga
    39721 agcgccgatt ttagtgaagc cttttcataa agagtgtggt acacttttgt tgtgtatata
    39781 aataaggagg agttcagatg aaaaaaattc aattaggtac ttctgatcag aaagcagctg
    39841 cagtgatctt aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga
    39901 cagcttatga aaacggcatt gatttttttg accatgcaga tatttatgga aacggtgaat
    39961 gtgaagaaat ctttgcagat gctctagcaa aaacatcaat caaacgagaa gatcttttta
    40021 ttcaaacaaa atgtggaatc gttccaggtg tgatgtttga tttttctaaa aaacatatca
    40081 ttgaatctgt agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat gctttacttc
    40141 ttcatcgtcc agatacattg gtagaaccag aggaagtagc tgaagcgttt gatgaattgg
    40201 aacggtcagg aaaagttcgt tattttggcg tcagcaatca aaaaccaatg caaatcgaat
    40261 tgctgaaaaa atatgttcgt caaccattag tagctaatca gctgcaattt ggcttgaaac
    40321 atactggaat gatcgatcaa gggattcatg tcaatatgac agatgaagca agtatcgatc
    40381 atgacggtag tgtacttgat tattcccgat tgcatgatat gacgatccaa gcatggtcac
    40441 cataccagta tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa
    40501 accaaacatt agaagaacta gcagctaaat atcaaacgac accaactggc ttggcttctg
    40561 cttggatttt acgccatcca gccaatatgc aagtgatcgc cggtacgatg aacgtagggc
    40621 gaatcaagga aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat
    40681 tgtaccgtgc tgctggaaac attttacctt aaagattgtc ataagcaggt caaaagggct
    40741 ataaattaaa aagacagaat aacttgaaag ctgttttcag cttcgagata ttctgtcttt
    40801 ttttattgca aatggaaaga gtatcattgt acggatttcg tttattagtt atttttgttt
    40861 ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc cttgtttttt cttaaataaa
    40921 taactgaaat agttagggtc attataacca atttcaaaag caatatctga aatacgatag
    40981 tctgattgct gcaataattt ttttgctaat tcgattcttt gttctgtcag ataatcgatg
    41041 aatgtttggt taaacgattg agaaaaaatc gtactgaaat gagagggact taatgcaact
    41101 tgttgagcga cccaagttaa ggacatatct ggatcggtaa aattttcttt gataaaatcc
    41161 aatgcatggt tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg cagagcagtt
    41221 aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct
    41281 aagcgttgac tggtggatgt gcaatgaacc atttcttgcg ggattttctt ttctttcagg
    41341 cagacatgct ggagtagctt agtcaagaca aataaacgat ataatcgtgt tcgagttggt
    41401 gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata cttcgttttt cgataatgaa
    41461 gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt cgcttcttga
    41521 atcaaatcct catagctgat aatttgttct ttaagggaac tggcggtttc aagtatcatc
    41581 tgcgtgatct caaaagatgc aggtaattcg ctgattcttt ctgcgacagg accaatggcg
    41641 acagaaaaat tatttttttg tcccttttct aattcatgga ccaacgtttg ggctacctga
    41701 taactggtct cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga
    41761 gaggaaacag cagagaagat cacttgttga ttctccctaa ataaaaactt caaataatca
    41821 ctgagatgag cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa taatacggtc
    41881 atttttttcc ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc
    41941 gtaagtgagc ctttgaacaa accgttcaag aaatgatttt ttttcaattc gaaaacaaga
    42001 tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag cagttactgc tttttctatt
    42061 gcttgtttca attcgctact tttaatcggt ttaagcagat aatcatctgc ctgtacctgg
    42121 attgctcctt tcatataatc gaaatcgtcg aatccactaa taaatatgag acgagtccaa
    42181 ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg catttggata
    42241 tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag acagtgccat ctcaccatct
    42301 ccagcttcgc cgacataagt gattggataa taagaaccaa ggacagttat ttgttcccgc
    42361 aagttattgc gaatgaggta ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc
    42421 tccaattgtt ttgggagata gagggatcat cagtgtgaca gttgttcctt ctcggtattt
    42481 gctgtcaatc gtgataggtt tttgatcatt tccgtaataa agaaggagac gcttgtttac
    42541 gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc cctttggcta actctttacg
    42601 tatttctgcc agtttttctg cagggatgcc aatcccgttg tctataactt tgaattggat
    42661 catctcatca attttgcatg tggtaatacg aataaatcct ttgtgacgga cgtacttcgt
    42721 tccatggtat acagcatttt caatcaatgg ctgcaagatc atttttaaaa tagaaacagg
    42781 aaaaagtgtt tctggtatat cgatcttata gtcaagcata tggccgtatc ttatttttaa
    42841 gatgactaga taatcattga tatggctaat ttctttttca agaggaatcc agtcttgtcc
    42901 tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca gagcttcttc
    42961 aaagcagtct tgttcgatca aagaaataat ggcatccaaa ctgttataaa taaaatgggg
    43021 agtaatctga gcttgcaata cacgaacttc actttgtgct aaatggtatt gtttcaaagc
    43081 gttctcccgt aaagactggt ttagatcttc cgccatttta ttcatgctag cggtaagtgt
    43141 ttgtaattcc agtgtttccg gcagggttaa acgaaagcca aggttccctg aagaaagttg
    43201 ttctgacatc tcgatcagct gattgatcgg ttcttggacc tttctctcta aaaagcgacg
    43261 attctttttc gcatagaaaa aaatggtact aagaactcca agttccacta ttgttaatag
    43321 aatgagcgag cggacaagtg tcgtattttt tttgcttgct aagtcgatct ccacacgaac
    43381 aaaatcttgt aagatatctg acagcagttg agtgacagaa tctacctgat tgatgatctc
    43441 ttcaatggta tccataaaat cttttgccaa gatattattg atcagctcgt tttgataatc
    43501 ttccagtgta tcgatcgtac gtagcgatac atctaacgta ctaatttctt taagtgtatt
    43561 tgtattattt ttgatctctc gaatatcatt cctaagttct tctaggatat tttcctcatt
    43621 gaactcatct acggttactt ggccagaaat caagtcccac atactttcca aaactttttg
    43681 atcaaccttc aaagcaattg catctgcttt ttggatattt gcgatcgtct gttgataaat
    43741 caaaagctgt tgcgaatata acagtatgct cagtagcaaa gggataacag caaatagaag
    43801 catggctttg tttgcttggg caaaaatctg ttttatgctt aaattttttg tcatgatact
    43861 tctccagtca ataatttctg gttgggatgt tagctatatt ctgctgggaa aagacagtct
    43921 cggaaataaa aatatcttta ggtaatgttt ttcctgtttg aatatttcca aaataacgag
    43981 taacggtatt ggctacatac cagccgacat caggtttaca ctcgactaca caattgacga
    44041 ttcctgagcg tagcttctcg atcatatctt cttgtccatc aatagttaca atgatcaaat
    44101 cttctccagg tttgattgat gtttctttta tcgcagctaa agcaccgtga gtcatttcat
    44161 cactatggct gtataataca tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca
    44221 tctggtcttt tcctttcatc cgaataaaat caccgacaat tcggtgaaca atagtcatcg
    44281 gaggtttatg gtcgatggtt ccatcttttt ttagggttgg tttacgattg attgattcca
    44341 aaaatccttc ggagcgtagc ttggtcggtg tggagttctc tgaaccagca agttctagga
    44401 ttctgatttc ctgtttcttt gaatttgtga agtgattgct gacaaataaa cccgcccgat
    44461 ttccttctgc tttgaaactg gggccaatgt gggtgacgaa aagctcggga tcattcgttt
    44521 gaatatgtct gtcgacaagt atgacaggga tccctgctcg ctttgcttct tttaatacag
    44581 agtcccaccc ggattcttgc aaaggagtaa aaatgatcat atccacttta taggcaatga
    44641 acgtccgtat atcttggatt tgccgttcct gattcatgta gccatttcgg tagagaactt
    44701 ggtaatcttc tttttcaagc gcagtgataa ttgattcagt atgctttttc cgccagctac
    44761 tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc tttgtattag
    44821 gtgtacaccc catcatttgg taagcgatta caacgagggc taaaaaaaat aaaacacgtt
    44881 tcactttaaa tcactccatc tggatttgtt agtactatct tatcagataa aagggaaaat
    44941 caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa
    45001 aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta acgctttctt
    45061 tattagctat actcggtgta tcaattgaaa tgggggataa aaaatgaaaa acaaatttag
    45121 aaagattgtt gggactatcg ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa
    45181 ttcaggatct gcagatgata gtacggggta tgtaggaatc gctatgccga ccaaatcagc
    45241 agaaagatgg atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac tgggttataa
    45301 aacagatctt caatatggag aagataaagt agaaaatcaa gtagcacaaa tagaaaatat
    45361 gattacaaaa ggggtagata cacttgtgat tgcttctatc gatggttcgg ctttaactga
    45421 tgtgttagct aaagcaaaag aagcagatat caaggtgatc gcctatgatc gtttactgat
    45481 gaattcagaa aatgtcgatt attacgcgac ttttgataat tttggagtag gtgtatcgca
    45541 agcagcttat attgaagagc acttaggatt gaaagaagga aaaggaccat tcacgattga
    45601 attatttggt ggctcgcctg acgacaacaa cgcactaatc aattacaacg gagtgatgtc
    45661 tgttttacaa ccatatatgg acaacggtca gttggtcgtg ccatctggac agacgagttt
    45721 cagtcaaatc gctacgcttc gctgggatgg ttctacggcg caagcaagga tggataatct
    45781 gttaagcgca aattatactg accaaacatt ggatgctgtt ttgtcgccat atgatccaat
    45841 aagtttagga atcatttcgt cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt
    45901 acctgtgatt actggacaag atgcgacagt tgctggcgtt aaatcgatca ttgccgggga
    45961 acaaacacaa acgatcttta aagacacacg tatcttagca aaaaatacga ttgaaatgat
    46021 caaagcaatc agtgatggag aagaggttcc ggtcaatgat actgaaactt atgataatgg
    46081 ggtgaaaact gtccctacgt atttagcaaa tacagtatct gtcgataaag ataattatca
    46141 agcagagctg atcgacaccg attattataa agaatccgat ctgaaaaatt aagaggaacc
    46201 aaacaaggaa ctaaagagat ctaagtcaga gagaggataa gaattatggc ggattgtatt
    46261 ttaaaaatgg cagatatcac caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat
    46321 ctgatgattg aacgtgggga aatccatgca ttgtgcggag aaaacggagc gggaaaatca
    46381 acattgatga atgtcttgag cggcttgtat ccatatggct cctatgacgg ggaaatcttt
    46441 tatgatggaa agctgtgtca atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc
    46501 atcattcatc aagaattagc actaagtcct tatctttcaa taaaagagaa tatttttctc
    46561 ggaaatgaac aagcagaaag aggcgtgatt gattgggaac tgacagaaaa gaaaacatat
    46621 gaactgctga ataaagtcgg tttgtctatc gacccgaatc ttctagtatc ccagattggc
    46681 gtagggcaac agcaactagt tgaaatagct aaagcattat caaaatccgt ccgtctgttg
    46741 attttggatg agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt gctgagattg
    46801 atccgagagt tgaaagagca aggtgtaacc tccatcatta tttcacataa gctgaatgaa
    46861 atcgtggata tcgcagatcg aatcaccatt ttacgggatg gacagacgat tgagacacta
    46921 gaaaatgaaa tgatcagcga agagcatatt attcgcggaa tggtgggcag agatttagtt
    46981 gatcgttatc ctgaaagaaa accggatatt ggagaagttt atttt
//
LOCUS       DAFBYI010000007        46761 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFBYI010000007 DAFBYI010000000
VERSION     DAFBYI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.007"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..518)
                     /locus_tag="LLD04_000328"
     CDS             complement(<1..518)
                     /locus_tag="LLD04_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5647288.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIF"
     gene            complement(594..740)
                     /locus_tag="LLD04_000329"
     CDS             complement(594..740)
                     /locus_tag="LLD04_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK5647289.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(844..1155)
                     /locus_tag="LLD04_000330"
     CDS             complement(844..1155)
                     /locus_tag="LLD04_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5647290.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1157..1354)
                     /locus_tag="LLD04_000331"
     CDS             complement(1157..1354)
                     /locus_tag="LLD04_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK5647291.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1748..3475)
                     /locus_tag="LLD04_000332"
     CDS             complement(1748..3475)
                     /locus_tag="LLD04_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK5647292.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3468..4661)
                     /locus_tag="LLD04_000333"
     CDS             complement(3468..4661)
                     /locus_tag="LLD04_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5647293.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4848..5492)
                     /locus_tag="LLD04_000334"
     CDS             complement(4848..5492)
                     /locus_tag="LLD04_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5647294.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5586..5720)
                     /locus_tag="LLD04_000335"
     CDS             complement(5586..5720)
                     /locus_tag="LLD04_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK5647295.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5722..7854)
                     /gene="feoB"
                     /locus_tag="LLD04_000336"
     CDS             complement(5722..7854)
                     /gene="feoB"
                     /locus_tag="LLD04_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK5647296.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7851..8324)
                     /locus_tag="LLD04_000337"
     CDS             complement(7851..8324)
                     /locus_tag="LLD04_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK5647297.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8725..8949
                     /gene="nrdH"
                     /locus_tag="LLD04_000338"
     CDS             8725..8949
                     /gene="nrdH"
                     /locus_tag="LLD04_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK5647298.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9108..11267
                     /gene="nrdE"
                     /locus_tag="LLD04_000339"
     CDS             9108..11267
                     /gene="nrdE"
                     /locus_tag="LLD04_000339"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK5647299.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11317..12282
                     /gene="nrdF"
                     /locus_tag="LLD04_000340"
     CDS             11317..12282
                     /gene="nrdF"
                     /locus_tag="LLD04_000340"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK5647300.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12371..13510)
                     /locus_tag="LLD04_000341"
     CDS             complement(12371..13510)
                     /locus_tag="LLD04_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5647301.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13819..14532)
                     /locus_tag="LLD04_000342"
     CDS             complement(13819..14532)
                     /locus_tag="LLD04_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK5647302.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14786..15487)
                     /gene="nagB"
                     /locus_tag="LLD04_000343"
     CDS             complement(14786..15487)
                     /gene="nagB"
                     /locus_tag="LLD04_000343"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK5647303.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15721..17253
                     /locus_tag="LLD04_000344"
     CDS             15721..17253
                     /locus_tag="LLD04_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5647304.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17444..18646)
                     /locus_tag="LLD04_000345"
     CDS             complement(17444..18646)
                     /locus_tag="LLD04_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5647305.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18728..19417)
                     /locus_tag="LLD04_000346"
     CDS             complement(18728..19417)
                     /locus_tag="LLD04_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5647306.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19523..19891)
                     /gene="rplL"
                     /locus_tag="LLD04_000347"
     CDS             complement(19523..19891)
                     /gene="rplL"
                     /locus_tag="LLD04_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK5647307.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19944..20447)
                     /locus_tag="LLD04_000348"
     CDS             complement(19944..20447)
                     /locus_tag="LLD04_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK5647308.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20651..20778)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20857..21546)
                     /gene="rplA"
                     /locus_tag="LLD04_000349"
     CDS             complement(20857..21546)
                     /gene="rplA"
                     /locus_tag="LLD04_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK5647309.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21650..22072)
                     /gene="rplK"
                     /locus_tag="LLD04_000350"
     CDS             complement(21650..22072)
                     /gene="rplK"
                     /locus_tag="LLD04_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK5647310.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22415..23083
                     /gene="sdaAB"
                     /locus_tag="LLD04_000351"
     CDS             22415..23083
                     /gene="sdaAB"
                     /locus_tag="LLD04_000351"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK5647311.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23096..23968
                     /gene="sdaAA"
                     /locus_tag="LLD04_000352"
     CDS             23096..23968
                     /gene="sdaAA"
                     /locus_tag="LLD04_000352"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK5647312.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24059..25078)
                     /locus_tag="LLD04_000353"
     CDS             complement(24059..25078)
                     /locus_tag="LLD04_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5647313.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(25251..26156)
                     /locus_tag="LLD04_000354"
     CDS             complement(25251..26156)
                     /locus_tag="LLD04_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5647314.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(26215..26760)
                     /gene="nusG"
                     /locus_tag="LLD04_000355"
     CDS             complement(26215..26760)
                     /gene="nusG"
                     /locus_tag="LLD04_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK5647315.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26861..27031)
                     /gene="secE"
                     /locus_tag="LLD04_000356"
     CDS             complement(26861..27031)
                     /gene="secE"
                     /locus_tag="LLD04_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK5647316.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27055..27207)
                     /gene="rpmG"
                     /locus_tag="LLD04_000357"
     CDS             complement(27055..27207)
                     /gene="rpmG"
                     /locus_tag="LLD04_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5647317.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27369..28013
                     /locus_tag="LLD04_000358"
     CDS             27369..28013
                     /locus_tag="LLD04_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5647318.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28069..28572)
                     /locus_tag="LLD04_000359"
     CDS             complement(28069..28572)
                     /locus_tag="LLD04_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5647319.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28585..28631)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29169..30134
                     /locus_tag="LLD04_000360"
     CDS             29169..30134
                     /locus_tag="LLD04_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5647320.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(30233..31405)
                     /locus_tag="LLD04_000361"
     CDS             complement(30233..31405)
                     /locus_tag="LLD04_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647321.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31392..32537)
                     /locus_tag="LLD04_000362"
     CDS             complement(31392..32537)
                     /locus_tag="LLD04_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647322.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(32551..33475)
                     /locus_tag="LLD04_000363"
                     /pseudo
     CDS             complement(32551..33475)
                     /locus_tag="LLD04_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            33592..34671
                     /locus_tag="LLD04_000364"
     CDS             33592..34671
                     /locus_tag="LLD04_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5647323.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            34703..35296
                     /locus_tag="LLD04_000365"
     CDS             34703..35296
                     /locus_tag="LLD04_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5647324.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(35406..35633)
                     /locus_tag="LLD04_000366"
     CDS             complement(35406..35633)
                     /locus_tag="LLD04_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5647325.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(35736..38324)
                     /locus_tag="LLD04_000367"
     CDS             complement(35736..38324)
                     /locus_tag="LLD04_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK5647326.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(38344..39003)
                     /locus_tag="LLD04_000368"
     CDS             complement(38344..39003)
                     /locus_tag="LLD04_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5647327.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39052..39555)
                     /gene="trmL"
                     /locus_tag="LLD04_000369"
     CDS             complement(39052..39555)
                     /gene="trmL"
                     /locus_tag="LLD04_000369"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK5647328.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(39776..40642)
                     /locus_tag="LLD04_000370"
     CDS             complement(39776..40642)
                     /locus_tag="LLD04_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5647329.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            40751..41392
                     /locus_tag="LLD04_000371"
     CDS             40751..41392
                     /locus_tag="LLD04_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK5647330.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(41454..42815)
                     /gene="mgtE"
                     /locus_tag="LLD04_000372"
     CDS             complement(41454..42815)
                     /gene="mgtE"
                     /locus_tag="LLD04_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK5647331.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(42833..43732)
                     /locus_tag="LLD04_000373"
     CDS             complement(42833..43732)
                     /locus_tag="LLD04_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5647332.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(43734..44531)
                     /locus_tag="LLD04_000374"
     CDS             complement(43734..44531)
                     /locus_tag="LLD04_000374"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK5647333.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(44509..45213)
                     /locus_tag="LLD04_000375"
     CDS             complement(44509..45213)
                     /locus_tag="LLD04_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK5647334.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            45344..45919
                     /locus_tag="LLD04_000376"
     CDS             45344..45919
                     /locus_tag="LLD04_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK5647335.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46037..46714
                     /locus_tag="LLD04_000377"
     CDS             46037..46714
                     /locus_tag="LLD04_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5647336.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT        14118 a         9751 c         8225 g        14667 t
ORIGIN      
        1 aaaaatatta agacaccaaa cacaaaatga tcaaatactt gatattgatg agcaacataa
       61 gaaacaaata aaccaattgt aagtaaataa atcatgaata tagcagtaag tttagaactg
      121 gactgttcaa caatcctatt tatagctaat tttctatata accaaataaa catcagcaaa
      181 aacaaagaat caagcattat ctggaatact acataaccat acccttttat cccttcaaat
      241 gaaaacatat acgtcataac catggaagta ataatagatc tgaaatcatc aataagcata
      301 cataatagta atttatttaa aagcactata ttagttttct tcttttcctt taatataaaa
      361 taacttccga ttataggtat agctgcggaa acatcagtaa ataattcaac aaaaaaagtg
      421 agtggtatca ataaacatac aaaaagatat tttcttttgt taagatcagt attagtcata
      481 taaaataaga gaaaaaagaa aaagtttccc acgaacattc ttaatagata agcttttgta
      541 tcgatagtaa tcaagagatc ccctcctgat tttaggatat cataaaaaaa attttagtta
      601 cactttttaa ataatgaaaa aacacattcc actaaatttg atgccggttt tccttgaggt
      661 tttgttccag cacctccttt aattttttta agctcttgat ctgaacattg tttagcattt
      721 agtctatttt tttcttccat tacgattccc cctacaaact ataaatactt ttatgtcact
      781 gattcaatat acctattttt ctaatcaaaa aattttgaga catatttaat attataagta
      841 tcattaaaat tgagatttat ctccataatc tgctcgatat aaaccattta ttccagcagt
      901 tcttccaatt tcactcaaat ttcttattaa ttcctcttga tattcagtga atcttatttt
      961 attagtataa gcagtaaaat atagataatt caccaatcta tttattaaag gaccgggatc
     1021 taattcatat ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc
     1081 agaatattta ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt
     1141 agcatttttt ttcattttag cacttccctg gaattgctcc acctaaaaaa ccacctatag
     1201 acattcctgc aatacaagta gttgccttgg cccaatcgac cgtacattta tttttagtgc
     1261 aatacactcc atttccataa tattttccac tatgagtggt acccccatag ataagttgtg
     1321 tctctttaat agacaaaatt tttaaatgtt tcataatcta atcaccccta aattaatgtt
     1381 tttagactat tattctaaca ttcattaaat ttcaaaaaac aattccttct tgaaacgtag
     1441 caaaataatt cctgaataag aatttaatca cttttaagta tataaactat gtatattctt
     1501 taatattttt tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata
     1561 cctacgatcg atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg
     1621 gacgtttcga agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa
     1681 catggagctt ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc
     1741 tgtcttttta ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg
     1801 ctgcttgctg tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt
     1861 ctgaccaatg gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt
     1921 ttcgaaccat cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt
     1981 atttcagatc atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt
     2041 cgtgtttttt gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta
     2101 ctttccagtc tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac
     2161 cagctcctgg ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg
     2221 aatttacatc catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac
     2281 ctaggtcttc gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc
     2341 aagctgaaac catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat
     2401 ctaatggcgt catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg
     2461 ttgaagcaac ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg
     2521 cagatgaaaa actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga
     2581 tcagtccttg acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg
     2641 gctggttcaa atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc
     2701 cgttgacaaa tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta
     2761 agccgggtgc accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt
     2821 gattgatccc tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt
     2881 gacgacttgt gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa
     2941 tccctaaatt cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc
     3001 ctggaaccat cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca
     3061 ttgtctgcat aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca
     3121 tcaagatccc taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg
     3181 tgacagtatt catcaaatca ttccatagac cttggttata aataaaccat agaccgatta
     3241 atgtgaacaa cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata
     3301 acaagataaa caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg
     3361 cctgtccaat cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg
     3421 tgatattttc tacccagttt gccactggta attgatagtt caatagatta ttcatgttca
     3481 ctcacctcgc ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt
     3541 aattttccat cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata
     3601 tcacttacca ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat
     3661 ggacgttttt gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt
     3721 ctctttctgt caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca
     3781 acgtttggtc catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc
     3841 gtaagcactt tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga
     3901 ttggtcaata tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg
     3961 attctgtccc caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt
     4021 tgaacattgg cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca
     4081 agagccgaga acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct
     4141 aggccaaccc gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa
     4201 agcaaactag aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca
     4261 ccacggactt ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg
     4321 aagttttgga aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt
     4381 aacttcgata catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga
     4441 ttcaatagac ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt
     4501 tctccttctt ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt
     4561 aaaatctccg ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta
     4621 ccaaatatct ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat
     4681 gtaaattcat ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact
     4741 ttgtcaaaaa tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt
     4801 aaataaaagg tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc
     4861 tgatgctccg ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa
     4921 tgaacgatgt cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca
     4981 ttatgagaaa ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt
     5041 gaacggtcca gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc
     5101 atcttatgta ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc
     5161 agctcatctt gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat
     5221 tgtacatctt gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc
     5281 ccacttccat gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg
     5341 gtagtctcag gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca
     5401 cccactttat atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag
     5461 cgcgggcgga cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc
     5521 cctcatctta ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg
     5581 actgatcatc gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa
     5641 ttcttaccgg attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca
     5701 cttaataaga atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg
     5761 gttcttggat tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc
     5821 aagatacgtc cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac
     5881 aaaactaact aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt
     5941 ggcattcatt tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt
     6001 gaaggctaac aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat
     6061 ttctctgcca ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat
     6121 attttcttta gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc
     6181 aagcggctga aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc
     6241 agaaacaggt tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac
     6301 aaagataatc gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag
     6361 gaccgctgac cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc
     6421 tccagcaaac aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc
     6481 aagaaaatag gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa
     6541 ggcaataatc ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg
     6601 acgatcttgc tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt
     6661 agcaatcagc ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc
     6721 catgacaaaa gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa
     6781 caatataatc aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt
     6841 caagatcagc tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg
     6901 ttctggtaac catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac
     6961 tgtttgaata gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa
     7021 ccaacgattc gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt
     7081 tgacacaaca cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat
     7141 cgtctcacta tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt
     7201 ttgctgagaa actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt
     7261 cattgcatac cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa
     7321 agctgcttct aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc
     7381 agactcctga atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg
     7441 taaaactggt acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt
     7501 tttgatgaca tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg
     7561 cgtagttaaa taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc
     7621 aggctgttcc tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa
     7681 agaatagatg ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc
     7741 ttttttttct actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag
     7801 attgaacaag ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt
     7861 tcctcccttt gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct
     7921 cgcactgtca gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca
     7981 tttcgcgtca atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct
     8041 acgacgatcc cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa
     8101 gcagtctttt tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga
     8161 ctttttacca gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg
     8221 gcaacgatcc cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg
     8281 acttgataaa cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg
     8341 tgaaataaat catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc
     8401 aaataaatga gaatcattct cacatagaat actctcggta attgagaatg tcaattatac
     8461 ttgtgatatt atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct
     8521 tcatagtttt ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt
     8581 ttcagctctt ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg
     8641 tagtgtttgt tgtttgactt ttttactaag agttttatac tatctattat agataataag
     8701 aatgtgttag aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg
     8761 caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat
     8821 atcgatgaac agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct
     8881 gtcattactt ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt
     8941 gccagctaac atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca
     9001 gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga
     9061 tttgcaatca agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa
     9121 atttacaaga tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc
     9181 aaatcccttt gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac
     9241 caaatacgat gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat
     9301 atatagaagc agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat
     9361 ttttggacga gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc
     9421 aatatgcgtt gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag
     9481 ccttcaatgc gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg
     9541 aaatgatcca tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac
     9601 gtcggggcga attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg
     9661 ggcgctcaat caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt
     9721 tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg
     9781 tcgttccagt catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc
     9841 gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt
     9901 cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga
     9961 tcgtacctga taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc
    10021 catatagtgt cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt
    10081 atgatcatct agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg
    10141 aaaatgaaat ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata
    10201 cagcgaaccg cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga
    10261 ttttacaagt tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa
    10321 cggatatctc atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact
    10381 ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata
    10441 tcgatgtcgt accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg
    10501 ccatgggctt acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat
    10561 tagactttac aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca
    10621 atatcgctcg tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg
    10681 gcacttattt cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag
    10741 cattgtttga aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag
    10801 taatgaaaga tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt
    10861 catatatcaa cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc
    10921 aagagaagaa aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc
    10981 cgtattacac ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg
    11041 cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag
    11101 aaggcattta tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa
    11161 gacattacgc tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg
    11221 atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga
    11281 ttcgcttatt catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca
    11341 ataaactgga atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag
    11401 caattttggc tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg
    11461 tctcaattgg aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact
    11521 gtccaatctg aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa
    11581 gccgttttga acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt
    11641 tttagtacac tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat
    11701 ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta
    11761 gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact
    11821 cctctttatt atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc
    11881 atccgagatg aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac
    11941 gagttacctg aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag
    12001 ctttacgaaa acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa
    12061 gaagtcaaga cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat
    12121 ccactctttc cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca
    12181 ggcacaagca accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta
    12241 gaagcgatga aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa
    12301 aaatttttcg aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc
    12361 ttttttatgc ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa
    12421 catgttccat tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca
    12481 ctgcatagac aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa
    12541 tcaataccaa gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct
    12601 tgatcaaatt tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt
    12661 gaaatggaga gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga
    12721 catacgggat aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat
    12781 cgaatattct cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac
    12841 tgctgatata ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac
    12901 gaatcgcttt cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca
    12961 taaatagcaa aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa
    13021 tgatttttga gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag
    13081 ataaatttgc tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg
    13141 ccaattcatt taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt
    13201 gaattgtctg ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga
    13261 ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa
    13321 aaacaacgat tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa
    13381 ctgccgaaag cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca
    13441 cgccgatgat cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac
    13501 gtgattgcat acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga
    13561 gttacgcgct gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg
    13621 agtagacgag tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt
    13681 ccctttttcc aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac
    13741 gaaaaatata aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat
    13801 tttacaacct cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt
    13861 gtgtacggac aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt
    13921 atagatattt ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc
    13981 gattacgata tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg
    14041 gataaattgg atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt
    14101 gagtgcaata gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat
    14161 ggcgatccct aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga
    14221 ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc
    14281 aatcagcctt gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc
    14341 tcttaatgca ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca
    14401 aactaaaata tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat
    14461 tgctccgcca aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac
    14521 catatcattc atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa
    14581 aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc
    14641 tttttatgaa taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa
    14701 atatggggag ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac
    14761 aacctcttta gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca
    14821 caacaacatt gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt
    14881 caatcatccc tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt
    14941 tacttttcat aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt
    15001 tatcgaagaa tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg
    15061 ttccgtcaaa tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta
    15121 agatttggat atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt
    15181 ccagatcttc tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata
    15241 attggacatc catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact
    15301 catctaagtt tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat
    15361 ataaagtaac tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac
    15421 cttctttgat gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga
    15481 tttccatagt gattgctcct ttatattttg gtatagccca attatagcat tctttttttc
    15541 tttttacaat aattagtata gaccaaattg catttttttc attttcttga caataaatag
    15601 gaatccgtgt taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca
    15661 agcaagtgat tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt
    15721 atgagtatac tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa
    15781 ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc
    15841 ggtaaaagta cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc
    15901 aattggcaaa aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca
    15961 ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa
    16021 attgcttttc tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct
    16081 gggaaactcc aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct
    16141 gagatgagca gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta
    16201 agtggtggac aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt
    16261 ctgatcttag atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag
    16321 tttcttcagg attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat
    16381 cagatcacca cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat
    16441 ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat
    16501 gctcaacaaa aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc
    16561 cgatcaacga tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct
    16621 ccagcttcag caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta
    16681 gcagtggaaa caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat
    16741 ttgaccatcc gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct
    16801 actttattaa aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca
    16861 gataacacaa aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca
    16921 ccactacaat atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa
    16981 ctagctaaag cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt
    17041 ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt
    17101 ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata
    17161 cagaaatttc caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt
    17221 gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag
    17281 gagctgtgac aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct
    17341 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt
    17401 cttattactt gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga
    17461 gatttctctt tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa
    17521 gcccgctaac cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg
    17581 aaccagccgc acctccaata aagtacataa acataaatac agtattattt cggttgcttg
    17641 ctgcttcacc tagcccttgt actcgtgttt gattggcgac ttgaccaaat tgattcccga
    17701 cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag
    17761 caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag
    17821 aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta
    17881 atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg
    17941 tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta
    18001 atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag
    18061 ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc
    18121 cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac
    18181 cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga
    18241 ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca
    18301 cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa
    18361 aaaaagcggc caacagagag acggcagata acgctgccat tcgaatgatc aattttggac
    18421 gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg
    18481 tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg
    18541 ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata
    18601 gtaaaataag cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt
    18661 tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc
    18721 acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga
    18781 caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc
    18841 cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca
    18901 cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca
    18961 tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca
    19021 tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc
    19081 gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg
    19141 cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac
    19201 ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat
    19261 gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga
    19321 tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca
    19381 acgagatcaa tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat
    19441 attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct
    19501 cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt
    19561 tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc
    19621 catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga
    19681 taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt
    19741 cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa
    19801 attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact
    19861 cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt
    19921 ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct
    19981 ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga
    20041 agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta
    20101 ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg
    20161 attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat
    20221 acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg
    20281 ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca
    20341 actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt
    20401 acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac
    20461 ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac
    20521 tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt
    20581 tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt
    20641 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
    20701 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
    20761 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
    20821 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
    20881 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
    20941 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
    21001 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
    21061 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
    21121 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
    21181 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
    21241 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
    21301 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
    21361 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
    21421 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
    21481 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
    21541 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
    21601 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
    21661 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
    21721 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
    21781 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
    21841 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
    21901 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
    21961 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
    22021 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
    22081 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
    22141 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
    22201 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
    22261 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
    22321 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
    22381 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
    22441 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
    22501 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
    22561 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
    22621 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
    22681 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
    22741 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
    22801 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
    22861 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
    22921 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
    22981 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
    23041 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
    23101 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
    23161 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
    23221 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
    23281 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
    23341 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
    23401 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
    23461 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
    23521 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
    23581 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
    23641 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
    23701 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
    23761 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
    23821 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
    23881 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
    23941 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
    24001 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
    24061 aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg
    24121 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
    24181 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
    24241 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
    24301 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac
    24361 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
    24421 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
    24481 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
    24541 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
    24601 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
    24661 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
    24721 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
    24781 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
    24841 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
    24901 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
    24961 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
    25021 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
    25081 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
    25141 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
    25201 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
    25261 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
    25321 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
    25381 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
    25441 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
    25501 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
    25561 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
    25621 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
    25681 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
    25741 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
    25801 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
    25861 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
    25921 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
    25981 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
    26041 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
    26101 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
    26161 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
    26221 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
    26281 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
    26341 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
    26401 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
    26461 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
    26521 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
    26581 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
    26641 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
    26701 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
    26761 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
    26821 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
    26881 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
    26941 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
    27001 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
    27061 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
    27121 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
    27181 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
    27241 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
    27301 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
    27361 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
    27421 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
    27481 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
    27541 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
    27601 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
    27661 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    27721 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    27781 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    27841 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    27901 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    27961 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    28021 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    28081 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    28141 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    28201 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    28261 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    28321 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    28381 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    28441 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    28501 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    28561 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    28621 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    28681 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    28741 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    28801 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    28861 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    28921 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    28981 ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca agaattaatt
    29041 ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc
    29101 taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg
    29161 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
    29221 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
    29281 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
    29341 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
    29401 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
    29461 aattcccagt tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc
    29521 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
    29581 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    29641 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
    29701 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
    29761 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
    29821 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
    29881 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
    29941 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
    30001 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
    30061 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
    30121 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
    30181 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
    30241 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
    30301 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
    30361 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
    30421 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
    30481 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
    30541 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
    30601 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
    30661 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
    30721 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
    30781 gattaaatgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
    30841 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
    30901 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
    30961 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
    31021 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc
    31081 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
    31141 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
    31201 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
    31261 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
    31321 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
    31381 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
    31441 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
    31501 gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca
    31561 acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca
    31621 aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt
    31681 tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg
    31741 gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag
    31801 acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg
    31861 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
    31921 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
    31981 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
    32041 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
    32101 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
    32161 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
    32221 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
    32281 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
    32341 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga
    32401 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
    32461 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
    32521 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
    32581 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
    32641 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
    32701 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt
    32761 ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag
    32821 attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa
    32881 gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca
    32941 gctccttgat actttctaaa attttattcc gactttgggg gtctacagct actgtcggct
    33001 catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt
    33061 tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt
    33121 gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa
    33181 aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga
    33241 ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa
    33301 tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac
    33361 tgccgtttgg tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat
    33421 tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga
    33481 aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag
    33541 tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa
    33601 tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt
    33661 caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc
    33721 agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt
    33781 gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa
    33841 gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat
    33901 catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga
    33961 aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat
    34021 tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca
    34081 caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat
    34141 gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa
    34201 gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat
    34261 cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta
    34321 gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga
    34381 ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt
    34441 atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat
    34501 cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt
    34561 ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa
    34621 aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag
    34681 tcattattga gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
    34741 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac
    34801 ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc
    34861 tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa
    34921 agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca
    34981 gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg
    35041 taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca
    35101 tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa
    35161 aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat
    35221 cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta
    35281 tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc
    35341 attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga
    35401 aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga
    35461 aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct
    35521 acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca
    35581 ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa
    35641 cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac
    35701 caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa
    35761 tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact
    35821 tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct
    35881 ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca
    35941 ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta
    36001 tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg
    36061 gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc
    36121 cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc
    36181 aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga
    36241 tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt
    36301 gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt
    36361 gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata
    36421 cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg
    36481 aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt
    36541 ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc
    36601 cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt
    36661 tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga
    36721 tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga
    36781 ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt
    36841 tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct
    36901 ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc
    36961 agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc
    37021 gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg
    37081 gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt
    37141 agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct
    37201 gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt
    37261 aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac
    37321 tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa
    37381 tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa
    37441 aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca
    37501 agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct
    37561 aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga
    37621 actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct
    37681 gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct
    37741 tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg
    37801 tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga
    37861 tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa
    37921 ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc
    37981 agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa
    38041 agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta
    38101 agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt
    38161 tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg
    38221 gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc
    38281 gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt
    38341 cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac
    38401 tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact
    38461 attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat
    38521 ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc
    38581 aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg
    38641 aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata
    38701 tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag
    38761 cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt
    38821 atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt
    38881 tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct
    38941 cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc
    39001 catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag
    39061 cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc
    39121 ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt
    39181 catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt
    39241 tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata
    39301 tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa
    39361 agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg
    39421 ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt
    39481 cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa
    39541 aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca
    39601 atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac
    39661 gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa
    39721 aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa
    39781 aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct
    39841 tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag
    39901 gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac
    39961 gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat
    40021 aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt
    40081 cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt
    40141 aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat
    40201 tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc
    40261 cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat
    40321 tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct
    40381 gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa
    40441 cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt
    40501 ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta
    40561 cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc
    40621 ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg
    40681 ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg
    40741 agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat
    40801 tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc
    40861 gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat
    40921 gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat
    40981 gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct
    41041 tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg
    41101 gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc
    41161 aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga
    41221 caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg
    41281 cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga
    41341 attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa
    41401 acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata
    41461 aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc
    41521 aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc
    41581 atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct
    41641 atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc
    41701 cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat
    41761 ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt
    41821 ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca
    41881 cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga
    41941 gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc
    42001 acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg
    42061 atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct
    42121 aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact
    42181 gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg
    42241 tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag
    42301 attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga
    42361 ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa
    42421 tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca
    42481 atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa
    42541 agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg
    42601 tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc
    42661 agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc
    42721 tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg
    42781 gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt
    42841 caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact
    42901 cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc
    42961 ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa
    43021 aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt
    43081 ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca
    43141 ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg
    43201 ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac
    43261 gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaagccag
    43321 atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt
    43381 tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag
    43441 acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga
    43501 tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta
    43561 ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt
    43621 gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa
    43681 aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc
    43741 tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa
    43801 tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc
    43861 aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc tagccattca
    43921 tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca
    43981 atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta
    44041 ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca
    44101 aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt
    44161 gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca
    44221 tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct
    44281 tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag
    44341 aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct
    44401 ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct
    44461 aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc
    44521 gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta
    44581 gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc
    44641 acggatactg gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg
    44701 ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt
    44761 tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc
    44821 gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt
    44881 tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat
    44941 ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc
    45001 agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat
    45061 actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc
    45121 acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata
    45181 tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt
    45241 cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca
    45301 aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga
    45361 aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca
    45421 attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca
    45481 gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac
    45541 attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga
    45601 agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact
    45661 ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg
    45721 agctgaattc cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct
    45781 acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat
    45841 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    45901 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    45961 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    46021 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    46081 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    46141 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    46201 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    46261 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    46321 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    46381 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    46441 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    46501 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    46561 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    46621 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    46681 aagaactagc catttacact tgctttctaa ttaaaaaagg ctctttgtca aatgatgttg
    46741 gtaaagagaa atcttagaat a
//
LOCUS       DAFBYI010000008        46472 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFBYI010000008 DAFBYI010000000
VERSION     DAFBYI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.008"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            116..970
                     /locus_tag="LLD04_000378"
     CDS             116..970
                     /locus_tag="LLD04_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK5647337.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            971..2308
                     /locus_tag="LLD04_000379"
     CDS             971..2308
                     /locus_tag="LLD04_000379"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK5647338.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2313..2537
                     /locus_tag="LLD04_000380"
     CDS             2313..2537
                     /locus_tag="LLD04_000380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK5647339.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2539..3429
                     /locus_tag="LLD04_000381"
     CDS             2539..3429
                     /locus_tag="LLD04_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5647340.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3438..4256
                     /locus_tag="LLD04_000382"
     CDS             3438..4256
                     /locus_tag="LLD04_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK5647341.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4363..4812
                     /locus_tag="LLD04_000383"
     CDS             4363..4812
                     /locus_tag="LLD04_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5647342.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4830..6512
                     /gene="recN"
                     /locus_tag="LLD04_000384"
     CDS             4830..6512
                     /gene="recN"
                     /locus_tag="LLD04_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK5647343.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6567..6683)
                     /locus_tag="LLD04_000385"
     CDS             complement(6567..6683)
                     /locus_tag="LLD04_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK5647344.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6756..7712)
                     /locus_tag="LLD04_000386"
     CDS             complement(6756..7712)
                     /locus_tag="LLD04_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5647345.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7883..8251
                     /locus_tag="LLD04_000387"
     CDS             7883..8251
                     /locus_tag="LLD04_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK5647346.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8421..8852
                     /gene="mraZ"
                     /locus_tag="LLD04_000388"
     CDS             8421..8852
                     /gene="mraZ"
                     /locus_tag="LLD04_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK5647347.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8873..9832
                     /gene="rsmH"
                     /locus_tag="LLD04_000389"
     CDS             8873..9832
                     /gene="rsmH"
                     /locus_tag="LLD04_000389"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK5647348.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9837..10238
                     /gene="ftsL"
                     /locus_tag="LLD04_000390"
     CDS             9837..10238
                     /gene="ftsL"
                     /locus_tag="LLD04_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK5647349.1"
                     /translation="MGELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10239..12431
                     /locus_tag="LLD04_000391"
     CDS             10239..12431
                     /locus_tag="LLD04_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5647350.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12457..13419
                     /locus_tag="LLD04_000392"
     CDS             12457..13419
                     /locus_tag="LLD04_000392"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK5647351.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13433..14794
                     /locus_tag="LLD04_000393"
     CDS             13433..14794
                     /locus_tag="LLD04_000393"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK5647352.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14820..15908
                     /gene="murG"
                     /locus_tag="LLD04_000394"
     CDS             14820..15908
                     /gene="murG"
                     /locus_tag="LLD04_000394"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK5647353.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15923..17143
                     /locus_tag="LLD04_000395"
     CDS             15923..17143
                     /locus_tag="LLD04_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK5647354.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17299..18624
                     /gene="ftsA"
                     /locus_tag="LLD04_000396"
     CDS             17299..18624
                     /gene="ftsA"
                     /locus_tag="LLD04_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK5647355.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18647..19888
                     /gene="ftsZ"
                     /locus_tag="LLD04_000397"
     CDS             18647..19888
                     /gene="ftsZ"
                     /locus_tag="LLD04_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK5647356.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19905..20582
                     /locus_tag="LLD04_000398"
     CDS             19905..20582
                     /locus_tag="LLD04_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK5647357.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20596..21195
                     /locus_tag="LLD04_000399"
     CDS             20596..21195
                     /locus_tag="LLD04_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK5647358.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21210..21497
                     /locus_tag="LLD04_000400"
     CDS             21210..21497
                     /locus_tag="LLD04_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK5647359.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21546..22328
                     /locus_tag="LLD04_000401"
     CDS             21546..22328
                     /locus_tag="LLD04_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK5647360.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22349..23056
                     /locus_tag="LLD04_000402"
     CDS             22349..23056
                     /locus_tag="LLD04_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK5647361.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23318..26116
                     /gene="ileS"
                     /locus_tag="LLD04_000403"
     CDS             23318..26116
                     /gene="ileS"
                     /locus_tag="LLD04_000403"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK5647362.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26165..27691)
                     /locus_tag="LLD04_000404"
     CDS             complement(26165..27691)
                     /locus_tag="LLD04_000404"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK5647363.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27706..28353)
                     /locus_tag="LLD04_000405"
     CDS             complement(27706..28353)
                     /locus_tag="LLD04_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK5647364.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28537..28866)
                     /locus_tag="LLD04_000406"
     CDS             complement(28537..28866)
                     /locus_tag="LLD04_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK5647365.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29043..29771
                     /gene="sfsA"
                     /locus_tag="LLD04_000407"
     CDS             29043..29771
                     /gene="sfsA"
                     /locus_tag="LLD04_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK5647366.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29787..30800
                     /locus_tag="LLD04_000408"
     CDS             29787..30800
                     /locus_tag="LLD04_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5647367.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30800..32077
                     /locus_tag="LLD04_000409"
     CDS             30800..32077
                     /locus_tag="LLD04_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5647368.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32140..34842
                     /locus_tag="LLD04_000410"
     CDS             32140..34842
                     /locus_tag="LLD04_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5647369.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34994..35311
                     /locus_tag="LLD04_000411"
     CDS             34994..35311
                     /locus_tag="LLD04_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647370.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35341..35661
                     /locus_tag="LLD04_000412"
     CDS             35341..35661
                     /locus_tag="LLD04_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5647371.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35747..37207
                     /locus_tag="LLD04_000413"
     CDS             35747..37207
                     /locus_tag="LLD04_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5647372.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37275..37496
                     /locus_tag="LLD04_000414"
     CDS             37275..37496
                     /locus_tag="LLD04_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647373.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37527..37709
                     /locus_tag="LLD04_000415"
     CDS             37527..37709
                     /locus_tag="LLD04_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK5647374.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37709..38122
                     /locus_tag="LLD04_000416"
     CDS             37709..38122
                     /locus_tag="LLD04_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK5647375.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38245..39426
                     /locus_tag="LLD04_000417"
     CDS             38245..39426
                     /locus_tag="LLD04_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647376.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39497..39661)
                     /locus_tag="LLD04_000418"
     CDS             complement(39497..39661)
                     /locus_tag="LLD04_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647377.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39815..39888
                     /locus_tag="LLD04_000419"
     tRNA            39815..39888
                     /locus_tag="LLD04_000419"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39849..39851,aa:Arg,seq:cct)
     gene            complement(39957..41096)
                     /locus_tag="LLD04_000420"
     CDS             complement(39957..41096)
                     /locus_tag="LLD04_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5647378.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(41210..41794)
                     /locus_tag="LLD04_000421"
     CDS             complement(41210..41794)
                     /locus_tag="LLD04_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBK5647379.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(41921..42352)
                     /locus_tag="LLD04_000422"
     CDS             complement(41921..42352)
                     /locus_tag="LLD04_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5647380.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(42370..42690)
                     /locus_tag="LLD04_000423"
     CDS             complement(42370..42690)
                     /locus_tag="LLD04_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5647381.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            42984..43124
                     /locus_tag="LLD04_000424"
     CDS             42984..43124
                     /locus_tag="LLD04_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647382.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(43121..43300)
                     /locus_tag="LLD04_000425"
     CDS             complement(43121..43300)
                     /locus_tag="LLD04_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647383.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            43381..44127
                     /locus_tag="LLD04_000426"
     CDS             43381..44127
                     /locus_tag="LLD04_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK5647384.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            44142..44345
                     /locus_tag="LLD04_000427"
     CDS             44142..44345
                     /locus_tag="LLD04_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647385.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            44361..44699
                     /locus_tag="LLD04_000428"
     CDS             44361..44699
                     /locus_tag="LLD04_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK5647386.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            44686..44865
                     /locus_tag="LLD04_000429"
     CDS             44686..44865
                     /locus_tag="LLD04_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647387.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(44985..45563)
                     /locus_tag="LLD04_000430"
     CDS             complement(44985..45563)
                     /locus_tag="LLD04_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647388.1"
                     /translation="MVVFSMNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEK
                     VINDLKRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDI
                     LEENFRIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRI
                     FSLVIWVTSLFAQETISQILSFVITHFFKGQK"
     gene            45602..46300
                     /locus_tag="LLD04_000431"
     CDS             45602..46300
                     /locus_tag="LLD04_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK5647389.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
BASE COUNT        15565 a         7885 c         9679 g        13343 t
ORIGIN      
        1 atcagaagct ggggcgtaag tcctagcttt tttctatgaa tcatcataaa cctgttataa
       61 atcgggaaaa ttctatggta gaatgaaaac acaaaaaata tctgggagtg gttagatggc
      121 agaattgatt aacggaaaag aattagctga aaaaatgcag gcagagattg ctgaaaaaat
      181 ccaaaaattg aaagataatg gtatccatcc cggattagtc gttttattgg taggggaaaa
      241 tccagccagc caaatctatg taagaaataa agaacgtgta gcaaaagaaa tcggaatcca
      301 ttcattggta gagcgttatc ctgagacgat ttctgaagac gaactacttg cagaaattga
      361 aaaatacaat caagatccta gttttcatgg tatattggtc caattaccat tgccaaagca
      421 tatagatgaa gaaaagatcc tgttagctat ccgccctgaa aaagacgtag acggttttca
      481 tccgatgaat ttaggcagac tccttgcggg taaaccggac aagattcctt gtactccata
      541 cggcatcatg aaaatgtttg aagcttattc tatcgatccg gcgggtaaac gagcagtagt
      601 tatcggacga agcaatatcg tcggtaaacc gatggctcag ctactcttga tggcagatgc
      661 aactgttact gttgctcatt ctaaaaccga aaatctggct gaactggcac aagaagcaga
      721 tattttagtc gtagcaatag gacgtggaca ttttgtcaca aaagaattcg taaaaccagg
      781 tgctgtagtg atagatgtag ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt
      841 gaaatttgat gaagtggagc ctattgcagg ctggatcact cctgtgccaa agggtgtagg
      901 gccaatgact atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag cttttggagc
      961 aggtgaatag atggcacaag aatatttaac tgtcactaca ttaacgaagt atttgaaaag
     1021 aaaatttgat gccgatcctt atttagaacg cgtatattta acaggcgaaa tatcgaattt
     1081 ccgtttacgt gccaatgccc atcaatattt cagcttaaaa gatgaccatg caaaaatatc
     1141 tgctattatg ttcaaaggag cattccaaaa attacgattc cagccaaaag aaggaatgaa
     1201 agtccttgtc gttgggcgta tttcattata tgaagcggga ggttcttatc aaatctatgt
     1261 agagcacatg gagcctgatg gaataggtgc tttatatcaa gaattggaag aacgaaaaga
     1321 aaagctgatg aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa
     1381 gcgaatcgcg gtccttacca gtccaagcgg agcggttatc cgcgatatca tcacgacggt
     1441 gaaaagaaga tatccaattg tccaattggt tttgttccca acagttgtac aaggaaacca
     1501 agcagcggac aatgttgtca ggaatatcca gagagtagaa gagatgggca atttcgatac
     1561 aatgatcatc ggtcgtgggg gtggttcgat tgaggattta tggccgttta atgaagagcg
     1621 ggttgctcgg gcgatttatc aagctaatac gccggtgatt tcatctgtag gacatgaaac
     1681 agataccacg atcgctgatt tagtagcaga tgttcgggct gccactccaa cagcagctgc
     1741 agaattagct gttcctgttt tgtcagaaga gttgatgcgc attcaagaac ggcaagctag
     1801 attgcagcag gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg
     1861 acgttcctat gtatttcggc agcctgaaag attgtatgaa ggtcagacca tcaaattaga
     1921 ccagttgaaa cagcgattat atcaagctac acagcaaatt tatcatgcga aagaaaaaca
     1981 aacgatcgca ttaagccacc aattggaaca agtggctccg ctttatcgag ttaaagcagc
     2041 tcgccaagaa actgcttatc tagaaaaacg cttagaagag aaaatccaac aatatatgaa
     2101 acaacagcag cagcgttttg ctcaagctgt tcaatctctt gatttgttga gtccattgaa
     2161 aatcatggga agaggctaca gctatacgac gttaaatgat caagtagtaa aaagtgtcac
     2221 cgagattcat caaggggatt tgctgacgat ccattatcaa gatggaatcg ttaaaggaca
     2281 agtacaagag atagacaaag gagaataaaa atatggaaaa tccaactttt gaagagtcac
     2341 tgcaagaatt agaaaaaatt gtcatgcagt tagaacaagg agacgttcca ttagaatcag
     2401 ctctagattc gtttaaacga ggaatggaat taagcaaaca ttgccaagat acattagcta
     2461 aagcagaaga gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt gattttgacg
     2521 gaaacgagga aagctgagat gagattaact gacttttcag ctattcatct acctgctgta
     2581 gaaaaagaaa tcttgtcttt tttagatgag catacaacgg atagaggact ctatgatgcg
     2641 atgagttatt cagtgaaagc aggcgggaaa agaatacgtc ccttattact tttaacggcc
     2701 gttgcttcct ttgatgagcc tattgatgtt cccgtctatc aagtagcagc tgctttagaa
     2761 atggtgcata cgtattcctt gatccacgat gatttgcctg cgatggacaa tgatgatctt
     2821 cgtagaggaa aacctaccaa tcataaagta ttcggcgaag cattagctat attggccgga
     2881 gatggtttgc ttacaggagc attccagcta atcagtatgg cacacttagg taattctcct
     2941 aagcttctgc tgcttcagca attagctgtt tgtgcgggat cacaaggtat ggttgctggg
     3001 caagctgcag atatcgaggg cgaatcgaaa aagctttctt tagaagaatt agcatttatt
     3061 catgaaagaa agacagggca tttgattcgt tatgccttgc tggctggtgg catattagcg
     3121 aagcaacctg aagaaatctt attgctactt caacgattag ccgaacattt aggtttagct
     3181 tttcagataa gagatgatct actcgatgtg attggtacaa cgaaaacatt agggaaaaca
     3241 gctggtaaag atgaacgtat ggaaaaaaat acttacccgc gtttattagg cctagaaaag
     3301 acacgtgagg cgttggagat tgagctgtta tccgccaaca agatcataga caaattagaa
     3361 gaggacgttt tttcttttga tggcgggctg atgcgccaga tgatcaagca atttgatgtg
     3421 gagaaatgag ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc aaacaaggat
     3481 tatttgaaac aagagaacaa gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa
     3541 aaaacgagcg gtttgataaa ccgggagaaa aaattccagt gagtagtgaa ttaagagtca
     3601 aaggtaaaaa acttccttat gtttctcgag ggggattaaa attagaaaaa gcactcaaag
     3661 catttgatct atctgttgaa ggaaaaacat tgttagatat tggtgcatcc actggcggat
     3721 ttactgatgc agctttgcaa aatggcgcaa aaatgagtta tgctttagac gttggctaca
     3781 atcagctagc ttggaaaata agacaagatc ctagagtcgt cgtaatggaa agagtgaatt
     3841 ttcgttatgc aaaacccgaa gactttacct taggaattcc agaagttgct gttatcgatg
     3901 tttcttttat ttcactaaaa ctgatgctgc ctccattaca tgctatttta aaagaagagg
     3961 gagaagtgat tgctttaatc aaaccccaat tcgaagcagg cagagaagct gtagggaaaa
     4021 acggaattgt tcgtgaccct cagacccatc aaaaagtttt agaagacatt ttgtcatttg
     4081 cggcacaagg tggatatgat gtgctggaat taagttattc tccgatcacg gggggagaag
     4141 gaaatatcga gttcttggct cacttgagga aagtacccga aagcggaaca atcaattcag
     4201 ctattaatat ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat aaataaattg
     4261 taactgtgaa aatcaagtga gaagtatgcg gattcttctt tattatacca aggaaataag
     4321 ctatcattga ttaagaagaa tatgctaagg agcgaagacg taatgagaaa aaaagatcgg
     4381 catcgtttga ttactcgatt gttgagtgag caagatatcc gaaagcagga agaatttgtt
     4441 gaaatcctga aaagcaaagg aatatctgtt actcaagcta cgatttccag agatataaag
     4501 gaactgaaac tgatcaaggt accggcttca gatggcggct accgttatag cataccagct
     4561 gaaacaaacg aagatgtaaa tactaaattg gaaaaactat taaaagacgc tttcgtggca
     4621 gttgaccaaa tggaaaaatt tgttattttg aaaacattgc cgggaaatgc ttcggcagca
     4681 gcaaatttga ttgataaacg ttacaagaaa gaattatttt cgattatcaa tgatgacgat
     4741 aatgttttga tgattacacg aactgaagac caggcagttt tattgaagaa agacttcctg
     4801 cattaccttt aagttgagga gcgaatgtga tgctacaaga aatcagtatt actaactttg
     4861 cgattattcc ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg accggagaga
     4921 ctggtgcggg taaatccatt attatcgatg ctttaggtct gttagcagga ggaagaggtt
     4981 caagcgatta tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg tttgaactac
     5041 caaaacaaga aggattctct gaactgatgg ttgaattagg gatcgaaaca gatgaggaca
     5101 atctgattgt tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc
     5161 atattatcac attagctaat ctgagaagaa tcggcagtta tttagtagat atccaaggac
     5221 aaaacgaaca tcaggaactg ctccagcctg aatcacatct tgctttattg gatcgttttg
     5281 gagatgtagc tttccaacag aagaaaaaga gttatcagca agaatatatc tcttatcgtg
     5341 agctggaaaa aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag
     5401 atatgctcca tttccagcaa gaagaaatag ctgctgctga tttgcaagtc ggagaagaag
     5461 agaaattaaa agaagaaaga gaaaaactta gcaattatca aaaaattgct gatggattag
     5521 ctgcaggtta cggagcactg acgaataatg aacaaaacag tgtagatggt gttggcttgg
     5581 ccgtttcaga actgcaaggg atcgcccatc tagatgtgga gtatgaagca atttatgaaa
     5641 atatccaaag tgcttactat ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg
     5701 atttattgga gttagatgag agtcgtttag aagaagttac gcaacgttta gaattaatcc
     5761 gacagttgaa acgtaaatac ggcgaatcca ttgagtcaat attggcttac tatgacgaaa
     5821 tcaccgaaga gctagcttct tctgattttt cagaaggtca attggacaaa atgaaagagg
     5881 aactagagca aaaagagctt ctacttcagc aacaagccgc agatcttcat gaagcgcgta
     5941 aaaaaatcgc aaaagaattg gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg
     6001 aaaacactga gtttgaggta cgtttcttaa aagaagaaaa tagacaattg aatcatgatg
     6061 gatttgacca aatcgaattt tacattacga ccaatccagg agagccactg aagcctttgg
     6121 tgaaagtcgc ttctggtggc gaactgtctc ggatgttgct tgctttgaaa actatttttt
     6181 cttctgagca aggtgtgaca agtattattt ttgatgaagt ggatacaggt gtgagcggaa
     6241 gagttgcaca ggccatcgca gataaaattt ctcaaatatc gaaatactct caagttttgt
     6301 gcatcactca tctgcctcaa gttgctgcag tcgccgacta tcaatattat attgtgaaag
     6361 cagtgattga tggacgtacg caaacttctg tttcagagtt gaaaacaaaa gaaagagaag
     6421 aagaaattgc acgtatgctg gctggaagtg agattacaaa actcacactc gaacatgcaa
     6481 aagaactatt gaaactcgca aaaaggacct gagccacctc gcagtggttc aggtcctttt
     6541 cagcagctga tagctatatt ttatttttac atgacactca ttacagcagt caatacaaca
     6601 gatccaatcg ttaaaatcag cattgtccaa atgacaaatt ttgttacttt agcaaaagta
     6661 cttgtttgtt ttttttcacg cattgttttc aacctcgtca ttaattagtt gattcaagtg
     6721 tacaatcttt tttccatttc cacaagaggg cttttttata aaaacttccc tttttttaaa
     6781 taataggtag ttacgataca aataaataat gttaatagaa ctagccatat aaaggcatca
     6841 tccctttcag caataggtaa cttaatgttc attccataga tactgccgac gattgtcggt
     6901 atagttaaaa taatcgttaa agaagttaaa attttcataa tattattcag attattcgat
     6961 acgattgctg aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta
     7021 gtcattgcct gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt
     7081 tcttggaaaa gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct
     7141 tctctgaaat aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca
     7201 gtggatactt gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa
     7261 ctgttgtaag agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa
     7321 atatcacttt ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc
     7381 gtaattgtga taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact
     7441 tgaaaataac cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc
     7501 tttgtcaact ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg
     7561 gaagtgatgt aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga
     7621 ttaggacgtt ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt
     7681 ttctcattat ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt
     7741 attataacgg atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact
     7801 gcataaattt aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac
     7861 gataaaaaag gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat
     7921 ttacccggta atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg
     7981 tcgttttaaa ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc
     8041 attgtctaga aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct
     8101 aatgggaatt tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg
     8161 ttatttcaag atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt
     8221 gatcattgtg aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtcttt
     8281 taaaaggctt tttgcagctt gatttttaaa aaaatgttta tgaataaaaa aagttgttcc
     8341 cggatttccc agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt
     8401 ggaggaatgg ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag
     8461 gacgtttaat cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac
     8521 gcggattgga tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta
     8581 aactgaacga aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt
     8641 cggctgccac tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa
     8701 gaaattatgc agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa
     8761 tttgggatga gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa
     8821 ttgctgaaag tatgattgat ttcggactat aagaatagga agaggctgac taatgactga
     8881 aacatttcaa cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc
     8941 ggatggcgtt tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag
     9001 ccaactaagt tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca
     9061 cgccaagctg cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa
     9121 cttcagagaa ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta
     9181 tgatctaggg gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca
     9241 agatgcaccg ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat
     9301 taatgaatat tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt
     9361 ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac
     9421 tggtgagctt gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg
     9481 gcatcctgca aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc
     9541 cgaagaggcc tctttagaac aggcaatcgg gttattgaaa gtcaatggaa gaatcagcgt
     9601 aatcacattc cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac
     9661 ggtacaagat ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa
     9721 agtcattact cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc
     9781 acgcagtgca aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg
     9841 gagaattaaa gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg
     9901 aacaacaaat gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa
     9961 aacgattaaa gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta
    10021 ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag
    10081 aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac
    10141 aagaaaaaaa cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat
    10201 tgtctattaa tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc
    10261 ggcggtttat ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca
    10321 ttttatttgc tacgagtatt ggattattct ttttatttgc cgtccgtttc tcgtatatcg
    10381 ttattggagg tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag
    10441 gaagtgaagt agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg
    10501 cagaagatgc ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg
    10561 ataaaaaact gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga
    10621 atctttctat agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc
    10681 tgtatcaagt tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca
    10741 tcgaagcgga catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc
    10801 ggatgtatcc aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag
    10861 atgagaatgg cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg
    10921 gtaaagatgg taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag
    10981 tggcagaaga agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac
    11041 tacaaagtta tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat
    11101 tgacagcagt cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta
    11161 ctttcaatcc tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct
    11221 tggtacaaga tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta
    11281 tcgaagaagg agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag
    11341 atgctacgat caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc
    11401 tttcatggtc aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt
    11461 ataactattt acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag
    11521 taaacggtgc tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag
    11581 cggtaggtgt gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta
    11641 caatgattca acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa
    11701 caactcaaac agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg
    11761 aatatatgag agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg
    11821 tacctggata caatgtatcg gcaaaaaccg gaacagccca aatcgcttct gatacagggg
    11881 gatatcaaac aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag
    11941 atccagacta tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat
    12001 tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag
    12061 attcagatac agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag
    12121 cggacgttgc agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg
    12181 gtaaaaaagt acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac
    12241 tgatcctata tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag
    12301 atatcatgaa gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt
    12361 gtgtcaaaca agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt
    12421 tagaagaata agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt
    12481 cccattgtca gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt
    12541 caaatgaaaa agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa
    12601 gccggaactc ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt
    12661 tgggttggag cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta
    12721 gcgttatacg gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac
    12781 atgggactga actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat
    12841 cttgtttacc gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct
    12901 ttaggaatca tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta
    12961 aatctgacag atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct
    13021 tacggtctaa ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt
    13081 ttaggaggtt tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga
    13141 gatgtcggtt cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa
    13201 gaatggacat tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt
    13261 caagttactt cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat
    13321 cactttgaaa tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc
    13381 attgtgactt cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa
    13441 aatatcaaca tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag
    13501 tgcggccaaa ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt
    13561 tgacgaaaat ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag
    13621 tcatccaatc gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc
    13681 ctacagtcat cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt
    13741 tgagctagct tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa
    13801 aacaacgaca acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc
    13861 acgcttagcg ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga
    13921 agataaaatt gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc
    13981 tcatatagcg gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa
    14041 agaatatgtc aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat
    14101 ccttaactgg aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact
    14161 gcctttctcg acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata
    14221 ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt
    14281 agaaaatgca ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat
    14341 acgagaaacg ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat
    14401 ccaaggcaga aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc
    14461 tctaagtgga tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa
    14521 tacatttgat gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca
    14581 aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc
    14641 cgattctgta gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt
    14701 tttattatca ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa
    14761 cagattcatg gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa
    14821 tgaaaatttt ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg
    14881 tcaattatgt aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag
    14941 gtctagaaaa caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag
    15001 gatttaaacg aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta
    15061 tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag
    15121 gatatgtttc cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc
    15181 atgaacaaaa cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga
    15241 tcgctctttc atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag
    15301 gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg
    15361 atttggatcc agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa
    15421 tcaatcaagc agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt
    15481 atgcttcagg agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa
    15541 atattagtgt ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc
    15601 tggtggggcg tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag
    15661 tgttggttcc cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg
    15721 ttcacgcagg agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc
    15781 agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta
    15841 aagaacaagg aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca
    15901 aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc
    15961 gtcttctgat gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga
    16021 acaagagatg aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc
    16081 agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa
    16141 aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc
    16201 ttttttgaac cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt
    16261 agctttgatc atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc
    16321 ttatagcaaa ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat
    16381 catctctgat acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc
    16441 tacttatatc gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt
    16501 caaagggatg aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc
    16561 aaaaaacaat caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc
    16621 aaatcctaca aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga
    16681 attaacaaag caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa
    16741 gtacacgcca agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca
    16801 agttattgta aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa
    16861 agatatggat gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg
    16921 tagcactcaa gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa
    16981 tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg
    17041 atcgtctagc acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga
    17101 agataatcca agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa
    17161 gtaagaatgt actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat
    17221 ctattaataa gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag
    17281 taggagggaa tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg
    17341 acatctgtta aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta
    17401 ggaaacgcaa aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc
    17461 caagcgatac aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc
    17521 gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca
    17581 gttaacggag attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc
    17641 cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact
    17701 gtcgatgggt tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg
    17761 tacggtctgt tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa
    17821 aatgccggat taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt
    17881 ctttctgacg gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca
    17941 acaacggctg ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc
    18001 gaatttgtca ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg
    18061 ttgaaaataa actacgggga tgcataccca gaacggacat caccagatga agaatttcca
    18121 gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt
    18181 atttcagctc gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa
    18241 gcattagagt tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt
    18301 gtggacttgg cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg
    18361 gggctgagaa acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg
    18421 agtgaagtgt atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa
    18481 atggtagttg aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca
    18541 atctacgatg agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt
    18601 ttctttacaa atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat
    18661 tgataataat atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg
    18721 aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa
    18781 cacagacgtg caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa
    18841 atatactcgt ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga
    18901 aagtgaacaa tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg
    18961 aatgggtgga ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt
    19021 gggcgcattg actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg
    19081 ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat
    19141 ctcaaataac cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg
    19201 tgaagcagat aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc
    19261 aggttacgtt aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc
    19321 attgatgggg atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc
    19381 aatctcctct ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat
    19441 cacaggcggt ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa
    19501 tgcagcaact ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga
    19561 tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc
    19621 tagccgacca gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat
    19681 ggatcaagct aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag
    19741 acgtgaacaa aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa
    19801 agaatttgat acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac
    19861 gccaccgttt ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact
    19921 tgcagaaaat caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag
    19981 aagttacatt agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta
    20041 gagctggcgt tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag
    20101 atttatttga ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg
    20161 taaaattgat tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag
    20221 aagaaatcca gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg
    20281 gtgaggaatc aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag
    20341 ctgcctgcac aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg
    20401 atgagcaaat ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa
    20461 aaggttatgc ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa
    20521 ttgcgattga agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat
    20581 aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta
    20641 gctgatgagg aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag
    20701 ccaactaaaa aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact
    20761 catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct
    20821 gaaaagaaaa aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga
    20881 acagcttcaa gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg
    20941 gaaccaagag cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca
    21001 accttagtga attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta
    21061 acaggaacag tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt
    21121 tgtacaccga ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat
    21181 ttgtttgatc tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac
    21241 ttcaacgggc agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt
    21301 ttccaggcgg gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata
    21361 tcagtttgtt cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg
    21421 caatcctgat cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg
    21481 tccatttagt ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga
    21541 aaattttgga tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt
    21601 cggtaggcga ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc
    21661 ttgacccaag acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt
    21721 tcatgtttta tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt
    21781 attacgaacc tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca
    21841 aatttgccag cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac
    21901 gctcttactt tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg
    21961 aggtggatca ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg
    22021 aaccaatcaa atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa
    22081 cagttagttc catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc
    22141 ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac
    22201 ctgattttgt acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga
    22261 tccaagaact tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac
    22321 gtaaataaaa taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa
    22381 gacattcccg acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt
    22441 agttgttcgt gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa
    22501 acattctgaa gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat
    22561 catcgtagct caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt
    22621 gatcgttact tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc
    22681 acatcaatta acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga
    22741 aaaagcaaga cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca
    22801 tcaacgtatc agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga
    22861 tgaaatttta caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt
    22921 tttagcaaaa cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat
    22981 agaagcacct atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga
    23041 aaatgaaaac gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt
    23101 ggaagttcgg taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa
    23161 attagagtaa gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag
    23221 atgcaactaa atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt
    23281 ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca
    23341 attaggaaaa acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca
    23401 aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc
    23461 tagctttgtt cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc
    23521 attgaataaa atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc
    23581 accatatgtt ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa
    23641 aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc
    23701 actttcccaa gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg
    23761 ggaacatcct tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg
    23821 taaaatggct gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc
    23881 aagtgagtct tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat
    23941 ttttgtagct ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt
    24001 catttggacg acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa
    24061 atttacgtat gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct
    24121 agaaacagtc caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg
    24181 acaagaaatg gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat
    24241 tttaggcgat catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca
    24301 tggggaagat gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga
    24361 ctcaaaagga ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc
    24421 aaatcctatg atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt
    24481 tactcatagt tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc
    24541 acaatggttt gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt
    24601 agagtgggtt atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga
    24661 ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa
    24721 tggagaagca atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca
    24781 tggatcaatt atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca
    24841 cccaggttct cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga
    24901 ttctggttct tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga
    24961 tatgtattta gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag
    25021 tgtggctatc aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga
    25081 tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat
    25141 caaacaattt ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga
    25201 tgttcgtgtt tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa
    25261 tacgatgcgt ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc
    25321 gtacaatgat ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa
    25381 agaaatccga gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat
    25441 gaacttcttg acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta
    25501 tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc
    25561 tgtagctttg acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc
    25621 attcctgaaa gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt
    25681 tgcaaaccaa gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt
    25741 cttaaaagca ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa
    25801 actaactgtt tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat
    25861 tgcacaatta ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt
    25921 gccagataac gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga
    25981 aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca
    26041 tctatgcggt cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg
    26101 attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt
    26161 gttatcaatc gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt
    26221 cgaatccgtc tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt
    26281 agttgggaaa atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc
    26341 aagattgagc gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta
    26401 atagtaattt ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt
    26461 ccaatttgat tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag
    26521 aaaatccttc agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt
    26581 cacatggttc atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt
    26641 tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg
    26701 accaacggaa gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag
    26761 catccacttt gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag
    26821 catattgccc gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt
    26881 tcaatgcttt gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca
    26941 ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac
    27001 tatgatcgta gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga
    27061 tattgtcgat atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag
    27121 ctgagatgtt ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt
    27181 ctgggaatac ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga
    27241 acggtttttc tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca
    27301 aatgagaaac gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac
    27361 cgtttaattg atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg
    27421 ttgaatcatt tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt
    27481 catctgaagg attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc
    27541 tccagggacg gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt
    27601 aaaggcggaa caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata
    27661 tcgtaaacaa tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac
    27721 aaaaatcgtg ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt
    27781 gatcggtcct tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc
    27841 aatagtgact aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt
    27901 tgtaccgaca ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt
    27961 tttaggaatc gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg
    28021 atctgcaaga tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt
    28081 ccagctatag tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag
    28141 actgctagct ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc
    28201 tacaagctct tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc
    28261 tagactagat acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat
    28321 gattttcaaa taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa
    28381 ccatcattag taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca
    28441 aaggattttt agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt
    28501 cgtttataaa ggatatatat atacattggg agggatctat ttttcagata acaacttata
    28561 tgaagtgtct gcagattcgt cataaagagt ttgttcggga taagaaaaga taagataaaa
    28621 accatactga tcacgtacga ctaaacgata ttcatctatt tctaccacag atgtgccaag
    28681 agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg
    28741 gctatcacta aagaaccatg taccttgata ctttgagagt ccttctgact tttccgcttg
    28801 ttttttcttt ttcaagtaaa taaaacttga gagaacggta atgagtgaac caagaaaata
    28861 gatcattttt tttatcacca aatcactcct ttatgagaaa ataatcgcat agacatattc
    28921 tttcttcttt atttcattgt accgaataaa agctaaaatt tctctaagac tttcgactgg
    28981 tttttttact gtttttctac aaattatgat aggatacata gtaggaagaa aggtgacata
    29041 atttgaagta tcagcaagtg gaaatagctt tttttattga ccgcccgaat cggtttatag
    29101 cacattgtat gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat acaggtagag
    29161 gaaaagaggt cctgatacca ggcgctgaag tggctattgt atttgctcct ggacctaaac
    29221 ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg tgactggttt aatatcgata
    29281 gtcaattgcc taatcggctg gcaattgatg gtattttaga cggcacgatc cttttaccag
    29341 gattaactgg tccatttacg gtatacaagc gtgaagtaac ttatcttcac tccaaatttg
    29401 atatttattt agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac
    29461 ttgaaaataa agcgattggt gcttttcctg atgcgcccac actcagagga ttgaaacata
    29521 taggagaatt aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg tttattgctc
    29581 agttcgaaca tcttcatctg gcaacgatcc atgaagaaat gcaaccagca ctagctgata
    29641 tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc ctataattgt caagtatcgg
    29701 cagatgaggt aactgtcaag caggccattc catttgatgt agatatccct tttgaagatc
    29761 ctatacaata gaaagaaggt aaaataatga tcaacttagg tattatcgga acaaattgga
    29821 ttactcatca atttgtccaa gcagctttaa gcacaaaaaa atatgaattg acagcggtat
    29881 attctagaca tttggaaaaa gcacaggaat ttggttctca ttatgaaggg gatatcgaat
    29941 tcgcaacaga tttgaagact tttttcaata ttactcatat ggacaccatt tatattgctt
    30001 ctccgaatag tcttcacttt agtcaagcta agcaagcaat tttggcagga aaaaatgtga
    30061 tagttgaaaa gccagctttt tcgacgccaa aagaaatgga tgaaatcatc catttggcaa
    30121 atgaacacaa tgtgtatttt tttgaagcag caagaaatat ccatgaatca ggttttaaaa
    30181 agattgccga ttacttacca ttaaaagacg agataatcgg tgcaaatttc agctatatga
    30241 aatattcttc gcgctatgac caagtattag atggagaaga accgaatatc ttctcgactc
    30301 atttctcagg tggtgcattg atggatttag gtgtttacct tgtatatgca gctgttgctt
    30361 ggtttggaat gccaaaagaa gtccattatt tcccaagaaa gatttctact ggtgtagatg
    30421 gtcttggatg ggggatctta cgttatgaga attttgatgt atcgatccag ccaggtaaaa
    30481 tcggtgattc ttatttaccg tcagagattt attttgactc aggaacgcta gtgatgaatg
    30541 gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc gcataaagaa atcattgatt
    30601 tggcggtaac agctgcagaa aatccaatgg cagatgaagc agaggatttt gccaatgtca
    30661 tggctcatcc gaaagatcca gcttggggat tgttgtatga agagtggatc gagctagcta
    30721 gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag
    30781 ataatgagga agatttttaa tgaacgaatt tgtacaagta tttcccgaaa attggcagaa
    30841 aaaatatgag gataaagggt ttatctcacc ttctcttatt caacgagcag tttttcagcc
    30901 actaagtaat aaacaaagta tagtagcggt ttctcctaca ggttcaggta aaacactggc
    30961 ttatctttgg cctctactac taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt
    31021 tgcttcttct caagaactag ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga
    31081 caaagagttg aaagtccaat ctttggtggg cggagccaac gtcaaacgtc agattgaggg
    31141 tttgaaggaa aaaccagaaa tcttgattgg tacaccagga agaatattgg aattgatgaa
    31201 agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta tttgatgaag cagaccagct
    31261 atttgacgct gggaacagcc aaatcattga tcaaattctt catcaagctc caacagagta
    31321 tcagctagct tttttttcag cgacagcaga tcgttccctt gaaagcattg aaaaaatcac
    31381 tgggaaaacg tttgagacaa ttgacgtgac agcagaggac gattctcgaa aaggactgag
    31441 acattacttt ttacgtgtac cgatacgaaa gaaagatgac tacttgagac gtctcacgca
    31501 tatagaagat tttcatggtc tgattttctt taatcaactg actgaattag gcgtaatgga
    31561 agaaaaattg ctgtatagag gtgtaccggt aggaagtctg gcctctgatc agaacaagct
    31621 actccgaaaa gcagcgctag aacaattcaa aaagaaaatg gtcagagcac ttttgaccac
    31681 agatgtggcg gctagaggtt tagatattac aggtcttcct tatgtagtga atgcagaagt
    31741 tccgttaagt gaagaagctt atttgcatcg ttcgggaaga actggtcgaa tgggtaatga
    31801 aggaatagta attacactcg tacaagataa taatctaaga gatttaaaac ggcttttacg
    31861 tccatcaaat ataacacttg aagagatttt tttatacgga ggtaagctgc aaactgagca
    31921 gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg
    31981 aaaggagcag aacccagaat ataacaaaga aaaaataaga aaaaaagtta aaaataaatc
    32041 aaaaaaagag agaaataaag gagcaaggcg caaataagcg cctttttatg ctatgattat
    32101 gaaaacggga acaaaattgg ttgccagagg aggggttatg tgaaagaacg tcaagaagag
    32161 atcttgcagc tattgacaga aaaccaacaa ggattgacag caagtgatgt agctgagcgc
    32221 ttaactatcg atcgaagcaa tgcaagccga tatttaagtg aactatacaa ggcccatcat
    32281 attgtaaaaa ccgctggccg accagtcgtt tactctttgc caacggaaaa gtcaaaatct
    32341 gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg aaacactcgt tggagaaaat
    32401 gattctttaa aagtcagcat acagcaggca aaagctgcga ttctttatcc accgcggggg
    32461 ttgcatacga ttatttttgg agaaactggt acagggaagt cgatgtttgc tgaatgtatg
    32521 tatcattttg caatcgattc agagatgctt tctgcagatg cgccatttgt ttctttcaat
    32581 tgtgcggatt atgctcaaaa tccgcaactg ctctttggac acatctttgg gatcaaaaaa
    32641 ggggcttaca caggagcagc tcaagatagt cccggtctaa tagccaaagc agacggcggt
    32701 atattgtttt tagatgagat ccaccgttta ccgccggaag ggcaggaaat gctgtttact
    32761 ttcattgata aaggtattta ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct
    32821 gttcagatca ttggggcgac gactgaatct tcagaaagtt tcttatctac gttcaatcga
    32881 cggattccta tggcaatcac cttgccgaat cttgcttcac gttcactgga tgagcgttat
    32941 gaaatcatct ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc
    33001 gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg
    33061 aaacgagact tgaaactagt ctgtgcaaaa tcttttttac attaccgtac tcatcatgag
    33121 aaaaagctaa tcattcgaaa agaagaactt tcccttcaag tacaaaaagg attactgaaa
    33181 atcaaggaaa taccggaacg tctcgaccga ttcatggaca gcaaaagtca gtatttgaca
    33241 tttgaaccag gatttgctga tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc
    33301 tataacgata ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga gaacattgat
    33361 ctagagaccc tcatttcaaa agatgtggat gcttatttcc agacatatgt caaagaattg
    33421 actaaaagtt cgattcccaa agatcttttg ccagatgaaa tctggcggtt aaccaatcaa
    33481 ttgtatgatg tggctgaaaa acggttagac cgtatttata atgaaaaagc tcgttttgct
    33541 tttgctttgc atctgcaaag tacattggat cgtgtgagag aaggtcatat gatcgtccac
    33601 ccagatttga ataacgtacg gaaaaatttt aagagggaat tccaggtagc aatcgatctt
    33661 tcaacgatca ttgaagaaga agaacaagtt gagattcctt ttgatgaaat cggctttatt
    33721 agtatgtttc tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa caaagtagat
    33781 gttctcatat tgatgcacgg cagacagact gcttcaagca tgttggagat ggcacaagaa
    33841 ctattaggaa caaaagtagg gaaagctttt aatatgccac tggttatgga agttcaaaca
    33901 atgtatgaag aagtgttaca atatgtcaaa aactatcaag agcatttgac gaatggccta
    33961 ctcttgttaa cagatatggg atcattaaac accttcagtg atttgattta tgaagagact
    34021 ggcattcgga cgaaagctat cacgatgaca agtaccatga ttgtgataga agcgatacgg
    34081 atggcaagtg ttggaagaag cttagaagat atttatcaaa atatccagct ttcctttgaa
    34141 tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc
    34201 gtaatcgtga catgctttac tggtgaagga gtagctgcca aattatatca gcgtattatg
    34261 ccagtgatta atcaaaataa ggtagaactg atccagatgc agtttttaga acgtgaatca
    34321 ttcaaaaaac acatcgatgc cttattggaa gaatatgaga tcaaagcaat tgcgggaaca
    34381 gttgatgttg attatcaaaa tatcccattt ttttcagcct atgacgtttt tgatgatgaa
    34441 aaactaaata ttttaaagcg tatagctgat gatgagatcc cgacagctaa aattgtcgat
    34501 tcacttgctg gatcattaag cgatgtcttt tctattgatg aactgatccg ttcattgcaa
    34561 aagacagttc agcagatcca gaccgatttg tcgattatcg tggaacctgg agtagatact
    34621 ggaatcgtta tccatttagc cttcatgtta gacggattga tcaaacaaga aagccatcgg
    34681 caatttgaga aactgtctat tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg
    34741 gcaaacttga tggcactaga acgaaaatat caggtaaaaa ttccagaaga tgatatcgca
    34801 tttcttacac aaatgtttct tgaaaataaa ataaaaaaat gaacacacta atcatactgt
    34861 ttacacacta aacatctgtg tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta
    34921 tttcagctct tttttttatt ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa
    34981 aggagtgtta aaaatggcaa aaaaaacaat catgttagtt tgttcagcag ggatgagtac
    35041 gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg aagccgatat
    35101 ttttgcggtc tctgcctcag atgcagataa taatttagaa tctaagaatg tcgatgtatt
    35161 attattaggt cctcaagtac gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa
    35221 aggtattcca ttagatgtca tcaatatgca agactatggc atgatgaacg gagaaaaagt
    35281 tcttgcacaa gcagaaaatc taatgaaata aatcaagtat gcagttgggg gtataacaac
    35341 gtggaagatc aacaaaatct agaagccatc atggggttaa tcatgtttgg cggtaatgca
    35401 aagagcgatg caatggaagc gattgcggct gctaaaatcg gtgattttga gttagcggat
    35461 aggaaaatcg ctgatgcaga agaatcactt atacaagcgc atcatgcaca aacagacatg
    35521 ttgacacaag aagcacaagg aaatcatatg caagtgacat tattgactgt tcacagtcaa
    35581 gatcacttaa tgacatctat tgcgtttact gatttagcaa aagaaatcat cgaactttat
    35641 cgtcgcgtat acagcgaata aatacaactt cttttttgaa cggatttttt attttgttat
    35701 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    35761 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    35821 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    35881 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    35941 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    36001 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    36061 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    36121 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    36181 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    36241 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    36301 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    36361 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    36421 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    36481 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    36541 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    36601 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    36661 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    36721 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    36781 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    36841 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    36901 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    36961 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    37021 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    37081 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    37141 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    37201 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    37261 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    37321 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    37381 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    37441 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    37501 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    37561 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    37621 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    37681 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    37741 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    37801 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    37861 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    37921 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    37981 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    38041 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    38101 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    38161 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    38221 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    38281 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    38341 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    38401 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    38461 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    38521 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    38581 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    38641 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    38701 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    38761 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    38821 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    38881 atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat
    38941 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    39001 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    39061 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    39121 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    39181 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    39241 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    39301 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    39361 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    39421 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    39481 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    39541 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    39601 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    39661 ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    39721 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    39781 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    39841 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta caaataaccc
    39901 ctgtatcctt tgtgatacag gggtttgttt tgtgtttgtg ccaaaaatgt gccaaactag
    39961 ttatcatttt tatataaact atcgaacaca gcattgattt tttctttatc ctgatcttca
    40021 agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa
    40081 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    40141 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    40201 tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    40261 tgagggtttt taacttcaaa attctcgaat aattcttttt gattgatttt aaactttttt
    40321 aagagatcaa tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt
    40381 tttccccatt catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg
    40441 aaatcgatat cttcccatgt taatcctaga agttcagcaa atcgcattcc agtagcacca
    40501 gcaactaaca cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc
    40561 attaaagatt taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct
    40621 tttcctttga ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca
    40681 tctttcaacg atgctctaat atggttatta aacttaataa cggtggattt tacatggtct
    40741 ttcgcatact tatttaaaaa gcgttgatat ccaactctat ctaaatcaga aatcaatacc
    40801 gctggcatat atttctttat gttcattaac gtatcttcgt attttctata agtgatagga
    40861 gatactgttc cttctttata aagttgcatc caatcctcaa agtaatctga taataataga
    40921 tttttgcgat cccctttaag gcctttgttc aagttatatt ctaattcgtt ggcggcatct
    40981 tttgcttcgc cttttgtctt aaatcctgat tttcttatct tcgtgtattt tccgtcatct
    41041 tttttgtatg aaatttcgta ttgccaacta tttccacgtt tgactaatct tgccataatt
    41101 gaatctactc tctttctctg atacaatagg cactataaag aagcctactg tataggtttg
    41161 tttttcttag aacacgcttg ctttggacgg tgggcgtgtt ttttatttat tatacagttg
    41221 ctttaaaaat agctgctttt tcaaatgata ctaaaggaga gaagtggatt tctatttctc
    41281 ccttttggtt taacgtgaaa tgagcagtta catccatttg ttttcctgga gcaacagatc
    41341 ccattgtatt ttcgttggca tatgtctctg attttttgtc atctggtcca tatacttcca
    41401 catctgtacc tacagggata tctgaatcgc catcattttt tacggtataa gtaattttta
    41461 ctacttgtgc aggttggttt tcttcaaatt gatttctttc atcagttagt tctacactat
    41521 ttagtgtata ttcagcatca ccaactttta cagtgtcacc aatcttatag aaggtatcgc
    41581 ttttttcttc tttagaagat gaagcagagg ttgaagattt agttactttt tcgccaccat
    41641 tatcacttgc tttattacta tctgatccac catttaaagc agaaccaata attataatta
    41701 aaattactgc taatacccaa aaccatactt ttttgtaaaa aggtttacta actttataca
    41761 tttttccgtc ttgacccata actttttttg ccatttaaat attcctcact tcttgttata
    41821 atatatttgc gatctcagaa atgaggtatg agtccgtgtg ccagcacggg cttttttaat
    41881 gttttgctga aatcggtttt tttaataatt cttgatgtat tcatacatat tgccttgcgt
    41941 aagaatattt tttcttaaaa tagcattggc agacagcata acaagtgtat ctgagcttat
    42001 tctgaataga ataatactcc ataaattttt cgagattaaa ctgagattca tcagtcagtt
    42061 cgttctcaat atagatattt aataaaatta gaatagctat tttatctgct tctgtttcaa
    42121 attttgagtg aaaagttgta gaagtatcgt acaaaactga atattcaaaa tgagaagcgg
    42181 tgaaatgcgc aagttcatga gataaatgaa aggcttctgc agtttcgcca tatagatttt
    42241 cattcaaaaa aatgattcta ggcttcggat aatagaagcc aggctctttc atttccatgt
    42301 aaactacttt caaattgtat tcgctcaaca tttctttcaa tttcaaatac atacaaccca
    42361 tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt
    42421 ctccttagat atttcttttc catcaaaaga aaaaacagta tcgttttctg ataaatccac
    42481 atgtttaggg gtttcttttt cttcgcgccc cagaagatag tctacagaga catcgaaata
    42541 gtcagcaatt tcttgcaaac gttctgtaga ggggtttgag ttcttaagtc tatataagac
    42601 gtttttacca tagcctaggt cttcttcaac tttatttaat gattttccct gttttttcgc
    42661 taactctttt attctctcaa acgttatcat atcaatattc ctttcgttca agaaaaaata
    42721 tttaacttta tttgttaaaa ttcgttgact attttaacaa aaggtgttaa tatcattctt
    42781 gtaaacaagt ttaacaacta aaaagacaac aaaaaacact attgattaat aaatgccaac
    42841 cgccaagaaa gcttttaaaa tcaatgttta tatgtcttat ttaactatgt tctgatttta
    42901 acactgtatg ttaatttagt caacgcagtt tttttaaaag ttgttaaatt tgtttacgaa
    42961 tataaaagaa aggagagaag aatatggaaa aaacagtctc ggcaaaaatc gaagatttga
    43021 aattagacat actgaaacaa gcaaaagtgg cgatggaaca cgcggtagat aaagaagact
    43081 ctgccatggt tgcagccata gcagagattt tagctcacgt ttagtcatcg tttttacggt
    43141 cttctggaag ctgaccatag ataacagcat aatgaatatc taaataagct tgagcaattt
    43201 cttttggcga aattgattcg cctttagtag atacttgtga ttcgtgattt agccatgcaa
    43261 caactaaatc cgcagcgatt tcatcagttt ttttattcat acttattcac caccttatgc
    43321 attatttcag tagaccactt actgataaga aaattatatc aaagaaagga atgaacaaaa
    43381 atgaacacac cacaaatttt tagttttgaa caacatgaag taagaacgtt tctagaaaat
    43441 gacattccgt atttcgtagc aaacgatgtt gccaaaactt tgggatacaa aaatcccagt
    43501 aaagccacca atgatcattg taaaaaatca attgaaacat ggggtaacga ttcgttaggt
    43561 cgtcgtcaaa aattcaaagt tattccagaa tcagatgttt accgcttgat tattaaatcg
    43621 aacttaccaa gcgctgaaaa atttgaggcc tgggtaatgg aagaagtcct tccaacaatc
    43681 agaaaaacag gtagctattc aaacgtacct caaagttttg cacaagcatt gcgtttagca
    43741 gcagatttag aagaaaagaa ccaattactc gaacaacaaa ttgccgagta cgaaccaaag
    43801 attagctact tagatacgat tctttcatcg acagatacgg tagctacttc tcaaattgca
    43861 gctgattacg gaatgtcggc aattgctcta aacaaattgc taaacgagtt aggtgttcaa
    43921 cataaagtta gcggacaatg gatactttac cgaaaacata tgaaccaagg atacacaaaa
    43981 tcgcacacaa gtgagattcc gaaagccgat ggcggcacta aagttgtaat gaataccaaa
    44041 tggacacaga aagggcgagt gtttatttac aacttattaa tcgcagaggg ctattaccct
    44101 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    44161 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcgaag ttcttaactt
    44221 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
    44281 gtaaaaagac ttctgcagaa gcaaatagaa tcatagattc gattattact atgtacgaat
    44341 tgtaaaggga ggaacagcta atgcaatatc tagaagcgaa aatcccagtt ccagaaggct
    44401 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    44461 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    44521 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgaaaaaaat aaaaatggat
    44581 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    44641 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    44701 agagcgctag tattcagcgc accgctaatc atctacgctt taggactttg gggaagcaga
    44761 caagcattaa tcggcacgat cgtttacatg gtttggattt ttatggggct tgatgaagct
    44821 gagtacagag caaaaaaagc cagtcgggag ggactgacta gatgagaaac aaaaaaatat
    44881 ctctaacgct attaactatc atcacgattg ttgtatcgca actattcttt ttaactatat
    44941 taaacgacat atcgatgtcg gaccgattgt tggttcaatc agctttattt ctgacctttg
    45001 aaaaaatggg taattacgaa agatagaatt tgagaaattg tttcttgggc aaataacgat
    45061 gttacccaaa ttacaagact aaaaattctt cctggaaaag ttgatagatt aatgccgaac
    45121 caactaagta tttcgctggg taagagaaaa aatttcttaa gagcaatcaa cggatttaaa
    45181 aaccagaatt ttcgataatg aatttcgttg tttttgtcaa aaatttgaga agcgataatt
    45241 ctaaaatttt cttcaagaat atcatctgat tgattcaagg aaagcgttac aaggctataa
    45301 ggcagaactt tgttaattct gggcatttcc ttaaccatta tttctctaat aggcgtcaca
    45361 ttattttgaa agtaaggatc agatttatag ctaccactat ggtcatttaa tcgtttgagg
    45421 tcatttatca ctttctcaat tgtagtaagt aaatagttaa tcgttgttat ttcagacact
    45481 gcattaacaa tttgcttaat ataaattatt gcataaacaa ctagaagcac atacaaaatg
    45541 tagttcattg aaaacaccac cattttttta cctaaattat accaaaagga gagaagaaaa
    45601 aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc
    45661 agaaagcttt gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga
    45721 tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat acggcagaaa
    45781 tcgacgagtt tttttcatta gtagagatgg ttttttcttg ttggctatgg gttttacagg
    45841 aaagaaagct atttacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt
    45901 tattcgcaag aatactgttc ctcaaacaat tgaagacatg atgatctacc aactagaaga
    45961 aatgaaagat gttaaaaaag atgtttccat gcttaaaaat actatgcgaa ttagcggaca
    46021 acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atggaagttc taggaggcaa
    46081 agaaagccga gcttatgaag aaatcagtaa aaaagtattc tcaaaatttt ggtctgaatt
    46141 taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaaaagat tcgatgatgc
    46201 tgtttcattt attgaaatgt ggttaccaga aactgcgatc cgcatggaaa tcgatcaact
    46261 gaacagacaa caaagacttt tcggtgatga aaatgaatag agctgaagcg ctaagaatcg
    46321 ggacggtaat tgctaatcgc tggtggagac acaataaacc aatcatccta agccaacaac
    46381 atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg actctgccag ccagcaaaga
    46441 gtcacataca aatttataaa actaggagaa tt
//
LOCUS       DAFBYI010000009        42930 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFBYI010000009 DAFBYI010000000
VERSION     DAFBYI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.009"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            147..431
                     /locus_tag="LLD04_000432"
     CDS             147..431
                     /locus_tag="LLD04_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK5647390.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(712..1131)
                     /locus_tag="LLD04_000433"
     CDS             complement(712..1131)
                     /locus_tag="LLD04_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK5647391.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1133..1717)
                     /locus_tag="LLD04_000434"
     CDS             complement(1133..1717)
                     /locus_tag="LLD04_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK5647392.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1764..3239)
                     /locus_tag="LLD04_000435"
     CDS             complement(1764..3239)
                     /locus_tag="LLD04_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK5647393.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3420..3731
                     /locus_tag="LLD04_000436"
     CDS             3420..3731
                     /locus_tag="LLD04_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647394.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3846..6182)
                     /locus_tag="LLD04_000437"
     CDS             complement(3846..6182)
                     /locus_tag="LLD04_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647395.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6324..6992
                     /locus_tag="LLD04_000438"
     CDS             6324..6992
                     /locus_tag="LLD04_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5647396.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6995..8038
                     /locus_tag="LLD04_000439"
     CDS             6995..8038
                     /locus_tag="LLD04_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5647397.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8275..9156)
                     /locus_tag="LLD04_000440"
     CDS             complement(8275..9156)
                     /locus_tag="LLD04_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5647398.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9315..10043
                     /locus_tag="LLD04_000441"
     CDS             9315..10043
                     /locus_tag="LLD04_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK5647399.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10199..10516
                     /locus_tag="LLD04_000442"
     CDS             10199..10516
                     /locus_tag="LLD04_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5647400.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10509..11117
                     /locus_tag="LLD04_000443"
     CDS             10509..11117
                     /locus_tag="LLD04_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK5647401.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11172..11963
                     /locus_tag="LLD04_000444"
     CDS             11172..11963
                     /locus_tag="LLD04_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK5647402.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12101..12589
                     /locus_tag="LLD04_000445"
     CDS             12101..12589
                     /locus_tag="LLD04_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK5647403.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12640..13512
                     /locus_tag="LLD04_000446"
     CDS             12640..13512
                     /locus_tag="LLD04_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5647404.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13688..14050
                     /locus_tag="LLD04_000447"
     CDS             13688..14050
                     /locus_tag="LLD04_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5647405.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14059..14811
                     /locus_tag="LLD04_000448"
     CDS             14059..14811
                     /locus_tag="LLD04_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647406.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14825..16627
                     /locus_tag="LLD04_000449"
     CDS             14825..16627
                     /locus_tag="LLD04_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647407.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16904..17945
                     /locus_tag="LLD04_000450"
                     /pseudo
     CDS             16904..17945
                     /locus_tag="LLD04_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(17997..20141)
                     /locus_tag="LLD04_000451"
     CDS             complement(17997..20141)
                     /locus_tag="LLD04_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5647408.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20498..21871)
                     /locus_tag="LLD04_000452"
     CDS             complement(20498..21871)
                     /locus_tag="LLD04_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5647409.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21871..22266)
                     /gene="rbsD"
                     /locus_tag="LLD04_000453"
     CDS             complement(21871..22266)
                     /gene="rbsD"
                     /locus_tag="LLD04_000453"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK5647410.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22294..23211)
                     /gene="rbsK"
                     /locus_tag="LLD04_000454"
     CDS             complement(22294..23211)
                     /gene="rbsK"
                     /locus_tag="LLD04_000454"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK5647411.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23319..24332)
                     /locus_tag="LLD04_000455"
     CDS             complement(23319..24332)
                     /locus_tag="LLD04_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5647412.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24413..25012)
                     /locus_tag="LLD04_000456"
     CDS             complement(24413..25012)
                     /locus_tag="LLD04_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5647413.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25198..25899
                     /locus_tag="LLD04_000457"
     CDS             25198..25899
                     /locus_tag="LLD04_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647414.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25909..28620
                     /locus_tag="LLD04_000458"
     CDS             25909..28620
                     /locus_tag="LLD04_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647415.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29071..30699
                     /gene="treC"
                     /locus_tag="LLD04_000459"
     CDS             29071..30699
                     /gene="treC"
                     /locus_tag="LLD04_000459"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK5647416.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30759..31940)
                     /locus_tag="LLD04_000460"
     CDS             complement(30759..31940)
                     /locus_tag="LLD04_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5647417.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32192..33715
                     /locus_tag="LLD04_000461"
     CDS             32192..33715
                     /locus_tag="LLD04_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5647418.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33784..34512)
                     /locus_tag="LLD04_000462"
     CDS             complement(33784..34512)
                     /locus_tag="LLD04_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5647419.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34525..35806)
                     /locus_tag="LLD04_000463"
                     /pseudo
     CDS             complement(34525..35806)
                     /locus_tag="LLD04_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(35947..36549)
                     /locus_tag="LLD04_000464"
     CDS             complement(35947..36549)
                     /locus_tag="LLD04_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5647420.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36794..37000
                     /locus_tag="LLD04_000465"
     CDS             36794..37000
                     /locus_tag="LLD04_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647421.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37312..38031
                     /locus_tag="LLD04_000466"
     CDS             37312..38031
                     /locus_tag="LLD04_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5647422.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38112..38795)
                     /locus_tag="LLD04_000467"
     CDS             complement(38112..38795)
                     /locus_tag="LLD04_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK5647423.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39013..41199
                     /locus_tag="LLD04_000468"
     CDS             39013..41199
                     /locus_tag="LLD04_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK5647424.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41545..41955
                     /gene="spx"
                     /locus_tag="LLD04_000469"
     CDS             41545..41955
                     /gene="spx"
                     /locus_tag="LLD04_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5647425.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            41969..42325
                     /locus_tag="LLD04_000470"
     CDS             41969..42325
                     /locus_tag="LLD04_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK5647426.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        14005 a         7549 c         8575 g        12801 t
ORIGIN      
        1 atgtttgttg actattgtga cttgtgaccg aggagttggc aggagaacac cgtttattcg
       61 aaaaaaggaa ctgcgacaag acttttgtca cagttccttc ttttttttgc tatgctatac
      121 tagatgtaaa aaataggaga aataaaatga gggaaattta ttcttttgaa tcaaaaatct
      181 atgcttccga agtcggcaag ggcggggcat atattatttt tccgtatgat ataagaaaag
      241 aattcggtgc tggaagagtt aaagtccaag caacttttga caatgtgcca tatgaaggca
      301 gtatcgtgaa tatgggcgtg aaaaatccag atggttcagt ctgctacatc cttggactac
      361 gaaaagatat tcgtaaaaac atcggaaaag aaatcggtga tattgtagca gttacagtga
      421 agcaaaaata aaagaggttg tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc
      481 aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac
      541 aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga taaacggcgt
      601 tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag ctatttttcc
      661 atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct ttcataatgg
      721 tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt tttccaacca
      781 atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga tttgtgcatt
      841 ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa cggcagtttt
      901 gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac catcgtggct
      961 agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg ttccgatatg
     1021 tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt taacagcagc
     1081 ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca tattaatcac
     1141 cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt ttcattgtat
     1201 ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga attggtggta
     1261 tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg gcatttaatg
     1321 gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca tcagcagcac
     1381 gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc atacttgccg
     1441 gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca cctaaatctt
     1501 tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag tcggattcca
     1561 gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc atcggatatt
     1621 cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa
     1681 tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct ttttatgatg
     1741 tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc tacttcttgg
     1801 aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt
     1861 tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa ttctgatggg
     1921 tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc ttggaaacga
     1981 gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc atcgactaaa
     2041 atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg acgctgaata
     2101 ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt accaccacct
     2161 gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac caaggcaaga
     2221 atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg accaatcgtt
     2281 gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac tacttgtaca
     2341 gctggattag catccccagt atagccagga aattctggca ttgcttttcc tgagttcgtg
     2401 ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat gaattcagca
     2461 cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg ttcgttacgc
     2521 aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc
     2581 gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat tggtttattc
     2641 aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat gatgtacata
     2701 ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct cattgttttt
     2761 tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc ttcaggagta
     2821 tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc tagatgaacg
     2881 acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg attttctaca
     2941 gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc acctacacgt
     3001 ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg
     3061 aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc accgatataa
     3121 cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc gtgatattga
     3181 cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg atctgtcatg
     3241 agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca
     3301 attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt gataatagaa
     3361 taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga ggaaggaaag
     3421 tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata gcgaatattt
     3481 tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg acagaagaga
     3541 taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac
     3601 tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga acagtattca
     3661 taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag aaaagtatac
     3721 cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg
     3781 tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat gtggttgtca
     3841 caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga
     3901 agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat cgcatggaca
     3961 ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct attgataggg
     4021 aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc taaaatacac
     4081 gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc ttttagtaca
     4141 ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat catgatcata
     4201 cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc atccatcaat
     4261 ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt atcttcatct
     4321 gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc ttcaaaattg
     4381 ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact aagcaatgtt
     4441 tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact gtctctttga
     4501 gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta
     4561 taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg taggatcccc
     4621 gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt attagggata
     4681 aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt gaatgttgta
     4741 ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc aagattcttc
     4801 aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc tgttgctgag
     4861 gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc tttgctagtg
     4921 tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat cgtactagat
     4981 aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg tactttgcca
     5041 tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt attacttaag
     5101 ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt
     5161 gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata atccgctttg
     5221 cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga aatagtaatc
     5281 ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa tccagaagaa
     5341 agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa
     5401 gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt ccaaaaactc
     5461 atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg aggatgggaa
     5521 tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg atgggccagt
     5581 tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg gatcaattcc
     5641 atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca caaaaggata
     5701 tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc accagataac
     5761 tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc atcttctgcc
     5821 cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc aacgttttca
     5881 ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt
     5941 cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt gattaccata
     6001 tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt tttaccagaa
     6061 ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga cacaccatcc
     6121 aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt taattgaagc
     6181 ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat cagaacaatg
     6241 atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa tctagaaatt
     6301 tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat gatccgcaat
     6361 tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc accgcattga
     6421 atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc attacagata
     6481 tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc accagatttg
     6541 acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa cgcgggttca
     6601 aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg atcctacgtg
     6661 tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata atcggacaaa
     6721 caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg attctgcctc
     6781 aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc ttcactcgcc
     6841 aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga acgatcgatg
     6901 tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa atcatcacag
     6961 tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat tttcacaatt
     7021 atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc tgtgttttct
     7081 cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc
     7141 aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta caggaatatc
     7201 gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa atatgagtct
     7261 ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag cagaagtaca
     7321 gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg agacactgag
     7381 gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa caatccaagg
     7441 atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa tatttttata
     7501 tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct taaaattgaa
     7561 caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag atgaacaaat
     7621 cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag aattggatat
     7681 cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa
     7741 tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata tcaaactatt
     7801 tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa tgaatgaaga
     7861 gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc aaatttctgg
     7921 gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg gacgtattga
     7981 ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta aacaatagga
     8041 aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta gtcgtttttt
     8101 tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct ttcttattga
     8161 actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa agagagcgaa
     8221 agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta gggcttagtt
     8281 ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct caccgacttt
     8341 acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca cagtattttc
     8401 ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt catttgcaat
     8461 catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt caaatccagc
     8521 ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa ggagcaaaaa
     8581 gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag gatgattttt
     8641 aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg aacttggtgg
     8701 ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt cattgtaagt
     8761 taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa
     8821 ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta agatatttaa
     8881 tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat ggtattgttc
     8941 tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt cgatttttct
     9001 attggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga
     9061 aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca ttgtttcggc
     9121 taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac ttattccaat
     9181 aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt
     9241 cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc gtagaaaaag
     9301 agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt ttgtttggcg
     9361 gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca cagctgacga
     9421 tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg atgaaaaata
     9481 tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt gttcatttag
     9541 gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa atcttcccta
     9601 gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg gtcattgttc
     9661 tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg acaggagaca
     9721 gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt tttgcgtgta
     9781 atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt tatattttgt
     9841 tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta gattttaaag
     9901 cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc
     9961 cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg atgtggacaa
    10021 attggattat gccactgtta taataaaaat caaagagaag ccattttttg ctgactaaaa
    10081 aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga aactatatta
    10141 tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag gtgacgtaat
    10201 gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat taagcaagga
    10261 agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg tcagtgaatc
    10321 tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga cctatgatga
    10381 accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt
    10441 agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt tattggagga
    10501 gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa attcctgaag
    10561 acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct ggaaaaacat
    10621 tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa ttaaaaatca
    10681 gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca aaaagacaaa
    10741 ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc attccactag
    10801 cactggggtt tatattggtt attgggggac tattggttgc tttactagcg ctcattttta
    10861 gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc atctcaggaa
    10921 tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc ggtctgttcg
    10981 ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa gggctttttt
    11041 atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa agagatctgc
    11101 attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt ttgattaccg
    11161 gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca ttaggcgctc
    11221 aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact gagacaaaaa
    11281 aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac
    11341 gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca gaaataaagg
    11401 agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt
    11461 tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa ttatctgaat
    11521 tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc aaaaaagtga
    11581 atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga acaataactg
    11641 cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac ttgatcgtca
    11701 caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa ggaaaagtca
    11761 tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat actatccaaa
    11821 cgtctggtaa atcttatatt gaaaaagact accagccaat aacaaatact ttgttagaac
    11881 aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt acgacggatc
    11941 aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga aaaaacagca
    12001 aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat agtaaaataa
    12061 gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag aagaaggtca
    12121 aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc aactaagcca
    12181 aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca attcagaagg
    12241 tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg tgaagaaaga
    12301 gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg atgggaagaa
    12361 ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag ggatggatct
    12421 agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa caaaattgga
    12481 ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca ctgaagacaa
    12541 agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat
    12601 tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa aagagcattt
    12661 tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt tggattgttt
    12721 ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag tatttttctt
    12781 tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa
    12841 gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt tttccttacg
    12901 atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt
    12961 ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt agtctttaaa
    13021 gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt tgtagaaatc
    13081 caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac attcaatgag
    13141 aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca aaaagagctg
    13201 caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc
    13261 tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc cattttgtct
    13321 gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt agagcaaatc
    13381 gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat caataattta
    13441 cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg cgacatgaaa
    13501 gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg gattattgtc
    13561 ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt
    13621 gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta agagagaagg
    13681 ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg tacttgcagg
    13741 ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac aaattacgac
    13801 agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca actatagata
    13861 tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca agggacaatt
    13921 gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt
    13981 cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat
    14041 taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac aaaagtatac
    14101 ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa
    14161 ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact tttgaatatt
    14221 ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa agatgtgacc
    14281 acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt tattttccaa
    14341 gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc tctttcttta
    14401 caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc
    14461 atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa acaacgggtc
    14521 gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga gcctacaggt
    14581 gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt gaatacaaaa
    14641 gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt
    14701 attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga tcaaagccgt
    14761 gattccttct atcgagaaat actatctatt cttggaaact tggaacagta gggggagatt
    14821 gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag actacttggt
    14881 gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat caatggcaag
    14941 caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca ttttccgatt
    15001 cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa attccttttt
    15061 gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa aaggcagaaa
    15121 aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta ctttagtggg
    15181 ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg atcagttaga
    15241 tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca ctgttttgtt
    15301 ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc
    15361 gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc ataaattctt
    15421 gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact atatgatgaa
    15481 acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga tcgtattagg
    15541 aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa agaaatcaga
    15601 acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact ttcgtattcg
    15661 tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac tgggcgcttt
    15721 gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca attctggata
    15781 tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag aactttcacc
    15841 aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact ataatgcaag
    15901 tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac cgccgaagat
    15961 cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag aggaacttcg
    16021 aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt
    16081 tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag acttccaagc
    16141 aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc catctttgca
    16201 aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg gtctattctc
    16261 tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc ttgctagctg
    16321 tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg aaatgcttga
    16381 aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt
    16441 attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac aaatgtttgg
    16501 tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat tcttcgtatt
    16561 gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat tgaaaaagag
    16621 attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagctcctca
    16681 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
    16741 gaaaaagagg ttgtgacaat atttttgtca caaccttttt ttcacggtct tttattagaa
    16801 tatagtaaat tttttctcgt tactcacgga ataagctgtt aagaaaacgg tttccatgta
    16861 tgctggaatc agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca
    16921 aaattttggc gaaaactatc acttgattac gatcacgaat caaaatggga catctttgtc
    16981 tgtcagtgat cttggggcac gggttgttca ttttcaaaca ataatcgata atgaaaaacg
    17041 agagttgatc ttaggttttg atgaagcaaa agaatatcta gaaaaagacc cttatatcgg
    17101 tgcttcaatc ggacgtatgg ctggacggat tacgaatggc acattcaaat tagatgatga
    17161 aacgttcgaa gtaaaaacag acccggacac tggtcattgg tttacatggt ggtgcaccta
    17221 gctttgaagc caagcagtgg cattatacag tcatcaatgg tgaaaacgaa gcatcggtca
    17281 ttttctatca gactagccca gatatggaaa acggatttcc tggaacttta gatgtagaag
    17341 tacggtatac gctgacgaac gatgatacat ggcgagtgac ggtccaaggg atcagtgacc
    17401 gcaaaacagt attcaatcct acaaatcatg tctatttcaa tttaaccggt gatccagcac
    17461 agtcaatcga cgaacatata ctttggttga atagccgatt atttgctacg ctcaataaag
    17521 atacaacgcc tacaggagaa gtagtttcag taagtggcac agcatttgac ttccaacgtc
    17581 ctaaaaaatt aggtgaggtc tttgccagtg attttcgcca atcaatgata gtgggcggca
    17641 tcgatcatcc atttttgttg aaaaaacaat ctggtttact aaatgctgcg gaattgacaa
    17701 gcccagatga aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc gtgattttta
    17761 cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa taaattagta aatcatggcg
    17821 gtatcacatt tgagactcaa gaactgccag gagcggagcg acttgaaagt tttggagatg
    17881 tcactctgga acctaaccaa gtacgcgagt ttgtaacgga atacaaaata aatataaaga
    17941 aatagaataa attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa tttattctat
    18001 ttctttaaag gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt tagtacagga
    18061 acagttgcag ctttaactgt acgagtagtt gtttctggta tctgtcttgc cgtaaaattg
    18121 ttccacaaat cccagattga agcgacacgg aagatggaag aagcgttgtt gacgcgtttt
    18181 atcgttgaaa attctaatcc tgtgattggc acgccatctg ttgtccattt gattggcaca
    18241 tcgaatcgct tagacatatt gaggatttca gcgtttgctt cttcagttgt tgctgcttct
    18301 tttttcggtt catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt caatttttgt
    18361 gaatattggc gatctgtagc ataacgtcct tccaaatatt tggttgcttc tttgtagtct
    18421 tttgtttccg acttccaagt tggtttgtag aaatcaggat tgccagtcag tccatttttg
    18481 atcaagtcgg catagtcttc caaactttcc ttgtaagaag gatatttacg gaattgtgcg
    18541 cggatttggt agctttgtcc ggaactatct tgttcaagtg tattgaaggt cacgctgctt
    18601 cctttatatg atcctttgat accaaaaagg ttatgatttg gttcgcttga taataagctg
    18661 tttccagatc cactttcaag gatagcttga gccatcatga ctgatgcgta gattccttct
    18721 ttttgcccga tttcctgtgc atcatctgca atctttttca agaactgatc agttgtttgg
    18781 ttttgactga aatggaacga gccatctaat ccatctaaaa tagatgagcc gcctgtcagc
    18841 gaacctgaag cagcaggtaa acctgctaag tttactcgga cagcaaaatc aggcgtgaaa
    18901 tctgaaatag aagttactcg aactttatct cctggttgag gcgcatggat aaattgtccg
    18961 ttcccaatat agatacctac atggtgagta acaccggaag aaggtcccca gaagacgagg
    19021 tcgccaggtt gggcctgact tactggaatc tgcgtacctg catattgctg ttcgcctgtc
    19081 cagccaccga tataatgacc ggttgctttc atatatacgt aataagtcaa accagaacag
    19141 tcaaaagtat ttggaccttt tgctccccat acatatggtt ttccaagatg cttgaatgct
    19201 tctcgaacga ttgcttgttg gacattcgtt gtatttgtag gcaatacagc atcaattggt
    19261 gattttacaa caaaatcaga agaagcagaa gatggattct ctataccgct ttgatttgct
    19321 gtatttggac gagatgaagc attgttcgtt ccgccattcg atgaaccatt gtttggatgg
    19381 tttggttctc tctgagtatt tccattgtta ttcgatccag atgggtttgt ttgattcgaa
    19441 gaattcccgc tgttcgaatt attgttatta ttgtttgaat tatttgaacc attattattg
    19501 ttgttattac ccacatctgg tttgtttggc gtttccggat ctggtgtact agggacttct
    19561 tcactaggat cagttggttt ctctgttgaa gtatccggtc cagctttaaa ttctgcagta
    19621 atagataaat tgttcgtacc tactgtaaag gtctttgcat cataatttac ttctttggca
    19681 ccattaacag cgactgaagc aatttgttca ccttcagctg gcgtaatagt gtaagttacc
    19741 ttcgtttccg ctaataaacc ttctaccgtt ccactttccg taatatccaa aggttgctgt
    19801 tcaggggaat ccgctgtcga agcagtcagt tggatcttac caatcaaaga aggagcaatt
    19861 gtaagggaat atgtctcggc tggcttttca ttttctgatg gatcagccgg atcaatttca
    19921 gttggctcgg tcgatcctgt tgttgagtta gttgttccag ttgggtcttc tgtaaaagaa
    19981 tccgttgttt cactagtagc tgagctggat gtagtcgtat ctgttgtgac tgtttgatca
    20041 gctatttgat tatctacggc aaatacggta ttggtcgaca aaacagacgg agcaatcagc
    20101 caagtagcag ctagcaaacg tatcgttttt ttatttttca taataaacta gtccttttcc
    20161 tttcattgat tttctgtctt attcagcatt cattagacat tttttggaac agataatcgt
    20221 gtcgaaaagc tttttctttt cataaatctg tctgtgtatt ctaaataaaa caatttgtaa
    20281 ataagcaacg agcattcatt cgtttttctt tgacgttcat tttattgcat agaccacctt
    20341 tgtttgggga taaacaagtg gggataaaaa tggttatgca tatgtaaatt ataccaaagt
    20401 ttggaaagta tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa gactgaaata
    20461 tgtgataaac atctcatact tcagtctttt tcattaacta ttcataagac attaggtctt
    20521 catcaaaccc gctaacgaga gtagtgatca ttgctacgac tagactaaca gccagtcctg
    20581 ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata
    20641 caaaagcgat cgctttcact tgggcaaatc ccattacagc tccaccagca gctgcaccaa
    20701 tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg
    20761 tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac
    20821 gtttacttcg cagaaataca cctaaagctg caccagccat tccaaaattg gctgctcctg
    20881 tcaacggaac gattcggtcg aacccataag cagagatatt actgatcatg acagggttta
    20941 cagcccattg gataccaaat attaccaaaa tattccaacc accagcgatg attgcgccac
    21001 tgatcggtcc gttaatatcc atcatccaag taattgcggc agctaaacct tcgccaaggt
    21061 aaacacccgc aggcccgaat accattaacg tcaaaggaac cgtgataact aatgaaagta
    21121 aaggtacaaa tactaattgc atattttcgc gaatatattt tttgaggaat ttttcaacaa
    21181 aagaataaaa gaaaatacct aaaatcgccg gcatgacagt agacgtatac tgcatcagta
    21241 ctacaggaat ccccagaaaa tcagtagctg ctttgccact gtctgaaagt aaagaggtca
    21301 gattcggttc aatcagtgct gcagcaatga cagccgcaat atatccattc actttaaacg
    21361 ttttggcagc agaaaaagca agaaagattg gcagaaaata aaagacggca ttcgacgcag
    21421 cagacaaaat atgataagtt ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa
    21481 caaccagacc ttttaatata ccagaaccgg caaacagcgg aatgatcggc gtaaagattt
    21541 ccgatatcaa accaaatagt ttttcgatcg gatttagctt ttctttcgga gctgaatgat
    21601 cgatttgacg cgtcaattcc ttgtaagcag gagccaatcc ctgatccatg attatttctg
    21661 catatctatc gagtaaaatg aaatacttaa tggaagggat ttgctgtttt aatttttctg
    21721 cattggctac tggctgtttt ttcaatttga tacgaagccg agtcgcacat ggaaggacag
    21781 aatcaatatt gtcttttcct cccaactgtt caatcagcat atgaatctgc tcggacagta
    21841 ccatctgttc aggattcatt gtcgtttcca ttagaagacc actccactca ctaatataat
    21901 attggcgtaa ggtgtcattt cgcctgtacg aataaatgca tgacagttat tcaactcttt
    21961 tttcatttct tcatgtggga taaagctaat cggtgtatct ggtaacaata atttgatttt
    22021 ttccaatatt tccgggttca ttgtctttat ttcttccgcc aattcaatcg attgtacctc
    22081 aagttcttct aaaacgtttt ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc
    22141 caaatctatt ttttcagtac caaaggggac aggcatgcct gcatccccga tacttaattt
    22201 atcaaagtgc cccatttgtg cgattactcg tgaaatatct gaattgatta cttttccttt
    22261 tttcatgttt gtttcttcct ctcattgtta attttagact gctgattgtt ctaaagcttc
    22321 gcggtaaggg atagaaggtt gcgcaccata agactgcaca gcaacagaag aggctagatt
    22381 tccataaagg atcgcttctt tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc
    22441 gccaataaat gtatcacctg cggctgttgt gtcaacggca tccactttaa aagcagggac
    22501 gataccgtgt tctttttctg tatgatagaa agcgccggca ctgcctagtg taatgattac
    22561 tgttccaata cctaattcat ggagtttttc ggctgctgcg accaaactgt tatgatctgt
    22621 cactctgatt cctgtaatga tttctgtttc tgtttcattt ggtataatga tatctgtctt
    22681 ttcaagtagt tcagtaggaa tcgtttcacg agctggtgca ggattcagga tcgttgtttt
    22741 tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca tctaagctac tttcaaactg
    22801 agcaataata aagtcactgt ttgtaatcag ttgcttgttt tttaggactt cttgttcatg
    22861 gaaagcatta tttgcccctg catggatcaa gatactattt tctcctgctg cgtctaccat
    22921 aatcattgct tgtccggttg tggcgccttg gatttcagct atcccagatg tatcgatgtt
    22981 ttcttttgtc agtagatcta ataactgttg tccttcgctg tcagccccaa cgcctccaat
    23041 aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct tggttggcac ctttacctcc
    23101 gccagcgtga aaaatctcat gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa
    23161 acgaagggta gtgtccatat taatacttcc aattactgta accgtgttca ttttacaaac
    23221 ctccaactat ctcatttatt ttgatgggaa aattattttt taaaacgttt tagtaaaacg
    23281 ttttaatagt tcgataaaaa tatagcacac cttgaaagct atttcaaggg tgcagttgaa
    23341 aatctttttt ctaatcgtac aggtaatctt ttttcttccc attctttttc tgggaattgg
    23401 atccgatcta acaataactt ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc
    23461 gttgtcagtt tcggcttgat atactcgcac atatctatat tatcatagcc aataatgctg
    23521 tagtcttcag ggatgctttt tccagcttcc tctaatccgc gatataatcc aaaagctaat
    23581 tcatcattta aagcaaacac accagttgca gcagaatcaa gaagttctgc tgtgatctgg
    23641 tagccacctt gttttgagaa ctgtgtaggt aagaggatca gttggtcgtg agggatttgg
    23701 taaacatcaa gagcagattt aaacccctca atccgagcgt ggacattttc cggtggattt
    23761 tcaggataga caagggcgat cgtttgatgt cctagactaa gcaagtgcat gcccgctagg
    23821 taaccaccgc gaaaatcgtc tgttcgtacg gcatcactaa atccttcaga ttttttctga
    23881 tccaaaacaa tgaagggccg gccttgtttt ttcaaatttt cgttgattgc atctgtagag
    23941 acagcagaag tcgcgataat gaatccatcc acacctcgac gtgtgagctc ggctagatat
    24001 tcgatttctt tttgatgatc agaatcagca ttacataata tcgtgacgaa attctgctta
    24061 tacaagatat cctcaattcc tcgcatcagt gtactgaaga atggattcgt gatatcagga
    24121 acgagtacac caattgtttt cgtttccttg gtaatcattt gtctagcaaa atagtcaggc
    24181 tgatagccta attcttcttt ggcagctaaa actttctgaa cagttttcgg actgaatttc
    24241 tccgtatttc catttaatat caaggagaca gttgtgattg aaacgccgga aagtttagcg
    24301 acttctttga ttgtgatctt ttttttcacc atgttcatac tcctatctaa atcctgtggt
    24361 tattttccca tgaaatcaaa aagaagtaaa tgaaaatcac atgtgccagt acttatttgc
    24421 tttgttggat agcttcgatt atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata
    24481 tcttttttat gaacaattga tgggattctt tgtcacgatc actgcaagca tcttcagcaa
    24541 gcacgatatg gtacccgtgt tgaaaggcat ctaatgcagt agcatataca ccgtttgttg
    24601 tcgctacacc gcttaaaatg atagtttcga ttccacgccg gcgaagttga agatcaagag
    24661 aagtaccaaa aaaagcactt ggattgtatt ttgtgatagt caacatatta tctgtatctt
    24721 tcaaagaatc cttcaaaagt aaatcagtga attctggagg tacagaagca gtcattccac
    24781 ccatatcagt ttgttgactc aaataaggga aagtggagat gtctacgttc actaaaactt
    24841 tcaaggcagc ggtacgtttc aatgttctta ctaacaaatc attgtttgtc agaatagatg
    24901 ctgcagtgtg tggagagagt gtaccgttat ttacgatctc tttttgcatg tcgatcgcga
    24961 caaaagcagt ttttgataaa tcaaaaatca tattttcctt taaatcattc atgatgttcc
    25021 ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta
    25081 aattaaagga ttcctttctt tagtctagag aaattcagtg gtaaaataaa taagtgtgag
    25141 aaaacgcatt cgatgcatag atatagcgag aaatatcgtg aaaatggagg aatcagagtg
    25201 aagtatgtcg aagtaaaaaa tgaatacaaa cgatacaaaa tgggagaaac gaccattaca
    25261 gcaaatgacg atatttcttt ttcaattgaa aaaggcgaat tagtgattat acttggtcca
    25321 agtggagcag gaaagtcaac gattcttaat attttaggtg ggatggatac acctgatgaa
    25381 ggtcagatca ttattgatga cacagatatt gcgcaatttt cagataaaca gcttactgct
    25441 taccgacgaa cagatgtagg gttcgttttc caattctaca atctcgttcc taatctaaca
    25501 gcaaaagaaa atgtggaatt ggcaacggaa gtttctcctg atgcgcttga tcctgtagag
    25561 gtattgaggc aagtgggcct agctcatcga ttgaataact ttccgtccca attatcagga
    25621 ggcgaacagc agagggtttc aattgctcgt gcactagcta aaaatcccaa acttctttta
    25681 tgtgatgaac cgacaggcgc gctggatttt gaaacgggca agcaagtatt gaaattattg
    25741 caaaacgcta gcagacaaca cggcaatacc gttttgatca ttacacataa ttctgcttta
    25801 gcgcccatcg ctgatagagt cattcacatt aatgatgcta aagtaaggtc tgttgaatta
    25861 aatgatcatc cttcatccat tgatgagatt gtatggtagg gggattttat gaaaacaaga
    25921 acatatctta aagcaggttt tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc
    25981 atccttattg tcatgctagg aacactttta tttgtaggtg tcaaaactgc tggacctgtc
    26041 atgcaaaaaa cgatggatca ctatgttggt acagcaggac tttctgattt gcaaatcgtt
    26101 tctactggtg gcttgacaca aaaagatatt gctcaagcag aaaagatccc agatgcaaaa
    26161 gtagaaacag gaaaacagat ctattattcc aatactggca aaaacgaagt gattcaaata
    26221 ttttcttata acaaaaataa caagcagaac aagctacaat taactgatgg aaaactcccg
    26281 gaaaaaccaa accagcttgt attggatgaa aaagcggaag atgaagggta taaaatcggt
    26341 gatatttatc agattgattc agatgaattg aaagaaaaag agtataccat cacgggcttt
    26401 gtccgttctc cattgttcat caacaatttg gagagaggat acgcgaacgt cggaaatggt
    26461 agcgtcgatt attttgtcta tttacctgaa tccgttttta aaacagatat ttattctgtc
    26521 ttatatcttg attttacaaa cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat
    26581 aaaatggagc aaaatcaaga aaaggcagaa aagtatttac aagatcgacc cgaagaacgc
    26641 ttagaagagc tgaaaaaatc ggccaacgaa gaattagaac cggcacagca aaaaatagcg
    26701 gatggcaaag cacaaacaac gcaagcaaga acacggttag aagatgctaa aaaacagtta
    26761 gcgcaacagt cagcaactat ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa
    26821 acattatctc aacaagaagc tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa
    26881 cgtgaactgg cctctgcaga ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta
    26941 actaaaccat catacctata caatgaacgt tcagaaaatg tcggttttca agaatttggt
    27001 gatctgtctg atcgtatcgc tgcgattgcc aatgtttttc ctgttttctt tttctttatt
    27061 gccgcattga tcacctttac gacaatgaca cgtatggtag aagaaaaccg cagggaaatc
    27121 gggacattaa aggcgttagg ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat
    27181 gcaagtctag cctcagggat aggtattgtt ttaggaacga tattaggaac gaatcttttg
    27241 ccaaggatca tttttgagct gtctaacgag cggtatgata ttgggagtgc gcttattttt
    27301 tatgactggc cgccgatttt acaagcagct gccgcattct tcattgccgc atttggcgct
    27361 gcaatgatcg ttttattcaa agatttaaga gaacggcctg ctgcattgct tcaaccgaaa
    27421 gcaccgaagc cagggaaacg catttttctt gaatacatca ctccgctgtg gtcacgcctt
    27481 agctttaatc aaaaaatcag ttaccgaaat ctcttccgat ataagtcaag aatgcttatg
    27541 gcgattatcg gaattgctgg atgtgccggg ctgatggttg caggcgtggg attgaaagat
    27601 tctctaagtt ctgtttcagc caaacaattt ggaacgatca ttgactatca agcaatcgtt
    27661 acattggatt catccacgga agatagtcgt caaaaagcag cggatatgct gcagaagcaa
    27721 gaaaaagtaa atgagatcct agctattcat actcaaaccg tagaattgag aaaaaaagga
    27781 catgcttcgc aaagtctgac agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt
    27841 atccatttaa ctgatcttga agagaaccca cgctccttgc ctaaaacagg aatcgctatt
    27901 acgcaaaaag tagcagaact atttgactta tctacaggag atcatatttc gctttatgat
    27961 gaagatcaaa aagaatggaa agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc
    28021 tttatttata tgtcccctga atattatgct tcagttactg atcaatcgat gatggataat
    28081 gcatttttgc tgcgtacaga taagatgaca acaaaagaag aagaaatgct ttccgagaaa
    28141 ctgcttgcca cagacgctgt gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa
    28201 gaagaatcaa tggcgaactt agactcggtt gtcttaatct ttgtcgtatt atcaggactg
    28261 ctggctttta tcgtcttgta taatctaact aatatcaata tttccgagcg tgtacgcgaa
    28321 ttatctacaa taaaagtcct tggatttttt gataaagaag tcacgatgta cattgttcga
    28381 gaaaatatca tctttactct tttaggaatc atcggaggat ttggtgtagg atatatattg
    28441 actgacttca tcttgcaaca agcgtctatg gagaatgtca tctttccatt agtgatcaca
    28501 tggtgggctt atgtcttatc agcaggatta actatcgtat ttacggttat tgtgatgatt
    28561 gttacccatt tcaaattaaa gcatatcgat atgattgatg cgttgaaatc gaatgaataa
    28621 aaacagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa cggcaaaata
    28681 gtttttcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccatg
    28741 aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca aataaaatta
    28801 gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt ttgaacacca
    28861 tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca aatacatgtc
    28921 tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat aaattttaca
    28981 aacgctttcg gttcagttat aataataaat gataacttgt atatataagt tgtggtaggt
    29041 aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt catttaccaa
    29101 atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt aaaaggaatc
    29161 aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct aaaccctatt
    29221 tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc catcgatcct
    29281 atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa ataccggata
    29341 gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa
    29401 gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga gccaacggat
    29461 tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg gaaatattat
    29521 ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc agtaagagag
    29581 gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt ccgtttcgat
    29641 gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa
    29701 cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa ccaagcaacg
    29761 tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac gatcgaaaat
    29821 tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa tttccatcat
    29881 ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga ttttgcagaa
    29941 ttaaagcgac tatttcatac atggggagaa aagatgagtg agggtaacgg ctggaatgct
    30001 ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagc tatcgatcag
    30061 tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg tgggacgccc
    30121 tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc gatttccgac
    30181 tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg attcacagaa
    30241 aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac acccatgcaa
    30301 tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa agttggaggc
    30361 aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat tttgtcctat
    30421 tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg agattatcat
    30481 gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga aggacagcag
    30541 ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc agaaggattc
    30601 acagaagcca atattcttat cgataattat tccgatactg ttcttcatca aagtctggag
    30661 ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt
    30721 tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata atcttcttca
    30781 gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta gcgttgtttg
    30841 aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg aataccaaaa
    30901 ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa gatcgtaatc
    30961 ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc gtggagaagt
    31021 tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc ttggaatgca
    31081 atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat acataacaac
    31141 ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa acaaacccag
    31201 tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga acccaattgt
    31261 tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg gacaatcgct
    31321 aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac aattggacca
    31381 gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg
    31441 ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc gtcaatagaa
    31501 gcgaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg tcggagactc
    31561 tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc ctgttcttca
    31621 agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat cacataaacg
    31681 ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa
    31741 agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat aatgattccg
    31801 acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc aagtaagata
    31861 actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa acgattcgtt
    31921 gtatctgttt cttttttcac tctttttcct cctttatcta taacttcatt atagacaaaa
    31981 gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat cattcatata
    32041 aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca tatgataaat
    32101 taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg gaacattcaa
    32161 atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta tattggccat
    32221 gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg aggacagtaa
    32281 agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa aagcaatcgt
    32341 cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc acgattatga
    32401 accttctgct aaagacatgg caaagatcca agatacagat atctttgttt ataatgatga
    32461 aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca atgtccatac
    32521 gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac atgatcatga
    32581 ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat ggctagctcc
    32641 aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag cttacccaga
    32701 aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc aagcactcca
    32761 tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc
    32821 tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg ctggtctttc
    32881 ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg ttaaagaaca
    32941 tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca gaacgttagc
    33001 aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga cgaatgaaca
    33061 aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag cgttgaaaaa
    33121 aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag aaaagagcgt
    33181 ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag attgggcagg
    33241 aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg tttttgaata
    33301 taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt atacgaaggg
    33361 ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat ttacgtttaa
    33421 gaatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct tgacctacga
    33481 ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt
    33541 tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac attttcattt
    33601 atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt ggccaacatt
    33661 ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc actgataggc
    33721 aaaaaagaac cgaagggttt ggatcggctg gtcatttagt cggtccaaac tattcggttc
    33781 tttttatgaa atcaatttct ttaatccacg gatagaggct gggatggtct cgccgtttgc
    33841 caacgtgact tctctggttt ttttattaat cgaccgcaca ttttgtgtat tgataacata
    33901 ggatttatga caacggataa aattccgatg cattttctcg atttctttga tcttctcata
    33961 gaattgtaat tgggcatgag tcaaatggac ttccagttta tgtggcgctc ctagcgaagt
    34021 agaaaaatat tggatgtctt tgatcggtaa aaatttgatc tcatcattaa actctatttt
    34081 gaagatatct ttttggattt gggacatttg aaaatcttcg aaaattttgc tcaggatatg
    34141 ctcgacattt tcttttaaat cataaagatc ttctttgacg atataatcag tcggttcgat
    34201 gttgcttttt aagattttca tcgacatttc cgtatgggaa gtcacataga ttatttttcc
    34261 taaaggatct aattcacgga tcaatttacc gatcgctaca ccatccattt cactgtcttc
    34321 tagttcgata tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg ctgcattgag
    34381 aagttcttcc ggatcagagg tagaaatatg gacataactg tctaagtttt caatcatgat
    34441 tctatttttg atcaccgttg ataaaacttc taattgtttt ggatcgtctt cacaaatata
    34501 aatgggaatc atatcattac tcctttattt tatttgtata taaatcgttt gagaaaaacg
    34561 ctggttttca attgatgttt caaggaaaag ataaggattg gtctttgtca attcatccaa
    34621 gataaacaag ccaaggcctt cgttttttgg atttgttttt gttgagaacc ctttttgttt
    34681 caactggttt aacggtaatg tatttgttgt tgtattgatc acacgaatca tcacggaatg
    34741 attcttcttg agtatggtca attcgatttt tttcttcttt gcagcactag ctccttcaat
    34801 cgcattgtcc aagataatac caagcatacg tacaaggttt accgtatgtt tcgtatccag
    34861 ttcgattggc tcaggaaaat gaagctggac gatcaaccct ttttcctgtg ccatcatgag
    34921 ttttaatgac agtaaactac ggatctcagg aactttcata ttatccaaca tacttagatt
    34981 ggccggaaaa aggttcatca tctggcgtgt aggtaatatc gtttgatcat aataagaccg
    35041 taatccttct aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca gattttggta
    35101 atcatgtttg aattctctca agtcttgata gtttttttct agatctttga tataatcctg
    35161 ttgcatcctg acttgttgct ctaataactg atcagctaat ttcttattag agattttcca
    35221 ataataaacc aagatgatca ggccaacaca cagatataga atgaaaaaac tcgtattcaa
    35281 agctaagaca tcaggtgttt caccagaaaa acgggtataa aaaataatga tatagtatgt
    35341 aaataatatc aatgtcccga tcgtaccgat catacggcgg tccacagtgt gaaaccaagt
    35401 ctctaacgat ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg ataagacaat
    35461 gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc
    35521 gccgatgctt gttaagtgat ctcctaaaag cgacgtcaat aacccaaaaa gaaagcgcgt
    35581 aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag
    35641 caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa
    35701 tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac
    35761 aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc
    35821 attgattttt accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt
    35881 ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt
    35941 aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt
    36001 aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta
    36061 agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt
    36121 taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata
    36181 gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt
    36241 caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag
    36301 acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga
    36361 aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac
    36421 cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac
    36481 catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt
    36541 atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc
    36601 ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt
    36661 cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg
    36721 acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga
    36781 tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa
    36841 atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt
    36901 gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg
    36961 gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga
    37021 gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa
    37081 cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt
    37141 ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga
    37201 gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt
    37261 atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata
    37321 attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt
    37381 gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg
    37441 ctattttatt ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa
    37501 cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc
    37561 tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa
    37621 gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca
    37681 caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga
    37741 atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac
    37801 tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa
    37861 atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc
    37921 gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc
    37981 cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac
    38041 taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg
    38101 attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc
    38161 tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac
    38221 agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga
    38281 tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc
    38341 gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt
    38401 ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg
    38461 ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga
    38521 acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt
    38581 agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat
    38641 tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa
    38701 gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt
    38761 cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat
    38821 ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta
    38881 aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag
    38941 acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag
    39001 gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg
    39061 gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc
    39121 tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa
    39181 agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat
    39241 caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga
    39301 gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa
    39361 caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta
    39421 aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat
    39481 agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa
    39541 ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag
    39601 ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct
    39661 cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa
    39721 gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg actgtatgga
    39781 ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta
    39841 ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa
    39901 tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg
    39961 aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt
    40021 atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct
    40081 accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca
    40141 aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa
    40201 aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg
    40261 ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata
    40321 ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt
    40381 ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa
    40441 catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag
    40501 gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc
    40561 gatcaatact tttctcctaa atatcaaaat atatccgttg cagattatat tttgtcgata
    40621 agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa
    40681 acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata
    40741 gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct
    40801 actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa
    40861 acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac
    40921 aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg
    40981 ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca
    41041 gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat
    41101 cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt
    41161 atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga
    41221 agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga
    41281 aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg
    41341 tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat actattatca
    41401 aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag ttattttgat
    41461 tttttttata ttttattagc taataattgg tattttaaaa gaaattactg tagaattgga
    41521 ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc
    41581 tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt
    41641 tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga
    41701 gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac
    41761 gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga
    41821 cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca
    41881 tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac
    41941 atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg
    42001 tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt
    42061 tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga
    42121 agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga
    42181 cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct
    42241 cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat
    42301 ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc
    42361 atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt
    42421 tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg
    42481 atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt
    42541 tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg
    42601 ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat
    42661 tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag ttatgataaa
    42721 tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa
    42781 ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa
    42841 cactcctgat gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg
    42901 gcgtcttgat tcaattcaag acaacaaaca
//
LOCUS       DAFBYI010000010        41209 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFBYI010000010 DAFBYI010000000
VERSION     DAFBYI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.010"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(26..328)
                     /locus_tag="LLD04_000471"
     CDS             complement(26..328)
                     /locus_tag="LLD04_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647427.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="LLD04_000472"
     CDS             complement(353..2446)
                     /locus_tag="LLD04_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5647428.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="LLD04_000473"
     CDS             complement(2935..3699)
                     /locus_tag="LLD04_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK5647429.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LLD04_000474"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LLD04_000474"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK5647430.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="LLD04_000475"
     CDS             complement(4704..5780)
                     /locus_tag="LLD04_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK5647431.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="LLD04_000476"
     CDS             complement(5804..6397)
                     /locus_tag="LLD04_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK5647432.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LLD04_000477"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LLD04_000477"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK5647433.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="LLD04_000478"
     CDS             complement(8067..8366)
                     /locus_tag="LLD04_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK5647434.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LLD04_000479"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LLD04_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK5647435.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="LLD04_000480"
     CDS             complement(10121..11032)
                     /locus_tag="LLD04_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5647436.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="LLD04_000481"
     CDS             11152..12009
                     /locus_tag="LLD04_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5647437.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="LLD04_000482"
     CDS             complement(12196..12558)
                     /locus_tag="LLD04_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK5647438.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="LLD04_000483"
     CDS             12904..14343
                     /locus_tag="LLD04_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647439.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="LLD04_000484"
     CDS             complement(14350..16902)
                     /locus_tag="LLD04_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK5647440.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="LLD04_000485"
     CDS             complement(17016..18347)
                     /locus_tag="LLD04_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647441.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="LLD04_000486"
     CDS             complement(18313..19791)
                     /locus_tag="LLD04_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647442.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LLD04_000487"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LLD04_000487"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5647443.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="LLD04_000488"
     CDS             22401..23828
                     /locus_tag="LLD04_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647444.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="LLD04_000489"
     CDS             complement(23920..24285)
                     /locus_tag="LLD04_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5647445.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="LLD04_000490"
     CDS             complement(24282..24647)
                     /locus_tag="LLD04_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5647446.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="LLD04_000491"
     CDS             complement(24839..25087)
                     /locus_tag="LLD04_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647447.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="LLD04_000492"
     CDS             25086..25214
                     /locus_tag="LLD04_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK5647448.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="LLD04_000493"
     CDS             25271..26965
                     /locus_tag="LLD04_000493"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK5647449.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="LLD04_000494"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="LLD04_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27739..28872
                     /locus_tag="LLD04_000495"
     CDS             27739..28872
                     /locus_tag="LLD04_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK5647450.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEVLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="LLD04_000496"
     CDS             28903..29223
                     /locus_tag="LLD04_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK5647451.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="LLD04_000497"
     CDS             complement(29252..29716)
                     /locus_tag="LLD04_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647452.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="LLD04_000498"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="LLD04_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="LLD04_000499"
     CDS             complement(31133..31450)
                     /locus_tag="LLD04_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5647453.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="LLD04_000500"
     CDS             complement(31476..31796)
                     /locus_tag="LLD04_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647454.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="LLD04_000501"
     CDS             complement(32260..33675)
                     /locus_tag="LLD04_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5647455.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="LLD04_000502"
     CDS             complement(33692..35008)
                     /locus_tag="LLD04_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5647456.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="LLD04_000503"
     CDS             complement(35158..35856)
                     /locus_tag="LLD04_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK5647457.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37101)
                     /locus_tag="LLD04_000504"
     CDS             complement(36145..37101)
                     /locus_tag="LLD04_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5647458.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LLD04_000505"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LLD04_000505"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5647459.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="LLD04_000506"
     CDS             complement(38222..39856)
                     /locus_tag="LLD04_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647460.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="LLD04_000507"
     CDS             complement(39843..41177)
                     /locus_tag="LLD04_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647461.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12505 a         8475 c         6957 g        13272 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccaactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aagtactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DAFBYI010000011        38388 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFBYI010000011 DAFBYI010000000
VERSION     DAFBYI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.011"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(200..1591)
                     /locus_tag="LLD04_000508"
     CDS             complement(200..1591)
                     /locus_tag="LLD04_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK5647462.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1804..2190)
                     /locus_tag="LLD04_000509"
     CDS             complement(1804..2190)
                     /locus_tag="LLD04_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK5647463.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2246..2869)
                     /locus_tag="LLD04_000510"
     CDS             complement(2246..2869)
                     /locus_tag="LLD04_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK5647464.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2933..3601)
                     /locus_tag="LLD04_000511"
     CDS             complement(2933..3601)
                     /locus_tag="LLD04_000511"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5647465.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3613..4053)
                     /locus_tag="LLD04_000512"
     CDS             complement(3613..4053)
                     /locus_tag="LLD04_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647466.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4112..4783)
                     /locus_tag="LLD04_000513"
     CDS             complement(4112..4783)
                     /locus_tag="LLD04_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5647467.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4968..5366)
                     /locus_tag="LLD04_000514"
     CDS             complement(4968..5366)
                     /locus_tag="LLD04_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK5647468.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5363..6136)
                     /locus_tag="LLD04_000515"
     CDS             complement(5363..6136)
                     /locus_tag="LLD04_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5647469.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6133..6816)
                     /locus_tag="LLD04_000516"
     CDS             complement(6133..6816)
                     /locus_tag="LLD04_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5647470.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6983..7870)
                     /locus_tag="LLD04_000517"
     CDS             complement(6983..7870)
                     /locus_tag="LLD04_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5647471.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7867..8736)
                     /locus_tag="LLD04_000518"
     CDS             complement(7867..8736)
                     /locus_tag="LLD04_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647472.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8873..10159)
                     /locus_tag="LLD04_000519"
     CDS             complement(8873..10159)
                     /locus_tag="LLD04_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5647473.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10432..11010
                     /locus_tag="LLD04_000520"
     CDS             10432..11010
                     /locus_tag="LLD04_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5647474.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11010..11939
                     /locus_tag="LLD04_000521"
     CDS             11010..11939
                     /locus_tag="LLD04_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5647475.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11936..13672
                     /locus_tag="LLD04_000522"
     CDS             11936..13672
                     /locus_tag="LLD04_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5647476.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13735..14571)
                     /gene="pheA"
                     /locus_tag="LLD04_000523"
     CDS             complement(13735..14571)
                     /gene="pheA"
                     /locus_tag="LLD04_000523"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK5647477.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14568..15077)
                     /locus_tag="LLD04_000524"
     CDS             complement(14568..15077)
                     /locus_tag="LLD04_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK5647478.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15070..16365)
                     /gene="aroA"
                     /locus_tag="LLD04_000525"
     CDS             complement(15070..16365)
                     /gene="aroA"
                     /locus_tag="LLD04_000525"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5647479.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16378..17469)
                     /locus_tag="LLD04_000526"
     CDS             complement(16378..17469)
                     /locus_tag="LLD04_000526"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK5647480.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17486..18652)
                     /gene="aroC"
                     /locus_tag="LLD04_000527"
     CDS             complement(17486..18652)
                     /gene="aroC"
                     /locus_tag="LLD04_000527"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK5647481.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18654..19718)
                     /gene="aroB"
                     /locus_tag="LLD04_000528"
     CDS             complement(18654..19718)
                     /gene="aroB"
                     /locus_tag="LLD04_000528"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK5647482.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19751..20773)
                     /gene="aroF"
                     /locus_tag="LLD04_000529"
     CDS             complement(19751..20773)
                     /gene="aroF"
                     /locus_tag="LLD04_000529"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK5647483.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21432..22406)
                     /locus_tag="LLD04_000530"
     CDS             complement(21432..22406)
                     /locus_tag="LLD04_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK5647484.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22387..24270)
                     /locus_tag="LLD04_000531"
     CDS             complement(22387..24270)
                     /locus_tag="LLD04_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK5647485.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24288..26513)
                     /locus_tag="LLD04_000532"
     CDS             complement(24288..26513)
                     /locus_tag="LLD04_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK5647486.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26628..27662
                     /locus_tag="LLD04_000533"
     CDS             26628..27662
                     /locus_tag="LLD04_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647487.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27729..28742)
                     /locus_tag="LLD04_000534"
     CDS             complement(27729..28742)
                     /locus_tag="LLD04_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5647488.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28820..29008)
                     /locus_tag="LLD04_000535"
     CDS             complement(28820..29008)
                     /locus_tag="LLD04_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK5647489.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29448..29576)
                     /locus_tag="LLD04_000536"
     CDS             complement(29448..29576)
                     /locus_tag="LLD04_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5647490.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29579..30952)
                     /gene="rlmD"
                     /locus_tag="LLD04_000537"
     CDS             complement(29579..30952)
                     /gene="rlmD"
                     /locus_tag="LLD04_000537"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5647491.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31063..32118)
                     /locus_tag="LLD04_000538"
     CDS             complement(31063..32118)
                     /locus_tag="LLD04_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5647492.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32128..33558)
                     /gene="gatB"
                     /locus_tag="LLD04_000539"
     CDS             complement(32128..33558)
                     /gene="gatB"
                     /locus_tag="LLD04_000539"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK5647493.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33555..35027)
                     /gene="gatA"
                     /locus_tag="LLD04_000540"
     CDS             complement(33555..35027)
                     /gene="gatA"
                     /locus_tag="LLD04_000540"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK5647494.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35027..35332)
                     /gene="gatC"
                     /locus_tag="LLD04_000541"
     CDS             complement(35027..35332)
                     /gene="gatC"
                     /locus_tag="LLD04_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK5647495.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35438..37474)
                     /gene="ligA"
                     /locus_tag="LLD04_000542"
     CDS             complement(35438..37474)
                     /gene="ligA"
                     /locus_tag="LLD04_000542"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK5647496.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37491..>38388)
                     /locus_tag="LLD04_000543"
     CDS             complement(37491..>38388)
                     /locus_tag="LLD04_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBK5647497.1"
                     /translation="VDLANIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRS
                     GYLEDLKIQNTLEAQARIENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLA
                     LVSDIDNLEEDASQVTLMTLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERR
                     LAYVGITRAEEALYLTNAFSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPV
                     FAKKTAYSYKQPETAVVPSKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLEL
                     DVAFPSQGVKRLLAAFAPIEKA"
BASE COUNT        10702 a         8381 c         6801 g        12504 t
ORIGIN      
        1 taaaatatga ggaactattt ttgtttggct gttcttatta cttgatgcag ggcagctttt
       61 tcacaacctc ttattcacgg tgtttcagaa gtagcttctc ggaaataagc cgaaatttca
      121 caaaaatttg agaaactatt tttaggaaac ttcttcttat ttcttaaagc tgaccacttc
      181 tatcacaacc tctttttgac tatgcattca catcgatcac tttagaatca tcattgatga
      241 catcgcgatc taaatctttg atgcgtgcac ttgtcacgat accagctacc atgctaccgc
      301 tgacgtttac tgctgtacgt gccatatcga ttaatggttc aacagagata actaacccaa
      361 cgattgctac tggcaagttc attgccccta aaacgatcag tgaagcaaat gttgcgcccc
      421 caccgactcc agctacacca aaagaactga ttgttacaat tgccacaagc atcaaaataa
      481 attccaaact aaagacattc actccagctg ctggtgcgac gatcgttgcc aacatagctg
      541 gataaacgcc agcacagccg ttttgtccaa tagacaaacc aaaacttgct gagaaattag
      601 ctgttgcatc atctactcct aaagcttttg tctgagtctc gatattcaat ggcaaagctc
      661 cagcacttga acgtgaagta aacgcaaagc tcaataccgg aaaggctttt ttgaaatagt
      721 tgattggatt gacttttgca ccaatcaaaa tcagtgagtg gacaatcaaa acagcaagca
      781 atgctacgta agaagcaacg acaaatttac ctaaattgac gatagctgca aaatcacttg
      841 ttgccaaaac gtttgtcatc aaagcaaata ctccataagg tgtcaaacgc aaaacaagcg
      901 tcacgatccg cataatgatt ttatacagac tgtcgactaa atctgaaaag aatgctgctt
      961 cttttggcgc ttttcttttc acgcctagat aagcgattcc gacaaatgcc gcgaaaataa
     1021 cgactccaat cgtacttgtt gcacgactac ctgctaaatc agcaaaaacg tttttaggga
     1081 taaaggataa aatctgctgt ggaatcgata agttttcaac tgtttcctga cgtgttgcca
     1141 gttcagagat tcgtgctgtt tctgcagctc cttgtgtgaa tttagctcct tggagtccga
     1201 acagcattgt tgaaccaatt ccgatcaatg ctgcaatagc tgttgttcca agtaaagaag
     1261 tcaatacagt cgcactaatt ttccctagct tttcagaaac tttcattttt gtgaatgcac
     1321 caatgatcga aataaacact aaaggcataa ctagcatttg caagaatgat acatatcctt
     1381 gacctacaat cccgatccat tccattgatt gagtaacgac tttgtcgtct acaccaaaga
     1441 tcaattgaag cagaccgcca aagacgatac caactcctaa tgctgtaaag acgcgtgttg
     1501 aaaattttgt atggcgcttt tgcatacgat agaacacata taataatgcc gcaaaaacta
     1561 gaaccactaa aactgtgatg agtgttgtca tttttctcct cctcatatta ttaatgacaa
     1621 gtccatcgaa ggaagtaaag gttcccgaga atgattcttc aaccatcctc ctgaaaaaat
     1681 ttttttcagt gacttttata ttttagacga tttttatcga aatgacattc tttttgctta
     1741 caattagtaa taaaaaaaca tacctgtgat agctttaatc tatcgcaggt atgtttttac
     1801 tgtctattct tgtttttctg attgaaaatt caattttacc ccaggctgat ctaaggcgat
     1861 ctctgtcacc tcataagaca ttcggcgtac gatatcgaat aacggtgcta ctagctcgtc
     1921 cacttcttct tgtgtgacat cattttgacc ttcaattttc cgattgagga tatgattgat
     1981 ctgactcaca cttccagata aaacatatgt atcaaacact aaacggaact ctaagcgtgc
     2041 tcctacgatg ctatgttctt tcggatagtt ttcgatcgtt tcttttaaag gcgagaagcc
     2101 tactttcaaa tctgtttcta cttcttggtt cggcaatctc atatcataat gaaatgcttc
     2161 tacaacttct ttttgtcttc tgatttccat ataattttat cactccttta tgaaaattat
     2221 aacatatctc ctttgaaagg ggatactact ttttaatgat gctctgcaag gtaaggattt
     2281 cattcagatt tcctaccaca taatctgccg ggatctcacc cagaaaatga tcgatgtcgt
     2341 aaagacaagt agcgactcct gcatttttcc ctgcttcgat atctaaacga cgatcgccga
     2401 tcatcatcgt gtctgttctt tctaaatgaa aagtatcgat caaataattt aaagatgctg
     2461 gatcaggttt acgaggaaag tcctgatcga tccctactac ttcctcaatc agatgtgcca
     2521 atcgatgttc ttttaacagg ccccatgttg attctgtcaa acggtgggtc aggatgaaat
     2581 gacgaccgcc gttgtctttt agcatttcca gtacttgttt cgtttctgga aatggacggg
     2641 aaacttccgt actttctttt tcatatcggt gaaataattc attaaactct ctggcattca
     2701 agtttgattc attgatcaaa taactagtag aatacttttt cattttgaag tagatttctt
     2761 ttttgtccat actgatacca aaatctttta gtgcttgcca tgcaccatct accattgcgg
     2821 gatacgtatc aaacaatgta ccatcaaagt cccatatata atttttcatg ttccttttgt
     2881 tccattcctt tgcttctttt ttctatctct cacttttaga taaaggtgtt ctttaaagta
     2941 tttttttgtc cagaattgtc aaatcatctg ctaatgtata ctggattggt tgtgcatttg
     3001 ggatatcgat cgtatccatt tgatcttcag gaacatcttc taaatatttt gtcagtgctc
     3061 ggatgctatt accatgaccc acaactagta aatcttgccg atcttttagt tttggtgcta
     3121 gatgatcttg ccataaggga atgatccgat tcactgtcat ttccagactt tctccgtatg
     3181 ggatcaaatg cggatccaag tgatcatatc tgcggtcaaa tctattttct gcggttaacg
     3241 gcggaacttc cgtatatcct ctgcgccatg tttttacttg atcttttccg tatatttctt
     3301 tcatcttatc tttgttttta cccacaagat ccccataatg ccgttcattt agacgccatg
     3361 tttttattat aggaagccat aactgaccca tttcttctag tgcgacttgt ccggtaagaa
     3421 tcgcacgttt caaaacagag gtataaacgg catcgaattg aagtccggct tcaagcattt
     3481 tttgaccggc aactgctgct tgttcttttc ctttttctgt taaggcgaca tctagccatc
     3541 ctgtccagta attttcaaaa tttgcttcgc tttcgccatg acgtaataat actaatttca
     3601 ttcttttctc ccttaaacat tcgtttcgaa ttttatacca catgttttgc atgtcacacg
     3661 tattcgtcct tttcctttag gtgcacgctg cttttgatga caattcgggc actcaaaaaa
     3721 agtataactt tttcgatcca ttttgacttg ctttttgttt tttatctgat gatatttttc
     3781 tttgactcta tcaagatatt tcaaataacg ttgattctca tttagtctgg ggtagatttt
     3841 ttttgatagg aaacgaaata tcatccatcc gaaaaagacg atgaacaaca attgtccgat
     3901 tctatagggc aaccaacgac ttagcaaact aaagacaagc gcagcgatca atagaccgcg
     3961 attcaactga tcaaatttcg catatcttcc ctgcataagt ttgtgatagt tctgctgaaa
     4021 tcgtattagc ctttctaacc atgtttgcat catattatct cctccatcat ctaatattta
     4081 ttatgacaaa aaaatccaaa aagaaaaagc cttaattggc cttttccatt gcaacttggt
     4141 gaaggacatt ccgatttctg gtaaagtcaa tcacaatcac tttcttttta tcttctctgt
     4201 tgactactcg aaatgtgtcc gtcaatggaa gaaacagctt cttagatggt agctgttgca
     4261 tacgtataaa acgatggatc atcgtaatca attgaggaaa ttgaatagac gtgtgaagca
     4321 gacctggata ttttttcaac agctgtgtcg ttttgatcag attccgtttt ttactgatca
     4381 cttttaaaaa gctttttaga atcttttgct gacgtctaaa aatagaaaaa gaattttcgt
     4441 catctatttg ataacgcatt tgtttttgca gttctgctgc agtcagatgg atcttcccat
     4501 cgtttgtatc gatcattaaa ccttctggaa acagttcaac cactgctcga tcccaagatt
     4561 caagagaaag ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca
     4621 aaacagccga cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga
     4681 actctggtga aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag
     4741 atcgctcaat cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt
     4801 catcattttt aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat
     4861 aaaaatttac tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac
     4921 gctcatccgc cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac
     4981 ttttctctat cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga
     5041 tgaatcttag tgataccaat gactttgtct acatcaatac taaaacaaat accattacct
     5101 ggaatatcga tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg
     5161 ggtactaatg tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct
     5221 gaatcatgta caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct
     5281 tttgaaaagt cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga
     5341 agattcaatg ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt
     5401 cgattctgtc tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata
     5461 ttggtgtcaa tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt
     5521 caatttgtgc agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg
     5581 ttgctactcc accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta
     5641 aaataaatgc tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca
     5701 aacgccacat agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga
     5761 ttttcgatcg gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg
     5821 ccggctcagc aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat
     5881 tgtaaggcaa tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat
     5941 tgactccgtg caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt
     6001 caaagaaacg ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta
     6061 cgataggtgc cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt
     6121 gctgccagaa actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa
     6181 cgaagcaata ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact
     6241 agtcatccct ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc
     6301 ccccgaatca aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac
     6361 gcctatgtag cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag
     6421 cgcaaaggct agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc
     6481 aatctctccg tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc
     6541 aatcgtgatc acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt
     6601 tttgatcata tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa
     6661 caaaaagagc gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc
     6721 ttctccttct aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa
     6781 tatagcagca atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa
     6841 gtttacctta aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt
     6901 ctcaacataa aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa
     6961 ggaacgcgct tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct
     7021 tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga
     7081 tcattccata gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc
     7141 gtttatcagg agattgcaaa gctactaagg gccataaata attgttccag ctactcatga
     7201 atgtgatgat tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa
     7261 aaatacttag ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag
     7321 atttaacatt ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga
     7381 caaccaagaa actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata
     7441 aagggatcat caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta
     7501 ggtcttttcg tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca
     7561 atgcaaacac agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat
     7621 tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt
     7681 ttcccgatgt gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga
     7741 aaatggaaat aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg
     7801 aacgaagttc taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca
     7861 tatttttcat cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa
     7921 ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact
     7981 caaattatag atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat
     8041 attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa
     8101 aatgattggt tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc
     8161 gtcaatagat gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa
     8221 aatcatattg tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca
     8281 aattggatga gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt
     8341 gtttacaagg ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga
     8401 agtgatacat ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc
     8461 atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa
     8521 atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata
     8581 attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa
     8641 agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa
     8701 agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt
     8761 gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg
     8821 tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag
     8881 ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat
     8941 tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt
     9001 aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac
     9061 tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata
     9121 ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt
     9181 ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc
     9241 cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa
     9301 tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag
     9361 ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg
     9421 tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag
     9481 atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct
     9541 gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc
     9601 ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca
     9661 tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta
     9721 cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat
     9781 acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag
     9841 aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg
     9901 tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa
     9961 caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga
    10021 tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac
    10081 tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa
    10141 ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg
    10201 ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat
    10261 ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc
    10321 tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc
    10381 tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata
    10441 cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat
    10501 tggtctgagc acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat
    10561 ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat
    10621 ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc
    10681 tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat
    10741 cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa
    10801 aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag
    10861 aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa
    10921 cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta
    10981 aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc
    11041 ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac
    11101 ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt
    11161 ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca
    11221 aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt
    11281 tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact
    11341 acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg
    11401 atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg
    11461 ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat
    11521 tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac
    11581 tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg
    11641 agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc
    11701 atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa
    11761 aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta
    11821 ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt
    11881 ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag
    11941 aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct
    12001 ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat
    12061 ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag
    12121 tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag
    12181 ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca
    12241 aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt
    12301 tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc
    12361 tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct
    12421 ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa
    12481 aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa
    12541 gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat
    12601 gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat
    12661 gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg
    12721 tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc
    12781 taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc
    12841 tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga
    12901 ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca
    12961 tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta
    13021 tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc
    13081 acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca
    13141 gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac
    13201 gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta
    13261 catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga
    13321 tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta
    13381 tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa
    13441 tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa
    13501 acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct
    13561 aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt
    13621 aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc
    13681 caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct
    13741 acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag
    13801 catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc
    13861 gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa
    13921 gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc
    13981 aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg
    14041 gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa
    14101 gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt
    14161 cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac
    14221 attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag
    14281 gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat
    14341 ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc
    14401 tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc
    14461 gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca
    14521 agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg
    14581 atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga
    14641 tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct
    14701 cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact
    14761 agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga
    14821 ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc
    14881 tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat
    14941 tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac atactctttt
    15001 tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc
    15061 agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa
    15121 tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat
    15181 caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc
    15241 gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc
    15301 tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa
    15361 ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat
    15421 gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg
    15481 actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat
    15541 cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac
    15601 attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc
    15661 tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac
    15721 tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt
    15781 atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc
    15841 aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga
    15901 taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa
    15961 gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga
    16021 agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg
    16081 tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct
    16141 accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg
    16201 gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt
    16261 tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat
    16321 cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta
    16381 tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc
    16441 ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat
    16501 cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac
    16561 gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt
    16621 atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga
    16681 ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa
    16741 tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga
    16801 ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata
    16861 ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg
    16921 acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt
    16981 ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt
    17041 aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg
    17101 agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac
    17161 gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct
    17221 ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc
    17281 tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac
    17341 tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt
    17401 ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg
    17461 ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca
    17521 ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta
    17581 ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg
    17641 aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct
    17701 tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca
    17761 ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc
    17821 ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa
    17881 attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct
    17941 tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta
    18001 caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt
    18061 gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt
    18121 gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg
    18181 ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca
    18241 tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt
    18301 tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt
    18361 ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat
    18421 tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga
    18481 tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc
    18541 gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa
    18601 taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt
    18661 cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc
    18721 ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg
    18781 tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt
    18841 atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa
    18901 gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc atatcctgct
    18961 gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg
    19021 tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga
    19081 acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt
    19141 gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc
    19201 ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg
    19261 acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact
    19321 tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc
    19381 acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg
    19441 gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg
    19501 gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac
    19561 atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga
    19621 ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga
    19681 atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa
    19741 aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt
    19801 ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat
    19861 ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac
    19921 gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag
    19981 ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg
    20041 ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg
    20101 caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc
    20161 caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg
    20221 cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca
    20281 cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt
    20341 cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca
    20401 gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt
    20461 cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta
    20521 catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa
    20581 tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg
    20641 tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc
    20701 cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca
    20761 taattactat cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt
    20821 ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc
    20881 tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct
    20941 gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata
    21001 taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt
    21061 agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat
    21121 ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat
    21181 cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc
    21241 atttttatga aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca
    21301 attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct
    21361 tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa
    21421 tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg
    21481 taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca
    21541 aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc
    21601 agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat
    21661 cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt
    21721 tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg
    21781 gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt
    21841 tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa
    21901 cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt
    21961 ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt
    22021 tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt
    22081 actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact
    22141 tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg
    22201 tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt
    22261 ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc
    22321 cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata
    22381 tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc
    22441 ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc
    22501 aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc
    22561 ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt
    22621 catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat
    22681 gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat
    22741 gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata
    22801 atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc
    22861 ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt
    22921 ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg
    22981 tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt
    23041 cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt
    23101 ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt
    23161 ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac
    23221 cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt
    23281 acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc
    23341 caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc
    23401 atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc
    23461 atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa
    23521 atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc
    23581 ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg
    23641 gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag
    23701 aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc
    23761 ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa
    23821 atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga
    23881 accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt
    23941 cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg
    24001 gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca
    24061 ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca
    24121 aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct
    24181 tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg
    24241 atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca taactttttg
    24301 aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc
    24361 atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca
    24421 tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct
    24481 aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct
    24541 tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga
    24601 taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc
    24661 agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat
    24721 ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa
    24781 gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc
    24841 catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag
    24901 ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg
    24961 agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca
    25021 tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc
    25081 tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt
    25141 tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt
    25201 cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat
    25261 cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac
    25321 tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct
    25381 aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca
    25441 ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat
    25501 tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg
    25561 ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca
    25621 ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc
    25681 tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct
    25741 tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga
    25801 ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa
    25861 atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta
    25921 tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca
    25981 tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa
    26041 tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac
    26101 ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa
    26161 tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg
    26221 ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac
    26281 agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa
    26341 tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg
    26401 ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc
    26461 ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca
    26521 ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa
    26581 caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta
    26641 gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg
    26701 atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt
    26761 tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga
    26821 tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag
    26881 ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg
    26941 ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg
    27001 ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata
    27061 accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa
    27121 ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata
    27181 aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg
    27241 cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg
    27301 gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc
    27361 ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga
    27421 aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga
    27481 caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc
    27541 aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt
    27601 tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt
    27661 gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc
    27721 acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat
    27781 tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa
    27841 aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc
    27901 tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca
    27961 tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc
    28021 aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt
    28081 gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt
    28141 ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca
    28201 tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct
    28261 agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga
    28321 acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg
    28381 ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg
    28441 aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag
    28501 tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc
    28561 acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac
    28621 atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca
    28681 aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc
    28741 ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta
    28801 tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca
    28861 aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc
    28921 cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc
    28981 ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag
    29041 acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta
    29101 tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc
    29161 gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt
    29221 agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa
    29281 ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc
    29341 acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta
    29401 agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag
    29461 attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat
    29521 cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt
    29581 attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta
    29641 caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa
    29701 aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt
    29761 caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga
    29821 tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat
    29881 tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt
    29941 ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg
    30001 aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt
    30061 acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg
    30121 tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc
    30181 ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca
    30241 attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa
    30301 ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct
    30361 gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag atatctctga
    30421 ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta
    30481 gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag
    30541 ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag
    30601 gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga
    30661 caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat
    30721 tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc
    30781 caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt
    30841 cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga
    30901 cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct
    30961 cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact
    31021 aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg
    31081 tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc
    31141 ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc
    31201 atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg
    31261 catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc
    31321 atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac
    31381 atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt
    31441 cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc
    31501 gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc
    31561 cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc
    31621 tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt
    31681 caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc
    31741 gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc
    31801 tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat
    31861 tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc
    31921 accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc
    31981 tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc
    32041 ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat
    32101 tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt
    32161 attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa
    32221 gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa
    32281 aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt
    32341 ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc
    32401 gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg
    32461 tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc
    32521 catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa
    32581 atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc
    32641 acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg
    32701 aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc
    32761 actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg
    32821 tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa
    32881 agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc
    32941 gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc
    33001 ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata
    33061 agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt
    33121 gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc
    33181 atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat
    33241 ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg
    33301 attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt
    33361 taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac
    33421 gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc
    33481 tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat
    33541 gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat
    33601 tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta
    33661 ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct
    33721 ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga
    33781 tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag
    33841 tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct
    33901 tttttgaagt aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg
    33961 cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat
    34021 ttcacatttt cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt
    34081 gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagctg
    34141 acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct
    34201 tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct
    34261 ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg
    34321 tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg
    34381 gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca
    34441 cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt
    34501 cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa
    34561 ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa
    34621 taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg
    34681 atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg
    34741 taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata
    34801 ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt
    34861 gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt
    34921 tctttgattc gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca
    34981 agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct
    35041 ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt
    35101 tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg
    35161 tttcaaccac attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa
    35221 ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg
    35281 caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc
    35341 ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga
    35401 aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt
    35461 tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt
    35521 tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc
    35581 taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc
    35641 gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca
    35701 attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca
    35761 tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac
    35821 cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac
    35881 cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga
    35941 aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg
    36001 ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat
    36061 cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc
    36121 catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag
    36181 ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc
    36241 cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta
    36301 acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt
    36361 tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca cggcttactg
    36421 tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac
    36481 cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag
    36541 ctgctgccca tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat
    36601 tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt
    36661 cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt
    36721 ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac
    36781 cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat
    36841 ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct
    36901 ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc
    36961 ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt
    37021 tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc
    37081 catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa
    37141 ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga
    37201 aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata
    37261 cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct
    37321 cgattgctac taattctgcg tattctttat cataaacata gtcttccact gttggtttat
    37381 cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt
    37441 tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt
    37501 tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct
    37561 agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg
    37621 tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag
    37681 ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc
    37741 gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct
    37801 acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt
    37861 gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc
    37921 tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca
    37981 atcaggaata caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact
    38041 tgggaagcat cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag
    38101 accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg
    38161 tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct
    38221 aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc
    38281 aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat
    38341 gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccaca
//
LOCUS       DAFBYI010000012        38110 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFBYI010000012 DAFBYI010000000
VERSION     DAFBYI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.012"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(451..1761)
                     /locus_tag="LLD04_000544"
     CDS             complement(451..1761)
                     /locus_tag="LLD04_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5647498.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LLD04_000545"
     CDS             complement(1773..3191)
                     /locus_tag="LLD04_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5647499.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="LLD04_000546"
     CDS             complement(3431..4999)
                     /locus_tag="LLD04_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5647500.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="LLD04_000547"
     CDS             complement(5003..5413)
                     /locus_tag="LLD04_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK5647501.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="LLD04_000548"
     CDS             complement(5410..6333)
                     /locus_tag="LLD04_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK5647502.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="LLD04_000549"
     CDS             complement(6330..7208)
                     /locus_tag="LLD04_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK5647503.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="LLD04_000550"
     CDS             complement(7195..8532)
                     /locus_tag="LLD04_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5647504.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="LLD04_000551"
     CDS             complement(8535..9638)
                     /locus_tag="LLD04_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5647505.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="LLD04_000552"
     CDS             complement(9712..11220)
                     /locus_tag="LLD04_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5647506.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNADGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="LLD04_000553"
     CDS             complement(11186..11878)
                     /locus_tag="LLD04_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5647507.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="LLD04_000554"
     CDS             complement(11900..12874)
                     /locus_tag="LLD04_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647508.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="LLD04_000555"
     CDS             13174..14046
                     /locus_tag="LLD04_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5647509.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="LLD04_000556"
     CDS             complement(14504..16702)
                     /locus_tag="LLD04_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647510.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="LLD04_000557"
     CDS             16934..17926
                     /locus_tag="LLD04_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647511.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="LLD04_000558"
     CDS             17986..19407
                     /locus_tag="LLD04_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK5647512.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="LLD04_000559"
     CDS             19400..20899
                     /locus_tag="LLD04_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK5647513.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="LLD04_000560"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="LLD04_000560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK5647514.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="LLD04_000561"
     CDS             22491..23174
                     /locus_tag="LLD04_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK5647515.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="LLD04_000562"
     CDS             23176..24012
                     /locus_tag="LLD04_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5647516.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="LLD04_000563"
     CDS             24023..25777
                     /locus_tag="LLD04_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647517.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="LLD04_000564"
     CDS             complement(26053..26394)
                     /locus_tag="LLD04_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5647518.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="LLD04_000565"
     CDS             complement(26462..28633)
                     /locus_tag="LLD04_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5647519.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="LLD04_000566"
     CDS             28802..29701
                     /locus_tag="LLD04_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5647520.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="LLD04_000567"
     CDS             complement(29772..30629)
                     /locus_tag="LLD04_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5647521.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="LLD04_000568"
     CDS             complement(30614..33316)
                     /locus_tag="LLD04_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK5647522.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="LLD04_000569"
     CDS             complement(33329..34618)
                     /locus_tag="LLD04_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK5647523.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="LLD04_000570"
     CDS             34768..35814
                     /locus_tag="LLD04_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647524.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="LLD04_000571"
     CDS             35816..37969
                     /locus_tag="LLD04_000571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK5647525.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11245 a         7841 c         6771 g        12253 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcagc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggc tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca
//
LOCUS       DAFBYI010000013        37288 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFBYI010000013 DAFBYI010000000
VERSION     DAFBYI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.013"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            107..970
                     /locus_tag="LLD04_000572"
     CDS             107..970
                     /locus_tag="LLD04_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5647526.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="LLD04_000573"
     CDS             complement(1099..2721)
                     /locus_tag="LLD04_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647527.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="LLD04_000574"
     CDS             2970..3491
                     /locus_tag="LLD04_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5647528.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="LLD04_000575"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="LLD04_000575"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK5647529.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="LLD04_000576"
     CDS             5572..6390
                     /locus_tag="LLD04_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK5647530.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="LLD04_000577"
     CDS             complement(6493..6690)
                     /locus_tag="LLD04_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647531.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="LLD04_000578"
     CDS             6859..7437
                     /locus_tag="LLD04_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5647532.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7532..7669
                     /locus_tag="LLD04_000579"
     CDS             7532..7669
                     /locus_tag="LLD04_000579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647533.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8482..8985)
                     /locus_tag="LLD04_000580"
     CDS             complement(8482..8985)
                     /locus_tag="LLD04_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5647534.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9343..10368
                     /locus_tag="LLD04_000581"
     CDS             9343..10368
                     /locus_tag="LLD04_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5647535.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10691..11569
                     /locus_tag="LLD04_000582"
     CDS             10691..11569
                     /locus_tag="LLD04_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK5647536.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12045..12161
                     /locus_tag="LLD04_000583"
     CDS             12045..12161
                     /locus_tag="LLD04_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5647537.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13089..13427
                     /locus_tag="LLD04_000584"
     CDS             13089..13427
                     /locus_tag="LLD04_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647538.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13521..13751
                     /locus_tag="LLD04_000585"
     CDS             13521..13751
                     /locus_tag="LLD04_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647539.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14151..14522
                     /locus_tag="LLD04_000586"
     CDS             14151..14522
                     /locus_tag="LLD04_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK5647540.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14796..15488)
                     /locus_tag="LLD04_000587"
     CDS             complement(14796..15488)
                     /locus_tag="LLD04_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5647541.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15491..16276)
                     /locus_tag="LLD04_000588"
     CDS             complement(15491..16276)
                     /locus_tag="LLD04_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5647542.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQDHYAQYVDLQIADSTENTTIGA"
     gene            16513..17913
                     /gene="lacG"
                     /locus_tag="LLD04_000589"
     CDS             16513..17913
                     /gene="lacG"
                     /locus_tag="LLD04_000589"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK5647543.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17938..18255
                     /locus_tag="LLD04_000590"
     CDS             17938..18255
                     /locus_tag="LLD04_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5647544.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18268..19941
                     /locus_tag="LLD04_000591"
     CDS             18268..19941
                     /locus_tag="LLD04_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK5647545.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19955..21586
                     /locus_tag="LLD04_000592"
     CDS             19955..21586
                     /locus_tag="LLD04_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK5647546.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="LLD04_000593"
     CDS             complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="LLD04_000593"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK5647547.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23701..24624)
                     /locus_tag="LLD04_000594"
     CDS             complement(23701..24624)
                     /locus_tag="LLD04_000594"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK5647548.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24820..25425
                     /locus_tag="LLD04_000595"
     CDS             24820..25425
                     /locus_tag="LLD04_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5647549.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25733..26749
                     /locus_tag="LLD04_000596"
     CDS             25733..26749
                     /locus_tag="LLD04_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5647550.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26890..28191
                     /locus_tag="LLD04_000597"
     CDS             26890..28191
                     /locus_tag="LLD04_000597"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK5647551.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28237..28899
                     /gene="deoC"
                     /locus_tag="LLD04_000598"
     CDS             28237..28899
                     /gene="deoC"
                     /locus_tag="LLD04_000598"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK5647552.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28920..29318
                     /locus_tag="LLD04_000599"
     CDS             28920..29318
                     /locus_tag="LLD04_000599"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK5647553.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29384..30466
                     /locus_tag="LLD04_000600"
     CDS             29384..30466
                     /locus_tag="LLD04_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5647554.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30678..32240
                     /locus_tag="LLD04_000601"
     CDS             30678..32240
                     /locus_tag="LLD04_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647555.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32233..33366
                     /locus_tag="LLD04_000602"
     CDS             32233..33366
                     /locus_tag="LLD04_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647556.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33366..34322
                     /locus_tag="LLD04_000603"
     CDS             33366..34322
                     /locus_tag="LLD04_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647557.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34421..35587
                     /gene="deoB"
                     /locus_tag="LLD04_000604"
     CDS             34421..35587
                     /gene="deoB"
                     /locus_tag="LLD04_000604"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK5647558.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35611..36429
                     /locus_tag="LLD04_000605"
     CDS             35611..36429
                     /locus_tag="LLD04_000605"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5647559.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36446..37153
                     /gene="deoD"
                     /locus_tag="LLD04_000606"
     CDS             36446..37153
                     /gene="deoD"
                     /locus_tag="LLD04_000606"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5647560.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11628 a         6508 c         7893 g        11259 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc tttttttgtt gggaatccgg tttctttaac
     9121 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     9181 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     9241 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     9301 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     9361 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     9421 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     9481 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     9541 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     9601 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     9661 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     9721 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     9781 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     9841 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     9901 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     9961 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
    10021 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
    10081 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
    10141 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
    10201 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
    10261 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
    10321 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
    10381 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
    10441 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
    10501 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
    10561 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa
    10621 gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt
    10681 catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa
    10741 tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat
    10801 tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga
    10861 tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt
    10921 tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc
    10981 attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca
    11041 tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta
    11101 cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa
    11161 agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga
    11221 tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga
    11281 actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc
    11341 caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg
    11401 attattatca gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga
    11461 tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt
    11521 gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag tttagtactt
    11581 cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
    11641 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    11701 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    11761 gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac
    11821 aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg
    11881 aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt
    11941 gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg
    12001 ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata
    12061 tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga
    12121 agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta
    12181 aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt
    12241 ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta gaatctttct
    12301 tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca
    12361 cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt
    12421 tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt
    12481 agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat
    12541 ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg
    12601 aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt
    12661 gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat
    12721 atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt
    12781 tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta
    12841 tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta
    12901 aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga
    12961 ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa
    13021 gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa
    13081 cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag
    13141 aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa
    13201 ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa
    13261 ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga
    13321 gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg
    13381 attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat
    13441 actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca
    13501 aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga
    13561 atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa
    13621 ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt
    13681 ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta
    13741 tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt tattgttttt
    13801 tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat
    13861 tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt
    13921 tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata atatttttga
    13981 tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa
    14041 tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc
    14101 ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata atgagagatt
    14161 acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg
    14221 ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata
    14281 tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta
    14341 tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat
    14401 taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt
    14461 tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt
    14521 aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag
    14581 attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa
    14641 cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga
    14701 tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat
    14761 ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac
    14821 aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc
    14881 tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc
    14941 gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga
    15001 gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa
    15061 gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca
    15121 aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg
    15181 tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt
    15241 tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag
    15301 gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata
    15361 ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc
    15421 caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc
    15481 cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga
    15541 catattgtgc atagtggtct tgaacctccc gcgagatctg gcgatccgta atgagcagat
    15601 cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa
    15661 cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg
    15721 tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa
    15781 actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc
    15841 ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa
    15901 ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt
    15961 cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg
    16021 tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt
    16081 cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg
    16141 atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga
    16201 tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc
    16261 tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac
    16321 aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa
    16381 tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat
    16441 gtttcggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga
    16501 ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc
    16561 tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt
    16621 ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca
    16681 aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg
    16741 agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat
    16801 cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt
    16861 gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca
    16921 tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc
    16981 tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga
    17041 atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc
    17101 cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca
    17161 ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac
    17221 gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa
    17281 accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa
    17341 gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac
    17401 taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg
    17461 ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa
    17521 ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg
    17581 ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg
    17641 gggtataaag atcagtttga agatggcatt attatggatc agccgagaat cgactatttg
    17701 agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat
    17761 tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc
    17821 ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac
    17881 aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg
    17941 aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc
    18001 agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg
    18061 gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta
    18121 gcggaagaag catctgggaa agagatggag atgggattca tttttatcca tgggcaggat
    18181 catttaatga ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga
    18241 cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg
    18301 aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt
    18361 ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct
    18421 gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat
    18481 tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat
    18541 tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc
    18601 tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa
    18661 tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac agttaattta
    18721 tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg
    18781 atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta
    18841 attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg
    18901 ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg
    18961 atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg
    19021 gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct
    19081 tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca
    19141 acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt
    19201 gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca
    19261 cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt
    19321 tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct
    19381 tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt
    19441 gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca
    19501 tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa
    19561 ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat
    19621 gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgcgatgtta
    19681 gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt
    19741 gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa
    19801 gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt
    19861 gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt
    19921 attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa
    19981 agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc
    20041 atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc
    20101 tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa
    20161 tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag
    20221 ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa
    20281 tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa
    20341 acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta
    20401 cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt
    20461 tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga
    20521 aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg
    20581 agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg
    20641 agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg
    20701 agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc
    20761 gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac
    20821 agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata
    20881 tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc
    20941 agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa
    21001 tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa
    21061 cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc
    21121 gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg
    21181 accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac
    21241 gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca
    21301 agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt
    21361 gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta
    21421 tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat
    21481 caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg
    21541 aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt
    21601 cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac
    21661 ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg
    21721 agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg
    21781 taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa
    21841 gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt
    21901 tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa
    21961 cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt
    22021 tagctattga agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat
    22081 tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg
    22141 gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca
    22201 aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa
    22261 gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc
    22321 cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg
    22381 cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga
    22441 ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca
    22501 cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct
    22561 ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg
    22621 ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt
    22681 tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt
    22741 tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt gactttgatg
    22801 atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt
    22861 agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga
    22921 accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca
    22981 acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct
    23041 tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga
    23101 cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca
    23161 atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag
    23221 tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga
    23281 tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa
    23341 ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct
    23401 aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg
    23461 ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg
    23521 ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac
    23581 tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc
    23641 aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg
    23701 ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat
    23761 atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt
    23821 ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata
    23881 ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca
    23941 tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac
    24001 gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc
    24061 aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac
    24121 aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc
    24181 catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg
    24241 atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg
    24301 agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc
    24361 gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa
    24421 gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat
    24481 atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc
    24541 ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg
    24601 gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt
    24661 ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa
    24721 tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga
    24781 tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct
    24841 gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc
    24901 tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt
    24961 gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg
    25021 ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa
    25081 atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa
    25141 atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat
    25201 gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg
    25261 acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa
    25321 caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc
    25381 acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag
    25441 tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt
    25501 gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag
    25561 agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag
    25621 tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc
    25681 ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct
    25741 gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt
    25801 gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa
    25861 tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac
    25921 catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt
    25981 agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca
    26041 agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct
    26101 ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat
    26161 gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa
    26221 ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat
    26281 cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg
    26341 tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt
    26401 gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc
    26461 tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg
    26521 caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata
    26581 catgattccg caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc
    26641 acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga
    26701 aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat
    26761 attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag
    26821 tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag
    26881 gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg
    26941 aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa
    27001 gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac
    27061 ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata
    27121 aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc
    27181 ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca
    27241 ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa
    27301 gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg
    27361 gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt
    27421 ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt
    27481 ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg
    27541 gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca
    27601 gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc
    27661 gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc
    27721 caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa
    27781 gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg
    27841 ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag
    27901 cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct
    27961 gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga
    28021 ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc
    28081 tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt
    28141 ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa
    28201 gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac
    28261 catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca
    28321 aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa
    28381 caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac
    28441 acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc
    28501 gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat
    28561 atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tgttgaaaca
    28621 tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct
    28681 gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt
    28741 ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac
    28801 gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga
    28861 gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca
    28921 tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg
    28981 ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc
    29041 aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt
    29101 ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca
    29161 cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc
    29221 ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg
    29281 cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg
    29341 actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt
    29401 atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa
    29461 cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat
    29521 cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga
    29581 aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc
    29641 ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac
    29701 gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac
    29761 aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc
    29821 aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga
    29881 aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc
    29941 tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt
    30001 gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat
    30061 gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt
    30121 ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga
    30181 ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt
    30241 gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga
    30301 cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct
    30361 aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat
    30421 catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag
    30481 ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta
    30541 tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc
    30601 aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt
    30661 taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca
    30721 aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa
    30781 ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg
    30841 gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa
    30901 gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg
    30961 atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc
    31021 ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag
    31081 tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt
    31141 taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga
    31201 cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt
    31261 cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag
    31321 ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat
    31381 tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc
    31441 ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag
    31501 ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg
    31561 atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg
    31621 gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac
    31681 atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat
    31741 tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc
    31801 tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa
    31861 caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta
    31921 tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac
    31981 tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca
    32041 aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg
    32101 ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg
    32161 aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg
    32221 gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt
    32281 tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc
    32341 atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa
    32401 acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca
    32461 gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc
    32521 tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc
    32581 ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt
    32641 ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca
    32701 tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg
    32761 atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc
    32821 gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag
    32881 aaaacgactt taggatttga aatccgttct gttggattga atccttttgc ttctgagtat
    32941 gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca
    33001 gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact
    33061 tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc
    33121 ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag
    33181 acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt
    33241 gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa
    33301 gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa
    33361 ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa
    33421 caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg
    33481 tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga
    33541 tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag
    33601 gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta
    33661 tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat
    33721 ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag
    33781 gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt
    33841 attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct
    33901 tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat
    33961 atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg
    34021 tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca
    34081 tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat
    34141 tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt
    34201 cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg
    34261 ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat
    34321 aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg
    34381 ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct
    34441 gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt
    34501 tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga
    34561 gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca
    34621 aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg
    34681 ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga
    34741 tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta
    34801 tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat
    34861 tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt
    34921 agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat
    34981 gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag
    35041 caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga
    35101 agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat
    35161 cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat
    35221 gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg
    35281 tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc
    35341 agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa
    35401 cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag
    35461 taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac
    35521 gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt
    35581 aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa
    35641 acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt
    35701 tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag
    35761 atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct
    35821 ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc
    35881 atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt
    35941 gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt
    36001 accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg
    36061 ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa
    36121 gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga
    36181 ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta
    36241 gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga
    36301 atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt
    36361 gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca
    36421 ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat
    36481 cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt
    36541 tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata
    36601 taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta
    36661 tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg
    36721 tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag
    36781 ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa
    36841 tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa
    36901 tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga
    36961 acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata
    37021 ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac
    37081 aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc
    37141 gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa
    37201 gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt
    37261 tgaacaccgt ttaccaagag gttgtgaa
//
LOCUS       DAFBYI010000014        35657 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFBYI010000014 DAFBYI010000000
VERSION     DAFBYI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.014"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..2198)
                     /locus_tag="LLD04_000607"
     CDS             complement(<1..2198)
                     /locus_tag="LLD04_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647561.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="LLD04_000608"
     CDS             complement(2185..2403)
                     /locus_tag="LLD04_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647562.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="LLD04_000609"
     CDS             complement(2405..3448)
                     /locus_tag="LLD04_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5647563.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="LLD04_000610"
     CDS             complement(3603..3929)
                     /locus_tag="LLD04_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647564.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="LLD04_000611"
     CDS             complement(3937..4140)
                     /locus_tag="LLD04_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647565.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="LLD04_000612"
     CDS             4215..4865
                     /locus_tag="LLD04_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5647566.1"
                     /translation="MSIKTLLNLWFLCYDSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="LLD04_000613"
     CDS             4920..6086
                     /locus_tag="LLD04_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5647567.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="LLD04_000614"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="LLD04_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5647568.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="LLD04_000615"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="LLD04_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK5647569.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="LLD04_000616"
     CDS             complement(7545..8012)
                     /locus_tag="LLD04_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK5647570.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="LLD04_000617"
     CDS             complement(8132..9670)
                     /locus_tag="LLD04_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK5647571.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="LLD04_000618"
     CDS             9750..10436
                     /locus_tag="LLD04_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK5647572.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="LLD04_000619"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="LLD04_000619"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5647573.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="LLD04_000620"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="LLD04_000620"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5647574.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="LLD04_000621"
     CDS             complement(11922..13052)
                     /locus_tag="LLD04_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5647575.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="LLD04_000622"
     CDS             complement(13052..15235)
                     /locus_tag="LLD04_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5647576.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="LLD04_000623"
     CDS             complement(15232..16257)
                     /locus_tag="LLD04_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5647577.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="LLD04_000624"
     CDS             complement(16302..17129)
                     /locus_tag="LLD04_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5647578.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="LLD04_000625"
     CDS             complement(17126..18193)
                     /locus_tag="LLD04_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK5647579.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="LLD04_000626"
     CDS             complement(18219..19055)
                     /locus_tag="LLD04_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5647580.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="LLD04_000627"
     CDS             complement(19052..19921)
                     /locus_tag="LLD04_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647581.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="LLD04_000628"
     CDS             complement(19991..21274)
                     /locus_tag="LLD04_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK5647582.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="LLD04_000629"
     CDS             complement(21288..22973)
                     /locus_tag="LLD04_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK5647583.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="LLD04_000630"
     CDS             23248..24273
                     /locus_tag="LLD04_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647584.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28542)
                     /gene="rpoC"
                     /locus_tag="LLD04_000631"
     CDS             complement(24868..28542)
                     /gene="rpoC"
                     /locus_tag="LLD04_000631"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK5647585.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="LLD04_000632"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="LLD04_000632"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5647586.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="LLD04_000633"
     CDS             32945..33913
                     /locus_tag="LLD04_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5647587.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="LLD04_000634"
     CDS             33931..34836
                     /locus_tag="LLD04_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647588.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="LLD04_000635"
     CDS             34833..35639
                     /locus_tag="LLD04_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647589.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10106 a         7530 c         6436 g        11585 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctatgata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctccctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaaaga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tgatttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggac
//
LOCUS       DAFBYI010000015        34731 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFBYI010000015 DAFBYI010000000
VERSION     DAFBYI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.015"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            232..1500
                     /locus_tag="LLD04_000636"
     CDS             232..1500
                     /locus_tag="LLD04_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBK5647590.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="LLD04_000637"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="LLD04_000637"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK5647591.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="LLD04_000638"
     CDS             3076..3351
                     /locus_tag="LLD04_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK5647592.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="LLD04_000639"
     CDS             3357..4124
                     /locus_tag="LLD04_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK5647593.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="LLD04_000640"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="LLD04_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK5647594.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="LLD04_000641"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="LLD04_000641"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK5647595.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="LLD04_000642"
     CDS             8268..8570
                     /locus_tag="LLD04_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK5647596.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="LLD04_000643"
     CDS             8573..9736
                     /locus_tag="LLD04_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5647597.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="LLD04_000644"
     CDS             9762..13190
                     /locus_tag="LLD04_000644"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK5647598.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="LLD04_000645"
     CDS             13211..14335
                     /locus_tag="LLD04_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647599.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="LLD04_000646"
     CDS             14325..14762
                     /locus_tag="LLD04_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5647600.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="LLD04_000647"
     CDS             14746..15084
                     /locus_tag="LLD04_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK5647601.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="LLD04_000648"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="LLD04_000648"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK5647602.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="LLD04_000649"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="LLD04_000649"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK5647603.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="LLD04_000650"
     CDS             16241..17284
                     /locus_tag="LLD04_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK5647604.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="LLD04_000651"
     CDS             17443..18171
                     /locus_tag="LLD04_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK5647605.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="LLD04_000652"
     CDS             18287..18532
                     /locus_tag="LLD04_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5647606.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="LLD04_000653"
     CDS             18529..18747
                     /locus_tag="LLD04_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK5647607.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="LLD04_000654"
     CDS             18883..19422
                     /locus_tag="LLD04_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK5647608.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="LLD04_000655"
     CDS             19415..21688
                     /locus_tag="LLD04_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK5647609.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="LLD04_000656"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="LLD04_000656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK5647610.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="LLD04_000657"
     CDS             complement(22861..23115)
                     /locus_tag="LLD04_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK5647611.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="LLD04_000658"
     CDS             complement(23515..23784)
                     /locus_tag="LLD04_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647612.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="LLD04_000659"
     CDS             complement(24254..24709)
                     /locus_tag="LLD04_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK5647613.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="LLD04_000660"
     CDS             complement(24847..26775)
                     /locus_tag="LLD04_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK5647614.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="LLD04_000661"
     CDS             complement(26972..27805)
                     /locus_tag="LLD04_000661"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK5647615.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="LLD04_000662"
     CDS             complement(27997..28701)
                     /locus_tag="LLD04_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5647616.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="LLD04_000663"
     CDS             complement(28724..29350)
                     /locus_tag="LLD04_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5647617.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="LLD04_000664"
     CDS             29676..32051
                     /locus_tag="LLD04_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK5647618.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="LLD04_000665"
     CDS             complement(32075..32566)
                     /locus_tag="LLD04_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK5647619.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="LLD04_000666"
     CDS             32753..33673
                     /locus_tag="LLD04_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5647620.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="LLD04_000667"
     CDS             33827..34171
                     /locus_tag="LLD04_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK5647621.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..>34731)
                     /locus_tag="LLD04_000668"
     CDS             complement(34361..>34731)
                     /locus_tag="LLD04_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647622.1"
                     /translation="KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNET
                     LRNGAGHIGYGISPNYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQK
                     NGAAIYREDEKEYYTKIKLM"
BASE COUNT        11488 a         6081 c         7197 g         9965 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaaggc gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgttgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc ggttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt a
//
LOCUS       DAFBYI010000016        33947 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFBYI010000016 DAFBYI010000000
VERSION     DAFBYI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.016"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(35..2200)
                     /locus_tag="LLD04_000669"
     CDS             complement(35..2200)
                     /locus_tag="LLD04_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5647623.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2460..4754
                     /locus_tag="LLD04_000670"
     CDS             2460..4754
                     /locus_tag="LLD04_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5647624.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4747..5412
                     /gene="pgmB"
                     /locus_tag="LLD04_000671"
     CDS             4747..5412
                     /gene="pgmB"
                     /locus_tag="LLD04_000671"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5647625.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5405..6412
                     /locus_tag="LLD04_000672"
     CDS             5405..6412
                     /locus_tag="LLD04_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5647626.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6434..7453
                     /locus_tag="LLD04_000673"
     CDS             6434..7453
                     /locus_tag="LLD04_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647627.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(7734..9101)
                     /gene="nhaC"
                     /locus_tag="LLD04_000674"
     CDS             complement(7734..9101)
                     /gene="nhaC"
                     /locus_tag="LLD04_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK5647628.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(9210..10631)
                     /locus_tag="LLD04_000675"
     CDS             complement(9210..10631)
                     /locus_tag="LLD04_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647629.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(10874..12751)
                     /gene="tdc"
                     /locus_tag="LLD04_000676"
     CDS             complement(10874..12751)
                     /gene="tdc"
                     /locus_tag="LLD04_000676"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5647630.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(13042..14298)
                     /locus_tag="LLD04_000677"
     CDS             complement(13042..14298)
                     /locus_tag="LLD04_000677"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK5647631.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(14410..14615)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14878..16179
                     /locus_tag="LLD04_000678"
     CDS             14878..16179
                     /locus_tag="LLD04_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5647632.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(16272..17660)
                     /gene="ltrA"
                     /locus_tag="LLD04_000679"
     CDS             complement(16272..17660)
                     /gene="ltrA"
                     /locus_tag="LLD04_000679"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5647633.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(18147..18746)
                     /locus_tag="LLD04_000680"
     CDS             complement(18147..18746)
                     /locus_tag="LLD04_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5647634.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            19000..19881
                     /locus_tag="LLD04_000681"
     CDS             19000..19881
                     /locus_tag="LLD04_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647635.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            19894..21504
                     /locus_tag="LLD04_000682"
                     /pseudo
     CDS             19894..21504
                     /locus_tag="LLD04_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(21581..22240)
                     /locus_tag="LLD04_000683"
     CDS             complement(21581..22240)
                     /locus_tag="LLD04_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5647636.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(22523..23188)
                     /locus_tag="LLD04_000684"
     CDS             complement(22523..23188)
                     /locus_tag="LLD04_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK5647637.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(23335..23940)
                     /gene="dhaL"
                     /locus_tag="LLD04_000685"
     CDS             complement(23335..23940)
                     /gene="dhaL"
                     /locus_tag="LLD04_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK5647638.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(23968..24978)
                     /gene="dhaK"
                     /locus_tag="LLD04_000686"
     CDS             complement(23968..24978)
                     /gene="dhaK"
                     /locus_tag="LLD04_000686"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK5647639.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(24953..25399)
                     /gene="dhaM"
                     /locus_tag="LLD04_000687"
     CDS             complement(24953..25399)
                     /gene="dhaM"
                     /locus_tag="LLD04_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK5647640.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            25502..25821
                     /locus_tag="LLD04_000688"
                     /pseudo
     CDS             25502..25821
                     /locus_tag="LLD04_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
     gene            complement(25933..26802)
                     /locus_tag="LLD04_000689"
     CDS             complement(25933..26802)
                     /locus_tag="LLD04_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5647641.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(26898..28202)
                     /locus_tag="LLD04_000690"
     CDS             complement(26898..28202)
                     /locus_tag="LLD04_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5647642.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(28199..28708)
                     /locus_tag="LLD04_000691"
     CDS             complement(28199..28708)
                     /locus_tag="LLD04_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647643.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(28883..31018)
                     /locus_tag="LLD04_000692"
     CDS             complement(28883..31018)
                     /locus_tag="LLD04_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5647644.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(31101..32756)
                     /locus_tag="LLD04_000693"
     CDS             complement(31101..32756)
                     /locus_tag="LLD04_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5647645.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(32758..33072)
                     /locus_tag="LLD04_000694"
     CDS             complement(32758..33072)
                     /locus_tag="LLD04_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5647646.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            33242..33502
                     /locus_tag="LLD04_000695"
     CDS             33242..33502
                     /locus_tag="LLD04_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK5647647.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            33516..33830
                     /locus_tag="LLD04_000696"
     CDS             33516..33830
                     /locus_tag="LLD04_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK5647648.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         9773 a         7168 c         6193 g        10813 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt
       61 taccagctgt cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa
      121 atacgatcat gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag
      181 ctaatttcgt tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct
      241 gattcatttg gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt
      301 ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa
      361 caacttctct tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata
      421 ccggatcttt tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt
      481 gttctatacc taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg
      541 gctctggaat ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg
      601 cttttggtcc ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg
      661 ctcgtttcca ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc
      721 gagtcataca agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct
      781 ggaccacttg ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt
      841 cattgtcgta atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat
      901 ttgctaagaa atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat
      961 ctccacctag acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa
     1021 atgaatggac ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg
     1081 aataaatgac atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta
     1141 cacctgttag aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct
     1201 tatcaggatc gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac
     1261 caatcatttg tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt
     1321 gcgacatatc ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg
     1381 gatcttgccc aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc
     1441 caagctgtgt atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta
     1501 aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg
     1561 caatccataa accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa
     1621 tcaatgccac gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga
     1681 aaaatgacaa agcatccggc aattttctat aattatagta cttattataa gccattgccc
     1741 cgacaaatcc agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata
     1801 cacttgtaaa aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat
     1861 cttcattcgc aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta
     1921 aaataaatga gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc
     1981 ctattgctaa agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa
     2041 ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc
     2101 ctatactgat catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc
     2161 caaatttttg ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc
     2221 aatcggtttc gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt
     2281 tatttttctg tctataccat tctataaaac ctataatacg tgtgttccct tctataattt
     2341 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
     2401 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
     2461 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
     2521 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
     2581 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
     2641 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
     2701 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
     2761 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
     2821 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
     2881 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
     2941 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
     3001 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
     3061 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
     3121 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
     3181 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
     3241 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
     3301 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
     3361 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
     3421 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
     3481 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
     3541 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
     3601 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
     3661 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
     3721 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
     3781 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
     3841 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
     3901 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
     3961 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
     4021 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
     4081 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
     4141 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
     4201 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
     4261 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
     4321 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
     4381 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
     4441 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
     4501 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
     4561 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
     4621 tctcagtcac agatgaacaa gttactttcg agttattaga aggttctcct ctagaaattt
     4681 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
     4741 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
     4801 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
     4861 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
     4921 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
     4981 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
     5041 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
     5101 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
     5161 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
     5221 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
     5281 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
     5341 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat
     5401 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
     5461 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
     5521 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
     5581 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
     5641 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
     5701 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
     5761 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
     5821 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
     5881 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
     5941 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
     6001 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
     6061 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
     6121 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
     6181 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
     6241 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
     6301 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
     6361 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
     6421 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
     6481 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
     6541 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
     6601 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
     6661 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
     6721 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
     6781 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac
     6841 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
     6901 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
     6961 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
     7021 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
     7081 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
     7141 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
     7201 gcagttggcg caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
     7261 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
     7321 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
     7381 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
     7441 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
     7501 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
     7561 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     7621 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
     7681 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
     7741 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
     7801 gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
     7861 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
     7921 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
     7981 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
     8041 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
     8101 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
     8161 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
     8221 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
     8281 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
     8341 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
     8401 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
     8461 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
     8521 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
     8581 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
     8641 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
     8701 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
     8761 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
     8821 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
     8881 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
     8941 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
     9001 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
     9061 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
     9121 atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg
     9181 acaaaacgtc ggctgaaaga cttatttttt tatttttctt ctaaactttt ttctacctca
     9241 atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact
     9301 gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg
     9361 acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt
     9421 ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa
     9481 acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag
     9541 aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt
     9601 gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat
     9661 tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc
     9721 caagctgata attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc
     9781 acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat
     9841 tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa
     9901 actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa
     9961 cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag
    10021 tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct
    10081 agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg
    10141 atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata
    10201 tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg
    10261 aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa
    10321 gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca
    10381 aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga
    10441 agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca
    10501 aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc
    10561 gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca
    10621 gatttactca ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag
    10681 tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt tgagtttatt
    10741 gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc
    10801 ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac
    10861 atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca
    10921 atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt
    10981 tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt
    11041 cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga
    11101 tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg
    11161 aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt
    11221 tttctttgaa tacatagtct accatattga aatctggata agttaatgga tgaacttcga
    11281 tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac
    11341 cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt
    11401 gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc
    11461 caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa
    11521 cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca
    11581 ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta
    11641 cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt
    11701 ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc
    11761 cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac
    11821 gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac
    11881 cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt
    11941 cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga
    12001 taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt
    12061 ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt
    12121 tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc
    12181 aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt
    12241 tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt
    12301 ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca
    12361 tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa
    12421 aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc
    12481 cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat
    12541 cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa
    12601 taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat
    12661 tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta
    12721 ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa
    12781 ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat
    12841 ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta
    12901 aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc
    12961 agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc
    13021 aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc
    13081 ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa
    13141 atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg
    13201 acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct
    13261 tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc
    13321 tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc
    13381 actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg
    13441 ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa
    13501 tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa
    13561 tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt
    13621 tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc
    13681 taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat
    13741 ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg
    13801 gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc
    13861 taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat
    13921 ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt
    13981 tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac
    14041 acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat
    14101 atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc
    14161 gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc
    14221 aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa
    14281 ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct
    14341 gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg
    14401 gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca
    14461 acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat
    14521 gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc
    14581 tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga
    14641 gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct
    14701 tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact
    14761 ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc
    14821 ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg
    14881 gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt
    14941 ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa
    15001 aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca
    15061 aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt
    15121 aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa
    15181 ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg
    15241 gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact
    15301 gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat
    15361 ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta
    15421 cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg
    15481 gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc
    15541 tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc
    15601 tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca
    15661 ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa
    15721 acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg
    15781 agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta
    15841 aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt
    15901 ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa
    15961 aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca
    16021 ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc
    16081 attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc
    16141 catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaatcg
    16201 agtagagggg aaagccaact gaccttccgc ccctctcaca ccaccgtacg tacggacccg
    16261 tatacggcgg ttcaataagt tgagtgtacg aactggtact tcaaggagat gtcctcatat
    16321 ccttggcggg ccagttcttt gtctgttaat gatcgatgaa gaatatgact tttggagact
    16381 gaccaaatac cttttcttgt atttgaccat tcatacgctt tcgctttatc gatccctaaa
    16441 cgaatcagat tttttctttt ggttcttgga ttcttccatt gtttccaaat atattgtctg
    16501 attcttcgct tcaaccaacc gtttaactcc ctcatgaatt ctttcatctc accaattcca
    16561 tagtagttga tccaaccagt cattagttgc ttgatttctt ttaaaataag ttggatactt
    16621 cttccacggt ttcttttagt gagttgtttg agcttgcttt tcacacgctg ttttgctttt
    16681 gcgtgagggc gaattttaat acctttcttt gttggcagaa tgcagaatcc aagaaacttt
    16741 cgtttcaatg gacttcctac cgcactcttt tctcgattta ccgtcaactt tagttgttct
    16801 tcgagaaatt gaatggattt cttcatcaca cgctgtcctg ctcgtttgct tctaacatag
    16861 atattgcagt catcggcgta tcgaacaaat ttatgtccta gattttctag cacttgatca
    16921 aattcgttca ggtagatgtt acttaacagg ggagagagat tgcctccttg gggcgttcct
    16981 tcttcagttg ttgtgatgag tccattttcc attacaccac tcattaagta ttgacggatc
    17041 agttttagga cttgtttgtc ctttactttc cgctcaatga aatacatcag cttatcgtga
    17101 tttacggtgt caaagtaggc tttcatatca atgtccacca catatcggta accttgttca
    17161 taataagatt ttgctttctt cactgcgtca tgtgcgctgc gatttgggcg aaacccataa
    17221 ctattatctg agaattctgg ttcaaatagc ggttgtaaca cttgattgat tgcctgttga
    17281 agcatacgat caataaccgt gggaattcct agttttctta caccgccgtt tggtttcggt
    17341 atttctactc gcaaaacagg ctgaggttta tagttccctt ggcgtaaatt ggatagtaat
    17401 tcctcgcggt gctctttcaa gtaaggaagc agttgatcga cggtcatccc atctgcacca
    17461 gccgcccctt tatttctgac tacttgaaga tacgcttgat tgagattatt ccgatctaga
    17521 attctcccaa acagattttc tacaccatct tgtttcctct gttcaccgca agaagtgctg
    17581 cgcacttcat catacccttc gtgttccaca ctagctcttt gatgatagcc aagttttctt
    17641 gttttctgcg gtcgtcgcat cttctcacac ctcctaaatt attcaagatt cttattgttc
    17701 agtccttcgt aacggtcagt gtcgttacta ctatgacctc ggctgacttc tcatgattca
    17761 ccatcatgtc gccatgatcg ttgtgctggt ttgttttcat tcaacgccca caacaccctc
    17821 atgagatctc ccccggtaag agtgaaaact ttcctcccat gtaactgcta gatttactgt
    17881 ataggtttcg ggcagtattg gactttgtct tgtgatgaag acttatccga cctaattcag
    17941 ccttgtatct agtttctgtt cgtcagttca ggagtttgcg tccgacttcc ttcagccaca
    18001 acctcacgat tgccgccttg tctttcgcta acagttccta ctgccaagcc tgtagtggac
    18061 tttcaccacc aagttttcac ccatgcaggg cgcacaccaa aaaagccgaa ctgacatttt
    18121 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
    18181 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
    18241 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
    18301 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
    18361 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
    18421 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
    18481 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
    18541 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
    18601 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
    18661 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
    18721 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
    18781 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
    18841 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
    18901 aaaataataa aaaaagtttt ataaaaaggt ataaaacctt tttattaaac tatttaatct
    18961 tgtttagtat atatttttag aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc
    19021 acacatttga aaaagagctt tggtcgtttc gaagctttaa aagatgtctc tttcaccgta
    19081 cattcaggtg aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc cacgacgatt
    19141 cggacacttt tagggattat aaaaaaagac ggcggaacgg caacgatttt tgggcaagat
    19201 gtatgggaag atagtttatc tatccatgaa cgtctttctt atgttccagg cgatatctct
    19261 ttgtggggaa atttgactgg aggagcgatc atcgatctct tcatgaagct tcatggaaaa
    19321 gggagtgctc aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc taagaaaaaa
    19381 gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct tgattgctgc tttggctgtt
    19441 gattcggatc tctatttatt tgatgagcct acttctggtt tagatccttt gatggaaacg
    19501 attttccaag aagaagttga aaaattgaaa gaacagggca aatccatcct actttcttct
    19561 catattctca gtgaagtcga gcgactagcg gataaagtcg tgatcatacg cgaaggaaca
    19621 ctcgatgaag aaggaacact cgatgaattg cgtcatttga ctcgttcgac catcactata
    19681 gaaacagaag cagagaatag tcacttagca caattgaacg gtgtccatga ctttgtgcaa
    19741 aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat tttagctgaa
    19801 gcgtccaaat taggtgtgaa aaaatttgaa tctattccac cgactcttga ggatttattc
    19861 atgagacatt atgaaggatg aggaggaagc gaaatgactg ataaattttc acgctggtcc
    19921 accctttttt ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg gctagtcggt
    19981 gtcactgctt tttctggtgg ttttgtccct gcgtttgaag aattgacttc cggacaagga
    20041 ttagctggat tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt cggaccaact
    20101 ccagcttcga atgctgctga gtatacttta ggagcgatgt atgcaaatga aatgttgctt
    20161 ttttgcagtt tgtttgctgc tgtgatctct gttttacacg tggtgagcca aacgagaaaa
    20221 aaagaagaac ttggtctgat tgaacttatt cgttcctttc gagtcggacg tcaagcaaac
    20281 tctttagctg tcatcgctga aagtattctg atcaatttgc ttttagcaat atcgatcagt
    20341 tgtacgatga tttttttcca agcggattcg atcactgcag aaggtgcttg gctttttggg
    20401 ctatctgtag gtagtatggg gattttgggg agtgccgttg cactgtttgc tgcacaactg
    20461 atgccaagtt cagccagttc gaatggtctg tctttagcgt ttttaggtgg actttacctt
    20521 ctacgagcaa taacagatgt agctgattcc tccctttcga tgtggaaacc tttaggctgg
    20581 tcttatttga cctatccatt tacaactaac aactgaattc ctttgcttta tttgtttttg
    20641 ttcagtctaa tcttgataat cagttcattt gttttagagg aacatcgaga tttgaacacc
    20701 ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac gttctctttt atccttgcgt
    20761 ggtttattat tctatctaaa taaagggatg acaatcagtt ggcttatcac ttttggtttg
    20821 atgggggcgg catacggctc tatctacggg aatatgcaac agtttttaga aagtaacgac
    20881 ttaatgaaac agatgtttat ccaatctggc gtctcaattg aaaaaaactt tacaagcacg
    20941 atcatggtcg tcttgatttg tctagctgct attttaccaa ttgctagcat caatcgttta
    21001 tttacagaag aaacccacgg acgtatgaat cagctgttcg caacaaaaat ttctaggaac
    21061 caactttatt ggaccaatat gttactagct attctatttg ctatattggc aatcctgatt
    21121 agttctggtg cactagggct ttctgctttg gcttcgatga aggatgcgaa agacatggat
    21181 ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt gtttatcgga
    21241 attagtggct ttctattcgg atggcttcct cgaatcggta aacttgctta tatctactta
    21301 gcttactcct ttgcactcaa ttactttggc ggaattttgg atttaccaga atggttttcc
    21361 aaaacagctg tccctagctg ggttccacgg atgccaaacg attcttttga tctttccgtg
    21421 ttccttacgt tatcatgtat cagccttgca ctgttcgtcc ttggttctat tggttatagg
    21481 cgaagagact tactagaagg ataataaaaa agcatgtaca atttcatgct agcctccaaa
    21541 tctgggtcaa aaatccaaca tttggaggtc atttttttat ttacaagtct tctaactgct
    21601 ctttatcaat gatccagact tctcgattct ttctttttac ataaccattc tcctctaata
    21661 atttaaattt tctagataaa gtttcggggg tcgtaccgat aaatgcagca agttctttca
    21721 ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt agctaagtca agcagataga
    21781 cagcgactcg ttcttctact ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg
    21841 ttaatttctc aacgttgatt gctaaaagtt tcagactaag ggcgggataa tgcagtaaga
    21901 gttccgagaa atctttttgt ctcaatatgc aaatttccgt tttttctagt gcttcgccaa
    21961 ataaagactc atttggaacg cctagcaact ggctctctcc ctcatatccg cccggctcta
    22021 ttactcgtaa aagctgttcc tttccgctag cagataattg atacactttc atactgccgg
    22081 aggccacgat cactaattga ggttctccat tcggcgcaag aatctgttct cctttttcca
    22141 catagtgatg tttgacaagt gcgtgtactt tttcttgatc ttgctgtact aagtgattga
    22201 acaaaggaac aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac
    22261 agctatttct aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca
    22321 tagcataaac gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt
    22381 gctttatttt ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt
    22441 tactttttga ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt
    22501 cgcattgctt atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg
    22561 gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat
    22621 agaagaatag tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt
    22681 gtttctttgt atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg
    22741 gttgcggcca tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg
    22801 caagcaactg gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt
    22861 acatcaaata gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt
    22921 cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg
    22981 tccagctttc gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct
    23041 ggagctgaat ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa
    23101 tcttatctgt tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca
    23161 actgataatc tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc
    23221 tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc
    23281 ataagaacag ccaaacaaaa aactgcgatg agcatcaccg cagttttatt gaacttaagg
    23341 ttgattgatc acatctaaaa gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc
    23401 gatatgtccg attgcacgct ctcctagata ggaagctcgt ccttttttag ctaccaagac
    23461 tttggtgtgt tcttttgcct ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc
    23521 ctctgtgact ggcagccaaa catctaacat cgttttatcg tcagcttctg cctttcctcg
    23581 gctttgaata cccgctgttc cctgctcgat cacttcgcct aatttttctt gagaatcaat
    23641 agaatcgacc tcttttgtcg ctttcatcat gttcatgaat gcagatccgt ataacggacc
    23701 agaagcccca ccgactttcg atatcatcgc catagagaat actttgaatg tatcgctgat
    23761 tgtttcagga tgatcggctt gaaaagcttc ttcataagct gaaacccctc ttcccatgtt
    23821 atttccatgg tctccatcgc caatcggggt atctagatca ctgagatatt gtttgttctg
    23881 attgattgcc tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat
    23941 attatcaccc ttttatcttg agatttttta ccatgaaatc gtctgtacat tgctttctaa
    24001 agaatctttc cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc
    24061 tagtgaggtc ataaaattac cgacttttcg gaaaactaca ttgacgcctc tttcttccaa
    24121 cagagctaaa acatcattca tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc
    24181 gttgactagt acacctgctt cttttggtaa attcgagtaa tcatctaaaa tcttagtaac
    24241 aagttccttt gccagtactt tagaaggctg catcttttct cgacgatagc ctggctcacc
    24301 gtggatacca acaccatact cgatctcatc ttctcctagt tcgaatcctg gtttccctac
    24361 ttctggtaca gttgctgctc gaagcgctag accgatcgtc ttagttgcct gtacgacctt
    24421 ttctcccagt tctttcaact cagctaggga agcaccgttt cttgctgcat cgcccacgat
    24481 tttgtgtaca aggacagtac ctgcaactcc gcgttttcca gcagtatacg tactattttc
    24541 aacggcgata tcatcatcta cgatcacact ttctacttca atatcgtcca tagcagctaa
    24601 ttcctttgcc atatcgaagt tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac
    24661 tccttttcca ctatcggctt ccttaatagc cgtctgaatc tgatctggag ttggagaagt
    24721 aaacacatca ccaagcaccg ctgcacttaa catcccttcc cctacaaacc cggcatggga
    24781 tggttcatgt ccgcttcctc caccagaaac caaaccaact tgttccgctt tctgattctt
    24841 ggctacaaca cgggaagatt ctacacgatg aacaagttct ggatagcttt ttactaatcc
    24901 ttccagcatt tcttcgacaa tattgcttgg atcattcatg atttttttca atttattccc
    24961 atcctttctt tccggcattt tgtgcttctg ttaagatttg ctgagtatca tctgttatac
    25021 cggcagtgat ggctgctgca aaagctcctt ccactaaagg cgcatccacc agatgataac
    25081 gttgctgctg ctcttcttcc aacatgtctt ttgccagttc cgagttcaat accgcgctac
    25141 cgatatccgc aaaaacaaaa taactagctg catcgcttga ttcattgact gcttctacga
    25201 ttttcatcgg gtctgatccg atctcgccat ttattccgcc gagagaaaaa atttgcacct
    25261 cttcggattt ttgcatctgc tcaatcattt ctttaatacc atctgttact ttttgactat
    25321 gagaaataag caaaatactt tttttcacaa gaatcttcct ttcttttaca tagttgctaa
    25381 cgattggttt cgctttcatt aatgaacaat tcaagtatat aataaatttc taattttggg
    25441 aaaatatgtg tagtgcttct tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg
    25501 aatgattaca acaacaacga acagtgtaga gggaaaagcc gtagtagctt ataaagggat
    25561 cgtttttggt gaggtcatta ctgggatcca tgcatttaaa gacttggagg cggggttgcg
    25621 aaatatcttt ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga
    25681 agcacttgcc gaaatggccg aacgtgcaaa gcattaggag ctgatgcgat cattggcgta
    25741 aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac atgtagtgga
    25801 acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag agagatggga
    25861 catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact ctaccttccc
    25921 atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc
    25981 cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg
    26041 acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt
    26101 cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa
    26161 gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg
    26221 gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt
    26281 ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag
    26341 atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc
    26401 acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc
    26461 ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc
    26521 caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc
    26581 tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc
    26641 cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg
    26701 ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg
    26761 ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact
    26821 aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct
    26881 aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca
    26941 taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcact
    27001 atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga
    27061 tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg
    27121 aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa
    27181 tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata
    27241 ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc
    27301 acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt
    27361 cgatatagca tcttcaacgt cacaggaact tccatgaaaa agataatctc aggagcaaaa
    27421 acagataacc agtcatttaa ctgatgatcc caagcaatac tgatttcttc tgtcgctcga
    27481 tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca
    27541 ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata
    27601 cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata
    27661 tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta
    27721 atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt
    27781 gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc
    27841 acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt
    27901 gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac
    27961 tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac
    28021 ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta tataaacaca
    28081 taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa
    28141 aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc
    28201 atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt
    28261 tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt
    28321 ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa
    28381 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
    28441 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
    28501 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
    28561 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
    28621 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
    28681 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
    28741 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
    28801 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
    28861 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
    28921 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
    28981 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
    29041 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
    29101 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
    29161 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
    29221 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
    29281 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
    29341 agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
    29401 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
    29461 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
    29521 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
    29581 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
    29641 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
    29701 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
    29761 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
    29821 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
    29881 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
    29941 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
    30001 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
    30061 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
    30121 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
    30181 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
    30241 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
    30301 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
    30361 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
    30421 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
    30481 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
    30541 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
    30601 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
    30661 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
    30721 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
    30781 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
    30841 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
    30901 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
    30961 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
    31021 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
    31081 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
    31141 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
    31201 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
    31261 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
    31321 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
    31381 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
    31441 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
    31501 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
    31561 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
    31621 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
    31681 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
    31741 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
    31801 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
    31861 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
    31921 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
    31981 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
    32041 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
    32101 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
    32161 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
    32221 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
    32281 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
    32341 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
    32401 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
    32461 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
    32521 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
    32581 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
    32641 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
    32701 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
    32761 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
    32821 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
    32881 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
    32941 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
    33001 tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
    33061 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
    33121 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
    33181 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
    33241 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
    33301 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
    33361 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
    33421 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
    33481 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
    33541 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
    33601 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
    33661 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
    33721 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
    33781 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
    33841 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
    33901 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagt
//
LOCUS       DAFBYI010000017        33706 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFBYI010000017 DAFBYI010000000
VERSION     DAFBYI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.017"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            4..816
                     /locus_tag="LLD04_000697"
     CDS             4..816
                     /locus_tag="LLD04_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK5647649.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LLD04_000698"
     CDS             813..1613
                     /locus_tag="LLD04_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK5647650.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LLD04_000699"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LLD04_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK5647651.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LLD04_000700"
     CDS             3110..4819
                     /locus_tag="LLD04_000700"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK5647652.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LLD04_000701"
     CDS             5031..9383
                     /locus_tag="LLD04_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK5647653.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LLD04_000702"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LLD04_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK5647654.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LLD04_000703"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LLD04_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK5647655.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LLD04_000704"
     CDS             11221..11514
                     /locus_tag="LLD04_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK5647656.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LLD04_000705"
     CDS             11511..11822
                     /locus_tag="LLD04_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK5647657.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LLD04_000706"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LLD04_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK5647658.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LLD04_000707"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LLD04_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK5647659.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LLD04_000708"
     CDS             complement(14623..14844)
                     /locus_tag="LLD04_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647660.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="LLD04_000709"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="LLD04_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="LLD04_000710"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="LLD04_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK5647661.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="LLD04_000711"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="LLD04_000711"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK5647662.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="LLD04_000712"
     CDS             20242..21027
                     /locus_tag="LLD04_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5647663.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="LLD04_000713"
     CDS             21040..21834
                     /locus_tag="LLD04_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK5647664.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="LLD04_000714"
     CDS             complement(22014..22466)
                     /locus_tag="LLD04_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647665.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="LLD04_000715"
     CDS             complement(22931..23299)
                     /locus_tag="LLD04_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK5647666.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="LLD04_000716"
     CDS             23449..23931
                     /locus_tag="LLD04_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK5647667.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="LLD04_000717"
     CDS             23968..24978
                     /locus_tag="LLD04_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK5647668.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="LLD04_000718"
     CDS             complement(25093..25806)
                     /locus_tag="LLD04_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647669.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="LLD04_000719"
     CDS             complement(25996..26586)
                     /locus_tag="LLD04_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK5647670.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="LLD04_000720"
     CDS             27070..27297
                     /locus_tag="LLD04_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK5647671.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="LLD04_000721"
     CDS             27313..28479
                     /locus_tag="LLD04_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK5647672.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="LLD04_000722"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="LLD04_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK5647673.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="LLD04_000723"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="LLD04_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK5647674.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="LLD04_000724"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="LLD04_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK5647675.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="LLD04_000725"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="LLD04_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK5647676.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11454 a         5812 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaa
//
LOCUS       DAFBYI010000018        31989 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFBYI010000018 DAFBYI010000000
VERSION     DAFBYI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.018"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(48..1112)
                     /locus_tag="LLD04_000726"
     CDS             complement(48..1112)
                     /locus_tag="LLD04_000726"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK5647677.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="LLD04_000727"
     CDS             complement(1109..2194)
                     /locus_tag="LLD04_000727"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK5647678.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="LLD04_000728"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="LLD04_000728"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK5647679.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="LLD04_000729"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="LLD04_000729"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK5647680.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4437..5096
                     /locus_tag="LLD04_000730"
     CDS             4437..5096
                     /locus_tag="LLD04_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5647681.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            5181..6086
                     /locus_tag="LLD04_000731"
     CDS             5181..6086
                     /locus_tag="LLD04_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5647682.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="LLD04_000732"
     CDS             complement(6088..6720)
                     /locus_tag="LLD04_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5647683.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    6871..6996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="LLD04_000733"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="LLD04_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK5647684.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="LLD04_000734"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="LLD04_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK5647685.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="LLD04_000735"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="LLD04_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK5647686.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="LLD04_000736"
     CDS             8600..9838
                     /locus_tag="LLD04_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK5647687.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="LLD04_000737"
     CDS             9886..12015
                     /locus_tag="LLD04_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK5647688.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="LLD04_000738"
     CDS             11990..12679
                     /locus_tag="LLD04_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647689.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="LLD04_000739"
     CDS             complement(13087..13773)
                     /locus_tag="LLD04_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5647690.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="LLD04_000740"
     CDS             complement(13909..14103)
                     /locus_tag="LLD04_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647691.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14589)
                     /locus_tag="LLD04_000741"
                     /pseudo
     CDS             complement(14153..14589)
                     /locus_tag="LLD04_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(14593..15438)
                     /locus_tag="LLD04_000742"
     CDS             complement(14593..15438)
                     /locus_tag="LLD04_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK5647692.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15587..17005
                     /gene="pepV"
                     /locus_tag="LLD04_000743"
     CDS             15587..17005
                     /gene="pepV"
                     /locus_tag="LLD04_000743"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK5647693.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17081..18283)
                     /locus_tag="LLD04_000744"
     CDS             complement(17081..18283)
                     /locus_tag="LLD04_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647694.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18270..20057)
                     /locus_tag="LLD04_000745"
     CDS             complement(18270..20057)
                     /locus_tag="LLD04_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647695.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20070..21023)
                     /locus_tag="LLD04_000746"
     CDS             complement(20070..21023)
                     /locus_tag="LLD04_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5647696.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21208..21558)
                     /locus_tag="LLD04_000747"
     CDS             complement(21208..21558)
                     /locus_tag="LLD04_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5647697.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21758..22837
                     /gene="pepA"
                     /locus_tag="LLD04_000748"
     CDS             21758..22837
                     /gene="pepA"
                     /locus_tag="LLD04_000748"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK5647698.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22980..23300
                     /locus_tag="LLD04_000749"
     CDS             22980..23300
                     /locus_tag="LLD04_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5647699.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23322..23786
                     /locus_tag="LLD04_000750"
     CDS             23322..23786
                     /locus_tag="LLD04_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5647700.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23982..24587
                     /locus_tag="LLD04_000751"
     CDS             23982..24587
                     /locus_tag="LLD04_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK5647701.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24626..25915)
                     /locus_tag="LLD04_000752"
     CDS             complement(24626..25915)
                     /locus_tag="LLD04_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK5647702.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26343..26822
                     /gene="rlmH"
                     /locus_tag="LLD04_000753"
     CDS             26343..26822
                     /gene="rlmH"
                     /locus_tag="LLD04_000753"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK5647703.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27175..27489
                     /locus_tag="LLD04_000754"
     CDS             27175..27489
                     /locus_tag="LLD04_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5647704.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27505..27891
                     /locus_tag="LLD04_000755"
     CDS             27505..27891
                     /locus_tag="LLD04_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5647705.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27920..29047
                     /locus_tag="LLD04_000756"
                     /pseudo
     CDS             27920..29047
                     /locus_tag="LLD04_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            29780..31639
                     /gene="ltrA"
                     /locus_tag="LLD04_000757"
     CDS             29780..31639
                     /gene="ltrA"
                     /locus_tag="LLD04_000757"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5647706.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            31733..31972
                     /locus_tag="LLD04_000758"
     CDS             31733..31972
                     /locus_tag="LLD04_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK5647707.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
BASE COUNT        10307 a         5911 c         6456 g         9315 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tgctcacttt ttctccgtaa tggatcattt ctttatagct
    14401 gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa
    14461 ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg
    14521 atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa
    14581 tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt
    14641 cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc
    14701 tccaatgaaa ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg
    14761 ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc
    14821 agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact
    14881 tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg
    14941 ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt
    15001 ttgttcggga tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc
    15061 aatcaccagc cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact
    15121 cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga
    15181 aagaaactgg tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag
    15241 agcactccga tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc
    15301 agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat
    15361 acgtccaaat gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag
    15421 ttgttcgttt atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta
    15481 gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt
    15541 gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg
    15601 gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg
    15661 tgtcaacagc gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc
    15721 tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa
    15781 tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt
    15841 tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt
    15901 aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc
    15961 agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg
    16021 cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca
    16081 tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga
    16141 aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt
    16201 gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact
    16261 tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga
    16321 agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt
    16381 tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc
    16441 attgttctta gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc
    16501 tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa
    16561 aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga
    16621 agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca
    16681 aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt
    16741 aatggaacca cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta
    16801 tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg
    16861 tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca
    16921 tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga
    16981 tgccatctat cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg
    17041 gagtttttat gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt
    17101 cttttgattt ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga
    17161 tgatttgcgt acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt
    17221 accaaatata cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc
    17281 agctatttcg tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata
    17341 atattgctac ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca
    17401 acgggtattt gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa
    17461 tgtttttgtt caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc
    17521 ctaatcccca aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc
    17581 gtttatccat taaatactta tttgttacat aataaataat gatcacaggt atcccccaag
    17641 agatgaccgg tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat
    17701 acaagaataa ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag
    17761 acaataaagt caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat
    17821 ttcctatagc aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta
    17881 aaaagtagct cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac
    17941 tccccaaact catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac
    18001 ggggataatc cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg
    18061 tttttcgtat catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt
    18121 gattgataga aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg
    18181 attcaataga gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga
    18241 tcaagagaac aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct
    18301 gttttcattt tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct
    18361 aatgctaaaa caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta
    18421 gcaaaaacaa gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt
    18481 gctaaaagtg cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga
    18541 gcatcgatga tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc
    18601 ttcataccat aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta
    18661 ccaggtaccc agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta
    18721 cttggtaaat ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca
    18781 ttgttaaaat ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa
    18841 tcttttatcg ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc
    18901 ataacaatag ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta
    18961 ggaatagcat aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc
    19021 agcaccactt gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc
    19081 aaacctgtgc cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca
    19141 aggatgatta cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca
    19201 aaaaccgcta aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc
    19261 aaggcgtaga ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga
    19321 aaaaaatgga tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt
    19381 atcaatgttt gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa
    19441 tcatgaacaa atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga
    19501 acatcaccca tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac
    19561 aatgccaaca gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat
    19621 tttcttgaag agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt
    19681 gataaataaa tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga
    19741 aagtaaaaaa tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga
    19801 aattgtatct gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg
    19861 gcaatagctg caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca
    19921 agtagcaaaa ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa
    19981 gaagtaaaaa aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc
    20041 tcctttttct tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg
    20101 tagacatggt ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa
    20161 atcaaccctg ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca
    20221 gaagggatgc gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg
    20281 accaatccaa ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa
    20341 tctttgcaca ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat
    20401 tttgattcgc taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat
    20461 tttttatcta aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata
    20521 gggaagcttt tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt
    20581 tttgttttat ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa
    20641 ggacgcttat tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct
    20701 gcttcgccag aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca
    20761 tcaccatcta ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca
    20821 ttcccttttc cttgacgtgg ttctttcttt acgattgctc cgccttgaac ggccaattcg
    20881 tatgttcgat cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt
    20941 ttaaaatctt caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg
    21001 gcaatttttt cacgtgattc catttgtcac tctccttata taataaaact aatcttattt
    21061 caataatacc gtataataac tacaaatact agctattcca agaggaaata gccctatttt
    21121 tataaaaatc acccactata aaagaaggat aggatacctc tcttttagat agtgggtgcg
    21181 tattcgttat ttcatttttt tgacgtatta atctattgcg cccttttttc ttgaaatgtc
    21241 taatacggtt cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc
    21301 tatccgggaa agcccacccc gataagcact cgaccgaacg gcgaatttcc gaataggttc
    21361 tttttcaaaa gagatccaag caccttcgat tgcgccttct tttaaaaaag atgttttgat
    21421 attttcaagc acttggtctg ctgataacac ttttttgcga tgataccaaa tcgttgaagc
    21481 taccccactc aataatccta gaccgatccc aacggctaac ccgcctttaa aatagcttaa
    21541 ttcgctttct tcatacatgt ggatatacct ccttcttctc acttgctact ctttgtgttt
    21601 attttaccac actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct
    21661 ataataaata cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc
    21721 tgagaggagt ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg
    21781 aatcaaagag ttaacagaat tacaaggaac aagcggtttt gaacaagacg ttcgcgccta
    21841 tatggaaagt catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat
    21901 ttttgggctg cgccaccatg aagaagcgga tgcacctcgt gtaatggtcg cagcacatat
    21961 ggatgaagtc ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc
    22021 tctaggcgga tggaaccctt acgttgtttc tgctcaacgt tttacactta aaacctctac
    22081 tggtaagaat tatccatgta tttcttcttc cgttccccca catttattac gaggaacaag
    22141 cggacaaaaa tcagtagaag taacagatat tttgttcgat gctgggtttg aatcaagaga
    22201 agaggcaatg agttacggtg ttcttccagg agacacaatc gttccttacg ctgaaacgat
    22261 caaaacagcc aacgggaaga atatcatcag caaatcgtgg gacaaccgct atggctgtac
    22321 aatggtatta gaagctttag aagcgttaca aaatgaaaca ttaggtcata cattgattgc
    22381 aggcgcgaat gtccaagaag aagtaggctt gcgtggttct aaagcttctg tcaataaatt
    22441 caagccagat ctgttctttg cagtagactg ctctgcagca gatgacacag tgacgaaaaa
    22501 tggcacattc ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat
    22561 catgcttcct cgtttgcgtg agtacttgct agatatcgct gaaacaaaca acatccctta
    22621 tcaatacttt gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaatgaagg
    22681 aatcccaagt acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt
    22741 cagtattcgc gattttgaag ctgcacgaga aatgttgatt caaacattga aaggtttaga
    22801 taaatcaaca gtgaacacga tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc
    22861 tggcaaaagc aaccttttgt cagatttttt ttataaaaag aggatcaatg tttgttagtt
    22921 cgaaagaaag gtgtataatt taattgaaaa atacataaac agaaagtaga ggaaaaagaa
    22981 tgattatccc aaatagttta gaagaattag caggctacgt agaaactgga aaaagtgtat
    23041 ttttctttac tgccggctgg tgcggagact gccgttttat caaaccgcaa atgcctgaaa
    23101 tcgaaaatga cttttctgat tggcaattta ttgaagtaga ccgtgacaag tacatagatg
    23161 tagccgctga atggaacatt tttggtatcc caagttttgt cgtgatccaa gacggaaaag
    23221 aattaggtcg tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag
    23281 aaagtgtaat aaaaggctga catctgttag gaggggtcaa gatgaatcaa gaatataaga
    23341 acattttagt aggtattgac ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag
    23401 aagtggctcg ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt
    23461 ataattttat gggctatgct cctattgatc aaaatctagt cgatcagcaa actgaaacag
    23521 ccaagcaaat gatcgaagaa tgtaaagaat atggaaaatc tgttgattac acaaatatag
    23581 aaggaattat tgcatacggc tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat
    23641 atgcaatcga tctaatcatg gttgggcagt ctggactaaa tgcagtcgaa cgctttatga
    23701 caggaagtgt agccagctac gtcatacgtc attcaccttg tgatgtcttg atcgtttctg
    23761 acgaagaaga acagccaagt aattaagcaa gtggaacttg tataaccttt ttaatcaaac
    23821 agtaaaggga ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc
    23881 agaagaagct tcttcggaaa taaagcatga aaaaaatctc atgcagtgaa tgcaattttt
    23941 tgttaagata aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca
    24001 aagcacacgt cggagacact ttgatggtta tcatagccga cgataaagga acagaaaaca
    24061 ctgtcgaacg taaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt
    24121 ggaacttttt ccatatctcc gatcatctaa caattgaagg gaacggccaa gtaagcttaa
    24181 cagatgaaga attagcgaaa ttgaatcgat tgattaaaga agcaggattc gaagaacagc
    24241 ttgaagctga tcatgaaccg aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc
    24301 ctgactctga ccatttatcg attactgaga cagaagtgga caatgggaca gtattgcaaa
    24361 ttgtttgcgg tgcaccaaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag
    24421 ccatgatgcc agatggaatg atgatctggc caggaacttt gcgaggggta gccagctatg
    24481 gaatgatttg ttctgctcgt gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct
    24541 tagtattgcc tgaggatgcc gtagtcggag aagtatttcc aaaataaaga ggttgtgaca
    24601 agtctttgtc acaacctctt tattattaat tttcactatt ttgattttgt tttaatgccg
    24661 attgatcgat cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga
    24721 ttttgacaga atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag
    24781 aaacttcttt gtcatcgatc ttagtaatga cgtcgtattg tttcaaccca gctttttctg
    24841 ccggtgtagc tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct
    24901 tcaagatttg ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta
    24961 gagctggacg tgtcactttt ccgtcttttt ccagttggtt gatgatattc actacatcgt
    25021 tacttgggat ggcaaatccc attccttcta cgcttacatt agaagtcgat tcagaagtac
    25081 ttgcgatctt acttgagttg ataccgataa cttgtccttc gatattcact aaaggaccac
    25141 cagaatttcc cgggttgatc gctgcatctg tttggatcgc attgatattg actgtttcgt
    25201 tcgattcatt cgtacttgtt acttgtcggt tcaaggaaga aatgattcct gaagtgacag
    25261 aattcgcata ttcagatcct aatggggaac caatcgcaat cgcaggttca ccaaccttca
    25321 aagcacttga gtccccaaaa gaggcaaccg tttcgacttt gtctgaattg atcttcaaca
    25381 ccgctaaatc tgaataggca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt
    25441 tcatcatgac ttccaaacct tgctgtccat ctactacgtg attgtttgtg actacataag
    25501 cagtattccc gtcttttttg tagattacgc cgcttccttc actataagct tccaaattac
    25561 tatcatctga gctgctactt tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt
    25621 ggttttgact ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt
    25681 tggtgatatc agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat
    25741 tttgattgcc gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa
    25801 tgccagctgt cagtagtcct ccaacgatcc ctccaacaag ccccagaccc aacttacgcc
    25861 agaggctatt atttttattt tttttcatct ttggtgtcac attttttcta tccatgtgtt
    25921 cctcctccaa ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc
    25981 tactttgaga ctttgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga
    26041 aaatatttga ttttcttttt ccacagatat tgtgtaaaac tacgcggata ggattggaaa
    26101 aataagggat aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt
    26161 atgtttgcct aatgaatgtg atgaaataag gtaaaacaac gctgttttct gtggataaac
    26221 ctgtggatgt tgtgcataac tgttggggaa atctttttaa caaaaatagt caaaataaac
    26281 atatagacga aaaaattgat tataataaac aaagtgagcg tgaagatcaa aggagagaaa
    26341 acatgaatat caaaattatt tcagtaggaa aattaaaaga aaaatacttg atccaaggta
    26401 tcaatgagta tgtcaaacgg ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg
    26461 acgaaaaagc cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag
    26521 gagaacggat cttagcgaaa atcaaagaac aagaatacgt ctatggctta gcaattgaag
    26581 ggaaaaatcc cacaagcgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa
    26641 aaagccacct tgtttttgtt atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa
    26701 gaagcaatgc tcagatctct tttggcaaaa tgacgtatcc acatcaattg atgcgcctga
    26761 tcctcgtcga gcaaatctac cgtgccttcc ggatcaacgc gggggctcca taccacaaat
    26821 aatgatattt tttaagagaa aaatagcata aaaatctagt tatccgcata aaaactggac
    26881 ttatcacact ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat
    26941 gagctttgat acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa
    27001 tttgaatatt taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt
    27061 tgaaaggaag tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct
    27121 ttaagaccat caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa
    27181 cttaaatttg tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag
    27241 gataaagtcg ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat
    27301 ctctattctg atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc
    27361 gaaaaacatt tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag
    27421 ggctacaaaa tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata
    27481 aaagaataaa gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa
    27541 gacacgactt ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat
    27601 gaagacgggt cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga
    27661 caaggacgca tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat
    27721 aacgcacggg tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa
    27781 ggagcagatg ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt
    27841 tcattcaaag ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt
    27901 agaaaggaga ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga
    27961 tttagtcttt cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact
    28021 gtttcatgtg aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga
    28081 taagattgac attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct
    28141 ggtagcgttt gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca
    28201 aaaactggca aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga
    28261 gggtttcttt aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat
    28321 gtattatcga cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata
    28381 tcaagaccaa ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac
    28441 ggataaagag ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag
    28501 tcgtatttct attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt
    28561 atggtgggaa tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa
    28621 aacttacttt atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat
    28681 tcttgacccg aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta
    28741 tagaaaagaa gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag
    28801 tgaggaaatg aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct
    28861 cccggcacac ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa
    28921 agaaaacacc gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg
    28981 cttctttcta atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg
    29041 tgatcagtgt gcgacatgtt tctaactgaa aaggttaggc acttaaaaga agaaaacata
    29101 agtttccttt taaggagaac tgatattttg agcagtggaa tgatggggta acgccctgaa
    29161 acgctccctt aatactccga ctggctgttt aacatatagc tgttagatgg tcagaagctc
    29221 ggtgaagtcg gctgaaagtt accgtaaata tgataaattc atatacgaaa gttgttcaga
    29281 ggtgaatgat gtaacctata ggtcggaggt ctataaaata ctcacggtga gaatgtaagt
    29341 aatactactg acgaacttgc gaatgaaaga atctatacac ggactgtata gaaatgtaca
    29401 ggattctacc gtatcaacgg aacagaatgt atgttgtggt ttagtaagat tattggttat
    29461 gaacaaccat gcaatgttac aggcattgga aatatacagg ttcaaagcaa gcacctaaaa
    29521 gtatatataa agataaaaat atcgaaacat gggaagctgg caatgtggag gaaactatat
    29581 tccattgaaa catttgtgac gaagaataat aagcacattt atgccagtga aagtggtggc
    29641 acagtaccaa tgaggatata gaaatatatc ggaggaatag ccactagtca aaagaaatcg
    29701 ttaattaaat gatgtatttc atagaatcga gtaagacaaa ataaacacta caacctaagt
    29761 aaacaaaggt agtggtgcta tgaataaagt aaaaacaaat aaaacacttc gtcatagtga
    29821 atactatggc atgacacaaa tatttgatga actgtatgta aaaagtgata aaaaagaagt
    29881 ttttacagag ctcatgacaa ttattatgag tgatgaaaat attcgactag catacagaaa
    29941 tatcaagaat aacaagggca gttatacagc gagtaccgat aagaaaagta ttcgagacat
    30001 taaaacactg tcagaagata cctttctaac tgaggttaaa gttctttttc gctgttacaa
    30061 accacgagca gttagacgtg tagaaattcc caaaccaaat ggaaaaacac gccctctggg
    30121 cattccatca atctgggata ggttaataca acagtgtatt ttacaagtct tagaaccgat
    30181 atgcgaagct aaatttcaca attatagtta tggatttcga ccaaaccgtt ccacacatca
    30241 tgcgattgct acctgcaatc gactggcaaa tagggggaag ctacactttg tagtagatgt
    30301 tgatattaaa ggattctttg ataatgtcaa tcactctaaa cttttgcgtc aaatgtggac
    30361 gattggaatc cgtgataaat ggctattagg tattattcga tccatgctaa atgctcctat
    30421 agtcctacca accggtaaag tcgaacaccc taaaaaaggt gtacctcaag gtggtgtcct
    30481 atccccttta ctatctaata tcatattgaa tgaattagat tggtgggttg cttcacaatg
    30541 gcaaaatacc acgattgaga cagaaaagat tatggatagg cgagataaag gcaaaggcat
    30601 tgataagact aatcagtatc gggcattaag acgaacaaac ttaaaagaaa tatacattgt
    30661 tcggtatgct gacgacttca aaatcttttg tagaaaacga agtcatgcag ataaaatatt
    30721 tcacgcaacg aaaatgtggc ttaaagaacg gttatcactt gaaattagcg aagaaaaatc
    30781 caaaattgtc aatctcaaaa aaggtaaatc agaatatcta ggatttgaat taaagctaat
    30841 caagaaaggt aaaaaatacg tagtcgaaag tcatatgagc aaaaaatcaa tgacaagagt
    30901 taaaatccaa ttaaagaaac aattacgtaa agttgccaga ccgaagaatc attgcgaaca
    30961 agcaaaagaa attggattat ataattctat ggtgattgga atccatcaat attatggaat
    31021 tgcaacctgt gtaaatctcg attgcagtaa aatagcctat acactaaaac cgttcatcaa
    31081 tcacaaactg ccgacaaaga aacatggaaa gatattaaat acatttatca aatcgaagta
    31141 tggaaaaagt aaagaaatgc gatggttgaa taatgtgcca attgtaccgc tcaactatgt
    31201 tcagtttaga cttgctgtcc ccctatcaga gggaacttgc aagtacactg aaagtggacg
    31261 aagcaagata cacagtaaat taaaattaga cctagccctt ttacttcaca tgatgagaaa
    31321 tagtaattat gaaagaagta ttgaatttgt ggataacaga atatctaaat attctgctca
    31381 aaagggaaaa tgtgctatca cgggtaaatt tctcgaatac gaagaaatcc attgccacca
    31441 tatcgtacca gtaaaacaac agggtacaga caaatacagc aacctcatta ttattcacaa
    31501 aaatgttcac gcattagtcc atgctatcga agaaaaaatc atacacaaat acctgaatgt
    31561 attaaatcta accaatgaac aaatagaaaa actaaatgaa ttacgtgtca aagctggtaa
    31621 tagtgtttta acagtttaac ttgaaaaata tatcatactt aacattcttt tgatggaacg
    31681 ccgtatgaag tgaaagtttc atgtacggtg tgaagcgggg gaaaatctgg agatgacatc
    31741 aaaggattac ctatcgctat tcaactttcg cgtggctttg ggtcgtatga gtgaactcgg
    31801 atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca tcaaaggtcg
    31861 aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac ttgtaccaaa
    31921 agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt gagtgatctt
    31981 caatactga
//
LOCUS       DAFBYI010000019        31087 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFBYI010000019 DAFBYI010000000
VERSION     DAFBYI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.019"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(23..2095)
                     /locus_tag="LLD04_000759"
     CDS             complement(23..2095)
                     /locus_tag="LLD04_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5647708.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="LLD04_000760"
     CDS             complement(2028..2405)
                     /locus_tag="LLD04_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK5647709.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3112)
                     /locus_tag="LLD04_000761"
     CDS             complement(2534..3112)
                     /locus_tag="LLD04_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5647710.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="LLD04_000762"
     CDS             3244..4536
                     /locus_tag="LLD04_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5647711.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="LLD04_000763"
     CDS             complement(4596..5714)
                     /locus_tag="LLD04_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK5647712.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="LLD04_000764"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="LLD04_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK5647713.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="LLD04_000765"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="LLD04_000765"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK5647714.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="LLD04_000766"
     CDS             8920..9519
                     /locus_tag="LLD04_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK5647715.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="LLD04_000767"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="LLD04_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK5647716.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="LLD04_000768"
     CDS             complement(11214..11558)
                     /locus_tag="LLD04_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK5647717.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12840)
                     /locus_tag="LLD04_000769"
     CDS             complement(11695..12840)
                     /locus_tag="LLD04_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5647718.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="LLD04_000770"
     CDS             complement(13020..13994)
                     /locus_tag="LLD04_000770"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5647719.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="LLD04_000771"
     CDS             complement(14074..14949)
                     /locus_tag="LLD04_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK5647720.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="LLD04_000772"
     CDS             15079..16986
                     /locus_tag="LLD04_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK5647721.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="LLD04_000773"
     CDS             complement(17527..20805)
                     /locus_tag="LLD04_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK5647722.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="LLD04_000774"
     CDS             complement(21076..21621)
                     /locus_tag="LLD04_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5647723.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="LLD04_000775"
     CDS             complement(21633..22325)
                     /locus_tag="LLD04_000775"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK5647724.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="LLD04_000776"
     CDS             complement(22344..22628)
                     /locus_tag="LLD04_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647725.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="LLD04_000777"
     CDS             complement(22641..23204)
                     /locus_tag="LLD04_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5647726.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="LLD04_000778"
     CDS             23432..24094
                     /locus_tag="LLD04_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK5647727.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="LLD04_000779"
     CDS             24218..25414
                     /locus_tag="LLD04_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK5647728.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="LLD04_000780"
     CDS             complement(25515..26891)
                     /locus_tag="LLD04_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647729.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="LLD04_000781"
     CDS             complement(26928..27794)
                     /locus_tag="LLD04_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK5647730.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="LLD04_000782"
     CDS             complement(27883..28908)
                     /locus_tag="LLD04_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5647731.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="LLD04_000783"
     CDS             complement(28926..29600)
                     /locus_tag="LLD04_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5647732.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="LLD04_000784"
     CDS             complement(29784..30677)
                     /locus_tag="LLD04_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5647733.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..>31087)
                     /locus_tag="LLD04_000785"
     CDS             complement(30751..>31087)
                     /locus_tag="LLD04_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5647734.1"
                     /translation="TEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEF
                     IISYGSYNEEIQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWY
                     RKSKMKSEV"
BASE COUNT         8975 a         6659 c         5557 g         9896 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta tcattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttccttttct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgtt
//
LOCUS       DAFBYI010000020        30466 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFBYI010000020 DAFBYI010000000
VERSION     DAFBYI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.020"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..552)
                     /locus_tag="LLD04_000786"
     CDS             complement(<1..552)
                     /locus_tag="LLD04_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK5647735.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="LLD04_000787"
     CDS             complement(678..2606)
                     /locus_tag="LLD04_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK5647736.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="LLD04_000788"
     CDS             complement(2912..3628)
                     /locus_tag="LLD04_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5647737.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LLD04_000789"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LLD04_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK5647738.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LLD04_000790"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LLD04_000790"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK5647739.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="LLD04_000791"
     CDS             complement(7098..8558)
                     /locus_tag="LLD04_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647740.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="LLD04_000792"
     CDS             complement(8548..9843)
                     /locus_tag="LLD04_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5647741.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="LLD04_000793"
     CDS             complement(10175..10852)
                     /locus_tag="LLD04_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK5647742.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="LLD04_000794"
     CDS             complement(10882..11973)
                     /locus_tag="LLD04_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK5647743.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="LLD04_000795"
     CDS             complement(12074..12508)
                     /locus_tag="LLD04_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5647744.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LLD04_000796"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LLD04_000796"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5647745.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="LLD04_000797"
     CDS             complement(14546..16441)
                     /locus_tag="LLD04_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5647746.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="LLD04_000798"
     CDS             complement(16499..18274)
                     /locus_tag="LLD04_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5647747.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="LLD04_000799"
     CDS             complement(18324..19820)
                     /locus_tag="LLD04_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5647748.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="LLD04_000800"
     CDS             complement(20014..21636)
                     /locus_tag="LLD04_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5647749.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="LLD04_000801"
     CDS             complement(21964..23277)
                     /locus_tag="LLD04_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5647750.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="LLD04_000802"
     CDS             complement(23522..23746)
                     /locus_tag="LLD04_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647751.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24188)
                     /locus_tag="LLD04_000803"
     CDS             complement(23766..24188)
                     /locus_tag="LLD04_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK5647752.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="LLD04_000804"
     CDS             complement(24330..25040)
                     /locus_tag="LLD04_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647753.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="LLD04_000805"
     CDS             complement(25058..25555)
                     /locus_tag="LLD04_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647754.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="LLD04_000806"
     CDS             complement(25592..26044)
                     /locus_tag="LLD04_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5647755.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="LLD04_000807"
     CDS             complement(26179..26784)
                     /locus_tag="LLD04_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5647756.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="LLD04_000808"
     CDS             complement(27234..27854)
                     /locus_tag="LLD04_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5647757.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="LLD04_000809"
     CDS             complement(27856..28725)
                     /locus_tag="LLD04_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK5647758.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="LLD04_000810"
     CDS             complement(28748..29155)
                     /locus_tag="LLD04_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5647759.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="LLD04_000811"
     CDS             complement(29174..30463)
                     /locus_tag="LLD04_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5647760.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8629 a         6627 c         5023 g        10187 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttgt tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc catgtt
//
LOCUS       DAFBYI010000021        29721 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFBYI010000021 DAFBYI010000000
VERSION     DAFBYI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.021"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(66..872)
                     /gene="proC"
                     /locus_tag="LLD04_000812"
     CDS             complement(66..872)
                     /gene="proC"
                     /locus_tag="LLD04_000812"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK5647761.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            1013..3586
                     /gene="mprF"
                     /locus_tag="LLD04_000813"
     CDS             1013..3586
                     /gene="mprF"
                     /locus_tag="LLD04_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5647762.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3728..4591
                     /locus_tag="LLD04_000814"
     CDS             3728..4591
                     /locus_tag="LLD04_000814"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK5647763.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            4836..5774
                     /locus_tag="LLD04_000815"
     CDS             4836..5774
                     /locus_tag="LLD04_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK5647764.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            5797..6477
                     /locus_tag="LLD04_000816"
     CDS             5797..6477
                     /locus_tag="LLD04_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5647765.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            6450..7274
                     /locus_tag="LLD04_000817"
     CDS             6450..7274
                     /locus_tag="LLD04_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5647766.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            7441..8259
                     /gene="purR"
                     /locus_tag="LLD04_000818"
     CDS             7441..8259
                     /gene="purR"
                     /locus_tag="LLD04_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK5647767.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            8762..10135
                     /gene="glmU"
                     /locus_tag="LLD04_000819"
     CDS             8762..10135
                     /gene="glmU"
                     /locus_tag="LLD04_000819"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK5647768.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            10229..11200
                     /locus_tag="LLD04_000820"
     CDS             10229..11200
                     /locus_tag="LLD04_000820"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5647769.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      11206..11308
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11374..11943
                     /gene="thiT"
                     /locus_tag="LLD04_000821"
     CDS             11374..11943
                     /gene="thiT"
                     /locus_tag="LLD04_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK5647770.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(12024..12797)
                     /locus_tag="LLD04_000822"
     CDS             complement(12024..12797)
                     /locus_tag="LLD04_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647771.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            13063..14763
                     /locus_tag="LLD04_000823"
     CDS             13063..14763
                     /locus_tag="LLD04_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK5647772.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            14733..14951
                     /locus_tag="LLD04_000824"
     CDS             14733..14951
                     /locus_tag="LLD04_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK5647773.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            14971..15861
                     /locus_tag="LLD04_000825"
     CDS             14971..15861
                     /locus_tag="LLD04_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK5647774.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKTREQVQNIE"
     gene            15879..16163
                     /locus_tag="LLD04_000826"
     CDS             15879..16163
                     /locus_tag="LLD04_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647775.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            16650..17264
                     /gene="gmk"
                     /locus_tag="LLD04_000827"
     CDS             16650..17264
                     /gene="gmk"
                     /locus_tag="LLD04_000827"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK5647776.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            17269..17574
                     /locus_tag="LLD04_000828"
     CDS             17269..17574
                     /locus_tag="LLD04_000828"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK5647777.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            17827..20235
                     /gene="priA"
                     /locus_tag="LLD04_000829"
     CDS             17827..20235
                     /gene="priA"
                     /locus_tag="LLD04_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK5647778.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            20250..20741
                     /gene="def"
                     /locus_tag="LLD04_000830"
     CDS             20250..20741
                     /gene="def"
                     /locus_tag="LLD04_000830"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5647779.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            20734..21672
                     /locus_tag="LLD04_000831"
     CDS             20734..21672
                     /locus_tag="LLD04_000831"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK5647780.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            21672..23030
                     /gene="rsmB"
                     /locus_tag="LLD04_000832"
     CDS             21672..23030
                     /gene="rsmB"
                     /locus_tag="LLD04_000832"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK5647781.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            23043..23783
                     /locus_tag="LLD04_000833"
     CDS             23043..23783
                     /locus_tag="LLD04_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK5647782.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            23780..25849
                     /gene="pknB"
                     /locus_tag="LLD04_000834"
     CDS             23780..25849
                     /gene="pknB"
                     /locus_tag="LLD04_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK5647783.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            26014..26913
                     /gene="rsgA"
                     /locus_tag="LLD04_000835"
     CDS             26014..26913
                     /gene="rsgA"
                     /locus_tag="LLD04_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK5647784.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            26926..27576
                     /locus_tag="LLD04_000836"
     CDS             26926..27576
                     /locus_tag="LLD04_000836"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5647785.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            27577..28215
                     /locus_tag="LLD04_000837"
     CDS             27577..28215
                     /locus_tag="LLD04_000837"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK5647786.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            28370..29224
                     /gene="mreC"
                     /locus_tag="LLD04_000838"
     CDS             28370..29224
                     /gene="mreC"
                     /locus_tag="LLD04_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK5647787.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            29228..>29721
                     /gene="mreD"
                     /locus_tag="LLD04_000839"
     CDS             29228..>29721
                     /gene="mreD"
                     /locus_tag="LLD04_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK5647788.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
BASE COUNT         9681 a         5278 c         6454 g         8308 t
ORIGIN      
        1 agaagagccg cggcaaaagc cacagctctt ctttttttgt aaggtacgaa aaaacattgc
       61 tatcattatt ttttattcaa catctcaaga tctttctcga tcgtcgtatt gattcctttg
      121 atgattgctg ggatgaatcc tgcttcttct aaggcaacta cacctgcaac agttgtaccg
      181 ccaggagaag aaacttggtc tacaagttcc catggatttt tatcacttga aaggaatttt
      241 tgacaagaac ccataattgc ttttgcagca atctcagtgg ctgtctgttt ggacaaacca
      301 ttcagtacac cagtccgtcc catcgcatcg atcagcatgt aaacgaatgc gggtgagctg
      361 ccagcaaggc caatgaatgt actgaaatct gattctgcta aaggatagac gctgccgatt
      421 gcttcaaaaa tcatcgtagc ttgctgaagc tgttcctccg tggcagcttc atttttgcaa
      481 atcgcagaaa cgcctgctcc tacgctgaca ttgacattcg gcatcacacg tacgatggag
      541 gcctgaggat cttcaagcgc tgtttctaaa tctgttaatg attttcctga agcaatagaa
      601 atgaataaga ctttctggct atattctctt agttcttcca atactttttc aatgattttt
      661 ggtttcacgg ctaaaagaac gatatctgat tgtgatgcaa cttcctgatt acttttgcat
      721 gcatggacgc cagtttcagc agcaaatttt tgtgcatttt caaaatgtcc actggaaacg
      781 aacacctctt ctttcgagac tgttcctttt tttaataaac catcaataat agcttgtgcc
      841 atattccctg tgccgataaa tcctactttc atgttatcct ctcctttagc attaggatat
      901 ctttcttttt ctatcatcgc aagcaaagaa caggtctaaa gtggaaaaat taaaataaat
      961 aagataacat tggggtatga cacaaaagta tacaagttag gaatgggaaa atgtgaaaaa
     1021 taaacgatac ttgatgctgc aatggataaa ggaacatagt ctgattttaa aactgatttt
     1081 tttcggctcc gtgttagtct ttgtggcaaa tcaagtcgca aatattgcta atggcatgag
     1141 ttggcaagat atttggcaaa ggatgggaca acaaagccgc atgacgatcc tgctcatgat
     1201 tctggctgga ctgctgggtg tcactccgat gttgttgtat gattgggtga cgatcagagt
     1261 cttagaaaaa caaggaaaac caaaaatgga aagaaaagag tttcttcttg ctgcgtgggt
     1321 aacaaacacg atcaacaatc tcgccggttt tggtggtgtg gtaggagcaa gtctgcgggc
     1381 tagtttttat ggaaagcaga caaatagaaa aatggtttta gccactgtat caaaagtcgc
     1441 actatttatg ctgactggtt tgtctgtatg gtcgtttctt acatttattg atgtcttttt
     1501 tattcagtca gaaagtattt ttcgaagcta ttggatctgg cttttcggcg gtagtttttt
     1561 agcaccagtc attttctttt ttgcttatct aaagagaaag acattgttct tcgagtttta
     1621 tccaaaaggc ttgcttggtc tgctactggc atcctttgga caatggacgg gagcccttgc
     1681 ggtatttttg atcatcggca agttgatgag aatagatatt tcgatgcttt cgatctatcc
     1741 tatgttcatg attgctacat tgataggaat gctgacgatg gtcccaggtg gaatggggac
     1801 ttttgacgtt ttgatgatct taggaatgag tcaattgtct gttcggcaag atgtagcagt
     1861 agtttggcta ttatattatc ggttgtttta ttatgttcta ccttttatga cgggacttct
     1921 attgttcatc acacatacag gagtcaaaat caatcgattt ttagataaca ttccgcgact
     1981 gtttttccaa aaggttgcgc atatcgtgct cgtaagtgct gtctattttg cggggatcat
     2041 gatggtactt ctatcaacaa tcactaatct gtctgcaatc agtcggctat ttcaagtatt
     2101 attgccgttc tcattcaatt ttttagatca gacagtgaat ctattggttg gctttttatt
     2161 actcggcctt gctagagcac tttcagtaaa agtcaaaaag gcatttctgc ctacgattgc
     2221 tttactatct tttggtattt taaatacaat cacacgtacg ttatcttggc agttgatatt
     2281 ggtatatgtc ttgattctgt tagctgtgtg gttatccaga aaagaatttt atcgaacaaa
     2341 attcgtttat tcttggggag ctataatctt tgatgctagc ttgttcggct ttttgtttat
     2401 cgtttacgca attgcaggct atcacagcgg acgcttttta gtcggtcgaa taacaggaaa
     2461 tcattttatt ttgtttcctt cagatgatgt gtggttctca gggcttattg gtttgggcat
     2521 ctcattgcta gctttagtgg cgctttatca atatttagct gaagatacta cgaagcttgg
     2581 cagtgagtac gaaaaagacc gttttgaccg tttattggaa acatacggag gcacagctac
     2641 aagccatcag cttcgcttgt caggttacag ttatttttat taccaagaag acgaccaaga
     2701 tcaagtcgtt tttggttatc agatcaaagg aaacaaatgc tttgttttag gagatccagt
     2761 tggcaatcct aaaatgatgc gggcagcaac gaatcagttt atgaaacaag cagattgttt
     2821 aggctatcag cttgcgtttt ataagatatc agaaagctac gtagtgatgc ttcatgaatt
     2881 gggttttcac ttcacgaaag taggtgaagc agggattgtt gatctagcaa cagaagactt
     2941 gtctcggctg agtcagcaac ttgaatatca acgtttgatg aaagaaggat accattttac
     3001 ttggtacgat caactgccgg aagaattaca tcaagctgta aatgaagttt cacaaaaatg
     3061 gcttcaaggt gcacgagaaa aatacttctc tgtgggacga tttgataaat catatgtatt
     3121 atctagcggt gtcggtattg tccgtaaaga tcaaaaagtc gttggcttca tcaccgctca
     3181 gccgatgacg aagaagcagg cgggatatga tttgttgcgg gtcttaccta aagaatctga
     3241 ggaattagct gactatctgt tagccaatct gtttattgta tacgagaaaa tgggctactc
     3301 ggaagccaat ctaggcttag ctccattagc taatgttgga gaaacagatt tttctttctt
     3361 tcaagaaaaa gtcatgcata tcgtatataa ttatggtaat tttttctatt ccttccagtc
     3421 tgcttatgaa aagaaattaa aatatgtcga tcattgggaa ggacgttatt ttgcttatag
     3481 gaaaggaagt aatttcggat ttgctaccat gcagctactc agtctgatag gaaaagaaaa
     3541 aggaaatgtt ccgagtttgg cagaagaagt gctcacagaa ttataagaat cactagaact
     3601 tgtttggatt atgaagagaa aattctgtga aagctgttga aacgatgaca aaatcaacca
     3661 ctaggcagac ataagctata cttttatttt tccctatgat agaatacttg cgacttagac
     3721 ttttcgaatg ggtgagagga tggaattgat tgaaaaagcg ccagccaaga taaatttagg
     3781 actggatgta ttatatagac ggcaagatgg gtatcatgag ttggaaatgg tgatgtcgag
     3841 cgtagacttg gcagatcatc tattttttga agaaataaca gaagacaaaa tcattgtaga
     3901 aacaaataaa gcatttttac cggtagatga acggaacaat gtttatcaag ctgctttatt
     3961 gctaaaaaat aaatacaata tcaaaaccgg tgtcaagatc tttgttaaga agaatattcc
     4021 agtagcagct ggattaggcg gaggaagtac agactgtgca gctgctctaa gagggatcaa
     4081 ccaattatgg aatctgggct gttcgttaga agaactggca gaaatcggtt tggaagtcgg
     4141 tacagatgtt ccttactgtg tctacggaag tacggcattt gtcggtggca aaggggaaga
     4201 aatccaagta ttaccttcta tgccacagtg ctgggttgtg ttggtcaaac caagaatgag
     4261 tgtctctaca cgtacagtgt ttagagaagt agatacgcaa gccatccaac atcaagacat
     4321 ccacggactt gctgaagcca tccgagagca agattatcag cagatgatca aaaaaatggg
     4381 aaacagttta gaagatataa cgggaaagcg gcatccagtc atccatcaaa tcaaagagcg
     4441 tatgatcaag tacggagcag atgctgcatt gatgagtgga agcggaccaa ccgtttttgc
     4501 cttgtgtcaa cattattcaa gagcgcagag agtatataat gggttaaaag gtttttgtga
     4561 ggaagtttat ttagtacgaa ccctgcaata aaatataaga agtaaaatag gtgatgaaaa
     4621 actgaggaat cctaaaatgg tattcttcag ttttttattg tagtgaaatg gctagatatg
     4681 agcagcaact catccatttt acgatttaat caagaatata catgcttaaa tcagaaattg
     4741 atggatttct attgactgtg tttttttatt ttgatacatt agttcacgtt aataaatcgt
     4801 aaaattccga tttgttcatt gaaaggagca tataaatgaa aaaatatctt ttgacagcta
     4861 cggtaatgat aggtgcttta ctgtttgcag catgtggaaa tacgaacaag gaagcagaca
     4921 aaaaagagga tttgacgatc gttacgactt tttatccgat gtatgatttt acaaaagaga
     4981 tcgtagggga tgaaggaaac gtgaaacttt tgatccctgc tggaacggag ccgcatgatt
     5041 tcgaaccaag tgcaaaagaa agagcagaaa tttccgatgc agatgttttc gtgtataata
     5101 gttcagacat ggaatttttt gtggattcgt taaaggattc agtggatagc aaacagacct
     5161 tgatgatcga agcagctaaa ggaatcgacc gtttagaatc gcaagaagca gacgaacatg
     5221 aagaatcaga agaagggcat ggtcattcac atgaatacga tccacatgtt tggctcgatc
     5281 cggtattagc tataaaagaa gtcagaacga ttgctgaaga attaggcgaa aaatatccag
     5341 ataaaaagga aacttttacg aagaatgccg atgcttatat caaaaagctt gaagcactgg
     5401 atcaaaaata ctccgatgag ctaaaagatg caacaaaccg cacatttgtc actcagcatg
     5461 ctgcttttgc ttatttagcg aatcaatacg acttaaaaca agtagcgatt tcgggcgttt
     5521 caccagatca ggaacccaca ccaagtcgct tagctgagct aaaagaattt gtgaagaaaa
     5581 acaacatcaa agtgatttat tttgaagaaa atgcctcttc gaaggtagct gagacattgt
     5641 cgaatgaaac aggagtgaag ctagaagtgt tgaatccttt agaaagcttg actaatgaac
     5701 aaatcaaagc aggcgaaaat tatatttctg tcatggaaaa aaatctgaaa gctttaaaag
     5761 aaagtatcaa ttaagtttta gaatggaggt tgtaaggtgc gttatatcga aatatctgat
     5821 ttgacctttt actatgatga tgaacctgta ctagaagatg tctcttatca tgtagatgcg
     5881 ggtgaatttg tcattttaac tggagaaaat ggagcagcca agtcaacctt ggtgaaagca
     5941 tcgttaggat tactgaaacc ttcaaaagga atgataaaga ttgctgagaa aaatcaagaa
     6001 ggaaaaaaat taagtatcgg ttatattcct caacaagttg catctttcaa tgcaggtttt
     6061 cctagtacgg tgatcgaatt agtccgttca ggaagatatc caagaggccg ctggttcaag
     6121 cgtctctctg aaaaagatca tcaacatgta cagcgtgctt tagaagctgt agggatgtgg
     6181 gatatgcgaa atcggcgaat aggtgaattg tctggcggac aaaagcagcg aatcagttta
     6241 gcgcgtattt ttgctactga ccctgactta tttgttcttg atgaaccaac aacaggtatg
     6301 gatgaagaat ccagaaatga attttatcgt cttctccgac atagtgcgca tgtccatggg
     6361 aaatcagttc taatgatcac ccatgatcat gaagatatca aacagtatgc ggacagacag
     6421 atccgactgg tgagaaagga ggattctcaa tggcgttgct ttcatatgaa ttcatgagaa
     6481 gagcgtttct tgccgctatc ttcattgccg gaatcgcccc gatgctaggt gtgtttttag
     6541 tgatcagaag acaatcgctg atggctgata cgctttctca tgtctcttta gcaggggtag
     6601 cgttaggatt ctttttgaac cttaatccta cacttacgac catgctagtc gttgtattag
     6661 cagctgtgat attggagtat ctgcgaacga tctaccgaac ctattcagaa atctccattg
     6721 ccattttaat ggctggtggt ttggcgctag cgttagtatt gatgaatttg agcggaggga
     6781 attcagcaac aagtatccaa tcctatctgt ttggttcgat cgtgaccatt acatgggagc
     6841 aagtcatctt tttaggaatc ttattcgttg ccatcggtat attgttcttt ttattcaaac
     6901 ggccgatgta tgttctgact tttgatgaag ataccgccca cgtggatggg ctacccgttc
     6961 atttgatgtc gatgatgttc aatgtgatta ctggtgtggc tattgctgtg atgatcccga
     7021 ttgccggtgc acttttgata tccgcaatca tggttcttcc cgcagcaatc ggtatgcgat
     7081 taggcaaaag cttcaatgtc gtgattgcta tcagtgtttg tgtaggattt ctagggatgc
     7141 tgtcaggact aactagctcg ttttatctag atacacctcc aggagcaaca atcacattag
     7201 tctttattgc tttgttcctg attgtaaata tcatcaaacg aatattggtt gccttcagaa
     7261 gaaaaaataa ctaaattata aagaaaagaa gccttataaa aacgaaaaat tttttatagg
     7321 cttcttttta ttttaataaa taccgaacta ttttcttatt ttttcataaa atgctcgaat
     7381 ttttgaagaa aaacttatat aatgtaaaac gataaaattt cttaaaagga gttggaatag
     7441 gtgaaagtac gtcgcagtga acgtctagtt gacatgacac agtatttatt gaatcacccg
     7501 catgaattga tttccttaac aagctttgcc gaacgttatg aatctgctaa atcttctatc
     7561 agtgaagatt tgggaatcat caagaaaaca tttaaagaac gagggtatgg aaccttggaa
     7621 acaatcccgg gtgctgcagg tggtgtattg ttcatccctg aaatttcata tgaagaggcg
     7681 aaaaaatata ttgaatctct agcagaacgt ctatcagaac aagaccgtct gcttccagga
     7741 ggatatgttt atctctctga tttgttagga gaacctgaac tgctgcgtca agttggaaga
     7801 atcattgctt ctaaatacct tggtgaaaaa atcgatgctg taatgactgt agccacaaaa
     7861 ggcgtaccta ttgcccaagc tgtgtcctat tatctgaatg cgccattcgt tattgtacgc
     7921 cgggactcaa aaataacaga aggatcaaca gtaagtgtca actatgtgtc tggatcttct
     7981 gaaagaattg aaaagatgga actgtctaag agaagtttga aacgtggttc acgtgtcctt
     8041 gtagtggatg atttcatgaa aggcggcgga acagtcaacg gaatgaagag cttgattgaa
     8101 gaattcgaat cagaacttgt tggcatcact gtatttgccg aatcaaaatt caacggtcat
     8161 cgcgccatcg atgattatac gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa
     8221 aatattacgg ttgtgccagg aaattatttt tcagaataag aggttgtgaa aaagccgctt
     8281 agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttt cataaatttg
     8341 gcttaatacc gagaaactgg cttttgaaca ccgtttatct agaggttgtg aaatcagcgt
     8401 cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt gccgtttatt
     8461 gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg taagcctacc
     8521 gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt ttatgagtag
     8581 tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga taggcccgtt
     8641 atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca cttgttttgt
     8701 tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag gagtgacgca
     8761 gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca tgaagtcaaa
     8821 attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata tcattaatcg
     8881 ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag cagaacaagt
     8941 taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat tgggaacagg
     9001 acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa cgttagttat
     9061 cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg aatatcacca
     9121 aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa caggatatgg
     9181 acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa aagatgcaac
     9241 accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg acaacgaagc
     9301 tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat actatctaac
     9361 agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc aaacggatga
     9421 ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa atgagatcat
     9481 gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg attcagcaac
     9541 gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag caggtgtaca
     9601 aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt caaaaatcgt
     9661 agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa gcagccatgt
     9721 caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg aaattggcga
     9781 aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca aaggaactaa
     9841 agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca atgtcggatg
     9901 tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg tcggcgacca
     9961 cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta aaaacagttc
    10021 gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg caattgcgag
    10081 agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa actaagactt
    10141 ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt agaattctag
    10201 agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc caagattaaa
    10261 aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag cagtcggcgt
    10321 cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag taaacattga
    10381 agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc cagtcaatga
    10441 taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg caaaaacaat
    10501 caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac gtgcacggga
    10561 accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa caagaatgct
    10621 gactttggac cttcatgcag tccaaatcca aggtttcttt gatattccag tcgatcattt
    10681 gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag gcgatgatgt
    10741 tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag cggaattttt
    10801 gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg cagaagtaat
    10861 gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata tgatcgatac
    10921 agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa ctagtgtgta
    10981 tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag aagattcagc
    11041 tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta aaatcgacaa
    11101 aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta tccatgaaaa
    11161 caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact cacaaagggg
    11221 agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta tcggttatgc
    11281 gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc ttcctccttt
    11341 gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt ttgggtagaa
    11401 ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca gatcggcagc
    11461 agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag aagagggaca
    11521 aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt gggacaagtt
    11581 tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac ttttgcaggc
    11641 gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa tacgaaacaa
    11701 attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata tttttggcat
    11761 tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa ttcttggctc
    11821 ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac gatcatcgtt
    11881 ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg acaggagctg
    11941 taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct tgtttccttt
    12001 ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc ggcatcttct
    12061 accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat ttcctcttct
    12121 cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat tgggatacga
    12181 tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc ctgcatcaaa
    12241 ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc ttttttgaaa
    12301 aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact agcaaaaatc
    12361 ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt aagatcgaag
    12421 tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt tttcatatga
    12481 ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat cgttaaatca
    12541 gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga ggaaaagaaa
    12601 cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc atattctcca
    12661 gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat actgtttgca
    12721 aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc aatatgtaca
    12781 taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt ttattttaca
    12841 acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa aatcacgttt
    12901 tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt ttatttaaag
    12961 gatgttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga ctcatgacct
    13021 ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat aaaaaaagtt
    13081 tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca accccttcca
    13141 ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa aaagcaatgt
    13201 gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa aagtagagga
    13261 gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa aaaatttttt
    13321 cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt tttatttcaa
    13381 tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca aggaaccaca
    13441 caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg aggagcacac
    13501 ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa ctttgagatt
    13561 atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg agatatgaaa
    13621 aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt accagaaatg
    13681 gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat gacgaaaaca
    13741 gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa agacaatttg
    13801 gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc tactaaactg
    13861 ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga aataccagat
    13921 tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat cggaaaaaat
    13981 aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat taacaaagga
    14041 gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa aattcgtatg
    14101 ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag ctttctgctt
    14161 tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat gaaaaaacga
    14221 ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc aagaacatac
    14281 tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc ttttttcaaa
    14341 agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt accagagaca
    14401 tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc caatgaaatc
    14461 tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag tatgccggat
    14521 gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta tcgaaaatac
    14581 cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct gctacaagga
    14641 aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac attactgatc
    14701 ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca tatgaaatcg
    14761 tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa ctgaacctag
    14821 tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca gaccatgaaa
    14881 aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa aaagcaatag
    14941 aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt tggcaaagcc
    15001 atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa ccagcgtttt
    15061 ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga ttttcgtcag
    15121 cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct agcttatatt
    15181 ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact tatcactcaa
    15241 ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat tagtcggatg
    15301 gcggaacgct tcgcgctgga cgaaaacttc gtaacggtag aagaaaaacc ggaagagagc
    15361 atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga ttctattgag
    15421 gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa tactgaagaa
    15481 ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa ggaatttcaa
    15541 ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga gcaacggttg
    15601 ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact ggatcgtctg
    15661 gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt aggtcgggaa
    15721 ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc caaatcaaat
    15781 gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa aaccagagaa
    15841 caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat gacgtaacgt
    15901 tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa aaaaacggct
    15961 ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt attttgatca
    16021 acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac acagaagagg
    16081 tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt gtagtgaata
    16141 ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat aaataatcag
    16201 aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt tgtgacaaaa
    16261 ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat tttctcggta
    16321 ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt cttcatactc
    16381 gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt gcatctgcga
    16441 taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg ttcttattac
    16501 ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt accaatttct
    16561 agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata aacgataagt
    16621 acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta atcgtgttgt
    16681 ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag agtgaggata
    16741 atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg gaggtcgaag
    16801 gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa gctggagaga
    16861 tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca tatgtcaaca
    16921 agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga gcaaaacaag
    16981 tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat ttagcggaat
    17041 taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa gaacgaatga
    17101 aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt gtaaatgatg
    17161 aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat tttcgagtag
    17221 atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat gatgttaaaa
    17281 ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt gattttagca
    17341 agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc ttttgattca
    17401 gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt caatgatcca
    17461 catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca agcacaaaaa
    17521 gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat gtaataaaga
    17581 aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt caaacattcg
    17641 ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt cttttttttc
    17701 ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc catcgatctc
    17761 tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa aaggaggaaa
    17821 cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca gaccgatcag
    17881 ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg gatgcgagta
    17941 gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc tatccaaaaa
    18001 tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga ccttgctccg
    18061 gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac ttacgcattc
    18121 aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata tgataaattg
    18181 atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg agaagtgatt
    18241 tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg gcgccaagag
    18301 caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac gattcgtttg
    18361 gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt acggcaaaat
    18421 gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga agaaccaatt
    18481 gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa agaaaagggc
    18541 tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg agtatttgac
    18601 caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca aatcatcaca
    18661 gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg cagcgggaaa
    18721 acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac ggctattatg
    18781 ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg acgcttcgga
    18841 gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga tgaatggcgc
    18901 aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat ttttgccccg
    18961 ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta caaacaagat
    19021 gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata tcatcattgc
    19081 ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc tcaaaaaggc
    19141 gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct tccagctatt
    19201 gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt ttctgctaat
    19261 ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct actgctcaat
    19321 cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct tccatgtcct
    19381 aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg ccattattgc
    19441 ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa aatccgctac
    19501 tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc gcaagctagg
    19561 attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca gattttacaa
    19621 aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc taaaggtttg
    19681 gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct gaatttaccg
    19741 gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg acgagcaggg
    19801 cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca ttacgccata
    19861 gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca tgtacggcat
    19921 cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca tcctgaagaa
    19981 caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt gttaagccct
    20041 caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa ccgatattat
    20101 tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt ggatgaaatt
    20161 ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga caacgaacca
    20221 ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc caccctaatg
    20281 ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca atcgctttat
    20341 tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct gctcctcaag
    20401 taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa tttgaactga
    20461 ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt tgtctcagta
    20521 ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt tattatgatc
    20581 gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc ttccagcatg
    20641 aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag atcccggaag
    20701 aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt tatgggcaca
    20761 cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga gatccaggca
    20821 gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc gacaccggta
    20881 aaagaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat ctccggttcg
    20941 ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc ggcatttggc
    21001 caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa tgtccatgct
    21061 tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat gaatggggag
    21121 aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg cggaatctat
    21181 gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt tgaaaagctt
    21241 agccttttag gaagaaagct attgctagaa acattgccca atattttgga tggacaaaaa
    21301 ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg ggaacaagaa
    21361 gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg aatgcgtcct
    21421 tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc ggttgaagta
    21481 ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa aaaaaatcta
    21541 tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc agctggtaaa
    21601 ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca gattggtcag
    21661 caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga tatatggcat
    21721 tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg ttgaatgaga
    21781 tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa ttgatctatg
    21841 gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc gctaaagcaa
    21901 aaaaagtgga tgactgggtc aagacgcttc tttatctttc tgtataccaa ttagaatttt
    21961 tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca aaagtaaaag
    22021 gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag cgtcaaggag
    22081 caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa atcagcctgc
    22141 ctctatggat gactaaacga ctggtggatc aaatcggata cgaagaaact gaaaagctcg
    22201 gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc cgactttcca
    22261 gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa agccaagtca
    22321 gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg ttccacgatg
    22381 gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg caaatcgagc
    22441 ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg catattgctt
    22501 cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac gaacacaaag
    22561 tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc tacgctcaaa
    22621 agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat cggattttag
    22681 tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt cgttataaaa
    22741 aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta gaaagctgcg
    22801 caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc ttaaaagaag
    22861 aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag aggattgatg
    22921 ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact ttgtatccgc
    22981 atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa tgaggtgaaa
    23041 aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat caagactatg
    23101 ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac gggatgggtg
    23161 gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc gaagcttggg
    23221 aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa tcgattcaag
    23281 aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac ggtatgggaa
    23341 caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat gttggggata
    23401 gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat cattctttag
    23461 tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac catccaagga
    23521 aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat gtttctactc
    23581 atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg aacatgctgt
    23641 ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat aaagtaactg
    23701 agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt cttcttatcg
    23761 aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc gttatcaaat
    23821 cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg atttaatctt
    23881 ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc agactgcgat
    23941 tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa acattgttag
    24001 tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat acgtcaaagg
    24061 aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa cagcagtaaa
    24121 tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa tcatccaccg
    24181 agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa tcacggattt
    24241 cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga tgctaggatc
    24301 tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc agtcagatat
    24361 ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt ttgatggtga
    24421 atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta taaaagcact
    24481 tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg ctaaagaacc
    24541 tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag tattatcacc
    24601 agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa caaaagttat
    24661 cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc ctctgccaaa
    24721 agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg atcctaaaaa
    24781 gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt taggaatcgg
    24841 aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg atgtttctga
    24901 tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag tagcaacaaa
    24961 aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga cagacccggc
    25021 agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta gcggcacgaa
    25081 aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag caagtttgaa
    25141 agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt cagaggtaga
    25201 aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc ctaaaacaga
    25261 cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta gtcatgtagg
    25321 agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata tcaataatat
    25381 tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca gtcaggatcc
    25441 agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa gccaaggaac
    25501 agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa gttatttagc
    25561 tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca cggtggataa
    25621 agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag gaatggttgt
    25681 gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa aagactcttc
    25741 agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa aagaagaatc
    25801 ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag tgctgaataa
    25861 aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt gtttctatct
    25921 cttttgatca attttcaagc catatacttt gagaagtttg catggaataa cacctacttt
    25981 cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg acaaatcaga
    26041 aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca aacaagagga
    26101 agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt gatctttgag
    26161 agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa tcaattagtt
    26221 cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt agagcctgca
    26281 ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc gatcgaaccg
    26341 attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga agagatcaag
    26401 caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg tgatacgaca
    26461 gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc tggagcagga
    26521 aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg agagatttct
    26581 gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc tatctatggc
    26641 gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat tgaagcagga
    26701 gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa atttagagag
    26761 tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac aggtggtatc
    26821 gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa aagaaaacca
    26881 atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa gatagcacca
    26941 tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt ggaaaaagga
    27001 ggcgcagatt acatccacgt agatgtaatg gatggccaat ttgtcccaaa tatcacatta
    27061 gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga cgtccatctg
    27121 atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc agatatcatt
    27181 actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac aaaaagctta
    27241 ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat caaacatgtt
    27301 ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg cggacaaagc
    27361 tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag agagaacaat
    27421 tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa aatctgtaaa
    27481 gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga aaatccagtc
    27541 ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt agctgctgga
    27601 ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta tgtcgggata
    27661 gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc agtgggtgat
    27721 tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa agagtatacc
    27781 cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa aacatttgaa
    27841 cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt ggatcatctt
    27901 ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca tcagcttatt
    27961 atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat agtaagacag
    28021 ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa tcttacatta
    28081 aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc ttatgcaagt
    28141 aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat tgcagttatt
    28201 caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct aaaaatgaca
    28261 gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg tttcacggca
    28321 tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg tgaagaagtt
    28381 caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg tgacgatact
    28441 aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc aatccatcgt
    28501 taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt gggtacgaaa
    28561 tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc ggctaaaaga
    28621 aaaaatcgat agctatgatg acgtttcaca gcaaaacaag aattacgaga gagaaatcga
    28681 agcgttgaaa aaagaacttg agttgaatca gacgctgact agttacgaaa aagtaacggc
    28741 aaatgtcatt actcgttcgc ctgacagttg gcaggacatg ctgatcgtag acaagggttc
    28801 taaagacggt atcgaagcaa atatggcagt catgtcgcaa aaaggattga ttggccgagt
    28861 gatcgaggtc aatacggctt cgtctaaaat cgaattactg tcatcctcta atgaaagctc
    28921 caatcatttt ccagtacggg tatcttcggc taatggcgaa gcgtttggtt tgcttaaaaa
    28981 ctatgatgaa aagctccatg ccttagtggt gacccaatta actggtgata cggatatcaa
    29041 agaaggggat gttgtccaga catccggtct tggagggaat tctccagcta acttgccgat
    29101 cggtacggtt attaaaacga aaccagatag ttatgggctg gatcgggaag tttatgtgaa
    29161 accttatgca gaaatgtatg acgtgtcagt tgtgacgatt gtccaacggc tagcggagga
    29221 ttagtccatg ttgaagaaag aaacaatgaa gtactatctg ccaatcgttt tgttcttttt
    29281 gatgttgata gatggtcatt taacaagaat gctaggggag tggtcgaaag gcacctatat
    29341 gtcaaatgcc cactttctga tattggcatt attatgttgc agtatggcgt ttgaaaaacg
    29401 ttatttactg attaccacga ttgttctcgg ggctatctat gacgcttact atattggcgt
    29461 tatcggtatc tatgcagtag ctctcccttt aattgtatgg ttgatgtatg taatgaaaga
    29521 cgttatccat gtcaacatct ttactgaatt tttcagtatg atcatctttg tcacgggtta
    29581 tgaattgttt acgatggtgg tccagttgat ttttaaatta gcagtagtaa ataacacgta
    29641 ttttattaca aggtttttag gacctacact gctgttgaac atgattatat ttgtattatt
    29701 catttttccc tttaagaaat t
//
LOCUS       DAFBYI010000022        28845 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFBYI010000022 DAFBYI010000000
VERSION     DAFBYI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.022"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(138..662)
                     /locus_tag="LLD04_000840"
     CDS             complement(138..662)
                     /locus_tag="LLD04_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647789.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(655..1128)
                     /gene="tsaE"
                     /locus_tag="LLD04_000841"
     CDS             complement(655..1128)
                     /gene="tsaE"
                     /locus_tag="LLD04_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK5647790.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1251..2231)
                     /gene="pta"
                     /locus_tag="LLD04_000842"
     CDS             complement(1251..2231)
                     /gene="pta"
                     /locus_tag="LLD04_000842"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK5647791.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2384..3064)
                     /locus_tag="LLD04_000843"
     CDS             complement(2384..3064)
                     /locus_tag="LLD04_000843"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5647792.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3190..4059
                     /locus_tag="LLD04_000844"
     CDS             3190..4059
                     /locus_tag="LLD04_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5647793.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4085..4600
                     /locus_tag="LLD04_000845"
     CDS             4085..4600
                     /locus_tag="LLD04_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647794.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4647..5240)
                     /locus_tag="LLD04_000846"
     CDS             complement(4647..5240)
                     /locus_tag="LLD04_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647795.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5265..5444)
                     /locus_tag="LLD04_000847"
     CDS             complement(5265..5444)
                     /locus_tag="LLD04_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647796.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5521..6030)
                     /locus_tag="LLD04_000848"
     CDS             complement(5521..6030)
                     /locus_tag="LLD04_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647797.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6126..6506)
                     /locus_tag="LLD04_000849"
     CDS             complement(6126..6506)
                     /locus_tag="LLD04_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5647798.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6836..7585
                     /locus_tag="LLD04_000850"
     CDS             6836..7585
                     /locus_tag="LLD04_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK5647799.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7769..9046)
                     /locus_tag="LLD04_000851"
     CDS             complement(7769..9046)
                     /locus_tag="LLD04_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647800.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9125..9769)
                     /gene="pcp"
                     /locus_tag="LLD04_000852"
     CDS             complement(9125..9769)
                     /gene="pcp"
                     /locus_tag="LLD04_000852"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK5647801.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9968..10912)
                     /locus_tag="LLD04_000853"
     CDS             complement(9968..10912)
                     /locus_tag="LLD04_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK5647802.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10914..11600)
                     /locus_tag="LLD04_000854"
     CDS             complement(10914..11600)
                     /locus_tag="LLD04_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK5647803.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12074..12394
                     /locus_tag="LLD04_000855"
     CDS             12074..12394
                     /locus_tag="LLD04_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK5647804.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12548..13453
                     /locus_tag="LLD04_000856"
     CDS             12548..13453
                     /locus_tag="LLD04_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647805.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13450..14223
                     /locus_tag="LLD04_000857"
     CDS             13450..14223
                     /locus_tag="LLD04_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5647806.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14404..15828)
                     /locus_tag="LLD04_000858"
     CDS             complement(14404..15828)
                     /locus_tag="LLD04_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647807.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16039..16485
                     /gene="nrdI"
                     /locus_tag="LLD04_000859"
     CDS             16039..16485
                     /gene="nrdI"
                     /locus_tag="LLD04_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5647808.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16550..18532)
                     /locus_tag="LLD04_000860"
     CDS             complement(16550..18532)
                     /locus_tag="LLD04_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5647809.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18546..19442)
                     /gene="murQ"
                     /locus_tag="LLD04_000861"
     CDS             complement(18546..19442)
                     /gene="murQ"
                     /locus_tag="LLD04_000861"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5647810.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19445..20506)
                     /locus_tag="LLD04_000862"
     CDS             complement(19445..20506)
                     /locus_tag="LLD04_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5647811.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20717..21283)
                     /locus_tag="LLD04_000863"
     CDS             complement(20717..21283)
                     /locus_tag="LLD04_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5647812.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21311..22813)
                     /gene="eat(A)"
                     /locus_tag="LLD04_000864"
     CDS             complement(21311..22813)
                     /gene="eat(A)"
                     /locus_tag="LLD04_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5647813.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23071..24192)
                     /locus_tag="LLD04_000865"
     CDS             complement(23071..24192)
                     /locus_tag="LLD04_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5647814.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24318..25259
                     /locus_tag="LLD04_000866"
     CDS             24318..25259
                     /locus_tag="LLD04_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5647815.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25409..26305)
                     /locus_tag="LLD04_000867"
     CDS             complement(25409..26305)
                     /locus_tag="LLD04_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK5647816.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26574..27914
                     /locus_tag="LLD04_000868"
     CDS             26574..27914
                     /locus_tag="LLD04_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647817.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28132..28575
                     /locus_tag="LLD04_000869"
     CDS             28132..28575
                     /locus_tag="LLD04_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK5647818.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         8312 a         5984 c         5181 g         9368 t
ORIGIN      
        1 ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga ctgcgacacg
       61 acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga cagtttctca
      121 ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc taaaaattca
      181 cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc catttttcgg
      241 taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg aaaaagtaca
      301 cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag tcccattccc
      361 caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg aaattgactg
      421 tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag cagcaataaa
      481 ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg tggtaacagg
      541 agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc catcatcaaa
      601 agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac ttcttcactc
      661 atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt gctccccgta
      721 tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta aaatcagttc
      781 caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa cggaaagccc
      841 atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta cccgataaat
      901 atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg tgtatgtcgg
      961 acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca acgttgtttt
     1021 acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg aatgaccaat
     1081 tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt ttcatcttct
     1141 cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa agaaaaaatg
     1201 aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg ttaatcaatc
     1261 aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt tgctccacga
     1321 gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc ttcaaagtta
     1381 ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg aaatacaaag
     1441 actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac tgaaggaaca
     1501 taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag ttttttagcg
     1561 attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc ttttgtcgag
     1621 aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt tgcagaatct
     1681 acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca atccgaaaag
     1741 acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt ccgactgatg
     1801 ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt cgaatggatc
     1861 gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc aaagtaattc
     1921 acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct acgttcaaca
     1981 aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat cgtaaagcta
     2041 gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt ccctactaaa
     2101 atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat acgagcatca
     2161 gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct taaactatca
     2221 aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca ctattccaga
     2281 aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat taagtttttc
     2341 acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact gtttcaggca
     2401 gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt ttcgagaagg
     2461 gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga gatgttatga
     2521 tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt ccccacaaga
     2581 taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct gttaatcttt
     2641 cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga acggtgttga
     2701 gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca ggtgcgattc
     2761 caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc tttacgccag
     2821 gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga tcttgtccta
     2881 aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag atatgataca
     2941 tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt agatgcagat
     3001 aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga atgatttctt
     3061 tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc acaagggaca
     3121 atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca gaaaggaacg
     3181 ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt agatgcccat
     3241 atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc cggtgttgag
     3301 ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga tgaggtagga
     3361 atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga aggaaattca
     3421 ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat tctagatgct
     3481 aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa tcaagcgaaa
     3541 cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac gtataaaatg
     3601 gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt ggacagtatt
     3661 cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt tcatacagaa
     3721 gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga tctagccgtt
     3781 acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa aggaattgct
     3841 gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac gattggcgat
     3901 aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat gggaaatgca
     3961 gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga atctggcgta
     4021 ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga aaagagggat
     4081 atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc ggacaattgt
     4141 caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa caaagggcga
     4201 tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc agataagcct
     4261 gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa ccatgcagaa
     4321 aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga cagtccaagg
     4381 tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca tgaaaatgga
     4441 taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg atgaagatgc
     4501 tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta aagacaaaag
     4561 tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac ctgaaatgcg
     4621 gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc ataaagatga
     4681 ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa ttttttagcc
     4741 tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt cttcgccaat
     4801 ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc ttctgataca
     4861 ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa caaacgtgta
     4921 gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat gattgattct
     4981 tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc ttctacgata
     5041 cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa aatctctaaa
     5101 gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag aatcactaag
     5161 atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat agtttcctct
     5221 ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt taaaaaatct
     5281 tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc agttcggcaa
     5341 taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt gacttcttcg
     5401 tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc tcaacttcct
     5461 ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa tatccttctt
     5521 ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga ctttataata
     5581 aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt tcccgtagta
     5641 gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt tttctatatc
     5701 aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc cagtggatgg
     5761 attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg cattcgtagg
     5821 attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt cttttgtttc
     5881 gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt cactaatatc
     5941 atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta ttacgatgag
     6001 ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt ttttttaaag
     6061 aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg acaattttgt
     6121 tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa cgtcattttt
     6181 tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga ctgcaattct
     6241 gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc acaagcttct
     6301 ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc agcacttcct
     6361 agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct gattacttca
     6421 aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac aatgatggct
     6481 atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta agctcggatc
     6541 tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc tgtttttcct
     6601 ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac tcttcctgtt
     6661 cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca gaaaattatc
     6721 ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc atacaataga
     6781 ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct catacatgaa
     6841 gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa attattatct
     6901 tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca tcacttggct
     6961 gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat ccatcaatgc
     7021 ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta ttatcgttgg
     7081 gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga tcacattgga
     7141 tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa tccgaaaatt
     7201 cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc ttgtttcagg
     7261 attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca ttgctttttc
     7321 tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat attactggtg
     7381 gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc atggatctgc
     7441 aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc ttgcatttac
     7501 cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg aagaagaata
     7561 ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta aagaacactt
     7621 ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct tcggaaataa
     7681 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
     7741 taaacgcttc tgtcccagcc tctatttttt atagtaattc cttcagatac tttcttgcct
     7801 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
     7861 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
     7921 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
     7981 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
     8041 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
     8101 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
     8161 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
     8221 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
     8281 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
     8341 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
     8401 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
     8461 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
     8521 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
     8581 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
     8641 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
     8701 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
     8761 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
     8821 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
     8881 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
     8941 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
     9001 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
     9061 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
     9121 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
     9181 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
     9241 gacgatcgat cacttgatcg ggtgagaatg acacgtggat gaacccacct cgaattgccg
     9301 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
     9361 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
     9421 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
     9481 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
     9541 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
     9601 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
     9661 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
     9721 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
     9781 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
     9841 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
     9901 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
     9961 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    10021 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    10081 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    10141 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    10201 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    10261 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    10321 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    10381 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    10441 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    10501 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    10561 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    10621 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    10681 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    10741 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    10801 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    10861 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    10921 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    10981 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    11041 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    11101 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    11161 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    11221 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    11281 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    11341 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    11401 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    11461 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    11521 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    11581 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
    11641 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
    11701 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
    11761 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
    11821 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
    11881 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
    11941 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
    12001 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
    12061 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    12121 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    12181 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
    12241 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
    12301 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
    12361 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
    12421 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
    12481 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    12541 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    12601 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
    12661 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
    12721 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
    12781 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
    12841 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
    12901 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
    12961 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    13021 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    13081 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    13141 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
    13201 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
    13261 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
    13321 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
    13381 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
    13441 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
    13501 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
    13561 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
    13621 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
    13681 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
    13741 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
    13801 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
    13861 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
    13921 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
    13981 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
    14041 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
    14101 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
    14161 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
    14221 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
    14281 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
    14341 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
    14401 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
    14461 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
    14521 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
    14581 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
    14641 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
    14701 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
    14761 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
    14821 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
    14881 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
    14941 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
    15001 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
    15061 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
    15121 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
    15181 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
    15241 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
    15301 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
    15361 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
    15421 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
    15481 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
    15541 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
    15601 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
    15661 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
    15721 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
    15781 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
    15841 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
    15901 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
    15961 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
    16021 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
    16081 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
    16141 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
    16201 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
    16261 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
    16321 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
    16381 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
    16441 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
    16501 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
    16561 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
    16621 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
    16681 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
    16741 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
    16801 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
    16861 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
    16921 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
    16981 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
    17041 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
    17101 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
    17161 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    17221 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    17281 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    17341 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    17401 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    17461 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    17521 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    17581 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    17641 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    17701 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    17761 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    17821 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    17881 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    17941 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    18001 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    18061 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    18121 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    18181 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    18241 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    18301 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    18361 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    18421 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    18481 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    18541 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    18601 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    18661 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    18721 gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact
    18781 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    18841 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    18901 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    18961 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    19021 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    19081 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    19141 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    19201 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    19261 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    19321 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    19381 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    19441 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    19501 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    19561 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    19621 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    19681 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    19741 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    19801 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    19861 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    19921 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    19981 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    20041 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    20101 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    20161 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    20221 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    20281 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    20341 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    20401 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    20461 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    20521 tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata
    20581 cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt
    20641 gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa
    20701 taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa
    20761 agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat
    20821 ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga
    20881 tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac
    20941 gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt
    21001 ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg
    21061 tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta
    21121 ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg
    21181 cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa
    21241 gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag
    21301 attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg
    21361 tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt
    21421 gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg
    21481 gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga
    21541 tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt
    21601 ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat
    21661 tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc
    21721 cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg
    21781 aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt
    21841 cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat
    21901 tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct
    21961 gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac
    22021 ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg
    22081 ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc
    22141 tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt
    22201 tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg
    22261 ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc
    22321 cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat
    22381 tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga
    22441 cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg
    22501 tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta
    22561 acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt
    22621 gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg
    22681 tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat
    22741 ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt
    22801 cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    22861 taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc
    22921 aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt
    22981 tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt
    23041 acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct
    23101 aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc
    23161 taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc
    23221 tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag
    23281 cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc
    23341 tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa
    23401 atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc
    23461 tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc
    23521 aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct
    23581 tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat
    23641 aatcaatttt actttagaaa caagaacgtt ttttcgatca aaatgaagtt ttttagtaga
    23701 taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc
    23761 tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg
    23821 aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg
    23881 atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt
    23941 gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc
    24001 catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt
    24061 ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt
    24121 tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc
    24181 caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt
    24241 tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa
    24301 taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa
    24361 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc
    24421 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    24481 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    24541 tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta
    24601 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    24661 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    24721 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    24781 cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc
    24841 cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    24901 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg
    24961 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    25021 tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca
    25081 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    25141 tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat
    25201 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    25261 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa
    25321 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag
    25381 tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt
    25441 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    25501 cataaaattc caatagctct gataggattg gcttaaatgg tgtatgcaga aattccggta
    25561 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    25621 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc
    25681 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    25741 tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa
    25801 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    25861 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    25921 aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    25981 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    26041 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    26101 atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct
    26161 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    26221 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    26281 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    26341 tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt
    26401 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    26461 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    26521 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    26581 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    26641 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    26701 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
    26761 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    26821 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    26881 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    26941 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    27001 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    27061 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    27121 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    27181 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    27241 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
    27301 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    27361 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
    27421 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
    27481 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    27541 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    27601 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    27661 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    27721 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    27781 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    27841 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    27901 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
    27961 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    28021 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    28081 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    28141 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    28201 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    28261 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    28321 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    28381 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    28441 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    28501 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    28561 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
    28621 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
    28681 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    28741 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
    28801 atcagcgttc tgacctgagt attagagaga caaatggaaa aatag
//
LOCUS       DAFBYI010000023        28606 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFBYI010000023 DAFBYI010000000
VERSION     DAFBYI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.023"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(5..586)
                     /locus_tag="LLD04_000870"
     CDS             complement(5..586)
                     /locus_tag="LLD04_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5647819.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="LLD04_000871"
     CDS             complement(769..1395)
                     /locus_tag="LLD04_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5647820.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="LLD04_000872"
     CDS             complement(1417..2748)
                     /locus_tag="LLD04_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK5647821.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="LLD04_000873"
     CDS             complement(2903..3304)
                     /locus_tag="LLD04_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5647822.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="LLD04_000874"
     CDS             complement(3390..3596)
                     /locus_tag="LLD04_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647823.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="LLD04_000875"
     CDS             complement(3691..5193)
                     /locus_tag="LLD04_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK5647824.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="LLD04_000876"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="LLD04_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5647825.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="LLD04_000877"
     CDS             complement(5764..6381)
                     /locus_tag="LLD04_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK5647826.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="LLD04_000878"
     CDS             6602..7975
                     /locus_tag="LLD04_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5647827.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="LLD04_000879"
     CDS             8050..9162
                     /locus_tag="LLD04_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK5647828.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="LLD04_000880"
     CDS             complement(9234..10871)
                     /locus_tag="LLD04_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK5647829.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="LLD04_000881"
     CDS             complement(11039..11647)
                     /locus_tag="LLD04_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5647830.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="LLD04_000882"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="LLD04_000882"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK5647831.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="LLD04_000883"
     CDS             complement(13201..13953)
                     /locus_tag="LLD04_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647832.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="LLD04_000884"
     CDS             complement(14094..15044)
                     /locus_tag="LLD04_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5647833.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="LLD04_000885"
     CDS             complement(15045..15884)
                     /locus_tag="LLD04_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5647834.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="LLD04_000886"
     CDS             complement(15881..16627)
                     /locus_tag="LLD04_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5647835.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="LLD04_000887"
     CDS             16865..18235
                     /locus_tag="LLD04_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647836.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="LLD04_000888"
     CDS             complement(18290..19186)
                     /locus_tag="LLD04_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5647837.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19300..19470)
                     /locus_tag="LLD04_000889"
     CDS             complement(19300..19470)
                     /locus_tag="LLD04_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647838.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19467..20144)
                     /locus_tag="LLD04_000890"
     CDS             complement(19467..20144)
                     /locus_tag="LLD04_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5647839.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="LLD04_000891"
     CDS             complement(20235..21140)
                     /locus_tag="LLD04_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5647840.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="LLD04_000892"
     CDS             21315..22769
                     /locus_tag="LLD04_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5647841.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="LLD04_000893"
     CDS             22785..23816
                     /locus_tag="LLD04_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5647842.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="LLD04_000894"
     CDS             23823..25010
                     /locus_tag="LLD04_000894"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK5647843.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="LLD04_000895"
     CDS             25166..26548
                     /locus_tag="LLD04_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5647844.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="LLD04_000896"
     CDS             26520..26906
                     /locus_tag="LLD04_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647845.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="LLD04_000897"
     CDS             complement(27106..27774)
                     /locus_tag="LLD04_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK5647846.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27963..>28606)
                     /locus_tag="LLD04_000898"
     CDS             complement(27963..>28606)
                     /locus_tag="LLD04_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HBK5647847.1"
                     /translation="IKKLGIKVTTYTRKSRRYNSYRGQVGAVAKNRIHRRFYTSICHQ
                     KITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAVMEGLEE
                     AIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGL
                     LKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         7959 a         5844 c         5255 g         9548 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgtcc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28021 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28081 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28141 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28201 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28261 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28321 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    28381 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    28441 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    28501 cgatggatcc gatttttggc tactgctccg acttgtcctc gataagaatt gtagcgccgt
    28561 gatttacgtg tgtatgtcgt tacttttatt cctagcttct tgatca
//
LOCUS       DAFBYI010000024        28366 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFBYI010000024 DAFBYI010000000
VERSION     DAFBYI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.024"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            161..391
                     /locus_tag="LLD04_000899"
     CDS             161..391
                     /locus_tag="LLD04_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK5647848.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="LLD04_000900"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="LLD04_000900"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5647849.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="LLD04_000901"
     CDS             1802..1978
                     /locus_tag="LLD04_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5647850.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="LLD04_000902"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="LLD04_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK5647851.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="LLD04_000903"
     CDS             2593..3060
                     /locus_tag="LLD04_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5647852.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="LLD04_000904"
     CDS             3145..4029
                     /locus_tag="LLD04_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5647853.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="LLD04_000905"
     CDS             4143..4379
                     /locus_tag="LLD04_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK5647854.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="LLD04_000906"
     tRNA            4467..4547
                     /locus_tag="LLD04_000906"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="LLD04_000907"
     tRNA            4554..4625
                     /locus_tag="LLD04_000907"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="LLD04_000908"
     CDS             4750..4920
                     /locus_tag="LLD04_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647855.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="LLD04_000909"
     CDS             5140..5847
                     /locus_tag="LLD04_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5647856.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="LLD04_000910"
     CDS             complement(5907..6551)
                     /locus_tag="LLD04_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK5647857.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="LLD04_000911"
     CDS             6678..6965
                     /locus_tag="LLD04_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647858.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="LLD04_000912"
     CDS             7073..7393
                     /locus_tag="LLD04_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647859.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="LLD04_000913"
     CDS             7474..8787
                     /locus_tag="LLD04_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5647860.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="LLD04_000914"
     CDS             8784..9503
                     /locus_tag="LLD04_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK5647861.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="LLD04_000915"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="LLD04_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK5647862.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="LLD04_000916"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="LLD04_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK5647863.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="LLD04_000917"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="LLD04_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK5647864.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="LLD04_000918"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="LLD04_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK5647865.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="LLD04_000919"
     CDS             14940..15833
                     /locus_tag="LLD04_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647866.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="LLD04_000920"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="LLD04_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5647867.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="LLD04_000921"
     CDS             17770..18627
                     /locus_tag="LLD04_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK5647868.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="LLD04_000922"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="LLD04_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK5647869.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="LLD04_000923"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="LLD04_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK5647870.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="LLD04_000924"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="LLD04_000924"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5647871.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="LLD04_000925"
     CDS             21383..22141
                     /locus_tag="LLD04_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5647872.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="LLD04_000926"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="LLD04_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK5647873.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="LLD04_000927"
     CDS             23069..24100
                     /locus_tag="LLD04_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5647874.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="LLD04_000928"
     CDS             24097..24864
                     /locus_tag="LLD04_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5647875.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="LLD04_000929"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="LLD04_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK5647876.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="LLD04_000930"
     CDS             26829..27149
                     /locus_tag="LLD04_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK5647877.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="LLD04_000931"
     CDS             27149..27499
                     /locus_tag="LLD04_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK5647878.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..>28366
                     /gene="hprK"
                     /locus_tag="LLD04_000932"
     CDS             27666..>28366
                     /gene="hprK"
                     /locus_tag="LLD04_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HBK5647879.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYL"
BASE COUNT         9342 a         4797 c         6150 g         8077 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 gggggaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gtttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatct
//
LOCUS       DAFBYI010000025        28315 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFBYI010000025 DAFBYI010000000
VERSION     DAFBYI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.025"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(29..1513)
                     /gene="guaB"
                     /locus_tag="LLD04_000933"
     CDS             complement(29..1513)
                     /gene="guaB"
                     /locus_tag="LLD04_000933"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK5647880.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2018..2713)
                     /locus_tag="LLD04_000934"
     CDS             complement(2018..2713)
                     /locus_tag="LLD04_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK5647881.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(2803..3903)
                     /gene="ychF"
                     /locus_tag="LLD04_000935"
     CDS             complement(2803..3903)
                     /gene="ychF"
                     /locus_tag="LLD04_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK5647882.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(3912..4103)
                     /locus_tag="LLD04_000936"
     CDS             complement(3912..4103)
                     /locus_tag="LLD04_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK5647883.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4124..5014)
                     /locus_tag="LLD04_000937"
     CDS             complement(4124..5014)
                     /locus_tag="LLD04_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK5647884.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5001..5768)
                     /locus_tag="LLD04_000938"
     CDS             complement(5001..5768)
                     /locus_tag="LLD04_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5647885.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(5780..6493)
                     /gene="rsmG"
                     /locus_tag="LLD04_000939"
     CDS             complement(5780..6493)
                     /gene="rsmG"
                     /locus_tag="LLD04_000939"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK5647886.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(6725..8626)
                     /gene="mnmG"
                     /locus_tag="LLD04_000940"
     CDS             complement(6725..8626)
                     /gene="mnmG"
                     /locus_tag="LLD04_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK5647887.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(8643..10040)
                     /gene="mnmE"
                     /locus_tag="LLD04_000941"
     CDS             complement(8643..10040)
                     /gene="mnmE"
                     /locus_tag="LLD04_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK5647888.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10339..10722
                     /locus_tag="LLD04_000942"
     CDS             10339..10722
                     /locus_tag="LLD04_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647889.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(10890..11648)
                     /locus_tag="LLD04_000943"
     CDS             complement(10890..11648)
                     /locus_tag="LLD04_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK5647890.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(11666..12484)
                     /locus_tag="LLD04_000944"
     CDS             complement(11666..12484)
                     /locus_tag="LLD04_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5647891.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12481..12837)
                     /gene="rnpA"
                     /locus_tag="LLD04_000945"
     CDS             complement(12481..12837)
                     /gene="rnpA"
                     /locus_tag="LLD04_000945"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK5647892.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(12955..13089)
                     /gene="rpmH"
                     /locus_tag="LLD04_000946"
     CDS             complement(12955..13089)
                     /gene="rpmH"
                     /locus_tag="LLD04_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK5647893.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            13612..14946
                     /gene="dnaA"
                     /locus_tag="LLD04_000947"
     CDS             13612..14946
                     /gene="dnaA"
                     /locus_tag="LLD04_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK5647894.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15144..16274
                     /gene="dnaN"
                     /locus_tag="LLD04_000948"
     CDS             15144..16274
                     /gene="dnaN"
                     /locus_tag="LLD04_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK5647895.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16503..16745
                     /gene="yaaA"
                     /locus_tag="LLD04_000949"
     CDS             16503..16745
                     /gene="yaaA"
                     /locus_tag="LLD04_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK5647896.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            16732..17856
                     /gene="recF"
                     /locus_tag="LLD04_000950"
     CDS             16732..17856
                     /gene="recF"
                     /locus_tag="LLD04_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK5647897.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            17853..19799
                     /gene="gyrB"
                     /locus_tag="LLD04_000951"
     CDS             17853..19799
                     /gene="gyrB"
                     /locus_tag="LLD04_000951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK5647898.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            19819..22290
                     /gene="gyrA"
                     /locus_tag="LLD04_000952"
     CDS             19819..22290
                     /gene="gyrA"
                     /locus_tag="LLD04_000952"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK5647899.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22526..22825
                     /locus_tag="LLD04_000953"
     CDS             22526..22825
                     /locus_tag="LLD04_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK5647900.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            22875..23387
                     /gene="ssb"
                     /locus_tag="LLD04_000954"
     CDS             22875..23387
                     /gene="ssb"
                     /locus_tag="LLD04_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5647901.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23413..23649
                     /locus_tag="LLD04_000955"
     CDS             23413..23649
                     /locus_tag="LLD04_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK5647902.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            23792..25765
                     /locus_tag="LLD04_000956"
     CDS             23792..25765
                     /locus_tag="LLD04_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK5647903.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            25771..26223
                     /gene="rplI"
                     /locus_tag="LLD04_000957"
     CDS             25771..26223
                     /gene="rplI"
                     /locus_tag="LLD04_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK5647904.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26497..27864
                     /gene="dnaB"
                     /locus_tag="LLD04_000958"
     CDS             26497..27864
                     /gene="dnaB"
                     /locus_tag="LLD04_000958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK5647905.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8510 a         5640 c         5566 g         8599 t
ORIGIN      
        1 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
       61 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
      121 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
      181 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
      241 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
      301 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
      361 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
      421 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
      481 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
      541 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
      601 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
      661 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
      721 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
      781 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
      841 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
      901 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
      961 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     1021 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     1081 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     1141 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     1201 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     1261 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     1321 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     1381 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     1441 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     1501 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     1561 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     1621 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     1681 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     1741 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     1801 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     1861 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     1921 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     1981 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     2041 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     2101 taatgataac tactactgga tctaatactg gattgataac tggtgtcaat agtgttgtcg
     2161 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     2221 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     2281 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     2341 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     2401 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     2461 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     2521 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     2581 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     2641 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     2701 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     2761 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     2821 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     2881 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     2941 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     3001 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     3061 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     3121 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     3181 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     3241 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     3301 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     3361 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     3421 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     3481 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     3541 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     3601 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     3661 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     3721 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     3781 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     3841 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     3901 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     3961 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     4021 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     4081 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     4141 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     4201 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     4261 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     4321 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     4381 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     4441 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     4501 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     4561 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     4621 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     4681 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     4741 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     4801 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     4861 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     4921 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     4981 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     5041 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     5101 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     5161 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     5221 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     5281 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     5341 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     5401 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
     5461 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
     5521 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
     5581 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
     5641 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
     5701 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
     5761 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
     5821 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
     5881 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
     5941 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
     6001 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
     6061 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
     6121 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
     6181 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
     6241 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
     6301 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
     6361 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
     6421 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
     6481 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
     6541 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
     6601 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
     6661 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
     6721 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
     6781 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
     6841 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
     6901 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
     6961 ttttgatgta gccttcatat ttgatttgga cttctacttg ttcgatttct tttcgtgtca
     7021 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
     7081 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
     7141 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
     7201 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
     7261 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
     7321 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
     7381 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
     7441 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
     7501 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
     7561 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
     7621 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
     7681 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
     7741 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
     7801 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
     7861 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
     7921 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
     7981 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
     8041 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
     8101 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
     8161 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
     8221 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
     8281 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
     8341 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
     8401 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
     8461 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
     8521 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
     8581 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
     8641 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
     8701 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
     8761 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
     8821 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
     8881 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
     8941 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
     9001 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
     9061 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
     9121 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
     9181 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
     9241 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
     9301 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
     9361 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
     9421 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
     9481 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
     9541 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
     9601 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
     9661 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
     9721 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
     9781 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
     9841 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
     9901 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
     9961 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    10021 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    10081 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    10141 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    10201 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    10261 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    10321 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    10381 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    10441 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    10501 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    10561 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    10621 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    10681 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    10741 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    10801 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    10861 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    10921 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    10981 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    11041 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    11101 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    11161 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    11221 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    11281 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    11341 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    11401 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    11461 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    11521 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    11581 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    11641 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    11701 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    11761 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    11821 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    11881 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    11941 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    12001 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    12061 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    12121 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    12181 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    12241 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    12301 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    12361 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    12421 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    12481 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    12541 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    12601 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    12661 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    12721 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    12781 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    12841 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    12901 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    12961 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    13021 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    13081 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    13141 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    13201 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    13261 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    13321 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    13381 tttatccaca gacgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    13441 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    13501 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    13561 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    13621 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    13681 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    13741 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    13801 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    13861 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    13921 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    13981 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    14041 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    14101 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    14161 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    14221 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    14281 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    14341 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    14401 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    14461 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    14521 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    14581 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    14641 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    14701 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    14761 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    14821 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    14881 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    14941 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    15001 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    15061 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    15121 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    15181 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    15241 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    15301 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    15361 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    15421 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    15481 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    15541 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    15601 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    15661 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    15721 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    15781 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    15841 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    15901 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    15961 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    16021 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    16081 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    16141 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    16201 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    16261 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    16321 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    16381 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    16441 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    16501 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    16561 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    16621 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    16681 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    16741 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    16801 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    16861 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    16921 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    16981 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    17041 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    17101 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    17161 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    17221 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    17281 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    17341 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    17401 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    17461 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    17521 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    17581 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    17641 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    17701 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    17761 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    17821 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    17881 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    17941 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    18001 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    18061 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    18121 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    18181 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    18241 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    18301 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    18361 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    18421 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    18481 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    18541 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    18601 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    18661 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    18721 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    18781 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    18841 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    18901 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    18961 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    19021 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    19081 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    19141 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    19201 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    19261 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    19321 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    19381 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    19441 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    19501 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    19561 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    19621 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    19681 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    19741 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    19801 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    19861 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    19921 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    19981 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    20041 atgggtaaat accatcctca tggagaccgt gcgatctatg agtcaatggt acggatggca
    20101 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    20161 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    20221 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    20281 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    20341 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    20401 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    20461 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    20521 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    20581 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    20641 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    20701 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    20761 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    20821 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    20881 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    20941 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    21001 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    21061 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    21121 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    21181 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    21241 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    21301 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    21361 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    21421 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    21481 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    21541 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    21601 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    21661 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    21721 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    21781 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    21841 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    21901 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    21961 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    22021 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    22081 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    22141 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    22201 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    22261 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    22321 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    22381 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    22441 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    22501 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    22561 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    22621 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    22681 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    22741 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    22801 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    22861 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    22921 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    22981 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    23041 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    23101 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    23161 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    23221 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    23281 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    23341 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    23401 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    23461 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    23521 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    23581 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    23641 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    23701 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    23761 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    23821 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    23881 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    23941 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    24001 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    24061 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    24121 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    24181 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    24241 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    24301 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    24361 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    24421 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    24481 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    24541 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    24601 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    24661 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    24721 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    24781 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    24841 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    24901 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    24961 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    25021 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    25081 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    25141 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    25201 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    25261 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    25321 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    25381 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    25441 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    25501 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    25561 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    25621 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    25681 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    25741 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    25801 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    25861 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    25921 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    25981 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    26041 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    26101 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    26161 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    26221 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    26281 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    26341 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    26401 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    26461 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    26521 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    26581 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    26641 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    26701 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    26761 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    26821 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    26881 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    26941 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    27001 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    27061 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    27121 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    27181 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    27241 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    27301 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    27361 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    27421 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    27481 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    27541 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    27601 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    27661 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    27721 gatgatggaa acgagccaaa aaatgacaac atcatcgaag tcatcatcga gaagaaccga
    27781 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    27841 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    27901 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    27961 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    28021 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    28081 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    28141 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    28201 ttgaaattgc tccttgtgag tcagagttaa acggcaggct cacaacctct ttcttaaacg
    28261 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgaga
//
LOCUS       DAFBYI010000026        26756 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFBYI010000026 DAFBYI010000000
VERSION     DAFBYI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.026"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..707
                     /locus_tag="LLD04_000959"
     CDS             <1..707
                     /locus_tag="LLD04_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK5647906.1"
                     /translation="VNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQ
                     RLVVCDLGTDRVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDST
                     VRVLSYNKETGAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFA
                     VFEDGSHIQNKQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELL
                     KKDVYVPECVCVYFDR"
     gene            726..887
                     /locus_tag="LLD04_000960"
     CDS             726..887
                     /locus_tag="LLD04_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647907.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(1146..1580)
                     /locus_tag="LLD04_000961"
     CDS             complement(1146..1580)
                     /locus_tag="LLD04_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647908.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(1690..3549)
                     /locus_tag="LLD04_000962"
     CDS             complement(1690..3549)
                     /locus_tag="LLD04_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5647909.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(3667..4827)
                     /locus_tag="LLD04_000963"
     CDS             complement(3667..4827)
                     /locus_tag="LLD04_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBK5647910.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(5134..6558)
                     /gene="araA"
                     /locus_tag="LLD04_000964"
     CDS             complement(5134..6558)
                     /gene="araA"
                     /locus_tag="LLD04_000964"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK5647911.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(6574..7272)
                     /locus_tag="LLD04_000965"
     CDS             complement(6574..7272)
                     /locus_tag="LLD04_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5647912.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(7229..8890)
                     /locus_tag="LLD04_000966"
     CDS             complement(7229..8890)
                     /locus_tag="LLD04_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK5647913.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            9108..10508
                     /locus_tag="LLD04_000967"
     CDS             9108..10508
                     /locus_tag="LLD04_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK5647914.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(10760..11839)
                     /locus_tag="LLD04_000968"
     CDS             complement(10760..11839)
                     /locus_tag="LLD04_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5647915.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(11925..12884)
                     /locus_tag="LLD04_000969"
     CDS             complement(11925..12884)
                     /locus_tag="LLD04_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5647916.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(12896..14407)
                     /locus_tag="LLD04_000970"
     CDS             complement(12896..14407)
                     /locus_tag="LLD04_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5647917.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(14429..14992)
                     /locus_tag="LLD04_000971"
     CDS             complement(14429..14992)
                     /locus_tag="LLD04_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5647918.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(14998..15861)
                     /locus_tag="LLD04_000972"
     CDS             complement(14998..15861)
                     /locus_tag="LLD04_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5647919.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(15875..16771)
                     /locus_tag="LLD04_000973"
     CDS             complement(15875..16771)
                     /locus_tag="LLD04_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5647920.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(16946..18235)
                     /locus_tag="LLD04_000974"
     CDS             complement(16946..18235)
                     /locus_tag="LLD04_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5647921.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(18207..19724)
                     /locus_tag="LLD04_000975"
     CDS             complement(18207..19724)
                     /locus_tag="LLD04_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5647922.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            19922..20812
                     /locus_tag="LLD04_000976"
     CDS             19922..20812
                     /locus_tag="LLD04_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK5647923.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(20885..21709)
                     /gene="nadE"
                     /locus_tag="LLD04_000977"
     CDS             complement(20885..21709)
                     /gene="nadE"
                     /locus_tag="LLD04_000977"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK5647924.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(21721..23193)
                     /locus_tag="LLD04_000978"
     CDS             complement(21721..23193)
                     /locus_tag="LLD04_000978"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK5647925.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(23830..24192)
                     /locus_tag="LLD04_000979"
     CDS             complement(23830..24192)
                     /locus_tag="LLD04_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5647926.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            24415..25167
                     /locus_tag="LLD04_000980"
     CDS             24415..25167
                     /locus_tag="LLD04_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5647927.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(25307..26230)
                     /locus_tag="LLD04_000981"
     CDS             complement(25307..26230)
                     /locus_tag="LLD04_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5647928.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(26245..26517)
                     /locus_tag="LLD04_000982"
     CDS             complement(26245..26517)
                     /locus_tag="LLD04_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647929.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         7872 a         5578 c         4533 g         8773 t
ORIGIN      
        1 aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc
       61 atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc
      121 tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg
      181 atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag
      241 gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag
      301 acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga
      361 aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt
      421 caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg
      481 cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt
      541 ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca
      601 ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa
      661 aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga
      721 atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat attcctttct
      781 ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg
      841 atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata
      901 ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa
      961 ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt
     1021 aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct
     1081 atcataaagg taatattaca tttatattag ataactctag tttttattgt tatcaaagat
     1141 ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct
     1201 gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca
     1261 agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca
     1321 aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcttaagc
     1381 attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat
     1441 ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga
     1501 tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa
     1561 tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt
     1621 ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc
     1681 tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac
     1741 aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg
     1801 cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc
     1861 ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat
     1921 tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa
     1981 ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt
     2041 tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt
     2101 ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga
     2161 aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg
     2221 atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg
     2281 aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc
     2341 atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat
     2401 gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta
     2461 tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag
     2521 ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc
     2581 catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg
     2641 agaaagcata ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc
     2701 accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa
     2761 cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta
     2821 tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc
     2881 taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg
     2941 gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt
     3001 gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat
     3061 caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat
     3121 cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag
     3181 caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact
     3241 cattttcata taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc
     3301 taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca
     3361 ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga
     3421 tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact
     3481 gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata
     3541 tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt
     3601 agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct
     3661 ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt
     3721 ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa
     3781 tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc
     3841 attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa
     3901 tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt
     3961 accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc
     4021 ttcctgagtc gatcccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc
     4081 ttcttctgga aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac
     4141 agcatattta tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat
     4201 tttttctata ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac
     4261 tacgatgtaa tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa
     4321 ctgggcatcg cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc
     4381 tacttttgtg cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg
     4441 tcccacataa gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt
     4501 ttgtaccata cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac
     4561 aagctctttt actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc
     4621 agaagctttt tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa
     4681 atctggtgta agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg
     4741 caaatcatct attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat
     4801 agcatcaatc aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa
     4861 tttctgcaat tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga
     4921 gcagagtagg ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt
     4981 tcgagagcaa aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt
     5041 gataataaga ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt
     5101 cttaaaacta agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc
     5161 aactaatttt gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg
     5221 atgaccgcca cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt
     5281 ccataacaca cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac
     5341 ttcattgatc agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc
     5401 tcctgctttc ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg
     5461 agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga
     5521 ttccaagatt gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt
     5581 atctttccct cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc
     5641 ttccccagca aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg
     5701 tttcattcca tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa
     5761 gaaacggcga ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt
     5821 ttcttcataa tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc
     5881 gacttcttcg tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc
     5941 aacagtccaa ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt
     6001 tctcatgttg tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc
     6061 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
     6121 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
     6181 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
     6241 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
     6301 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
     6361 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
     6421 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
     6481 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
     6541 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
     6601 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
     6661 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
     6721 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
     6781 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
     6841 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
     6901 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
     6961 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
     7021 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
     7081 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
     7141 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
     7201 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
     7261 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
     7321 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
     7381 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
     7441 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
     7501 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
     7561 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
     7621 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
     7681 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
     7741 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
     7801 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
     7861 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
     7921 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
     7981 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
     8041 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
     8101 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
     8161 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
     8221 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
     8281 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
     8341 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
     8401 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
     8461 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
     8521 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
     8581 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
     8641 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
     8701 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
     8761 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
     8821 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
     8881 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
     8941 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
     9001 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
     9061 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
     9121 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
     9181 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
     9241 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
     9301 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
     9361 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
     9421 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
     9481 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
     9541 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
     9601 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
     9661 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
     9721 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
     9781 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
     9841 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
     9901 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
     9961 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    10021 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    10081 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    10141 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    10201 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    10261 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    10321 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    10381 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    10441 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    10501 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    10561 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    10621 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    10681 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    10741 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    10801 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    10861 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    10921 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    10981 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    11041 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    11101 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    11161 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    11221 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    11281 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    11341 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    11401 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    11461 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    11521 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    11581 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    11641 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    11701 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    11761 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    11821 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    11881 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    11941 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    12001 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    12061 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    12121 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    12181 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    12241 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    12301 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    12361 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    12421 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    12481 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    12541 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    12601 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    12661 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    12721 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    12781 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    12841 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    12901 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    12961 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    13021 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    13081 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    13141 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    13201 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    13261 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    13321 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    13381 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    13441 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    13501 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    13561 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    13621 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    13681 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    13741 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    13801 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    13861 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    13921 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    13981 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    14041 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    14101 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    14161 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    14221 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    14281 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    14341 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    14401 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    14461 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    14521 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    14581 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    14641 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    14701 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    14761 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    14821 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    14881 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    14941 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    15001 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    15061 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    15121 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    15181 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    15241 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    15301 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    15361 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    15421 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    15481 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    15541 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    15601 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    15661 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    15721 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    15781 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    15841 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    15901 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    15961 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    16021 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    16081 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    16141 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    16201 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    16261 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    16321 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    16381 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    16441 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    16501 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    16561 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    16621 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    16681 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    16741 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    16801 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    16861 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    16921 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    16981 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    17041 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    17101 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    17161 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    17221 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    17281 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    17341 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    17401 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    17461 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    17521 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    17581 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    17641 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    17701 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    17761 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    17821 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    17881 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    17941 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    18001 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    18061 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    18121 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    18181 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    18241 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    18301 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    18361 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    18421 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    18481 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    18541 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    18601 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    18661 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    18721 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    18781 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    18841 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    18901 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    18961 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    19021 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    19081 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    19141 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    19201 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    19261 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    19321 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    19381 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    19441 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    19501 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    19561 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    19621 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    19681 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    19741 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    19801 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    19861 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    19921 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    19981 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    20041 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    20101 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    20161 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    20221 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    20281 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    20341 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    20401 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    20461 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    20521 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    20581 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    20641 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    20701 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    20761 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    20821 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    20881 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    20941 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    21001 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    21061 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    21121 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    21181 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    21241 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    21301 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    21361 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    21421 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    21481 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    21541 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    21601 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    21661 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    21721 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    21781 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    21841 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    21901 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    21961 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    22021 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    22081 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    22141 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    22201 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    22261 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    22321 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    22381 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    22441 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    22501 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    22561 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    22621 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    22681 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    22741 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    22801 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    22861 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    22921 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    22981 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    23041 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    23101 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    23161 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    23221 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    23281 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    23341 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    23401 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    23461 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    23521 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    23581 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    23641 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    23701 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    23761 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    23821 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    23881 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    23941 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    24001 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    24061 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    24121 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    24181 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    24241 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    24301 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    24361 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    24421 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    24481 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    24541 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    24601 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    24661 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    24721 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    24781 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    24841 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    24901 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    24961 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    25021 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    25081 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    25141 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    25201 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    25261 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    25321 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    25381 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    25441 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    25501 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    25561 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    25621 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    25681 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    25741 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    25801 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    25861 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    25921 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    25981 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    26041 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    26101 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    26161 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    26221 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    26281 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    26341 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    26401 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    26461 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    26521 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    26581 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    26641 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    26701 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacaca
//
LOCUS       DAFBYI010000027        26543 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFBYI010000027 DAFBYI010000000
VERSION     DAFBYI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.027"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            176..2623
                     /locus_tag="LLD04_000983"
     CDS             176..2623
                     /locus_tag="LLD04_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5647930.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2853..3920
                     /gene="rlmN"
                     /locus_tag="LLD04_000984"
     CDS             2853..3920
                     /gene="rlmN"
                     /locus_tag="LLD04_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5647931.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4113..5216)
                     /locus_tag="LLD04_000985"
     CDS             complement(4113..5216)
                     /locus_tag="LLD04_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5647932.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5769..6317
                     /gene="aac(6')"
                     /locus_tag="LLD04_000986"
     CDS             5769..6317
                     /gene="aac(6')"
                     /locus_tag="LLD04_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5647933.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            6863..8524
                     /locus_tag="LLD04_000987"
     CDS             6863..8524
                     /locus_tag="LLD04_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5647934.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(8594..8941)
                     /locus_tag="LLD04_000988"
     CDS             complement(8594..8941)
                     /locus_tag="LLD04_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK5647935.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            9025..9966
                     /locus_tag="LLD04_000989"
     CDS             9025..9966
                     /locus_tag="LLD04_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647936.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9967..11010
                     /locus_tag="LLD04_000990"
     CDS             9967..11010
                     /locus_tag="LLD04_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647937.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            11016..12086
                     /locus_tag="LLD04_000991"
     CDS             11016..12086
                     /locus_tag="LLD04_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647938.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            12076..13029
                     /locus_tag="LLD04_000992"
     CDS             12076..13029
                     /locus_tag="LLD04_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5647939.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            13041..14051
                     /locus_tag="LLD04_000993"
     CDS             13041..14051
                     /locus_tag="LLD04_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5647940.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(14336..15157)
                     /locus_tag="LLD04_000994"
     CDS             complement(14336..15157)
                     /locus_tag="LLD04_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5647941.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(15254..15532)
                     /locus_tag="LLD04_000995"
     CDS             complement(15254..15532)
                     /locus_tag="LLD04_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647942.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            15800..17809
                     /gene="metG"
                     /locus_tag="LLD04_000996"
     CDS             15800..17809
                     /gene="metG"
                     /locus_tag="LLD04_000996"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK5647943.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(18288..18875)
                     /locus_tag="LLD04_000997"
     CDS             complement(18288..18875)
                     /locus_tag="LLD04_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647944.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            19050..20372
                     /locus_tag="LLD04_000998"
     CDS             19050..20372
                     /locus_tag="LLD04_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5647945.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            20527..21303
                     /locus_tag="LLD04_000999"
     CDS             20527..21303
                     /locus_tag="LLD04_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5647946.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            21296..21865
                     /gene="rnmV"
                     /locus_tag="LLD04_001000"
     CDS             21296..21865
                     /gene="rnmV"
                     /locus_tag="LLD04_001000"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK5647947.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            21858..22742
                     /gene="rsmA"
                     /locus_tag="LLD04_001001"
     CDS             21858..22742
                     /gene="rsmA"
                     /locus_tag="LLD04_001001"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK5647948.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            23193..23822
                     /locus_tag="LLD04_001002"
     CDS             23193..23822
                     /locus_tag="LLD04_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647949.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(23946..25055)
                     /gene="ugpC"
                     /locus_tag="LLD04_001003"
     CDS             complement(23946..25055)
                     /gene="ugpC"
                     /locus_tag="LLD04_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK5647950.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            25264..25686
                     /gene="mgsA"
                     /locus_tag="LLD04_001004"
     CDS             25264..25686
                     /gene="mgsA"
                     /locus_tag="LLD04_001004"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK5647951.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            26009..>26543
                     /locus_tag="LLD04_001005"
     CDS             26009..>26543
                     /locus_tag="LLD04_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5647952.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         8692 a         4711 c         5482 g         7658 t
ORIGIN      
        1 aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta
       61 agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact
      121 ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc
      181 acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca
      241 gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt
      301 cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc
      361 aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat
      421 taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta
      481 tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc
      541 aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga
      601 caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat
      661 tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt
      721 cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca
      781 ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag
      841 aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga
      901 aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc
      961 tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga
     1021 cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa
     1081 acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt
     1141 agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt
     1201 gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat
     1261 cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag
     1321 cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa
     1381 aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga
     1441 agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat
     1501 ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt
     1561 acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca
     1621 cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac
     1681 aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat
     1741 ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc
     1801 tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa
     1861 attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa
     1921 cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt
     1981 agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca
     2041 aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt
     2101 tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg
     2161 tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga
     2221 tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc
     2281 tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga
     2341 agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga
     2401 tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct
     2461 tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga
     2521 agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca
     2581 ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg
     2641 gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt
     2701 ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa
     2761 agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt
     2821 agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga
     2881 cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc
     2941 aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat
     3001 cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg
     3061 tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca
     3121 tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag
     3181 taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc
     3241 tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca
     3301 atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt
     3361 acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg
     3421 ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata
     3481 acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat
     3541 cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt
     3601 atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg
     3661 atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat
     3721 atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa
     3781 aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac
     3841 gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga
     3901 aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca
     3961 gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct
     4021 ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg
     4081 acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg
     4141 ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg
     4201 gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct
     4261 tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt
     4321 ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc
     4381 agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc
     4441 gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa
     4501 accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga
     4561 tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt
     4621 tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa
     4681 gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt
     4741 gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag
     4801 agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag
     4861 ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt
     4921 acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt
     4981 tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg
     5041 gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt
     5101 gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct
     5161 ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc
     5221 atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg
     5281 gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa
     5341 caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga
     5401 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
     5461 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
     5521 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
     5581 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
     5641 tttattttat aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat
     5701 ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg
     5761 gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt
     5821 ctgatttact gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag
     5881 aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag
     5941 gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg
     6001 tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag
     6061 aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa
     6121 caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga
     6181 accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct
     6241 taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc
     6301 gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag
     6361 aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa
     6421 gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta
     6481 agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga
     6541 aactggcttt tgaacaccgt ttattcagat aagggactgt gacaagactt ttgtcacagt
     6601 ttctttttgt ttattctttt ttatctttgt atggcaataa tagacaatat aacgaaaatt
     6661 atttaatggc ttttggaaga ccatggaaaa ccttgtacaa agctattttt tctgaaaaat
     6721 tccttgaaac ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt
     6781 ttttaataat tattcattaa actcttaaac aacttcagaa aatattaatt attctgaaaa
     6841 ttccaataaa ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg
     6901 cggccttgta cttgctggat gttatggcgg cagttcagcc acaagcagta gttcagcaaa
     6961 aagcggttca gcagatggag gaggcgtatt taacttagtc gtgcctcaag aaatgcctac
     7021 agctgactta tcagttgcaa cagatacaat cagttttaca gcattgaata acgtttatga
     7081 aggaatttac cgtcttgatg aagacagcaa accacagccg gcaggagcta gcgagttggc
     7141 agaagtcagt gaagacggat taacttacaa aatcaaatta cgagaagacg caaaatggtc
     7201 aaatggtgat cctgttacag cagcggatta cgtttatggc tggcaacgaa cagtagaccc
     7261 agctacagct tctgaatatg cttacctgta tgcgccagtt gaaaatgcag aagacattac
     7321 agctggaaag aaagataaat cagaactagg gatcaaagca gttggtgact atgaattaga
     7381 aatcaaattg acgaaacaaa caccatattt ccaatatctg cttgctttcc catcattttt
     7441 cccacaaaaa caatccgtag tggaagaaaa tggggatgcc tatgcatctg caagtgataa
     7501 agctgtttat aatggaccat tcacattaga agattttgat ggaccaggaa cagatacaga
     7561 atggacttat aagaaaaatg atcattactg ggacaaagat acagtaaaac tttctgaaat
     7621 caaagtcagt gtagtaaaag aatcttctac tgcattgaac ttattcaaag atggccaagc
     7681 agatgacgtg atcttgtctg gtgagttagc acagcaaaac gcaaacgatc cagcttatac
     7741 ttctgttaaa gaagcgcgga caagctatat cgaattcaat caacgtgaaa agaattcacc
     7801 attcaacaat gtgaacctaa gaaaagctat ttcttattct atcgatcgtg aagctttagt
     7861 aaaacaagta ctaggtgacg gatcagttgt ctctacagga ttgatcccat cagatatggt
     7921 aaaaagtcca acaacgaacg aagactttgc aaaagaagct ggaaaattag tttcttatga
     7981 caaagataaa gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt
     8041 agaatttgat ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca
     8101 aaactcaatc caagaaaact tggatggcgt aacagtcaaa ccaacacctg ttccattctc
     8161 tgtacgtctt gaccgctcaa cttcgggtga ttttgacgca gtactaggcg gatgggctgc
     8221 tgactatgca gacccaagca gctttactga tttgtttgtc acaggtaatt catacaaccg
     8281 tggtcgctgg agcaatgcag aatatgacga agcagttaaa gcagcttcat caacagacgc
     8341 tggcgatgaa caagcccgtt gggatgattt ccaaaaagca atcaagatca tctctgatga
     8401 tatgggtgtc gttccggtgt accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa
     8461 aggtatcgtt caccatgcgg caggcgcttc atgggattac aaatggacat acgttgaaga
     8521 ataatcgtta ataccgtgag catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg
     8581 ttctcatatt cacttatcct aacaaagaaa aaagcatgaa aatagcagaa agagcaatta
     8641 gtattccggc aatgatcagc caaaagctgt acataccgta gctggcagac gagagcgagg
     8701 aaaattctgg ttttttcttt cggttccttg ttcgtgcaag atggaccgat cgctgaaaat
     8761 gatcgaagaa acccgaagaa aagacccata aaaaaccgcc gataatcaga aacggcaatg
     8821 accataagaa tagatcatta gataattgaa tgagtgtaaa ggaatggttg atgagatttt
     8881 ttattaaaat aaccagaatc agtagaaaag ctactgcgta ggggatagat ttttttttca
     8941 tttgattgct cctttatttt tggttcatta tctaataatg tagcgaaaca tctgtcagaa
     9001 gtcaaaagct agggggaaac attagtgaat agttatatca aatatgtatt aaaacgtgtc
     9061 tttttcatga tcatcacgct ttggttaatt gcaacaatca cctttttctt gatgcaattg
     9121 ctgccgggca cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta
     9181 aataaacaag tcggtttgga taaaccagtt atcgtacaat atgggattta tctttctaat
     9241 ttgcttcaag gagattttgg aatttccttc caatttaaaa accagccagt cgcacatttg
     9301 ttagctggaa gaatcggacc atccttgcag ttagggttac aggcaattat ctttggaaca
     9361 gtcctaggga cgattttagg aacgatctct gccatgaagc aaaatacatg ggcagataca
     9421 tcttctacac tcgtagcgat attaggtcgt tccattccaa actttgtatt tgctgtttta
     9481 ttacaatata tctttgcaat caaattacaa gttttgccaa tcgcaaaatg ggatggcttt
     9541 gtgtacacaa tcttaccaac aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg
     9601 tttatccgta cggaaatggt ggaagtactg catagtgatt atgtggaatt agcaaaagca
     9661 aaaggattga gccgttggga aatcgccttt aaacatggtc ttcgaaatag tttgattcca
     9721 ttgatgactt tattaggacc gttagctgta gcattgatga ccggatcact ggtggtagaa
     9781 aatatctttg ctattcctgg tatcggggaa caattcgtta agtcaatcac aacaaatgat
     9841 tatcctacga tcatggctgt aacgatcctt tactcattta tgctgatttt tgttattttg
     9901 atcgtggatc tattgtacgg tctagttgat ccaagaattc gtgtttcaga agggagccga
     9961 ggataaatgg aaatgatacc aaagcgttat gaaacagttg cagatatccc tgcggatgaa
    10021 tttcttccat tacaaaaaaa taccgaagaa gaacgtgaac agattactgc accttctttg
    10081 aattttatcc aagattcatg gcgccgttta aagaaaaaca aagctgccgt tatttcaatg
    10141 gttttactat tggtcatcat tttgatctct gtgatcacaa tctttgtttc tcctcatgat
    10201 ccaactgccc aaaacgtggc ttatatcaat ttgcctcctc gtattccggg aatcaatatc
    10261 gatggattga atggaaaagc aatggttggt ggtgaacttg ttgataaata tgctcaagca
    10321 aacgtccctt caaacgtaaa tttcttttta ggaacagacg gtttgggacg tgatgtatta
    10381 agccgcttat ttatgggaat gagaatctcg ttgctgatcg catttattgc tgcattacta
    10441 gatgtaacga ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat
    10501 aacgtgatgc aacgtttttt ggaaatctta tcaggaattc caaacctcgt tgtcatgatt
    10561 ttgatgttgg tcgtttttga accaggaatt ttttcaatcg tggctgctat ggccatcaca
    10621 aactggatac caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa
    10681 tatgttttag ctggattgac attaggtgaa tcaagaatga aaattgcatt caaacatatt
    10741 ttgccgaata tctctagtgt cattattatt caaatgatgt ttagtattcc atctgctatt
    10801 ttcttcgaag cctttttaag ctttatcggt ttaggtctta cgcctccgtc agcttcatta
    10861 ggaacgatgt tgagcgaagg gtacaagaca ttcctgtatc tgccttatct attatggatt
    10921 ccggcagtaa ctttatcggt tatcatgatt ggctttaact tacttgctga tggattgcgt
    10981 gatgcctttg atcctaaaat gaaagagtga agacgatgac gaaaatttta gaagtgaaag
    11041 acttagaaat ctcttttgat acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt
    11101 ttcatttgaa taaaggagaa acattggcga tcgttgggga atctggaagt ggaaaatcag
    11161 tgacgaccag aagcatcatg cgcttgctgt caagcaacgc caatatagat gctggtcaaa
    11221 tcctctttaa aggtaacgat atcgttggaa aatcagaaag agagatgcaa aaaatccgtg
    11281 gtaaagaaat agcgatgatt ttccaagatc caatgacctc tttagatccg acgatgccca
    11341 ttgggaagca agtcgctgag tcccttagaa agcataataa agtttcaaaa aaagagggac
    11401 aaaaagctgc attagaattg cttaaattag ttggaatacc cgaagctgag aaacgtatca
    11461 acagttatcc gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac
    11521 tggtatgtta tccggaaatc ctgattgctg atgaacctac aacagcgctt gatgtgacta
    11581 ttcaagcaca gattttggag ctgctgaaag aaattcagac aaaaatcgat acatcaatta
    11641 tctttatcac tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt
    11701 atggaggaag aatcgttgaa gttggaacat cggaagaaat tttctacaac ccgcaacatc
    11761 cctatacttg gggactgctt ggttcaatgc cgacattaga tagtgcgaac gatcgattgt
    11821 atgctattcc tggttctcca ccggatttat tgaacccgcc aaaaggagac gcattctatc
    11881 caagaaatga attcgcgatg aaaatcgatg cagaacaaga accaccattt tttgaactat
    11941 caaaaacaca caaggcagca acttggctac ttgctccgca ggcacccaaa gtgacaccgc
    12001 cagaagaaat ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat acttacggtc
    12061 aaggaggaat tgctgatgcc aaataaacta cttgaagtta aaggattgaa acagtatttc
    12121 aatgtaggaa aaaagaatga agtacatgct gtaaatgaca tcagctttca catttatgaa
    12181 ggagaaacat tcggtttagt tggagaatct ggaagcggaa aatccactac tggccgtacg
    12241 atcattcgct tgaatgaacc tactgatggt gagatattat tcgacggtca agatataacg
    12301 aagatcaaag ataaaaaagg tttgacgaaa ttccggcatg atgttcagat gatctttcaa
    12361 gatccatacg cttcgttgaa tcctcgtatg aaagtcagag atatcattgc cgaaggaatt
    12421 gatgttaatg gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg
    12481 agaactgttg gattgaatcc aagccacggt actcgttatc ctcatgaatt ttcaggtggg
    12541 caacgccagc gtattggtat tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt
    12601 gatgaaccaa tttctgcact agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa
    12661 gatctccaaa aagaacatca attgacctat ctatttattg cacatgattt gtcgatggtt
    12721 aaacatatta gtgaccgaat cggtgtaatg cataacggct tgttattaga gatgggaaca
    12781 agtgatgaaa tctataatca cggtgttcat ccttatacag aaagtttgtt atctgctatt
    12841 cctctaccag atccagacca tgagagaaaa cgcagaagaa tcaaatatca acctgaacca
    12901 gatgacggac aagtacgtca acttcgtgaa atcgcaccag aacattttgt ctatgcaact
    12961 gagcaggaag tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt
    13021 gctaactagg agggacatag atgctaaaag cttttaaatt ccgtatgtat ccgacagaag
    13081 aacaaaaaca gcaattgatt cgtacttttg gatgtgctcg atttacctat aaccatttgc
    13141 tgaaaaagcg gcagaaaagt tggcaacaaa caggagtagc caatttttcg ctcacacccg
    13201 ctacattaaa aaaagaatac ccattcttaa aagaagtaga cagtcttgct ttagcaaatg
    13261 cacagcttaa tttggatcgg gcttttcgca attatttcaa gggacgtgcc agcttcccaa
    13321 agcttaaaac gaaaaaaagt atgtggcaat cttatacgac caacaaccaa acccgtacga
    13381 tctatctgga aaatggctac ttaaagttac caaaacaaaa agaactaatc aagataaatc
    13441 agcatcggcc agttgaagga tcgattcgct ccgctacgat ttctgctcga tataatgaag
    13501 aattttatgt cgcactactt tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat
    13561 ggatcgggat cgcctatcat ccgaagacat tgatccaaac atctcgacct ctagaagtaa
    13621 ctttaccaaa atttcaccag acagaagaaa agctgcaaca tgcacagcgg aaattgaatg
    13681 tgaaagtccg ctccgctcac catagaaaga tcaggttaga tcaggcaagc aattatcaga
    13741 aacaaaagag aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga gaagattatc
    13801 ttgaacaatt atctgggaaa ttggtcaaac aatatgacta cttatttgta gaatcttttc
    13861 caaaagaaga ggcacatgct gatttttcta ttcacgactg gcataaatta ataacaaaat
    13921 tgcgttataa gtcccaatgg tataataaaa aatttctttt gatcaatacc gacggagcag
    13981 aagaaagcaa ttccgtaaga aagagccaag tagtagaaaa aatgggcaga cattcggtta
    14041 tcaagggata aaaaatgaat gaaactgata ggagcgttag ggttagcctt gttaataaaa
    14101 agtcttaatg acttttgttt ctaggaattt ttcttttgaa gaaaagctca ctaattatga
    14161 caaaccaata aaacaatagc agaaaaagtt cgtacgagct ttttctgcta ttgttaattt
    14221 tttattcaga aagtttacga atacatcaaa attctgaaac aaaaaacgta actcacaaag
    14281 agttgttttt gcgttttttc ttgaatgatt aggtctgaat cttttgctca caaccttatt
    14341 catggtgccc cggcttgagc tgatagattt ttgaaggacg gccgattcct acaggacgtt
    14401 caccgatctc atcgaaatat tggagcatag ctttcttaaa attggaatga tcgatcgttt
    14461 tataatcgat ccctaagaat ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt
    14521 tacctaaaac ttgtagaact tgcggctcgt gttccatttt attgactaca cggttgaatg
    14581 ctttcaagat gatttgacta tggtcaaaag ccaaagtatc tttgccagaa gactctccag
    14641 tgactaaatc aagcgagata gccacttctc cgctagataa atggatcttg ttactatgac
    14701 gttctagtgt gaaccagcgt acttccttgg catcatctcc ggcgattagc ggttcttctc
    14761 caataaaggc taagtagctg actgtgacga cccagcctcg cggatctcga ttaggcgtac
    14821 tgaatgtatg cagttgctca atattttctt ttgaaataac cacaccagtt tcttcctttg
    14881 tttctcttaa gacgctttct ccagttgact catccttttg gacaaagcca ccaggcaatg
    14941 cccatgaatt tcgataagga tgcccttttc tttggatcaa caaaactttc agctgatctt
    15001 cttctttgtt atagcacatc agtacgatat cgacagtcaa agagggcttg tcatattctg
    15061 gacgttcctg tttttgatac cagttaagaa attcttcttc acttgcttta tgttcataat
    15121 ataatttttc ttcttcttta gaagcaaaac ggttcatctg accacctcca ttgacagtaa
    15181 aagtactttt taccttattg tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa
    15241 tctaggttat catttaaata tgaaaaataa atggaagtat gagattgaat aaaaagaaaa
    15301 gcagataaaa cggcaatact ttataccgct cttcacgaat ggcaaagaag ccacaaataa
    15361 cgatatatac gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat
    15421 gcccatggag gttaaaataa ccatacatac gaatataaaa atagctgaat attaaatgtg
    15481 ctaatgaaag aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc
    15541 cagtccctta ctaagataga tagtctttat tataccacgt gtgaggggag aaataaaaag
    15601 ccgtttttta gtagatatga aacaaaacaa aaacccgcat ttattcaacc tattcatcct
    15661 tttaaaggat agattgaacg tagatagaag tcgtttatcc agtctttttc attttgaaaa
    15721 aaagctaaca gcatgctttt tttatggtat actagcaagg aaacgaattc gcatcatgcg
    15781 ataaaggagg aagagccccg tggcagaaaa ggaaactttt tatatcacta cgccaatcta
    15841 ttatccaagc ggcaaacttc atatcggcaa ctcgtatact acgattgcat gtgacgcaat
    15901 tgctcgttat aaacggttga tgggctttga tgtgttttat ctcactggag tggatgaaca
    15961 tggtcaaaaa atcgaaacaa aagcagaaga actaggtgta aaaccgcaag aatatgtaga
    16021 taaaatggcg gctgatgtca agaaattatg gaaaacgctt gatatcagtt atgataaatt
    16081 cattcgcacg acagatgatt accataaagc agctgttcaa aaaatatttg atcgtttatt
    16141 agaacaggga gatatttact taggtgaata tgaaggctgg tactctgttt ctgacgaaga
    16201 attctttaca gaaacacaac ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg
    16261 tggtaaagcg ccaagtggac acgaagtaga attggtaaaa gaagaatcat acttcttccg
    16321 catgagcaaa tacgcagacc gtctattgga atactatgaa gaacatcccg aattcatcca
    16381 gccagaatct cgtaaaaacg aaatgatcaa caactttatc aaaccaggat tagaggatct
    16441 tgcagtttca cgaacaactt ttacttgggg agtaccagtc aagagtgatc cgaaacatgt
    16501 ggtgtatgta tggatcgatg cattatccaa ttatattact gcactaggat atggctccga
    16561 agatgaaagt ctattcgaaa aatactggcc tgcagatgtt cagatggtcg gtaaagaaat
    16621 cgttcgcttt catacgatct attggccaat catgctgatg gcccttgatt tgcctctacc
    16681 taaaaaagta tttgggcatg gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa
    16741 aggaaatgtc gtttatcctg aaatgcttgt ggaacgttat ggtttagatg cactccgtta
    16801 ttatttgctt cgtgctgttc cgtttgggtc agatggtgtc tttacgccag aagattttgt
    16861 ttcccgtgtc aattatgatc tggcaaatga tctagggaac ttattgaatc gaacgattgc
    16921 tatgatcaac aaatattgtg atggactcgt tcctgactat gcctcacttg tcactccatt
    16981 tgatagtgaa ctgtctacga cggctgctaa cgttgtcgga cgttaccatg atgcaatgga
    17041 aaaaatggaa ttcaatacag cattggctga aatctgggtg ctgatttctc gtgcaaacaa
    17101 atacattgat gaaacagaac catgggtatt agcaaaagac gaagaaaaga aagctgaatt
    17161 gaatagtgtc atgatccatt tagctgaatc tttgcgtatc gcagcgatat tacttcagcc
    17221 aatcatgaca gaaacgccag agaagatctt caatcaacta ggattggact ctgaaacaat
    17281 gaatcttgaa ggattacact ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg
    17341 tacaccaatc ttcccacgtt tagatatgga agaagaagtt gcttttattc aagaaaaaat
    17401 gtctgaaggt actcaaacaa acgaagatac agtcaaatgg gatccagaag aaacagaact
    17461 tgtttcaacg aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt
    17521 agcagaagtc atcaactgtc aaaaagtaga aggggcagat aagttgctgc aattccgact
    17581 agatgctgga gacagccaag accgtcaaat cttgtcagga atcgctgaat tttatcctga
    17641 tccaagcgaa ttgatcggga aaaaagtcgt cattgtagca aatctgaaac cacgaaagat
    17701 gcgtggacaa atcagtcaag gaatgatcct ttctgcagaa gcaccagatg gctctttaca
    17761 agtaatcgaa gcaccaaaat ccatgccaaa tggctcagaa atcgcataga aattcaaagg
    17821 gggtatgaca ctagttgtgt cataccccct ttttccgaaa tttgagaagc aattttcgta
    17881 aattccttct tattacttga agccaatcat ataatacaga aacagagaac tatccaaaat
    17941 aaatcctaga aaaagaagta cgagcttcgc tgatttttgt taatttagaa taatactaat
    18001 aggaatcgta aaaacagaaa aaaagcataa tgataaaggg tcgggcagat tttaattgat
    18061 tcatctggta ctctttttta ttgtccttac attcataaag tttgaatgca atgactttat
    18121 tacatctata tttatatcta acagtagcgt tgatgtgtta attataagtt ctatcacttt
    18181 atgttcaatt agtttgtgct cttatatgtg tgattgaaaa tatagataaa aaaacaatca
    18241 tcggctttga cttcgatgat tgttttcttt attttgtaaa aatcctatca cttgatttta
    18301 tggtaacctt ttatttttgt cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat
    18361 gggatcgaaa agacatacca gaaaatcgta cgtccgatac cataatgttg atagagcttt
    18421 gtcaacatca cgtgtaacaa gatcaaccct aaaagcaagg agccagctat aaaccaatga
    18481 ttatcccaat gcatgacttc actaagtgcc atcactccaa aaggatagaa ataaaaatag
    18541 atcccgacta aggtcatccg ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa
    18601 atcgctgaaa gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga atacaacatc
    18661 ccccacaaga tcatcacagg acctttgatc aatgcaacga ttgcgatgaa caaagtcagc
    18721 aatattagtt tttgtgataa cgttgcaaca gtcattaatc caaataaaat taatgtgaac
    18781 caatgccaat gggactgctg ttgttcttta cgggaaaaat cgacccggtt ggataaataa
    18841 tctgctaatt tcggataaga acgcttaaaa tccataaact gttcctcctt cgtggaaaag
    18901 gtaggtttag tatacgaaaa ataaaaaaga aatacatccg tcttaagagg gagtttttca
    18961 ctgaaagtgg agaaaaaaga atggtaaatt aaaatattct cagttatact gaaaattata
    19021 ccaaatttca aagaagaaag aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc
    19081 tgtttggcgc attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa
    19141 ctgctgctgt tactgctagt actcagtcta caagtttgac attgctagct tggcttttgg
    19201 gtggtttctt aacgatctgt gctggattga cagtggctga attagctaca gcaatcccgg
    19261 aaactggtgg agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc agctttttat
    19321 taggatgggc gcaaagcttg atttactttc ctgctaatat tgctgcatta tcgattatct
    19381 ttgcgacaca attgacgaac ttattacaat tatctaccga ttatcttttg ttgattgcag
    19441 tgatcaccgc tgtttctgtg acagggttga atttgttagg gacaaaagtc ggtgcttctg
    19501 tacaatcagt gacgttgatt gttaaattaa taccaattgc tgtgattgtc atatggggat
    19561 tattgacccc aggacaagga actgtccagt tatttccaat cgaagctgga aaagatgtta
    19621 cttttgtcga aggattgagc agtgcgttgt tagcaacgtt gtttgcttat gacggctggt
    19681 taggtgtcgg tgcaatggca ggggaaatga aacggccgga aaaagatttg cctaaagcaa
    19741 ttattcttgg tttgagtttt gtaacagtcg tttatctatt gatcaatttt gtttttttga
    19801 aaactttgcc gattgatcat cttgccggta atttgaatgc agcttctgaa gcatcagacg
    19861 tcatttttgg aggaatcggt ggaaagctgg tcactattgg gatcttgatc tcggtatacg
    19921 gtgcattgaa cggttataca ctaactggga tccgcgtgcc ttatgcgatg gctttagaag
    19981 acgagctacc tttcagtaaa cagctgacaa agctttcaaa gaagtttacg gttccttatg
    20041 ttcctgcggt tttccagtta gcagtagctt gtattatgat gagccttggt tctttcgact
    20101 ttttaacaga catgttgatt tttgtgatgt ggctattcag tttactgatt tttatcggtg
    20161 tctttatttt aagaaaaaaa gcgccggaat tgccacgtcc atataaagta ccactttatc
    20221 caatcgttcc gattattgcg attctcggtg ctattttcat tttaggtatg acgatgatga
    20281 cgcaaacgaa attagcattg atcggtatag gtgttacctt gataggtatc ccagtctatt
    20341 atcaaaaaaa gaaaaaacgc tcagaagagt aaaatatttc aacaacagac agaaaaagta
    20401 cgaactaggt cctctagatc ttcgtgcttt ttctatttca ataaaacaag ttcttctatt
    20461 ttgatttttg ctacttctgc aagtagtgct tatgtgctaa agtaagaatg aaaaggagag
    20521 ataaacatga tatttgattc tcatacccat ttaaatgctg aacagttcaa tgaagatatt
    20581 ccagaaacga ttcagcgagc acaagaaatg ggggtaacga aaatggcggt cgtgggcttc
    20641 gatacgccaa cgattgaaaa gtctttgcaa ttaagccatg actatccaaa tatatacagt
    20701 attatcggct ggcatccgac agaggctggc agttatacga aagaaatcga aaataaatta
    20761 caagaacaat tgactttacc aaaggttgta gcattagggg aaattggctt ggattatcat
    20821 tggatggaag acccgaaaga agtccaagat aaagtatttc gcagacagat tgctatcgca
    20881 aaagaaatga acctaccaat cagtattcat actagagaag cacttgaaga cacgtatcaa
    20941 atcctaaaag aagaagatgt tcgagatatc ggtggaatca tgcatagttt cagtggtgat
    21001 tatgaatggg caaaacgatt tcttgactta gggatgcata tttcatttag cggtgtcgtc
    21061 actttcaaaa aagcattaga cgtccaagag gctgcagcaa atgtaccaat cgaacgatta
    21121 cttgtggaaa cagatgcccc ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa
    21181 ccaggctata ctcgttatac agtagaaaag attgctgaac ttcggaatct tccgacagaa
    21241 gaagtggctg cccaaacatg gacaaatgcg caccgattat ttggtctaag cgaacatgga
    21301 taagatgaag atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg
    21361 ggaagtagtt gatgcagata cgatagaaac aatagggtct gcaatcaatg aagagatcct
    21421 gatgcaaatt gaacatgccc aagagactcg tggagtgatc gtttttaccg atcctgattt
    21481 ttcaggagaa aaaataagaa aaatcattat ggaagctgtt cctcaggcaa aacatgcttt
    21541 tttgccgcgc acacaagcta aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag
    21601 tgatgctgct attttagaag cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc
    21661 tgtttctgaa atcactagac aagacctttt agactatggt ttaatagctg gggcacaagc
    21721 aaaagaaaaa cgagaaaaac taggtgacga gttgcgcatc ggttacacga acggcaaaca
    21781 attggaaaaa cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa
    21841 aatagtggag gaacaacatg agtgaatatc gtgaaattgc gacaccctct agaacaaaag
    21901 agattttatt gaaacatggt ttttcattta aaaaaagttt aggacaaaat tttctgactg
    21961 aaccaaatat tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga
    22021 tcgaagttgg accaggaatc ggtgcattga cggaacaatt agccaagcat gcaaaacaag
    22081 tcgtagcatt tgagatcgat gatcgtttga tccctgtatt ggctgatacg atgcaaccct
    22141 atgacaatgt gactattgta catcaagata ttttaaagac cgatttgtca acagctgtta
    22201 gagaaacatt tcatgaggag cttccgttaa aagtcgtagc gaatttacct tactatatta
    22261 cgacaccgat catgatgcat tttcttgaat cagatttggt tgtggatgaa ctagtcgtca
    22321 tgatgcaaaa agaagtagct gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt
    22381 cattatcgat tgctgttcaa tattatatgg aagccagttt agcattcatc gtaccaaaaa
    22441 cagtatttgt ccctcagcca aatgtagatt cagcgatttt gaaactgaca agaagagata
    22501 taccggcagt cgaagtgaca gacgaaaaag catttttccg attgacaaaa gcggcatttc
    22561 agcaaagaag aaaaacgctt tggaataact tgcagcattc gtatggcaaa gatgaccaga
    22621 caaaagcatg gttagcaaaa agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg
    22681 agactttgtc attacaagaa ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt
    22741 aaaaaaagag gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    22801 gccaaacaaa aatagttcct catgtttttc gtttgtttgg gcttattacc gcagatgcaa
    22861 gcttttgaac accgtttaag aaagaggttg tgggcctgcc gtttaactct aagtcacaag
    22921 gaacaatttc aaaaaacagc ttctcatgtt tttgagttag ttggtcttat tacttgatgc
    22981 aagcttttga ccactattat gccgaacaag gagatgtgac acaaattttg tcacatctcc
    23041 tatttttgtt tcttgatcaa aaaattctta tacttgatac tcacactcat tttgtatgga
    23101 tttagaaaag aatatgtgtc ttgacagtta ttagtaagtg agttatacta agtcgacatt
    23161 tatgtgtcaa gacatattag gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa
    23221 cgatttctgc tttattgatt gggatggggg tcattatccc aatggtcatg cctaaaatca
    23281 tgattccacc agcttccttt actttggcgt ctcatgtgcc attatttatt gcgatgtttt
    23341 ttacaccagg agttgcagtg gctgttgcgc taggtacgac atttggtttc tttttaacaa
    23401 cgccggttat catcgcattg cgagcattgt cacatcttgt gtttgcgttg attggggctt
    23461 ggtacttgca aaagcatccg agtatcgttt taaaaaatgg acagtttact tttgctaatt
    23521 ggcgtttcca agggttcaat tttgtgatcg gtgtgattca ttctttagca gaaatgctag
    23581 tagtcagcat cttttacttt attggtaata tgcctgaaac atattattct caaggatatt
    23641 tttatacagt attcatcttg atggggattg gtgggcttat ccacagttta gttgattata
    23701 atattgctta ttttatagca ggaacactca gcaaatattt tgatatcccc gtatttacgg
    23761 cagcaaaaaa agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa acgacatatt
    23821 agacatgatc tctcaaaaaa aaaactctca tctagtaatg gataataaat cctctaagat
    23881 gagagttttt tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg
    23941 gcaatttatg gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg
    24001 atgttgaagg caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt
    24061 gagatgaact cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg
    24121 gaaacgacaa cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca
    24181 ctgtggatgt cttctggacg aataccgaag ataacttctt ttccttcata acctttttca
    24241 actaatactt tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata
    24301 acaccatttt tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca
    24361 acgaacatat tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat
    24421 ccgtctttca tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta
    24481 acatagatgg tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct
    24541 acacgtaatt tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc
    24601 cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt
    24661 tttcgtttca agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg
    24721 atgtctgctt tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc
    24781 atatgaggat acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc
    24841 acgtcattca tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc
    24901 gcgatcattc gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat
    24961 tcacggtctt tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat
    25021 tttttatata cgtttttcag agccatttct accataatac gttccacact ttctttttta
    25081 gttttattaa ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa
    25141 caagaatttt ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa
    25201 gaaataaaaa ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct
    25261 cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca
    25321 acagcgtata aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag
    25381 aggatcacgg aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg
    25441 gatcagcaaa ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga
    25501 gacccgctgg ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat
    25561 gtctatgaga ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg
    25621 gaagccggct tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca
    25681 ttttgatgga taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa
    25741 agctaaacaa actgataata tgcgatataa tacgaacgta ttggataata taaacaataa
    25801 tattagtttt tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga
    25861 tcccaaaaat aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc
    25921 ctcttggacg aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat
    25981 gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat
    26041 tgctattatg ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga
    26101 gaccccattt attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt
    26161 tggtcctttt tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt
    26221 ctcgaaatct gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta
    26281 cggattcttt ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag
    26341 tgtcacgctg atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt
    26401 gccattgttc agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc
    26461 gattcaatca attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag
    26521 aagtttagcg atccacgcta aat
//
LOCUS       DAFBYI010000028        26460 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFBYI010000028 DAFBYI010000000
VERSION     DAFBYI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.028"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(99..1229)
                     /locus_tag="LLD04_001006"
     CDS             complement(99..1229)
                     /locus_tag="LLD04_001006"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK5647953.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LLD04_001007"
     CDS             complement(1878..3080)
                     /locus_tag="LLD04_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5647954.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LLD04_001008"
     CDS             complement(3140..3343)
                     /locus_tag="LLD04_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK5647955.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LLD04_001009"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LLD04_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LLD04_001010"
     CDS             4880..5692
                     /locus_tag="LLD04_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5647956.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="LLD04_001011"
     CDS             complement(6716..6913)
                     /locus_tag="LLD04_001011"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5647957.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="LLD04_001012"
     CDS             complement(7059..7937)
                     /locus_tag="LLD04_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5647958.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="LLD04_001013"
     CDS             complement(7976..9094)
                     /locus_tag="LLD04_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK5647959.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="LLD04_001014"
     CDS             complement(9091..11190)
                     /locus_tag="LLD04_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK5647960.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="LLD04_001015"
     CDS             complement(11241..12062)
                     /locus_tag="LLD04_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5647961.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="LLD04_001016"
     CDS             complement(12059..12868)
                     /locus_tag="LLD04_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5647962.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="LLD04_001017"
     CDS             complement(12889..13383)
                     /locus_tag="LLD04_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5647963.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="LLD04_001018"
     CDS             complement(13395..13883)
                     /locus_tag="LLD04_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5647964.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="LLD04_001019"
     CDS             complement(13960..16656)
                     /locus_tag="LLD04_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5647965.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="LLD04_001020"
     CDS             complement(17084..18157)
                     /locus_tag="LLD04_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5647966.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="LLD04_001021"
     CDS             complement(18154..18981)
                     /locus_tag="LLD04_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647967.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="LLD04_001022"
     CDS             complement(18982..19788)
                     /locus_tag="LLD04_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5647968.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="LLD04_001023"
     CDS             complement(19800..20885)
                     /locus_tag="LLD04_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5647969.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="LLD04_001024"
     CDS             complement(20902..21444)
                     /locus_tag="LLD04_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5647970.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="LLD04_001025"
     CDS             21702..22691
                     /locus_tag="LLD04_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK5647971.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="LLD04_001026"
     CDS             22688..23425
                     /locus_tag="LLD04_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK5647972.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="LLD04_001027"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="LLD04_001027"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK5647973.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="LLD04_001028"
     CDS             23989..24621
                     /locus_tag="LLD04_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5647974.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="LLD04_001029"
     CDS             24706..25353
                     /locus_tag="LLD04_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5647975.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="LLD04_001030"
     CDS             25358..26341
                     /locus_tag="LLD04_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK5647976.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8102 a         5586 c         4485 g         8287 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgca
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacggt catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
//
LOCUS       DAFBYI010000029        26128 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFBYI010000029 DAFBYI010000000
VERSION     DAFBYI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.029"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            355..1173
                     /locus_tag="LLD04_001031"
     CDS             355..1173
                     /locus_tag="LLD04_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5647977.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1192..1599
                     /locus_tag="LLD04_001032"
     CDS             1192..1599
                     /locus_tag="LLD04_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647978.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1919..3004
                     /locus_tag="LLD04_001033"
     CDS             1919..3004
                     /locus_tag="LLD04_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5647979.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            3841..4173
                     /locus_tag="LLD04_001034"
     CDS             3841..4173
                     /locus_tag="LLD04_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK5647980.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4764..5093
                     /locus_tag="LLD04_001035"
     CDS             4764..5093
                     /locus_tag="LLD04_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647981.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            5080..5421
                     /locus_tag="LLD04_001036"
     CDS             5080..5421
                     /locus_tag="LLD04_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK5647982.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5535..5774
                     /locus_tag="LLD04_001037"
     CDS             5535..5774
                     /locus_tag="LLD04_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647983.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5841..6080
                     /locus_tag="LLD04_001038"
     CDS             5841..6080
                     /locus_tag="LLD04_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647984.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            6207..6896
                     /locus_tag="LLD04_001039"
     CDS             6207..6896
                     /locus_tag="LLD04_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBK5647985.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            6902..8068
                     /locus_tag="LLD04_001040"
     CDS             6902..8068
                     /locus_tag="LLD04_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK5647986.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            8150..8821
                     /locus_tag="LLD04_001041"
     CDS             8150..8821
                     /locus_tag="LLD04_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5647987.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8874..10850
                     /locus_tag="LLD04_001042"
     CDS             8874..10850
                     /locus_tag="LLD04_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5647988.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            10863..11615
                     /locus_tag="LLD04_001043"
     CDS             10863..11615
                     /locus_tag="LLD04_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5647989.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11631..12392
                     /locus_tag="LLD04_001044"
     CDS             11631..12392
                     /locus_tag="LLD04_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647990.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            12405..12665
                     /locus_tag="LLD04_001045"
     CDS             12405..12665
                     /locus_tag="LLD04_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647991.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12662..14752
                     /locus_tag="LLD04_001046"
     CDS             12662..14752
                     /locus_tag="LLD04_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5647992.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(14788..16845)
                     /locus_tag="LLD04_001047"
     CDS             complement(14788..16845)
                     /locus_tag="LLD04_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBK5647993.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(16990..17181)
                     /locus_tag="LLD04_001048"
     CDS             complement(16990..17181)
                     /locus_tag="LLD04_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647994.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            17369..17824
                     /locus_tag="LLD04_001049"
     CDS             17369..17824
                     /locus_tag="LLD04_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647995.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            17821..18063
                     /locus_tag="LLD04_001050"
     CDS             17821..18063
                     /locus_tag="LLD04_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBK5647996.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18081..18383
                     /locus_tag="LLD04_001051"
     CDS             18081..18383
                     /locus_tag="LLD04_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647997.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            18452..20506
                     /locus_tag="LLD04_001052"
     CDS             18452..20506
                     /locus_tag="LLD04_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5647998.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            20506..23118
                     /locus_tag="LLD04_001053"
     CDS             20506..23118
                     /locus_tag="LLD04_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBK5647999.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            23115..25493
                     /locus_tag="LLD04_001054"
     CDS             23115..25493
                     /locus_tag="LLD04_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648000.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            25554..26096
                     /locus_tag="LLD04_001055"
     CDS             25554..26096
                     /locus_tag="LLD04_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648001.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
BASE COUNT         9444 a         3978 c         5117 g         7589 t
ORIGIN      
        1 gtatatgatc taagtcttgc ttctagtatg aatatgaccg atgtatacga aaagttatta
       61 atactagaaa atgaatatga tcaattcaat tcaaatcagc agcgaaaagt aaagaaacag
      121 atagactaga atagctattt tcgaaaagca atatttaagg tataagttat acctttaata
      181 ataaaagtat aagttatacc ataaatatat atggtataac ttatactttt attattaaaa
      241 accgctatat ccaaacgttt ttagatatta ttatttaagt tatactttta atgttgacca
      301 tttccgatat ataagttata ctttaattat caattaaaga ttgggaggat ttacatggat
      361 acttttcaag ggactacatt tacagttggc aattttaaag gaggagtagg taaaactaag
      421 atagtcagca tgcttgctta tgacaatgca atgataagaa atagaaaaac gcttattatt
      481 gatttagacc ctcaagcgaa cttaacacaa gtcatagcaa gaactttcgg tataaccgat
      541 atcaaaacca ctataacaaa tggtgtcgca aatggagaat tagcaagtag tattattaag
      601 gtaaatgaaa atcttgacct tctggcatgt gacactagtt ttcgatcatt ctccgctttt
      661 gctaataaac atgaaaatga attagaccaa gttactgtct tgaaaaaatt acttcttcca
      721 ataaagaaca attatcaaga aatatttatt gatgtaccac caaccattag cgaattttcc
      781 gataatgcta tggcagcttc agactattcg ataatttcat ttcaaacagc tgaagaatcc
      841 ttagagggag tcaacaaata tgtaaattat caaaaattca tggtcgatag atacgatctt
      901 gatttgcaaa ttatagatat tgtaccttgt atggtagaac caaatgatga ttttgatgaa
      961 gaaatcctag aagaagcaat tgaaaaatac ggaaccgtgg tatccaaaaa tctcattcat
     1021 tatcaaaaaa gattgagaag gtattctaaa attggtatac atttaagaaa atacaaaaat
     1081 ggaaattttg atcaatggga ttataaagca caccaagtat ttataaatat tttggcagaa
     1141 ctagatgcta gacaagagtt tttaaataaa taataaggag ttgcataagt aatgcctaaa
     1201 gattttgata ttcctaaaat gatggagaat ggtgaatttg agccattatt acccaataac
     1261 aaacgccaag atgtgaaaat aagcggtgaa tataacttta gcaatagtgc attgagtaac
     1321 acaagaaata ttacattgaa aaaaagaggc cgaccagttt caattcctga taaaagaaac
     1381 aaggtatcag ttcctaaaaa gatctctccg gcattaaata gcaaactctc aattttacaa
     1441 gactatatga cggagttaca aagtgaaaaa gggagaattt ctttcgaaaa aattgttaat
     1501 acattagcag actcatatat ccagcataga ctaggagtag caaaagaaga acacataaga
     1561 gaagaaatac aagaagagtt tgaaaaatta ccaaaataat tttttttcat gcggtattca
     1621 attaaagtat aagttatact agataaatga atattaaagt ataagttata cattaaatat
     1681 ttaaggtata acttaataaa caacgtgtaa atttgcgatt aaatcaacat tataaaatta
     1741 ttatttaaag tataagttat acattatttg tatttaaagt ataacttata cttatttttt
     1801 taatatataa cttatacctt aaatactgaa agtgttttat tcacccttaa tacatggtat
     1861 agttcacttg ttgaaaatcc tgaatatata caagtttcta agaagaagga gaaatttaat
     1921 gtctacagaa aatcaatctc aaaaaatgat aattaaaaat tggggatata aagcaacgaa
     1981 tgtttatatt gaaaaatatt ataaatttcc tagtttttta attcagaatt ctaaatactc
     2041 taaaatgaaa gacagctcaa aaatagccta tatgcttttt aaaaatgaat ttcgtagagc
     2101 cttacatgaa aaatggatcg acgatgatgg tctattatat ttggaattta cacaagcaga
     2161 cctaatggac aaattaaatt gctaccaagg caaagtaaaa accattttaa acgagttaga
     2221 aacatataat ttgatttcag tcgtaaaagg agaatttaac ccaaaaacga aaaaaaatga
     2281 aaaaaataaa tactatttac ttcaacctga aataagtgcc gatgatcttt ttattcaaaa
     2341 ttctttcgat gacgatttaa gcgaaactat tgataaatca acctcttctc ggaatgcgga
     2401 aatcgcacgt cgagaggaaa ctgctaaaac ccttgataac actggaaatg cggaaatcgc
     2461 acgtcgagaa aaaacagctg aaacccttga taacactgga aatgcggaaa tcgcacaaga
     2521 ttattatatt aataaatctt tagacactaa aagacactta aaagacactg aaacagacca
     2581 attacaaaat caagttttgc tagataactt cgttgaaatc atgaaagaag acagcataaa
     2641 cacatttgta ccagaaaagg tattaagctt gataaaaaca ttttctaaca cttacgaaga
     2701 agcccaacaa accgtcacca caatacacaa agcaaaacat aaagccgagg aacttgagaa
     2761 gacggtagtt gtgtatgaag aacttgaatc ttacggaatt gatgcggata aaggattata
     2821 tatgacgttg ctgaaagctt atcaaaaaca aaagactgag aaagtaaata atttgcagaa
     2881 tttgattttt gtgtatgtaa aaaactggtt tattgaaaaa gctattgcgg caaagcttgc
     2941 aaacgaacaa agagaaacat ttaacgaatt accaacagtt agtacggaaa attggctaga
     3001 gtagttgata aaaagcgaac actataaaaa tgatcaattt taaattagaa cttttttaca
     3061 attttaattt tattacaaat gttacaaata aatagttatt attccggagt ttttagatta
     3121 aaagtttatg aaaacaatca caaacgtagg ttttaatgtc ataatcgtta gatattttag
     3181 ttatttttca ttcaaaaaaa cagggaacta gtgtttgcat atgtattttt ttgagttata
     3241 ataattcatg aagagaggta attcacttat ttacccaagc tgaaaagcct gatattactt
     3301 cttttcaggc agtgctgcta atattcaatt acactttttt gtataaattt agttgtttta
     3361 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata
     3421 aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctacca
     3481 accttcgtcg acgtccacag gtggcattaa gtctataccc cttaatgcgt ttacctttca
     3541 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     3601 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     3661 atgttataaa aatgtccgtg gggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     3721 cgcatgtttg aatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     3781 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     3841 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     3901 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     3961 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     4021 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     4081 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     4141 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     4201 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     4261 aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt
     4321 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     4381 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     4441 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     4501 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     4561 tcgtgtgctg cagtacatgt agatacttgc gaggacgttg atcattggat caatcgagca
     4621 tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     4681 tatatttttg agtgaacgaa aaacgataaa gatattcgtt atgaagtaaa actaatttag
     4741 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     4801 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     4861 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     4921 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     4981 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     5041 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     5101 atacgattgg gcacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     5161 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     5221 gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat
     5281 gatgaaggta atgaatggtt taaaatgacg cttacggatg ataatggaga cgaattatct
     5341 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     5401 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     5461 gagtcacgac gaataataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     5521 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     5581 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc
     5641 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     5701 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     5761 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     5821 caagaaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     5881 aagaaatgga agaaaagttg ctaaaaatga tgaaacaaaa acaccttaaa agattatctg
     5941 tcatgcaata tatcaatgat atgaagataa caggaaaaga gaaagcttgt ttgttaggtt
     6001 caatgaaaaa ctttgaacag ttgaggcgaa cttatgtaaa aacaggttcc aattgccaac
     6061 tattattaga agtttcttga cgattatgat atgaaaatga tcaattatga tgtggcattg
     6121 ttattgaata aaaagaatag taaaatttaa catggtttat cttggttaga tgtaggtaaa
     6181 ttaattcata tatagaaggt agatagatga ataaaaacaa taaaacttta tttaatcctt
     6241 acgtgggcgt aatcattgtt tttcttcttt ttagttattt tgcttatcaa ggttatcaaa
     6301 aggacaaaat cgctgaaaaa gtagatggtg taacaaaaac aatccatatt caggaacagg
     6361 ctaaaaccta tcaagataat ttaaccagtt taacggatat ttctaagaga caactgatta
     6421 ctaatcaaaa acaggcatta gaaaaggatc tcaaaggtga aattgctgga aaacttgttt
     6481 tttcttctac aggatttatt ctccctgttt ttcaaggagc gaatagcgac acgcttagtt
     6541 taggagtagc ttctacttat tatctagata ctgaaatggg aaatggaaat tatattcttg
     6601 ctggttataa cgtagataca ccaagcgttt tattatccga tatgactgat ttatctgaag
     6661 gagaagaaat tgatctaatc gatgctcatt acaagtatca gtatgaagtc tccaaaagag
     6721 gaaaaatttc tgattacagc caggtagttc aatctaagga aaacaattcc tttttatcct
     6781 tgccaaaagc aggtaaaaaa ccactactca cactgttagc ctttcaagca aataatatga
     6841 aaaagtgcga tgtgatccag tgtgaattag tcaatgtaga aaaaatcaat aaataaggga
     6901 agtgagtcac attcaagaac ttgtagaaaa attgatcaat aaaatgagtg ccatttttct
     6961 gttactgttg gtatccattc tatatttaat atggacggtg gggttaaatc ctcctacgat
     7021 attatttatt tatgataatg ctgcacagtt agtgacgttt ttcgtaggct gtttgtttct
     7081 tttgaatctg tacaaggtaa agagaaacga tctattcttc attgcagcag gcattctttt
     7141 ttaccttttc ttttcttttt ttagaagcta ccgaggagat actttctatg gtgatctgat
     7201 cgttccgaca attattgcta ttattttgat tgtaaaatgg acgaaattta ctgtaattga
     7261 tcgtagcttg tatcttgtag tattctatgt ctttttactc gtaacggttt atcgagtatt
     7321 tactgagata ccagtaccag aaggacaaag tatttggaat ccatcgaaca aattatcaga
     7381 tatttggatc aatacaaata cgataggtag ttctttaatg acattagccc ttcttatcac
     7441 aggatttgct agttcatttg gaaaatggta catgcgattg ctatcattac cagcgttgat
     7501 tcttgctggg gcaacgattt gggtttgcca atcgaaagca gcattatatg cgcttgttat
     7561 cttcattatc ttagaaattt tacctaagaa ggtttttcga aatacacact tgtccttttg
     7621 gatgtatgca gcagtttcgg ttctagcttt accgatttct tattttgctg caatgtcaga
     7681 caaagttcac ttatttacag gacgagaaga aatttggcgc aaattctatg aaacattagg
     7741 cgaaaaaaca ggacaaatat ttgtagggat gaaacctttc tttttccata gaggaaatca
     7801 gattctaggt aatcataata cgtatcacgc actcgtcaat cactatggat tgattggttt
     7861 tgttctatcg attattcttt tgcttagttt cttatggttg ctcgtaagaa aacgtgagct
     7921 gacaaatgga caactaacat tcttgtgggc ttttttagcg atcatgtgtc aatcgttcat
     7981 ggaagaaaca ttaacgacaa tcagttgggt gccaattgta ttcttaatgt tagcaatggc
     8041 tacacaccaa tataaaacca ccagataaat gaaatagaat agtaataaga gaaaacactt
     8101 tttcgttgtg aaaaagtgtt ttttgttttg tctgaaagga ggcagatgga tgaaaaaatg
     8161 gttatttggt tttttaggag tcgctttaat cgtcgtttgc agtgtgtttg gctatgttag
     8221 ctaccaaaaa catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga
     8281 tcaaatcaat gcacacgcaa agagttataa ggaggatgca acgaacgtca ataatgatat
     8341 gtctttgggg caaatgttgt ccatacaaaa agaagcaatc gaaatgggcg tcaataaaca
     8401 agtatttgca caaattcaga ttcctgcact tggtttagca ttgccaattt ttaaaggggc
     8461 aaatcaatac actttgagtc ttggagcggc tacttatttt tatgaagatg ccgaaatggg
     8521 taaaggaaac tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttctga
     8581 tattcaaaag ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg
     8641 ttacaaggta acgcgtaaat ttatcgtacc tgagtacttt aaattaatcg atggcgttcc
     8701 tgaagagaac tctttcttgt ctttaccaaa gaaaggagag aaacccttat taacgttgtt
     8761 tacttgtgtt tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata
     8821 acaagaattt aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa
     8881 taaatcggta tgtaaaaaaa gctggaatga ttgttggaat gttaggaatc attgcaggct
     8941 gctttcaggg gtatcgagct tatgcagaag atgtcacgca aaaaacgcca ccagaaaggg
     9001 tcaatatcac ggttcataaa ttaatgtacg atcaaagtac gcaattgaac gttgatattg
     9061 acggaatcaa aaatgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca
     9121 acaaggcaga ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac
     9181 cgactgaaga ttctgatcta accaacgcga aagtggacga aatcgttaaa gatgtagaag
     9241 acaagggatc taacagtgag tacgttaaga atgcaaccaa taaaattact tcagctgtag
     9301 atgaaaatgg tgagatcact tttgctgatc aaccagccta tgtcaattcc aaagggaatg
     9361 tatatgtggt ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa
     9421 tggttgtgat tgcgccaatg acagacaaca caggatcagg atttttaaaa gatattcatt
     9481 tgtatccaaa gaacattgtt tctaagttga gttttgaatt aacgaaattt ggtgacgatg
     9541 gcacagctca aagcaaacaa acacctttaa aaggggcaaa atttgagtta tacaagggcg
     9601 aacctggaaa aggaacaaaa ttaggagagc ttgtatcgga tgatcaagga aagctgactg
     9661 caactgattt gacgttagga aaatactact ttgtggaagt tccttcagaa gttgtagttg
     9721 gatcagatca agaaccaaca gcagaccaat atttgttagg cgcagacgct cgcaacgatg
     9781 cgcacaataa gttaaccttt gaaattacga atgacggcgt gacaagtgat ttaaaagctt
     9841 cctacgtgaa ctataaagca ccagtgatcg ataaaacagt gacaaatgga acaggagaag
     9901 aacattcttt ccaaattggt gacgcagtga attatcaagg tacaattcat atccctacag
     9961 atatcgcagg tggaacagat gggattacag taaatggtgt aaaaagtgaa acttctcctt
    10021 acagtgtatt taaatggggc gacacagcag gtaaaggact ttcttatgtt gctgaaaaag
    10081 cagatattaa agtaacaaat aatgatggtt cagtcgtatt aaaagaaaat actgactata
    10141 aaatcgaaaa ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa
    10201 gtgatacagt tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga
    10261 atgatagtgc tgaaatcgca aatccattaa caaatagcgt ggatttcgtt tataacaata
    10321 atccatttaa tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg acttatggcg
    10381 caaaattctt aaaagtcgat agcggcctat ttggaacagg tatcaaagca acaccgcttg
    10441 aaggcgcaga atttgcagct aagaatgcag aaggtaagta ctacggtgga cttactgata
    10501 cagataaaga tggtgtcaaa gaagcagttt gggtagacga tgtagcaaat gcagcaatcc
    10561 taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga gattactcac
    10621 tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc tctttcaaag
    10681 tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat caaaaagctt
    10741 ccgtaccaat gactggtagc aacggtttcc aatcttacgt attgatctct tgcttgttgc
    10801 ttggggcagg cgcaatttct gcagttgttt actttaagaa gaaagcctag aaggatcagc
    10861 taatgattat cagacaccct aagaaaaagc gaatcatggg taaatggatc attgctttct
    10921 ggttactttc agctgttggt gtcttacttt tgatgcctgc tgaagcgagc gtggcgaaat
    10981 accaacaaaa tcagcagatt gccgcaatcg atcgaacagg aacggcagca gaaacggatt
    11041 cttctttgga tgttgctaag attgagttag gcgatcctgt tggtattctt acaattccaa
    11101 gtatttcttt aaaactacca atttatgacg gaacttccga caaaatttta gagaatggtg
    11161 tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat cctttaatcg
    11221 cagggcacag tggactttat aaagataatc ttttcgatga tttgccttca gttaaaaagg
    11281 gagaaaagtt ttatatcaaa gttgatggtg agcagcacgc ttatcaaatc gatcggattg
    11341 aggaagtgca aaaagacgaa ctacaaagaa actttgtcac ttacttagaa ccaaatccca
    11401 atgaagatcg agtaacgcta atgacatgta cgcctaaggg gatcaacaca caccgtttcc
    11461 tagtgtatgg gaagcgagta acctttacaa aatcagaatt aaaagatgaa gaaaataaga
    11521 aacaaaaatt atcttggaaa tggcttttag gaagtactgt ttttctttca gtcatgatca
    11581 ttggttcgct atttgtttat aaaaagaaaa aataaaggag ggtaaccaac atgagaaata
    11641 gaaaatggct atttgtttgt gcagccgctc ttttttcttt cactgcctac acacagttga
    11701 cgtttgcaga cagacaagaa agcacggata gcacagaaca aacaatccaa acgtcagata
    11761 gcagcacgat tcaaagcagc gatgaaaccg tcgattcgtc aacagttgat tcttcagaag
    11821 aacctaaaat tgctgaagac gatcaaatag tcaaacagac cattcacgtt caaaaaatta
    11881 tttcttccga acaattagac aaccaggggc aaaagcttga aaaacaagaa ggagtaaatg
    11941 gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat gttaaggatc
    12001 tcactactga ccaaattgaa tcccaattaa aagatcgagt gaaaaaacta tccagtgatc
    12061 aactgaagct aatttctaat ggagaaacaa agacaattga tcaacaaaca ggtgtctttg
    12121 aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc aatgaatcaa
    12181 gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt tctgataaaa
    12241 atggtgaatt cttaaaggat gtgtggattt atcctaaatc agaagcttcg caacctaaag
    12301 aagaagtaaa aaaaatcgta tctactggcg taaagaaaaa cttctttgag aattgttggg
    12361 attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa ctgatcaaat
    12421 tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt ttattatttt
    12481 ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta aacataccag
    12541 ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta tttactacga
    12601 tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag gggatgaagg
    12661 gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca taatgatgat
    12721 gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag ttattgtcga
    12781 tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag atgttcctaa
    12841 tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc atgttgatgc
    12901 caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca aaaatatacg
    12961 ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag gcgtaacaac
    13021 caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga aaaaagtcaa
    13081 tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt ggaatctact
    13141 agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata cgaatggtgc
    13201 aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt tctcaggaat
    13261 tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg acggtagccc
    13321 cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg caggaggaaa
    13381 aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga cagattggta
    13441 tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc tgaacgtggt
    13501 aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt tcaataattt
    13561 gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta aaatatctga
    13621 agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta ctttatcttc
    13681 tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg ataaaaatgg
    13741 aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc gaatcaaaca
    13801 gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg agttaatcga
    13861 taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta caaataaaaa
    13921 attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa aatatgcagc
    13981 gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg aattagtgat
    14041 caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc agggaacgtc
    14101 gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc ctaaaattcc
    14161 agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg gtaagaaggg
    14221 tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca cacaaaaatg
    14281 gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta ttcaagatcc
    14341 aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata agagtaaagg
    14401 aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa cgctgaagtg
    14461 ggaagcttcc gctgacttct taaataacaa tcttttagat ggacgagaaa ttcagctgat
    14521 atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag ccgtagtagc
    14581 agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc ctaacttgcc
    14641 acaagttatt gttcctaaaa caggttctac acatttagta acgattttag cggtcagctt
    14701 actattactt gtgttagcaa cttttggcta cgcagttctg aagttcaatt aaacaaaaaa
    14761 acaaggctga aaatcagcct tgtttttcta tttgaaaatg ggattggcac taataaatac
    14821 gttgtctgaa gaaagtgatc cgtctgcaga aacttcataa gcatgggata aaccaacgat
    14881 actttgattg ttgtaaagca agtttgcgtt atgagcagcg ctatctttcc acataaccat
    14941 agcatttact gctcctgtat ctgaaaggat ctcgtcaacg taaaggtaga aactattgat
    15001 attttctcct ttagcaccgt agcagcctaa gctttctaag agatcttgga aagtaccgcc
    15061 gccaggtcgg taatgactga agcttacttg taattctttt gcacgaatat ctgcagcttg
    15121 ttgaacgata tcattgaatt gcattggggc aacgccattt tgttgacggt agctatttaa
    15181 ccaagcaagt tctttttgac gcacatagtt gtttaaatca cttagtgaga tatttttgta
    15241 tttgaaagtg atcgttcgat tttggtcaac attattgatt acacgttgtc catttttatc
    15301 aacggtaaca gatgaatcgt catttctaca caagaagtag ttttgatcgg tcatattttt
    15361 ccaagaagta gagaaactat ctccctcgta aaccgtttgt gtttcagtag ataaaacagc
    15421 gtttgtgtca acgtttatat ggttcacagt gacgttgtat tgatgagtat aggcaaaatt
    15481 gattccttga ttacttgtaa cgttgtttaa agtcacagtg ttgctagagc tttctctagg
    15541 atcaagaaca aaattagtga acataggtgc ttcaattgtg atgctttgtc cataaacgac
    15601 gggataactc ttactgtaat tatagagttc ttggatattt ggatcagtat ccgagacttt
    15661 atttggacga gctttcactt caactagaaa gacttcatca tagtaaaaag taaatgtggt
    15721 atttgtgtta atcgtttgtg tttgtgtttc attcccattt acttgatact gtggtgcttc
    15781 aaagttagtc aagtcggttc tagcttttgc agtgtaactt gtaccagctt ttactgagac
    15841 atgttcttct tttagcagcg ttccagtttg tcgattcata tgacggacag ttaccgcata
    15901 ttctgaaggt ttaggtttgt gttgtaaccc tttaaatgca gaatcaagac tatttttagc
    15961 attatttact tgggtttgag aagctttaga atcgtctagt acttttttag cattggataa
    16021 agcagtctga aaattattcc aagatccttc tgtaaaatct cctttagcag ttgattttac
    16081 ttggttgtac aaggcttgta attccgtttt gttcacagta ggagctggtt ttttctttaa
    16141 tcccttgtaa gcagaatcta atgtgttttt ggcactatcc acttgttttt gagtcgcttt
    16201 tgagtcattt aacacttttt tagcattgtt taaagcggtt tggaatgcgt tccaggtatt
    16261 gtcggtatag tcaccttttt tagttccctt aacgctcgta taaagagctt ctaaagctga
    16321 tttattcact ttgggtttag aggtgtcagc accataccaa ccaatacctt cgtatttcca
    16381 gccatagtct ttggttaacc attgtacttc tttccaactt gcagtgtagt gatgcgaacc
    16441 agaggtaaca cctggaagga aaaggcggta gatcggttta ttattttttt cggcagaacg
    16501 ccaggcaggc ccttcataag tccaaccgta atttttggtc aaccatttca cttcatccca
    16561 actagctgtg taatggtgat cacgaaggcc tttattgtat aaacgataga caaggtggcc
    16621 accgctagtc ggtgcttccc aagcggggcc ttcataatta ccccacttaa ttttcactag
    16681 gtgatcacgt tcttttgtgc tagaggtgta aaaatgttca tggttatttg gattataaag
    16741 gcgatacatg acgttagttg aagcagcgtc agcagatcca gcaagagcaa ctgtggcagc
    16801 aaatgttcca atagctataa cagtttttgg gtttaataat ttcatgtttt caactcccta
    16861 ttaaatgatt aaatattttg tttacattca taatatacca cggtataccg aggtagtcaa
    16921 cggtataccg tggaaatttt gaaataaaaa agccgcccta tgaagagtgt aaggatggct
    16981 gtttatatgc tatgaagaaa tggattcggc gtatttggtt agtttggcag cattatcaaa
    17041 tcgcattttt tcaatcgcac tttttttctt tattaaatcc gcaattgttg attctgcgac
    17101 tgaagaatgt ttgctaattt catatgctgt cttgccagtt tcaaatagta accattcgat
    17161 tttctcttta tcagcgtaca ttgcaatgct ccctcttgat tttttcttta ttatagctct
    17221 tttttgtaat aaatcacaat atcaagagga aaagtttcta tagaaaggtg ggaaattatg
    17281 agtatccgtt ccccgtgtat caaaatgagg tttgttgcta ctttcgttat ttaatatact
    17341 aaaataaaac aacgaaagaa ggaatagaat gaaaaagtta ataatggtat ttacttcgtt
    17401 gattgccatg attgttattt taggtggatg tggtagtgaa aaaaataagc aagatagtca
    17461 agataatggc gtgttaaaac caggaacagt gtggaaagaa gaagtaggcg gacttgttca
    17521 taatttaaaa attatagatg atacaacatg ggaatattcc gaaagtgttt ggcatcctga
    17581 acaagtacaa ataacagttg agaagctaaa agactataaa ggattagaaa gatataaggt
    17641 aatcgattca ggtggagtag atttattcat aggtaaaaca gccttaatag ttccctataa
    17701 caaagacggt ttagaaacgt ttaaatttat tccaacaaac gaaaaagaac atcgagataa
    17761 aagtgaaata ttaaaagaat acagtactaa atctggatac aagttacaaa agactagcga
    17821 gtgattgcta gtctttttat ttatcctgaa ggaggaatcg cagtggcaaa agctaaaacg
    17881 ccatttgaaa aagccgcgtc taaaatctta gaacgtcgcg gtgtatctga aacacagtgg
    17941 aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga caaggactta
    18001 gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat tattaaacac
    18061 taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta acacaaccga
    18121 aagtagtagc tgcggttgat atttttagag cattacaagg tgcagcaact agtttaaaaa
    18181 acaatctgat gatttttgcg gctgtagctg ccgttgcctc cattgttctt ggggggattt
    18241 tcttcttctt tggccgaaga ggagctgaaa acgggaagat gcacgtaggt tatgtattga
    18301 tgggggtagg agttatatta gctgccgcct ctattgtggt ttcgttattt ggaatgtttg
    18361 gacaaacccc acagggacta taaaaaataa atagaaggag taaaaagtac gtggctactt
    18421 tttactcctt ttttatgaaa ggagaatact catgtacttt acgatttcag gcaataccaa
    18481 aaaagataaa gagttaataa aaaaagcaac gaagaaagtg ggaaagcgta ctgccttttt
    18541 accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg tgttaggaca
    18601 agtgttagaa caagagaatc aatcttctgt taaattgact ctcacgttac atgataaaga
    18661 aggaaaaata gaaaaggctg gcacagaata cgtttttggc ccactggatt atcaaatttc
    18721 agaaggtttt tatcctacat tgatggattt gatccgcctt gatgtgttta aggaaacgga
    18781 tgaaaccatg agtgtggaag agtcttatcg aaacgaaaaa ctgctacaag ttttagaaga
    18841 agagttagag agcgaccaaa gaaaagtgag tcaagtgagt gaagtcgaag taaatgatga
    18901 agtaccatgg gaacgaccaa tctttccaaa gaaggagtca aaagaaaaag agacagtcta
    18961 tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag agaacgatgt
    19021 aatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag agacggccga
    19081 agtagaagtt gatctagttg aagctaatga agagccaaca tggactgaag gggattctca
    19141 ggaatatatt gaaccactta tcagtgaaga agtagagaaa aacgaaacag agatacaaaa
    19201 ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat ggatgaaaga
    19261 aaaaaataaa ttatttttaa atgagcttta ttcgaaatat caattacccc aaacctatca
    19321 agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac ttttagaaca
    19381 gttagagtct gttcaatcaa caaattggca tgagcttgca caagaagatt tattagccac
    19441 ttttgaagaa cgtcaacaag aaacagatca agaaataaat gagtatgcga cggaacagtc
    19501 atctaattgg gagagaacaa aagaggaact aaataaagaa gaacaacgag tgattgaaga
    19561 agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt attccttgtg
    19621 tatagagaaa attaataact ttactaaaac taatcaagaa gctttatcga aagaaaaaga
    19681 aaacttgtta aaagaacgta taaaagaacg aaaagaagag ttctaccgag gtttacgtac
    19741 agataagcta aagattagca aagaattaaa tgaacaactg aataaacttt atcaaacaac
    19801 gactgaagtt cttatggaaa gacacgggca gatccagcaa gaattacaag cacaaattcc
    19861 aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag aagaaaaaca
    19921 acgacaagaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgagc agcatcttgc
    19981 agaacaaaaa gtggcgatag aaaaacaacg gttagagcaa gaaaaggaaa aagaagcagc
    20041 tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagcacagt tgaattatct
    20101 agcacaacct caaccagtag ctgctgttca aacgccagtg aacaaccaag caaatttaac
    20161 tccttcacca cagcgttcct ttaacggatg gatggtggga tgtattgcaa gtttaagctt
    20221 gcttctaggt ggaggaacag tatttgcttt taatcatatc aatcaatctt cacaggcaga
    20281 ttctgtagcc acaacggcgt caatcaagga agaagcccag gaacaagcaa gaaaagagta
    20341 tgaacaacaa ttggaagaat tattgaatcg taccaatcag tcaacagcag agtcaagtat
    20401 ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa ggatcaagcg attcaactgt
    20461 acactcaaac acttccgaaa gttcagaatc aaatgaggtg aaataatgcg tattagagaa
    20521 acaacgagac gaaaagacaa acgattcaat ttagaaaaga ataggtatct ttctcatttc
    20581 tatcgctata ctccttcagg agaagagcta ccagaaaaac atgacaaacg tatttctcaa
    20641 aagatttttt atagtggctt agcttatctt cctttaaact atgttgttac aagaatacca
    20701 gaagcatttc atcgatttga gattattcca ggaagtttac aaggagtgaa tatttttcag
    20761 ttacaagaaa tgccgcagtt attccatttt gattcaactg gtttttggaa tcctttgacc
    20821 ccaacaacaa gtttaggcac acgtttagtt gtttctacga tttgtgttgt tccagctgta
    20881 cttatgggat tgaaaatgga catgtggttt cgtccgctag cagatgggca aaaaggagat
    20941 aaccgattaa aaacgttaaa ggaaatccaa caaatttatc cgcaaatacc agaatcaaag
    21001 ggaagttttc ctggttatgg cggaatacca attactcatt ataaaaaata ctggtatatt
    21061 cagacggata cagtaaatac ttgtattgtt ggtacttccc gctcagggaa aggccagata
    21121 gaagttttag caaccattga caatttgtcg cgtgcagaaa gacaaagttc aatggttgtt
    21181 aatgatccaa aaacagaatt atatgttgct agtaaagatg aactagaagc tagagggtat
    21241 gatgtgtatg cctttaatat caccgatcca ctgcaatcaa tgagtgataa tccattggca
    21301 ctcattgtga aatactggaa gcgaggagac atagacaccg caacacagtt aaccaatact
    21361 tttacgaata cgatttatta tgatgcaaat gcttcagaca ataaatactt taacgaaaat
    21421 gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga tcaaacaggg
    21481 gattactcca aattaacacc aaataatctc gttgaactac tttcagaaat tggcggtttt
    21541 aattattatc cagatcctga gaatgaattc aagcagctca atgccttaga tgagttcatg
    21601 aatcagttac cccctggaaa tatagcaaaa aaacaatatg gggcaacaaa aattggatct
    21661 gataaagcga aagggaatat cttgtctacg gcaattacag gattgaatcc atttacccta
    21721 acgaaaattg cgaagatgac ttctcaatcg accattgatt acaagtccat tggttttccg
    21781 aagtatttgg atctcaaatt acatgaaagc ctattaaatc aacgcattgt cgtggaattt
    21841 tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag ttggctatcg tggtttttgt
    21901 gaaatcaact ttgattgtca tttagagcag ggagatcatg tttgcttaaa atacaatcat
    21961 caaggaaaac aatatgttgc ttggtatgag ttttctcaaa aagtattgaa agacgaagag
    22021 ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt tgccagaact agaaaaagaa
    22081 gccgttctaa cattagatga gaagaaaagt aacctattag tagagtctat tcggatgcac
    22141 tataccgata aaccaatggc tatttttatg ttaacgcctg attatgatcc atctaaccat
    22201 atcattgtta gcatctttct tagtcagttg tataaagaat tatctacgca atgtgttaag
    22261 actaaaggag ataaatgtca ccgtaggatt cattttattt tagatgaatt tggaaatatg
    22321 ccaccgatgg acaacatgga aggaatcatg acggttacgg caggtcgtaa tatgttatgg
    22381 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
    22441 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
    22501 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
    22561 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
    22621 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
    22681 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
    22741 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
    22801 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
    22861 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
    22921 aacgaagaaa atatgtttta taaaatcgtt caatcttttg agaatgagac ggctaaaaaa
    22981 ttgttaattc aattttacga agaacaagca acggatcgtc ttcaagaata tgttgaaaag
    23041 ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac ggctgttatt ggaagctgaa
    23101 aaggcggtga aagaatgatc attggaggaa ttttctctgg catagccgac tggtggtcag
    23161 gtttattcgg agaaggggat gcagatccca aaaccgtaac gaaagtctta caagattatg
    23221 gtaattatct gcagtatcaa gatgtattca gttggatttg taatactttt ttatggggat
    23281 tgatcaaagg gttatataat ctcaatactt tattagaaaa aacaatttat caaagctttg
    23341 atttaaaaga cttattgaac gccgctggtg ttaatgaact atttcaaagt ttgattagta
    23401 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
    23461 gtaaacaccc accgaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
    23521 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaaac ttttatggag
    23581 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
    23641 ataatgtgat tgatgtcagt acattactag cgactgaccc gaataaagta gaatccttac
    23701 caacagataa aagaaatgct ttaacagcga aaaatttcaa aacagcgaat atcaatggga
    23761 ttattacacc tgatcaagct aagaaaatgt ctgaatcaag tgatattagt acagtacaaa
    23821 aagatcggtt aaaggcatta cagtatcgac tagagttaga cgataatggg aaagaaatcc
    23881 ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta tacgtcaggc tatcgtcgat
    23941 acattagtag tccagggatt attttggctg gggaatcttc tttgtcggta gcctatttat
    24001 ttattttgtt tacaatcgtt acttgcatta ttgagctagt atttaaaaaa ttctatttag
    24061 taattgccgc gtcaacggac tttgagacag gacaacggat gaaaagtgcg atagaagata
    24121 tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc tatacattaa
    24181 tgctttcagg tatcggtgat ttgcatgcgg taggaaagat caatggattc ttgtatgttg
    24241 ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt acgaagattt
    24301 tcggtgttga tacgagtctg aaaaacggca gcaattctct tcttagcatg tttgccttag
    24361 gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct ggtaagagtg
    24421 gtttaagcca tttaaatgat attcgtaaga atggatttgg ccatcgaaaa gatattaccc
    24481 atgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat agtaagaaga
    24541 cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca gaaggtcttg
    24601 gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga tcttacagaa aaagccaaag
    24661 acaccttaga agagaaatta gacggcacga gagctaaaga atttgctgaa gctgtgaaga
    24721 atccatccga agcactagaa aaacaaaaag aaaaagcttc agcagtcaaa gaaggactaa
    24781 aagatgcgta tggtgaagga caagtcaatg cagcagtgaa ggcaaataca acaagtgaaa
    24841 aagaaaatca agaaggtgca gcgattcctt cagccaaaga tcaccaagat gagttacatt
    24901 ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg acaacatggt cagttaccaa
    24961 aaaccgatcc agaacaatat cgttataaat ctaacattaa ttctgaaaac gaaaaacgca
    25021 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgatctgc
    25081 caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt ttgcctaaga
    25141 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat gatcaaagct
    25201 ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg cctgaaaatg
    25261 gaaatctgac aactgtttct tacaatttag ctgaaagtgg tgaaacagag aatgcgtccg
    25321 ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat caataaagtt cataatggat
    25381 acagaccagc aaacgtagca tttactgcac cgacaaaaat aaatgtgaca agagaaacta
    25441 aaaacaggaa caatgaattg aaagaacaac tattgaagga tatcaaacca taaaaggcct
    25501 atttaagggc ctttttcttt ataaaacaag tttaaaatgg aaaggagaaa attgtggagg
    25561 atattttaat agctacgttt ataactgcac tgggagcatt tttaacgata tttgtggtga
    25621 ctattaaagg atttaaagat acggatagtt tacaaaaaga tcatcagtct attttagatg
    25681 gtcaaaaaac acataccgaa agcattattg tacacctttc agaaggattc acttatttga
    25741 aagatgctat gcccacattc acagtaaaat taacggaaat atttcattct gttgagcaaa
    25801 tatcgtcaga attgaaagag caaagacaac aaaaagatcg tcaacttggc tcacttaatt
    25861 ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa tcgattaaac gatgaaatgc
    25921 aacgagttca tttggaaaat gaagaattac gcgcacaaaa ttatgattta attcaaaaga
    25981 aaagaaatct ggaacaaaaa attgttgaat taaaagaaga aaatcgtcaa ttgaattatc
    26041 aattacgacg aggaaaaaat cgttctcgtg atttaggaaa tgaattagag ttgtaatatt
    26101 tagaaaaacg ctgtgagaaa tcacagtg
//
LOCUS       DAFBYI010000030        26016 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFBYI010000030 DAFBYI010000000
VERSION     DAFBYI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.030"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LLD04_001056"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LLD04_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK5648002.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LLD04_001057"
     CDS             808..1242
                     /locus_tag="LLD04_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK5648003.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LLD04_001058"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LLD04_001058"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK5648004.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LLD04_001059"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LLD04_001059"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK5648005.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="LLD04_001060"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="LLD04_001060"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK5648006.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="LLD04_001061"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="LLD04_001061"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK5648007.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="LLD04_001062"
     CDS             9513..10439
                     /locus_tag="LLD04_001062"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK5648008.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="LLD04_001063"
     CDS             10621..11514
                     /locus_tag="LLD04_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK5648009.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="LLD04_001064"
     CDS             11648..11773
                     /locus_tag="LLD04_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5648010.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="LLD04_001065"
     CDS             complement(11907..12539)
                     /locus_tag="LLD04_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648011.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="LLD04_001066"
     CDS             complement(12687..13229)
                     /locus_tag="LLD04_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK5648012.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13363..13752
                     /locus_tag="LLD04_001067"
     CDS             13363..13752
                     /locus_tag="LLD04_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648013.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13756..14520
                     /locus_tag="LLD04_001068"
     CDS             13756..14520
                     /locus_tag="LLD04_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5648014.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14649..15596
                     /locus_tag="LLD04_001069"
     CDS             14649..15596
                     /locus_tag="LLD04_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5648015.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15726..16466
                     /locus_tag="LLD04_001070"
     CDS             15726..16466
                     /locus_tag="LLD04_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648016.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16757..17425)
                     /locus_tag="LLD04_001071"
     CDS             complement(16757..17425)
                     /locus_tag="LLD04_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5648017.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17559..17846
                     /locus_tag="LLD04_001072"
     CDS             17559..17846
                     /locus_tag="LLD04_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648018.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18262..19800
                     /locus_tag="LLD04_001073"
     CDS             18262..19800
                     /locus_tag="LLD04_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK5648019.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19797..20762
                     /locus_tag="LLD04_001074"
     CDS             19797..20762
                     /locus_tag="LLD04_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648020.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20749..22071
                     /locus_tag="LLD04_001075"
     CDS             20749..22071
                     /locus_tag="LLD04_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5648021.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22134..22238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22312..23055
                     /locus_tag="LLD04_001076"
     CDS             22312..23055
                     /locus_tag="LLD04_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648022.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23070..24062
                     /locus_tag="LLD04_001077"
     CDS             23070..24062
                     /locus_tag="LLD04_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5648023.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24074..24811
                     /locus_tag="LLD04_001078"
     CDS             24074..24811
                     /locus_tag="LLD04_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648024.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24801..25523
                     /locus_tag="LLD04_001079"
     CDS             24801..25523
                     /locus_tag="LLD04_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5648025.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25607..>26016)
                     /locus_tag="LLD04_001080"
     CDS             complement(25607..>26016)
                     /locus_tag="LLD04_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK5648026.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8951 a         4424 c         5425 g         7216 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaatgataa
    13321 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
    13381 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
    13441 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
    13501 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
    13561 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
    13621 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
    13681 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
    13741 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
    13801 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
    13861 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
    13921 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
    13981 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
    14041 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
    14101 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
    14161 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
    14221 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
    14281 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
    14341 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
    14401 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
    14461 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
    14521 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
    14581 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
    14641 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
    14701 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
    14761 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
    14821 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
    14881 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
    14941 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
    15001 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
    15061 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
    15121 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
    15181 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
    15241 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
    15301 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
    15361 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
    15421 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
    15481 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
    15541 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
    15601 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
    15661 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
    15721 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
    15781 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
    15841 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
    15901 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
    15961 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
    16021 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
    16081 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
    16141 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
    16201 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
    16261 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
    16321 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
    16381 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
    16441 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
    16501 aaggaaacgc tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct
    16561 caaatttttt tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa
    16621 taaaaaaaca aactattttc gcagatcgtt tgcaaaaata gtttattttt ttataaagaa
    16681 gattttactg aataatatca ttttagttta cataatgata tttctgtaaa ttaaataaag
    16741 tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt tcaaatgttt
    16801 ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga tacttcactt
    16861 tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa tgtgtgagat
    16921 ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga cttttgccaa
    16981 tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg cctgtgcttc
    17041 cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc atcaaactat
    17101 tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga
    17161 aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca
    17221 atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa
    17281 aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc ttagatggaa
    17341 tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt
    17401 ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat tttcagcaac
    17461 ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa aattattttt
    17521 ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca gacttattga
    17581 caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg gctcaagaac
    17641 aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt atctttttat
    17701 ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg atgaagaacc
    17761 gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat ggagtgaaag
    17821 agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt tatgatgtca
    17881 aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata atattcgttg
    17941 aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct gtttcttttt
    18001 attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga caatttcgta
    18061 tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt
    18121 gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt ttgttcgtgc
    18181 tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt atacttaaag
    18241 aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga aagaaaaggc
    18301 gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt gctgattgca
    18361 gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc
    18421 ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt aggtttactt
    18481 atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat ttacgctttg
    18541 cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc tattgaagaa
    18601 gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga gcttcccatc
    18661 gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat cggtacggct
    18721 actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt aggtattgac
    18781 cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct tgcaattata
    18841 ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat cattacttta
    18901 gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt gtttaaaaat
    18961 gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat ccaaatgtat
    19021 aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag
    19081 acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc tgagttcaca
    19141 ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca
    19201 aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga tatgacatta
    19261 ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc ttttgcgaac
    19321 gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat caaagcggga
    19381 ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga agcatatggc
    19441 ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc aatcaataat
    19501 ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga gtacgatcta
    19561 gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc gctgatgaaa
    19621 aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa
    19681 ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg
    19741 acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga agatcagtga
    19801 ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg
    19861 acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct tccggtagtg
    19921 gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca ggagacatct
    19981 acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg aatatgggat
    20041 atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat attgaagtca
    20101 tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc
    20161 aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag ttgtctggag
    20221 gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat gtgattttga
    20281 tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa gaattggttt
    20341 tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat atggaagaag
    20401 caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc caatgtgaca
    20461 caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc tttgatgaac
    20521 cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat ttcttaaatg
    20581 aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa gaagtatatt
    20641 cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt attgggtcgc
    20701 tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat gaaaacatat
    20761 gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa
    20821 gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt
    20881 ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa
    20941 ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact tcaaaaattc
    21001 aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc agaagctgaa
    21061 atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga agtcaacgaa
    21121 gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac gatcttacca
    21181 gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc tgtactgcct
    21241 aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac gattgccaat
    21301 gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa agaatttgag
    21361 gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca gtttgctttt
    21421 ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt cggaaaagag
    21481 acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa tacagaatct
    21541 ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt caatgattat
    21601 ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag gaaaacccca
    21661 aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat aacggatgca
    21721 acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag tcctaaactt
    21781 gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt agagatggat
    21841 cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc
    21901 tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc agacgaattg
    21961 atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt ggatagctat
    22021 atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata atcagcatga
    22081 taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat gaatacctgt
    22141 ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca ttgaacctgt
    22201 cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta ctatgtcgtg
    22261 cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa aatgaagaaa
    22321 aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat tgttgtcata
    22381 gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc tgcattagcg
    22441 gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc agtgcttggt
    22501 ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta ttggaagttt
    22561 ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat catggtgtta
    22621 gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc
    22681 ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa agtatcaaaa
    22741 gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta tcgaatatat
    22801 aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc tacttattgt
    22861 gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg gtattacatg
    22921 atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt
    22981 ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta ttatcgtatg
    23041 ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta ttaccgctac
    23101 tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg
    23161 atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat actggcatct
    23221 acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa atcatcgaac
    23281 caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa tttggtgtaa
    23341 gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca gtcaaagcaa
    23401 ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac agtaacatcc
    23461 attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag
    23521 ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg acaatggtag
    23581 gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa tggtactttg
    23641 aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat atcccattga
    23701 aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat gatcaactga
    23761 tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg
    23821 aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca aaagaaaatg
    23881 atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg gatgatcctg
    23941 agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa
    24001 acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag tttataaaat
    24061 aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg aaaacctgaa
    24121 aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga ttgtcgcgcc
    24181 aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc ctatgacttc
    24241 tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat ttagttatat
    24301 gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta cgttatatca
    24361 aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg atatctttgg
    24421 gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga agcagcgaac
    24481 ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg aaccatttgg
    24541 agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc tgcctaaaaa
    24601 agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt atttatcaga
    24661 caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc aagtgcctga
    24721 aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa cgatttatca
    24781 gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat gggagtcaga
    24841 gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt gattcgccag
    24901 aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta agttacggta
    24961 tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag
    25021 caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg atgactacac
    25081 aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa cctgttgtac
    25141 tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac cctaatcact
    25201 atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat ttaggctgtt
    25261 atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta gctaaagcag
    25321 tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag tgggctttag
    25381 gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta aagacaatcg
    25441 ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac
    25501 gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg cacctctttt
    25561 tggctttctt tgtataaaag taaactgttt gattaataaa tgaatcttag taccaaccgt
    25621 ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta acatattgtt
    25681 ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa gaatctaatt
    25741 ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg
    25801 cgatccattc ttttgcagag cttgtgcttg ctggagtagc agcagtttct gttacagcag
    25861 gagcaggttg tgtttcagca acaggcgctg cttgtttttc cgtttgaacc gtttcttgta
    25921 caacaggttg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg
    25981 cttgtgcaga gccttctgtt gggattgtta attgtt
//
LOCUS       DAFBYI010000031        25944 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFBYI010000031 DAFBYI010000000
VERSION     DAFBYI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.031"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="LLD04_001081"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="LLD04_001081"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK5648027.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="LLD04_001082"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="LLD04_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK5648028.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="LLD04_001083"
     CDS             complement(3549..4358)
                     /locus_tag="LLD04_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5648029.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="LLD04_001084"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="LLD04_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK5648030.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="LLD04_001085"
     CDS             complement(7968..8654)
                     /locus_tag="LLD04_001085"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK5648031.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="LLD04_001086"
     CDS             complement(9002..10783)
                     /locus_tag="LLD04_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5648032.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="LLD04_001087"
     CDS             complement(10809..11723)
                     /locus_tag="LLD04_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648033.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="LLD04_001088"
     CDS             complement(11750..12712)
                     /locus_tag="LLD04_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648034.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="LLD04_001089"
     CDS             complement(12712..13656)
                     /locus_tag="LLD04_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648035.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="LLD04_001090"
     CDS             complement(13657..14661)
                     /locus_tag="LLD04_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648036.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="LLD04_001091"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="LLD04_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5648037.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="LLD04_001092"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="LLD04_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK5648038.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="LLD04_001093"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="LLD04_001093"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK5648039.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPPHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="LLD04_001094"
     CDS             complement(18496..20178)
                     /locus_tag="LLD04_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK5648040.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="LLD04_001095"
     CDS             complement(20230..20592)
                     /locus_tag="LLD04_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5648041.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="LLD04_001096"
     CDS             20862..21050
                     /locus_tag="LLD04_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK5648042.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="LLD04_001097"
     CDS             21288..23957
                     /locus_tag="LLD04_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK5648043.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="LLD04_001098"
     CDS             complement(24048..24668)
                     /locus_tag="LLD04_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5648044.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="LLD04_001099"
     CDS             24780..25181
                     /locus_tag="LLD04_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648045.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="LLD04_001100"
     CDS             25316..25654
                     /locus_tag="LLD04_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBK5648046.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4645 g         8773 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg cggcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttcc
//
LOCUS       DAFBYI010000032        25810 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFBYI010000032 DAFBYI010000000
VERSION     DAFBYI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.032"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            9..851
                     /locus_tag="LLD04_001101"
     CDS             9..851
                     /locus_tag="LLD04_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5648047.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            851..1798
                     /locus_tag="LLD04_001102"
     CDS             851..1798
                     /locus_tag="LLD04_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5648048.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="LLD04_001103"
     CDS             1813..2046
                     /locus_tag="LLD04_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBK5648049.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="LLD04_001104"
     CDS             2377..2961
                     /locus_tag="LLD04_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5648050.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="LLD04_001105"
     CDS             3092..3760
                     /locus_tag="LLD04_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK5648051.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3771..5186
                     /locus_tag="LLD04_001106"
     CDS             3771..5186
                     /locus_tag="LLD04_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5648052.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="LLD04_001107"
     CDS             5204..5773
                     /locus_tag="LLD04_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBK5648053.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="LLD04_001108"
     CDS             5883..7637
                     /locus_tag="LLD04_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648054.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7597..9363
                     /locus_tag="LLD04_001109"
     CDS             7597..9363
                     /locus_tag="LLD04_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648055.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="LLD04_001110"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="LLD04_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5648056.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="LLD04_001111"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="LLD04_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5648057.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="LLD04_001112"
     CDS             10381..10758
                     /locus_tag="LLD04_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648058.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="LLD04_001113"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="LLD04_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5648059.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="LLD04_001114"
     CDS             10952..11935
                     /locus_tag="LLD04_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK5648060.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="LLD04_001115"
     CDS             11965..12087
                     /locus_tag="LLD04_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5648061.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="LLD04_001116"
     CDS             12105..13628
                     /locus_tag="LLD04_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5648062.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="LLD04_001117"
     CDS             13652..13891
                     /locus_tag="LLD04_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648063.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="LLD04_001118"
     CDS             complement(14084..14545)
                     /locus_tag="LLD04_001118"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5648064.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="LLD04_001119"
     CDS             complement(14740..14958)
                     /locus_tag="LLD04_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648065.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="LLD04_001120"
     CDS             15076..16692
                     /locus_tag="LLD04_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5648066.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="LLD04_001121"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="LLD04_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="LLD04_001122"
     CDS             complement(18549..18704)
                     /locus_tag="LLD04_001122"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK5648067.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18896..19090)
                     /locus_tag="LLD04_001123"
     CDS             complement(18896..19090)
                     /locus_tag="LLD04_001123"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5648068.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(19000..19332)
                     /locus_tag="LLD04_001124"
     CDS             complement(19000..19332)
                     /locus_tag="LLD04_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5648069.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="LLD04_001125"
     CDS             complement(19846..20037)
                     /locus_tag="LLD04_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648070.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="LLD04_001126"
     CDS             complement(20131..20517)
                     /locus_tag="LLD04_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5648071.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..22416
                     /locus_tag="LLD04_001127"
     CDS             21211..22416
                     /locus_tag="LLD04_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBK5648072.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22433..22867
                     /locus_tag="LLD04_001128"
     CDS             22433..22867
                     /locus_tag="LLD04_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648073.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23484..>25810
                     /gene="esp"
                     /locus_tag="LLD04_001129"
     CDS             23484..>25810
                     /gene="esp"
                     /locus_tag="LLD04_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBK5648074.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8896 a         3700 c         4829 g         8385 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataatatta aaaaaactaa agatttccat ttctttactg gtgattttaa tgagatgttt
    21421 cttctataca tgtttaaaac cagatattac ttatttggtc catttagagc aaataatatt
    21481 gataaagttt tttttaaact aaaaatgaac aatttgaatg ttgcaatggc tgatagagaa
    21541 cgactctata attcattgca aaatttgact ttgtattctt taggagacat tcgagatatt
    21601 ctgattttag ttcattattt ttttactgga aaaatagaag atctattcca tgaaccgtta
    21661 attgaatata cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
    21721 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
    21781 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
    21841 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
    21901 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
    21961 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
    22021 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
    22081 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
    22141 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
    22201 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
    22261 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
    22321 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
    22381 ttaggtttag tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact
    22441 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg
    22501 ggaaatttct gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag
    22561 tgtgttaaat gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc
    22621 tagagaatct atcagtcata ttaatggtag caattttaaa caaacattat ttaatctgat
    22681 taagctagat gctaaaatat caagttattt attctttttg atacatgatg aatttatgga
    22741 ttatcaaaaa ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct
    22801 aactttttca gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag
    22861 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
    22921 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
    22981 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
    23041 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
    23101 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
    23161 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
    23221 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
    23281 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
    23341 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
    23401 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
    23461 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
    23521 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
    23581 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
    23641 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
    23701 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
    23761 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
    23821 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
    23881 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
    23941 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
    24001 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
    24061 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
    24121 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
    24181 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
    24241 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
    24301 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
    24361 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
    24421 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
    24481 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
    24541 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
    24601 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
    24661 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
    24721 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
    24781 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
    24841 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
    24901 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
    24961 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
    25021 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
    25081 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
    25141 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
    25201 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
    25261 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
    25321 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
    25381 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
    25441 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
    25501 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
    25561 caatttcaga tgtctataat aaggacttcg gtaatgcaag tgttgatgca aacactggta
    25621 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
    25681 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
    25741 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
    25801 tcaccgcagg
//
LOCUS       DAFBYI010000033        24665 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFBYI010000033 DAFBYI010000000
VERSION     DAFBYI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.033"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            236..1042
                     /locus_tag="LLD04_001130"
     CDS             236..1042
                     /locus_tag="LLD04_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648075.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="LLD04_001131"
     CDS             1054..2274
                     /locus_tag="LLD04_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648076.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="LLD04_001132"
     CDS             2264..3850
                     /locus_tag="LLD04_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5648077.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="LLD04_001133"
     CDS             3889..6027
                     /locus_tag="LLD04_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5648078.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="LLD04_001134"
     CDS             6315..7706
                     /locus_tag="LLD04_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5648079.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="LLD04_001135"
     CDS             7765..8574
                     /locus_tag="LLD04_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5648080.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="LLD04_001136"
     CDS             8546..9535
                     /locus_tag="LLD04_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5648081.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="LLD04_001137"
     CDS             9649..10644
                     /locus_tag="LLD04_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5648082.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="LLD04_001138"
     CDS             10648..11790
                     /locus_tag="LLD04_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBK5648083.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="LLD04_001139"
     CDS             11787..13214
                     /locus_tag="LLD04_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK5648084.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="LLD04_001140"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="LLD04_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LLD04_001141"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LLD04_001141"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBK5648085.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="LLD04_001142"
     CDS             15286..16677
                     /locus_tag="LLD04_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK5648086.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="LLD04_001143"
     CDS             16810..17757
                     /locus_tag="LLD04_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK5648087.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="LLD04_001144"
     CDS             complement(17797..18957)
                     /locus_tag="LLD04_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5648088.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="LLD04_001145"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="LLD04_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK5648089.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="LLD04_001146"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="LLD04_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK5648090.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="LLD04_001147"
     CDS             21080..21811
                     /locus_tag="LLD04_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK5648091.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="LLD04_001148"
     CDS             21808..22875
                     /locus_tag="LLD04_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5648092.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="LLD04_001149"
     CDS             22882..23514
                     /locus_tag="LLD04_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648093.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="LLD04_001150"
     CDS             23654..24319
                     /locus_tag="LLD04_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK5648094.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="LLD04_001151"
     CDS             24347..24640
                     /locus_tag="LLD04_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK5648095.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8418 a         3663 c         4781 g         7803 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgcttttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gtttt
//
LOCUS       DAFBYI010000034        24304 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFBYI010000034 DAFBYI010000000
VERSION     DAFBYI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.034"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..695
                     /locus_tag="LLD04_001152"
     CDS             <1..695
                     /locus_tag="LLD04_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5648096.1"
                     /translation="VQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDP
                     TRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYQYEYIFIMDYNTGKELAR
                     VNANGVSRPDVNQTYGTYGNVGYHVSFNMRNFPNKKVYVMMRATNDPKGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(802..1350)
                     /locus_tag="LLD04_001153"
     CDS             complement(802..1350)
                     /locus_tag="LLD04_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648097.1"
                     /translation="MPKDLKKTYNQYLTNLDGKTAILTTPFTPLDCFYIKFDILQLPH
                     LLGLHKIYNEPPKSLCSKLASSSITYEKIQRHKMFGSIKDRITLFDFILDIFLEDYNG
                     SVIYVSEADRNGSSMKLDIAFNHPYKNKILTLGLREISPAIYIPVTFYVKKKIIAQDF
                     QQSKRAKILSLEFIGTKNILLR"
     gene            2072..2145
                     /locus_tag="LLD04_001154"
     tRNA            2072..2145
                     /locus_tag="LLD04_001154"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2106..2108,aa:Ile,seq:cat)
     gene            2690..3664
                     /gene="bsh"
                     /locus_tag="LLD04_001155"
     CDS             2690..3664
                     /gene="bsh"
                     /locus_tag="LLD04_001155"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5648098.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            3989..5884
                     /gene="asnB"
                     /locus_tag="LLD04_001156"
     CDS             3989..5884
                     /gene="asnB"
                     /locus_tag="LLD04_001156"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK5648099.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(6079..6525)
                     /locus_tag="LLD04_001157"
     CDS             complement(6079..6525)
                     /locus_tag="LLD04_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5648100.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            6753..6902
                     /gene="rpmG"
                     /locus_tag="LLD04_001158"
     CDS             6753..6902
                     /gene="rpmG"
                     /locus_tag="LLD04_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5648101.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            6990..7508
                     /locus_tag="LLD04_001159"
     CDS             6990..7508
                     /locus_tag="LLD04_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5648102.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(7585..8541)
                     /locus_tag="LLD04_001160"
     CDS             complement(7585..8541)
                     /locus_tag="LLD04_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5648103.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            8707..9513
                     /locus_tag="LLD04_001161"
     CDS             8707..9513
                     /locus_tag="LLD04_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648104.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            9510..10499
                     /locus_tag="LLD04_001162"
     CDS             9510..10499
                     /locus_tag="LLD04_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5648105.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            10496..11500
                     /locus_tag="LLD04_001163"
     CDS             10496..11500
                     /locus_tag="LLD04_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5648106.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            11632..12174
                     /locus_tag="LLD04_001164"
     CDS             11632..12174
                     /locus_tag="LLD04_001164"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK5648107.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            12194..12892
                     /locus_tag="LLD04_001165"
     CDS             12194..12892
                     /locus_tag="LLD04_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK5648108.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            12913..13125
                     /locus_tag="LLD04_001166"
     CDS             12913..13125
                     /locus_tag="LLD04_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK5648109.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            13125..14087
                     /locus_tag="LLD04_001167"
     CDS             13125..14087
                     /locus_tag="LLD04_001167"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK5648110.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            14105..14500
                     /locus_tag="LLD04_001168"
     CDS             14105..14500
                     /locus_tag="LLD04_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5648111.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            14663..15028
                     /locus_tag="LLD04_001169"
     CDS             14663..15028
                     /locus_tag="LLD04_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK5648112.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            15025..16836
                     /locus_tag="LLD04_001170"
     CDS             15025..16836
                     /locus_tag="LLD04_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK5648113.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(16898..17065)
                     /locus_tag="LLD04_001171"
     CDS             complement(16898..17065)
                     /locus_tag="LLD04_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK5648114.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            17313..18302
                     /gene="galE"
                     /locus_tag="LLD04_001172"
     CDS             17313..18302
                     /gene="galE"
                     /locus_tag="LLD04_001172"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK5648115.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            18314..19798
                     /gene="galT"
                     /locus_tag="LLD04_001173"
     CDS             18314..19798
                     /gene="galT"
                     /locus_tag="LLD04_001173"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK5648116.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            19822..20802
                     /locus_tag="LLD04_001174"
     CDS             19822..20802
                     /locus_tag="LLD04_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5648117.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            20993..21715
                     /gene="tsaB"
                     /locus_tag="LLD04_001175"
     CDS             20993..21715
                     /gene="tsaB"
                     /locus_tag="LLD04_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK5648118.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            21699..22241
                     /gene="rimI"
                     /locus_tag="LLD04_001176"
     CDS             21699..22241
                     /gene="rimI"
                     /locus_tag="LLD04_001176"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5648119.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            22248..22712
                     /gene="rimI"
                     /locus_tag="LLD04_001177"
     CDS             22248..22712
                     /gene="rimI"
                     /locus_tag="LLD04_001177"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5648120.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            22709..23725
                     /gene="tsaD"
                     /locus_tag="LLD04_001178"
     CDS             22709..23725
                     /gene="tsaD"
                     /locus_tag="LLD04_001178"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK5648121.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         7753 a         4147 c         5085 g         7319 t
ORIGIN      
        1 ctgtgcagat tgagttacag cacacacatg ataaagcaac ttttgaaaag aactataaag
       61 catacgttga attggctaga gattcagcga tgaaatatgg tattccatta acattggaca
      121 cgccttataa ccaaccagga atcaaatcgc atttatgggt aacacaaaac atctggggcg
      181 atcatacaga tccttatggt tatctttctg aaatgggcgt aagtaaagaa aaattagcat
      241 atgatttagc tcatggattt accgatgaaa atccgacaac ttcagatgat aaaccagtca
      301 ttgatccaac tcgagcaggt gctgcaaatc ctacgctgac agatggaaca aattacgccc
      361 acattgatca gttcggagaa atcgaaaacg caaacttgca tgttgctgga tggcatattg
      421 ctaactatca atacgagtat attttcatta tggattacaa tactggaaaa gaactagcac
      481 gagtaaatgc taatggcgtt tcacgcccag acgtaaacca aacctatggc acttatggaa
      541 acgttggtta tcatgtatcg ttcaacatgc gtaattttcc taataagaaa gtctatgtaa
      601 tgatgcgtgc aacgaatgat ccaaaaggga acactaaagg cggagcacaa gattttcatg
      661 ataaacgctg gtatttaaat attccgcaac gataaaaata gcccctcgat gaggggtggt
      721 acataacgat attgactatt atcagttgtc tttttacgct ttgtgacaac ttgtattggt
      781 tatagtagat aaattctaaa gctatcgtaa gagaatgttt ttagttccta taaactctaa
      841 acttagaatt ttagctcttt tagattgctg gaagtcttgg gctataattt ttttcttaac
      901 gtaaaaggtt accgggatat atatagcggg ggatatttct cttaaaccaa gggtcaatat
      961 tttattttta taaggatgat taaatgcgat atctaatttc atagaagaac cgtttctatc
     1021 agcttcagaa acataaataa cggatccgtt ataatcttct aggaatatat ctaaaatgaa
     1081 atcaaacaga gtaattctat ctttgataga gccaaacatt ttatgtcgct gaattttttc
     1141 atatgtaatt gaagaagaag ccagtttact gcaaagagat ttaggaggtt cattatatat
     1201 tttgtgtagt ccaagcaaat gtggaagttg aagaatatca aattttatgt aaaagcaatc
     1261 tagaggggtg aatggggttg ttaaaattgc cgttttgcca tcaagatttg ttagatattg
     1321 attataagtt ttttttaaat ctttaggcat agaaggcgct cctctgttta gtttttaaca
     1381 aaaaaaaaga gagtgcaaag tatggatacc gtcccataca gaccaacgct aggctaaccc
     1441 gcctgcgggg gaacgtgagt gcactcttct cactttgatt gatatgatta ttatcaatct
     1501 agagctaact gaaagatgag gtgatagatc tatcctcgta attaatataa catttacgta
     1561 aaatttatgc aagcgtaaaa tataaaaaac aataagatga aaaattaaga aaacttaata
     1621 tatacgattt aatataacta tttgtacgtg tggcacatta ttttgatgca atataattac
     1681 aaattataag tgataaaata acgttttttt gaatattgta attcttgcta ataaaaaacc
     1741 attgatctcg gaaatcaatg gcaaggttat agattctaga cactagaatt caagactaag
     1801 ctagggattg aacagattgg atatccgcgc aagtgcttgt aggccatgtt gacagcatgg
     1861 tctttttttg cttacaatgt cattctatac tacgtaaaaa aagcgtcaat aattttttta
     1921 ctataaagct aagcaaatga atgttaaata tactctattt tttcttttag agttatgcaa
     1981 taataaatat gccatcacaa caaagaatga aacccattat tatctagtct atctccattc
     2041 tttttgttag caggagttgt ggtggctttc cgtaccctta gctcagttgg ttagagcaga
     2101 cggctcataa ccgtccgatc gtaggctcga gtcctacagt gtacatactc taagtgattt
     2161 aaattgcgaa taaaatataa aaatgactcc ccttttgact cccccaagca gtatatggat
     2221 gtctctaaaa gttgtttttt tagataataa aaagctgata ataagcgaaa aacacttaaa
     2281 atcagcttta aaccctattc tcgaaatccg gcgaaccggc taataccggc gcgcaggttc
     2341 aaatcctgta ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa
     2401 gtaataagaa cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt
     2461 accgcagatg caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt
     2521 tgtcacagcc ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta
     2581 ctatttttta taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaatac
     2641 tagatgtaag ataaaagtgt aaaaacgatt tgttttagga cgggaaacga tgtgtaccgc
     2701 aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta
     2761 taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa
     2821 tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta
     2881 ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc
     2941 tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg
     3001 gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct
     3061 agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga
     3121 taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc
     3181 tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta
     3241 tcgctcgcta tcaaatggaa caccagaaaa tcatttttca aaccagatca gcttgaatgt
     3301 ttatagtcgt gggatgggag gattgggctt acctggagat ctctcttctg tttctcgatt
     3361 tgtaaaagcc acatttacta aaatgaatgc tgcatcaggt gattcagaat ctgaaagtat
     3421 cagtcaattt tttcatatac taggctcagt cgagcaacaa aaaggagtat gcgatactgg
     3481 agaaagaaaa tacgaatata cgatttattc ttcttgctgt aacgttgata aagggattta
     3541 ttattatcgt acgtatgaag acagtcaaat tactgctgta aatatgaata aagaagactt
     3601 ggatcgtcat gaattggtca gttatccagt cataaaagag caacagataa actatgtcaa
     3661 ttaattaatg aatagatccg tttggtatca tattttctga tacagtcata attagacacg
     3721 atctttaggt cgtgtctttt tttgctggtt tttttggaaa tagcatccac taatattttt
     3781 ttaacacgaa gagaataagg tagcctcaaa aaagaaaaca tggtaaatta gatattggga
     3841 ctttattttc tgtaaaaaca gaaactttca tcttttgttt tcacgtgacg aaattgtaag
     3901 agagaaaata ttcgtccgct atttattcta tgataagatg catatgaatg aaataaacag
     3961 taattttata ttaggatgtg aaagttaaat gtgcggtatt gtaggttttg ttaacgacaa
     4021 ggacaacaaa aaaacaatta ttaatacaat gatggatcga atcgttcacc gtggacctaa
     4081 tagttcagga gaatatatcg ataaacatgt tgctttagga ttcagaagat taagtattat
     4141 tgacttagag ggcggtactc agcctattta caatgaagat cggacaaaga tcattatttt
     4201 taatggcgaa atctataatt accagccttt gagagaagaa ttgattgcag ctggccacgt
     4261 ttttcaaaca catgcagata cagaagtcct tttgcatggt tacgaagaat ggggaacaga
     4321 attattgcaa aagatccgcg gaatgtttgc ttttgctatt tgggataatg aaaagaatga
     4381 gttattcggt gcaagagacc actttgggat caaaccatat tattatgcag aaatgaacgg
     4441 aacatttatg tttggttcag aaatcaaaag ttttctgcct catcctgatt ttaacaaaga
     4501 attgaacaaa gaagcgttga aaccttatat gactttccaa tattctccat taaacggcga
     4561 aacattcttc aaagatgtct atcgtttgcc tgaaggccat tattatacgt ataaagatgg
     4621 aaaaatagac attcagcaat attgggatgc agattttgaa acaaaagaaa cgcattcccg
     4681 acaagaatgg atcgaaaaaa tcgacgaaac cgttcaagct tctattgaag cacatacggt
     4741 cagtgatgta gaagttggtt ctttcttatc cagcggggtt gattccagtt atgtaacctc
     4801 tgtattgaaa ccagatcatt ctttctcaat cgggtttgat gataaaacct ataatgaagc
     4861 gattgaagcc agaaaactaa ccgaactatt ggacttagat aatacagcag cagtgatcga
     4921 cggagacatg tcgttcaaag cattcccttt gatccagtat cacttagatg aacctgattc
     4981 aaatccttct tgcgtacctt tgtacttttt agcaaatctt gcttcacaaa gtgtgcgggt
     5041 agtccaatct ggagaagggg cggatgaact gtttgcggga taccaaacgt atggattcca
     5101 tacgaattca aaattcatcc gtgtgattgc tcaaggattg aaaaaattac caaaaggaac
     5161 acgctataat ttaggacgta aaatcggaaa aatgaaaaat ttccatggac ggatccattt
     5221 atatgaatca cttgctcctg caaaagaata ttttatcggt catgctcgag tgttcgaaga
     5281 atcagaagca tcagaagtgt taacaccaaa atatcaaaca gcaccatctg tggatgaaat
     5341 catgactgtt cattacgaga aaacagaagg aatcaaagat gaagtcaata agatgcaata
     5401 tgtcgatctt catcaatgga tgccaaaaga tatcttgtta aaggcagata aactttctat
     5461 ggcaagttct ttggaagtcc gagtaccttt attggatatc gaagtcatga aattggctca
     5521 acaaatacca agtaagtttt tattgaatca aaacaacaca aaagatattt tcagacaagc
     5581 tgcgaacaaa catcttccgg aagaatggtc gaatcgtgtg aaattaggat tccctgtgcc
     5641 aatcaaagct tggttgaaag aagaacacgg ctacgaacaa gtcaaagcat tattcgaagc
     5701 agattttgca aaagaatttt ttgatcaaga aaaaatcatg aagttgctag atgaccatca
     5761 cgaaggacgt aaagaagagc aaagaaaaat ctggacaatc ttcagtttct tgacatggta
     5821 taaagtctac tttgtagatg aaacgattcc gcaagcagaa gcaatcgact atgtgactgt
     5881 ttgatgaaaa tcaaatatca ataaaaaaga ggttttctcc taccgtttaa cactgagtga
     5941 taagaaacaa tttcaaaaaa cagctcttcc cgttttatag atattatgac ttatggttcg
     6001 gagttggcag aggaacacca ttgtttcgaa cataaggggg ttgtgacacg attttcgtca
     6061 caaccccttt acatatgttc aataatcgat ttctttttct tttgtcccat cttttttata
     6121 aatgaacaaa gttgattctt tgttttgtgc tacttgcttt cctttttcga ttgcttcttt
     6181 ttttgtatca aaatggttgc ttgccttttt tgccccttta gatttcacga tccattgttc
     6241 ttcctcatat tctacgatca catctgaatc aagcagtttt ccagcacgag gattcgtatc
     6301 gtgttcatcc gtcttgcttg gatttttttc tttttcgaaa gttcgttttt ctgattcact
     6361 agcatcttca taccattttt ttgcttgatc aatggcaatt gggatcgcac ggttatcagg
     6421 gtacccttca tctaataatg catttgcgat gtcgattgct ttttttcttg tcaatttatc
     6481 taagtttttc attgatgcag gaaagtcctt catgttccat ggcatattag tatccccctt
     6541 tattaaaaaa cataatctat ataaacaagt gtaagggtat ttatacaaaa agaaaaagat
     6601 tttgtttgag aagtaaaacc gttgtattta tcgtcaagta gttttactta acaaaatcgt
     6661 gaaaaacact agcaatctag aaaaaatcat ggtaagataa tcaagtctga gagtcatctt
     6721 agaggattct tgtaaaaatg ggagggaaaa acatgcgcgt aaacatcact ttagagtgta
     6781 cttcttgtaa agaacgtaac tatctaacaa gcaaaaataa acgtaacaat cctgatcgtt
     6841 tggaaaaaca aaaatattgc ccacgcgaac gcaaagttac tttgcaccgt gaaacaaaat
     6901 aatgttttta aagttctcaa ttccttgctg gtcaaagaat tgagactttt tttatctcaa
     6961 aacatctaaa atcataagga gcggataata tgtttatttc catgtgggca caagataaaa
     7021 atggtctgat cggcaaaaat ggtttgttac catggcgatt gccaaatgat atgcgtttct
     7081 ttcgcgaaca cacaatggat cgaattttag tgatgggaag aaagacctat gaaggaatgg
     7141 gggatctttc acttccttat cgccatatca tcgtgttgac aacgcaacca aattttcgca
     7201 cgaaagaaaa tgcggaagta atgcattcga tcgatgaact tttgtcgtat agtcaaacga
     7261 tctcagaaga tatctatgtt tcgggtggta gtcggatttt tcaagaactt cttccttaca
     7321 ctggtcggat ctggcgaaca ttgatcgata gcacgtttga aggcgatacg tatattggga
     7381 atattgattt ttcagacttc gcccttatag aggagtatga ggggataacg gatcaagaaa
     7441 atctatatgc ccatcgtttt caaaaatggg aaagagtaaa aaatagggtt gcttcaaaag
     7501 aagaataaaa tctatgcgtc tagtttgtta ataagaaaaa aagcatctca cgactaatcg
     7561 tgaagatgct ttttttagtt aaaatcaatt tagtttttct ttgatatcat tgaccatttc
     7621 ctgactggca ataggtccgt tatatagcca actagattca ggtccgaatt gcagtacatg
     7681 attgtctgtc actgctttta gattcttcca gcttggttca ttgaacatgg cagcatcttt
     7741 gtcactgttc acaaggatga tgtagtcagc atctaattca gctagctttt cagaggaaac
     7801 agcagaccag tcagaagtgg cttttttgga gacctcttcc actagatcag gaacaccaaa
     7861 ttccagatcg tcgtatacga ttcttccact tgaccgattt tttctgacca taaaggctga
     7921 gttgttggtc acccacaaca ctgcagctgt cttgttctcg attttatctg ctagttcttc
     7981 tcgagttttc ttgatggatt tttgataatc ggaaatgatt ttttctgcct ctttttcttt
     8041 tgccaaagct ttgccgacat cttttagttg ttcttcccat gtgacatctg ttccgttttt
     8101 tacaacataa gttggcgcga ttttgctata ttgttcgtat ttcccgcctt cgacagtagc
     8161 ggaagaagaa atcaacagca aatcaggctc aaagcttaac actttctcat aaggaaggtc
     8221 ataagaaatc gttgggacat cctttaaatc atcttgcaga taatcttgaa tggaaccact
     8281 gccaacagtc cactgggcga taggtttttc ccctagtgca atcagatagt cttctaagta
     8341 actgccgatg atccgtttgg gcgcgtttgg aatctctact ttatgtccaa gtgtatctgt
     8401 gactgtatga gtactattgg atgaagtcgt cgaattggca gttgtagctg tttctttatc
     8461 tgaagagggg gagcaagcgc tcagtactcc agccagcagc aatatgctag ataatgtgaa
     8521 tacttttgtt tttaatttca tttctttcct cctaacataa ttgagaatct ttctcattgg
     8581 ttattattcc tgatatttcg aacgctgtca accgcttcat ttggctatag atatgaaagt
     8641 agcgcttgac gaaatgttga aacagcgtca taattgagaa tgattttcag cgaaaggatg
     8701 ggtgaaatga cgagattaag agcagctgac attgcagccg gctacgaaaa caaaaaaatc
     8761 attgaaaaca tgtctgttac gattcctgaa gggaaaatca ctagtttgat tgggccaaat
     8821 ggaagtggaa aatctacttt attgaaaagt tttacccgta tactacctgt gaaatcagga
     8881 aagatcattg tcaataactc tgatatctct acatacagtt caaaagaact tgcaaaaaaa
     8941 attgcattat tagcacaaac tagtgaacac cctacaggta tgacagtaga agaagtagta
     9001 tcttacggac gataccctta tcaaaaactg ttcagtggca tggacacgca agatcatgaa
     9061 gcaattgagt gggcgttgga tgctacaggt ctagctggat ttcgtatgcg ggatatcgca
     9121 acgctgtctg gtggacaaac ccagcgtgtc tggatcgcta tggcgcttgc tcaggaaaca
     9181 gatatcttga tcttggatga accgacaaca tttttagatc cggcacacca attagagatc
     9241 ctacagttat tgactgaaat caatcaaatg aaacaaacaa cgatcttgat gtcgattcat
     9301 gacattaatc atgcttcacg tttttcagat tatttattag caatgaaaaa cggtaagttg
     9361 atcacagaag gtacacctga agaagtgatc acaaaagagt gtatgaaaaa aatatttgag
     9421 attgatgcaa cgattgtcac gcttccaaga acaaataaac cagtttttct aacctatgat
     9481 ttgttgccaa aagagcagga ggatcaatta tgaggactat gaagaaaaat agactcctgc
     9541 ttttggtctg ttttgttctt ttgattattt tttttgttgc tctttcaaat ggcgcagcga
     9601 ctgtgggaaa agcagatttt tggcaggcgt tgtttgattt tgatagatca aaacaaagtc
     9661 accaaatcat acgaaatatt cgtttgcctc gtgtggcggg agcatttctt gtagggagtt
     9721 gttttgcatt gagcggtgca ttgatgcaag gagtgacaag aaatccttta gcagattcag
     9781 gcttactagg aatcaacgcg ggtgcttcgt tggcaatggc ccttagtttt gctttttggc
     9841 ctaaagcttc tgatttttct gtgtttctaa tgtcattggc aggttctttt gctgtaactt
     9901 tgctcgtatt tggttttttg cagttttcga aaatgggcat gaatccttta cagctgattt
     9961 tagctggcgt agctattagt tcttttttta ctgctatcag ccaagcactt acccaattct
    10021 ttaatctgca gcaggatctt gctttttggt ttgtaggcgg agctgccaat atcacatgga
    10081 aacaattagc agtattgttt cctttatata tacttgctgt tttggtttct ttttcattag
    10141 gaaaagcaat atctttgatt catttgagtg aaacacatgc attagctctt ggaagtcatc
    10201 ctaaacgaac gcggttgatt gttttctgtc tagttgcagt gttagcagcc attgccgttt
    10261 cgttagtcgg acctgtttca tttattgggc tgttggttcc gccagttgtt cgcggaatgg
    10321 ttggaacgga ctatcgttat gttctacctg tctctttttt agttggcggt ggattggtta
    10381 tgttggcgga ttttacagcg aggatgatca atcccccttt tgaaacacct tttggattag
    10441 tcattgcatt gattggcgtt ccgtttttac tgatacaagt aaggagggaa caacggtgaa
    10501 aaaaatatat tggacgttct atttcttgct ttttgtactt gtcatcctgt ctataggtag
    10561 tttaatgatt ggtacgcctt ttatttacct ggatcagctt tttgccacgt tcatagggaa
    10621 agaatcatct tctttggcgc tgattgttag agaatttcga gttcctcgat tactgatcag
    10681 tttattagca ggagcttgtc ttggcgtttc tggttatatc ttgcaaggag taacaagaaa
    10741 tgaattggca gattctagta tattgggcat taatgccgga gcaggatttt tagtgatgct
    10801 ttatttaggt ttcttttcgc actattcgct gccgtttcta ttgctagttg tagcctttct
    10861 aggtggtgtt ttagctgctt gctgtgtcta tcttgcggcc tatgatcgaa atggcttttt
    10921 gggtatgaat cgaatcttat tgtccggtat tgccgttaat gccggccttt ctgcattaac
    10981 attgctatgc acgatccaat tatctaagga aaattatgga tttgtgaatg catggttagc
    11041 gggatcaatc tggggtgcga gctggcctta tgtttgggca ttgcttccat gggcggttat
    11101 cttaattccg ctaggaggac tttatgcaca gaggattgta ttgctatcat ttggtcaaga
    11161 acgcgcgcaa ggattaggcc ttaacgtggc taaaagtcaa aaaatccttt tgttgttagc
    11221 tgttgctttg gcaagcggta gtgtagcggt gacaggtagt ttatcttttg taggactact
    11281 agctcctcat gctgcgaagc tagtcgtacg taaagaaaat aactggacct ttgttctgag
    11341 cggtttgttc ggttccattt ttctgcttag tgcagatatg gttgctagag tgattctact
    11401 aagtggggaa gtacctacag gaattttgat tgcccttttt ggtgcgccat attttctgta
    11461 tgcacttttt atcagacaaa aacacgcgct gaacagttga aattcattaa aaaaggaagg
    11521 ccaatcattt ctaggtcttc tttttattct atttatgcat ttatttgaaa gttcgattgc
    11581 tatttttctt tttttcctat accattttat gagagaagga agggtgagtt tttggaccaa
    11641 ttgaaagtaa gattacgccg gaaaggaatc gaaaagctga aaaatctggc caaaagtggc
    11701 aagaaaaagg aaaaagaaca gcgaatctat aaacagctat tcgccagcga gtactggaag
    11761 tcagcaaaag tgatagggac gactttatcg aatgaatttg aactggatac acagccggtt
    11821 atccaacgag cagaaaaaga acaaaagaaa atagttgtac ctaaaacttt gccgaaacgt
    11881 cagatggcgt tttatgaaat ggatgaacag tccgttcttg tacgttcgag ttttggtgtt
    11941 cttgagccac aatcagagaa gctctatttg ccggagatga tcgatctcct cattgtacca
    12001 ggtattatct acaatagagc cggttatcga atcggttttg gcggtggtta ttacgaccgg
    12061 tatttaaata attttcataa aaaaacatgt agccttgttt ttcgtgaaca attgaatgaa
    12121 caatggcaac cggagagctt tgatcagaag atccaacaat tgtttattga ctaaaatgaa
    12181 atgggaggtt ttcatgaatt atcaacagca aataaaattg cgtgcatggt taagacgacc
    12241 gttatggacc tatgcttttt tagggataca gacagcggtc ttcctcatga tggaattatt
    12301 tccgcgactt gagatccctt attatgccgg aatgtatggt ccttatcttg ttcacttcaa
    12361 tgaatggtgg cgtttagtga caccaatctt catccatttt ggactgatgc atttcgtaat
    12421 gaactcactg atactgtatt ttatggggca gcaaatcgag gcaatctatg gccattggcg
    12481 ttttttcttg atttatatgt tcagcgggat aatgggaaat acagcaagct ttgcttttaa
    12541 cgaagcgaat gttctgtctg gtggtgcaag tacttcgata ttcggtttgt tcggtgcgtt
    12601 gtttatctta gggtttcatt ttaaacataa cccagcgatc cagcagctag tcagacattt
    12661 tcttttattt attgtgttga catttgtttt tggtcttctg gatacctctg tagatgtttg
    12721 gggacatgtc ggtgggctaa ttggcggatt agtgttaggg aatgttttgg gtctaccgaa
    12781 acaacagaca agctattcga tacaccaaag gattctttct acactagttt ttgtatttct
    12841 ttttgtaata tgtattttac tgggtttaaa aaaatatggt ttacttgtat aatatgaagg
    12901 aagtgcttaa agatggagag cttatatgat gtacagcagc tgttcaaaaa atttggcatc
    12961 tttatctatg tgggtgcacg catctatgac attgaattaa tgactatcga attgcgtaat
    13021 ctgtatgata gccgtttgat tgatcatgat acatacatga cagcgtggcg catattaaaa
    13081 agagagcacc gaattgaaga aagccgaaag aaaggaaatt tgtaatggac aagaagatta
    13141 ttgggattga tttaggtgga acaacagcaa aatttgcaat tttaacacca gaaggagaga
    13201 ttcagcaaaa atggagcatc gataccaata ttttggatga tggtaagcat atcgttcctg
    13261 aaatcattga atcaatcaat catcgtttga atttatataa tatgaaagcc gaagacttta
    13321 tcggaatcgg catgggtacc ccaggaagcg tggacagcga agctggaacg gttattggtg
    13381 cgtataactt aaactggaca gaagttcaat ttgtgaaaaa actgatcgag gcaggaacag
    13441 gaatcaaatt cgctatcgat aatgacgcga atgttgctgc tttaggtgaa cgatggaaag
    13501 gtgctggaga aaacgatcca gacgttgtat tcgttactct aggaacaggt gttggcggag
    13561 gtatcgttgc tggcggaaat ttgattcacg gcgtgagcgg agctggagga gaaatcggac
    13621 atatcactgt ggatcctgaa gggttcgaat gtacttgcgg taaacgcgga tgtttagaaa
    13681 ctgtatctag cgctacaggt gtggtacgcc taggtcgttt cttggcagaa gaatacgcag
    13741 gggattcaaa actgaaagca atgctagaca acggagaaga agtaacaagt aaagatatct
    13801 ttgaaatggc tcaagaagac gatccgtttg cattgatggt tgttgatcgc gtatgcttct
    13861 acttaggttt agcttgcgga aacttgggga atacattaaa tccgtcaagt atcgtgttag
    13921 gtggaggagt atcggcagca ggagaatttt tacgtagccg tgtagagaaa tattttaaag
    13981 aatttacttt cccgcaagta agagaatcaa caaaaatcaa attagcagag cttggtaatg
    14041 aagctggtgt cattggtgct gcctcattag ccttgaaatt tgcttaaaaa aacggaggca
    14101 ttacatggtt ttatgggtaa ttaatatcat tttgctattg attgtgcttg caatcatcct
    14161 ttgggaagtc tatctacgag taatggccaa acgttcagca acgactttga cagaagaaga
    14221 atttcgagaa ggaatgagaa aagcacaagt aatcgatgtt cgcgaaaaag atgtgtttga
    14281 tgctgggcat attttaggtg caagaaacat cccttacact gttttgaaag attcattagg
    14341 atctattcga aaagaccagc ctgtctatat ttacgatcaa aagaaaagtt tgagcatccg
    14401 tacagcaaat aaattacgta aagcaggtta tcaagagatt tatttactta agggtggata
    14461 tgacggatgg tctggaaaaa tcaaaagtaa aaaagtataa aacagatgat tgtatgacag
    14521 ctttaaaaac cgaatcaatc attagatttt gaccaagatc acatgaacgg cgcaatggat
    14581 agttgtgccg ttcatttttt ttattttgtg ctattatcta catatataag ctaagcgaaa
    14641 aggtagaaag aaggaaacaa ctatgatcaa attaaaacgt gtttaccttg atgctgaaaa
    14701 agaggatggc tatagaatac tcgtggatag attctggcca cgaggaattt caaaagaaaa
    14761 agcgcagtta gatctgtggt tgaaagaaat tgcacctagt aatgaagcac gcaaaacttt
    14821 tgaccatgta ccagaacgat ttgcttcatt tgcaaagaaa taccagcaag aattgatttt
    14881 cgaagaagaa aagaagcaag ctatttttaa actcagtcaa cttatgaaag aacatccagt
    14941 cattactttt gtttatggtg caaaaaatga acatcagaac catgctgttt tattgaaaaa
    15001 atttatcgaa atggaggcat gcaaatgaaa atagggatca ttggtgccgg acctagagga
    15061 ttaagtgtag cagaaaggat tctccataat aatcaggaca gacattctct tgaacttttt
    15121 cttttcgatc ccaatggtcc tggaggtcga gtttggcggt tgaatcagcc accggagtta
    15181 ttaatgaatt ctgtttctca acaagtgacg ctttttacag atgaaacatt gtccagtgga
    15241 ggattggtaa cacagggacc caacttgttt caatggagta aaggacctgc tacagattat
    15301 atcgaacaga cggtaaccaa aaataagcaa ctaattatag aagaaatcaa tcgtctggaa
    15361 aaagatcagc aatctactcg atgtttatac ggactctatc aacgctggtt tttctcaaag
    15421 ttgcaagaag aatttccagc ttctattcag tttgtttatt ctttagtaaa aaaagtaagc
    15481 aaaagaaaag atggttatct tttacagaca gatcagcaaa gctattctat cgatcagctt
    15541 gttttagcaa gcggtcactg ggaaaatgat gagacagaag aagaaagagt gtgggtagac
    15601 tacgcaaaga aaaatgtttt actgtatcag cttccttcta atcctgcaga tgtagatctt
    15661 tcgaagatac cgcctaaaga aaatgtcttt ttaagaggtc taggcttaag tttttttgat
    15721 tatgtcggat tgtttacagt atcaagagga gggcgcttcg agaacgaaaa gggaaaactg
    15781 gtctatcatc catcaggtaa ggaaccgatt gtatacagtg gatcaagaag ggggctccct
    15841 tattaccctc gaggaagaaa tcaaaagcaa ggcggagcaa tggcatggcc acggttacta
    15901 acaaaagaaa acctggagca atggcatcag tcgggatttt tgactggcga aacatttttt
    15961 gactatctga aaaaagacgt tgagttgttt tattacaaga aaatcatcga agaatatcac
    16021 ttgccgattc caaagaaaaa ttttgagcat gattttctct ccctctcttc agaggagtgt
    16081 ctcggaaagc atccagcact aatcccttat agatgggatt ggtcgtattt agaaactcct
    16141 cttgcctatc agaatgagcc cttttcagaa gctagccgaa tatttattga agggcagata
    16201 aaagaagcgg aaaaaggcaa ttgcacagga gcatttgctt caacatttga tgcattaaaa
    16261 gactggcgtg atccaatccg gcaggctatt gaatggcacg ttttttctgc taaggaatac
    16321 aaagaaatac tatggggatg gtttactcat ctaaatagct ttttaacgat cggtccgcca
    16381 atcattcgga cgaaagaatt agcagcactg atagatgcgg gtatttttca tttgatcgaa
    16441 ccgccatttg atattcagca aaagcacaac cgctatgtta tttctctaca agataaagaa
    16501 atcagcagtc gctatttgat tgaagctcga ttgccacaaa caagcttttc tcgttcaagt
    16561 aatcctgtca taaaaaattt gaaggaaaac aaacttgttc gccctttttc attttcagat
    16621 aatacaggaa gttatcattc agaagctata gatgttgagc gaggtacgaa tcgcgtgatt
    16681 gatgcttcag gggaaatcca gtctgacttt tattgctatg gaatacctgt tgaaggtgtg
    16741 gattggctaa ctgcagcagt agctagacct tatacagatc catggaatct gagacaggca
    16801 gataaaatag cacaacttat attgactgac aaataaaaat acgcatgatg acttatctgt
    16861 tttttagaaa gtcttcatgc gtatttttaa ataattatta acgagaaacg cgtttacgca
    16921 ttgctttttt acggttttct tcgatcattg cttctttttc gtcaattgga tcgatcactg
    16981 tttttttcac agaagcaacc aaaccagcaa cggcagcggc tgttgccaga cttcctacta
    17041 atattccaga tacaaatttt ttcattgaat ccacctcatt tattatttgt tatatctatt
    17101 atggaacaaa agagaaaaaa aggctagttt tacgtttgtt tgtaaacctt tgcaaaaaat
    17161 ccttgaaaat gtttactaaa tgttttatta taaatttacc atatgaattt acgaaatttt
    17221 actttgtttg ttttcaccat aaaagtaaac ttcgtttttt aaattatata gaatcaacaa
    17281 acacaatttt actaatcaaa ggagtgtttt ttatgacaat tttagtacta ggcggagccg
    17341 gttatatcgg ttcgcacgct gtagaccagc tagttcaaaa ggggtatcaa gtagctgtgg
    17401 tagataattt gctcacaggt cacaaacaag cagtacatcc agatgctcat ttttatgaag
    17461 gagacatccg ggataaagaa tttctacgtt ccgtatttga aaaggaaccg attgaaggag
    17521 taatccattt tgctgcaagc tctttagtag gagaatctgt cgaaaaacca ttgatgtatt
    17581 tcaacaacaa cgtatacggt atgcaaatct tacttgaagt catgcacgag ttcaacgtga
    17641 ataaaatcgt attctcttct actgcagcaa cttatggaga gccgaaagag tctccaatca
    17701 cagaagatac accagctaat cctaaaaatc cgtatggtga aagtaaattg atgatggaaa
    17761 aaatgatgaa gtggtgtgac caagcatatg gcatgcgcta tgtcgctttg cgctacttca
    17821 atgtagccgg agcaaaagca gatgcatcta tcggagaaga tcatacacca gaaacgcatc
    17881 ttgtcccaat cattttacaa gtagctcttg gtcaaagaaa agccttggct gtttatggag
    17941 acgattatga tacaccagat ggaacatgta tcagagatta tgttcaagta gaagatttga
    18001 ttgctgcaca tattttagct ttggaatact tgaaagaagg aaatgaaagc aacttcttca
    18061 atttaggcag cagcaaagga tactcagtca aggaaatgct agaagcagct agagaagtga
    18121 caggaaaaga aattccagca gaaatagcac caagaagagc tggagatcct agccgattag
    18181 tcgcttcaag tgaaaaagcg agagagattc tgggctggaa acctgaatac acagatatca
    18241 aagcgattat caaaacagca tgggattggc atgtcagcca tccaaatggt tatgaagaat
    18301 aggggaatga gatatgtcaa tcagtcaaac gatttgtgat tttgccacac ttgcgatcga
    18361 agctggcgga tggatggaaa tggaccgctt atatttacag aatcgtcttt tagggatggt
    18421 aggagaagat gctctagaaa gcattgaagt aagaccagtt aaggaagaat ctgtagactt
    18481 attggatcag cttgtcgcta aggcaagaga aaatggtgtt ctttcagaat tgccagcaga
    18541 acaagagatt tttgaagcgc aattaatgga ttttctcaca ccgccacctt cagtcgtcaa
    18601 tgcattcttt gcacagcact attcgaaaag cccagaggaa gcaactgagt atttctttaa
    18661 tttaagtaag aaaaatgact acatcaaaac aagagccatc gcaaaaaaca tcaaatttcc
    18721 ggcagagtcg gagtatggtg aactggaaat cacaatcaat ttatctaagc cagaaaaaga
    18781 tccaaaacaa atcgcagcag aacgcgaggc ggtaaaagta gactatccta cctgcatgct
    18841 ttgcatggaa aacgaaggat atagaggtcg cttgaactat ccagcccgca ccaatcaccg
    18901 gattattcga atgaatctag acggtgaaag ctggggattc caatattccc cttatgctta
    18961 ttacaatgaa cattcgatca ttttatcaga acaacaccgc ccgatgaaaa tcacaaagga
    19021 aacatttgct agactgcttc ggattactga agtcttgcct cattactttg ttggttcgaa
    19081 tgctgactta ccaattgttg gaggatcgat tctttctcat gatcattatc aggcaggacg
    19141 tcatgaattt cctatggcaa aagcaccgat agaaaaactt atcaaactag aagattatcc
    19201 gcaagtcatc gcaggagtcg tgaaatggcc gatgtctgtg atccgtttgc aatcaacaga
    19261 gaaaaacttg cttattgaag cagctaactc agtattggaa aaatggaaag catattcgga
    19321 tgaaacagta tctatcagag ctttttcagc agatggtaca ccacaccata cgattacgcc
    19381 aattgcacga agaagggaca attactttga gttggacctt gtccttcgag acaacaatgt
    19441 ttcagaagaa catcctgatg ggatttttca tccacataaa gatgtccaac atatcaaaaa
    19501 ggagaacatt gggttgatcg aagtaatggg gcttgctgtt ttaccacctc gtttgaaacc
    19561 agaactagta gaagtagagc atttcttatt aggagaagaa aaccagatgg ctgaatatca
    19621 tcgtccgtgg gcagaacaat taaaaaaaga acatcctgat ttgacgaaag atacggtcac
    19681 cgatgtcgta caaaaatctg taggtcaggt ttttgcacgt gtactggaag acgctggagt
    19741 attcaagcga gatgaaaaag gacaggcagc gtttgaacga tttactgcga ttttataaac
    19801 gtaaaaaagg aggagagaca aatggcaaca attaaagaca ttgcgcagtt ggcaggagtt
    19861 tctcctgcta ctgtttcaag agttctcaac tatgatcctg atctttccgt tggcttagcc
    19921 acaaagcaaa aaatttttga agcagcagaa cgcttaaatt atacgaaaca tcgtaaaaat
    19981 gcagcaaata ctaagcaaac gcttcgcctg atccagtggt acgacgatac ggaagagctt
    20041 gaagatcttt attatttatc tattcgccta ggaatcgaaa aaaaggcaga agaagcaaac
    20101 gttactttat tgaaagaaac gtgggattcc attagtgatc ttccggcaga tggaacgatc
    20161 gcactaggaa aattcgacga agctcagtta ttagaaatga aaggatccca aaatgccttg
    20221 cttttggtag attctgacgg cacttctcaa ggaatcgact ctctagttgt cgactttaag
    20281 tcaagtgttc aaaaagtcat tgaacacttt gtctcccata aagcgcaaca tatcgctatg
    20341 cttgctggaa cagaatatac gaagaaaaac catcagcggc taaaagatcc cagaattatg
    20401 gcatttcaac aagttttggc agaaaaaggc tacgacgaag gtccaattat cgaagcagat
    20461 ttttctgtcg agtcaggata tcaagcagtg aagcagtatt taaaaacgaa tccgcatgtt
    20521 cctgatgcgc tttttgcagc taatgatgca ttagcgattg gcgcactgaa agcgattcaa
    20581 gaaaaaggtt tagtggtacc agaagatatt tctgtgatgg gcttcaatga tgtcagtgta
    20641 gcgaaatatg taacgccctc gctttctact gtaaaagttt ataccgaatg gatgggagag
    20701 ctagcagtgg agacgattct tcaattaagt aattccaaag caccggtttc aagaaagatt
    20761 attgtggaaa cagagttgat tattcgagaa tcaacgaaat aaaagatgga aaagctgtgg
    20821 gaaaatggag tgataccgca gcttttttgt gtttcaggaa ggggcaaaat ttgcccgcca
    20881 gcagataatc atgtattatt gtactagttt tgcctttggt attttcatcc tcagaataaa
    20941 aaagtatgct acaatggaag gcgagaatgt catagcagaa aggatcaaat atatgattac
    21001 attaggaata gatactgcaa accaaacttt ggcgatcggt gtggtagaag acgaacaaat
    21061 tcttggacaa atacaaacaa atatcaaacg caaccacagt gtaacactga tgcctgcaat
    21121 cgaccaattg tttgcagatc ttcaaataag tccaaaagac attgaccgaa tcgcggtttc
    21181 tgatggtccc ggttcttata cagggcttcg tataggtgta acgacagcaa aaacgattgc
    21241 ttatacatta gataaagagc tagtaggagt atctagtctg aaaaccatcg cggctaattg
    21301 catcggtgtg gataatatta ttgtgcccat gtttgatgct cgaagaaaaa atgtatacgc
    21361 tggggcatac cgctggaaag aaaatagatt agaaactgta cttgaagatg tacatatttc
    21421 tgcttctgat ttgttcacac aactacaaaa gctgaatgaa gctatttatt tcgtcgggag
    21481 tgattgtcac aaattcttct ctgaaataaa ggaaatattg cctagtgcga tgattaatac
    21541 agttccagca tgggatattc caagtggtgt aactgtggct caattaggtg ctttagttga
    21601 accagttgca gagaaacaag cttttcttcc tcgctattta aaacgagtag aagcagaaga
    21661 aaaatggttg gagacgcaca cacctggaga cgaaagttat gttgaaaaaa tttagagcgt
    21721 atgccagaag cttttttcaa gataaaatga tttatacaga aaaaacagtc gaacaaatcg
    21781 atcgtcgtta cgtactaaga gaattgacta cagaagatat caaagagctg ctgggtgtcg
    21841 aacgagaagt ctataatggc gaattgccat ggacaaaaag tgcctttata tcagaattga
    21901 agtcaccgct tcctcatcta tatctaggta tctttgataa tggaagaatg attggtttta
    21961 tcggttcaag agcgttcggt ttagattgcc atatcacgaa tattgccgtt cgaccggcat
    22021 atcagcgtaa acgaatcggt tcgctgctta ttgacgaaat cgagaacttt gctataatga
    22081 atcgctgtga gacgatgtca ttagaagtgc gtatgagtaa tcaagatgca caaagacttt
    22141 atcggaaatt ggggtttcaa gcaacaacga tcaaaaaaag atattacact gaaacaaaag
    22201 aagatgcgtt ggatatgata aaacttttag aaaatgagtg agattagttg atttgcagaa
    22261 aaaaagattt tgatgaagcg tacttggcag aatgcgtatg gatgattagc gaaaatgctt
    22321 atccacatgg ttcaccttgg acgaaaatgc agtttttggc agacatccaa cagcctcact
    22381 ctgattatct gcttttagct gaagataacc agatctgtgg ctttattgga tactcgaaag
    22441 taatagatga agtcgagatc acaaatattg ctgttgcagt ttcagaacaa ggaaaagggc
    22501 atgcacggca tttgttacaa ttactgatta cggaacaaat aaaagattcg ctgcatgttt
    22561 ttttagaagt acgtctatct aatgaagcag caagaaaact ttatgaatca gaaaagtttc
    22621 gtatattagg taaacggaaa tcatattatc gcaatccgac ggaagatgcg atcattatga
    22681 gcagaaagtt aaaaacggag acgatgaaat gaatgaagaa ttgattctgg cgattgaatc
    22741 aagttgtgat gaaacgagtg tagcggtcgt tagaaatgga acagagatct tatctaacat
    22801 cgttgcttct caaataaaca gtcataaacg ttttggtggc gtagtacctg aagtagctag
    22861 ccgccatcac gtagaacaaa tcaccttatg cttggaagat gcactggtag aagctggtgt
    22921 gtcggcagaa gatttatcgg cagtcgctgt cacttatgga ccaggacttg tcggctcgtt
    22981 gctgattggt atttctgcag ctaaggcttt tgcttgggca caccaattgc cgcttattcc
    23041 tgtaaaccat atggcagggc atatctatgc agcccgtctg gttaaaccat tccagtttcc
    23101 attgatggca cttcttgtta gcggaggaca tacagaatta gtctatatgc aagaagatgg
    23161 atcatatgaa atcattgggg agacaagaga tgacgcagca ggagaagctt atgacaaagt
    23221 tggaagagta ttaggtcttt cttacccaag cggaaaagaa atcgatcagc tagctcatca
    23281 gggaaaagat aattaccatt ttccgcgagc gatgatccat gaagataatt atgatttcag
    23341 ttttagcgga ttaaaaagtg catttatcaa tttggtgcat aatgcacaac agcgtggaga
    23401 agatctagac aaaaatgatt tagcagctag ctttcaagca agtgtgatcg atgtattgat
    23461 tactaaaaca cttcgagctt gtcaaaatta tccagtcaag caactgatta tagctggagg
    23521 tgtagcggcc aatcaagggt tgcgtgaagg attgcaggat gctttggcaa ctaaactccc
    23581 tgaggtagaa ctagtgattc cccctcttcg actttgtgga gataatgctg caatgattgg
    23641 agcagcagca catgtggaaa tgcaaaaaag aaattttgct agctatcagt tgaatgctga
    23701 tccaagcttg gttttatctg aataaagagg ttgtgaaaaa gctgccctgc atcaagtaat
    23761 aagaacagcc caggaaaaac ggcttctcat gtttttgacc tggctgttct tattaccgcg
    23821 tatgcaagct tttgaacgcc gtttaatcaa gagttcgtaa gcctgtcgtt taactctgat
    23881 tcacaaggaa caatttcaaa aaacagctcc tcatgtttat gaacattatg acttgtgacc
    23941 gaagagttga caggtacaca ccgtttaatc gaaatagggc tgtgacacgt ttttgtcaaa
    24001 gccctatttt ttacatactt ttaatcatta ttgttatttt gctatttttt acagcttatt
    24061 tatcattatc cgttcctctg attgccctca ctgagtaatc gtttattgtc gaggaatatc
    24121 gaataatcta gatcggatga ggacaagacg cttcatctag ttaatctaac agtatttttt
    24181 gcaaacagtg aatattacat aactgaaaca cacatagaaa atctttgagt cttgtccagt
    24241 gatctaaaac tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat
    24301 attt
//
LOCUS       DAFBYI010000035        23970 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFBYI010000035 DAFBYI010000000
VERSION     DAFBYI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.035"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..363
                     /locus_tag="LLD04_001179"
     CDS             <1..363
                     /locus_tag="LLD04_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBK5648122.1"
                     /translation="MMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGV
                     LGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIR
                     MEIDQLNRQQRLFGDDNE"
     gene            541..882
                     /locus_tag="LLD04_001180"
     CDS             541..882
                     /locus_tag="LLD04_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648123.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            875..1546
                     /locus_tag="LLD04_001181"
     CDS             875..1546
                     /locus_tag="LLD04_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK5648124.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1552..2244
                     /locus_tag="LLD04_001182"
     CDS             1552..2244
                     /locus_tag="LLD04_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648125.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            2252..2767
                     /locus_tag="LLD04_001183"
     CDS             2252..2767
                     /locus_tag="LLD04_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5648126.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            2876..3727
                     /locus_tag="LLD04_001184"
     CDS             2876..3727
                     /locus_tag="LLD04_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK5648127.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPPKKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYQKKKALERAN
                     RQSKAPF"
     gene            3739..4008
                     /locus_tag="LLD04_001185"
     CDS             3739..4008
                     /locus_tag="LLD04_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648128.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFADCVKGLQKHLSELEQV"
     gene            4013..4177
                     /locus_tag="LLD04_001186"
     CDS             4013..4177
                     /locus_tag="LLD04_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648129.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDE"
     gene            4170..4472
                     /locus_tag="LLD04_001187"
     CDS             4170..4472
                     /locus_tag="LLD04_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648130.1"
                     /translation="MNKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEGSS"
     gene            4469..4630
                     /locus_tag="LLD04_001188"
     CDS             4469..4630
                     /locus_tag="LLD04_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5648131.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            4627..4932
                     /locus_tag="LLD04_001189"
     CDS             4627..4932
                     /locus_tag="LLD04_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648132.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            4932..5288
                     /locus_tag="LLD04_001190"
     CDS             4932..5288
                     /locus_tag="LLD04_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK5648133.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            5275..5493
                     /locus_tag="LLD04_001191"
     CDS             5275..5493
                     /locus_tag="LLD04_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648134.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5490..5906
                     /locus_tag="LLD04_001192"
     CDS             5490..5906
                     /locus_tag="LLD04_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK5648135.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVSVSVRNGFDYLDNKVCIVKNSIGHSGLVCAT
                     VDEDLEYRIFNTELFEEYTYEVIGNIYENSELLEEK"
     gene            5906..6199
                     /locus_tag="LLD04_001193"
     CDS             5906..6199
                     /locus_tag="LLD04_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648136.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIKS"
     gene            6229..6669
                     /locus_tag="LLD04_001194"
     CDS             6229..6669
                     /locus_tag="LLD04_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5648137.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            6743..7210
                     /locus_tag="LLD04_001195"
     CDS             6743..7210
                     /locus_tag="LLD04_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBK5648138.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTANHDFEAERMLIDKEEAQEEFEYLHKLFIRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            7535..7678
                     /locus_tag="LLD04_001196"
     CDS             7535..7678
                     /locus_tag="LLD04_001196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648139.1"
                     /translation="MRIGNVYLISKVPYQKLLYSCKVRFIASDLRNLRKQFILSDADE
                     LAD"
     gene            7983..8288
                     /locus_tag="LLD04_001197"
     CDS             7983..8288
                     /locus_tag="LLD04_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648140.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            8269..8655
                     /locus_tag="LLD04_001198"
     CDS             8269..8655
                     /locus_tag="LLD04_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648141.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREAE"
     gene            8652..9032
                     /locus_tag="LLD04_001199"
     CDS             8652..9032
                     /locus_tag="LLD04_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5648142.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIIENETEDKKVFPSYFN"
     gene            9144..9596
                     /locus_tag="LLD04_001200"
     CDS             9144..9596
                     /locus_tag="LLD04_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBK5648143.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDFLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            9593..11320
                     /locus_tag="LLD04_001201"
     CDS             9593..11320
                     /locus_tag="LLD04_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5648144.1"
                     /translation="MIEPGVNYADLFAKEVRKRPKKYPKTVRLAIDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGDMVGKPFILEPWEKFIYSWIYGWVRENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGALGYAMYGEGALSVNCYACASDFAQAQYAAQPFADSILN
                     NPVLLDGTKIFKGPKGTVSSITYEYLDGDLALKNKFIVQTKNIENIEGANPYFVCNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSS
                     FVLIYEPNKGYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            11384..12571
                     /locus_tag="LLD04_001202"
     CDS             11384..12571
                     /locus_tag="LLD04_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5648145.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQDDSNHSVKM
                     IPLGKGYTIETLKSPVDDQAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            12543..13112
                     /locus_tag="LLD04_001203"
     CDS             12543..13112
                     /locus_tag="LLD04_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK5648146.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWDDL
                     SEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            13125..14498
                     /locus_tag="LLD04_001204"
     CDS             13125..14498
                     /locus_tag="LLD04_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5648147.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAV
                     VDELNGIKSELSEADGSDEGSDADEGTGGSDGSTGSDENRSGEDNDQEEDSDSEDSSE
                     NRSGMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALG
                     IEAGNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKE
                     RSTDIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEIN
                     YMFNGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGR
                     FIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPV
                     LYFGDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYE
                     ITKPVGG"
     gene            14500..14778
                     /locus_tag="LLD04_001205"
     CDS             14500..14778
                     /locus_tag="LLD04_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK5648148.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            14759..15085
                     /locus_tag="LLD04_001206"
     CDS             14759..15085
                     /locus_tag="LLD04_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5648149.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            15075..15413
                     /locus_tag="LLD04_001207"
     CDS             15075..15413
                     /locus_tag="LLD04_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648150.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            15403..15768
                     /locus_tag="LLD04_001208"
     CDS             15403..15768
                     /locus_tag="LLD04_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5648151.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKRKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            15775..16401
                     /locus_tag="LLD04_001209"
     CDS             15775..16401
                     /locus_tag="LLD04_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648152.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFGKFIAQPVYDEEQWKTLAKVGGAG"
     gene            16401..16865
                     /locus_tag="LLD04_001210"
     CDS             16401..16865
                     /locus_tag="LLD04_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648153.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            17060..19369
                     /locus_tag="LLD04_001211"
     CDS             17060..19369
                     /locus_tag="LLD04_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK5648154.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLGELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRRGIGGKVSGGNIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            19381..20085
                     /locus_tag="LLD04_001212"
     CDS             19381..20085
                     /locus_tag="LLD04_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648155.1"
                     /translation="MNPDEPNFIWKKRNAVIDMDCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKV
                     SGKFKEVRFWNRSVYL"
     gene            20082..22904
                     /locus_tag="LLD04_001213"
     CDS             20082..22904
                     /locus_tag="LLD04_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648156.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRGGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNKNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRTAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKSGYKTATRGTLWYTGSMAADAR
                     GPRQYLTEISPNKAKAGDIIIVNQGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            22915..23433
                     /locus_tag="LLD04_001214"
     CDS             22915..23433
                     /locus_tag="LLD04_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK5648157.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTK
                     YEIKVRHGNVFSAAEIGRAYPWALYLWTVIVHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            23399..>23970
                     /locus_tag="LLD04_001215"
     CDS             23399..>23970
                     /locus_tag="LLD04_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5648158.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYKDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESI"
BASE COUNT         8368 a         3814 c         5311 g         6477 t
ORIGIN      
        1 atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa
       61 gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa
      121 gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta
      181 ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta
      241 cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc agaaactgcg
      301 atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga tgacaatgaa
      361 tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga aatactataa
      421 accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa
      481 gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat
      541 atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg gggcaagccg
      601 aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta
      661 gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt ttatcttatc
      721 catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga tggcttgatt
      781 gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt
      841 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
      901 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
      961 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt
     1021 aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag actttcttgt
     1081 tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac
     1141 ggttaaagga cacacggaaa ccgaattatt tcctgtttta gattatcgaa accaaccagt
     1201 tgttaaacca aatgcaatgc aaatcaataa ctcattgaag cgatgctttg tgaaagcatt
     1261 ggctctacac ggactgggat tatatgtatt tcaaggggaa gatattccaa caccacctag
     1321 aatcgataca aagaaattaa acatgctaga gacgattcta gaagctttca atgagcagat
     1381 gggtaaagat atgaccaaaa ccttaatcga atatgttaat gagcagacag ataaattagg
     1441 gctcttagct gataacgttg aaactattga acagttaagc tatgagcaat gtgccttgat
     1501 ggagcgagca atagcagcta agagaaaaga attagataag aagtgatatg agtgtttaaa
     1561 ccattaatcg attcatattc agcggttctg aaaaagttca aaggaaaaga cataagcgca
     1621 accatcaatg aggaagtgaa cattgatcga ctaaagacga tgtatgacgg ctacgatggt
     1681 gatcgagtca ttgaaattcg ttttattgat cctagacgtt tcaccgtaca gcaacgaaac
     1741 ttcatctatg cgctgatagg cgatattttt atcgatacag gcatgccaac ggacttctgg
     1801 aaggaattct tctactttcg tttcgaaggt gtcacagggc gcaaaataag cctcaaagat
     1861 gaatcgagca caaccgtaag tgatgccaat atcttagcga atatcatcct agatttcatc
     1921 tttgagcatg atattccgtt taaaaatgga tatgaaattt tgccccaaaa cgaacagtat
     1981 ttcttttaca aatgcctcac gaacagggtg tgctgtagtt gcggaaagaa aaatgctgat
     2041 atacatcacg tagactctgt cggcatggga aataaccgaa aaaaaataaa caattctggt
     2101 aggcgcttta tggctctgtg cagagaatgc catacgaaaa ttcatgtcga aggttttact
     2161 acatttacaa ctaaaagaaa gcttacggca gttgttctca aagacagtga tttaaaaaga
     2221 ttggggttga atatcattga atgaattatg gatggatatc gaaggatacg agggagtata
     2281 tcaagttagt agcttaggaa gagttaaatc taatcgccct aactatgtaa ttaataaggg
     2341 atacgttaac aaagaaggat ttattatgaa accatctgac aatgggaaag ggtatcaaat
     2401 tatcttctta agcggcaaag gttttagaga tagaaggtat gtccatcggc tagtagcttt
     2461 tcacttttta aaagaagcgt atttcgaagg tgctgaagtc aatcataaga acggagataa
     2521 atctaacaat actgttgaaa acctagaatg gctcagttct gatgataata aaaagcatgc
     2581 tagaaaaaat ggattaacta acttgaaagg accatcaaaa ctaactgatt tgcaagcttt
     2641 agctatcaag cgtttatata caaacaaatt gatgagctcc ggagaaataa tgaaactttt
     2701 taatgtcagc aggcataccg tgctgaatat agcttcaggg aagacattta gtttcttgga
     2761 agattaagag aaacacaaaa taggtgtgac taatttcaaa aataagtatc aaatcaaagg
     2821 gattaagtta aaccaggaaa caattaagaa acttagaata ggaggataaa aaatattggc
     2881 tgacaacaaa cgatactact atttaaaact aaaagagaac ttcttcgata gcgacgagat
     2941 ggttctttta gaaagtatgc cagatggata catttattct aatattcttc tcaaacttta
     3001 tttaagaagt ctgaaacatg aaggcaaact aatgtttaat gacaggattc cattcaattc
     3061 tacaatgctt gcgactatta caagacactc tgtgggagtt gtagaaaaag cagtacaaat
     3121 atttcgtgat ttacaactta ttgaggtatt agataacgga gcaatttata tgtctgatat
     3181 acaaagtttt attgggaaat cttcaactga agctgatagg aagagaaaat acagaaaaga
     3241 aattgaggaa gcaaaacaga atttaataac aagtggacaa atgtcggaca aatgtccgga
     3301 caaaactaca ccagagttag agatagagtt agagaaagat atagagttag agaaagatat
     3361 agagaatgta acgcctccga aaaaatcgaa ggctaagccc atccgtcata aatacggaga
     3421 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
     3481 tgactaccaa gagcgaatag aacggctatc tgagtattgt gaatcatcag gtaagactta
     3541 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg agcctaagaa
     3601 tgcaagtggt gcatacaaac gcacaggacg acgagagaag cttccagaat gggcaatcga
     3661 ccaagaagcc tatcaaaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     3721 attctaagag gtggaaaatt gaaaatcgat tatctagaac tgatcaatga aatagcaagt
     3781 tacaaaactg gtgaggaaat agagattctg agagacgttt atgaacaact tgatgaagct
     3841 ggaatcgaac gaattaagaa tgatcgttca agttggagta aactcagata ctatttcgca
     3901 ctttatatcg atgcaacaca attaagaaat ttagcttata caaaattact atttgctgat
     3961 tgcgttaaag gattgcaaaa acatcttagt gaacttgagc aggtgtaatc agatggatct
     4021 aaaaacattt acagcacaga tcgaactaat gcatcaagaa gctttaagac aaagcgcctc
     4081 gtacgaagac aagtggctca acacgttcca tggtggacgt gagagcgcac ttgatcaagt
     4141 gctcaaatta ttgaaaggag aatgtcggga tgaataagaa agcggcaatg cagcgaatta
     4201 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     4261 gacttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccgcga aaaatcgcaa
     4321 tttggcacgg tgacaaactt ctagtgacag ggacagctga acagttagca agtctcacag
     4381 gcttgcacga gaaaatcgtg agaaaaagag ctaggtgtgg atacacagac gttaagaaga
     4441 gaacgtttag atacgtggag ggatcgtcat gacaacagaa gaagtgattc aaatgcgtat
     4501 tcgaaacatt cagcgtgaaa ttgacgatct agaacgaaca aaggcagtga tggtcaatga
     4561 aacggctaga aaggcaatcg atttgcacat agagaactta agaagggaaa ttcggagatt
     4621 ggaggaatga gcgtggataa gaaagcagca atgaaacgaa tcatcgaact gacacattct
     4681 gagaattggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     4741 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     4801 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     4861 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     4921 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     4981 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     5041 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     5101 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     5161 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     5221 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     5281 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     5341 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     5401 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     5461 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     5521 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     5581 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     5641 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     5701 tatcagtcag cgtgcgaaat ggattcgatt acttagataa taaagtttgt attgtcaaaa
     5761 attcaatagg acattcggga ttagtttgtg ccactgttga tgaagattta gagtatcgaa
     5821 tttttaacac agagctgttt gaagaataca cgtatgaagt catcggaaat atatacgaga
     5881 atagcgagtt attggaggag aaataatgaa actaaaagac ggattttacg ctagtagtca
     5941 tggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac gtaagaaacc
     6001 aaaagtcaaa gtcggtgaca tggttcgctg tgaagcagag gagttcatct atccgtttcg
     6061 tggatatgta gagcatctct ataatcactc agcaatcatc cgcattgaaa acacgatgga
     6121 atgtgacaag tggttagcga aaagcaaaga gaatttagct gtagcgagat tggtggatat
     6181 ggaagttata aaatcttaat tttgcttttt tacaactatc ctacaacaat gagcgttatt
     6241 ccacaactgg aaggagaaga ggcaattgtg aacattttag agaatataga tattaagcaa
     6301 acaaggaaaa atgctagacg attactaaaa agatacagaa atttagaacg tttggtcgga
     6361 ccagtgaaaa tagacttttc cgtgatgact gttaccaaaa atttgaaatt cacaattgac
     6421 agtcaaaacg aagaaatcat tgaagcgata agtactcgag attcggttat cgaagcatta
     6481 acacggctaa gtagaatcca tttccaggta ctttattata gttattgttt tcctaataag
     6541 atgtcgatgt atcaaatagg agaaaaatta ggatattccg atagaactat cgagagaatg
     6601 aaggcagtag ctttggttga atttgctgag gcgtataaat caggagaact catttcacgt
     6661 acaaaataaa aaagccggat cgctccgact atgaataata tttccgacat aagtattata
     6721 tcataattgg gggaatcaga ggatggtact tttcgatgta aagaaatatg agacaccaag
     6781 cgcgaaagat gttgatatgg aacgcacaaa acataatgtc gctgtgtttc tttcagcata
     6841 tctatcagct agatgtagag tagggcaacc tcgagaacca aaagtgacag catcatactc
     6901 cttggttcca ccatctacag ctaatcatga ttttgaagcc gaaagaatgt tgattgataa
     6961 agaagaagca caagaagaat ttgagtattt gcataaattg tttattagag gatactctgc
     7021 tatacagcat ccgcacaaac ccgatgtgac tgaaaggcgc aagaagatat tctatgatcg
     7081 ttatatcaat ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc
     7141 ggttaaagta gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc
     7201 tttcaagtag ccagttttta cactttttat acccttttat taccaatttg gtttccattt
     7261 tatacctttt ttataccaat cacttaccta ttcaatgttg tattatgata gtgtcgaaag
     7321 attagtgata ggtctgagac aaaataataa taaaaggaac atcgttttat tattgtttca
     7381 caattaagct tcgatagata gcgacggaaa tattaagaat aaggatgtga attccaactc
     7441 cttctaaatt gttcttatta tctatcatcc gttgctgtct attgtcatta tgtcattgtg
     7501 gcggaaaggg tagacgcatt gagggagaga gtacatgcgt atcggaaacg tatatctgat
     7561 atcaaaagtc ccttatcaaa aattattgta ctcatgcaag gttcgattca ttgccagtga
     7621 cttaaggaac ctacggaaac aattcatctt atcggatgcc gatgaattgg ctgactagtc
     7681 gggatgctac tagcagttag aaggcataaa atactagcgc agacgtgcgc cactctcagg
     7741 tgtaggttag gagagaaaca ttagttgggg ttattaggaa tacgataacc tgcttgcgac
     7801 aaagctttgt actgtcgcgt tggtcatgaa cagagacggt attctgtttc agtattcgtt
     7861 agcaaccgag ggatgtggca gtggtgaggc gcaggaagta ttagacttgt ctgtgtgtag
     7921 gttgctatta catattagat cactctttga gtggtctttt tattttgaaa ggagttttat
     7981 ctatgaatga ttttcatgag gcaatgctta tggcgaatgt cccaacgaat atgggagatg
     8041 tctataagaa agcaatagaa gcagagaaca gtcctactgg tttacgaaac aattggaacg
     8101 gcacctatgc aaatgtgcaa gtagataaag ataatcgtgg gatagcaatg gatgtgttgg
     8161 ttatttcttt attatcccac actttaccca atttgcagga aactgttgaa tggtatgaac
     8221 gaatgggtgc aaaagtaatt agcacaaatt acaaaggaga gaatcaaaat ggcaatgatc
     8281 aaaattagaa caaccatcac aggaacagag tattgggatt cagaaaagaa aaagactgtc
     8341 gtagttccga aaggtcaaga acctgatttt gaagcaacgg aagaaaaagg aatattaagt
     8401 gatggtaaaa cgtttgtggc tgttaatggt gaacttatta ctaatagcag tgaaattctt
     8461 gatagcgatg gaaacactgc tgctgatttt gatggagacg aagctactaa tgatcagtcc
     8521 actgaagata cggatgaact ggacaacatg actgcaaaag aattgcgtgc atatgctaag
     8581 aaacatggta ttgatattcc tggtgctatc cgtgcaaaag gcgatatcat aaatcttatt
     8641 cgtgaagcag aatgaaatac tgtcagtttg acggatgcac gaacaaaata gcaaagggta
     8701 tctactgtac tgaacacaag agatcaagca gatcacgcaa gaagaagcag caagcaaagt
     8761 ctgtttatca tcatgagaac aaaccattct atcgaacaca ggcatggaaa gatatgcgtc
     8821 aatttattta tgaaagagaa ggtggtcact gtcagcgatg cggtcagttc atctttggca
     8881 agagggcaca cgtccatcac attgtaccga tcaaagacaa tgaactgctt aagcttgatc
     8941 caaacaatct catgctttta tgttcaaaat gtcatccaat tattgaaaat gaaacggaag
     9001 acaaaaaagt tttcccttcg tattttaatt gaagcccccc tatccatttt caaaattttt
     9061 tcgcgtgggg agatagggta gcggggagtc acgcgcatcg ttaggtcaaa tttttcaaaa
     9121 aacaaagggg ggtgtataca agtatgacga ctaaagcgca acgtaaagcg attgttgatg
     9181 aaaaagtaag tgctgaaaaa gctcgtattt taaaaataat gaatttgtct gatttgtaca
     9241 ccatcactct tgatccatta atcgaatcat atttggatat ttttgaaatt taccaacaca
     9301 aatatctttt gtggaaggaa aaaggctttc cggagacgca aaaattcacg aataaatcag
     9361 gtgccactaa tcaatcgaag catccattgg cgcagcaagt agaaacttgg tcagataaga
     9421 aaatgaaagc tctagatttt ttggggctaa cgaataaggc taaaactggt agacaaataa
     9481 ctggcggatc gactgcaaga aaagatgaag aaattacacg tccagaagaa aagccagtag
     9541 atgaactagc agcgcacaga aacaaatggc gtaagaaggc aggtactgaa aaatgatcga
     9601 acctggtgta aattatgctg atttatttgc taaggaagta agaaaacggc ctaagaagta
     9661 tcctaagaca gttcgattag caattgaccg ctggtatcgt tggaaaaagc gtaaagatat
     9721 ctggtttgat gttgatcgtg caaatgaaat gatggattgg gttgaatctt ttatcgttca
     9781 tacaaaaggt gacatggttg gaaaaccgtt tattctggag ccttgggaaa agtttattta
     9841 ttcatggatt tatggttggg tacgagaaaa cgaaaaaggt cagattgtcc gtgtaactcg
     9901 tgaggcatat gtccaaattc ccaaaaagaa tggtaaaaca cttattgcag tgggtgcttt
     9961 aggatatgcg atgtatggtg aaggtgcttt atctgttaac tgttatgcat gtgcttctga
    10021 ttttgctcag gcgcaatatg ctgcacaacc gtttgctgac tccattttaa ataatcctgt
    10081 tttgcttgat ggcaccaaaa tatttaaagg cccaaaaggt actgtatcaa gtattactta
    10141 tgaatactta gatggagatt tagcactgaa aaacaaattt attgttcaaa cgaaaaatat
    10201 tgaaaatatt gagggggcga atccatattt tgtctgtaat gatgagttgc acaaacagga
    10261 aaaaatggaa caatacgata attttaagtc tgcacagata tcattaccac aaccattaat
    10321 gtttaatatt tcaacagcgg gtaaaggtag cagctcggtt ggcatgcgtg tttatcgtga
    10381 agcgaaagaa gtgttaaaac gtgatgacaa tgactcatcg tttgttttaa tctatgagcc
    10441 aaataaagga tatgattgga ctgatagaaa agtttgggaa atgtgcaatc ctaactgggg
    10501 gatatcagtt gatctttccg ccttggagtc agcgtttaaa acggcacaac gttcagcaca
    10561 ttcaaaagcc gagtttctaa cgaagcactt ggatgtattt gtaaacggtg cggataattt
    10621 ctttgaacag gatcaagtag agccgtgttt ggttactacc caagagcttg gaaatttaag
    10681 tggtgaacct tgttacattg gtttggattt atcacgcaca cgagatttga cctgtgtgtc
    10741 tttaaacttc ccaacgtggg atgaagatgg aaaagcggtc cttaaagtga agcagcttta
    10801 ttttattccc aatgaagatt tagagtttcg agaaaaagaa gataacgtgc cttacagcga
    10861 tttagctgaa caaggttttg ttgaattttg cgatggtaaa atgattgatc aagatcaaat
    10921 cttgcagtac atcgaagact gcatggattt atacgatata caacaagtga attatgatcc
    10981 agcgatgagc gacaaacttg ttgagaaatt ggagaactta ggattggaat gcgttgaggt
    11041 tgcccaatat cctaaagtgt tgaacgctcc ttttgatgat gtcgaacggt tgttttatga
    11101 gaaacgaatt caatttgata atccattgtt cctttattgc accttgaacg ttgtagcaat
    11161 cactaacatc aacggacaaa aagcgccaag taaacgtcaa tcaaagaaaa agattgatgg
    11221 tttcgtggcg tttttgtgtg gtcataagga aacgatgaat cagatgacag atattgattc
    11281 ggatgaatta gatgattacc ttagttccat ctatagataa atagaaaggc ggtgagaaat
    11341 attgaaatta cgtgatcggt tatcgaatgc agtctattcc tttatggaaa agcgtgggta
    11401 catcgaggat atgtttggcc actatacgcg ttacggtcaa agatatgtaa cagattcctc
    11461 tatcatggaa tcttctgatg tttatgaatt ggtccaggat atctcaaacc aggtagcgtt
    11521 gtcctcacct gttgtcatag gtccagacgg taatgaagtg aaggaccacc acttgctaaa
    11581 aattttgaag aatcccaatg attatttgac cggattcgaa tttaccaaat tggaaacaaa
    11641 taccctactg atcaatggcg agacattccc attaacagat cgggaccagc ttcatttggc
    11701 atacggtgta accactaaaa tcaatgaacg acttcaagaa gaatttgaaa tgaacggtca
    11761 aaaaatacct ggccaaatga ttcgacacat taaaaacatc ggaactgatt cattgaaagg
    11821 tgctggaata attgatcttg caagaaacac tctggaaggc gttctgagcg ccgaaaaagt
    11881 tttgacggac aaatatacta aaggtggttt actcgcgttc atgcttaaac tggacgccca
    11941 catcaatcca aataatagcg ctcaaacgaa aattgtcaaa gcaatacttg atcagttaga
    12001 agggacgcag gatgatagca atcattcggt taaaatgatt ccacttggca aaggatacac
    12061 aattgaaacg ttaaagagtc cagttgatga tcaagctatt ttaaattatt tgggcgtgta
    12121 taaaaaagac ttagggaaat ttttaggaat caacgtagat acctaccagt ctttgatgaa
    12181 aacagacatc gaaaaagcaa tgatgtatct gcataacaaa gcaatcaaac caatattgaa
    12241 gaacaagagc gaacattaca ccgctctttt ttttatgcca aattctggct atcgggtgga
    12301 atggaaaatc aacatcttgg attttgttcc ttactcaaca aaaacaaata ttggctacaa
    12361 catcgtacgt acagggatta caagtccaga taatgtggca gaaatgcttg gttttcctaa
    12421 acagaacact ccagaaacac aagctatcta tatttcaaat gacttgtcta ggattggcca
    12481 gaaaaatgca acagatgatt ccttaccaac gaatgatcaa aacttgaaag gaggtgatgg
    12541 aaatgaagaa gaaggaaatt cgcacgattg acatcaccaa cctttcaacg cgttctgatg
    12601 aagaaactca tacgaggacc attagtggat atgctgctgt attcaatagc ccaacactat
    12661 tatgggacga tttgagtgaa gtcattgcac caggcgcttt tgctagaacg attagtaact
    12721 ccgatgtacg ttgcttattt aaccacgatt ggtccaatgt gctaggacgt actaaaagcg
    12781 ggacccttcg acttgaagaa gatgaacgtg gattgaaatt cgaagttgat ttaccagaca
    12841 caacggtagc aagggactta gttaaatcta tggaacgcgg agacatcaat caatgtagct
    12901 ttggttttgt gccgacagaa gaaacgtggg actacaattc tgaaccaatg cttcgaacaa
    12961 ttagcgaagt ggaattatat gaggtttcta ttgttccttt gccggcttat gaagatacag
    13021 aggcagcatt aagaagtcgt gatgaattag aaaaaaacgt cgaagaaaga aaaaaattaa
    13081 tcaaaaaaat taatcaagcg ctagaagcgt aggaggaaaa atacatggat acagaattat
    13141 tgaaaaaaat gaaggcacgt cgtgaacaac gattgactga attacgtgaa aaagttgaat
    13201 caggagaatt acgcgaagct gatttagaag ctattacaaa agaaattgat gccgttgttg
    13261 atgaattgaa cggaatcaaa agtgaactaa gtgaagccga tggatcagat gaaggttcag
    13321 atgctgacga aggaacaggt ggatcagacg gctcgactgg aagcgatgaa aatcgttctg
    13381 gtgaagacaa cgatcaagaa gaagattcag acagtgaaga tagttcagaa aatcgttctg
    13441 gaatgattac tcaacagcaa cgagatggat tacttggatc aattaagaac ggattggagg
    13501 cacgtgcaaa aatgaccaat aaacaaaaag atcaacaact acgaaaagca tttgctaatt
    13561 ttgtagttgg aaatatttct gaagcagaag ctcgagcttt agggattgaa gctggcaacg
    13621 gctcagttac tgtcccagaa gtaattgcat ctgaagttat tacttatgct caagaagaaa
    13681 atttacttcg taaatacgga acagtggtgc gaacatcagg agatgtcaaa tatccaattc
    13741 ttgtgaagaa agcagatgcg aatgtaaaca agaaagagcg ttcaactgat attgctgaaa
    13801 cagctattca gtttgatgaa attttgcttg atcctgccga attcgatgct ttggcaacag
    13861 taactaaaaa attactaaaa atgtctggtg ttccagttga agatattgtt gtggaagaat
    13921 tgaaaaaagc ttatgtgcgt aaagaaatca attatatgtt caatggtgat gacgctggaa
    13981 atgaaaatcc tggtgcatta gccaaaaagg ctgtagcatt tgaaaaacct ttagatctaa
    14041 ctgctgcagg tgctgggcaa aaattatatg atgcattaat cgaatttaaa aatacaccag
    14101 tgacagaagt gatgaaaaag ggacgcttta ttattaatcg agctgctttg actgctattg
    14161 aaaaaatgaa aacagatgat ggatttcctt tgttgcgtcc atttacacaa gcagaaggtg
    14221 gaataggtta ccaattagtt ggctatcctg tggattggac agatgcagca gataaaaagg
    14281 gtaaaccaga cacaccagtt ttatattttg gcgatttttc tgcatttaaa attcaagaag
    14341 ttattggtgc cttggaaatt caaaaacttg ttgaaaaatt ctctggtaaa aatcaaattg
    14401 gattccaaat ttacaacttg ctagatggtc aattggttta ttctccattt gaaccggcag
    14461 tatatcgcta cgaaattaca aaaccagttg gtggttaaga tggaagagca aactaaagaa
    14521 ttgtctttag aggaaaaatt caaatcacat attcattttg aagagggcat ggatgattct
    14581 ttgctctctt tttatttaaa tatggcaaaa gattatgtca aaacagcaac tggtggccaa
    14641 caagaatatc ttattttgat ggttgccggc attgcctatg aatatagagt ttcagaggat
    14701 gaactcgaca aagctatgaa tgccatgacg ccatttatcg tgcaaggagc gattcaaaat
    14761 gccgaagaga cagactaata atctcagatg gaaagccgaa ttgctagaca tcaaaacagg
    14821 aacagacgga aacgatcgtc caactacaat ttacgaattt aagcgtctaa tattctatga
    14881 agaactcggt gtgacttctc aagaaaaata tttatcacag caagccaaga cagacgttgt
    14941 cagacgaatt aaagtcagat gggataaatc catcacagag aaattaagtg cgctcaaaat
    15001 tgattctgta acgtataaca ttactcgcat ttatacgaat cccgatgcaa gagaaatgga
    15061 gttgagttta gcttatgtcg attagctttg atgaattgaa aacagcgctg aaatcaacaa
    15121 agttaccagt gttcagagac aaagccagat tagggacgat gtatccatac attgtgtact
    15181 caaatgtgag taacagcaaa aaaatggcat ccggtaaagt ctataaaaaa ttaccttatt
    15241 accaagtctc tttttttaca cttggaacag aacaggattt agttgttatt gaacaagcgc
    15301 tgcaaaatgc tggcattcca tattcagact ttacaggtat acaaggtgat gagaatgacg
    15361 ataccgtgac caactattac acatatgtga ggtgtgtgga aaatgccaag taataagaat
    15421 ggtttttctg aaatatcgga ttatttaggg aatctttcta gagttgatcc aaaaaaatta
    15481 tcattggaat ctttagaaga agctgcaaag ttttacctag agcagttgct tcctaatatt
    15541 cctaagtccc tgcttaaaaa gaagcatatg agcgaacaaa taaaagttgt tgttgaagaa
    15601 gaccgagtga aagtccagtt tgaagagact gctttttact ggcgctttac cgaaaatggt
    15661 acgacaaaac gaaaagcaca acactttgca agcggtacgt atgaacaaaa taaagaaaaa
    15721 atcgaagaga tcatgacgaa gaaaatactt gatttatggg aaggatgatt ttaattggga
    15781 aagcaagata ctttttattt tgaaggattg gacgacattt taatcgcgat gatggcaacg
    15841 ccagattctg ttggaactgc tccgacttat agtgaagttg tccggttgcc tatcgctaca
    15901 aaaattggag tcaaaggcaa tggtacagca ttggaaaaat gggcatcaag caaaatgttc
    15961 cgacgcgtgt cccgagaaac aaaacatgaa attgcgttgg atcatgtagg tattccaatt
    16021 gcggtaatgg atgaaatcaa agggatgatt gcaaaaagcg gagtaacatt tagtaaaaat
    16081 accgcacgag aatttcctta ccttgcattt ggattcattg gaaatattga aggcggaggt
    16141 aaaaaagctg tatggtatcc aaacacgcaa ctatctaatg taattgatga agaatacgct
    16201 actgctgaag atgaaacaaa aatcgatgat gtaactgcaa actttgtttc tacaggactg
    16261 aaatacaata atgtgatgta tgcaagtttt gattcgaatc gtgaagaagc gacaatggat
    16321 ctatttggaa aattcattgc tcaacctgta tacgatgaag aacagtggaa aacattggct
    16381 aaagttggag gtgcaggcta atggctcggt tatctgatta tggaattaac gttgaagact
    16441 taaaaaattc tgctactgtc actattcaag gtgtagaatt ccctatctca tttactatgc
    16501 aaacaatgga atttatagca gatgtgtatg gtggagatta ttcgcaattt gaatctgata
    16561 tgaacgccat gctatctaaa aaagaaggac aaatttcttc tgctaactta tcgcctagtg
    16621 atctaaaaat catgcgtgcc ttgatttatg ccatgctgcg tactggtggt ttagaggaag
    16681 atccagaaac tattttcaaa tttttgggaa tgagtggaga ggtgttgtct gcttatagta
    16741 cctgtatgga aatttttgct agccagacat ttcaggtgga agacctaaaa aaatccaaga
    16801 agccacaaga ctttcaaaaa acgcaagcaa aaagaaaggt aaacaaaaag aatcggaaga
    16861 gataggaact ccttggagtt tttatattta cgttgctctc actctattga attggagtga
    16921 gagtttcttt ttgaagtcta cacctaactt gtggctcaaa tcttatttac agtggttaca
    16981 acaaaacacc gattttgaac cacctcaatc tgtaactatg gataaatcgc cttggtggta
    17041 gaaaggagcg ctaagataaa tggctggtaa agaatctgat gtcgttctta attttaaaac
    17101 gaatggcgaa gtcagttatt cgaaaacaat caaagaaatc aacaaagaaa tgaacttagc
    17161 cgctgccgag tacaaaaacc aagtgtctgc gatggacaag gatgcaactc aaacagaaaa
    17221 attgcgagcg gctaaacaaa agttagaaaa acaactgggg ttagctgaac aacgatctca
    17281 aatgctgaga gaggaatatg aaaaatctgt caaagaaacc ggtgaatatt ctgctgaatc
    17341 agaaaaactt tataaacaat tactcaattc tgaaaccgga gaaaataaac ttcgtacagc
    17401 attagaacag acgaacgatg cacttaaaga acaaggtgac gtttctgttg atacagcaaa
    17461 aaaactccag aaaatcgaag aaacaggtga gaaagtaaaa ggcgttggtg aaaaaatgtc
    17521 tgttggagta accgcgccta ttgtagcggc aggagcagca ggacttgcag catttggtga
    17581 agttgacgag gcacttgata ccatcattac aaaaaccgga gcaacaggtg atcaagctga
    17641 tagactttca cagtctttca aaaacgttgg ttcaaatact catttacctt tacaaacggt
    17701 tggggaagct attggtgagg taaatacaca atttggattc atggataaaa aactggaaga
    17761 ttcaaccaat tatctcctac agtacgctga aatcaatgat acagatgttt cgcaatcagc
    17821 aatatctgct cgacaagcta ttgatgctta tggactagaa tatgatgatt tgaattctgt
    17881 ccttgatgta acaacgaaaa catcgcagaa tactggtcaa tctgtagacg acttgatgca
    17941 aaaagcaatt gatggcgcac cacaaattaa acaattaggg ttgagctttg gtgaaggggt
    18001 cactttactt ggacaatttg agcaaagcgg tgttgactca agtgcagctt tgagtagttt
    18061 atctaaagcg acagtagctt atgcaaaaga tggaaaatct ctaagtcagg gtctcgggga
    18121 attgcaagac aaggtaaaaa atgcaggttc tgaaacagaa gccatcaatg cagcagcaga
    18181 agtattcggg actaaaggcg gtcctagaat ggccgatgca attcgtagag gtactttgaa
    18241 cctggaagat ttagcaaaaa ctgctggaga aagtggggga gctgtaggag acacattcga
    18301 tgctactctc gacccaattg atcaagccga tcaagctatg aacaatgcaa aattagcaat
    18361 ggctgacgta ggtgaagcag tccaagtaag ccttttgcct ttttttgaaa aagcaacttc
    18421 cttattacag gaattctcta aatggtgggg gtctctagat caagatacaa agaattggat
    18481 cataacaatt gctggtatag cggcagcagt tggaccagta ctaatcgttc ttggttcact
    18541 tatgggatct gttacaaaaa tcgttggggg agttaaaagt tttataggag tttggcaagg
    18601 acttgctggt ttatttggaa tgtcgggagg ctggtttgca ttagcagtta tagctattgg
    18661 cgcattagtt actggactaa tttgggcata taataacgtt aaatggtttc atgatggagt
    18721 gaattctttc ttcaaaggtg tttcagatgt agctgttgaa atatttaatt ttttaagtgg
    18781 ttattttggt ggatttttcg atggtgttat agcaaatttc aataattttt ataatgctgg
    18841 aaaacgaatt ttcagtggtt ttctagattt tatcactgga gtatttacag ggaattggtc
    18901 aaaagcatgg caaggtctag ttgatatttt tggaggtatt ttcgatggta ttgttgctgt
    18961 agggaaagct cctattaatg ccatgattgg attgatcaat ggatttattg gtggattgaa
    19021 taatataaag ataccaaaat gggttcctgg tgtaggtgga aaatcatttt ctatttcaaa
    19081 actgccttat ttagcacaag gtggtcatct aatcaatgga caagcgattg ttggtgaagc
    19141 tggacctgag ttactgactg caaaaaatgg aaaaacaaca gttaccccat tatctgacga
    19201 agaaaaacgc agaggtattg gcggaaaagt ttctggtggt aatattgaac agcatatcca
    19261 cattggcaaa gtcgatgcaa acaatccatc tgagttagat cgtatgaatc gtaaatttgc
    19321 taaagcaaac caacaggcta tttatgattt gggaggtgtt cctatttgag tcgacaattt
    19381 atgaatccag atgaaccaaa ttttatttgg aaaaagcgga atgcagtaat tgatatggat
    19441 tgcatcattg aggcagagct tccagatatt atgccgaaca agcgatacga aacttacacg
    19501 attcagggcc gaagcggaga attgacggag acgtttgatg attatgaacc ttttgatttg
    19561 gaaatagaag ggatcactat cccacattcg aaactgaggg aagtcaaacg atggcttact
    19621 ggtaaaagcc aattaatcac tcataatgat ccagacaagt atctagatgc tatctgcagc
    19681 atggataaag aagtcccatt cgaaaacgag tggggcttct tttatacatt cgatgttact
    19741 tttcgttgtc agccactcaa aagaaaaatc ggtgaagcac ctaaaatttt tcattctagt
    19801 gttatggata tttttgatca tggtgacgaa attgcacatc cttatctgga aatagaatcg
    19861 aacggaggag atattacgat cgctgtcggt gacaaaagcc taacaatact taatacttta
    19921 gctgcaacaa tcgtggttga tactcagctt gggaaagcaa tacaagaagg tttgaattta
    19981 tttacgcgtg gtgattggcc agtattgcag cctgaatgga atcaggtaaa agtatccgga
    20041 aaatttaaag aagtgcggtt ttggaatagg agtgtgtatc tgtgacacaa gaatttattt
    20101 atgcttacaa aaaaatgcca gatgatttga gtgttaacgg agcgtcgttg gttgactggg
    20161 aagatttgcc agaaatcaat cgtgtattaa atggtcaata tcgtttttat ggtaactatt
    20221 cacgaggcgg tcagtatcgg tcttacttaa aaaaaggaaa tttcataaaa gcgaaggtac
    20281 cagatggatc atggcaatac ttcgaaattt acaatatcaa aaagaatttg acatccgttt
    20341 ctgtgacagc cagacatatt ggttttatgg ccaataaaaa ctttatcgtt aagtcattca
    20401 cggataacgg caacggttct caaatcatga ccaatcttaa aaacagtttg gcatttgatc
    20461 aaaaattcaa ctatctgtcg aatgtaggta caacgcacca atttacagca agacaagtcg
    20521 caccagttga agcgattatc ggttcaaata acggtaatca aaatttgact ggcgtgacta
    20581 gcgcggaact tgatatggat aactatgatt tgaaactggt aaaacagatt ggatcagata
    20641 atggttttcg gatcgatttt ggcatcaatt tagaagcaat cgaagaagaa attgatgaag
    20701 aatcaattgt caatagtttg tatttggttg gcggggttcc tgataacgat tatgatgaag
    20761 acaaagaacc tatcgaatat ggttatttag aaattgatgg agtgaccaat gagaatcgac
    20821 gtattgccaa acgtgaaaat ggtgattgta aaacagttga tgaattgatc aaatggggca
    20881 aaaccttatt tgacaatgac cgtattcacg agccaaaagc tactcataca gtcagcatgg
    20941 tggcgttgga acacacactc gaatacggtg agatgtatca agagctagcg tctttacact
    21001 ttggtgatgt cgctcatgtt agggcgaaag agctagacat cgagataaaa gagcgtatgg
    21061 tcgaatacac ttatttccca acgcttggga aatataagga tattgtttta ggtaatgatt
    21121 tatcactgta cacttcggca gtaaatactc aagcacaaga attgaaaaag aaaatcgaca
    21181 atcgaacaga aacgttagtt caaaatgtgt tgaatgcgac ggcatggatc actggtaatt
    21241 ctggtgggca tgtcgttttt cgtccagaaa aagctccatc agagattctt attatggata
    21301 ctcccaatgt agcgaatgcg aagcgtgtgt ggcgttggaa tttaaatggc ttaggttatt
    21361 cagacaacgg agtaaatggc ccgtttggca tcgcaatgac gtcgaagggt gaaatcgtag
    21421 ctgatttcat taaagttggg attattaatg cagaagttct tgaaagctca attaatgatc
    21481 taggtgatgt gctaaaaatt gtcaaaggta cgttacaaat ttggaataaa aataaaaaga
    21541 tcatggaatt aaccaaaaaa gggatggagt tttggaacac caaagaatcc atcggcacaa
    21601 ttggtacgac tgattctgca ggcaatcctt ttcctggcgc atccactcct acaccgttag
    21661 aagataattc gttagtcatt cgaacaaatg gcgatggaaa atatattctc atttcaccaa
    21721 aagaaggaaa aggttgggta atacttggga atggaacttc tatattattt ggaagtttaa
    21781 acctacagga aaagctaaat gcctttggtg atgcggaatt tatgaaaaac gtcaatattc
    21841 gcggaaaact cacaattaac ggacaagaag tattccctgg acaaggcgga agtggaaata
    21901 atgatggcgg tagttggaat ggcatgtacc caccagaggt taccagtcaa gcagacaaat
    21961 ttgcatggga attatgggtc atgcttcttt ctagagggta ttccaaagca tccatcgctg
    22021 gaatccttgg aaatgttcaa ggagaagccg gtgctgcaat gaatccagat attgcacaag
    22081 tcggcggtcc agcttatgga atcgttcaat gggatggttc ggcatatcct ttggttcctc
    22141 cagcaacatg ggatggcagg acttatgttc aaaacctgat gagaaccgca ggaattacag
    22201 aagattatcg aacaatgtca gctcaaggga agttattaga ttggacgatg tataacggtc
    22261 aatggttagg aattgttcaa ccagcaagtg tttctggatt taaagcaatg actgatccag
    22321 cagcggctgc ttatactttt gaacgaaatt atgaaagacc agccacaact catccagaaa
    22381 gacaaggatg ggcagttaat tggtataaca aattcaaaga tcttcaagtc ccatctgctg
    22441 gcagtattct cagtacagcc aaaagcttga tgggttattt ccattactcg caaccgttac
    22501 gttggaattt tggtagcgtc gagaatcctg atcgtaatgg gtatgctgac tgttcttctt
    22561 ttgtttggtt agctttgaca aaatcgggat ataaaaccgc aacacgtgga actctgtggt
    22621 atacaggctc aatggctgcg gatgcaagag ggccacgtca gtatctgact gaaatatctc
    22681 caaataaagc gaaagccgga gatatcatta ttgtcaacca aggagccggt gctggtaatg
    22741 atggacacac tgctatttta gcagaggatt ggaagggata tagcacgtct atcgttgaaa
    22801 tgggcggtat gaattccaat ggtgtaggta tcggtcgcgt cgattggtcc ttcggatatt
    22861 tattaaacgg tggcgatgtt tgtctcgcca gagcgaagaa atagaggtga ttttgtgatc
    22921 gaagaaaaag gattaaatca tttgaaaagt ttgttgaatc aacctatcgg aaatcatcaa
    22981 tgttatgcat tatctgcgga atatgccggt gtgatgattg gacctgacat gggggctggt
    23041 actaaatatg agattaaagt acgtcatggc aatgtatttt ccgctgctga aattggacga
    23101 gcctatccat gggcattgta tttatggacg gttattgttc atcccgagta tgaccaacta
    23161 gttgttggct caattatcaa ttgggaaaga aacgcaaaaa tcagtgatac atttgaaagc
    23221 catgaatatt acggccacac aggtgtaatc aaaggtctgg aaaatgggcg tattcaaacc
    23281 tatgaacaaa atgcagaatc aggtggaatt gtggcggaat atgaccgtga attcttcgga
    23341 tctggtcaga tagcctctat ttgtatcccg cctgattttg agaaaggagt ggtaattaat
    23401 ggcaaagtgg aacgtagtac tcagcacaac tgaaccttat aactatgtcg gtatgattca
    23461 ggtacggcaa ggcaataaga attccgaagt tatggaagca accatcactg aaaatggaat
    23521 gccctatgat ctaactggtt gtaaagtcta ttttgaatca gttgtcggag acaaataccc
    23581 agttcaatta ggtactaaag tcattgatgc aaaaaaagga aaaattcagt acacatttga
    23641 tcaatattcg atgcaatgcc tacatcgcca aacagcagat tttatcattt ataaagatga
    23701 tgagttaatt gctacgacgc aagacttctc ctattttgtg attaaagccg tctcaaaaac
    23761 agagggtgaa atgggatcgt attggcagac agtcgaagat ttaattgcgg atatgtcagc
    23821 ttttatcaat gaaaacaaag gtgacttcac agattggatg aatgcacgta aaaaggaatt
    23881 tgaacaatgg cgacaagacc aacaaaatac atttgaggca tggagagaag gacaagaaac
    23941 cgattattta aaatggtttg aatcaatcaa
//
LOCUS       DAFBYI010000036        23875 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFBYI010000036 DAFBYI010000000
VERSION     DAFBYI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.036"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(211..516)
                     /locus_tag="LLD04_001216"
     CDS             complement(211..516)
                     /locus_tag="LLD04_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK5648159.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(541..972)
                     /gene="ruvX"
                     /locus_tag="LLD04_001217"
     CDS             complement(541..972)
                     /gene="ruvX"
                     /locus_tag="LLD04_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK5648160.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(969..1235)
                     /locus_tag="LLD04_001218"
     CDS             complement(969..1235)
                     /locus_tag="LLD04_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK5648161.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1490..2215)
                     /locus_tag="LLD04_001219"
     CDS             complement(1490..2215)
                     /locus_tag="LLD04_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK5648162.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2576..2646)
                     /locus_tag="LLD04_001220"
     tRNA            complement(2576..2646)
                     /locus_tag="LLD04_001220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2612..2614),aa:Gly,seq:ccc)
     gene            2763..3515
                     /locus_tag="LLD04_001221"
     CDS             2763..3515
                     /locus_tag="LLD04_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5648163.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3531..4010
                     /locus_tag="LLD04_001222"
     CDS             3531..4010
                     /locus_tag="LLD04_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5648164.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4261..5841)
                     /locus_tag="LLD04_001223"
     CDS             complement(4261..5841)
                     /locus_tag="LLD04_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK5648165.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5834..6577)
                     /locus_tag="LLD04_001224"
     CDS             complement(5834..6577)
                     /locus_tag="LLD04_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648166.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6753..7529)
                     /locus_tag="LLD04_001225"
     CDS             complement(6753..7529)
                     /locus_tag="LLD04_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648167.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7777..8613)
                     /locus_tag="LLD04_001226"
     CDS             complement(7777..8613)
                     /locus_tag="LLD04_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK5648168.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8624..9553)
                     /locus_tag="LLD04_001227"
     CDS             complement(8624..9553)
                     /locus_tag="LLD04_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK5648169.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9745..10098
                     /locus_tag="LLD04_001228"
     CDS             9745..10098
                     /locus_tag="LLD04_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5648170.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10172..10828)
                     /locus_tag="LLD04_001229"
     CDS             complement(10172..10828)
                     /locus_tag="LLD04_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5648171.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11054..12988
                     /locus_tag="LLD04_001230"
     CDS             11054..12988
                     /locus_tag="LLD04_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5648172.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13074..14297)
                     /locus_tag="LLD04_001231"
     CDS             complement(13074..14297)
                     /locus_tag="LLD04_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5648173.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14291..16189)
                     /locus_tag="LLD04_001232"
     CDS             complement(14291..16189)
                     /locus_tag="LLD04_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK5648174.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16183..17301)
                     /locus_tag="LLD04_001233"
     CDS             complement(16183..17301)
                     /locus_tag="LLD04_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5648175.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17384..18217)
                     /locus_tag="LLD04_001234"
     CDS             complement(17384..18217)
                     /locus_tag="LLD04_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5648176.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18210..19127)
                     /locus_tag="LLD04_001235"
     CDS             complement(18210..19127)
                     /locus_tag="LLD04_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5648177.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19354..20478)
                     /locus_tag="LLD04_001236"
     CDS             complement(19354..20478)
                     /locus_tag="LLD04_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5648178.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20661..21569
                     /locus_tag="LLD04_001237"
     CDS             20661..21569
                     /locus_tag="LLD04_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648179.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21710..23324
                     /locus_tag="LLD04_001238"
                     /pseudo
     CDS             21710..23324
                     /locus_tag="LLD04_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         7438 a         4658 c         3996 g         7783 t
ORIGIN      
        1 gagaacaccg tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa
       61 caaatggcaa aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct
      121 gattgaacat cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt
      181 ttttatatct tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgtatt
      241 gaatacttct tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc
      301 tgttccatct tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga
      361 aacaccagca ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt
      421 tagtaggatt tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg
      481 tccttcatgg tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta
      541 ttaaaataat tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa
      601 tttatcgatg actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg
      661 ttctgcctga acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc
      721 tttgatcttt tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat
      781 atttttaggt aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa
      841 ccgatcaaag ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc
      901 tgtccagcca aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc
      961 cattactctc ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg
     1021 atctcatcgc gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag
     1081 gccgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct
     1141 tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca
     1201 aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg
     1261 taattccagc ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa
     1321 atattccaaa cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct
     1381 gtagctttat caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt
     1441 ttttacacaa ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg
     1501 tttttgttgc ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa
     1561 agacattgaa ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg
     1621 ctaccagttc attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt
     1681 ctttgatata tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac
     1741 gtgtccctgt catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta
     1801 aatggctgcg attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg
     1861 ggtcaatata caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat
     1921 tggcttgtcc tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg
     1981 aaaggctaag atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat
     2041 gttttccatc atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa
     2101 tagttgtaat ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc
     2161 ctgcaatcac agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt
     2221 tactccttta ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag
     2281 aaagaaatag aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa
     2341 gagacggatt cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt
     2401 caaaaatttt cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg
     2461 cgcaaaagct tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt
     2521 ttgcttggct gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg
     2581 gtgacgggaa tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac
     2641 acccgcaata caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt
     2701 tacgcttctg gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg
     2761 ctatgacggg aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca
     2821 tgatggaaag caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag
     2881 cttctggaaa tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg
     2941 atcgtacctc gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag
     3001 tagtaaaagt aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac
     3061 tttactgcga actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa
     3121 tcgttagcgc ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg
     3181 atttacctgt gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga
     3241 aattttttag aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg
     3301 ctatctacag cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg
     3361 tgatgccttt agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac
     3421 attatgtctc aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt
     3481 ttgcgattgc ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga
     3541 tatttattca gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg
     3601 tacctaggcg ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact
     3661 ggatctgtgt tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag
     3721 tagcattcat cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata
     3781 ttcccgggat ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg
     3841 ttactgaatc ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag
     3901 caatcgcttt aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag
     3961 taaagcgtga cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta
     4021 acaaagaaaa ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa
     4081 cattgcgaca agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt
     4141 gtaaaaatag cttctcacat ttttacataa aattgactta atactgagaa actgactttt
     4201 gaacaccctc tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct
     4261 ttattcaaat tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat
     4321 tagaactaaa ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat
     4381 tccgtataag acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa
     4441 acccaaactt cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag
     4501 caaccgataa tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc
     4561 aaatagcaag ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc
     4621 gatcccacca gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata
     4681 ttggttcata gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat
     4741 aagattgctg ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag
     4801 gaaaacaaca ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc
     4861 aaaagcaaga ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa
     4921 attaggttct tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt
     4981 ctttttatgg gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag
     5041 ctttggtact atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat
     5101 tccccaaccc aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa
     5161 taatatcgaa atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt
     5221 catccacatg attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac
     5281 tgtttttcta tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc
     5341 cagacaaaaa agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag
     5401 caaaatccct agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac
     5461 aaaaggaatg atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc
     5521 aaaaggcaga ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac
     5581 tgatatactt tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc
     5641 tggcaattta acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag
     5701 ccctgaaaaa agattttgag acaaaagata gatagttaat cgctttccct tttttccttt
     5761 ctctcttgct ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc
     5821 taataattgt cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca
     5881 aataaatctt ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct
     5941 gataaatcaa ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat
     6001 ccaatacatg tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt
     6061 ctttcaaatc aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa
     6121 tatctggatc tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa
     6181 aacttgcgat tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta
     6241 aacgcttttc ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt
     6301 ctccagccgt caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt
     6361 tgatactcaa tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg
     6421 gttcaataat actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta
     6481 aaaatccaaa aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt
     6541 tcccgtaact tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta
     6601 ttacttttta ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa
     6661 aaaaggatag aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct
     6721 tctaacaggt aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct
     6781 tgcataaaat tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt
     6841 gcaaatgtta ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata
     6901 taagctttta aatctttaca taaatactca tattcgagtg attttttata ttttttaatt
     6961 gtgccgttta cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct
     7021 ggcatgctca ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa
     7081 cggacagttg tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc
     7141 aataagtaac tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa
     7201 ccactaggcg taatctttcg attaataaca gttattgctt tattcggatg agacgttaga
     7261 taaacaattt tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta
     7321 taataggcgt tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt
     7381 ttaatgaatt caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga
     7441 cttaataaaa ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta
     7501 tcatccaaaa taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc
     7561 aaaatcattt catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac
     7621 aatcttttcc aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat
     7681 aaatatcaaa caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg
     7741 tcagctgtct gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg
     7801 atttttcgga gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt
     7861 gaatggttga taatagtctg attggatggt tccttctatt ttataccatt catcattttt
     7921 aaccgttact ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca
     7981 atggataatg ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta
     8041 aacaaatcct tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag
     8101 ctccataact tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt
     8161 ttctttcaac tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc
     8221 cggctttaag tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa
     8281 attaaatcct ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc
     8341 cacgattact ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa
     8401 cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa
     8461 atacagctga actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg
     8521 ataatggaca ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc
     8581 gaaataccca gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga
     8641 atccaataac gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat
     8701 atcgtttcat catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag
     8761 ctacaggtgc tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg
     8821 agcaaagaga catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg
     8881 agccgagcgt tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc
     8941 cgccaatgat caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat
     9001 gcccagtttg atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat
     9061 ggacatgatg atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt
     9121 ctttttgaaa ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca
     9181 cccgccagct tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga
     9241 ttggattgat gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt
     9301 ttttaacaaa ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc
     9361 ctaagatcga accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac
     9421 gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag
     9481 gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa
     9541 taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg
     9601 cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca
     9661 aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact
     9721 tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac
     9781 atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt
     9841 cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat
     9901 ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt
     9961 gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa
    10021 aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa
    10081 atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt
    10141 ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat
    10201 ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt
    10261 ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga
    10321 ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa
    10381 ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg
    10441 ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac
    10501 gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac
    10561 cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa
    10621 gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc
    10681 gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt
    10741 tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg
    10801 tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc
    10861 tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt
    10921 gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca gtataatgga
    10981 atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag
    11041 aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga
    11101 agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg
    11161 cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga
    11221 tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg
    11281 acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca
    11341 aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc
    11401 tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa
    11461 cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc
    11521 attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc
    11581 tcgcatgctt ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga
    11641 tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat
    11701 tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag
    11761 aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca
    11821 gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga
    11881 cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa
    11941 gacattagaa aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt
    12001 tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat
    12061 cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc
    12121 gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca
    12181 ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta
    12241 cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga
    12301 gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg
    12361 cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt
    12421 agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt
    12481 agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct
    12541 ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc
    12601 tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga
    12661 agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac
    12721 gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg
    12781 aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga
    12841 acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca
    12901 agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag
    12961 cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg
    13021 ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa
    13081 taagaagacc aattactagt cattctcact aaagcttcta aataatagta atcaccccaa
    13141 atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag
    13201 ccatcttctt cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca
    13261 gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat
    13321 aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca
    13381 aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct
    13441 aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa
    13501 ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc
    13561 gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa
    13621 ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta
    13681 tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa
    13741 tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact
    13801 cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc
    13861 atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca
    13921 agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct
    13981 ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt
    14041 cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata
    14101 ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg
    14161 tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat
    14221 tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc
    14281 ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc
    14341 cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc
    14401 gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct
    14461 ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa
    14521 tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag
    14581 cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag
    14641 aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt
    14701 gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag
    14761 taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc
    14821 gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg
    14881 tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg
    14941 ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg
    15001 tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat
    15061 catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag
    15121 gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct
    15181 tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt
    15241 aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc
    15301 ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc
    15361 acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag
    15421 gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc
    15481 ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat
    15541 aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc
    15601 agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt
    15661 taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat
    15721 taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt
    15781 ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct aataacattg
    15841 cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg
    15901 aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt
    15961 ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt
    16021 tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa
    16081 acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta
    16141 atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc
    16201 cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac
    16261 attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc
    16321 catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg
    16381 atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc
    16441 gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat
    16501 ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag
    16561 atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt
    16621 tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt
    16681 ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata
    16741 cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc
    16801 cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg
    16861 tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac
    16921 cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc
    16981 aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag
    17041 ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt
    17101 cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc
    17161 tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc
    17221 cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca
    17281 ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag
    17341 ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc
    17401 caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca
    17461 ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa
    17521 ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta
    17581 atggaaatag atattgattc caacttccca taaagataag taaaccacta gtaataatcg
    17641 taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga
    17701 ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca
    17761 ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta
    17821 aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata
    17881 gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac
    17941 ccgccattgt agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat
    18001 tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca
    18061 tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag
    18121 ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt
    18181 acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat
    18241 tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt
    18301 ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa
    18361 ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca
    18421 ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat
    18481 aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta
    18541 gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc
    18601 atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta
    18661 attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat
    18721 acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat
    18781 ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc
    18841 gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat
    18901 ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt
    18961 ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa
    19021 ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt
    19081 ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc
    19141 taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc
    19201 ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa
    19261 ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat
    19321 tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc
    19381 ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa
    19441 gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc
    19501 atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt
    19561 tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga
    19621 catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc
    19681 agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt
    19741 tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga
    19801 ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc
    19861 aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca
    19921 agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca
    19981 aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg
    20041 caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc
    20101 atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc
    20161 catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac
    20221 tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg
    20281 gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac
    20341 ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt
    20401 agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa
    20461 tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt
    20521 tcacttatgt atattaacgg aattagtaac acaaatattt atataaaaaa atattttact
    20581 tgtcgttttg gcttttcatt tttgttataa tagtcattaa ttataaatag aaagcttttt
    20641 atataaaaag agaaaggaaa gtggttcttt tgttaacaaa caaccaatcc tccaatcttg
    20701 tcaaaaaaac actaacctat tccatagata cccatcttca tttatctgct gctccctatt
    20761 ttcgactact aataaatgaa agcgctaatt tatatgctga atttgcacat aaatctatag
    20821 aactagataa tccttccctt ttattcattg ataaacagca agaagtccaa atcttttcta
    20881 ctcaaaagga aacacaaact tttatcatta tccagtttta ttttgatgag caattattag
    20941 cacatattac gggacaatct ttttctatag atcaagaaat aacatctatc attacttttc
    21001 tgaaaaatga acagttcaaa attgattatc taaaacatag taagccagct atttcctatg
    21061 atcaggaaat caattctttt ttcatactat gccaacagat gagcattcat ttgctttcct
    21121 tattagttac ttattgtctg caaaaaattc aattgtcttc cttaccaagg cattatcaac
    21181 aggaacttgc tttattgaca ataccttcaa atgagaaagt cattcctaaa aaaaagcata
    21241 ttctttattc agatgaatta gttgatccaa tcatacgata tttagaagaa catctttcag
    21301 aaaatttttt gcttgaacaa ttagcccaaa ttttttttat cagtccagcc agcttaaaaa
    21361 aacattttag aaaagtgaat ggaaaaagtg ttattagcta ttttaaagaa ctaaaaatgg
    21421 aaaaagcaaa acgtctcgtg ctagaaaatg aattaaccta tacagaaatt gcagatatat
    21481 taggatttca ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc
    21541 cttcaaagta ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta
    21601 tttctctata ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct
    21661 gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt
    21721 tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga
    21781 agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt
    21841 gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga
    21901 aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga
    21961 tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga
    22021 ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt
    22081 ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc
    22141 gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct
    22201 ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc
    22261 acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa
    22321 agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga
    22381 aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt
    22441 ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat
    22501 cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat
    22561 cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa
    22621 tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata
    22681 tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga
    22741 acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga
    22801 ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga
    22861 tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg
    22921 agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt
    22981 gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc
    23041 aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga
    23101 tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat
    23161 ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa
    23221 agataaaaaa agtttacatt actttcagaa gaggattgaa tacttatact tctgcccgaa
    23281 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
    23341 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
    23401 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
    23461 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
    23521 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
    23581 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
    23641 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
    23701 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
    23761 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
    23821 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta tttaa
//
LOCUS       DAFBYI010000037        23650 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFBYI010000037 DAFBYI010000000
VERSION     DAFBYI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.037"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            248..523
                     /locus_tag="LLD04_001239"
     CDS             248..523
                     /locus_tag="LLD04_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648180.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="LLD04_001240"
     CDS             536..2614
                     /locus_tag="LLD04_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK5648181.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESCTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="LLD04_001241"
     CDS             2774..4660
                     /locus_tag="LLD04_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5648182.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="LLD04_001242"
     CDS             4672..5619
                     /locus_tag="LLD04_001242"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK5648183.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="LLD04_001243"
     CDS             5639..6166
                     /locus_tag="LLD04_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5648184.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="LLD04_001244"
     CDS             complement(6229..6882)
                     /locus_tag="LLD04_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK5648185.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="LLD04_001245"
     CDS             7014..7856
                     /locus_tag="LLD04_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5648186.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8910
                     /locus_tag="LLD04_001246"
     CDS             8014..8910
                     /locus_tag="LLD04_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5648187.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8913..9626
                     /locus_tag="LLD04_001247"
     CDS             8913..9626
                     /locus_tag="LLD04_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK5648188.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9646..10164
                     /gene="msrA"
                     /locus_tag="LLD04_001248"
     CDS             9646..10164
                     /gene="msrA"
                     /locus_tag="LLD04_001248"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5648189.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10161..10388
                     /locus_tag="LLD04_001249"
     CDS             10161..10388
                     /locus_tag="LLD04_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK5648190.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10540..11091
                     /gene="lepB"
                     /locus_tag="LLD04_001250"
     CDS             10540..11091
                     /gene="lepB"
                     /locus_tag="LLD04_001250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5648191.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11174..11674)
                     /locus_tag="LLD04_001251"
     CDS             complement(11174..11674)
                     /locus_tag="LLD04_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5648192.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11953..12351)
                     /locus_tag="LLD04_001252"
     CDS             complement(11953..12351)
                     /locus_tag="LLD04_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5648193.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12611..13471
                     /gene="ylqF"
                     /locus_tag="LLD04_001253"
     CDS             12611..13471
                     /gene="ylqF"
                     /locus_tag="LLD04_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK5648194.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13464..14231
                     /locus_tag="LLD04_001254"
     CDS             13464..14231
                     /locus_tag="LLD04_001254"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK5648195.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14287..15144
                     /gene="dprA"
                     /locus_tag="LLD04_001255"
     CDS             14287..15144
                     /gene="dprA"
                     /locus_tag="LLD04_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK5648196.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15266..17344
                     /gene="topA"
                     /locus_tag="LLD04_001256"
     CDS             15266..17344
                     /gene="topA"
                     /locus_tag="LLD04_001256"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK5648197.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKSAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17313..18689
                     /gene="trmFO"
                     /locus_tag="LLD04_001257"
     CDS             17313..18689
                     /gene="trmFO"
                     /locus_tag="LLD04_001257"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK5648198.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18887..19792
                     /gene="xerC"
                     /locus_tag="LLD04_001258"
     CDS             18887..19792
                     /gene="xerC"
                     /locus_tag="LLD04_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK5648199.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19829..20377
                     /gene="hslV"
                     /locus_tag="LLD04_001259"
     CDS             19829..20377
                     /gene="hslV"
                     /locus_tag="LLD04_001259"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK5648200.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20391..21791
                     /gene="hslU"
                     /locus_tag="LLD04_001260"
     CDS             20391..21791
                     /gene="hslU"
                     /locus_tag="LLD04_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK5648201.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21809..22600
                     /gene="codY"
                     /locus_tag="LLD04_001261"
     CDS             21809..22600
                     /gene="codY"
                     /locus_tag="LLD04_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK5648202.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22682..23557
                     /locus_tag="LLD04_001262"
     CDS             22682..23557
                     /locus_tag="LLD04_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK5648203.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8191 a         4080 c         4981 g         6398 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gacgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tgtacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa
     8701 ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca
     8761 gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat
     8821 aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac
     8881 caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa
     8941 acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac
     9001 ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag
     9061 aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaag
     9121 aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat agtgatattg
     9181 aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa gtactaggtt
     9241 ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga aatgtccaac
     9301 taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat gtgatgaata
     9361 tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat gatctgacta
     9421 tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa gcagataaaa
     9481 tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat acctctgatg
     9541 cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc aagacaaaag
     9601 aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga acgagcaata
     9661 tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc tggtatccat
     9721 acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga acaagtaaaa
     9781 agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac attgatttca
     9841 tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc tttcggacaa
     9901 ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga agaacagcgg
     9961 atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga acagccaatc
    10021 gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca tcaagagtat
    10081 tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa
    10141 gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg acactgcgag
    10201 gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa gacatccaat
    10261 ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg aatgtcgact
    10321 atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa aataatcatg
    10381 acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca atagcctaat
    10441 tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt ttcttataat
    10501 aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa acaacgctat
    10561 attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct tgcgttcatg
    10621 ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga aaacgtgttg
    10681 aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt tgatattgtt
    10741 gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct tccaggagaa
    10801 aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa ggaaccttat
    10861 ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga ctttacacta
    10921 caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact tggagataat
    10981 cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga tattctaggt
    11041 aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg agaaacaata
    11101 aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca gtttactacc
    11161 ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa gttttgataa
    11221 atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg aatctaccta
    11281 gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa ttgataaaaa
    11341 ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg tttatctttt
    11401 tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata agaaaagcag
    11461 cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac atttcaagca
    11521 aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa aatccgaatg
    11581 tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca agggctaata
    11641 aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa acgtcttttg
    11701 caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg ataccataaa
    11761 atcaccaatg tgtcacgaca cattggtgat ttacatacac tggcatctaa caattaaaaa
    11821 aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata
    11881 gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa aaacgtcaga
    11941 ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt ctagatcttg
    12001 gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt ggaaagtacc
    12061 accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt caacactaaa
    12121 cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt ttataaattt
    12181 tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata ccattgaacc
    12241 agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat aaaaagcaac
    12301 ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca tttgtttcgt
    12361 ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca aaaatttaga
    12421 ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa
    12481 tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat aaaaaagtga
    12541 atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat
    12601 ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt
    12661 ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt
    12721 atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt
    12781 gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa
    12841 acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt
    12901 acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa
    12961 gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat
    13021 gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg
    13081 tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg
    13141 gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga
    13201 ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg cccgatatta
    13261 ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc
    13321 ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag
    13381 cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg
    13441 cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga
    13501 aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag
    13561 gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg
    13621 aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga
    13681 ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag
    13741 ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga
    13801 aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga
    13861 tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga
    13921 tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct
    13981 tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg
    14041 ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc
    14101 tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag
    14161 gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat
    14221 atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt
    14281 tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt
    14341 agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga
    14401 tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt
    14461 tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc
    14521 tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt
    14581 tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac
    14641 acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga
    14701 tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc
    14761 tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct
    14821 gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct
    14881 cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg
    14941 tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag
    15001 caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat
    15061 tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat
    15121 atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt
    15181 ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg
    15241 acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc
    15301 tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt
    15361 tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga
    15421 accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga aatccgcagc
    15481 aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc
    15541 ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa
    15601 tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga
    15661 tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag
    15721 tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc
    15781 tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg
    15841 gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat
    15901 cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat
    15961 tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc
    16021 gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac
    16081 aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg
    16141 aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa
    16201 agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatgatca
    16261 ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag
    16321 tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta
    16381 tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat
    16441 gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt
    16501 caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa
    16561 tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca
    16621 acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga
    16681 aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta
    16741 ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc
    16801 attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg
    16861 agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta
    16921 taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa
    16981 agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg
    17041 gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat
    17101 cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa
    17161 atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc
    17221 ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa
    17281 agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa
    17341 ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg
    17401 gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc
    17461 gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca
    17521 attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc
    17581 gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct
    17641 tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat
    17701 tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa
    17761 caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg
    17821 gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata
    17881 aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga
    17941 cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta
    18001 aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc
    18061 gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa
    18121 ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat
    18181 acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga
    18241 tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct
    18301 ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt
    18361 tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac
    18421 ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag
    18481 aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac
    18541 cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag
    18601 caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat
    18661 tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag
    18721 tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg
    18781 ccaattgtga acaaacttag aaatattggt taaattctga caatttattt gtctttttat
    18841 aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac
    18901 gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa
    18961 agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc
    19021 tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca
    19081 tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta
    19141 tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa
    19201 aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga
    19261 cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta
    19321 cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga
    19381 ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc
    19441 ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa
    19501 atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac
    19561 tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca
    19621 tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg
    19681 gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt
    19741 aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg
    19801 aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat
    19861 ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg
    19921 tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt
    19981 tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg
    20041 caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg
    20101 gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga
    20161 aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat
    20221 cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa
    20281 cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat
    20341 ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat
    20401 taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg
    20461 cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg
    20521 aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg
    20581 gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg
    20641 aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg
    20701 atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa
    20761 gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag
    20821 aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc
    20881 aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag
    20941 tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag
    21001 accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa
    21061 atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag
    21121 aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc
    21181 atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata
    21241 agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca
    21301 tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc
    21361 acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag
    21421 aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg
    21481 tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta
    21541 cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt
    21601 tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa
    21661 aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca
    21721 cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct
    21781 atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat
    21841 aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa
    21901 aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg
    21961 agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt
    22021 tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga
    22081 agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata
    22141 tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat
    22201 tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg
    22261 agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt
    22321 acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc
    22381 tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat
    22441 tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc
    22501 agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa
    22561 ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag
    22621 atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc
    22681 aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga
    22741 attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc
    22801 gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca
    22861 gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga
    22921 ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga
    22981 gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga
    23041 cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat
    23101 cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta
    23161 tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga
    23221 tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt
    23281 tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga
    23341 aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc
    23401 tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac
    23461 ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga
    23521 aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc
    23581 agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg
    23641 acatcgattt
//
LOCUS       DAFBYI010000038        23603 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFBYI010000038 DAFBYI010000000
VERSION     DAFBYI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.038"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..726
                     /locus_tag="LLD04_001263"
     CDS             <1..726
                     /locus_tag="LLD04_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5648204.1"
                     /translation="IQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1045..2364)
                     /locus_tag="LLD04_001264"
     CDS             complement(1045..2364)
                     /locus_tag="LLD04_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBK5648205.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2488..3177
                     /locus_tag="LLD04_001265"
     CDS             2488..3177
                     /locus_tag="LLD04_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBK5648206.1"
                     /translation="MVLIKSMNYSGFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDL
                     NHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNE
                     IVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDG
                     QYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQ
                     KIKEKLKESTV"
     gene            complement(3880..4095)
                     /locus_tag="LLD04_001266"
     CDS             complement(3880..4095)
                     /locus_tag="LLD04_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBK5648207.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(4114..4350)
                     /locus_tag="LLD04_001267"
     CDS             complement(4114..4350)
                     /locus_tag="LLD04_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBK5648208.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            4608..6761
                     /locus_tag="LLD04_001268"
     CDS             4608..6761
                     /locus_tag="LLD04_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBK5648209.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6730..8145
                     /locus_tag="LLD04_001269"
     CDS             6730..8145
                     /locus_tag="LLD04_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK5648210.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            8178..9212
                     /gene="sppA"
                     /locus_tag="LLD04_001270"
     CDS             8178..9212
                     /gene="sppA"
                     /locus_tag="LLD04_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK5648211.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            9214..9735
                     /locus_tag="LLD04_001271"
     CDS             9214..9735
                     /locus_tag="LLD04_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5648212.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            9732..10118
                     /locus_tag="LLD04_001272"
     CDS             9732..10118
                     /locus_tag="LLD04_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK5648213.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(10614..10686)
                     /locus_tag="LLD04_001273"
     tRNA            complement(10614..10686)
                     /locus_tag="LLD04_001273"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10651..10653),aa:Lys,seq:ctt)
     gene            11067..11636
                     /locus_tag="LLD04_001274"
     CDS             11067..11636
                     /locus_tag="LLD04_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK5648214.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            11623..12483
                     /locus_tag="LLD04_001275"
     CDS             11623..12483
                     /locus_tag="LLD04_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5648215.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            12673..13377
                     /locus_tag="LLD04_001276"
     CDS             12673..13377
                     /locus_tag="LLD04_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648216.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            13382..15217
                     /gene="walK"
                     /locus_tag="LLD04_001277"
     CDS             13382..15217
                     /gene="walK"
                     /locus_tag="LLD04_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK5648217.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            15214..16530
                     /locus_tag="LLD04_001278"
     CDS             15214..16530
                     /locus_tag="LLD04_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648218.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            16531..17400
                     /locus_tag="LLD04_001279"
     CDS             16531..17400
                     /locus_tag="LLD04_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK5648219.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            17455..18264
                     /locus_tag="LLD04_001280"
     CDS             17455..18264
                     /locus_tag="LLD04_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5648220.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(18367..18996)
                     /locus_tag="LLD04_001281"
     CDS             complement(18367..18996)
                     /locus_tag="LLD04_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK5648221.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            19166..20458
                     /locus_tag="LLD04_001282"
     CDS             19166..20458
                     /locus_tag="LLD04_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5648222.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(20534..21196)
                     /locus_tag="LLD04_001283"
     CDS             complement(20534..21196)
                     /locus_tag="LLD04_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5648223.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            21421..21705
                     /gene="groES"
                     /locus_tag="LLD04_001284"
     CDS             21421..21705
                     /gene="groES"
                     /locus_tag="LLD04_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK5648224.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            21756..23381
                     /gene="groL"
                     /locus_tag="LLD04_001285"
     CDS             21756..23381
                     /gene="groL"
                     /locus_tag="LLD04_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK5648225.1"
                     /translation="MAKELKFAEDARAAMLHGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         8020 a         3827 c         4722 g         7034 t
ORIGIN      
        1 attcaaatgt ttgttgttct atttttattt attagaataa ctattaggca acgagaacaa
       61 tatgaacagc aattgaaaag aaaagaattg gtttatctaa aaaaatatac tgattcactg
      121 gaaaaagatc aagaaaaatt tgccagattt agacatgact ataaaaattt attgctgagt
      181 ttaaaagaaa tcgctaatca aaaccatgat tcgatattgg aaaaagaaat aaaagaatta
      241 gagatctatt ctaattctta tctaaccaat aagtttgaat atcgttattt aaaaaatata
      301 aaaaatgaat atttgaaaag tttgttgata gcgaagctat accaagcaaa taacaataag
      361 atttgttgta aattcgaatg tccaagtact attattgaag ttcctgttcc aatatttgac
      421 tgtattaggg tgcttggaat tattctcgat aatgcaattg aagcatctat agagagtcaa
      481 gatagagaac tgtctctagc tatttaccga gatgataaac agatagaaat ttcgatatcg
      541 aatagttgta gagagatact actctctata gatactctaa tgaaaaaagg aataacaaca
      601 aaaaagggac atcaagggtt aggtttatct agtatagagg aaatcaataa aaagaatccc
      661 aatatgtttg tgaactatca aaacgatccg tatcaattta ctactcaagt tattttgttg
      721 ttttgaggga aggaggattt atgagttatc caataatatt atgtgaagat caaattatac
      781 aattaaatca gttagaacga attattgata atttcattct ttttcacgat aaagtattta
      841 acctgaataa ttcatagctt taattctttg gcacatttta ttgaaatatg tatcactaat
      901 atccctatca actgattttg actaaaaagt tacttcaaac attcactgtt caaaaatatt
      961 gaggaataaa ttttgggtgt aaggcatttt gagcatactt ctccctggat aacttcccag
     1021 taaaaaaatc gttagattgt tggattagat ccattgactg gctcgcctca ggcgagcaaa
     1081 gaccctgtag aattcattct gatacacatg ataactagat aatttgagat agactcgtct
     1141 acccgtttgg actaatttcc cagcaacttt cagaaacttc aatcgaatag aatgaatggt
     1201 cattcccttt tggacttctt caaagccaat cgttcttaag aagttgacta agttgtaagc
     1261 tatcaagctg agcatcattc ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc
     1321 aaagtaaaag ccagctttcg cttctttaat gaagttctcc attgtgccac gtttggcata
     1381 gatagaaaag atggtatcag gagaaacatt ttctgataga ttcgtcacga taaattcatg
     1441 tcgaaagagt agttcgcccg cttcacgtgt tgagcgtata cacacgcgac gactttgtga
     1501 ccaggattgt gcttgataag tggtcgagaa gtactgaact tctcgttctt cccacttttg
     1561 attatcgcca taaagtactg atttctcagc tatttgacct agtctacgat tattcttcag
     1621 tcgaataaca taatgactct tttttgattc acacgaatca tacacaccag gtgtagcgaa
     1681 cccgctgtct ccacgaacca agatgtcagt gtttggtaga gagtgattat agtgctctaa
     1741 taaaggtgtg agaaactcct ttacgccttt tgatgtatat tgatttcctg aacgtagttc
     1801 agcttttaag aagtcaccag tcaatccgtc aaaagctacc aatggatggt aaccataggt
     1861 ttggtagtgt gtattataat tcgtttgttc ttggtgacca aatgtatctg aatgggtcga
     1921 atctaaatca atgattaatt ccgtatcatt acggatgagg cgtgctttat cgataagttc
     1981 ttggttcaaa gcttgaagtt catggatatt ctcctcagat agtcgatcta aaaatcggga
     2041 aagtgaagat tgagaagcga gttctttcct atctaaaaca gctttaaaca caggatcttg
     2101 tctcaggaga ttagctgcag aatcagctga gtaaccagca attaattgca taatgaactg
     2161 ctcaagtatg gataagttgt catgagtaaa atacgctcgt ttgtcttcaa tgtgaatgtg
     2221 ttgcttagcc agttcagaaa aaccaaagat gttcatcagc tctttcacta ggacgagacc
     2281 cgaatcactc gataattgac cacctgtatg agaaatagtg atatttgaat tgaattttac
     2341 ttggtttttg tgtaagctag tcatgaaaag aactcctttc ttttggttgg tttagtcact
     2401 ttaaccatag cagaaagggg ttcttttttc atcacttaac gggtgaagat gaaaaagtaa
     2461 ttgtaagctg ccgtgaggca gtttcacatg gttttaatta aaagtatgaa ttattcagga
     2521 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc
     2581 cgaccaaagc aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata
     2641 gaattagcag aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact
     2701 catgatgaga tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca
     2761 aaagatcaaa cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa
     2821 gaaaggatag atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct
     2881 caaacattta cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat
     2941 agattatcat tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt
     3001 gaaaagaaat atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac
     3061 gttaaagaaa tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac
     3121 tttactttag ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg
     3181 ttttttcttt agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt
     3241 tttctgaatc ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac
     3301 agcaatatta gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga
     3361 atagttcttt tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt
     3421 ggaagatgcg aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta
     3481 gttaaaaaat ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata
     3541 attactttcc tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca
     3601 agtacattac caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca
     3661 gcgctgcaag tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct
     3721 gactttaata aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt
     3781 aaaaagttct gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc
     3841 gacacaaacc aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct
     3901 gaatccagca cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc
     3961 ggctgaacaa agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac
     4021 ggcactccca ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt
     4081 gtttttaata tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc
     4141 tgcagcacct ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca
     4201 aattgctgtt cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg
     4261 ccccaatttt ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt
     4321 agttccttta cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct
     4381 actgtaacat acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc
     4441 ttgaatagta aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga
     4501 actgttttca aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat
     4561 tgagaatttt tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat
     4621 ttccttttgt gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt
     4681 taaaatattt taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt
     4741 tagagggtac ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta
     4801 tggctattca agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg
     4861 cacatatatt ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa
     4921 acgtactatg gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc
     4981 aaaaagaatg gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta
     5041 aagaaaaagt agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag
     5101 tattccacat cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt
     5161 attttcaagg tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa
     5221 tttccattgg cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt
     5281 atcttcttat tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac
     5341 atgtgttaac tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc
     5401 gttttcttga tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt
     5461 tagatattgg tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt
     5521 ttttaatcac tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa
     5581 gctatgaaaa atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca
     5641 tagaaagtct taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata
     5701 accgtataga ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg
     5761 ataatattca acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat
     5821 ggctaggttc ttattttgtt atggataata gaattagtct aggtcaactt atcacataca
     5881 atgctttact agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa
     5941 tgcaaacggc tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa
     6001 aatcgaacca gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg
     6061 taaaaaacgt ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta
     6121 caattccttc gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc
     6181 ttgcaaagtt atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca
     6241 ttaatcactt agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag
     6301 agtcattttt ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac
     6361 caagtttcga acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc
     6421 aacagccatt acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc
     6481 aacgacaacg attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag
     6541 atgaagcaac tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca
     6601 atttaaaaga aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg
     6661 atcaaatttt agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc
     6721 gttttgccga tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg
     6781 cagaataata attggttcga acattctagt atctatacta aacaacatca tagcttctac
     6841 cgttgggtgt tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt
     6901 gctaaaaaag aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag
     6961 ttacaagtgc cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt
     7021 cataaggacg atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag
     7081 atagaacaag aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa
     7141 agtataagta aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa
     7201 ttaaagagca tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta
     7261 aatgatcaaa agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt
     7321 gagagttcag atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat
     7381 caatcattaa aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa
     7441 ctaaataatg tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata
     7501 aatgaacaaa ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa
     7561 ttagttaaac caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa
     7621 gagctagaac aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa
     7681 gagaagaacc agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa
     7741 gctcctgtat ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc
     7801 aaaggaacag tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca
     7861 gcacttttac ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa
     7921 ttggatcaaa aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa
     7981 aattctactg ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact
     8041 caatcgtttc cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa
     8101 acatattggc aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga
     8161 atataggagg agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat
     8221 tattgttact atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa
     8281 atacaaccct aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa
     8341 cgttagaaga aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg
     8401 cagatacagg tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa
     8461 cacagctaaa gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt
     8521 cacctggggg aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa
     8581 agctagatat tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca
     8641 tttctgctgg ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg
     8701 tcattatttc tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga
     8761 cgtataaaag tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag
     8821 agaataaagt tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag
     8881 caaaaggaag aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg
     8941 atggtaacca agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat
     9001 tagattccct aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg
     9061 acacaactgg ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa
     9121 aggcaactac atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc
     9181 caaaaccaat gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact
     9241 ttgcctaatt atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta
     9301 atttgtatta gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg
     9361 gaatctgaca aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc
     9421 tattttgtac ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg
     9481 ataagagtag tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag
     9541 tgtatatgta ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct
     9601 ttcacgccca agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt
     9661 cgttcattag aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg
     9721 gagagaatta aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa
     9781 cagggatttt atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt
     9841 caactacttt aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa
     9901 gcgggaatgt tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa
     9961 taggtttttt agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt
    10021 tatctattat tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta
    10081 ttattgcggg attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt
    10141 gaaacgatta atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg
    10201 gaagacatgt agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa
    10261 gacactctct gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt
    10321 ctattaaata ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt
    10381 agagagttca ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac
    10441 tttactgttc ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt
    10501 tgagttaaaa ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt
    10561 tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg gtttggcacc caatgggccg
    10621 tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa
    10681 cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac
    10741 atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat agattattca
    10801 catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc
    10861 taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt
    10921 aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa
    10981 tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt
    11041 atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg
    11101 ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt
    11161 atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa
    11221 agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag
    11281 caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt
    11341 tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag
    11401 taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg
    11461 tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag
    11521 aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa
    11581 cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc
    11641 gcaattttag tagattcagg tacggatgtt ccagaagaat tgataaaaaa atacgagatg
    11701 tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt
    11761 acccctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat
    11821 ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt
    11881 gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa
    11941 cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt
    12001 ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc
    12061 agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa
    12121 tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag
    12181 cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg
    12241 ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca
    12301 aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa
    12361 gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg
    12421 gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga
    12481 taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta
    12541 gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg
    12601 caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag
    12661 aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc
    12721 gtcaaattca atttagccaa agaaggctat gacgtttata ctgcatatga tggggaagaa
    12781 gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct
    12841 aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt
    12901 atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat
    12961 gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt
    13021 cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt
    13081 ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag
    13141 ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg
    13201 acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca
    13261 gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat
    13321 tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg
    13381 gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat
    13441 tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg
    13501 ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac
    13561 aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat
    13621 caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga
    13681 aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa
    13741 tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga
    13801 taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg
    13861 ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt
    13921 cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt
    13981 tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag
    14041 aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga
    14101 taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg
    14161 caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag
    14221 gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga
    14281 agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa
    14341 attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga
    14401 tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg
    14461 attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga
    14521 attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat
    14581 cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt
    14641 aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg
    14701 catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa
    14761 ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg
    14821 tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt
    14881 agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg
    14941 tcgcttatta gaaacgcata ataacgtgat tttaagtatc accgatcaag gattggggat
    15001 tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc
    15061 aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca
    15121 tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct
    15181 gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt
    15241 ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc
    15301 ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa
    15361 agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga
    15421 atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa
    15481 gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg
    15541 aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac
    15601 aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg
    15661 tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg
    15721 aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta
    15781 acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa
    15841 tatgctacga aacattcgat caaactgaag aagtatagtt atatcctgtc acagcaaccc
    15901 tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa
    15961 agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa
    16021 gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa
    16081 agtttcccct atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc
    16141 agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac
    16201 agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat
    16261 atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt
    16321 gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt
    16381 ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat
    16441 ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg
    16501 gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt
    16561 ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac
    16621 caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc
    16681 gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc
    16741 gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc
    16801 ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca
    16861 gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta
    16921 tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat
    16981 ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat
    17041 gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg
    17101 aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat
    17161 acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa
    17221 attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat
    17281 gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagcatc
    17341 aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa
    17401 aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct
    17461 gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt
    17521 tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc
    17581 acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg
    17641 catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat
    17701 gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg
    17761 gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag
    17821 agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat
    17881 cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt
    17941 gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga
    18001 ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa
    18061 gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc
    18121 catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg
    18181 aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa
    18241 gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat
    18301 aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata
    18361 accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc
    18421 atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgctag cagcgtttct
    18481 ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa
    18541 agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc
    18601 ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat
    18661 acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg
    18721 tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc
    18781 ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt
    18841 tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg
    18901 acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc
    18961 tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg
    19021 tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc
    19081 gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa
    19141 aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat
    19201 gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac
    19261 gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta
    19321 catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc
    19381 tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga
    19441 agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat
    19501 tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc
    19561 agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg
    19621 gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc
    19681 aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac atttgttcag
    19741 aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga
    19801 cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct
    19861 tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc
    19921 aaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt
    19981 tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc
    20041 tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc
    20101 tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat
    20161 gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta
    20221 ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac
    20281 accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat
    20341 cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa
    20401 ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa
    20461 aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta
    20521 tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg
    20581 tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta
    20641 ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg
    20701 caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc
    20761 ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca
    20821 aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg
    20881 ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga
    20941 caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg
    21001 attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta
    21061 gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg
    21121 aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat
    21181 atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt
    21241 atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct
    21301 tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt
    21361 tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc
    21421 gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact
    21481 gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt
    21541 gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca
    21601 ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa
    21661 tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa
    21721 aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag
    21781 aagacgcacg cgcagctatg ttacacgggg tagataaatt agcagataca gtgaaagtaa
    21841 cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca
    21901 caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg
    21961 gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa
    22021 cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag
    22081 gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag
    22141 aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg
    22201 tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa
    22261 acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag
    22321 aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa
    22381 tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc
    22441 aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg
    22501 ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat
    22561 tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag
    22621 atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag
    22681 atgcaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa
    22741 cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa
    22801 accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta
    22861 aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag
    22921 aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa
    22981 tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag
    23041 cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc
    23101 gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg
    23161 tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag
    23221 gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg
    23281 ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag
    23341 ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca acttgcttat
    23401 ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc
    23461 caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat ttcagcttat
    23521 ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt
    23581 gttctttact tttgtctcac gct
//
LOCUS       DAFBYI010000039        23274 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFBYI010000039 DAFBYI010000000
VERSION     DAFBYI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.039"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(94..522)
                     /locus_tag="LLD04_001286"
     CDS             complement(94..522)
                     /locus_tag="LLD04_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648226.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(550..1188)
                     /locus_tag="LLD04_001287"
     CDS             complement(550..1188)
                     /locus_tag="LLD04_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648227.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1192..3174)
                     /locus_tag="LLD04_001288"
     CDS             complement(1192..3174)
                     /locus_tag="LLD04_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK5648228.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3354..4019
                     /locus_tag="LLD04_001289"
     CDS             3354..4019
                     /locus_tag="LLD04_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648229.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5954..9103)
                     /locus_tag="LLD04_001290"
     CDS             complement(5954..9103)
                     /locus_tag="LLD04_001290"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBK5648230.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9116..10315)
                     /locus_tag="LLD04_001291"
     CDS             complement(9116..10315)
                     /locus_tag="LLD04_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK5648231.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10305..11900)
                     /locus_tag="LLD04_001292"
     CDS             complement(10305..11900)
                     /locus_tag="LLD04_001292"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK5648232.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12484..13191)
                     /locus_tag="LLD04_001293"
     CDS             complement(12484..13191)
                     /locus_tag="LLD04_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648233.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13256..14092)
                     /locus_tag="LLD04_001294"
     CDS             complement(13256..14092)
                     /locus_tag="LLD04_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5648234.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14110..15519)
                     /locus_tag="LLD04_001295"
     CDS             complement(14110..15519)
                     /locus_tag="LLD04_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5648235.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15533..17356)
                     /locus_tag="LLD04_001296"
     CDS             complement(15533..17356)
                     /locus_tag="LLD04_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5648236.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18199..18525)
                     /locus_tag="LLD04_001297"
     CDS             complement(18199..18525)
                     /locus_tag="LLD04_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648237.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18571..18933)
                     /locus_tag="LLD04_001298"
     CDS             complement(18571..18933)
                     /locus_tag="LLD04_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK5648238.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18970..19185)
                     /locus_tag="LLD04_001299"
     CDS             complement(18970..19185)
                     /locus_tag="LLD04_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648239.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19312..19647
                     /locus_tag="LLD04_001300"
     CDS             19312..19647
                     /locus_tag="LLD04_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648240.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19723..21720)
                     /locus_tag="LLD04_001301"
     CDS             complement(19723..21720)
                     /locus_tag="LLD04_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648241.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21818..22093)
                     /locus_tag="LLD04_001302"
     CDS             complement(21818..22093)
                     /locus_tag="LLD04_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648242.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22325..23059
                     /locus_tag="LLD04_001303"
     CDS             22325..23059
                     /locus_tag="LLD04_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648243.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23098..>23274
                     /locus_tag="LLD04_001304"
     CDS             23098..>23274
                     /locus_tag="LLD04_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBK5648244.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYG"
BASE COUNT         7262 a         4615 c         3583 g         7814 t
ORIGIN      
        1 acaactaaaa atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata
       61 tgttttttct attttatata aacttttgga tacttaatac caaccggttg gattgtacca
      121 acatgagaat gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt
      181 tgcatttgct gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac
      241 acgactatag tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac
      301 ccatcttcca gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt
      361 tgacttaata atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta
      421 atagatttca ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac
      481 aaaacctatc cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct
      541 ttttgatttt taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt
      601 gactacgata actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc
      661 cttatttctt ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt
      721 caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg
      781 cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt
      841 atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc
      901 atattcaatc aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg
      961 cttctctctc ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact
     1021 gaactgatca ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc
     1081 aacaacagtc acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt
     1141 gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc
     1201 attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat
     1261 aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag
     1321 aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt
     1381 atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta
     1441 gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa
     1501 aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt
     1561 ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc
     1621 aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc
     1681 gctgagaata tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg
     1741 ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat
     1801 aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag
     1861 gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc
     1921 tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata
     1981 attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga
     2041 tacattgact aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc
     2101 tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata
     2161 aaatacatga tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg
     2221 tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa
     2281 caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc
     2341 gattttttca actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa
     2401 aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc
     2461 aatgattcct gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa
     2521 tacttgatgt aatgaaaagt cattattcaa caataaactg atttctttat tcatcaataa
     2581 gaaaaaggat gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata
     2641 attatatacg tctgtatttg aaataaccga atacgaatag ctattttcaa agtcttcaaa
     2701 aatattagtg gcataatcag tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat
     2761 gacccgttca taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt
     2821 tattttgatc gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt
     2881 ctcgtgagaa gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat
     2941 cttgaagttc caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga
     3001 aagctcagtt tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg
     3061 cccaataaaa tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc
     3121 atttgtccga tagagtacct gtttatttat actaaccaag gcaaaaccac tcatgatacc
     3181 ggtcactatg attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta
     3241 cttaaatgat tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaagt
     3301 gtataggtca tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca
     3361 ccggagaaaa aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg
     3421 aaaaaattgg tgtttccgat aaaacgattt ctagttggga aaattctcgt acaatgccag
     3481 actttgaaat gctgtcactt ttacatcata ctttaggtct gccgattgat tttccttcta
     3541 ttgaatcagc taccgaatca atagaaccaa cattgccaga tgaacctctt gttaacaaga
     3601 aatcttattc atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg
     3661 tcttttgttt ggtctatctt tttcttatca attatcaata caaaaatgaa acgatcgatc
     3721 gattcaatcc ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg
     3781 acaattccat cgaacgttgg gtatgtaatg atatctttgg ccatggtaca tggctgtctt
     3841 ttcacgctgg agaggtagaa aaagagaaaa accatgctgt aatcaaacac aaaggaaaac
     3901 acgtcatgaa cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc
     3961 tcagccaaga ctatgacgac gccttaaaaa gaagccctca ggagccagat aggcattaag
     4021 taggagatga acgtagagat agaaaaaatc aatgcacatg atcttatcca aaaaacaggg
     4081 gtacaaatta attgaaaaag ccttgagatg aaagttccta aataaaaaag ttcagaatga
     4141 taggtatttc tggcttatag tataatattt acgccacagc ctcccaatga ttctttttgg
     4201 aatcataatc aaggatggac attcacaaaa atattttgct cattcgatcc gaatagatta
     4261 acgaccaatc tagtaagtaa tatagattgg tacgtagtca agctgcacat aatttagatc
     4321 aattttagtt cttttttata tttctatgat ctgctcattt acttttatat gatggcttac
     4381 agtaacataa ataaatatga taatcgctgg tagtttatcc aagagtactc aataaaatac
     4441 aaaatggatt gttcaagcgc ctatcataat gataaaatga aacaaataaa cgatacaagg
     4501 aggaagagac tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt
     4561 aatctatggg atttcggtag catgggcatt tttttataga ggaatcacac caacctggcc
     4621 gttttatctc ttgttagtgg gaatattagt aggaaatttt gcccctcttc actatcgtac
     4681 gaactttttt ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa
     4741 cattattgaa atgacaatta cctgtataat cattttctta gttatattcc taaacgtctt
     4801 ctaataagaa tctcttaaaa ttagcttgtt acagttagtt acctagttta tataacacag
     4861 ttatcttatc ttccttatca acatcttttg ccaactcttt gtcttaaatt cgaaacatat
     4921 aagcagagct acgaacacca agactgtttc ggctctgttt tttgcatcaa attcgctctt
     4981 atatagctga taatctcggc tctttaaaag aaattatttt cgcttcattt tattataaaa
     5041 aggctaggaa ccttgatgtt atcaggttcc tagcctttat ttaagtatta ccccacagac
     5101 ccttccatat agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact
     5161 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
     5221 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
     5281 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
     5341 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat
     5401 cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga
     5461 gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg
     5521 agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca
     5581 aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa
     5641 accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata catttcactt
     5701 aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg
     5761 gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga
     5821 ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga
     5881 catctatccc tattttctac acttccactt gactaccatc tttgaatcgt acaattttct
     5941 taactcttct cgtttacctc aattcattct caagtggaat caactcatca tctaacattt
     6001 tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg
     6061 ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt
     6121 cacctggtag atattgcaga ttagaagcat gtaattcttt ctcagaaaca aaccactcat
     6181 tggaaaagac atgaatcaaa tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa
     6241 agatatcttc attactatct aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa
     6301 cttctttgac tccttctgtt ttattcaatt tagccaaagc tttttcaatt tcttctcgtg
     6361 catctgaact attcgcagca taagttccta gtaattgatc aatataagtt gaatcaatat
     6421 catacaactt agtggtattt tcactataaa attcgatcgt tgaaaaatct tctccttctt
     6481 tttctggttc ttcctctatt aatgaaccct tcacggtctc gtacacgcca atttgttctt
     6541 ccaaaagttg aatttgattg ttcaaagttg gagatttcac catgtcatca ttgtaatcgt
     6601 cataagtcac taacgattca taagcacgat tcaattcttg aaatgctttg acgtactcga
     6661 tacggatttc aagtggcgta tgttcatcaa tggattccat ggatgaaacc tcttttaact
     6721 taccatatgc ttgtttgaat ctttttttgg attcctgata acttggataa atcaaacctg
     6781 attcttcttt tgcttttgaa tacagttttg tcgcatcctc gacattcttc tccattgtcg
     6841 ctggcttacg aaacgtcaca atcaatcctt ttgtcttatc aggatacgta cgattcgtac
     6901 gtgaaaaagc ttggatcaat cctgcatact ctagattacg atccacaaat agggtttgaa
     6961 tcgtaggggc gtcaaaacct gttaatagac gatccactac aatcgctaaa tcgatttgtt
     7021 tcccaaattg tttgaattcc gctctttttc gtgccaaacg attattgatg tctccgttat
     7081 aacgctcaat gtctgctaat gaccaggaag tatcataata ctgattatat tcctcaataa
     7141 ttttttgcat ttcttcttgt tgggcacttg aatctcggct attttcttct aatgaatagg
     7201 taatagcgat tcgtggaaaa tcaggatcct ccatcgttcg tccttcacga atgagctgat
     7261 tgggaaactc ttttgttaac caatcaggct ctttcgtcat ctctttgatg gcccgataat
     7321 actttttggc catgtcaatg gaactcgtgg ttaagattgc agatttcgtt ggcctgccat
     7381 ttcgaaaatc aaattttgta taggcattat ctggacggaa cattttatga atgacttttt
     7441 ggatatgttc atctgcttca aaaacagctg ggtccaaata actttctttt ttgacaggtt
     7501 ccatttgatc aatcatacga ttgatctgtt ctgatgaata ctctgcatat gtttctactt
     7561 cttttaattt ccgatagatt ttattctcta gtgaagtcgg ctccacggtg ttttcatgct
     7621 ctacttgaaa tcctaagaca gagccatctt ctagcgcatt cttaatggta taggtatgca
     7681 agacctctcc atactgatca tgggttgtac gtgccaattg ccctttggct tgtttccgat
     7741 tctcaggaaa aatcggtgta cctgtaaagc caaaccaagt ggatttcgga aacatttttt
     7801 tgatttcttc catattttca gcacttaacg ctcgatgaca ctcatctact acaaacacga
     7861 tatgttggcc cattaatttt tggaagcgat tggttccttt tttctcttct tgtttctttg
     7921 catatttcaa cgccgcatct agtttttgac gggttgtaat gattacaaca tttgcattgg
     7981 catctgccaa cagggtttca cttaattctt tggcacttcc cgttccaacg atcaagctat
     8041 tcgctttggc gttcccggaa gaaatcccag tattaaattc cgaagcaaat tttgtaaact
     8101 ctgtggtggt ttgattatct aaatctttgc ggtccactaa catgatcgtt cgatcgatcc
     8161 ccgattttcg tgctaaaagc ttagtagata caaaactagt caacgtcttt cctgaaccag
     8221 tcgcatgcca cacataccct gattgatgtt tatttgcggc aataaacaag gcttgaatcg
     8281 catgtacttg ataaggatgt aacaccatga gggttttatt atcttgatct tcactgacga
     8341 ttgtataatc cgcaatcaaa cgatgagcat ccgggatatt taacacttgt ttacaaaact
     8401 catataagtt ttctactttt tcattgtctt tcgttcgcca actaaataaa aacttagggt
     8461 gcaagtcttt cggtaaggca tttgcaaaat accgagtggt ttgttcattg gaaaccacaa
     8521 acaactgtaa ggtcgaaaag atattgttcc gaaacatacc ttcttctgca tattttttga
     8581 tttggttaaa tgcctgataa acaccatctt ttgcagttac ttgtttcagc tcgatttgga
     8641 caatcggtaa tccattgatt aataaggtga cgtcaaaacg tcgatcccgt gcttctatgt
     8701 tacttccacg ttttgcaatt tgatggacca cttcataagt agagattcct ccaccaatgt
     8761 cttgattgga ataaaggata agtgagacag aacccaattg cgaatcttcc cgttcaatcg
     8821 tgatccgagc catcccattt tcacctttta accatttcgc agcatcaaat ggggttttcg
     8881 ttcgcagcag taattccgtt ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat
     8941 tcagttctgc ttgattattt gagataattt tttggcgtaa attgacccaa aggtcttctt
     9001 ctgattttag atccggacga tacgtccact gattatgccc ttctccaagt acacgaatca
     9061 actgttcttc gacttctgct tcctcacggt gactaatttt cattcttctt ccctcctaga
     9121 caaacatctt ttgtaagaag ctttttttcg tttcttttag taaatctaac ttacgttgat
     9181 gaagagcgat cgtatcatct agctgtttga agaaagtacc gattttttgt tgttcatcct
     9241 ttacaggaac cttaacactg atattctcaa tatcagaact gttataagaa ggcaatgccc
     9301 ctacttggga gtatcttggc aaatttatag tttcaaacaa tattttacca aaatagatat
     9361 cccaagtatt attaaataaa taagccatgg tattgttatc tattaagaaa gggcttgtga
     9421 ccaaacgttt tctattaagc attattgctg cgccaacttt agcaaatata atcgctggca
     9481 catcttttat tggcttccat tttttacgct caatttgctc attagataca taatgatttg
     9541 catttctcat ttcatactca ttaccaatgt tattcatgtc ggaaacttta aaaaatggag
     9601 taccttcaga accaccttgc tcggcatcag gaaatccaat tcccgattga gttttaccta
     9661 attctcccaa cttacgctct tcccaatctt ccgtaaaacc aggaaaccgg atttctggca
     9721 cttttgctcc gttttcaggg aacatttttt gtaagaatcc ttttttcgtt tccttcagca
     9781 aatctaactt acgttgatga agagcgatag tgtcgtcgag ttttttgaag aatatgccga
     9841 ttttttgttg ttcttttaaa tcattgggaa tatagaatac tgtatctgaa acttttttcc
     9901 agtctgaacg aggcattttt gttccagatg tatcatttgc agccttttga tatctatcag
     9961 cttgaataag agaataaata aagtccgagt cgctcttctt agctctaaaa acccagaaat
    10021 ccccaatcgc tacgcctgta aaagttgcct tgagccaatt ttttaaatat ggacgtaact
    10081 ttccatatag aatatcattt ggtaaaaatt caatcccttt tctattatca aatttctggc
    10141 tcacatcttt attcagtcta ccttctcctg ccacgatatc ttcaaattca acttttggaa
    10201 gtctactgga gtttgtactt ttattaagtc taacaactaa atcaattaac ttacgctctt
    10261 cccaatcttc cgtaaatcct gggaaacgga tttctggtcg tgtatcattg ctcatgtttg
    10321 aacacctcca gcgcaccttg taaccacatt tcgttttcag gtgttgtttg cagggaagag
    10381 atagcttcta aaagttcttt ttctagcact tgctgttctt gtcgtatctt cttgatgtca
    10441 tttccgacat gaaccatatc aattggttct tcttcttcga aagtatctac atagcgtggg
    10501 atatttaagt tatagtcatt ttctttgatt tcatcaaagg ttgccaaatg agcgtatttc
    10561 tcgacatctt ttctttcagc gtaggtttcg aggatttttt gaatgttttc ttccgatagt
    10621 ttattttgat ttttcccttt aacaaattca cgactagcat caatgaataa tacatctcga
    10681 gtttgacgat tcttcttcaa cacaatcacc gtcgttggaa tcgatgtccc aaagaacaag
    10741 ttcgcaggca taccgatcac agcataaata ctgccatctt ctagtaattt ttgacggatg
    10801 accccttctg cagcgccacg gaataaaact ccgtgtggta agacgatggc catggtacct
    10861 gtttccttca aatgatagaa cccatgaaga agaaacgcaa aatctgcttt ggattttggc
    10921 gctaatttcc cataacgatt aaaccgagaa tcatctaaga aagtcgtatc tgctgaccaa
    10981 ttagccgaat aaggtgggtt cattactact gcatcaaatg tgtaaggttc atctgttggc
    11041 cagtccttat tcaacgtatc cccattgcgg agattcattt cttcggcgtc gacaccatgt
    11101 aaaatcaagt tcatttttgc tagattgtat gtcgttgtat ttagttcttg cccatgatat
    11161 ttaacgttat ctgggtgtgt taagtagtta cgtacgttta acatcaatga acctgaaccc
    11221 atcgttggat caaaaacact aaagaatcgt cgttcctttt gatcgagcgt tacgatttgc
    11281 gccatcatat cagaaaccat atgaggtgtg taaaattctc cggctttctt ccctgcttct
    11341 gaagcaaatt gactgatcag aaactcatac gcatccccga tcacgtcccc atcgtgttct
    11401 aaaacatcta catcattcaa tttcttgatg acttcggtaa tcgtcacatt tcgttgttgc
    11461 tcatctgttc ctagtttttt tgattggaga tccacatcat caaataaccc attaaattga
    11521 ttgtaggtag atgccaattg cacaaaggct ttgtttagat cgttcaattg gaacgttgct
    11581 tgttttgctt gatccgctaa gacgttgaaa agatactctg gtgcaattgc atatcccaaa
    11641 atatcaacga ttgtggcaat cagatcttct tttgattctt catctgaaag taattcacga
    11701 tacaacattg tttgtttgct tactgtgtca tattcctcta aggattcatc tgctaaaaga
    11761 accacttgtt ccaacaatcg atcggataaa tatttataga aaatcagtcc caataaataa
    11821 tttttatatt ctgacgcatc cattttacta cggagattat ctgccgcact aaataatttt
    11881 tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt tgctatatac
    11941 cctcttattt tagcataccc ccttttataa aaagcaaaaa aaaaagaaag aaacttattc
    12001 ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag actgatcgtt
    12061 aacttatttt ttatatttct acatagttat ctttagaacc atttcagata catatgaaac
    12121 aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa atatacaaat
    12181 ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac ttatatatca
    12241 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
    12301 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
    12361 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
    12421 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
    12481 tttttaccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
    12541 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
    12601 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
    12661 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
    12721 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
    12781 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
    12841 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
    12901 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
    12961 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
    13021 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
    13081 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
    13141 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
    13201 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
    13261 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
    13321 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
    13381 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
    13441 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
    13501 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
    13561 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
    13621 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
    13681 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
    13741 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
    13801 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
    13861 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
    13921 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
    13981 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
    14041 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
    14101 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
    14161 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
    14221 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
    14281 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
    14341 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
    14401 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
    14461 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
    14521 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
    14581 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
    14641 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
    14701 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
    14761 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
    14821 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
    14881 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
    14941 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
    15001 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
    15061 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
    15121 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
    15181 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
    15241 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
    15301 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
    15361 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
    15421 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
    15481 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
    15541 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
    15601 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
    15661 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
    15721 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
    15781 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
    15841 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
    15901 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
    15961 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
    16021 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
    16081 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
    16141 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
    16201 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
    16261 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
    16321 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
    16381 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
    16441 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
    16501 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
    16561 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
    16621 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
    16681 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
    16741 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
    16801 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
    16861 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
    16921 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
    16981 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
    17041 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
    17101 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
    17161 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
    17221 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
    17281 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
    17341 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
    17401 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
    17461 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
    17521 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
    17581 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
    17641 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
    17701 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
    17761 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
    17821 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
    17881 tcaatagaat gatttgggct agtattctta aaaattaact agcaaatgtt taaactgatt
    17941 tatttctaat ctacatgtag taagtatttc aattccacca attatcaata aaaataccaa
    18001 acatatccat aataattttt tctttttcat ttcttttata ttgttataac agtgaataac
    18061 ataaaaattc ccgacaatca ttgtaatatt tttttgtcac ctgtattagt atttaattta
    18121 taggaaacta taaaaactcc ctagtgaatt tacacctttg ggagttcctg tttggacggt
    18181 atgcataata catttaaatc aatttaacat taacacctca gcatatggta tctccacata
    18241 tatacggtct ttctttggat agtactcaat atttatgttg atagcacttc cattgtctaa
    18301 tatagcttta gcccggtatg tgttatctcc attttcatga gtaatcgtca catcatcaca
    18361 tgatcgattt aagttgtact gttcttgtaa aatctgtgtt accaatggtt ttgatgactt
    18421 ctctaatcca gatgtacctg tcatttttga aaaaataata cctataatag ccaagacaac
    18481 aatacctata atgacaaact tcttgttcat atttacttta ttcaactttt tatctcctat
    18541 ctccaaaata atttgttctt atccctctta tcagtacatt gacatcccta aaagtacacc
    18601 aaagattgct ccagctaccc ctgctatcat caaaatcgta cccattttct catctttttc
    18661 tcgatataag tagcccataa taacagcaac agcaccaaat acaacaggaa taaatgctaa
    18721 cgatactacc atagaaatcc aaccaataat caaatacagc ttccctgtct cttgtccatg
    18781 tacgatttgt tccttcattt cattaattgt ctgctcagta cttttcttta ctgtgtctgt
    18841 tccggttgtc tcatctgttt cttgtagtgc tccacaaaac tggcaatact tactttcatc
    18901 tgttatttct ttgccacatt ttctacaata catgtcttct cctcctaaaa tcattcaatt
    18961 gcttattagt taactgttta ctttattaaa tacatcagaa aacagctcat tcattggtac
    19021 acttaatatc tcagaaagca tcttcatttg atagggttta ggaatcgttc tccctgtttg
    19081 ccacgaactt acacattgct gtgtaacatt catttttttt gctaactccg tttgagaaag
    19141 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
    19201 ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa acttcaaata
    19261 ccattacaaa tacaacaact tgttgtacta tactgagatg ggggtgtatt aatgaaacag
    19321 aaacaatatg caaaggtcat tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa
    19381 ttagctgaaa aaatcttctc tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca
    19441 gagcctaacc tcgatagctt aacagctctt tctagagccc taggaacccc tgtaagccat
    19501 tttctagaca atgttgtcgt agattatgag gaagagtttt tggctctcta tcgttctctt
    19561 agcactgaag atgcagttcg aacaattgac tatatgaagc tattaaaaag gcaagaaaat
    19621 gaacgcaatc agctattgaa agattgaaaa ataaataacc caccgaactt taacggtggg
    19681 tgtattgaga tgaaataaga ttaagagcta tggataacct gtctagttta atacaattgg
    19741 gtcttttctc ttccttactc tccgtccatc atctcttgtt acacttgtaa attgatacag
    19801 aggaactatc ccagctcctg ataatatcaa ttcttttatt gtttcatcta tccctgcccc
    19861 tttcgctaaa tatattaaaa aactactact tgctttcaca ttgtatagtt tcttaatatc
    19921 cttagaagct cgttcaagag tgaatccttg tttaacaaag tagcctctgt tttttattaa
    19981 agcatttttc acgctgttat ccccaacatt acgtaggcgt tcacaaacag tgttaataag
    20041 ttctttctct gtcattagat atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc
    20101 ttcatctaaa atcaattgtt tttccttgct gagttctgat cgggaatttt gaggaaatac
    20161 tcttttagcc tctttttcgt taaaacatat cttaacccat tcataagaaa aatattgata
    20221 tgtaaaatga attttttcca gaagtggaag gatatcttca ttcaattttt gaatcacaga
    20281 agttgcatta aaaacgtcta atcgatagac attgcttctc ttatgattaa agtttcctaa
    20341 cgctttctct tcctttaacc tatttaggta ttcgagcatc ttcaaaggaa catcttcatc
    20401 atctaatttt tttatcaatc ctataaaagt cataagtgat agtaacttat tacatttatc
    20461 tatgtcataa gttttaccat agtaagaact cattttttta gctaattgtt cccccgacaa
    20521 aatacaagtc acactttcct tactatcatc aaaaaaatta acatcagaaa gcaagacatc
    20581 atagacacgt cgcatttcct ctagataatt ccttattgtc ctgaaaagtt tgtctttcat
    20641 tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa aatgtgtagg
    20701 atacaagtct aggttctcta atagccaatt aaatgtttct acccgactgt ctttctttaa
    20761 aattagcatc acaactccaa taaaattttc actgatatca caattactgg aatgacaata
    20821 aaaaatggaa tgcccagttt tactggtaaa aatactagca gagggattat gatcgttgtg
    20881 aaacagacat cttaagtttt tacttgactt aagtaattta ttcatatcaa tggttaacaa
    20941 tctttcataa atctcattaa aagacatgtc ggaggaatca aacaggcagt ctttaaacca
    21001 ctgtttcatt tcttctttgt cgtcattttt tatcaactta acaaaatcgg tttcattgac
    21061 tagttcatac tctctccctg aatcactttg acaattttta gaacatgtca tggtaaaatc
    21121 aatcggaagc tcagtaatat ctaacatgtt gtcttcatta attattatca ctgcatctga
    21181 cttatatcct ccaaaaaaca ttctattcga agcatttaca ttgtcatcac acccggggat
    21241 gttctttttc aagtattcgt aaacagcaac cattttacca tagtcagtaa ttgcttcttt
    21301 taatagaaag acaatcttgt atttttctag attttcagag taccgaatac ttttttgtat
    21361 cgcaaacgca gaattatgaa ctaaattgat agataaaacc ttttctatgc ttaagtatct
    21421 actatcatct ttactcaaat ctactacctt ccccttctca tctttaacag tgttatcgac
    21481 atccagaata attactctgg tttccacaaa actctccttg tcattgcctt tattctttgt
    21541 cttgcttgga ataaatgatt gtgatgaatt tagtatctct acaaactcat ctcttgtaac
    21601 ttctctatag ttatctaaac acctatctct tacatcagtt ttttcttttt ttgtaactgg
    21661 cttctctaat aatttttctt gatctacata aattggaata ttaatcattg atgtttccat
    21721 ttgtgtttcc tccttataaa attctgttaa agactaaaac atacagataa taaaaacaaa
    21781 ctctttatta ctatatgttt tagtcttatt tgtttactca acctttatac cgttccatga
    21841 aactatcaat atcagatttt ttaaatctaa tgaccgtatc aatttttata acaggtaacc
    21901 cgtattgaga aacatatttt ctaatcgttc ctactgatac acctagatat tcacttgcct
    21961 gctgatagtc catatactct agagtgactc cagaaataat gtcttctagc ataggcttta
    22021 gcactgtaat tatctctttc acttgttgtt caacttgtat agaaacaatg ctatcaatga
    22081 tttgtagaga catatttccc cctcctttcg tgattgcttg aacaaatttt ctaagaatta
    22141 tcttcgtatt ttttgctcaa aataaaagta ttttgttatt aaccgattaa tcaaacaaaa
    22201 aatgattacc agttcatcga catacacata atacctcgat aaaaaagggg tgtcaacagc
    22261 tttgtaaaat ttatttacaa cacccctttt ttgaggtata ctaattttag gaggtgtggt
    22321 caatatgata aaacgtccac caataaatta tttagaacga aaaaagatac tcggaacaaa
    22381 aataaaagct ataagaaaga gtaaaaaatt aacacaacca gcatttggtc ttatgattaa
    22441 caacggtcaa ttaattgaca aaaaaactat ctacgaatgg gagaagggaa cctatttacc
    22501 aataccagag cgattatccc gaattgctga tttgggaaat atgagtatag aggagttagt
    22561 ttgtggcaat gtagaagaat atattttggg aattattctt tatagagata gcattgtact
    22621 agatggtata acatttccag ataaaaacct gttccagcat cttcgtcaac agttcccacc
    22681 agtacactct aatttagata catggctgga tagatattcc aaattagaac cagagatgca
    22741 agaatttatt gctaacaaga cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa
    22801 tatcttaaaa atagaagaat tattcatcaa tgctattgta gaagaatttg acaataacat
    22861 tcttttcctc actagcagta tagaagaact actagaaaga atggtagatg aatggctacc
    22921 tattcaattg aaagacatgt cttatcccga agaagctgtg agagaaatta ctgataatat
    22981 aaacaagcta gaacaaacga tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa
    23041 gggaggtgat accatctagt ggtaagtact ccttgtaaac taagaggaga tgatttaatg
    23101 gcaaaaatat caaagtacaa aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac
    23161 attgccaccg atccacgaac tggtgaaaaa ataaatacta cgagaagagg ttttagtagc
    23221 aaacaagaag ctcaaaaaga ttatgacgag tacagacatc aaatattata cggt
//
LOCUS       DAFBYI010000040        23204 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFBYI010000040 DAFBYI010000000
VERSION     DAFBYI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.040"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(429..791)
                     /locus_tag="LLD04_001305"
     CDS             complement(429..791)
                     /locus_tag="LLD04_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648245.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(865..1245)
                     /locus_tag="LLD04_001306"
     CDS             complement(865..1245)
                     /locus_tag="LLD04_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK5648246.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1309..2502
                     /locus_tag="LLD04_001307"
     CDS             1309..2502
                     /locus_tag="LLD04_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5648247.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2579..3163)
                     /locus_tag="LLD04_001308"
     CDS             complement(2579..3163)
                     /locus_tag="LLD04_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5648248.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3364..4362
                     /locus_tag="LLD04_001309"
     CDS             3364..4362
                     /locus_tag="LLD04_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5648249.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4474..4983
                     /locus_tag="LLD04_001310"
     CDS             4474..4983
                     /locus_tag="LLD04_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK5648250.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5013..5672)
                     /locus_tag="LLD04_001311"
     CDS             complement(5013..5672)
                     /locus_tag="LLD04_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK5648251.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5674..6438)
                     /locus_tag="LLD04_001312"
     CDS             complement(5674..6438)
                     /locus_tag="LLD04_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK5648252.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6446..7123)
                     /locus_tag="LLD04_001313"
     CDS             complement(6446..7123)
                     /locus_tag="LLD04_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK5648253.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7160..8545)
                     /locus_tag="LLD04_001314"
     CDS             complement(7160..8545)
                     /locus_tag="LLD04_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5648254.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8755..8839)
                     /gene="ffs"
                     /locus_tag="LLD04_001315"
     ncRNA           complement(8755..8839)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LLD04_001315"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8862..9377)
                     /gene="tadA"
                     /locus_tag="LLD04_001316"
     CDS             complement(8862..9377)
                     /gene="tadA"
                     /locus_tag="LLD04_001316"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK5648255.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9484..10341
                     /locus_tag="LLD04_001317"
     CDS             9484..10341
                     /locus_tag="LLD04_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648256.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10422..10757)
                     /locus_tag="LLD04_001318"
     CDS             complement(10422..10757)
                     /locus_tag="LLD04_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648257.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10824..12194)
                     /locus_tag="LLD04_001319"
     CDS             complement(10824..12194)
                     /locus_tag="LLD04_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK5648258.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12272..12808)
                     /locus_tag="LLD04_001320"
     CDS             complement(12272..12808)
                     /locus_tag="LLD04_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5648259.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13117..14598
                     /locus_tag="LLD04_001321"
     CDS             13117..14598
                     /locus_tag="LLD04_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648260.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14701..15408)
                     /locus_tag="LLD04_001322"
     CDS             complement(14701..15408)
                     /locus_tag="LLD04_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK5648261.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15487..15825)
                     /locus_tag="LLD04_001323"
     CDS             complement(15487..15825)
                     /locus_tag="LLD04_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK5648262.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15936..16898)
                     /locus_tag="LLD04_001324"
     CDS             complement(15936..16898)
                     /locus_tag="LLD04_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK5648263.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16913..18244)
                     /locus_tag="LLD04_001325"
     CDS             complement(16913..18244)
                     /locus_tag="LLD04_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5648264.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18252..18932)
                     /locus_tag="LLD04_001326"
     CDS             complement(18252..18932)
                     /locus_tag="LLD04_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK5648265.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19151..19630)
                     /locus_tag="LLD04_001327"
     CDS             complement(19151..19630)
                     /locus_tag="LLD04_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK5648266.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19757..21436)
                     /locus_tag="LLD04_001328"
     CDS             complement(19757..21436)
                     /locus_tag="LLD04_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5648267.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21984..>23204
                     /locus_tag="LLD04_001329"
     CDS             21984..>23204
                     /locus_tag="LLD04_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648268.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQL"
BASE COUNT         6902 a         4780 c         3894 g         7628 t
ORIGIN      
        1 gcaactcatt tatcataact tatcgtttgt tatttgtcaa gaactttttt gaagtttttt
       61 tcgatagaat caagcttcgc aagtggttct tttttaacga ctcagttatc ttatcatgtc
      121 gttagtaaca tgtcaataac tattttttaa attttttaga aatgtgatat aacatccact
      181 tcagaatcaa cgctgttaaa tttatcatgt atttcttcct tggtcaacta taaattaagc
      241 attactttgt gaaatgattt ttcgatcaat tattattatt cgttatacaa ccgttttcaa
      301 catattttga aaataaaatg cactaaaaaa ctatctttag cagaacattt cattgtattt
      361 tttggaccaa acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa
      421 tctgtatttt agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt
      481 tgacctggtt cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca
      541 gtttcaaaga caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc
      601 aaactaatct tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct
      661 aaaccagtct ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa
      721 tggaccaaaa attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt
      781 ttgcttgcca aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc
      841 acattttcag aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa
      901 tgcttcttct atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt
      961 tttattcgtg aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt
     1021 tcttaaagat aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac
     1081 tggctgtccg acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga
     1141 aacatgaata agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat
     1201 ccctgtcact tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt
     1261 cgcatttatc attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt
     1321 cttaagatga tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg
     1381 gacgcaattg ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg
     1441 gacttcttgt ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc
     1501 tttggttatg ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa
     1561 caccatgctg tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt
     1621 ttagctgcag cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca
     1681 gtttatcctc cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct
     1741 ttgctagaaa aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa
     1801 gaaaacaatg taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg
     1861 agcaaagaag aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt
     1921 agtgacgaga tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg
     1981 attgatccta atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat
     2041 ctagctgcaa tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt
     2101 gaaaaacatt tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc
     2161 caagcggctt atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa
     2221 aatgttcaaa cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac
     2281 cctgaaggaa cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa
     2341 ttggagaaca ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt
     2401 ccttccggtc atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa
     2461 ggacttaatc gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa
     2521 aaggacgaga ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact
     2581 attctgaaat ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac
     2641 gttgtacatt tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg
     2701 tatgtcggaa atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg
     2761 ggaatccagc accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa
     2821 taaagaattg gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa
     2881 ggttgaacag ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac
     2941 ccattcctgt tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa
     3001 aaatcacgcc atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg
     3061 gtgctagttc agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt
     3121 ttggtccttt ttcacttttt aaatctagtt gtgggaatgc catattgttt cctcctaata
     3181 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
     3241 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
     3301 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
     3361 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
     3421 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
     3481 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
     3541 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
     3601 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
     3661 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
     3721 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
     3781 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
     3841 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
     3901 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
     3961 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
     4021 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
     4081 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
     4141 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     4201 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     4261 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     4321 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     4381 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     4441 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     4501 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     4561 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     4621 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     4681 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     4741 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     4801 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     4861 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     4921 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     4981 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     5041 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     5101 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     5161 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     5221 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     5281 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     5341 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     5401 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     5461 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     5521 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     5581 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     5641 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     5701 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     5761 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     5821 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     5881 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     5941 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     6001 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     6061 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     6121 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     6181 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     6241 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     6301 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     6361 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     6421 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     6481 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     6541 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     6601 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     6661 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     6721 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     6781 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     6841 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     6901 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     6961 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     7021 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     7081 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     7141 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     7201 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     7261 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     7321 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     7381 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     7441 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     7501 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     7561 cttttggaat atcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     7621 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     7681 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     7741 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     7801 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     7861 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     7921 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     7981 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     8041 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     8101 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     8161 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     8221 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     8281 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     8341 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     8401 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     8461 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     8521 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     8581 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     8641 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     8701 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     8761 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     8821 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     8881 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     8941 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     9001 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     9061 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     9121 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     9181 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     9241 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     9301 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     9361 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     9421 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     9481 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     9541 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     9601 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     9661 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     9721 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     9781 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     9841 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     9901 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     9961 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
    10021 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
    10081 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
    10141 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
    10201 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
    10261 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
    10321 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
    10381 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
    10441 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
    10501 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
    10561 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
    10621 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
    10681 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
    10741 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
    10801 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
    10861 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
    10921 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
    10981 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
    11041 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
    11101 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
    11161 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
    11221 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
    11281 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
    11341 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
    11401 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
    11461 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
    11521 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
    11581 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
    11641 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
    11701 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
    11761 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
    11821 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
    11881 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
    11941 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
    12001 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
    12061 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
    12121 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
    12181 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
    12241 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
    12301 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
    12361 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
    12421 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
    12481 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
    12541 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
    12601 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
    12661 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
    12721 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
    12781 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
    12841 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
    12901 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
    12961 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
    13021 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
    13081 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
    13141 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    13201 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    13261 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    13321 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    13381 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    13441 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    13501 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    13561 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    13621 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    13681 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    13741 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    13801 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    13861 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    13921 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    13981 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    14041 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    14101 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    14161 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    14221 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    14281 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    14341 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    14401 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    14461 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    14521 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    14581 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    14641 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    14701 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    14761 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    14821 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    14881 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    14941 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    15001 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    15061 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    15121 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    15181 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    15241 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    15301 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    15361 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    15421 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    15481 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    15541 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    15601 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    15661 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    15721 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    15781 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    15841 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    15901 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    15961 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    16021 ttcgcaccac ttgctaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    16081 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    16141 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    16201 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    16261 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    16321 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    16381 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    16441 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    16501 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    16561 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    16621 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    16681 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    16741 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    16801 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    16861 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    16921 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    16981 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    17041 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    17101 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    17161 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    17221 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    17281 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    17341 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    17401 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    17461 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    17521 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    17581 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    17641 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    17701 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    17761 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    17821 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    17881 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    17941 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    18001 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    18061 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    18121 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    18181 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    18241 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    18301 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    18361 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    18421 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    18481 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    18541 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    18601 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    18661 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    18721 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    18781 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    18841 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    18901 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    18961 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    19021 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    19081 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    19141 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    19201 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    19261 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    19321 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    19381 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    19441 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    19501 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    19561 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    19621 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    19681 ttcaaaaatt atttgttttt cgcaaaaaaa gagcaagacg agcactctat agagaggttc
    19741 atcttactct ttttgtttat tttttatttc gacgttgagt agcttccata atgactggca
    19801 agatcactgg gcggcgtttt gtttgttcaa ataagtaacg gcctaattga tctcggatgt
    19861 cttgctttaa tttgctccac tcaaaatcat tgctttctaa atgtttctca acgattgttt
    19921 caatcatatt gctgctttct ttcatcagat cacggctagc ttttacgtaa acgaatcccc
    19981 gtgaagtaat ttgaggtttt gaaatgattt tcttttctcg ccgactgata gtgacaaccg
    20041 ctacgaaaat cccgtcttct gacaagattt ttcgatcacg taaaacaatg tttccgatat
    20101 cacctatgcc taatccatca atcattacat tttctgcact tgtagttcct gcaacactca
    20161 tacgcttgtt cttatattcc agcacgtctc cgcgtccagt aatatagata tttttgtatg
    20221 gcatccctac tgcatgagcc aaatctgcat gagcagctaa ttcacgatac tctccttgaa
    20281 ctggcacaaa atatgttggt ttcatcaaat tgatcattaa ttgcaagtca ttaggattgg
    20341 catggccaga aacgcgcata ttctcagaga tcaacttcac tgttccacca gctcgataaa
    20401 tgatatcttc cgttttcgcc acgatggttt ccatcgcgat gcttggcgta gtagtgatat
    20461 aaaccaaatc tccttcttca atcttgacat tacggtgtgt accgtttgcc attttttgca
    20521 gagacttgat gggttcaccc attcggccag tttctaaaat gatcagttct tctggatcat
    20581 agtttttcat gtctttcaac gtaacaagca gatcttcact tggtaattga agtttttcta
    20641 aacgcatcgc tgtgcgaata attcgttcga aatcttgacc agtcaagaca acttttcgtt
    20701 gtgctttttc tgctgcatcc aatacttgtt gcactcgttg caaattactt gctacacaag
    20761 cgacgatgat acgcccttcc caatatttga tcgtatcaaa tacttcatct gctatttggg
    20821 cttcagaagc tacaggctga ggattttctg catttgccga ttcacttagt aaggctaaga
    20881 ctccttcttt gccgatttcc gccaatcgtg cataatctgt ttgatacatt gggatagccg
    20941 tttggtcaaa tttaaaatct cccgtataaa cgatactacc ttcttttgtt ttcagattga
    21001 tccccactga atctggtatc gtatgagtgg tacggaaaaa gctcactact gtttcaccaa
    21061 agtctatttc tgtatgctca tctaccacat ggaaatcctt aaatcctttg gctgcttcat
    21121 tacgactgac attcaatttt gctaactcga tcgtcaactc tgtcccgaat actggtacag
    21181 aaatttttga caacaagtaa ggaagtgccc cgatcgcatc tgcatgtcca tgtgttaaaa
    21241 agacaccagc tacacgatca atgttttctt ctaaatacgt aaaatcaggg attacgacgt
    21301 cgatccccaa caactcattt tcaggatatt ttagcccaca atctaaaata aaaatatcct
    21361 cgcccacttc tgcgacatac atgttttttc cattttcacg aacgccgcct aatgggataa
    21421 ttttaatggt actcacaaac ttcacctctt cttatgacat ccaagtttaa agatgtcgat
    21481 tttcttcttt atctaaatat atgagccatt ccaatgaagc atatgcaaaa tcactatttc
    21541 tcaacttatg gagaatcagc aggaaaatta ccagcacatc cgcgttctga cagttggatg
    21601 ggcattttat tttggatata cttcattaac aaaatggatc ttttgcgaaa cgctatagcc
    21661 agaatactta tataagtatt cagctgttcc ccattattat acaacaaata aactaaatcg
    21721 ctagtatcag caattgttag aacaactatg cacgtaaaaa tccctctgta gcatctagga
    21781 tttcttttgc tattcctctt tcatttcttc ttcgtcggtt tgaaacatac ttttttcatt
    21841 ttaccacatt ttttgcaata actatagtga ctttttatat atacagaacc tcatgttgat
    21901 ggtgtgcaaa cagcataaga tttaagctag ctttaagata ggttgtctat tatcaaatgc
    21961 atctactagg agggatgcaa aatatgaatt ttatgaaacg tgcatggaaa agtacaaaag
    22021 taaaatgggg acgttcattg ttattgtttg ccgtgttcac agccattctt atttttgtgc
    22081 tagctggcct cacgattcga agcgctgcag aagaagcagc gaatcaagca caaaaagaag
    22141 ttggagcaac agtgacttta agtgccaatc gagaagcttc atttaaaaaa caggaacaaa
    22201 cttcttcaag cggttctacc actgaagaac gtccagatcc aggcagtttt tctcttacac
    22261 cagtttcctt aacggacgcg gaaaaaatag ctgaattaga taatgtaaag agttattcct
    22321 ttgaaagttc cgcctcagct atggcaaaag atggaataac tgccatcact agttctgatt
    22381 caagtgaaga cagttcagaa actactgatg caggcggtga acctgctgga ggaatgggcg
    22441 ggaatccgca aatgactcaa gcagactttc aaatcagtgg tgtgtcagca actgcacaaa
    22501 atagtaattt ttctgatgga acagcaaaaa tcacggatgg tgaaggaata gatgacagcg
    22561 acaaaggcac aaacaatgta gtcattgatt ctactctagc agaaacaaat gacctggcag
    22621 tcggagatac tttcacaatc acgagtactg aagatgaaga taccacttat gaaatgacga
    22681 tcaaaggaat ttatgaatcc agcgaaacaa gcggcagtat gggtatgaaa ttcaacttca
    22741 tgaatccgtc gaatacactt tacacttctt acacttttgc aaatgaacta aatgggacat
    22801 ctgacgataa cacgatagat tctgctattt acactctatc tgatccagat aaaatggatg
    22861 agtttctcaa agaagctgag aagctgatcg acacagatac atttagctta caatcaaatg
    22921 attccatgta tcagtcaatg ttggaacctt taaacaacgt ggcaagtttt tctaaaaatg
    22981 ttgttttact agtggctgta gccggtatta tcatacttac tctgatcatt atgattacca
    23041 ttcgggagcg tcggcatgag ttaggcgtct tgctatcttt aggagaatcc agaagtaaaa
    23101 tcattctcca attgtttacc gaagtggcta tttgtatgat tcttgcatta ggagttgctt
    23161 cgcttagcgg aaacgtcgtc gcgaatgccg tgggacagca gcta
//
LOCUS       DAFBYI010000041        22708 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFBYI010000041 DAFBYI010000000
VERSION     DAFBYI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.041"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(194..991)
                     /locus_tag="LLD04_001330"
     CDS             complement(194..991)
                     /locus_tag="LLD04_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK5648269.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="LLD04_001331"
     CDS             complement(988..2172)
                     /locus_tag="LLD04_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5648270.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="LLD04_001332"
     CDS             complement(2197..3444)
                     /locus_tag="LLD04_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5648271.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="LLD04_001333"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="LLD04_001333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK5648272.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="LLD04_001334"
     CDS             5424..5738
                     /locus_tag="LLD04_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK5648273.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="LLD04_001335"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="LLD04_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK5648274.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /locus_tag="LLD04_001336"
     CDS             6423..7067
                     /locus_tag="LLD04_001336"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK5648275.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="LLD04_001337"
     CDS             7082..7411
                     /locus_tag="LLD04_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK5648276.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="LLD04_001338"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="LLD04_001338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK5648277.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="LLD04_001339"
     CDS             8399..9223
                     /locus_tag="LLD04_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK5648278.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="LLD04_001340"
     CDS             9216..9563
                     /locus_tag="LLD04_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK5648279.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="LLD04_001341"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="LLD04_001341"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK5648280.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="LLD04_001342"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="LLD04_001342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK5648281.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="LLD04_001343"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="LLD04_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK5648282.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="LLD04_001344"
     CDS             complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="LLD04_001344"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK5648283.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15193..15987
                     /locus_tag="LLD04_001345"
     CDS             15193..15987
                     /locus_tag="LLD04_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648284.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15977..18019
                     /locus_tag="LLD04_001346"
     CDS             15977..18019
                     /locus_tag="LLD04_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648285.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18300..18449
                     /locus_tag="LLD04_001347"
     CDS             18300..18449
                     /locus_tag="LLD04_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK5648286.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18460..19974
                     /gene="dltA"
                     /locus_tag="LLD04_001348"
     CDS             18460..19974
                     /gene="dltA"
                     /locus_tag="LLD04_001348"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK5648287.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19971..21176
                     /gene="dltB"
                     /locus_tag="LLD04_001349"
     CDS             19971..21176
                     /gene="dltB"
                     /locus_tag="LLD04_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK5648288.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21221..21454
                     /gene="dltC"
                     /locus_tag="LLD04_001350"
     CDS             21221..21454
                     /gene="dltC"
                     /locus_tag="LLD04_001350"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK5648289.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21457..>22708
                     /gene="dltD"
                     /locus_tag="LLD04_001351"
     CDS             21457..>22708
                     /gene="dltD"
                     /locus_tag="LLD04_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK5648290.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPD"
BASE COUNT         7108 a         4089 c         4694 g         6817 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt
    12661 ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc
    12721 acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct
    12781 aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct
    12841 ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa
    12901 tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca
    12961 gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca
    13021 cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt
    13081 ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg
    13141 atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt
    13201 gaatagacac tgaaatgata accatattca tccccccatg catctgcatt agcttttaat
    13261 tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg
    13321 aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg
    13381 tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac
    13441 atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga
    13501 agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct
    13561 ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt
    13621 ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc
    13681 ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga
    13741 tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca
    13801 gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc
    13861 ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca
    13921 aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta
    13981 aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt
    14041 tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt
    14101 agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca
    14161 gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg
    14221 gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc
    14281 agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc
    14341 aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt
    14401 ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca
    14461 ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca
    14521 atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg
    14581 atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt
    14641 tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag
    14701 tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt
    14761 tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg
    14821 cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca
    14881 taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa
    14941 tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga
    15001 tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt
    15061 ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa attttttatt
    15121 cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc
    15181 aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg
    15241 aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc
    15301 tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag
    15361 acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc
    15421 caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa
    15481 ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc
    15541 ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat
    15601 gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc
    15661 ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt
    15721 gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg
    15781 aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca
    15841 gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc
    15901 agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc
    15961 ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg
    16021 aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag
    16081 cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg
    16141 tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc
    16201 caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat
    16261 gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc
    16321 ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt
    16381 tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga
    16441 tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat
    16501 atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat
    16561 gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg
    16621 atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct
    16681 tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta
    16741 tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta
    16801 ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg
    16861 gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac
    16921 ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca
    16981 gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat
    17041 aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga
    17101 agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa
    17161 agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga
    17221 tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg gactgccaga
    17281 taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt
    17341 atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga
    17401 ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact
    17461 gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa
    17521 tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc
    17581 acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc
    17641 agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat
    17701 tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga
    17761 aaattatcac ctattgacta agctggggat tccacaaaga aggatcagac ggatgatctt
    17821 cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt
    17881 tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt
    17941 gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg
    18001 tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg
    18061 aataccattt atttggaata agaggttgtg acacgatttt tgtcacaacc tctttatttt
    18121 ttatattttt taaaagtacg agaaattata aagaaaaaag cagtgatttg atgtgctatt
    18181 tttataaaag ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca
    18241 tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa
    18301 tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa
    18361 ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag
    18421 gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt
    18481 gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag
    18541 acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa
    18601 aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc
    18661 tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc
    18721 aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg
    18781 gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc
    18841 aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat
    18901 attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat
    18961 ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc
    19021 ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt
    19081 tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc
    19141 gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt
    19201 tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt
    19261 tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca
    19321 cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt
    19381 gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg
    19441 aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt
    19501 attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg
    19561 ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa
    19621 tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg
    19681 attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc
    19741 gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa
    19801 aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa
    19861 gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca
    19921 gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac
    19981 ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac
    20041 cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga
    20101 cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga
    20161 ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga
    20221 aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa
    20281 cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct
    20341 cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa
    20401 catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac
    20461 caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat
    20521 atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca
    20581 ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc
    20641 atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt
    20701 ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga
    20761 caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt
    20821 ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca
    20881 tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt
    20941 tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt
    21001 atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag
    21061 agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag
    21121 tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa
    21181 tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc
    21241 aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt
    21301 tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga
    21361 gctggatatc caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa
    21421 aatcattcaa caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt
    21481 ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa
    21541 gtagatatct ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg
    21601 ttgaaaggag atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt
    21661 ggctcaagtg aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac
    21721 cagcgaaact atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat
    21781 atgatgatgc gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct
    21841 cctcagtggt tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa
    21901 cttcaaacat acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta
    21961 gctagaagat tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg
    22021 caaacgatca aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa
    22081 tggaacatgc tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag
    22141 caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc
    22201 attttagcaa atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac
    22261 gatttttata cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat
    22321 tgggattatc gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca
    22381 aaaaaccata acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat
    22441 accggactat ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat
    22501 agccaaggat tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg
    22561 gaagacacga tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg
    22621 tttttagaag aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc
    22681 aaatcttggc aacatcaaat accagata
//
LOCUS       DAFBYI010000042        22447 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFBYI010000042 DAFBYI010000000
VERSION     DAFBYI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.042"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            13..210
                     /locus_tag="LLD04_001352"
     CDS             13..210
                     /locus_tag="LLD04_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK5648291.1"
                     /translation="MNNRRRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            207..416
                     /locus_tag="LLD04_001353"
     CDS             207..416
                     /locus_tag="LLD04_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648292.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEEEEE"
     gene            413..712
                     /locus_tag="LLD04_001354"
     CDS             413..712
                     /locus_tag="LLD04_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648293.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHLYNHSAIIRIENTMECDKLLAKSKENLAVARLVDMEVINNGI"
     gene            702..860
                     /locus_tag="LLD04_001355"
     CDS             702..860
                     /locus_tag="LLD04_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648294.1"
                     /translation="MEFKIFEEDTRYELEEKLNEFAKNNEIQHISLTASKSGCTTYYA
                     AVVSYVSQ"
     gene            954..1370
                     /locus_tag="LLD04_001356"
     CDS             954..1370
                     /locus_tag="LLD04_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5648295.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKYGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            1609..1845
                     /locus_tag="LLD04_001357"
     CDS             1609..1845
                     /locus_tag="LLD04_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648296.1"
                     /translation="MYWRVYKMNNASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1869..2081
                     /locus_tag="LLD04_001358"
     CDS             1869..2081
                     /locus_tag="LLD04_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648297.1"
                     /translation="MDKESTREHYLSKFKRNNKPEEVFGSSVKEVGEKLTHLLKGEDL
                     TYDEAYASLQYCYNLLKYESNFIKVR"
     gene            2331..2555
                     /locus_tag="LLD04_001359"
     CDS             2331..2555
                     /locus_tag="LLD04_001359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648298.1"
                     /translation="MGEGKCEKRYDEDSEKRVVYELRANGEPDMYIGIFESVENAWKY
                     ARELEEQESYKSYSFEVYHRPYYKDIYFKD"
     gene            2644..2811
                     /locus_tag="LLD04_001360"
     CDS             2644..2811
                     /locus_tag="LLD04_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648299.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESDEV
                     IPLYGKRIAKH"
     gene            2837..3181
                     /locus_tag="LLD04_001361"
     CDS             2837..3181
                     /locus_tag="LLD04_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5648300.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3186..3467
                     /locus_tag="LLD04_001362"
     CDS             3186..3467
                     /locus_tag="LLD04_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648301.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3570..3884
                     /locus_tag="LLD04_001363"
     CDS             3570..3884
                     /locus_tag="LLD04_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK5648302.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3862..5556
                     /locus_tag="LLD04_001364"
     CDS             3862..5556
                     /locus_tag="LLD04_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5648303.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTFTNSSVENTGTGFTPLVF"
     gene            5576..6754
                     /locus_tag="LLD04_001365"
     CDS             5576..6754
                     /locus_tag="LLD04_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5648304.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            6717..7403
                     /locus_tag="LLD04_001366"
     CDS             6717..7403
                     /locus_tag="LLD04_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK5648305.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7403..8563
                     /locus_tag="LLD04_001367"
     CDS             7403..8563
                     /locus_tag="LLD04_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5648306.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8573..9448
                     /locus_tag="LLD04_001368"
     CDS             8573..9448
                     /locus_tag="LLD04_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648307.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIRIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9445..9756
                     /locus_tag="LLD04_001369"
     CDS             9445..9756
                     /locus_tag="LLD04_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648308.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9746..10099
                     /locus_tag="LLD04_001370"
     CDS             9746..10099
                     /locus_tag="LLD04_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5648309.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10089..10490
                     /locus_tag="LLD04_001371"
     CDS             10089..10490
                     /locus_tag="LLD04_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648310.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10483..10887
                     /locus_tag="LLD04_001372"
     CDS             10483..10887
                     /locus_tag="LLD04_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648311.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10899..11504
                     /locus_tag="LLD04_001373"
     CDS             10899..11504
                     /locus_tag="LLD04_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648312.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     TVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSVS
                     EDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            11524..11886
                     /locus_tag="LLD04_001374"
     CDS             11524..11886
                     /locus_tag="LLD04_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648313.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11889..12071
                     /locus_tag="LLD04_001375"
     CDS             11889..12071
                     /locus_tag="LLD04_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648314.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12089..15409
                     /locus_tag="LLD04_001376"
     CDS             12089..15409
                     /locus_tag="LLD04_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648315.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            15460..16197
                     /locus_tag="LLD04_001377"
     CDS             15460..16197
                     /locus_tag="LLD04_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648316.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            16207..18498
                     /locus_tag="LLD04_001378"
     CDS             16207..18498
                     /locus_tag="LLD04_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648317.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18522..20702
                     /locus_tag="LLD04_001379"
     CDS             18522..20702
                     /locus_tag="LLD04_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648318.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGTGALSVVDDGDEVVVTLDPNHKLELFKAKSQPNLLIGKTYTMSVEIMLEDDFTGDP
                     SKIGLRYTKMPNWVSELYTRNTLTTAKGVWQKLTGTVKITATSDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKVEYGSTATPYQPNLFDAPYYLSKAALGENIANKSVTFPIKSSDY
                     AVYDYKNIENFVVGEKYVATIKATKPATQVFEVRNTNSYGFGAMKPVEGLTDIWVLEF
                     TPTRIDAASPSSLQIYQSPKNTKGAVQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINEVDTTAKD
                     AQTKANANATAIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVGETHFER
                     QSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVDALQNRF
                     AASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD"
     gene            20780..21022
                     /locus_tag="LLD04_001380"
     CDS             20780..21022
                     /locus_tag="LLD04_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBK5648319.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21038..21235
                     /locus_tag="LLD04_001381"
     CDS             21038..21235
                     /locus_tag="LLD04_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5648320.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21246..22265
                     /locus_tag="LLD04_001382"
     CDS             21246..22265
                     /locus_tag="LLD04_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK5648321.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYGTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         7952 a         3326 c         4794 g         6375 t
ORIGIN      
        1 agttgattga caataaacaa cagacgccgc agagtagcaa aactaagaaa tcaagaactg
       61 aatgtagcga aagtaaagtt cgaacgggag tatggtgttt ctgcagaaga aacatataaa
      121 gtggcaagtc agtgtgttgc tgatgcaagt gatgctattc gtaagtttgg aatttcgata
      181 ctagatatta aatgggagga tacagaatga agatttatgt agtaaagttt ggaaatcaat
      241 tttacagaag tgatgaacgt tctatagggg ctaacacatt atccattgta gacatactcc
      301 aaagtgcaag atggtttgat aatctcgaag aagctaacca agtttcacga cgacttggtg
      361 gattaacgca agtatatgaa ctggtcactg tcgatcatga ggaggaagag gaatgaaact
      421 aaaagacgga ttttactcca gcagtcatgg tatcggcggt ttgatgctag atatgccgac
      481 aaagaaccct aaaacacgtg agaaaccaaa agtcaaagtc ggtgacatgg ttcgctgtga
      541 agcagaggag ttcatctatc cgtttcgtgg atatgtagaa catctctata atcactcagc
      601 gatcattcgt attgaaaaca cgatggaatg tgacaagtta ttagcgaaaa gcaaagagaa
      661 tttagctgta gcgagattgg tggatatgga ggttataaac aatggaattt aaaatctttg
      721 aagaggacac acgctatgaa ttagaagaaa aattaaatga atttgcaaag aataatgaaa
      781 ttcagcatat atctttaacg gcttctaagt ccggttgtac aacttactat gcagctgttg
      841 tgagctacgt aagtcaataa aaggaactcg acaaataaaa aagccggatc gctccgactg
      901 attcaataaa tccaacacat ttattatatc acataaagga gcggtttgac ttgatgcaat
      961 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
     1021 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattataa
     1081 cggatatgcc gaaggcaccg aaatatggaa acaaggcaga agacgcgatc attcagatga
     1141 tggatataga agcggagaga gatgcgattc tagcggcttt gatggctctt agtctgatta
     1201 gtcgtcagat actctactac agcttctgtg acgtaaacaa gcactctaat tatgaaatag
     1261 ggcaattgat acgaggatac ggtgaaaaga atgtagagaa gctgaaatcc atcgcgctga
     1321 tcgaatttgc agaagcatac aaaaaaggcg tgttagttca gtatcgttga ttttgtaggg
     1381 tttttgtagg gatagtgtag ggtttttgag tgttttaacg tgatattatg atagtgtcga
     1441 aagattagtg ataggtctaa gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt
     1501 taattaagct tcggtagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc
     1561 cttctaaatt gttcttatta tctatcatcc gttgctgtct attaatttat gtattggagg
     1621 gtatataaaa tgaataacgc ttccgaaaaa attgatcaac taactatttt agtaaaccaa
     1681 agaatcgaag atattatcaa agaattcgaa ggtgaaaaga ttaagaacat aaagcttact
     1741 ttaatggatc ataagttcga attaaattcg acaaaaagga agatcgttga gcttatagaa
     1801 gaagaaatag ctctccagat caatgagcaa caattagcta attaatttat gtattggagg
     1861 gaaaacgaat ggataaagaa agtacacgag aacattattt gagtaaattc aaacgcaaca
     1921 ataaaccaga agaagttttt ggatcaagcg ttaaagaggt cggagaaaaa ctaactcatt
     1981 tattaaaagg agaagactta acatatgacg aagcatacgc aagtcttcag tattgttaca
     2041 atttattgaa gtatgagtct aattttatca aagtaaggta attaattatg tcgctgtggc
     2101 ggaataggta gacgcttata ggaaagataa ggtttttgag ttgcaggcaa cggcttcgtt
     2161 gtgggcgctc ttacgaataa aaccttgcaa ggtgcaaatc cttgctagcg actttgttag
     2221 ggtaatgtac tgatgaagag tatctgcaga tatcggcgac gttcgcagag accccttttt
     2281 ttattaaaaa gatgttataa ttatttcaaa agaagaaagg agtgaaatag atgggtgaag
     2341 gcaagtgcga aaaaagatat gatgaggata gtgaaaaaag ggtggtttac gaattaaggg
     2401 cgaatggtga acctgatatg tacattggga tttttgaaag cgtagaaaat gcatggaaat
     2461 acgctagaga attagaagaa caagaatcct ataagagcta ttcatttgaa gtttatcatc
     2521 ggccgtatta taaagatatc tattttaaag attaaacgtt tttttaaatt gttaatttta
     2581 acacgggtta ctgagatcac tctttgagtg gtctttttat attgcacaaa ggaggtaaca
     2641 acaatgtata gaccgcaata cttagaacag aagtatgaag taatcactgt gcaaaatggt
     2701 aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata taaacgaaag
     2761 gaaagcgatg aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg
     2821 cgaaaggaga cggggcatga ccgaggaatt ctatagatgg ctattacagt tgataagaga
     2881 agatcgtttg gttaagttct accagtctcc taaatggcga aggcttaggg agaaagcgat
     2941 gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga
     3001 gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag atttagataa
     3061 tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt
     3121 agataaacaa gagaagaaga tagaaagctt tgctaacttc gacgcaagtg aaaggtggta
     3181 agtgcatgat catcaatgac aatggcagag agtatgatac agaaaagatt gaagagtatt
     3241 catcttatac acaaggatta attaaacgtt tgatatacgt tcgctatgta ggcatcaggg
     3301 atctgttatc agataactgt tgcagtaaat acaaagtgaa tcaagtaaga gaagcgttga
     3361 ataaagataa taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta
     3421 attattacat tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa
     3481 taaatcgcaa attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt
     3541 tcgaacttta tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct
     3601 acagagaaaa attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg
     3661 agccattctt agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg
     3721 aagaagatat atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag
     3781 cgttgcttgt ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc
     3841 ctgaattggt aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg
     3901 ttcaaagcta tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat
     3961 tatctagaag aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag
     4021 gattcaatca atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt
     4081 ctacaatgtt ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga
     4141 tatctttcat taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc
     4201 tttctactaa ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat
     4261 gaaagccaag caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat
     4321 ttagataaaa aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc
     4381 aaacttcgtt ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac
     4441 cgctttgatg aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt
     4501 ggtggtaaaa ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt
     4561 ggtccgcttg atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt
     4621 gacaaggatg gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat
     4681 aatgaagttg atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca
     4741 gatttaaaac ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga
     4801 cttacgttca tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct
     4861 tcatatgatg atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg
     4921 ataggaacag tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa
     4981 tacgataaag atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta
     5041 caaaacatca aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt
     5101 catggaaaaa atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa
     5161 tattatatta aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta
     5221 gaagaagcag gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta
     5281 aaagatctgg ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt
     5341 atgcgttggt attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat
     5401 gaaaaaatag aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg
     5461 aattttttag atgatattta tgattctgct cctgtaacat tcacaaatag ctcagtagaa
     5521 aatacaggaa ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg
     5581 gatttttcaa aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata
     5641 cttttgtaaa ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga
     5701 tttgattgca aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa
     5761 aaagaatgat ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga
     5821 atttagaaaa aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc
     5881 gtctaataat tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt
     5941 gaaagatgat gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct
     6001 agaaagtgat gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc
     6061 gtattatcaa gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa
     6121 cgctcgtaga tacgtactga aagggaattt attccgaccg caagacaata caacacaaga
     6181 tcaaatcaat aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg
     6241 tgcggtattt caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag
     6301 caattcaaga gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt
     6361 tcacgttccg aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc
     6421 ttttttaatg ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag
     6481 tctctatgaa gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat
     6541 caagattact agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt
     6601 atttacaatc aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc
     6661 agatgaaata tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga
     6721 cgacaatgaa aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc
     6781 agggatttat tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata
     6841 ttttggatag tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag
     6901 acgtatttca agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg
     6961 ttgtgattaa cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta
     7021 taaaaatcta caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa
     7081 atgtcgatga cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg
     7141 cttcatataa aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag
     7201 aatcgtttat ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg
     7261 cagaaaatag ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg
     7321 acgttaaaga aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat
     7381 ttggacaagg agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag
     7441 cgaccaattg atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt
     7501 aagcttatct aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga
     7561 tcaattagta ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta
     7621 taacgaagta gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt
     7681 atttgaacgt gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt
     7741 tgttaacaca actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg
     7801 gggtaaactt tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat
     7861 gaagcagttc aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc
     7921 agtatggtta gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga
     7981 acaagcaatt gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag
     8041 caaacaaact agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc
     8101 tgtaactatg ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga
     8161 tccgatttat cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta
     8221 ctggtctaaa gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt
     8281 attgccagta ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat
     8341 tggggtagcc tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga
     8401 tgaataccat tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca
     8461 acctaaacgt aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac
     8521 aactacaaaa ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta
     8581 tattctttgt cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa
     8641 tctgaagaaa ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat
     8701 tcctattttt tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga
     8761 cgacaaagag tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga
     8821 tcattcgcgt gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag
     8881 aattaatttg cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag
     8941 ttatctaagt cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat
     9001 ggcaaacatt gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg
     9061 cgcacagtgg gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc
     9121 taatcggcta tatcgctacc ttagagggca aaaaacaaat atacgaatct ggacagccga
     9181 gatgtgggca cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga
     9241 attagacttt tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca
     9301 caatgctgga gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc
     9361 tacgcctttt gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt
     9421 taaagcaatt aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata
     9481 gctgtgaagg ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg
     9541 atagctaaaa atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct
     9601 gaaagcttag aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta
     9661 gatgagtttg aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta
     9721 caagaatata ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa
     9781 cgatggttgg ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat
     9841 tggtgtagaa gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag
     9901 tgatataaca gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt
     9961 tcatccaatc gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg
    10021 ttacaacgtt atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt
    10081 gggtgaatat ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg
    10141 ctatcaaaac ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg
    10201 aggatgagct acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga
    10261 tcactgagcc aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata
    10321 gagaagattt aacaggagat tcactagata ttatttcatt gattcaaaat cgttcgattc
    10381 gttttcagag aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc
    10441 aattggtatt tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg
    10501 gagctaaaaa taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag
    10561 agcgaacgac tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg
    10621 attacagaaa aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa
    10681 aatagtcgtc cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga
    10741 aaatttgagt atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg
    10801 gacgaattta tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt
    10861 cgtcaattcg ataaattaaa taaataggag gaacaacaat ggctaaaaca acaacagtaa
    10921 caacttttga caatataagt attaaaagaa tttcttttaa ttttaagaat gcaacaaatg
    10981 caatttcaac tgattgtaat ggacaattag atggcgaaac agaaatgcaa acggtggtta
    11041 aaaaatgtgg agcggcagaa gtaaaatcaa aatctaaacc aatcaatatg acggtaacaa
    11101 ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa caagatggac
    11161 gtattaaacc aggcatttac tcttacggtc ctgattccgt aagcgaagat ttctcacttg
    11221 ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg ttagcatgca
    11281 cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag
    11341 aactagaagc aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg
    11401 cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta
    11461 aaaaaacttc agttgtggca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc
    11521 gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa
    11581 cctaaactga atatcaagaa attattgatg atcaatagag attttaacac agacgagttt
    11641 gcaaaaatgt cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc
    11701 gtatatgtag cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata
    11761 gataaatggg attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa
    11821 caagcacgtg atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa
    11881 aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg
    11941 acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc
    12001 aaatggctta caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc
    12061 agaaaaacta aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac
    12121 tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac
    12181 taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc
    12241 agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa
    12301 agttgaagta actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac
    12361 cgaagttcag aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc
    12421 taatgatata gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta
    12481 tgagtcaact atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt
    12541 agtagaatcc gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga
    12601 aaaattagca aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat
    12661 cgaagtgcta aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga
    12721 agctttagaa atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa
    12781 tgctgctaaa aacgttgata cctccacagt agatgatatc ggtaaaaaac ttgatatggg
    12841 taatttaatg gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg
    12901 tagtaagtct attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt
    12961 ctttggctct ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg
    13021 aatgttacca aatacgatca agcctgtttt tacacaatat acgtcaatgt ttaaaggact
    13081 tggatatgat accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga
    13141 tgcagcagct ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat
    13201 aaaagggaac tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc
    13261 agcttatgct gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga
    13321 agagtcattg ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca
    13381 tggtgaagga tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa
    13441 aataaatgaa gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa
    13501 gcaagcagtt cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca
    13561 ggctagtagg gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga
    13621 agatttttat gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc
    13681 aggagcgaaa gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa
    13741 aaatttcatt ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat
    13801 aaccgcagtt gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg
    13861 aattatagcg ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg
    13921 cgcaatcact gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac
    13981 ctgggaaagt ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    14041 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    14101 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    14161 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    14221 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    14281 agctggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat taggcgcagc
    14341 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    14401 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    14461 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    14521 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    14581 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    14641 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    14701 cagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    14761 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    14821 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    14881 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    14941 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    15001 aatcagtagt gtctttgata atttgaatat ttcgatacca catattccgt taccacattt
    15061 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    15121 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtcggtg aagcaggacc
    15181 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggggatc gtatcttgag
    15241 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    15301 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    15361 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    15421 tttctcctgt ttttttagtt ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    15481 ataatcgatg gatttcctga ttatttgcat aaattggctt tagcggaaag accaaccata
    15541 ccttctccaa aaagacagag agttgaaact tctgttttag gacggctagg tggcttagta
    15601 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaagatgtg
    15661 gaagaccatc aagcattcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    15721 aagaaattag aattctctga tgatcccaac gtctattatg ttatccagac tatcgatatt
    15781 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat cactgcgaaa
    15841 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    15901 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    15961 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    16021 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    16081 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    16141 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    16201 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    16261 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    16321 agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa cggaactaat
    16381 gcagatttga ttgtagacgg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    16441 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    16501 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    16561 gaaaagatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa accagctatc
    16621 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    16681 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggt
    16741 gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac ggatcataaa
    16801 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    16861 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    16921 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    16981 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    17041 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    17101 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    17161 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    17221 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    17281 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    17341 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    17401 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    17461 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    17521 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    17581 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    17641 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    17701 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    17761 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    17821 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    17881 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    17941 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    18001 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    18061 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    18121 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    18181 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat
    18241 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    18301 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    18361 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    18421 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    18481 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    18541 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    18601 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    18661 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttatgta gatagtgttg
    18721 caactagcgt aactagtgaa gaagtcatat tttctcttga tggcacattt atgagtgaag
    18781 caggatatta taaacactgc tacgtagaat atagaaaagg taatcaaatt ttaacaacgc
    18841 aagacatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    18901 acgtttcgca attggaagag ttgattcgaa agtataacga aacttttgat gcttttatgg
    18961 ctgaaattaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    19021 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaaa ttaggtaact
    19081 tgcaagtcat gtacagtaac agcatcgact tcgggggcta tgattatagc gggaatccga
    19141 atttaatggc taatataaac gctgacagct tctcgcaagg tactggtgct ttatctgttg
    19201 tggatgatgg tgacgaggta gtggtcacgc ttgatccaaa ccataaatta gagttattca
    19261 aagcgaaaag tcaaccaaac ctgttaatag gtaaaacata taccatgagt gtagaaatta
    19321 tgttagaaga tgatttcact ggagacccta gtaagatagg tctaagatat actaaaatgc
    19381 ctaactgggt atcagaacta tatacgcgta atacattaac tactgctaag ggtgtatggc
    19441 aaaaactaac tggcaccgtt aaaattactg ctacgagtga taacgctgaa agctggttta
    19501 taatgctaca aaataaagac gctaataaca gcctatctgg caaactacgt ttgagacacg
    19561 ctaaagttga atacggctca acagccacac catatcaacc aaatttattc gatgcaccgt
    19621 attatttgag taaggcggct ttgggtgaga atattgctaa taagtctgtg acgtttccaa
    19681 ttaaatctag tgattatgcc gtgtacgact acaaaaacat tgaaaatttt gtagtggggg
    19741 aaaaatatgt tgcgactata aaagcaacaa aacctgcaac acaagtattt gaagtaagaa
    19801 atacaaatag ttatggtttt ggtgctatga aaccagtaga aggtttaact gatatatggg
    19861 tattagagtt tactccaaca agaattgatg ctgctagtcc atcaagtctt cagatatatc
    19921 agtcaccaaa gaatactaaa ggagctgttc aaatcgactg gctcaagatc gaaaaaggcg
    19981 acacacgaac cccgaatatt gagcaatata aataccgagg aatcggcatg cgagactcaa
    20041 ataatccaaa agactatgtc tgggatttag caccagaata tgtagaaaac aatctggcta
    20101 cagatatcaa agttgctgaa attactggta aagcaaacaa ttataccgat gggaaagtat
    20161 cgcagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca gctaaggatg
    20221 ctcaaacaaa agcgaatgct aatgcgactg ctatagatga acgcattaat gatacagcta
    20281 ctaccgcatt ttatggcgca caaggtgaag tctctaacaa aacgaaagta gcggagtatg
    20341 gagtaggaac agggtatact actacagctg taggagaaac acatttcgaa cgtcaatcta
    20401 acggatacgt taaatgctta aaagctggta agtattcaat tagtgcgcaa atacgtgtgc
    20461 aattgggcgg gaaatatgct tcatggttat atactgattt atataaagat ggaacttctg
    20521 ttgataattt agtatcttat ggggtcgacg ccttgcaaaa tagatttgca gctagcggaa
    20581 acattgtaac tgatttagaa gtaaacgatg tactaaatat gcgtactgaa atcggcgttg
    20641 cagatggaat tttacaattt acatcttgcc gtactttagt cttgactaga ctatctgatt
    20701 aatacaatat ttataatttt gctatcaaca cgctcaaagg agggtgtttt ttattttgca
    20761 atgaaaggag gctagttggt tgaaagacga agcaatacaa gacgtggtag aacgcttagt
    20821 gcgtattgaa acgaaactgg ataattacga attattacgt gaaaaagcgg aaagtgcaaa
    20881 agatagagcg gatcaggcat attctattgc gcttaataat gcagaagaca tcaaagagat
    20941 gaaagccaat aataaatggt cgtggggtta catgatcggt ttaggcatta caatcattgg
    21001 ctatttcttg actaaattgt aaaggaggtg agaagaaatg gttttaccag ataagtatta
    21061 tcaagtcatt aaatggacag ttttaacagt attgccagct gcatctgtat tagtagcgac
    21121 gttagggaaa gcgtatggat ggaatggaac agatatgaca gtactcacta tcaatgcagt
    21181 agcaacattt ttaggtgtta tcactggtgt gtcggcttat aatttgaaaa aataggagga
    21241 aacaaatgaa aaagaaaatt actattactg cgatgagcct attaacggct ctttttttat
    21301 tgccaattaa tgggtttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag
    21361 gtagctcaca agtagcaaat aatcagtaca ttttactgca tgaaacggct aatgaaacag
    21421 caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagtgct tacactgctt
    21481 acattgtggg tgatggcgga attgtttacc aagttggaca acctggttat gtacagtacg
    21541 gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa cacacacata
    21601 ataaagcaac ttttgaaaag aactataaag catacgttga attggcgaga gattccgcta
    21661 agaaatacgg tattccgctt acattggaca cgccttataa ccaaccagga atcaaatcac
    21721 atttatgggt aacacaaaac atctggggcg atcatacaga tccttatggt tatctttctg
    21781 aaatgggcgt aagtaaagaa aaattagcat atgatttagc tcatggattt accgatgaaa
    21841 atccaacaac ttctgaaaac aagcctgtca ttgatccaac acgagctggt gcagctaatc
    21901 ctacactgac agatggaaca aactacgctc atattgatca gtttggggaa attgaaaatg
    21961 caaacttgca tgtagctgga tggcatattg ctaactataa atacgagtat attttcatta
    22021 tggattacaa tactgggaaa gaattagcta gagtaaaagc tgatggaatt tatagaccgg
    22081 atgtaaatca agcgtatggg acatctggaa atgttggcta tcatgtatct tttaacatgc
    22141 gtaacttccc taataagaaa gtctatgtca tgatgcgtgc aacgaatgat ccagaaggaa
    22201 acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac
    22261 gataaaaata gctcctcgtt gaggagcagt acataactat attgacaact ataaaaatta
    22321 ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccataaata gtcacactcc
    22381 aagctatgcg ataacaggtt tgttgccaca cattctactg gttgattgtt tatggcttta
    22441 tgtggca
//
LOCUS       DAFBYI010000043        22152 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFBYI010000043 DAFBYI010000000
VERSION     DAFBYI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.043"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            349..2391
                     /locus_tag="LLD04_001383"
     CDS             349..2391
                     /locus_tag="LLD04_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648322.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIVDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2402..3022
                     /locus_tag="LLD04_001384"
     CDS             2402..3022
                     /locus_tag="LLD04_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK5648323.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3034..3492
                     /locus_tag="LLD04_001385"
     CDS             3034..3492
                     /locus_tag="LLD04_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648324.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3511..3789
                     /locus_tag="LLD04_001386"
     CDS             3511..3789
                     /locus_tag="LLD04_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5648325.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3814..5181
                     /locus_tag="LLD04_001387"
     CDS             3814..5181
                     /locus_tag="LLD04_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5648326.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5196..6341
                     /gene="dgoD"
                     /locus_tag="LLD04_001388"
     CDS             5196..6341
                     /gene="dgoD"
                     /locus_tag="LLD04_001388"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBK5648327.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6368..6859
                     /locus_tag="LLD04_001389"
     CDS             6368..6859
                     /locus_tag="LLD04_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648328.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7302..8501
                     /locus_tag="LLD04_001390"
     CDS             7302..8501
                     /locus_tag="LLD04_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5648329.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8996..9754)
                     /locus_tag="LLD04_001391"
     CDS             complement(8996..9754)
                     /locus_tag="LLD04_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5648330.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9922..10857
                     /gene="pfkB"
                     /locus_tag="LLD04_001392"
     CDS             9922..10857
                     /gene="pfkB"
                     /locus_tag="LLD04_001392"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK5648331.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10854..12299
                     /locus_tag="LLD04_001393"
     CDS             10854..12299
                     /locus_tag="LLD04_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5648332.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12327..12782
                     /locus_tag="LLD04_001394"
     CDS             12327..12782
                     /locus_tag="LLD04_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648333.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12801..13775
                     /gene="lacD"
                     /locus_tag="LLD04_001395"
     CDS             12801..13775
                     /gene="lacD"
                     /locus_tag="LLD04_001395"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5648334.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13886..14447)
                     /locus_tag="LLD04_001396"
                     /pseudo
     CDS             complement(13886..14447)
                     /locus_tag="LLD04_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14591..15205
                     /locus_tag="LLD04_001397"
     CDS             14591..15205
                     /locus_tag="LLD04_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5648335.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15446..15829
                     /locus_tag="LLD04_001398"
     CDS             15446..15829
                     /locus_tag="LLD04_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5648336.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16192..16701
                     /locus_tag="LLD04_001399"
     CDS             16192..16701
                     /locus_tag="LLD04_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK5648337.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16801..17451
                     /locus_tag="LLD04_001400"
     CDS             16801..17451
                     /locus_tag="LLD04_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648338.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17902..18840
                     /locus_tag="LLD04_001401"
     CDS             17902..18840
                     /locus_tag="LLD04_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5648339.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18853..19905
                     /locus_tag="LLD04_001402"
     CDS             18853..19905
                     /locus_tag="LLD04_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5648340.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20171..20806
                     /locus_tag="LLD04_001403"
     CDS             20171..20806
                     /locus_tag="LLD04_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5648341.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20997..21788
                     /gene="msrA"
                     /locus_tag="LLD04_001404"
     CDS             20997..21788
                     /gene="msrA"
                     /locus_tag="LLD04_001404"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5648342.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7055 a         3509 c         4660 g         6928 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaaaagcat tgtttttagc agtttaatat tattcttttt
       61 caattagact gacttgattt agttacggaa tagatacttt aatgtgttga agaagcaacg
      121 tcccgtgttt atttactaaa gcatccaata ttctaccgaa tatttaatat tcacattagc
      181 cctaaaataa ttgtcatacc atgttacatt tgatataaca gcgttcattt taaaaatagc
      241 tgttagtgtg acaaaacatt aaaccaagtg tgacaaaaaa cgtgatgaaa gctttccgga
      301 aacgtatgtg aacaagtata taatgatttt gtcaggagga tagtcaaaat gaataaacga
      361 attcgtgaaa tactcaaact gattattaaa aaccctgaaa tgaaactttc cactttaaca
      421 agtgaacttg atttgacacg tcgtcaaatc aattacgcga tcaatcaatt taatgaagaa
      481 ttgaaaacaa agaacattcc gttaattcag agaagtcatt ctggaaatat tacggtacca
      541 atcgaggtta tccaaatgat atcacagcct gattacgaca tagttgatga tcaggaactt
      601 gtcgttctca ctgatagtga aagacaagca ttgatagtga tgactctgat cgctaatatc
      661 gattacatca gtctggatca tttggtagat gcggttgatg ttagtaagac gactgttatg
      721 gaagatatca aacgaacgga accgttcatc agaaattata gtctgacaat tcgatataat
      781 cggatgaatg gttaccaatt ggttggttcg gaacaccgta tcttacagct gttatcggat
      841 ctagtaaaac ggtttccgat ttttcgccga gaaatgattc gcagaaaatt ggcacaagat
      901 gtttctgaag aagaaatcgt ccatttgatc cataacatgg aacaaatgtt gcatcttagt
      961 tattctgatg agtcggtaga ctatttgcag acatcagcga gatttttagt aagccgtggg
     1021 atcaagatgt cagataagca cgcgtttttt cccgatgatg tgacggatac tcctgaattc
     1081 cgaatgctga tgatcttggt acatgaaaca ggttggagtc tatccaaaag ttatatggag
     1141 tggttcacgt tattattttt aacttccaat atttttgaga gcaaaactac tcaaaattat
     1201 gattcggatc aagaattgcg aaaattgatc agccaaatgg ttgaaaattt ccaaaatcaa
     1261 acgttgattt tgatcgatga ccgagaagac tttgaacgta ggatactgag tcatctgcgt
     1321 ccagcctgtt ttcgaatacg ctataacttg agtttaggag tttattcaat agatagtctg
     1381 atcaaggata gtaatcatgc gattttgatt gatttgatga aagaattgat tattcctatt
     1441 gagaattggc taaataaggc ctttccaaat gatgaattag atcttttatc ctattacttt
     1501 ggctatcaat taagtaatca taataaattg aataagcaac gacaaagggc tgtagttgtt
     1561 tgtactaatg gtgtcatggt ctcaaagtta gtacgagcca atatggaaaa actttttcca
     1621 gagatccatt ttttagcttc cctatctgtg cgtgatttct acaaatttga agctgattat
     1681 gatctggtct ttactacgac gccactaaaa agttctatgc tgcagtttat gatcgagcca
     1741 atcatgacat atcagcaaca aatcagttta cgatatcgag ttttgagtga tttaggaatt
     1801 actaaggttg atcgagcaat ggatgaattg ttaacaatca ttaggaaata tgcaaatatc
     1861 accaatccag tttcgttgcg agaagaactg cagtactttt tgatcaagga agatggaagc
     1921 acgccgctgg acaacttcaa tgtattgcct tcattgactt attacttgcg gccgagttat
     1981 gtacaaatcg ttgaagaaga gatgtcttgg gaacgagcag tggaggtggc ttgccgaccg
     2041 ttattggaac atcaaatcat tgactcgcgt tttatcgctg attgcttgga tcaaatcaga
     2101 caaccgggat atgcaggtta cttgggaatg aagacttgca ttccccatac aacggtcgat
     2161 aaaggtgtaa tcagtgacgg tgtcagcatt ttagtttgta aaaagccgat acgatttcct
     2221 aatggtgaag atgtcagctt gattttgccg ctttcatttt ttgatttaac gaagcatctg
     2281 cgagccatca atcaaatcgc tgatatcgcc aaagatgatt cattgattga gcagttattg
     2341 attgctgatg acgaaaagac agtttatcaa ctattaagac aaagatctta aggaggaaaa
     2401 catgagacta gcagattatc ctaaattgac agtgatcatg cgtggttata cttttgatca
     2461 ggcagatgct attttacaag caatgcagga atttgaccat caatttgccg tagagatgac
     2521 aatgaacaca aaaggagctc ttgaaaatat acatgaacta aatcaacgtt atggtgacac
     2581 gacttatatc ggcgctggga cggtaaggac attagaacaa gcccaagcct gctatgaggc
     2641 tggtgcaaaa tttttattag gaccacatat gttcacaaaa gaaatgttgt cttacgccaa
     2701 taagaaagga ttattggcgg tacctgccgc aatgactcct tcagaagtcg accgtatgtt
     2761 taaagaagga gctgatattg taaaagtatt tcctgcagcg gttgttactc cacgtttttt
     2821 caaggatatt caagcacctt taggtaagtt accactaatg ggggttggtg gaatcagtaa
     2881 ggagaatgcg aaagagtttt ttgaacaagg tgctagctat ttaggtcttg gttccggtat
     2941 gttcaacaag caagacatcg aagagctaaa tgttaaaaac ttagcacgtt cgatgaaaga
     3001 attaattgag gctattaaat aggagtgagg actttggaac tttatttgaa tgaggaactc
     3061 gtttttcgaa atgttcaagc aaaaacagat agtgaagtct tagcgttttt agcaagcgag
     3121 atgtacaaaa aaaactacgt gaaggaggaa tatattcaag caatccaaga acgggaaaaa
     3181 gaatacccta ctggattacc atcaacacca ccaggaatcg caattcccca tgcaaactat
     3241 gaaatggtca ataaaacaac tttagcaatt gcgactctta aagatccggt cctatttcat
     3301 aatatggaag ataacaatga gcagttacca atccaaattg tgatcatgat ggcgattggg
     3361 gaaccacatg ggcaagttga aatgttacaa aaaattgttg gaatcataca agatgagcca
     3421 ctgcggcaag aaatgatcag agcaggcaat gatactgaat tattagaact attaaagaaa
     3481 gcggtttttt aatataagga ggaaatcata atgaaacgaa ttttagtagc atgcggaaat
     3541 ggtatcgcca cttcaacagt agtagcaaca aaagttagag agtatttgca acaacatggc
     3601 attgatgttt caacgactca aacaaaattg atggaagttc caggaaaagt tcaagactat
     3661 gatctattgg tgacgactgg tcaatttgat ggtcaaacag gtggtgtacc agtaatcaaa
     3721 gggatgccga ttttaacagg tatcggtgcc gatcagacaa tggaagagat tttgaactta
     3781 ctgaaataat agctgatcaa aggagctgaa aaaatgaaaa tcattatgaa cgtctttaac
     3841 ttctttattg atgctggacc aacagttatg ttgccagtca tcatcacgat tattggtttg
     3901 atttttgggt taaagatttc tcgtgctttt aaatcaggtt tgacattagg tatcggtttt
     3961 gccgggatca agttgatatt ggactttatg acgaccaatg ttggaccagc tgccaaggcg
     4021 atggttgatc gaactggtgt taaactagat gctttggatg ttggctgggg ttcgattgcg
     4081 gctgttactt gggcatcgcc aatcattcca atcttgattt ttgccattct attggtcaat
     4141 atcgttttgc tgattttgaa acgtacacat acgctggatg ttgatatctg gaactatcac
     4201 catatggcaa tcgttggtgt tatagtgtat tttgtgacta agaatgtctt cttaggtgta
     4261 ggtgcatcag tagtgatggc aattgcgact tttaagattt cagactggtc gcaaccgatg
     4321 gtagaaagtt tcttcggaat ccctggtgtt tctttaccaa ctgtttctgc gctttcatca
     4381 ctggttattg cttggccgtt aaattggctg ctggatcgta tcccgttatt cagaaaatca
     4441 aaattcacca tcaaagatgc acaaaaatat ttgggatttt ttggtgattc tatgatcatg
     4501 gggttagtta ttgggatggt gattggtgtt ttagccggct atgatatcaa agcggtttta
     4561 caattaggtg tcagcatgtc agctgttttg gttttgattc ctaagatgac ttcattattt
     4621 atggaaggat tgatgccaat ttcagatgct gcacaaaaat ggtcacaaaa aaaattcaaa
     4681 ggtacacgtt tatttatcgg attggatgct gcagttgttg taggtaatcc agatgttatc
     4741 acaacagcat tgatcattat tccactaacg attgcattag ctttagtatt gccaggaaac
     4801 cgtgtgttac cattcgcaga tttagcggtt gttcctttcc gcgtggcaat ggtcgttgcc
     4861 ttgactcgtg gtaatttatt aaaaaatatc attattggct tagttgtgac agcttcattg
     4921 ctatggtgtg ggtcagctac tgcgccaatg ctgacagcaa ttgccaaatc ggttggtatt
     4981 aacttaggaa cttcttcaat gttaatctca tcatttgctg cgacttccat gatccaaagt
     5041 tatttggtat ttatcgcatt tgcttacaaa ccaatcatcg gtattccagt gttgttatta
     5101 gcctttgcgg gcgcatggta ttacttcgat ggtatcaaaa aagttaatca tgaaatggat
     5161 gaaccattga aggcacaata gtaggagggt ttaaattgaa aatttcagat gtcacagtgt
     5221 ataaagtaaa accaagatgg atttttgtta agatctcgac tgacgaagga atcgaaggct
     5281 ggggagaaat gatctcagga actaaaacag aaacggttgt tgctggagct tatgagatag
     5341 gaaaccggct gattggacgt aatccgtttg aaatcgagcg cctcttccaa gaaatgcatc
     5401 gatctttctt ccgaggcggt cctattaatg gaaccatcgt ttcgggttta gaaatggctc
     5461 tgtgggatat caaaggaaaa gcgtttaatg ttccggttta tgaattattg ggcggtgcag
     5521 cacgtgataa aatcaaggtt tattcttgga ttggtggaga tcgaccaagt gaagttgcag
     5581 aacaagcaca agatcggttt gatcggggct ttacggccgt caagatgaac gcgacagaag
     5641 agctgcatta tatcgattcc taccaaaaag ttgatgaagt ggtggaacga gttgcgtcta
     5701 ttcgcgaacg ttttggtgat gcaatggata ttggtgttga ttttcatgga cgggtccata
     5761 aaccgatggc aaaagtgcta gcaaaagctt tggagccatt ccatccaatg tttttggaag
     5821 aagtcgtttt acctgaaaat gaagaacact ttaaagaagt agctgattct gtggctgtac
     5881 cattagctac tggtgaacgc ttgtacactc gttggcaatt caagaacctc tttaaacaag
     5941 gtactgttga tattatccaa ccagatgtgg ctctttgtgg gggaatcttg gaaacccgta
     6001 aaatcgcagc gatggcggaa gcttacgaca tggctgttgc gcctcatgcg ccatatggtc
     6061 ctgttgcact agcagcaact ctgcaagtag actcatgtac gccaaacgtc tttatccaag
     6121 aacaaagctt agggatccac tataacaaag gttttgatct gttagatttt gtgaagaaca
     6181 aagaagtttt ccaatataaa gacggctacg tagatctacc ttcaaaacca ggtctgggct
     6241 tggaaatgga tgaagacaaa atcaaagaaa ttgcacaaga aggtttgatt tggaccaatc
     6301 cgcaatggaa aaattatgac ggtacgatcg ctgagtggtg ataaatgaaa ataaaggatg
     6361 atgaaagatg gcagaagcac ttatgatcac agttttttta ggactgctga ttattttgca
     6421 aataatcgat caaaagaaaa ttatcgtgtt tgctcagagt agtcctgtaa ggaagagttt
     6481 gagtatttta ctgacagtcg ccatcctgtt gattttctgg ccggaacatc tgactgatca
     6541 gattaaactg gttgcttttg cttctgtcat tttgatcaat ggttttttga aggagggtct
     6601 agcaaaagat tctgttgtta gatttggagt attgaacggt aactttaatc gatatcctca
     6661 agtccaagta gaaaaactag gtgacgataa atcatttatt tcattttaca aaagcaagaa
     6721 taattctttt tcaatgattt ttgataaaga tcaaactgaa atgatggatg tctttcaaga
     6781 acttgatatc cattccaaaa ttactttaga tcaaagttcg gaagaaaaaa gagcagttgt
     6841 aaaaaaagat aatgaatgat tctactaccg aaattatgat ttctttatca cgaataatga
     6901 aacttgtttt aatgaatcat attgattgaa gtatacaatg tatattaagt tttggaaaga
     6961 aatatattgg tacaagatat agcaggaatc gattactcga tccctgcttt ttttaacttc
     7021 ctttgcagca aaaaaagctc gtaatctaag agatgatgga tagtttttag aatatatata
     7081 ttttcttcaa ttccagaaaa aaatgaccaa gctagtatgc taaaaaagta cgatgcttct
     7141 atgatctttt ttccgtcttt attaacagat gttttaattg atcaacgcat attgtaatct
     7201 accctaattt ccatagttct tcaccacttt taaaaatagt aaaaccccta caagtatgct
     7261 atagtaaatt gctaaagaaa aaccaataag gtgggagatg tgtgcgcgat caaacaagcg
     7321 gaaaaatgat tcgtttttat tatacaagtt tgcattttct ttattggatg atgttttgtt
     7381 ctgtctatgg gtttgcgaca ttgtttttga tttatttcaa gattgatcca gctaaaatcg
     7441 gagggatctt agcattggtc aatattgttt caacggtgct tcagcctttt gtcagtcaag
     7501 tgatcatcaa gaaaagaaag atcccactga tcaccgtgct gaagacgatg atagcaggat
     7561 taggagtgat tctcttagct ggggtattga ttccgaattt gccgatcatt acttatatct
     7621 tgggtgggat tttagtagtt tctatgcaat catttctcaa tgcgctggca tttgagtatt
     7681 caaataccgg ctacaggatc aactttggct tttcaagagc tggtggttca ttttcttatg
     7741 ctgtaacttc ttttttactg ggacagctgt tagcgattta ttctgccgcg attttgccaa
     7801 tcttggttat agttgaagca gtgatatttt tcgttatttt gcatctgctg ccggatgtaa
     7861 aaaatcaaga agtttttcct atgacaaaag aacaggatcg ctcagtttct ttgagaaaaa
     7921 aatatccttt cctgattttg ttatttttag gtttcagttt tctttttact ttccacacca
     7981 tgatcaacag ctttttggca cagatctttg aaaatatcca cggcggcagt aaagaagttg
     8041 gcatcgcatt gatgatcgcc gctttatgcg aattaccggg aatgatcttt tttgaacagc
     8101 ttgtcaaaaa gaaatccagc aaattttggc tgtctgttgc cagtatttca tttgctgtca
     8161 gaggattcat gatgctgctg gccagttcga tcctgatgat ggaaatgacg catctcctac
     8221 aagccttcag tttcgctttg tttattccag cttcggctta tttgttcaat caatttctgg
     8281 cggatgatga tcgtgtattt ggacaaacga tgatcatcat cgccacaact cttggtgggg
     8341 tgataggaaa tgtcttgggc ggcagtctgc tgcagcattt cggtgtatgg gagatgctga
     8401 ttttcggtac tggttttgcg gtgctaggtg ctttattaac tattttgggc attcgaaaaa
     8461 taccgaagac aggaaacgat agtgattctc ttgttgagta gcataaaaat acttagatac
     8521 tagttttata taaataacat atagtgatta ttggcgtaat tacaaaaaac gagaataact
     8581 taagtaagca tttctaaata attgagaaac ctcatctaaa aagataagaa aatatcccta
     8641 ataaaacttc tgtaacattt tagataactg attgtaatct gaataataaa ctataaggag
     8701 acggttagac ggtctccttt ttttgcgtat tataattcta gaattgttgg aaattgataa
     8761 taattaacaa gaaatattag acaggaactt cgaactgctt atcacattct tattattata
     8821 tatttgcgta agtagtcctg gtagtctcaa tgcactattc tatcttgaaa ctaagaagtt
     8881 atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata tttttctaga
     8941 caaccttagt taattcacac agatatattt taggtacagt cttacagata tagagttagt
     9001 gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta gccagtatag
     9061 aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct gatttaaagc
     9121 caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca gtaagcaaaa
     9181 ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc tttgtttcac
     9241 cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta atgtttttta
     9301 tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat ttgaccatca
     9361 tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt ctagcagcct
     9421 tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg gtgcggatta
     9481 cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta tcaagagcgg
     9541 caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac gtaaacccct
     9601 catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg gcatattcac
     9661 ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata aaatcccaga
     9721 gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta gttaggataa
     9781 ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt ttgtaaaagt
     9841 tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac ataatgaata
     9901 tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt ggcaatcgac
     9961 ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa cgatgatgat
    10021 atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt aggtatcgat
    10081 agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga ttatttgcat
    10141 caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat caacgttttt
    10201 acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc tgagattcct
    10261 caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga cgattatttg
    10321 tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga tatcagtcgt
    10381 atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga aataattgat
    10441 tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca atcttggtat
    10501 aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt gattgccgat
    10561 ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt aaataaggat
    10621 atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg tgctggtgac
    10681 actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga tattaatttg
    10741 aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac agatttttcg
    10801 gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa aaaatgacaa
    10861 cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact tatatggctc
    10921 aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt gaaacccatg
    10981 gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct gacgctgtaa
    11041 tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt gtaatcgatg
    11101 tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg ttggctggta
    11161 aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa gcggagatgg
    11221 gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt gtttctcata
    11281 tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc atctggggtg
    11341 tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg ttaaagggtg
    11401 ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc gctgaaggaa
    11461 tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc aattctggcg
    11521 gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt atcttactat
    11581 tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg atcttcttat
    11641 atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca aatccaatgg
    11701 aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct aatccattaa
    11761 ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca gtcaataaag
    11821 cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt atggcagggg
    11881 tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg ttttttggaa
    11941 aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg ggctcaacgc
    12001 acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt ctgccaacta
    12061 tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc caagttcccg
    12121 cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca tgggtagggt
    12181 cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa aaacgtagtg
    12241 tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc aacaactaaa
    12301 ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa gtttttatga
    12361 ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag tcaattgtct
    12421 tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga gaagaagaaa
    12481 gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct agtatcaagg
    12541 atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt ttagatggag
    12601 aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga acaacccatc
    12661 taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca gaaggtctga
    12721 aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta gaagaaaact
    12781 aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat attcgtcaac
    12841 taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt gccttgaaaa
    12901 aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc aatttcaaaa
    12961 aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat ccagaatatg
    13021 ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc tatgaaaaaa
    13081 ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt tggtctgtaa
    13141 aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac gatgtagatg
    13201 aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt tctgaatgtg
    13261 ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac gatcctgata
    13321 cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg atgaaggaat
    13381 tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt aatatgaact
    13441 atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct gctgcttatt
    13501 tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca ggagtaactg
    13561 ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca ttcaatgggg
    13621 tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact aaaggtgaat
    13681 atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag ctgaataatg
    13741 ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat aaccttaagt
    13801 ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat tggaagcgtc
    13861 ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg tcgttcaata
    13921 agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc tacccaatga
    13981 tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat ttcttctgtg
    14041 atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc gatcagatta
    14101 tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt gtatacgaac
    14161 gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct atgtgtcgta
    14221 tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt ttcataaaga
    14281 tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc agtttcggtc
    14341 agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga taatgcttct
    14401 gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt gcagcctcct
    14461 aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta tctgaacata
    14521 gataaccgac accttgtcta ttataatata agtatagcat atcaatattt aaagaatagg
    14581 agaataagtg atggaattag cctttaagca actggaaaag aaagactaca aaaaagccat
    14641 tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac tccttttgaa
    14701 tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa tcattgcttt
    14761 atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa aatcaaaaaa
    14821 gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac aaaatgcatt
    14881 tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt cagactattt
    14941 aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg atttaaagat
    15001 caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag gaaaagaagt
    15061 atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg gtttcgaacg
    15121 gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc tccgctgtat
    15181 gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa gtaccacgaa
    15241 ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta gtctcaaaga
    15301 tattagctgg caaagatatc cccagttaaa aatagatttc tgtatccaac aataaaaata
    15361 tttttgtatt tattagtata tatactttac aaaaatttgt atatatacta atttttttgt
    15421 aggatcaatg aaatgagggg aaaatatgcc aaaatataaa gaaatagcct tggctctaaa
    15481 agagaggatc gttgagggaa aatatcagga aggttctaaa ttacctgagc aagaaagtct
    15541 ggctagagag tttaaaacaa gccgtgttac tatccgcaaa gctatacaac tccttattga
    15601 tgaaggtttg ttatatacga gacgtggatc tggtactttt atacgaagca atatcagaca
    15661 ggacaatgag aatgtcactc aagtaaataa tgtatttggc acttcctcac aagaaaatac
    15721 aaatgtgaca agtaaaataa tccgttttga tgtccggttt ccgacagatt atgaaaggcg
    15781 gctttctgca acctacttcc gatccccttc ctgcaaatca tgggtataaa aaataggcat
    15841 attaagcggg ctaggaggag aaatattcac atatcatttt ttcattccgc aagctgcgtc
    15901 aagttatttg gaatttgtag aacagatgag agaggtagaa gcagcactac aaactacttt
    15961 tcaataagta ggaattatag aaattttact caaaaaatct tcattaaaaa atctgactaa
    16021 ataagttgag aaaacagaat atacttcaat atccagtgat ttataaatat cattcactca
    16081 tgatacccag ctccgtatca taagtgatgc ggtttttatc attgatccta aaaatcttag
    16141 caagacaaaa agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac
    16201 aatgataggt tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa
    16261 gcttttgagc taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa
    16321 gtacaggata agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac
    16381 caagtttttg acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat
    16441 aagccgatag tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg
    16501 ctaaaaaaat taaaaatcgc cttgtctatg accactgatg atattttaga agtttttgct
    16561 gaggcagaac tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa
    16621 cgaaatttta agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt
    16681 tataatcgga gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat
    16741 gagagctagt ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt
    16801 atgaatgatg aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga
    16861 tgtaggctca attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta
    16921 gcgaataaat cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt
    16981 agtagaagcg aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg
    17041 ttaaataaga gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata
    17101 attgattcat taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat
    17161 ggaatggtca ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt
    17221 ttttatcaac tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa
    17281 tatggcaatg aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct
    17341 tggataattc cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa
    17401 ctaattaata aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt
    17461 tttcatgata atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt
    17521 aaataagtgt attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc
    17581 aacatcgtac tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga
    17641 aggttttagt atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt
    17701 tctatgaatg tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat
    17761 tctattccag tttttattga tttgctgata aaataacttg aatggtggat ataaacatga
    17821 taaataagag aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga
    17881 aagaaaaagg aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt
    17941 cgctttgggt taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca
    18001 gaacatgtcc aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta
    18061 gaagcaaaac taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg
    18121 attattctat ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta
    18181 atcaaaaaag atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt
    18241 gagaaatttg taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt
    18301 gaaggtcctg gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt
    18361 gatggaaaag aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat
    18421 gctcattttt ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca
    18481 atgaacggac tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact
    18541 gctcataaaa tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag
    18601 attgaactag atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca
    18661 attgggttac attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa
    18721 atcgattata tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct
    18781 tattgtgatt tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa
    18841 aggagagatt gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc
    18901 ctcgctggta cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc
    18961 attttaaaat attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt
    19021 tttcaactag caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat
    19081 ccgatgagaa tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc
    19141 acacctgatt tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat
    19201 acgatgctga cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg
    19261 tcagtgtcga ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt
    19321 tatttattcc cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc
    19381 actaccttac aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt
    19441 atctctccta tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct
    19501 ggtgcggctg cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag
    19561 gttaataagt caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct
    19621 aatctattta gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct
    19681 attcctgtcg ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc
    19741 ggacttgttg gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt
    19801 tgttggttag tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta
    19861 tttaaactgt atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga
    19921 aattttcaat caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg
    19981 cctttttgag taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta
    20041 aaatgtagaa gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa
    20101 tatgtgttaa aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta
    20161 ggagaagaaa atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat
    20221 tagtgattta tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac
    20281 taccgtaatt agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg
    20341 gtcagttgta tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat
    20401 tggaacatca aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg
    20461 tgattgtatt tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc
    20521 gacttggatc cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt
    20581 gttacttgtt ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt
    20641 tttaacagtt attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat
    20701 tagtcagttt gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa
    20761 ctatttgccg atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga
    20821 agctgattaa gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa
    20881 gaatagagat gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa
    20941 tttcaggtag tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt
    21001 tagatgtgga gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag
    21061 aatgggaaag aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc
    21121 aggtgataga aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac
    21181 cagatgcgat ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg
    21241 attatgccaa acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg
    21301 gctgtttctg gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat
    21361 ctggttatac aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata
    21421 ctgggcatgt tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc
    21481 tgttggagat ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc
    21541 gggggaaaca atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg
    21601 aaaaatcaag agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga
    21661 ttcgccaagc aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc
    21721 aacctaaaag atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac
    21781 atagatagag gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga
    21841 caaaaatagc ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga
    21901 acaccgagga aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg
    21961 tatgacaagt cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag
    22021 agaattcgaa ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata
    22081 attaatagac acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc
    22141 aaacaataac ta
//
LOCUS       DAFBYI010000044        21266 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFBYI010000044 DAFBYI010000000
VERSION     DAFBYI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.044"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..158)
                     /gene="nadD"
                     /locus_tag="LLD04_001405"
     CDS             complement(<1..158)
                     /gene="nadD"
                     /locus_tag="LLD04_001405"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="transfers an adenyl group from ATP to NaMN to form
                     nicotinic acid adenine dinucleotide (NaAD) which is then
                     converted to the ubiquitous compound NAD by NAD
                     synthetase; essential enzyme in bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK5648343.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGR"
     gene            complement(168..479)
                     /gene="yhbY"
                     /locus_tag="LLD04_001406"
     CDS             complement(168..479)
                     /gene="yhbY"
                     /locus_tag="LLD04_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK5648344.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(495..1604)
                     /gene="yqeH"
                     /locus_tag="LLD04_001407"
     CDS             complement(495..1604)
                     /gene="yqeH"
                     /locus_tag="LLD04_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK5648345.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(1601..2128)
                     /locus_tag="LLD04_001408"
     CDS             complement(1601..2128)
                     /locus_tag="LLD04_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK5648346.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(2308..3093)
                     /locus_tag="LLD04_001409"
     CDS             complement(2308..3093)
                     /locus_tag="LLD04_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK5648347.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(3086..3955)
                     /locus_tag="LLD04_001410"
     CDS             complement(3086..3955)
                     /locus_tag="LLD04_001410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK5648348.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(3956..5335)
                     /locus_tag="LLD04_001411"
     CDS             complement(3956..5335)
                     /locus_tag="LLD04_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK5648349.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(5343..5762)
                     /gene="fabZ"
                     /locus_tag="LLD04_001412"
     CDS             complement(5343..5762)
                     /gene="fabZ"
                     /locus_tag="LLD04_001412"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5648350.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(5787..6263)
                     /gene="accB"
                     /locus_tag="LLD04_001413"
     CDS             complement(5787..6263)
                     /gene="accB"
                     /locus_tag="LLD04_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK5648351.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(6266..7501)
                     /gene="fabF"
                     /locus_tag="LLD04_001414"
     CDS             complement(6266..7501)
                     /gene="fabF"
                     /locus_tag="LLD04_001414"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK5648352.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(7520..8257)
                     /gene="fabG"
                     /locus_tag="LLD04_001415"
     CDS             complement(7520..8257)
                     /gene="fabG"
                     /locus_tag="LLD04_001415"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK5648353.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(8269..9186)
                     /gene="fabD"
                     /locus_tag="LLD04_001416"
     CDS             complement(8269..9186)
                     /gene="fabD"
                     /locus_tag="LLD04_001416"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5648354.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(9293..9517)
                     /locus_tag="LLD04_001417"
     CDS             complement(9293..9517)
                     /locus_tag="LLD04_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5648355.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(9570..10532)
                     /locus_tag="LLD04_001418"
     CDS             complement(9570..10532)
                     /locus_tag="LLD04_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK5648356.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(10542..10982)
                     /locus_tag="LLD04_001419"
     CDS             complement(10542..10982)
                     /locus_tag="LLD04_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5648357.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            11288..12253
                     /locus_tag="LLD04_001420"
     CDS             11288..12253
                     /locus_tag="LLD04_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5648358.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(12310..12540)
                     /locus_tag="LLD04_001421"
     CDS             complement(12310..12540)
                     /locus_tag="LLD04_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK5648359.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(12626..13513)
                     /locus_tag="LLD04_001422"
     CDS             complement(12626..13513)
                     /locus_tag="LLD04_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK5648360.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(13602..14825)
                     /locus_tag="LLD04_001423"
     CDS             complement(13602..14825)
                     /locus_tag="LLD04_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5648361.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(14842..15888)
                     /locus_tag="LLD04_001424"
     CDS             complement(14842..15888)
                     /locus_tag="LLD04_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5648362.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(16052..17779)
                     /gene="ptsP"
                     /locus_tag="LLD04_001425"
     CDS             complement(16052..17779)
                     /gene="ptsP"
                     /locus_tag="LLD04_001425"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK5648363.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(17779..18045)
                     /locus_tag="LLD04_001426"
     CDS             complement(17779..18045)
                     /locus_tag="LLD04_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK5648364.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            18524..20758
                     /locus_tag="LLD04_001427"
     CDS             18524..20758
                     /locus_tag="LLD04_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5648365.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            20906..21226
                     /locus_tag="LLD04_001428"
     CDS             20906..21226
                     /locus_tag="LLD04_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5648366.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         5947 a         4606 c         3709 g         7004 t
ORIGIN      
        1 ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg
       61 cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat
      121 tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt
      181 acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca
      241 cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct
      301 tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa
      361 gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc
      421 ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata
      481 attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc
      541 cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg
      601 tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca
      661 tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt
      721 tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga
      781 ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc
      841 agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta
      901 cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc
      961 ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg
     1021 attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg
     1081 acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct
     1141 aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc tactggacga
     1201 attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat
     1261 ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg
     1321 tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat
     1381 gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca
     1441 tcttggatct cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa
     1501 gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt
     1561 tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc
     1621 agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat
     1681 taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc
     1741 ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat
     1801 catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg
     1861 ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat
     1921 tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca
     1981 actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata
     2041 ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca
     2101 tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc
     2161 acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat
     2221 actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc
     2281 acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc
     2341 cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt
     2401 cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac
     2461 tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc
     2521 ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac
     2581 agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc
     2641 gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc
     2701 gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata
     2761 tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat
     2821 cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt
     2881 ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg
     2941 agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc
     3001 gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc
     3061 taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca
     3121 attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa
     3181 aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg
     3241 tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa
     3301 tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag
     3361 ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct
     3421 gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga
     3481 ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg
     3541 ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct
     3601 gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt
     3661 taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc
     3721 attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa
     3781 aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat
     3841 gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt
     3901 tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg
     3961 cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg
     4021 tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt
     4081 tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt
     4141 caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga
     4201 tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca
     4261 gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa
     4321 tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct
     4381 caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta
     4441 ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat
     4501 ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag
     4561 cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca
     4621 gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac
     4681 caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca
     4741 aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct
     4801 gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg
     4861 ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag
     4921 ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg
     4981 cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat
     5041 tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat
     5101 gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg
     5161 aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg
     5221 cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac
     5281 gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc
     5341 acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg
     5401 attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag
     5461 gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc
     5521 gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct
     5581 tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc
     5641 tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact
     5701 cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc
     5761 attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca
     5821 tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta
     5881 gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact
     5941 tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc
     6001 acgattggcg aaacaacctc gattccatca aatactgccg attctttttc tttcattatt
     6061 tctggagcag ttgttgcttg ttcagatatg acattgactg gctccgaaac tggcaacgtt
     6121 tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca
     6181 aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat
     6241 tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat
     6301 tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt
     6361 cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg
     6421 ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc
     6481 ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac
     6541 ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt
     6601 gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca
     6661 ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt
     6721 ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta
     6781 aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt
     6841 caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa
     6901 atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag
     6961 aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag
     7021 ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg
     7081 ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat
     7141 gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga
     7201 cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta
     7261 aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa
     7321 aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag
     7381 catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc
     7441 tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca
     7501 tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt
     7561 gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt
     7621 gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa
     7681 cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag
     7741 ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat
     7801 aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta atgatggccc
     7861 cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca
     7921 aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat
     7981 ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt
     8041 tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta
     8101 caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa
     8161 tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac
     8221 tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt atagtattgc
     8281 tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat
     8341 ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc
     8401 gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc
     8461 aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg
     8521 aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg
     8581 ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc
     8641 tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat
     8701 ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca
     8761 gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc
     8821 tggggtagct tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa
     8881 ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc
     8941 atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat
     9001 agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc
     9061 catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc
     9121 atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt
     9181 tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc
     9241 cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag
     9301 tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga
     9361 ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag
     9421 cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt
     9481 cttctgcgat gatttcttga attttttcga ataccataat aatatattcc tccgatattg
     9541 tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt
     9601 aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca
     9661 ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta
     9721 tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata
     9781 attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct
     9841 tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc
     9901 aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca
     9961 gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc
    10021 tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta
    10081 cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt
    10141 gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca
    10201 gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct
    10261 acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta
    10321 gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc
    10381 tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca
    10441 gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg
    10501 gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag
    10561 aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg
    10621 tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt
    10681 ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg
    10741 acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat
    10801 agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg
    10861 tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt
    10921 gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc
    10981 atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct
    11041 taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa
    11101 gattttactc atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa
    11161 tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc
    11221 cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg
    11281 agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat
    11341 gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt
    11401 aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca
    11461 tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg
    11521 tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta
    11581 taattatgta catattacca ataagggatt tgtattaaca gatactccta cggctcgaac
    11641 atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac
    11701 ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga
    11761 cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg
    11821 aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa
    11881 tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg
    11941 gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc
    12001 agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat
    12061 gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc
    12121 tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg
    12181 caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa
    12241 gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta
    12301 tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa
    12361 ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata
    12421 agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc
    12481 tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat
    12541 cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt
    12601 gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc
    12661 accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt
    12721 catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt
    12781 ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt
    12841 cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa
    12901 gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg
    12961 tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt
    13021 tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt
    13081 gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta
    13141 agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg
    13201 caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga
    13261 tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac
    13321 ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga
    13381 tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt
    13441 cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa
    13501 aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat
    13561 acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt
    13621 aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca
    13681 tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa
    13741 atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa
    13801 tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga
    13861 tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc
    13921 gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc
    13981 gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct
    14041 tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg
    14101 ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct
    14161 tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga
    14221 agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat
    14281 ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt
    14341 aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat
    14401 ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg
    14461 tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc
    14521 atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt
    14581 tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa
    14641 gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga
    14701 tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt
    14761 ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt
    14821 ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga
    14881 gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat
    14941 acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt
    15001 aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct
    15061 atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc
    15121 taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc
    15181 tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt
    15241 ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac
    15301 atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat
    15361 gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag
    15421 tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc
    15481 gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt
    15541 tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt
    15601 ttttacgata ggagaaagct ggttcgatcc gataaaggaa tttcggaaat cttcctctgt
    15661 agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg
    15721 actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt
    15781 ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc
    15841 agattttgca agcatctttt cactttcaaa atataataat accttcacct tacgtccccc
    15901 ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg
    15961 tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt
    16021 ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa
    16081 cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg
    16141 ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga
    16201 actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc
    16261 cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac
    16321 gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg
    16381 ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg
    16441 caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg
    16501 tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt
    16561 ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat
    16621 gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag
    16681 ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca
    16741 gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt
    16801 ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg
    16861 gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat
    16921 ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt
    16981 cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat
    17041 ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc
    17101 cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg
    17161 tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc
    17221 caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg
    17281 gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag
    17341 taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct
    17401 cttgcatata agcattatct tccattgctt cgaacatacc gatatacata tccgttactt
    17461 cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta
    17521 ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac
    17581 ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg
    17641 cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta
    17701 aatccggttg aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc
    17761 cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc
    17821 tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc
    17881 tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc
    17941 taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc
    18001 gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct
    18061 ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac
    18121 aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc
    18181 attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac
    18241 attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta
    18301 tcattctttc ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt
    18361 atttttagta taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta
    18421 cctcataaat ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta
    18481 aaatatggat gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg
    18541 cggcaagaat gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact
    18601 cgattactgt cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa
    18661 tatggcccaa aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc
    18721 tcgccaaatg caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa
    18781 caacaacttc aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata
    18841 cggaatcaat atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga
    18901 tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt
    18961 gattggagaa ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt
    19021 agatggtgat gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc
    19081 tttagtccaa ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga
    19141 agagatccgt caagcagaaa atgtgatcct attcatagat gaagttcacg aaatcgtcgg
    19201 tgctggttct gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc
    19261 tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa
    19321 agacgctgct ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga
    19381 aacgatcagt attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata
    19441 tactgatgag gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt
    19501 cttgccggat aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat
    19561 ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt
    19621 tttagcttct cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa
    19681 acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa
    19741 agatatggaa aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa
    19801 agaacagaca caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga
    19861 taacgctgta gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca
    19921 gaatcgtcca atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct
    19981 tgcgaaacaa cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat
    20041 gtctgaatat atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt
    20101 cggatacgaa gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt
    20161 cttgttagat gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt
    20221 agatgatggt cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat
    20281 cattatgaca agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc
    20341 acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt
    20401 tctgaaccgt tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac
    20461 aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat
    20521 cgaagttcct acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg
    20581 tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta
    20641 tcttgatcac ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat
    20701 cgttacttct aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat
    20761 ccagttttat agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga
    20821 aaagtcaaaa tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta
    20881 aaaatacata aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg
    20941 tcttgtatta aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg
    21001 aaataccact attgtctaca ctgaagcccc tgaacataat gaaattctgc tgatcaatga
    21061 caaacgtaaa atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat
    21121 caaagacgcc gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga
    21181 aatctcgatt ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa attttgaaca
    21241 taataattaa agagcgtggg acaaaa
//
LOCUS       DAFBYI010000045        21114 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFBYI010000045 DAFBYI010000000
VERSION     DAFBYI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.045"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            474..725
                     /locus_tag="LLD04_001429"
     CDS             474..725
                     /locus_tag="LLD04_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648367.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="LLD04_001430"
     CDS             759..1037
                     /locus_tag="LLD04_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648368.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="LLD04_001431"
     CDS             1634..1891
                     /locus_tag="LLD04_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648369.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="LLD04_001432"
     CDS             2004..2615
                     /locus_tag="LLD04_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648370.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="LLD04_001433"
     CDS             2794..3351
                     /locus_tag="LLD04_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648371.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="LLD04_001434"
     CDS             complement(4050..4571)
                     /locus_tag="LLD04_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5648372.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="LLD04_001435"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="LLD04_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK5648373.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="LLD04_001436"
     CDS             8651..9538
                     /locus_tag="LLD04_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK5648374.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="LLD04_001437"
     CDS             9535..10704
                     /locus_tag="LLD04_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648375.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="LLD04_001438"
     CDS             10728..12083
                     /locus_tag="LLD04_001438"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK5648376.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LLD04_001439"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LLD04_001439"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK5648377.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="LLD04_001440"
     CDS             14369..14659
                     /locus_tag="LLD04_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648378.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="LLD04_001441"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="LLD04_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK5648379.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="LLD04_001442"
     CDS             15700..16359
                     /locus_tag="LLD04_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5648380.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="LLD04_001443"
     CDS             16516..17448
                     /locus_tag="LLD04_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648381.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="LLD04_001444"
     CDS             17602..18891
                     /locus_tag="LLD04_001444"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK5648382.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="LLD04_001445"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="LLD04_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK5648383.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="LLD04_001446"
     CDS             20471..21058
                     /locus_tag="LLD04_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK5648384.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7025 a         3605 c         4568 g         5916 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagt
//
LOCUS       DAFBYI010000046        20838 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFBYI010000046 DAFBYI010000000
VERSION     DAFBYI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.046"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(39..1241)
                     /locus_tag="LLD04_001447"
     CDS             complement(39..1241)
                     /locus_tag="LLD04_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5648385.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="LLD04_001448"
     CDS             1358..1873
                     /locus_tag="LLD04_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5648386.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="LLD04_001449"
     CDS             complement(2072..2518)
                     /locus_tag="LLD04_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5648387.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr(C)"
                     /locus_tag="LLD04_001450"
     CDS             complement(2565..4043)
                     /gene="msr(C)"
                     /locus_tag="LLD04_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5648388.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="LLD04_001451"
     CDS             complement(4397..5026)
                     /locus_tag="LLD04_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5648389.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="LLD04_001452"
     CDS             complement(5010..5474)
                     /locus_tag="LLD04_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5648390.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="LLD04_001453"
     CDS             complement(5961..7229)
                     /locus_tag="LLD04_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5648391.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="LLD04_001454"
     CDS             complement(7460..8197)
                     /locus_tag="LLD04_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5648392.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="LLD04_001455"
     CDS             complement(8264..8833)
                     /locus_tag="LLD04_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK5648393.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="LLD04_001456"
     CDS             8961..9833
                     /locus_tag="LLD04_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5648394.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="LLD04_001457"
     CDS             10175..11047
                     /locus_tag="LLD04_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5648395.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="LLD04_001458"
     CDS             11044..13002
                     /locus_tag="LLD04_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5648396.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="LLD04_001459"
     CDS             complement(13253..14104)
                     /locus_tag="LLD04_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5648397.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="LLD04_001460"
     CDS             14227..14694
                     /locus_tag="LLD04_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK5648398.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="LLD04_001461"
     CDS             complement(14957..15439)
                     /locus_tag="LLD04_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5648399.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="LLD04_001462"
     CDS             complement(15539..16402)
                     /locus_tag="LLD04_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5648400.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17000..17848
                     /locus_tag="LLD04_001463"
     CDS             17000..17848
                     /locus_tag="LLD04_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648401.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18019..18360)
                     /locus_tag="LLD04_001464"
     CDS             complement(18019..18360)
                     /locus_tag="LLD04_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK5648402.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18446..19153
                     /locus_tag="LLD04_001465"
     CDS             18446..19153
                     /locus_tag="LLD04_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5648403.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19140..19463
                     /locus_tag="LLD04_001466"
     CDS             19140..19463
                     /locus_tag="LLD04_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5648404.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19552..20589)
                     /locus_tag="LLD04_001467"
     CDS             complement(19552..20589)
                     /locus_tag="LLD04_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5648405.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6209 a         4311 c         3699 g         6619 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatatac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    16741 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    16801 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    16861 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    16921 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    16981 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    17041 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    17101 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    17161 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    17221 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    17281 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    17341 tcttatagaa catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    17401 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    17461 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    17521 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    17581 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    17641 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    17701 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    17761 ctctataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    17821 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    17881 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    17941 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    18001 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
    18061 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc
    18121 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
    18181 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac
    18241 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
    18301 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
    18361 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
    18421 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
    18481 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
    18541 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
    18601 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
    18661 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
    18721 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
    18781 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
    18841 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
    18901 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
    18961 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
    19021 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
    19081 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
    19141 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
    19201 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
    19261 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
    19321 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
    19381 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
    19441 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
    19501 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
    19561 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
    19621 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
    19681 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
    19741 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
    19801 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
    19861 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
    19921 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
    19981 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
    20041 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
    20101 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
    20161 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
    20221 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
    20281 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
    20341 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
    20401 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
    20461 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
    20521 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
    20581 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
    20641 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
    20701 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
    20761 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattcatc
    20821 aaacatgaga agctgttt
//
LOCUS       DAFBYI010000047        20609 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFBYI010000047 DAFBYI010000000
VERSION     DAFBYI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.047"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="LLD04_001468"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="LLD04_001468"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK5648406.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="LLD04_001469"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="LLD04_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK5648407.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="LLD04_001470"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="LLD04_001470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK5648408.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="LLD04_001471"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="LLD04_001471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK5648409.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="LLD04_001472"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="LLD04_001472"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK5648410.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="LLD04_001473"
     CDS             5265..5843
                     /locus_tag="LLD04_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5648411.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="LLD04_001474"
     CDS             5895..6377
                     /locus_tag="LLD04_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK5648412.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="LLD04_001475"
     CDS             6494..6868
                     /locus_tag="LLD04_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648413.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="LLD04_001476"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="LLD04_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5648414.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="LLD04_001477"
     CDS             9167..9757
                     /locus_tag="LLD04_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5648415.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="LLD04_001478"
     CDS             complement(9885..11234)
                     /locus_tag="LLD04_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5648416.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="LLD04_001479"
     CDS             complement(11402..12142)
                     /locus_tag="LLD04_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5648417.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="LLD04_001480"
     CDS             complement(12155..13747)
                     /locus_tag="LLD04_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5648418.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="LLD04_001481"
     CDS             complement(14315..14611)
                     /locus_tag="LLD04_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648419.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="LLD04_001482"
     CDS             14787..15512
                     /locus_tag="LLD04_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK5648420.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="LLD04_001483"
     CDS             15505..16347
                     /locus_tag="LLD04_001483"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK5648421.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="LLD04_001484"
     CDS             16350..16871
                     /locus_tag="LLD04_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK5648422.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="LLD04_001485"
     CDS             complement(17124..17687)
                     /locus_tag="LLD04_001485"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5648423.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="LLD04_001486"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="LLD04_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK5648424.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="LLD04_001487"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="LLD04_001487"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK5648425.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT         6737 a         3959 c         4165 g         5748 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccg
//
LOCUS       DAFBYI010000048        19411 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFBYI010000048 DAFBYI010000000
VERSION     DAFBYI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.048"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            168..362
                     /locus_tag="LLD04_001488"
     CDS             168..362
                     /locus_tag="LLD04_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648426.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LLD04_001489"
     CDS             631..930
                     /locus_tag="LLD04_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648427.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LLD04_001490"
     CDS             complement(1027..1461)
                     /locus_tag="LLD04_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5648428.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LLD04_001491"
     CDS             complement(1693..2403)
                     /locus_tag="LLD04_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5648429.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LLD04_001492"
     CDS             complement(2393..3253)
                     /locus_tag="LLD04_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK5648430.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LLD04_001493"
     CDS             complement(3258..3896)
                     /locus_tag="LLD04_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK5648431.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LLD04_001494"
     CDS             complement(3911..4210)
                     /locus_tag="LLD04_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5648432.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LLD04_001495"
     CDS             complement(4253..5737)
                     /locus_tag="LLD04_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5648433.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIKKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LLD04_001496"
     CDS             complement(5757..6218)
                     /locus_tag="LLD04_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648434.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LLD04_001497"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LLD04_001497"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK5648435.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LLD04_001498"
     CDS             7521..8291
                     /locus_tag="LLD04_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5648436.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LLD04_001499"
     CDS             complement(8564..9391)
                     /locus_tag="LLD04_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648437.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVVMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LLD04_001500"
     CDS             9959..11194
                     /locus_tag="LLD04_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5648438.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LLD04_001501"
     CDS             complement(12193..12453)
                     /locus_tag="LLD04_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5648439.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LLD04_001502"
     CDS             complement(12480..13781)
                     /locus_tag="LLD04_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5648440.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LLD04_001503"
     CDS             complement(14073..14531)
                     /locus_tag="LLD04_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5648441.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LLD04_001504"
     CDS             complement(14544..15875)
                     /locus_tag="LLD04_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK5648442.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LLD04_001505"
     CDS             complement(15886..17328)
                     /locus_tag="LLD04_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5648443.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LLD04_001506"
     CDS             complement(17315..18079)
                     /locus_tag="LLD04_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK5648444.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LLD04_001507"
     CDS             complement(18183..19058)
                     /locus_tag="LLD04_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5648445.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="LLD04_001508"
     tRNA            complement(19313..19397)
                     /locus_tag="LLD04_001508"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
BASE COUNT         6178 a         3990 c         2985 g         6258 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcttgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattacc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata a
//
LOCUS       DAFBYI010000049        18895 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFBYI010000049 DAFBYI010000000
VERSION     DAFBYI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.049"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(21..251)
                     /locus_tag="LLD04_001509"
     CDS             complement(21..251)
                     /locus_tag="LLD04_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648446.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LLD04_001510"
     CDS             complement(687..1253)
                     /locus_tag="LLD04_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5648447.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LLD04_001511"
     CDS             complement(1253..2209)
                     /locus_tag="LLD04_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK5648448.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LLD04_001512"
     CDS             complement(2209..2865)
                     /locus_tag="LLD04_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5648449.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LLD04_001513"
     CDS             3235..5253
                     /locus_tag="LLD04_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5648450.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LLD04_001514"
     CDS             5680..8094
                     /locus_tag="LLD04_001514"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK5648451.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LLD04_001515"
     CDS             8442..9995
                     /locus_tag="LLD04_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK5648452.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LLD04_001516"
     CDS             complement(10376..12160)
                     /locus_tag="LLD04_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648453.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LLD04_001517"
     CDS             complement(12175..13896)
                     /locus_tag="LLD04_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648454.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LLD04_001518"
     CDS             13996..14757
                     /locus_tag="LLD04_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5648455.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="LLD04_001519"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="LLD04_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="LLD04_001520"
     CDS             16664..>18895
                     /locus_tag="LLD04_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648456.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DAFBYI010000050        18823 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFBYI010000050 DAFBYI010000000
VERSION     DAFBYI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.050"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            83..970
                     /locus_tag="LLD04_001521"
     CDS             83..970
                     /locus_tag="LLD04_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5648457.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="LLD04_001522"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="LLD04_001522"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK5648458.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="LLD04_001523"
     CDS             2272..2718
                     /locus_tag="LLD04_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5648459.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="LLD04_001524"
     CDS             2991..4343
                     /locus_tag="LLD04_001524"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK5648460.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="LLD04_001525"
     CDS             4514..4801
                     /locus_tag="LLD04_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648461.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="LLD04_001526"
     CDS             5027..5470
                     /locus_tag="LLD04_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5648462.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="LLD04_001527"
     CDS             complement(5476..6660)
                     /locus_tag="LLD04_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK5648463.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="LLD04_001528"
     CDS             6928..7626
                     /locus_tag="LLD04_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5648464.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="LLD04_001529"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="LLD04_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK5648465.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="LLD04_001530"
     CDS             complement(8436..8969)
                     /locus_tag="LLD04_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648466.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="LLD04_001531"
     CDS             complement(9266..11647)
                     /locus_tag="LLD04_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5648467.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LLD04_001532"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LLD04_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK5648468.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="LLD04_001533"
     CDS             12348..12887
                     /locus_tag="LLD04_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK5648469.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="LLD04_001534"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="LLD04_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK5648470.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="LLD04_001535"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LLD04_001535"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="LLD04_001536"
     CDS             13994..15160
                     /locus_tag="LLD04_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK5648471.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="LLD04_001537"
     CDS             15206..16702
                     /locus_tag="LLD04_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK5648472.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="LLD04_001538"
     CDS             16783..17466
                     /locus_tag="LLD04_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5648473.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..>18823
                     /locus_tag="LLD04_001539"
     CDS             18111..>18823
                     /locus_tag="LLD04_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK5648474.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDM"
BASE COUNT         6404 a         3210 c         3895 g         5314 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gca
//
LOCUS       DAFBYI010000051        18539 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFBYI010000051 DAFBYI010000000
VERSION     DAFBYI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.051"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            259..744
                     /locus_tag="LLD04_001540"
     CDS             259..744
                     /locus_tag="LLD04_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK5648475.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            737..1420
                     /locus_tag="LLD04_001541"
     CDS             737..1420
                     /locus_tag="LLD04_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK5648476.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1546..4191
                     /gene="polA"
                     /locus_tag="LLD04_001542"
     CDS             1546..4191
                     /gene="polA"
                     /locus_tag="LLD04_001542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK5648477.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4236..5072
                     /gene="mutM"
                     /locus_tag="LLD04_001543"
     CDS             4236..5072
                     /gene="mutM"
                     /locus_tag="LLD04_001543"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK5648478.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5036..5665
                     /locus_tag="LLD04_001544"
     CDS             5036..5665
                     /locus_tag="LLD04_001544"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK5648479.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5717..6049
                     /locus_tag="LLD04_001545"
     CDS             5717..6049
                     /locus_tag="LLD04_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5648480.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6361..6852
                     /gene="nrdR"
                     /locus_tag="LLD04_001546"
     CDS             6361..6852
                     /gene="nrdR"
                     /locus_tag="LLD04_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK5648481.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6875..8248
                     /locus_tag="LLD04_001547"
     CDS             6875..8248
                     /locus_tag="LLD04_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5648482.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8248..9174
                     /gene="dnaI"
                     /locus_tag="LLD04_001548"
     CDS             8248..9174
                     /gene="dnaI"
                     /locus_tag="LLD04_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK5648483.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9303..9743
                     /locus_tag="LLD04_001549"
     CDS             9303..9743
                     /locus_tag="LLD04_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK5648484.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9887..10588
                     /locus_tag="LLD04_001550"
     CDS             9887..10588
                     /locus_tag="LLD04_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK5648485.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10585..11706
                     /locus_tag="LLD04_001551"
     CDS             10585..11706
                     /locus_tag="LLD04_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK5648486.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11721..12953
                     /gene="pepT"
                     /locus_tag="LLD04_001552"
     CDS             11721..12953
                     /gene="pepT"
                     /locus_tag="LLD04_001552"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK5648487.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13085..13993
                     /locus_tag="LLD04_001553"
     CDS             13085..13993
                     /locus_tag="LLD04_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK5648488.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            14028..14300
                     /locus_tag="LLD04_001554"
     CDS             14028..14300
                     /locus_tag="LLD04_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK5648489.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14311..15357
                     /locus_tag="LLD04_001555"
     CDS             14311..15357
                     /locus_tag="LLD04_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK5648490.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15608..18217
                     /gene="clpB"
                     /locus_tag="LLD04_001556"
     CDS             15608..18217
                     /gene="clpB"
                     /locus_tag="LLD04_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK5648491.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18368..>18539)
                     /locus_tag="LLD04_001557"
     CDS             complement(18368..>18539)
                     /locus_tag="LLD04_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648492.1"
                     /translation="ASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERY
                     FESYKLEKVKNT"
BASE COUNT         6293 a         3242 c         4041 g         4963 t
ORIGIN      
        1 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
       61 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
      121 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
      181 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
      241 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
      301 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
      361 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
      421 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
      481 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
      541 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
      601 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
      661 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
      721 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
      781 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
      841 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
      901 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
      961 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     1021 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     1081 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     1141 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     1201 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     1261 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     1321 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     1381 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     1441 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     1501 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     1561 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     1621 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     1681 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     1741 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     1801 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     1861 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     1921 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     1981 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     2041 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     2101 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     2161 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     2221 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     2281 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     2341 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     2401 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     2461 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     2521 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     2581 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     2641 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     2701 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     2761 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     2821 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     2881 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     2941 ttgagccaag aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca
     3001 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
     3061 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
     3121 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
     3181 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
     3241 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
     3301 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
     3361 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
     3421 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
     3481 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
     3541 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
     3601 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
     3661 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
     3721 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
     3781 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
     3841 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
     3901 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
     3961 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
     4021 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
     4081 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
     4141 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
     4201 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
     4261 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
     4321 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
     4381 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
     4441 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
     4501 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
     4561 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
     4621 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
     4681 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
     4741 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
     4801 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
     4861 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
     4921 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
     4981 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
     5041 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
     5101 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
     5161 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
     5221 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
     5281 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
     5341 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
     5401 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
     5461 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
     5521 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
     5581 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
     5641 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
     5701 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
     5761 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
     5821 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
     5881 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
     5941 acctgatcgt agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat
     6001 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
     6061 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
     6121 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
     6181 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
     6241 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
     6301 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
     6361 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
     6421 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
     6481 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
     6541 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
     6601 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
     6661 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
     6721 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
     6781 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
     6841 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
     6901 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
     6961 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
     7021 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
     7081 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
     7141 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
     7201 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
     7261 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
     7321 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
     7381 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
     7441 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
     7501 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
     7561 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
     7621 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
     7681 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
     7741 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
     7801 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
     7861 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
     7921 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
     7981 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
     8041 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
     8101 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
     8161 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
     8221 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
     8281 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
     8341 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
     8401 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
     8461 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
     8521 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
     8581 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
     8641 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
     8701 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
     8761 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
     8821 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
     8881 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
     8941 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
     9001 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
     9061 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
     9121 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
     9181 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
     9241 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
     9301 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
     9361 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
     9421 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatta
     9481 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
     9541 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
     9601 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
     9661 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
     9721 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
     9781 tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc
     9841 ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga
     9901 tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga
     9961 cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata
    10021 tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa
    10081 agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc
    10141 acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat
    10201 cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc
    10261 aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc
    10321 agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca
    10381 aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa
    10441 aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga
    10501 acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca
    10561 aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt
    10621 gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga
    10681 acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc
    10741 gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt
    10801 cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa
    10861 catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat
    10921 gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag
    10981 attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa
    11041 gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg
    11101 attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg
    11161 aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa
    11221 caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc
    11281 gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa
    11341 atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga
    11401 atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc
    11461 ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat
    11521 atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt
    11581 cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa
    11641 gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt
    11701 aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca
    11761 aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg
    11821 cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata
    11881 atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt
    11941 ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac
    12001 aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata
    12061 cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa
    12121 cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg
    12181 caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg
    12241 gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag
    12301 attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg
    12361 ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata
    12421 caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag
    12481 ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc
    12541 ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac
    12601 gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa
    12661 tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga
    12721 gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac
    12781 cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc
    12841 tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg
    12901 aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa
    12961 aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta
    13021 aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta
    13081 aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa
    13141 attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact
    13201 agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga
    13261 agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt
    13321 ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct
    13381 tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg
    13441 caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac
    13501 aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg
    13561 ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt
    13621 ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat
    13681 gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa
    13741 atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag
    13801 aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat
    13861 gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga
    13921 tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga
    13981 agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt
    14041 tacacaatct tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg
    14101 ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat
    14161 ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata
    14221 aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc
    14281 tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc
    14341 attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa
    14401 tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct
    14461 gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa
    14521 cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt
    14581 taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa
    14641 aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa
    14701 ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct
    14761 aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt
    14821 gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg
    14881 aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc
    14941 tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt
    15001 tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt
    15061 gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg
    15121 ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta
    15181 ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc
    15241 agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga
    15301 gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc
    15361 ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt
    15421 acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata
    15481 taagtcgaga aaaaaagtca aaaaaccatg tgtatatttt gaccaaaatt gaccaaagcg
    15541 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
    15601 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
    15661 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
    15721 tttacaacct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
    15781 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
    15841 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
    15901 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
    15961 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
    16021 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
    16081 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
    16141 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
    16201 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
    16261 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
    16321 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
    16381 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
    16441 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
    16501 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
    16561 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
    16621 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
    16681 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
    16741 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
    16801 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
    16861 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
    16921 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
    16981 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
    17041 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
    17101 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
    17161 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
    17221 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
    17281 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
    17341 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
    17401 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
    17461 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
    17521 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
    17581 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
    17641 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
    17701 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
    17761 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
    17821 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
    17881 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
    17941 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
    18001 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
    18061 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
    18121 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
    18181 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
    18241 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
    18301 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
    18361 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
    18421 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
    18481 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagca
//
LOCUS       DAFBYI010000052        18185 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFBYI010000052 DAFBYI010000000
VERSION     DAFBYI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.052"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..950
                     /gene="tuf"
                     /locus_tag="LLD04_001558"
     CDS             <1..950
                     /gene="tuf"
                     /locus_tag="LLD04_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5648493.1"
                     /translation="DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSR
                     QVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTEYEFPGDDVPVVAGSALKALEGD
                     ASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDVFSITGRGTVATGRVERGQVRVG
                     DEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPG
                     TITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVELPEGTEMVMPGD
                     NVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTEIKA"
     gene            1357..1665
                     /gene="rpsJ"
                     /locus_tag="LLD04_001559"
     CDS             1357..1665
                     /gene="rpsJ"
                     /locus_tag="LLD04_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK5648494.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            1696..2325
                     /gene="rplC"
                     /locus_tag="LLD04_001560"
     CDS             1696..2325
                     /gene="rplC"
                     /locus_tag="LLD04_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK5648495.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            2353..2976
                     /gene="rplD"
                     /locus_tag="LLD04_001561"
     CDS             2353..2976
                     /gene="rplD"
                     /locus_tag="LLD04_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK5648496.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            2976..3266
                     /gene="rplW"
                     /locus_tag="LLD04_001562"
     CDS             2976..3266
                     /gene="rplW"
                     /locus_tag="LLD04_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK5648497.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            3305..4138
                     /gene="rplB"
                     /locus_tag="LLD04_001563"
     CDS             3305..4138
                     /gene="rplB"
                     /locus_tag="LLD04_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK5648498.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            4181..4459
                     /gene="rpsS"
                     /locus_tag="LLD04_001564"
     CDS             4181..4459
                     /gene="rpsS"
                     /locus_tag="LLD04_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK5648499.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            4482..4829
                     /gene="rplV"
                     /locus_tag="LLD04_001565"
     CDS             4482..4829
                     /gene="rplV"
                     /locus_tag="LLD04_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK5648500.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            4843..5499
                     /gene="rpsC"
                     /locus_tag="LLD04_001566"
     CDS             4843..5499
                     /gene="rpsC"
                     /locus_tag="LLD04_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK5648501.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            5502..5936
                     /gene="rplP"
                     /locus_tag="LLD04_001567"
     CDS             5502..5936
                     /gene="rplP"
                     /locus_tag="LLD04_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK5648502.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            5926..6114
                     /gene="rpmC"
                     /locus_tag="LLD04_001568"
     CDS             5926..6114
                     /gene="rpmC"
                     /locus_tag="LLD04_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK5648503.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            6139..6405
                     /gene="rpsQ"
                     /locus_tag="LLD04_001569"
     CDS             6139..6405
                     /gene="rpsQ"
                     /locus_tag="LLD04_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK5648504.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            6463..6831
                     /gene="rplN"
                     /locus_tag="LLD04_001570"
     CDS             6463..6831
                     /gene="rplN"
                     /locus_tag="LLD04_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK5648505.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            6868..7176
                     /gene="rplX"
                     /locus_tag="LLD04_001571"
     CDS             6868..7176
                     /gene="rplX"
                     /locus_tag="LLD04_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK5648506.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            7204..7743
                     /gene="rplE"
                     /locus_tag="LLD04_001572"
     CDS             7204..7743
                     /gene="rplE"
                     /locus_tag="LLD04_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK5648507.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            7762..7947
                     /locus_tag="LLD04_001573"
     CDS             7762..7947
                     /locus_tag="LLD04_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK5648508.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            7983..8381
                     /gene="rpsH"
                     /locus_tag="LLD04_001574"
     CDS             7983..8381
                     /gene="rpsH"
                     /locus_tag="LLD04_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK5648509.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            8413..8949
                     /gene="rplF"
                     /locus_tag="LLD04_001575"
     CDS             8413..8949
                     /gene="rplF"
                     /locus_tag="LLD04_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK5648510.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            9110..9466
                     /gene="rplR"
                     /locus_tag="LLD04_001576"
     CDS             9110..9466
                     /gene="rplR"
                     /locus_tag="LLD04_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK5648511.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            9487..9987
                     /gene="rpsE"
                     /locus_tag="LLD04_001577"
     CDS             9487..9987
                     /gene="rpsE"
                     /locus_tag="LLD04_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK5648512.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            10002..10181
                     /gene="rpmD"
                     /locus_tag="LLD04_001578"
     CDS             10002..10181
                     /gene="rpmD"
                     /locus_tag="LLD04_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK5648513.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            10225..10665
                     /gene="rplO"
                     /locus_tag="LLD04_001579"
     CDS             10225..10665
                     /gene="rplO"
                     /locus_tag="LLD04_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK5648514.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            10665..11960
                     /gene="secY"
                     /locus_tag="LLD04_001580"
     CDS             10665..11960
                     /gene="secY"
                     /locus_tag="LLD04_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK5648515.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            12022..12669
                     /locus_tag="LLD04_001581"
     CDS             12022..12669
                     /locus_tag="LLD04_001581"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK5648516.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            12860..13078
                     /gene="infA"
                     /locus_tag="LLD04_001582"
     CDS             12860..13078
                     /gene="infA"
                     /locus_tag="LLD04_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK5648517.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            13111..13227
                     /gene="rpmJ"
                     /locus_tag="LLD04_001583"
     CDS             13111..13227
                     /gene="rpmJ"
                     /locus_tag="LLD04_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK5648518.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            13245..13610
                     /gene="rpsM"
                     /locus_tag="LLD04_001584"
     CDS             13245..13610
                     /gene="rpsM"
                     /locus_tag="LLD04_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK5648519.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            13638..14027
                     /gene="rpsK"
                     /locus_tag="LLD04_001585"
     CDS             13638..14027
                     /gene="rpsK"
                     /locus_tag="LLD04_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK5648520.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            14107..15045
                     /locus_tag="LLD04_001586"
     CDS             14107..15045
                     /locus_tag="LLD04_001586"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK5648521.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            15073..15453
                     /gene="rplQ"
                     /locus_tag="LLD04_001587"
     CDS             15073..15453
                     /gene="rplQ"
                     /locus_tag="LLD04_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK5648522.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            15725..16564
                     /locus_tag="LLD04_001588"
     CDS             15725..16564
                     /locus_tag="LLD04_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5648523.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            16540..17409
                     /locus_tag="LLD04_001589"
     CDS             16540..17409
                     /locus_tag="LLD04_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5648524.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            17399..>18185
                     /locus_tag="LLD04_001590"
     CDS             17399..>18185
                     /locus_tag="LLD04_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK5648525.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIF"
BASE COUNT         6030 a         3308 c         3999 g         4848 t
ORIGIN      
        1 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
       61 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
      121 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
      181 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
      241 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
      301 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
      361 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
      421 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
      481 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
      541 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
      601 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
      661 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
      721 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
      781 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
      841 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
      901 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
      961 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
     1021 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata
     1081 gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg
     1141 ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg
     1201 tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag
     1261 gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta
     1321 tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc
     1381 cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact
     1441 gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt
     1501 tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt
     1561 acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg
     1621 aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat
     1681 aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc
     1741 ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta
     1801 ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat
     1861 atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct
     1921 cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa
     1981 gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa
     2041 ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac
     2101 ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt
     2161 aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa
     2221 gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct
     2281 aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg
     2341 aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa
     2401 atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca
     2461 atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct
     2521 gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga
     2581 tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct
     2641 tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa
     2701 aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca
     2761 aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta
     2821 gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta
     2881 acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa
     2941 actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac
     3001 gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag
     3061 tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta
     3121 aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg
     3181 caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa
     3241 tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa
     3301 aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc
     3361 tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa
     3421 ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa
     3481 acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca
     3541 aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg
     3601 agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg
     3661 tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac
     3721 tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg
     3781 tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg
     3841 tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca
     3901 acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac
     3961 tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc
     4021 tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac
     4081 tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt
     4141 actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa
     4201 aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa
     4261 gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata
     4321 taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt
     4381 aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga
     4441 taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat
     4501 cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg
     4561 accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca
     4621 aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact
     4681 ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa
     4741 tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc
     4801 atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat
     4861 ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa
     4921 gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt
     4981 gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca
     5041 attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta
     5101 agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa
     5161 aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt
     5221 gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa
     5281 gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga
     5341 tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa
     5401 gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt
     5461 cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg
     5521 taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag
     5581 tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa
     5641 tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta
     5701 aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta
     5761 aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg
     5821 caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa
     5881 tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa
     5941 atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg
     6001 tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa
     6061 gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg
     6121 aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc
     6181 gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac
     6241 cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac
     6301 acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa
     6361 cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc
     6421 ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt
     6481 cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt
     6541 ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca
     6601 acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa
     6661 tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt
     6721 attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta
     6781 cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac
     6841 acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt
     6901 atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat
     6961 aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct
     7021 cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt
     7081 gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt
     7141 gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac
     7201 tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa
     7261 tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt
     7321 gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg
     7381 atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta
     7441 cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc
     7501 ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa
     7561 aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca
     7621 gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat tgtaacaaca
     7681 gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa
     7741 taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct
     7801 gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt
     7861 tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt
     7921 cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat
     7981 caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg
     8041 ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct
     8101 tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga
     8161 tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta
     8221 tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg
     8281 gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg
     8341 aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg
     8401 aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca
     8461 gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact
     8521 ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac
     8581 gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt
     8641 gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca
     8701 caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca
     8761 ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg
     8821 aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct
     8881 tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact
     8941 ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct
     9001 gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg
     9061 tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa
     9121 accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg
     9181 tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat
     9241 tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc
     9301 aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc
     9361 tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt
     9421 gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata
     9481 ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt
     9541 aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt
     9601 gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa
     9661 gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt
     9721 tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct
     9781 gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct
     9841 ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc
     9901 gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc
     9961 aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta
    10021 ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag
    10081 gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga
    10141 tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta
    10201 gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca
    10261 cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga
    10321 cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg
    10381 ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa
    10441 gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact
    10501 cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta
    10561 gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct
    10621 caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa
    10681 aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt
    10741 ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa
    10801 acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa
    10861 acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc
    10921 ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc
    10981 gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg
    11041 tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg
    11101 tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc
    11161 taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag
    11221 gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata
    11281 ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct
    11341 tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat
    11401 atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag
    11461 ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac
    11521 aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta
    11581 actataatac agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct
    11641 tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa
    11701 gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga
    11761 tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg
    11821 ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg
    11881 ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat
    11941 atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca
    12001 catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa
    12061 ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat
    12121 atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg
    12181 gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct
    12241 gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca
    12301 aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc
    12361 catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt
    12421 ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc
    12481 ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg
    12541 gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta
    12601 aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt
    12661 gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca
    12721 ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt
    12781 gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa
    12841 gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt
    12901 cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc
    12961 tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac
    13021 ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt
    13081 gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg
    13141 tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat
    13201 ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag
    13261 gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg
    13321 gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc
    13381 gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg
    13441 aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt
    13501 accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg
    13561 cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg
    13621 aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga
    13681 atatagaagc aggtattgca catatccatt ctacattcaa taacactatc atcatgatca
    13741 ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa
    13801 gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa
    13861 tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag
    13921 cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc
    13981 cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca
    14041 ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag
    14101 ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat
    14161 ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta
    14221 cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt
    14281 gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg
    14341 aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat
    14401 attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt
    14461 cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta
    14521 acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca
    14581 attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt
    14641 gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca
    14701 gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat
    14761 ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa
    14821 gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt
    14881 cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa
    14941 tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa
    15001 gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg
    15061 agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg
    15121 ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt
    15181 gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg
    15241 catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa
    15301 aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt
    15361 tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat
    15421 ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag
    15481 atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg
    15541 ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct
    15601 tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat
    15661 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    15721 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    15781 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    15841 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    15901 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    15961 ccgacagatg gttggaatgg tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    16021 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    16081 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    16141 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    16201 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    16261 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    16321 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    16381 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    16441 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    16501 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    16561 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    16621 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    16681 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    16741 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    16801 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    16861 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    16921 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    16981 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    17041 gtattggatg aaccaacggc aggacttgat ccccagggta gaaaagaaat gatggagatg
    17101 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    17161 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    17221 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    17281 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    17341 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    17401 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    17461 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    17521 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    17581 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    17641 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    17701 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    17761 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    17821 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    17881 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    17941 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    18001 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    18061 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    18121 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    18181 ctttt
//
LOCUS       DAFBYI010000053        18124 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFBYI010000053 DAFBYI010000000
VERSION     DAFBYI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.053"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(24..722)
                     /locus_tag="LLD04_001591"
     CDS             complement(24..722)
                     /locus_tag="LLD04_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK5648526.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LLD04_001592"
     CDS             complement(715..2067)
                     /locus_tag="LLD04_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK5648527.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LLD04_001593"
     CDS             complement(2290..3690)
                     /locus_tag="LLD04_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK5648528.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LLD04_001594"
     CDS             3851..4588
                     /locus_tag="LLD04_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK5648529.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LLD04_001595"
     CDS             4713..5192
                     /locus_tag="LLD04_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK5648530.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LLD04_001596"
     CDS             5189..5956
                     /locus_tag="LLD04_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648531.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LLD04_001597"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LLD04_001597"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5648532.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LLD04_001598"
     CDS             complement(6859..7461)
                     /locus_tag="LLD04_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5648533.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LLD04_001599"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LLD04_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LLD04_001600"
     CDS             8142..8726
                     /locus_tag="LLD04_001600"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK5648534.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LLD04_001601"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LLD04_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK5648535.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LLD04_001602"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LLD04_001602"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK5648536.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LLD04_001603"
     CDS             10627..10806
                     /locus_tag="LLD04_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648537.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LLD04_001604"
     CDS             10828..11853
                     /locus_tag="LLD04_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK5648538.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LLD04_001605"
     CDS             12028..13272
                     /locus_tag="LLD04_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK5648539.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LLD04_001606"
     CDS             13425..14372
                     /locus_tag="LLD04_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5648540.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LLD04_001607"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LLD04_001607"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK5648541.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LLD04_001608"
     CDS             complement(15274..15516)
                     /locus_tag="LLD04_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648542.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LLD04_001609"
     tRNA            15637..15709
                     /locus_tag="LLD04_001609"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LLD04_001610"
     CDS             complement(15828..16466)
                     /locus_tag="LLD04_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5648543.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LLD04_001611"
     CDS             complement(16589..17221)
                     /locus_tag="LLD04_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5648544.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LLD04_001612"
     CDS             17572..17871
                     /locus_tag="LLD04_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5648545.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6216 a         3161 c         3570 g         5177 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatacg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tata
//
LOCUS       DAFBYI010000054        18014 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFBYI010000054 DAFBYI010000000
VERSION     DAFBYI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.054"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            233..475
                     /locus_tag="LLD04_001613"
     CDS             233..475
                     /locus_tag="LLD04_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBK5648546.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="LLD04_001614"
     CDS             472..942
                     /locus_tag="LLD04_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5648547.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="LLD04_001615"
     CDS             1070..2284
                     /locus_tag="LLD04_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK5648548.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3273..3482
                     /locus_tag="LLD04_001616"
     CDS             3273..3482
                     /locus_tag="LLD04_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBK5648549.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5208
                     /locus_tag="LLD04_001617"
                     /pseudo
     CDS             3594..5208
                     /locus_tag="LLD04_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            5243..6454
                     /locus_tag="LLD04_001618"
                     /pseudo
     CDS             5243..6454
                     /locus_tag="LLD04_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6477..7403
                     /gene="glsA"
                     /locus_tag="LLD04_001619"
     CDS             6477..7403
                     /gene="glsA"
                     /locus_tag="LLD04_001619"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK5648550.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7611..9071
                     /locus_tag="LLD04_001620"
     CDS             7611..9071
                     /locus_tag="LLD04_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648551.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9292..11055
                     /locus_tag="LLD04_001621"
     CDS             9292..11055
                     /locus_tag="LLD04_001621"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK5648552.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAKPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11139..11597
                     /locus_tag="LLD04_001622"
     CDS             11139..11597
                     /locus_tag="LLD04_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK5648553.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11706..12389
                     /locus_tag="LLD04_001623"
     CDS             11706..12389
                     /locus_tag="LLD04_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5648554.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12536..13150
                     /locus_tag="LLD04_001624"
     CDS             12536..13150
                     /locus_tag="LLD04_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK5648555.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13166..13372
                     /locus_tag="LLD04_001625"
     CDS             13166..13372
                     /locus_tag="LLD04_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648556.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13913..15043
                     /locus_tag="LLD04_001626"
     CDS             13913..15043
                     /locus_tag="LLD04_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5648557.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15135..15890
                     /locus_tag="LLD04_001627"
     CDS             15135..15890
                     /locus_tag="LLD04_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5648558.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15948..17990
                     /locus_tag="LLD04_001628"
     CDS             15948..17990
                     /locus_tag="LLD04_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK5648559.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGGL
                     KWQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIF
                     GEDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLV
                     YPKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         6201 a         2898 c         3780 g         5135 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag cccgtattgg aacgcttgca gtattgggct
     4741 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     4801 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     4861 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     4921 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     4981 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5041 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5101 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5161 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5221 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5281 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5341 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5401 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5461 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5521 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5581 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5641 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5701 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     5761 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     5821 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     5881 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     5941 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6001 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6061 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6121 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6181 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6241 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6301 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6361 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6421 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6481 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6541 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6601 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6661 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6721 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     6781 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     6841 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     6901 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     6961 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7021 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7081 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7141 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7201 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7261 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7321 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7381 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7441 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7501 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7561 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7621 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7681 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7741 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     7801 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     7861 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     7921 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     7981 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8041 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8101 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8161 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8221 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8281 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8341 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8401 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8461 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8521 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8581 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8641 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8701 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     8761 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     8821 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     8881 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     8941 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9001 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9061 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9121 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9181 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9241 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9301 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9361 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9421 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9481 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9541 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9601 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9661 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9721 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     9781 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     9841 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     9901 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     9961 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10021 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10081 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10141 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10201 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10261 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10321 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10381 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10441 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10501 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10561 ctagattggg cgttcgaaca aacagcgaag cctgtagcaa tccgaattcc tacacatggt
    10621 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10681 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10741 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    10801 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    10861 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    10921 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    10981 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11041 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11101 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11161 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11221 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11281 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11341 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11401 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11461 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11521 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11581 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11641 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11701 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    11761 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    11821 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    11881 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    11941 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12001 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12061 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12121 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12181 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12241 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12301 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12361 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12421 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12481 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12541 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12601 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12661 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12721 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    12781 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    12841 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    12901 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    12961 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13021 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13081 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13141 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13201 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13261 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13321 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
    13381 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
    13441 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
    13501 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    13561 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    13621 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    13681 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    13741 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    13801 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    13861 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ttttgggtcc
    13921 ttatttgact ttgtccatga tcggtctttt agaagtatac agtgccagtt cttatcgttt
    13981 attgcaagcc gatgaaaata caaaaagtct attattgcgt caactaattt tcatattttt
    14041 gagttggagc gtgatcttct tagctcgttc agtcaaacta cactatttac ttcaccctaa
    14101 aatagcagga tacggtttag ccttatcgat tttcttttta gtattagtaa gaatagggat
    14161 attcggtgtc actgtcaacg gcgcacaacg ttggatctct ctgtttggca ttcaattcca
    14221 gccttctgaa ctggcaaatc tttttttgat tttttattta agctggtttt ttcgtgacgg
    14281 aaatagtagc ccaaaagatc taaaaaaacc attcctgatt acagtaggta taacttttct
    14341 gattttattt cagccaaaga ttgctggagc attgatgatc ctttcgattg cgtgggtcat
    14401 attttgggca gcggcggttc catttaaaaa agggatctat ctaatcgtta ctttttctgc
    14461 attgctgatt ggagcagcag gcggggtatt atatttagga aataaaggtt ggcttccaca
    14521 gatgtttaat catgcttatg aaaggatcgc gactttaaga gattctttta tcgatagtca
    14581 tggtgcgggc tatcaaatga cccattcgtt ttatgcacta tataatggtg gcatatttgg
    14641 gcggggatta ggaaatagta tcacaaaaaa agggtatctt ccagagacag aaacagattt
    14701 tattttttcg attattacag aagagcttgg gctaatcggt gcattgtgtg tgcttttctt
    14761 attgttctca ttatgcatga gaatcttttg tttaagcagt cgctgtaaaa atcagcaagc
    14821 tggattgttt cttttaggat tcggaacttt gctattcgtt cagacgataa tgaacgtggg
    14881 aagtatcgcg gggctgatgc caatgactgg tgtgccattg ccatttgtta gctacggagg
    14941 aacaagttat ctgatccttt ctttaggaat cggaatcacg ttgaatattt cttcaaagat
    15001 acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa taaagagaaa ctgagaaact
    15061 ctcagtttga attatcataa agtacaatct tttatttttc aagcgagagg ttcttttaga
    15121 aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt atctattttg ttactgctta
    15181 cattagtagt tggctatttt tcgattgagt ttatccatgg attttcgtct gcaaaacaga
    15241 gttcaactgt gaaaaaggta gagccgaaaa gtatccctac cacactaaat gtggctttga
    15301 ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc ggattcactt ctggttgcac
    15361 aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat tcatatgtcg
    15421 atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg ggagttgatt
    15481 tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat gcaagtatca
    15541 catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc aagatcgatg
    15601 cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa gtgatggatg
    15661 gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac ttcgggagga
    15721 tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac gtaacttcaa
    15781 tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg aaccttccag
    15841 aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag aataaacagg
    15901 tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg aaaagaagtg
    15961 acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc ttaatagcag
    16021 ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca gtagaagctg
    16081 gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat aacaaagctg
    16141 cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa gagatcttag
    16201 ataaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata tcgaatctaa
    16261 aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag atgaatacct
    16321 cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat gatggtcaaa
    16381 ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat gacaaagtaa
    16441 gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt gaaccattag
    16501 caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga gatggaggcg
    16561 aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa gatgctatca
    16621 atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg aaaatcattg
    16681 atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac ggcagatatt
    16741 atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc acagcagaag
    16801 atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca ggtttggaga
    16861 tggcttttga taaggatctt cgtgggacaa caggtgggaa attaagcatt acagatgcag
    16921 acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa gatatcaaat
    16981 tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga aaagctggat
    17041 caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc tctccaagct
    17101 atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct tatgaagaaa
    17161 atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga tctacgttta
    17221 aaatgattac agcagcaatc ggtctcgaca acggcactat cgatccaaat gaagtgttga
    17281 cgatcaacgg ggggcttaaa tggcaaaaag acagttcttg gggatcttat caagtaacgc
    17341 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
    17401 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
    17461 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
    17521 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
    17581 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
    17641 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
    17701 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
    17761 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
    17821 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
    17881 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
    17941 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
    18001 tttaagtaca gtaa
//
LOCUS       DAFBYI010000055        17689 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFBYI010000055 DAFBYI010000000
VERSION     DAFBYI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.055"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..264
                     /locus_tag="LLD04_001629"
     CDS             <1..264
                     /locus_tag="LLD04_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5648560.1"
                     /translation="HGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP
                     DSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            277..663
                     /locus_tag="LLD04_001630"
     CDS             277..663
                     /locus_tag="LLD04_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648561.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1214..1438
                     /locus_tag="LLD04_001631"
     CDS             1214..1438
                     /locus_tag="LLD04_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648562.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1456..1884
                     /locus_tag="LLD04_001632"
     CDS             1456..1884
                     /locus_tag="LLD04_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648563.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1862..3280
                     /gene="terL"
                     /locus_tag="LLD04_001633"
     CDS             1862..3280
                     /gene="terL"
                     /locus_tag="LLD04_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBK5648564.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3292..4827
                     /locus_tag="LLD04_001634"
     CDS             3292..4827
                     /locus_tag="LLD04_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5648565.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4907..5785
                     /locus_tag="LLD04_001635"
     CDS             4907..5785
                     /locus_tag="LLD04_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBK5648566.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5787..6104
                     /locus_tag="LLD04_001636"
     CDS             5787..6104
                     /locus_tag="LLD04_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648567.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6231..6854
                     /locus_tag="LLD04_001637"
     CDS             6231..6854
                     /locus_tag="LLD04_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBK5648568.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6869..7759
                     /locus_tag="LLD04_001638"
     CDS             6869..7759
                     /locus_tag="LLD04_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBK5648569.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7782..7997
                     /locus_tag="LLD04_001639"
     CDS             7782..7997
                     /locus_tag="LLD04_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648570.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            8009..8350
                     /locus_tag="LLD04_001640"
     CDS             8009..8350
                     /locus_tag="LLD04_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648571.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8350..8721
                     /locus_tag="LLD04_001641"
     CDS             8350..8721
                     /locus_tag="LLD04_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648572.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8714..9109
                     /locus_tag="LLD04_001642"
     CDS             8714..9109
                     /locus_tag="LLD04_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5648573.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9111..9488
                     /locus_tag="LLD04_001643"
     CDS             9111..9488
                     /locus_tag="LLD04_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648574.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9489..10097
                     /locus_tag="LLD04_001644"
     CDS             9489..10097
                     /locus_tag="LLD04_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648575.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10097..10438
                     /locus_tag="LLD04_001645"
     CDS             10097..10438
                     /locus_tag="LLD04_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648576.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10680..13469
                     /locus_tag="LLD04_001646"
     CDS             10680..13469
                     /locus_tag="LLD04_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBK5648577.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13459..14199
                     /locus_tag="LLD04_001647"
     CDS             13459..14199
                     /locus_tag="LLD04_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648578.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14196..16958
                     /locus_tag="LLD04_001648"
     CDS             14196..16958
                     /locus_tag="LLD04_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5648579.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16971..>17689
                     /locus_tag="LLD04_001649"
     CDS             16971..>17689
                     /locus_tag="LLD04_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5648580.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRAT"
BASE COUNT         6296 a         2471 c         3746 g         5176 t
ORIGIN      
        1 catggcaata aggcagaaga cgcgattatt cagatgatgg atatagaaac ggagagagat
       61 gcgattctag cggctttgat ggctcttagt ctgattagtc gtcagatact ctactacagc
      121 ttctgtgtgc cagatagctt ctcaaactac aggattagcc gtgaagtggg ttattcagaa
      181 agaagcatac aaaggatgaa gtcggaagct ctaatagagt ttgcagaagc gtataaacac
      241 ggaagaataa ttgcttataa atagggggac catataatgt ggaaagatta tgtatcgttg
      301 aaagagttga aaaaagatct tgttttcaaa agaattgtag aatggtcaga gagtgaatta
      361 attttagaag atggaaccaa aatggaagtt gtatgtagcg aatcagattg ctgcgcatgg
      421 gccgaaggtg aattcaaaaa tgtaaagctt gatgcagtga ttacagatat aaagattttt
      481 gataaaggta accgtctcta taatggtgac ggacatacct cttatgctga agtcgttgtt
      541 tatcataatc gtaatgagat cgctaaagca gaatgcacag cgaatgatgg gaatggcggc
      601 tactattata gtgtttgtgc tttaaaggtc aaagacaaac tttgtatagt gactgacgca
      661 taaaaaaatg gcggtttttt ggcggaatga tggcggtttt tagccattta tcagtgatat
      721 tatggtagtg tcgaaagatt agtgataggt ctgagacaaa ataataataa aaggaacatc
      781 gttttattat tgtttcacaa ttaagcttcg atagacagca acggaaatat taagaataag
      841 gatgtgaatt ttaactcctt ctaaattgtt cttattatct atcatccgtt gctgtctatt
      901 gttacataca gatcgcttag gcggtctttt tattttggcc gaaaacctac attttcgatg
      961 gccgattatt ggaggaggaa tagcatgttc aaactatctg aaatcattaa gaagtccgac
     1021 gttgagaaat tagagatgtt aaaaaagaag ttgaagaaat agcagtctct tcgtgaggct
     1081 gttttatttt gctcacaaaa atagaccact atcgggtaat agtggtcagg aattaaatga
     1141 aaaagatgtt agggttgtta gctaagtata acatcataac gcttacaaaa caatgcaaga
     1201 aggataaatt attatgagaa cttactggta tgtgtcatta aacaataaat atccgaagcc
     1261 aatgaaagga cagcataggc gtgtagtcat gtctgttcaa atgaaggcga agtattcgat
     1321 tgtagaaatg atcagagagg caacgccagt agagattgat tattgcaagc tagtctattg
     1381 tgggtgcggc cgttggaaag aggatcatgt acaaaaaaat atcagcaagt acatatagag
     1441 aggcggtgat taaggatgga aaaaagcgat acgaaagcga acagatacaa acctactgct
     1501 gcagagaaaa aactcttgga agtattgata aatccagata acttaggcaa accggtgcaa
     1561 gagttgtgtt cactggcgaa aataggtagg accaaatatt acgacgccat gagcaaagat
     1621 ggctttgttg atctagttaa cgaaacaaca atggatctga tcaaaggaaa agcttctgat
     1681 gtccttaatg ccacttacaa gtatgcattg actgaaaaag gtcatcagga taggaaaatg
     1741 attctgacta ttgctggaat ctatgcagac aaacaagaac tcaaacattc tggcggcgtc
     1801 gatatccgaa aacagtatga gaaaatgtcc gatgaagaat tagaagagtt ggtgaaacgg
     1861 tatgaaaaaa tcaacgactc ttaatcgtgt tgagtatgcc gttgttcaaa gagagctagc
     1921 cattcgaaaa gctaggaaaa gtttttggct attctgccag cttctttatc cagagtttta
     1981 caaagatgat cggttgtatt tgaaagattt atgtcatacg ctacaagatt tttacgaaga
     2041 taaaatcgac aaaaaaattt tggttattaa catgccgcct cgacatggga agacttttac
     2101 agcgcggcta ttcgttttat ggatgtttgg acaaaatcca aagacgaaaa ttattaccgg
     2161 atcttataac caaatcctgt catctttatt tgcgcaacaa acacgtgatg gaatcatggt
     2221 tgaaaacgaa aatgttaagg ctgaatactt taaagatatt tttccagagg tgcaaatcaa
     2281 atatggagat gctgcaaagg gtttctggtc tttagatggt tcagaagaaa agaattattt
     2341 agcgacttct cctggtggaa catccactgg tattggcgcg aattatgtaa ttgttgatga
     2401 catcattaaa accgcagaag aagcctataa cgagcgtgtt ttagattctc actgggaatg
     2461 gtataacaac acgttggccc aacgaatgga acggccacgt aaacagatat tgattatgac
     2521 acgttggtca agtaatgatt tagccggaaa aatgttgacg agacgaaaaa ataatgttca
     2581 tcagatctgt tataaagcag tacaagaaga cggctcgatg ttgtgcaacg agattatgac
     2641 ccacgatgag tacttagatg tggtacaaga aatgaatgta gatattgcag aagccaacta
     2701 tcagcaagaa ccaattgacc aaaaaggcag gctatatcag aagtttctta cttatgatac
     2761 gttgcctgat aacattatca aaatatggaa ctacaccgac accgccgaca agggtgccga
     2821 ttactttgcg tctcctgttt ttgctgagac gtctgatcat gatgcttata ttatcgatgt
     2881 catgtataca aaagagccga tggaaacaac tgaaatagcc cacgctaata tgattattcg
     2941 caatcgagtt aataaggtac ggattgaagg aaacaatggc gggcgtggat tcagaagaaa
     3001 ttcagaaaga attacaaaag agcgaggcta tcgaggtgca gtatttgaag actttcatca
     3061 atcggataat aaactgtcta gaatactggc gaatagtgct tgggtggaaa atcatgttta
     3121 ctatccagat gattgggcga cacgttggcc ggacttttat gaggctatgg ttagttatca
     3181 acgtgaaggt aagaacgctc atgatgatgc tccggatgca gttacaggaa ttgcagaaac
     3241 gattatagaa gcagatgact tcgagtttat tgcttattag gaggtgggtc tttgttttat
     3301 tctggaatca cattgaaaaa atacaaacgt ttaagaacga aatattcaac gcaaatcgaa
     3361 gaaggattat tcgatcctaa cgcttttatg gaagatatga aacccttttt cgatgatcga
     3421 gaaagaaagt atcttgctta tactagcgag aaaaacgaga tcgacagtcg tgagaaacca
     3481 aaaacaaaaa tcattaaggt caataacaag attcatgcgg gaatgtattc agtggtcgtt
     3541 gatcaagcag ttaaccattt cacaggaata ccaattaaat gggattatga tgtttcggaa
     3601 cagaaaagga cattcattca acgaatgaga gacaaattac tgggtaatga tatcagcgcc
     3661 ccttctaccc cagaagaatt tgaaacactc acgagacaat taagtactat gcggtttgga
     3721 atgttagacc ccgatactgc aacatttcaa ggagcgacag ggcttgcttt cagattactg
     3781 gagcctgttg agacaaatga tggttgggaa ctaagagcaa gcaacattga gccgtggaaa
     3841 gctgaaaagt acgaaaacgg cgcagtgtac attaaagaga aatacgatcc atatcaaaag
     3901 aagttttatg aagagatgaa ggttgtcacg ccgaaatcaa tatttactta tgctcgatat
     3961 actgattacc ctggcacggt acaaggagca tttaaaacgc tgggtaatgt tcctaatcct
     4021 ttaggcgtta tcaatcttac ggagtttaaa aacaatacaa accgttactg tgactttgaa
     4081 gttgcagagg agattagtga tgctattgat cgtgttttat ctgatcagca gaacgagatt
     4141 gaacaattca agttagctta tatgctgatc actggggcag atttgtccga ggataaagcc
     4201 aagaagatga tggagcaact agggatcatc aatctgaaag accctaatgc gaaagccggc
     4261 tacgtgacaa aggatctaca aaaggatttc aacgagtatc attttgatct tcttaaaaag
     4321 caattctaca cggtgtgtaa agcaattgat ttcaatgacg aagtctttaa gtcgaatagt
     4381 tctggggaag cccgaaagtg gcaaatcatt agtttagaag ctaagacaaa taccaaagaa
     4441 cagtactttc gagaaggatt gaaagagtgt gccgaggtta tggctgctta tttggatttt
     4501 gccgagaaag ttacaattga tccggaaaag atcattttca ccttctctcg ttcgcttcca
     4561 acagatttaa gctacctagc tgaggcattg cctaagttgg caccatacgt ttctaagagg
     4621 acgattcaaa gtcagatccc gttcgtcaca gatgttgatt atgaaaacga aatgattgac
     4681 gtcgaaagca gacaatctta cccagacggc gagtatggag gtggcatcgt tgagtcaaat
     4741 agaaacgaag tactggacca aacgtcgaga actggaagat ttagcaagac tcaaaatgga
     4801 aaatcaaacg ctaaagaaac taagtagtgt ctttcctgat gcgttgaaag aaattcaagc
     4861 ggagttatta tcccaagctg atttacacga tattacctat gctgagatga tggaatttta
     4921 tagtacaagt gatcagaaaa aatatcgtga atacgttgaa aagaactaca aatcactgaa
     4981 gatgtatgat gcaaaataca aagagttcat cgaagaatac tttccttctt tcgactacgc
     5041 caaagtgaat cggctgcttc aaatgaggtc agatattttt cgaattcttg cagagagtgc
     5101 aatagatgca gatgtgaata aatatttttc ggatcggtta gaagatatct tgcaacgttc
     5161 gtatgtatca aacgcaaacg tatttgccca gattttgaac gtagatttgc cgaattactt
     5221 atcaaaggaa gaacttgaac gttacctgaa ctatccttgg tcaggtaaga cattctcgcg
     5281 tcgtttatgg ggcaatattt catctctaga acaaaagcta tctaacgcca ttacaactag
     5341 tgtagcaagt ggcgaaggag ttctcgtggc attacgaaca atgcgagaag acccagaaat
     5401 atgcgatatg ttcaaaaggg aggaatcgaa gtacaacaag gcgatcgaaa atcttgtgag
     5461 aacagaatac gctaaattcg ctcaagatgg tattgagaaa tcttatattg aaactggcgt
     5521 agacgaatac aatgtcttaa ctgcaaaaga tgaaaaggta tgttcaatat gcggtggcaa
     5581 agcaaagaac aacccataca agttgagtga agcgatcatt ggtgagaacc gtgcgccttt
     5641 tcatggccga tgcagatgta cagatgttcc taatatgccg aaattaggaa aagatattga
     5701 cgaagagtat gagcgtttat ttggcgattt attagatgag tttgcacatg attcttttgg
     5761 aattaatttg aaacggagga agtagaatga aagatttttt tgaagcagta ctaacaatta
     5821 atgtaaatgc tgatattgca gaagcctaca aaacagctat tgagtctgag aaccatccta
     5881 atggcttgag agaccattgg aatggcaatt atgcctacgt ggttattggc gatcaaactg
     5941 ttaattatca agataatact ccagttgata agaataccgt taatttaacg attcaattat
     6001 tatctcattc attaccaaat ttaaaagaaa cagttgattg gtatgaaaat atgggatgta
     6061 ttgttgttag aactgactac aaagaaggaa agtctagtaa ttaggctttt ttattttgtc
     6121 cgaaatgacg ttaaactagc gcaatactgg gcttggttga atggtggggc gcaactatta
     6181 aaactcaaag caatgcgggg cgtgaaaacg aatcgtgggg cgaaaggaga atgattatga
     6241 aacacaaatc attaatgcca atgaatttgc aatactttgc cgaaggtgac gatcaaaagt
     6301 tttcttttga tgacttcaaa tcttttgtgg aatccaatga agaagctcaa aaatttattc
     6361 agtcacaatc tcaaagtgtt gccgacaaac aattggaagc ttggaaacaa aataacttag
     6421 ataaaattaa acaggatacc atcaaggaat atgaggaatc taagaaaaac aagtcacctg
     6481 agcaaattca actggaaaaa ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga
     6541 caagtgataa taaagcattt gttgcagaac aaattgctgg attagaacta gatggagagt
     6601 taaaagagtc tatttctcaa tttatgctaa ataatcttgt tagttcggat acagatttca
     6661 ctaagaatgc tgttgaaggt tttacaagtg ttttgaatgc aattaatgag aaacatgcag
     6721 atgcattaaa agaactacaa atgaagtctg catttggtgg aactcaacaa tcgaataacc
     6781 aggttcagca gaacaatgaa acattaacaa atccagaaga acaattagga caaattcttc
     6841 aacaatttaa ctaggagagt gaaaaattat gaaaaaaaca tctttaaata atttagagta
     6901 tttggatatt tcaccagcga ttaatgctat gcaagtacca aatacacctt ttttaagcta
     6961 tttacttggt gctggaaaaa cagagccagc aaactcgaca gaaattaaat ggcgtgaata
     7021 tgatatcaac aacgatgatt cttctgaaaa acttgagggc ggagaatatc cagatgctga
     7081 atcaggtcga acttggttta acaactatac tgaaattttt agaaaatcaa cctctgtatc
     7141 tggtacatta gatgctatta atgtgaatgg agtcggaaat gaattaacta atcaagtagc
     7201 actacgtggt atggaaatga aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc
     7261 tgatgaaaat ggttctaaag gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc
     7321 aaataaggca gaaacagcca ctgcgggtgc agtaacaaga aaagatatcg atactttatt
     7381 taaattgatg tatgaaaaag gttatatggg agaaaaacta tgcttgattt ctccagatat
     7441 gcaggagtta atgactgatg agttagatgg aaaatcaaca aaaattgttc agttcgggga
     7501 aagagtaact tttggattgc aaattggaaa tatcgtgtct aattacggta caggtattgc
     7561 tctactagaa ccatcattgc caaaaggaac aattgccgca atcgatacta attatgtgaa
     7621 actacgtcca ttacgtgaat ggagagcaga agaacttgca aaaacaacgg attctagacg
     7681 tatcggtctt gtaggtgaat actctcttga atacaatgct tcaaactctg gggcaatttt
     7741 aaatttaaag tctgaataaa agggagttag tactcccttt tttgatagga ggaattacag
     7801 tggtaaaaaa agatgaaact aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta
     7861 aaaattttgt tgggtttgtt catcctaaaa ctcgtagatt tatcacagca gattcaaata
     7921 acgaatttat catttctata gatgataaag aagcaattgc aattttggaa gatgcaattg
     7981 atgttaatga aatttaggaa gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga
     8041 gtctacaaaa gaagattttc cagatttgag tgaagaacgc ataactaatt tattagaaat
     8101 aattttgcta gaaattgaat catacaacac ttgtaaaaat gatatttcat gggaaaagtt
     8161 aaaaagcgtg attagtgaag tgttgtataa aataataaaa aatgaatcag aaaaaacagt
     8221 atcttcagtt agacgtggcg atacaacgat tagttatgct tcaacaacaa atgatgttag
     8281 tgagttactt ctaggctatg gtgatttgat acgaagagtt attggttgtg gaggattgga
     8341 gtttttttaa tgaatgaagc agatatttta gaaactactt atgaagatag ttgtattatt
     8401 gaaagactga cggacattga agattctaat acaaatatta ctattcaaga ttacaaaaaa
     8461 gtatatgata atcctatttc ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc
     8521 atagaagatg gagagatggt aaatgtttca actgacacat ataaattatt tgttcatcct
     8581 aagattaaac taaaaaaagg agatcgaata acaataactc aaaaggccag tggcttaatt
     8641 ttttctctat ttgctactaa gcctttttac tatcctagtc attgtgaagt gaatttgata
     8701 ggaagtgaga aaaatgggta atctcaaata tgaatcaaat gcagaaaaga tcattgagaa
     8761 ttttaaaaat atgactgtaa ttgctcaaaa agaaggaata tccttcgtta acgattcaat
     8821 gaataaagtc gttagtttag ctaaaccatt aactcctgta aaatcaggta atttaaggcg
     8881 aggatatcgt gtagtaaaag ctagaaaact atcaagcggt cgaattgttg gagcagttat
     8941 aaacaatgaa cattatttta aatatgtaga agaaggtcat aggactaaaa atggtggctt
     9001 tgtcaaaggg aaattcatgt taactcgtgc aacgaatctt gcaaatatgt cttatattcc
     9061 tcgaagattt aaacaaatgg caataaaaat cgttaagaaa ggaaagtaac atgaaagatg
     9121 aaatcattgc tgcaatcagt agcaaattaa aagaaatcta tcctgatggg acaatatatc
     9181 ttgattcagt tatgcagtca actaaagatt tttatttcgt tttatcagta atggaatctg
     9241 gaactgaaaa tgtaggaatt gatattcaaa atgtttcttt cttaattgat attgcattga
     9301 ttgataataa acctaataga aatttaatta atgaattagt ctcacgttgt gggacttttt
     9361 ttaatacgat tacaatcgat gagcaaatat tatttccaaa agcctattta cctgatgaag
     9421 cagatggtgt tcaacatatt cgttttacat tagaatttcc acaatatatt gaatggagtg
     9481 aaagataaat gggagaaaaa agaagtaaaa ccggaattat ttctgtcgaa aaacctactt
     9541 ggtttccttt ggaagatgag acagggaatt tcccggtata tggtactgcg ttgccaatgg
     9601 gaacagcagt aagtatcaaa ccaacagcta attatgaaac aacacaagat tatggggatt
     9661 cagtggttca agatcaattt acagcttttg gtggtgcaga agttgaactg gaagcaaatg
     9721 gatatactca taaagtttta tctacaatta ctggagggaa aattgttaaa ggtggtgcct
     9781 tgcgttctgg agaagatatt gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga
     9841 atgggaaata ccgttatacg gtcttttata aaggacagtt tgctctagat tcagatgaaa
     9901 cgtcaacgat tgaaggaagt aaagtaagtt ttactcatcc aacttggaag ggttcatttg
     9961 tagatgtacc aggacttgga tatatgtatt cagttgatga agatgatgaa ggcgtggata
    10021 aagcaatgat cggaaattgg tttacaaaag tagcaattcc aattgaagaa gcagaacttt
    10081 caggaggtac agaataatgt caaaatatca aacaacgatt aaattaacga aaaaagatga
    10141 agaaggtaaa tatgagcaag tacaatttaa atctgctgaa tttttaccag gaacagttgt
    10201 agaagatgct gcaggagtta tggaagaaat gcaaacagcg actgataaac aatcagttaa
    10261 aaaagcttta agtcgtgctt attcatttat tgcagatact ttatttgagg gacaatttac
    10321 tggtgaagat tattgcaaag gaattgatgc tcgtgagatt gcctcattga caggaaaatt
    10381 attgaagtct gttactgcag gttttgatga aacttataca gagacgaaaa aaaagtaagt
    10441 gaggcgctca agtcaccttc atttaagtat tcgattactt accgagaatt agatataaag
    10501 acgcaattac tcgaagcagg ttggacgtta ccagaaattg aacgtactga cttggatgag
    10561 ttaatgcgtc tttttgcttt tagagatgcg gtaaaagagc atgaagatgt tgagtactat
    10621 gataatttca ctcaatttta ggaggtgata ctttgaataa cgatgaccta attctgaaga
    10681 tgatactaga tgaatctggt tttactgccg gtatgaataa tgctgtcaaa aagttgaact
    10741 cttttgatga aacaatcgaa agaaaaagta gaaatagtgg cagttcatta ggtagtattt
    10801 ggaaaatatt tgctggaagt tttctagcta gcggagtaac tagaatcgta ggagctggct
    10861 ttgatctaat caagggttcc ataagtggag cggttgatcg agtagatacg atgaataacg
    10921 ctctacgaaa tttccaaaat atgggattta gtaattcaga aattatgaag aatatcggaa
    10981 agaatgggct tttatctcaa ggtattcaag gacttcctac tgctttgaat gatgcgataa
    11041 gtcatgttca acttcttgct tcttctacag gtgatttaac tcgttcaact caaatattta
    11101 aagctttaaa tgatggaatt cttggtttcg gtggttcaac tgatcaagtt aatgaagctg
    11161 ttattcaatt atctcaaagt ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa
    11221 tgatcaacgc tcaacttgga cctactcttt ctgctattgc taaaaagatg ggaattacaa
    11281 tgggagatct gaaagaaggt ttgtctcaag gtaagatttc tgttgaagaa ttccaaaatc
    11341 aattaataga aatggatacc aaaggtggcg gaggacttaa atcattaagt caaatcgcta
    11401 aagattctac taagggaatt aaaacctcta tacaaaatgc aaaaacagct gttacacgtg
    11461 gtgttggtga ggtaatagaa ggattaaata aagcattagt agattcagat ttaggtggat
    11521 ttaagggaat tattgataaa gtgggcagtt caatggaatc gttcttaaaa gtaattgctg
    11581 caaatattcc tacagcagta tcatttttaa gtaatttatt taacgaaatt caaaaatttg
    11641 gttctgcttt gaaattcatg atgccctttc ttgttcctgc agctaccgct tttggagcgt
    11701 tcatgtttca acttaaaggt ataccagcaa taataaaaag cttcaataat tttaagaatg
    11761 ccataattgg cgttggaaat tcattaaaga ttatgggggc aatagctgct gctaattcat
    11821 ttgttttaat tattggtgca gtcgttggtg caatagctgt tttcggttac tttatggcaa
    11881 ccaatgaaga gtttagaaac aaggttatat ctatttggaa tgatgtgaaa gattccgtaa
    11941 ttggtgtatt aaaaaatata aaggactggg gaattgatac ttggaattct gctaaagaaa
    12001 tggcatcaaa tgcagttgag ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt
    12061 ctaacacctg gaaaggtatt aaggatgggg ccacaggttt atttgataaa acagtagaga
    12121 cttcaagaaa tgcagtcgat agtgtaaaaa atgcatggtc taatatgaag aaatggtttt
    12181 ctgatacttg gcaaagtata aaagattcag ccagagaaaa atggaatgaa attaaaggtt
    12241 ctattatgtc ggttgctggt ccattgatta caggtataaa gaatgcattt ttacatgtta
    12301 ctttttatct agagacctta tggaataatt tagttgagat tggtaaaaac gtctttgaga
    12361 ttttaaaaaa tgttatactt gcgccggttt tgtttattac ctccatgatt tctggtggat
    12421 gggaagaaac aaaaaataat atgattgctg tttggaataa tattaaagaa agtgccataa
    12481 atatttggga atctataaag aatatatttg taagttattt tacaaatatt tattttgctg
    12541 cactcaatat ttggacaggg ttcaagctta cattgataaa tatttggaat gaagtggtaa
    12601 atcaagctaa atcgatttgg ttaaacataa agtatttttt tattaatctt tggattgata
    12661 taaaatattt tgcaattcag aaatggattg aattgaagtt ttctattatt caaacttgga
    12721 tcgatttaaa atataatgcc attactactt ggaataatat taaacagttt ttcaaagata
    12781 cttggaaaaa tattaaagat acggcataca atacgtggat ttctataaaa aataccatga
    12841 ttaatacatg gaataacatt aaggactctt tctggaatat tgttactgga attgttaatt
    12901 ctgctgaaaa tgcatggaca aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa
    12961 aagaaatctt taattcgtta agggaagtca atttatttga aattggcaag aacatcattg
    13021 atggacttat aaatggtgta aaagaaaagt ggaatgcttt gaaagatact atcaaaggta
    13081 ttgcaggtaa tatcaaaaat tggattaaag gagctttaga tattcattct ccatcaagat
    13141 ggatgcgtga tatggttggt aaaaatattg ttcaaggtat tatcgttggt attgataaag
    13201 agcaaagtaa gttagatcaa accatgacaa atttagtaaa aacaccgtct gttcaacctg
    13261 tagtaaatgg aataagtcac ctaccaatta cacaaacaaa gcaaaatata tctgctagtg
    13321 aaacaaaaga aattcattta catttaaatg tttatggaga tttacctgat tcaatggttc
    13381 ggaagattac cacaaaaatt aaaacagaat tgacaagaca aatgaaacga gatgctggtg
    13441 cagtaggagg aacattatat gcaacttaag agaggacaat tttttattaa tcaacattat
    13501 tcttctgaat ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc tagtcgagta
    13561 attgaattga gagagcgaga aggaaatgat tctatcatta ttgataaagg ctattacaga
    13621 aatgtgacta gaaagattga gtgttactat aaagcacctt caattgatgt ggtgcaggag
    13681 tgggaagatc gaatcactga atggttagat atgtcgtctt atagtgattt tattctttac
    13741 tatgatgaac aatatattta tcaagctgta gtaatagaag gacctgaatt taaaggaaca
    13801 agaaaaacag gaaatattgt cccttttgaa tttacagtga gcattcgacc atttaaggaa
    13861 aattacaatg gtagatttac tattcgacaa acagaaactt ttgaaattta taacccagag
    13921 aagtattctt caaaaccgct tattaaattg agtggttctg gagatgcttc tttttatatt
    13981 aataaggaca aatatgattt gaaatcgttg gacagagaat tatttataga ttctaaatta
    14041 gaagaggctt accgaaaatt agatggtaat ttagaacatc aggatcaagt cactttattt
    14101 ttagattttc catttttata tcctggaaaa aatgaaatta aatggacgaa aaatattcat
    14161 tcatttgaaa taatgcctag gtggtggaga aaagtatgaa accaagaata tatagtccta
    14221 ctgaaacaga tttctcaacg aatggcttag gaattttaaa ggattgtaca agatgtgaaa
    14281 tatatgaagt agctaatgga aaatatgaat tagagttgga ttatccttta ggaactagat
    14341 ttgatgaata ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat caagaagagt
    14401 atcatatctt ttttattgat gataaagata tagatacttt tttaaataca gtaactattt
    14461 atgctcagag tcgtacaaat cgacttggaa gacgggtagt cactcttgcg gaagtagact
    14521 ctaaaactgg tcaagaagcg atatcaatta ttgaaaccaa aatggataaa aaatctgaca
    14581 tacgacttta ttctgatatt acggccgttt ctagcacaat ctttgaagcg agaaatgttt
    14641 taaattgtat tgctggtgaa caaggatcat tgcttcaata ttggggtgga gaaattaaac
    14701 gtgaaccatt taagctttct ttgttaaagc gaagaggtcg caataatgtt ggaacgattc
    14761 ggtatggaaa agatatgtct ggtttaaagg tcaaattaga ttggacaggt ataaaaacaa
    14821 gacttattcc atatgctgat cctcaaagtg aggtaggtac gactaatcga atttatggtt
    14881 cgccagtaga tagtgcctat attaataact atcctgatgt gtatacagag catgttcagt
    14941 ttacagaaga acaaggagta aaagacatta agagcttaaa taaaatagct aaaaattact
    15001 ttaaaacgat caatcctggc tgtgataagc ctaaagtttc tattacggtt gaatttgata
    15061 agttgactga tagtgaagaa gcgaaagaat ttgcgaagat tagaaattat ggtctgtttg
    15121 atacatttaa aatatatcat aaaaaatata atatttatct tgaatcaaag gttagtggac
    15181 tacaatacga ttctttatct gaaaaaactt tgaaattaga agcaggagac attcaagttg
    15241 ctttttatca gcaacaagct gtaactattc aggataaatt aaaagattat gcaacgaata
    15301 attatatgag tgattttaat gattatgttt cttcaatgat tacaggtcaa ggcagtgcag
    15361 gaggatatgt aagtttatgg ccaaaagaaa aaccttccaa catctttatt atggataatc
    15421 cagatttaga aaaggcaaga gaagtactaa gaatgaataa aaatgggatt gctttttcta
    15481 agaaaggatg gcgggggcct tttacttcag cttggacatt agatagtata tttaatgcca
    15541 attttataca aactggatta atcaaagcag atatctttca aaattctttt aacaagactg
    15601 gagatgtatt gaaattagtt aatggactac tccaaatttg gaacaacaaa aagaaaataa
    15661 tggagttaac taaaaaagga atggaatttt ggaattctaa gggttcaatt ggaacgattg
    15721 gaacaactga ttctgctggt aatccttttc ctggggcttc tactccaacc cctattgaag
    15781 ataattcttt agttattcgt acaaatggag acggcaaata tattttgatt tctcctaaag
    15841 taggaaaagg attagtttta ttaggaaatg gtaaagcaat ttattttgga gacttagatg
    15901 tacaaggaaa acttacagtt aaaggaaaag aaataattcc tggacaaggt ggtggtccat
    15961 cgggaggtgg agaatctact ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga
    16021 gagcttggag aatttacgat attttgtgta ataacggatt tacgaaacaa tctgcatgtg
    16081 gaatattggg gaacattcaa caagaaacag gaggaacttt tgatcctgat actgttcaaa
    16141 taggtggacc agcatacgga ttagttcagt gggatggttc ttcatatcct cttgttggtc
    16201 cagcaacatg ggatggaaaa gtttatgttc aaaacttatt taatgctgca ggtattaaag
    16261 aaccaataac gagcttagat gcacaagttc gattgcttat ttggacattt acaaatggac
    16321 aatggatggg cgtagtacaa cctacgacgg ttgatggatt taaggcctgt actgatccaa
    16381 gacaagcagc atatgctttt gaacgaaact atgaacgtcc ggcagcgaca catcctgaac
    16441 gtcaagatta tgcagttaac tggtataaca aatttaaaga tttaaaacca ggaggagcta
    16501 ctggagaagc aggactaaaa catttagagt ctttgattgg acaacgaatt ggcaatggtc
    16561 agtgttatgg cttgtctgca gaatattcag gatatctggg tggttgtggc atgggtgctg
    16621 gaacaaaata tggtttaact catgtgatag gaaatacttc tgcagcatct gatattggta
    16681 ttgcctatga ttggtctgct gttggttgga aagtgattca aaaccctaga tatgatcaat
    16741 tagtagttgg tgcaattatt aattgggcaa gaggtggaca agtgggttca tggtttgcag
    16801 acggaactta tggacatact ggcgttatcc gaggcttagc taatggtcgt atgcaaactt
    16861 atgaacaaaa cacagagtta ggtatgattt gtggaaagtt agatcgccag tattatagtg
    16921 caagtgcaat ttcttccatt gtcataccac caaaataggg aggtgattta atggcaaagt
    16981 ggaatgtcat attaagtaca acagaaccct ataattacgt ggggatgatt caagttcgac
    17041 aaggcaataa aaacactgaa gttatggaag ctacaattgt cgaaaatggt cttccatatg
    17101 atttatcaga atgtaaggta tattttgaat cagttgttgg cggaaaatat ccagtccaat
    17161 tagaaacaag aattgtggat gctaaaaaag ggaaaatcaa atatattttt gataaatatt
    17221 ccatgcagtg tttgcatcga caaacagcca atttcattat atttaaagga gaagacttga
    17281 ttggaacaac tcaagacttc tcctattttg tcatcaatgc tgtttcaaaa acagaaggag
    17341 aaatgggttc ttattggcaa tcaattgaag atttaattgc tgatatgaca gattttatta
    17401 atgaaaataa aggcgatttt acggactgga tgaatgaaag gaaagaagaa tttgatcgct
    17461 ggagagaaga acaggagaat agtttccaag attggagaga aggacaagaa tcagattatt
    17521 tgtcatggtt tgaatcaatc aaggatattt tgaagtctat cgatccaggt ggagtaatgt
    17581 tagccgaatt aatggatgca cgtgttgata ttcaaggagt tcgtcatgcg tctatttcag
    17641 agcgcttgct ggcagatatg gattatctat atcagaaatt acgagcaac
//
LOCUS       DAFBYI010000056        17688 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFBYI010000056 DAFBYI010000000
VERSION     DAFBYI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.056"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(137..1717)
                     /locus_tag="LLD04_001650"
     CDS             complement(137..1717)
                     /locus_tag="LLD04_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK5648581.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2118..3494)
                     /locus_tag="LLD04_001651"
     CDS             complement(2118..3494)
                     /locus_tag="LLD04_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5648582.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3622..4740)
                     /locus_tag="LLD04_001652"
     CDS             complement(3622..4740)
                     /locus_tag="LLD04_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5648583.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4882..5316
                     /locus_tag="LLD04_001653"
     CDS             4882..5316
                     /locus_tag="LLD04_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5648584.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5394..5927)
                     /locus_tag="LLD04_001654"
     CDS             complement(5394..5927)
                     /locus_tag="LLD04_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK5648585.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="LLD04_001655"
     CDS             complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="LLD04_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK5648586.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7192..7989)
                     /gene="recX"
                     /locus_tag="LLD04_001656"
     CDS             complement(7192..7989)
                     /gene="recX"
                     /locus_tag="LLD04_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK5648587.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8096..9478
                     /gene="rlmD"
                     /locus_tag="LLD04_001657"
     CDS             8096..9478
                     /gene="rlmD"
                     /locus_tag="LLD04_001657"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5648588.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9652..10230)
                     /locus_tag="LLD04_001658"
     CDS             complement(9652..10230)
                     /locus_tag="LLD04_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK5648589.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10552..11022
                     /locus_tag="LLD04_001659"
     CDS             10552..11022
                     /locus_tag="LLD04_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK5648590.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11130..12356)
                     /locus_tag="LLD04_001660"
     CDS             complement(11130..12356)
                     /locus_tag="LLD04_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5648591.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13110..13310)
                     /locus_tag="LLD04_001661"
     CDS             complement(13110..13310)
                     /locus_tag="LLD04_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5648592.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13444..14052)
                     /locus_tag="LLD04_001662"
     CDS             complement(13444..14052)
                     /locus_tag="LLD04_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK5648593.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14137..14607)
                     /locus_tag="LLD04_001663"
     CDS             complement(14137..14607)
                     /locus_tag="LLD04_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK5648594.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14655..15569)
                     /locus_tag="LLD04_001664"
     CDS             complement(14655..15569)
                     /locus_tag="LLD04_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5648595.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15715..16518
                     /locus_tag="LLD04_001665"
     CDS             15715..16518
                     /locus_tag="LLD04_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK5648596.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16696..17643
                     /locus_tag="LLD04_001666"
     CDS             16696..17643
                     /locus_tag="LLD04_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK5648597.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5364 a         3543 c         2911 g         5870 t
ORIGIN      
        1 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
       61 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
      121 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
      181 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
      241 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
      301 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
      361 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
      421 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
      481 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
      541 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
      601 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
      661 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
      721 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
      781 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
      841 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
      901 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
      961 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
     1021 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
     1081 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
     1141 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
     1201 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
     1261 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
     1321 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
     1381 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
     1441 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
     1501 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
     1561 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
     1621 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
     1681 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
     1741 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
     1801 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
     1861 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
     1921 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
     1981 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
     2041 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
     2101 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
     2161 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
     2221 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
     2281 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
     2341 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
     2401 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
     2461 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
     2521 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
     2581 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
     2641 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
     2701 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
     2761 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
     2821 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
     2881 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
     2941 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
     3001 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
     3061 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     3121 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     3181 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     3241 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     3301 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     3361 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     3421 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     3481 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     3541 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     3601 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     3661 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     3721 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     3781 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     3841 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     3901 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     3961 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     4021 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     4081 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     4141 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     4201 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     4261 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     4321 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     4381 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     4441 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     4501 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     4561 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     4621 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     4681 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     4741 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     4801 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     4861 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     4921 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     4981 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     5041 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     5101 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     5161 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     5221 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     5281 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     5341 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     5401 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     5461 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     5521 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     5581 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     5641 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     5701 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     5761 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     5821 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     5881 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     5941 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     6001 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     6061 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     6121 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     6181 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     6241 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     6301 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     6361 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     6421 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     6481 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     6541 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     6601 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     6661 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     6721 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     6781 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     6841 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     6901 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     6961 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     7021 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     7081 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     7141 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     7201 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     7261 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     7321 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     7381 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     7441 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     7501 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     7561 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     7621 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     7681 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     7741 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     7801 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     7861 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     7921 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     7981 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     8041 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     8101 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     8161 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     8221 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     8281 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     8341 cggtggctgt caattacaac acttagctta tcaagcgcaa ttggatttta aaaaggatct
     8401 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     8461 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     8521 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     8581 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     8641 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     8701 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     8761 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     8821 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     8881 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     8941 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     9001 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     9061 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     9121 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     9181 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     9241 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     9301 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     9361 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     9421 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     9481 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     9541 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     9601 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     9661 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     9721 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     9781 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     9841 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     9901 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     9961 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    10021 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    10081 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    10141 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    10201 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    10261 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    10321 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    10381 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    10441 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    10501 cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa aatgaagatg
    10561 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    10621 gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc accatttgca
    10681 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    10741 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    10801 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    10861 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    10921 ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    10981 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    11041 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    11101 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    11161 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    11221 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    11281 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    11341 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    11401 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    11461 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    11521 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc aggggtgtag
    11581 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    11641 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    11701 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    11761 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    11821 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    11881 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    11941 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc atgatttaat
    12001 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    12061 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    12121 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    12181 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    12241 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    12301 atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    12361 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    12421 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattact attttttaaa
    12481 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    12541 cgcagttcgc aatcgtttta tcttttttta cgtatttttt ccagaaaaag cgaacaacaa
    12601 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    12661 agtctttgcc taccgttcag attatcattc gatttcacga aaatttcttc ctatgactgg
    12721 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    12781 atgactcgaa ctagtgattt cattaccatc atcttttaat ggatagatat gctcctcttc
    12841 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    12901 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    12961 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    13021 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    13081 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    13141 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    13201 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    13261 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatag
    13321 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    13381 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    13441 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    13501 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    13561 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    13621 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    13681 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    13741 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    13801 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    13861 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    13921 ctcaattgct gcatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    13981 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    14041 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt ataatgtcta cattacaaag
    14101 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    14161 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    14221 cgctaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    14281 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    14341 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    14401 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    14461 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    14521 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    14581 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    14641 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    14701 tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa
    14761 gaaattatac gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata
    14821 tgtccgtcga aaataattat cttttcgcta tactgccaaa tatggacatg atgaacattc
    14881 tttaccccat cgattgcttc aatttcttgt tccatttttt catagtcaat agcaggtgca
    14941 ctttgtatca aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat
    15001 atcgaaatga tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa
    15061 attgctccga taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta
    15121 gattttatat tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc
    15181 gcgagcaatc caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa
    15241 gtgaaccgct tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag
    15301 ttgatgaaag cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct
    15361 tcttttcggg ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg
    15421 ttatggaaag catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct
    15481 gataaagtga tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt
    15541 ggctgtgtag gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga
    15601 ataacatttt tttgatattt caccaatcat tcagatgttt ccctaaagca cttgtatttc
    15661 acttgatact tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca
    15721 acaaaacaat taattatcag ttcctttata cgattcaaag acctaaagga gacgagaaat
    15781 gtcccatttt ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca
    15841 atctcttttc aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct
    15901 actcgcattc ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga
    15961 ttcatttacc aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa
    16021 caggacatct ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa
    16081 cttatcatag ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta
    16141 gaacttccct acaccaatga atctttaaaa aacttaacag gtaaggagct ccgttctttt
    16201 ttaagtaaag catctatacc actttggata aaagtcctga catttatatt ttctatttat
    16261 cctagttttc tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat
    16321 gcacgctttc gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca
    16381 atttcattgc ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacaca
    16441 agcgccttca ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca
    16501 aaaatatcga tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt
    16561 tagaggttgt gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc
    16621 aaaaaacaag caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa
    16681 ggcgggaaat taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa
    16741 tttatcgaag aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca
    16801 gaggatctac aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta
    16861 ttagcctctt ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc
    16921 cttgagactt tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa
    16981 tcgattacgg agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca
    17041 caaaaagcac agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa
    17101 ggaaaaaaaa tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc
    17161 aaagcattag acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg
    17221 gaaaagactt tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc
    17281 aatatcttac ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac
    17341 tttgatccaa aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat
    17401 ctggtgcagg ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa
    17461 gagcctcttc cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac
    17521 attgcaggtt tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg
    17581 aaaagctact ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat
    17641 taatagctga aaacaatatt ttcaaaagaa caaagagaag gtgacaaa
//
LOCUS       DAFBYI010000057        17440 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFBYI010000057 DAFBYI010000000
VERSION     DAFBYI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.057"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            365..1276
                     /locus_tag="LLD04_001667"
     CDS             365..1276
                     /locus_tag="LLD04_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK5648598.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1493..1930
                     /locus_tag="LLD04_001668"
     CDS             1493..1930
                     /locus_tag="LLD04_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648599.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2025..2909
                     /locus_tag="LLD04_001669"
     CDS             2025..2909
                     /locus_tag="LLD04_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5648600.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3133..6300
                     /locus_tag="LLD04_001670"
     CDS             3133..6300
                     /locus_tag="LLD04_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648601.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6320..6865
                     /locus_tag="LLD04_001671"
     CDS             6320..6865
                     /locus_tag="LLD04_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBK5648602.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6893..7348
                     /locus_tag="LLD04_001672"
     CDS             6893..7348
                     /locus_tag="LLD04_001672"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBK5648603.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7345..8103
                     /locus_tag="LLD04_001673"
     CDS             7345..8103
                     /locus_tag="LLD04_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5648604.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8189..8929)
                     /locus_tag="LLD04_001674"
     CDS             complement(8189..8929)
                     /locus_tag="LLD04_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648605.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8990..9274
                     /locus_tag="LLD04_001675"
     CDS             8990..9274
                     /locus_tag="LLD04_001675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648606.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFLLSLDSAFFWFLI"
     gene            9556..10242
                     /locus_tag="LLD04_001676"
     CDS             9556..10242
                     /locus_tag="LLD04_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5648607.1"
                     /translation="MLISFQVKERQHQESLQLYQINEEYYHHLEEFKHDYKSLLFSLK
                     ITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIP
                     IQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIAN
                     KANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMY
                     LASRKSKSAY"
     gene            10564..10791
                     /locus_tag="LLD04_001677"
     CDS             10564..10791
                     /locus_tag="LLD04_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5648608.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10914..11240
                     /locus_tag="LLD04_001678"
     CDS             10914..11240
                     /locus_tag="LLD04_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5648609.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11308..12021
                     /locus_tag="LLD04_001679"
     CDS             11308..12021
                     /locus_tag="LLD04_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5648610.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12331..13182
                     /locus_tag="LLD04_001680"
     CDS             12331..13182
                     /locus_tag="LLD04_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5648611.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13303..15168
                     /locus_tag="LLD04_001681"
     CDS             13303..15168
                     /locus_tag="LLD04_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5648612.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15216..16625
                     /locus_tag="LLD04_001682"
     CDS             15216..16625
                     /locus_tag="LLD04_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK5648613.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16733..17434
                     /locus_tag="LLD04_001683"
     CDS             16733..17434
                     /locus_tag="LLD04_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5648614.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         5912 a         2905 c         3705 g         4918 t
ORIGIN      
        1 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
       61 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
      121 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
      181 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
      241 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
      301 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
      361 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
      421 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
      481 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
      541 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
      601 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
      661 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
      721 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
      781 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
      841 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
      901 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
      961 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     1021 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     1081 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     1141 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     1201 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     1261 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     1321 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     1381 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     1441 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     1501 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     1561 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     1621 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     1681 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     1741 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     1801 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     1861 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     1921 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     1981 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     2041 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     2101 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     2161 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     2221 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     2281 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     2341 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     2401 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     2461 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     2521 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     2581 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     2641 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     2701 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     2761 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     2821 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     2881 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     2941 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     3001 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     3061 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     3121 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     3181 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     3241 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     3301 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     3361 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     3421 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     3481 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     3541 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     3601 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     3661 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     3721 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     3781 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     3841 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     3901 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
     3961 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
     4021 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
     4081 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
     4141 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
     4201 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
     4261 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
     4321 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
     4381 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
     4441 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
     4501 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
     4561 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
     4621 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
     4681 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
     4741 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
     4801 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
     4861 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
     4921 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
     4981 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
     5041 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
     5101 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
     5161 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
     5221 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
     5281 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
     5341 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
     5401 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
     5461 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
     5521 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
     5581 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
     5641 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
     5701 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
     5761 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
     5821 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
     5881 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
     5941 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
     6001 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
     6061 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
     6121 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
     6181 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
     6241 atagctggta attactcagc agagataaca tggacattag cagatgcgcc gacagaataa
     6301 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
     6361 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
     6421 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
     6481 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
     6541 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
     6601 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
     6661 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
     6721 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
     6781 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
     6841 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
     6901 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
     6961 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
     7021 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
     7081 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
     7141 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
     7201 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
     7261 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
     7321 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
     7381 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
     7441 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
     7501 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
     7561 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
     7621 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
     7681 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
     7741 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
     7801 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
     7861 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
     7921 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
     7981 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
     8041 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
     8101 taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct
     8161 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
     8221 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
     8281 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
     8341 attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag
     8401 tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga
     8461 tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa
     8521 taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt
     8581 gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc
     8641 gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg
     8701 catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga
     8761 tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt
     8821 gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga
     8881 tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc
     8941 tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac
     9001 agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc
     9061 gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt
     9121 acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt
     9181 ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt ttttgctatc
     9241 actggactca gcttttttct ggtttttgat ctgatggatt atttaggaaa gagacatctt
     9301 ggaacagcaa gtttttttgc actacttatc gcatttattt tctatctatt agtaaaaagt
     9361 atagacaaac agacgagatt tctcaacttg ctttttactt atccaactga acactatttt
     9421 ggaggtattt gtattttatt aagctggggc gtgatttact tggtattggg aagtgttcac
     9481 ttccgctcat ctaattatct gcttctatct cttttgtcga tagtcatcag ttttttttct
     9541 ttgttttttt ccattatgtt gatcagtttc caagtaaaag aaagacaaca tcaggaatcg
     9601 cttcaactgt atcaaataaa tgaagaatat taccatcatc tagaggaatt caaacatgat
     9661 tataaaagtc tactgttttc attaaaaatt actcttcagg aaaataaaaa agaaacgttg
     9721 gaatttttag aaatattaga agatcattct gcagctattt ttgaaaaacc gcaaatacaa
     9781 cagctaacaa atattgtatc cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa
     9841 gcagaatcgg agaaaatccc gatccaagta gttattcctg tcgaagtgaa aacaatcccg
     9901 attgatctgg ttgtttttat tcgctgttta tctattttta tttctaacgc attcgatcat
     9961 cgtgtagccg atcagagtcc gattacgatc aatctgctgg aagaaaaaga gggatcacgt
    10021 tttagtatag cgaacaaggc aaatccaacg aatttgacgc tatcggagat ggtcaaaaga
    10081 ggaaaaacct ctaaaaaaaa tggcgggtta ggtctataca gtgcgaaaaa aatgttggat
    10141 gtttacgaaa atgcagaact aaaaatcgaa ttttcaaaac agaatcacta tttttttgta
    10201 gaaatgtatc tggcatctag aaagagcaaa tcagcctact gacttactgt caaaggataa
    10261 gtttgaggaa agtctacacg gagatagaga caacaacaca gcatattcaa aagaaacaga
    10321 agcgtgatga ccgttgtcac ccttctgttt cttttgctat gtagttaaac aaagtagttg
    10381 acacttccgc tacttagtat tagtacgaaa gtatacttca tgatactaag tagataagaa
    10441 gtgaaaagcg gaacccacga tcgtcaaaat gctaactgct ttgcttacac cagataaagg
    10501 gaagagcaat atcgcttatt ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa
    10561 aaaatgctga cccagcagtt aagagagtta gaacaagatg aaagtatcca tcgaaaagcc
    10621 tataaccaag tcccgcctaa agtagagtac tcgctcaccg aagaaggaaa atcattaaga
    10681 gagattctag tagcgttgtc tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca
    10741 agggaagaag acacgctact gcatacacat ggtggctacc taaattattg attttctatg
    10801 acatttattt taaataaaag aaaacaggag tttttttaaa agcatcatct attttttatg
    10861 taaaaaaatg gtatgattaa tcaagtaaga agtgattgga agaaggcaag caaatgaata
    10921 gtatggaaaa agtcaaccag tttagagatg aacgtaattg gcgacaattt cataacgaaa
    10981 aagatttggc tatctctatt tctttggaag catctgaatt gttagaactt tttcaatgga
    11041 aaacgccgga agaagtaaaa gaaacacaat tagaacgaat caaagaagaa ctggcagatg
    11101 tattgatcta ttcatacatg atggcagaca accttcacct ggatctagat gagatcattg
    11161 aagaaaaatt gatcaaaaat aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa
    11221 aaaaatacac agagttgtag aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac
    11281 agtaaaatga gtctggaggg agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta
    11341 gtcaatatcc tcttgttcct cttgttatta gtaggactag ctttgatttt caatgaacag
    11401 atcaaggatt attttgtcag agagacagga gataaatatg ctatagcgaa tgtaacaaaa
    11461 gaagatctga aaaagaataa cgataaagat gtcagttttg actttgatgc ggtagaacca
    11521 atgacgacag aaggggtcat gaggtcgcaa atgagaggaa cagatctacc agtgattgct
    11581 agtatcgcag tcccttcagt ttcaatcaat ttaccggttt tcaaaggctt ggataatacg
    11641 tcactacttt acggagcagg aacattatct ccagatcaag aaatgggaaa aggaaattat
    11701 gccttagcaa gccatcgtgc aacgaaccct gaattgctgt ttacaccgct agaaaattta
    11761 gagatgggtg ccaagatcta tttgacagat ttagaaaatg tgtatacata caaaacattt
    11821 tttaaagaaa aagttgctcc tacagatacc caattactga atgaagtcga gggaaaagaa
    11881 attgtcacac taattacttg tggcgacatg gacgccgtca cgcgactagt tgtacaagga
    11941 gaactggaaa gtgttacatc gataaaagat gcgacagatg atatgagatc tgctttcaat
    12001 ctagagacaa aaacatttta ggatactggg acataagtcg ttacagatta aaaacagcca
    12061 aaggatatga atcgataagg aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt
    12121 gcatctctga tttggttgtt ttctagatag acttgtgttc cagttttttt attttttata
    12181 tgaaaaggct tgcataaata aaaattcgat ggtatgatga ttttgaataa gaaatggatt
    12241 gttactgtac ggcaggcaaa accagaattc agctaagaaa cagtaggcga attctaggtt
    12301 tttttatttt tcttgagagg gcgaagcaaa atgaaaattg ataaaatttt gaataacaat
    12361 gtcgtcattt cgaaaaatgg attcggggaa gaagtcgttt gtatgggaaa aggactagct
    12421 tttcaaaaaa agattgggga cgagatttcg ccagaagcgg tccaaaaaga atttgtttta
    12481 agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag
    12541 atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg
    12601 cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa
    12661 aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat
    12721 caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag
    12781 gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg
    12841 caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc
    12901 ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag
    12961 ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac
    13021 gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat
    13081 cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat
    13141 attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc
    13201 aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct
    13261 gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt
    13321 ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt
    13381 gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa
    13441 gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc
    13501 agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag
    13561 gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata
    13621 tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg
    13681 gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgaa
    13741 gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc
    13801 aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca
    13861 gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca
    13921 tctggttata cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta
    13981 gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc
    14041 gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc
    14101 ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt
    14161 ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcatcg gggattcgta
    14221 ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt
    14281 ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg
    14341 aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca
    14401 actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga
    14461 ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc
    14521 agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca
    14581 gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta
    14641 ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa
    14701 ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg
    14761 agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat
    14821 ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact
    14881 ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg
    14941 gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc
    15001 aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc
    15061 gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac
    15121 caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag
    15181 gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat
    15241 tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag
    15301 gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa
    15361 aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg
    15421 ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt
    15481 ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga
    15541 ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc
    15601 ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg
    15661 aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt
    15721 tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca
    15781 atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta
    15841 gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta
    15901 tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc
    15961 aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat
    16021 acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa
    16081 aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt
    16141 ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag
    16201 gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc
    16261 tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa
    16321 acggtcttgg agccattgat catatggaag aggatggcac gattattgat gattatcgga
    16381 tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt
    16441 tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga
    16501 gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc
    16561 gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact
    16621 tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt
    16681 tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag
    16741 gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa
    16801 atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag
    16861 caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa
    16921 aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc
    16981 aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt
    17041 gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt
    17101 ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct
    17161 tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt
    17221 gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt
    17281 aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg
    17341 gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga
    17401 gctgcatcct taccatattg attatgaaac ataaataaaa
//
LOCUS       DAFBYI010000058        16777 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFBYI010000058 DAFBYI010000000
VERSION     DAFBYI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.058"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..832)
                     /locus_tag="LLD04_001684"
     CDS             complement(<1..832)
                     /locus_tag="LLD04_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5648615.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="LLD04_001685"
     CDS             complement(846..1643)
                     /locus_tag="LLD04_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5648616.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="LLD04_001686"
     CDS             complement(1676..1960)
                     /locus_tag="LLD04_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK5648617.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="LLD04_001687"
     CDS             complement(1957..2958)
                     /locus_tag="LLD04_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK5648618.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="LLD04_001688"
     CDS             complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="LLD04_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK5648619.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="LLD04_001689"
     CDS             4026..4874
                     /locus_tag="LLD04_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5648620.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5520..5726
                     /locus_tag="LLD04_001690"
     CDS             5520..5726
                     /locus_tag="LLD04_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK5648621.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5928..6929)
                     /locus_tag="LLD04_001691"
     CDS             complement(5928..6929)
                     /locus_tag="LLD04_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5648622.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6934..8847)
                     /locus_tag="LLD04_001692"
     CDS             complement(6934..8847)
                     /locus_tag="LLD04_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK5648623.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(9015..9521)
                     /locus_tag="LLD04_001693"
     CDS             complement(9015..9521)
                     /locus_tag="LLD04_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5648624.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9681..10121
                     /locus_tag="LLD04_001694"
     CDS             9681..10121
                     /locus_tag="LLD04_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5648625.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10147..11304)
                     /locus_tag="LLD04_001695"
     CDS             complement(10147..11304)
                     /locus_tag="LLD04_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648626.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11307..11663)
                     /locus_tag="LLD04_001696"
     CDS             complement(11307..11663)
                     /locus_tag="LLD04_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648627.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11961..12935)
                     /gene="sgrA"
                     /locus_tag="LLD04_001697"
     CDS             complement(11961..12935)
                     /gene="sgrA"
                     /locus_tag="LLD04_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK5648628.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(13131..13970)
                     /locus_tag="LLD04_001698"
     CDS             complement(13131..13970)
                     /locus_tag="LLD04_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5648629.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14155..15042
                     /locus_tag="LLD04_001699"
     CDS             14155..15042
                     /locus_tag="LLD04_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK5648630.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15626..16321)
                     /locus_tag="LLD04_001700"
     CDS             complement(15626..16321)
                     /locus_tag="LLD04_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK5648631.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16305..16703)
                     /locus_tag="LLD04_001701"
     CDS             complement(16305..16703)
                     /locus_tag="LLD04_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5648632.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         5014 a         3414 c         2578 g         5771 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgcca catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat accgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taagtaactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa catcaatagt
     4981 cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt attattcgag
     5041 ttacttgtta gatgcgataa caaagttagt atacagtagt aaaaaattta tcttttcttt
     5101 tttttattat ttttgtttct tattttctaa aaaattaaaa aaaattttgg tgaattaagt
     5161 atttccttga taacactggc ttttggcctc attttaggtt atcatttgta attttatttc
     5221 acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa tttttgggga
     5281 aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc tattattggt
     5341 ctagacgtat gatggtaaac tttgtaccta tctgattgtt gaatcattaa ttgcttatca
     5401 ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct aacaaacttg
     5461 tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag gtgctgctta
     5521 tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta tttgctattc
     5581 atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca gcattctttt
     5641 gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt gttgcttttt
     5701 ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga tcgtgacaat
     5761 aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc taactcctca
     5821 gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt gctccttgtg
     5881 acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta ctccttgaaa
     5941 aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc aaactgatat
     6001 tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt cacccccgct
     6061 ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt atttccatcc
     6121 gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa acttttgacc
     6181 ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt ttcataaaga
     6241 aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag accagacaaa
     6301 gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc agctttttta
     6361 atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc tgccgaattt
     6421 ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt agctgcttga
     6481 atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga tcgttcataa
     6541 tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata atcacgacaa
     6601 tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat cggtaatttt
     6661 ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt tgaaggatag
     6721 gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc tggtcgggca
     6781 tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct aatcggcttt
     6841 ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat aactggtact
     6901 gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc gccaaatcca
     6961 tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg aatgtttctt
     7021 ttctccagtt ttcggcaaca ttcttttttt tgcatctgtc tttttttgta ctttcactgt
     7081 catctgaatc ggttgcgtat catcacttga ttgatcctct cgaggttcat tctttccctt
     7141 attgtctgca ttctctgtcg gatcttgctt ttttggtgga gctactggga tttttttgta
     7201 atagaaaata atagttggta tcgtctcatt aaaaatccct tcagtcggtc cttcaacatt
     7261 tgctaattcg tatccttcga atgtcttttg ctctgtcata taattttctc cataagctcc
     7321 gtaaagctgg atttcgtcct ggtctttgag tagactttta ttgttttcat ccacatactt
     7381 tacgtttatc tgatcatgga acactacttt caaaaagtcc gttacatgtt tcgtgtaggc
     7441 agcatggtca acttcctgtg cctttccatg ccctgctcct tctactaata aaagatcttt
     7501 ataccctcct gataactcat ataatttttg cgcgttagac acaggtacta catcatctgc
     7561 tgttccatgg ataatcaatt taggcaatga atctgacatt ccaatcgtag gtagagcagc
     7621 ttttgtatcc aaatgcatct cctgatcaaa atagtattgt ttcaaaaggc ccataaataa
     7681 atcttcatgc tcttgatccc caatgatgtc taactgattt tttatggcat ccggtagtgc
     7741 gggggctact gtctggctgt acgtaaaacg tagttgttca tcaattgatg caaatccgca
     7801 attttcaata attcctttga cattgacagg aatatctttc gctaatacag acatcatcgt
     7861 agctgctccc atcgaaccac cataaagtaa aatcttttga gatggccgct ctcgaacttc
     7921 ttgatttatc cagtaaagga cgtcatctga ctcatattgt ccataggtca cataatcgcc
     7981 tccgctatta ccagtcgctc tggaatcaac aaataataaa ttatacccgg ccttataaaa
     8041 atcattgtat tcttctgtaa cgatcccgtt attccaattc cctcgaaatc cgccatggat
     8101 catgactgtt ttatctgaac cttgatcgac ataatatgca tatacatcca tcattttgcc
     8161 atcttgtttg tttagtacag tttgatgaag ttgttttgta tttggataga agcctgtgtt
     8221 ttctgtaggc gttgtcccat catctgtacc tccaagcatc gacatcaaca cactgttttt
     8281 tgcaaaagcg atatcgtaca atagataagt agcaatcaga tgtttgataa tctctttatc
     8341 tacaccataa tcattaacag caagctcagt gactaataac tctattaatt ttgacttgat
     8401 gtcttctgtt ggtccattaa gtatttctgt catctgttta ccgaagtaat tcaaatattc
     8461 tttaccattg aaattttcac ctttaccgat atcatcattt tctgtcccat tctctttgtc
     8521 acttacttta gcagaagagg attcgtcgtt tgacattttt gtagctgttg cttcttctgt
     8581 ttgttggtta tctgtggtag cggtcgtttc ttgatctgtg ctagtaacag atgtctcaac
     8641 cgttgtttca gtatttgttt ctgtttgtga agtactcgat ttcgtttcat ttccagcatc
     8701 agtagtttct gtggtttcta cctcccgaga ttccgattcg gaagaaatgc tctctccata
     8761 tactgtccat tgcgggacgc ttgtacttac agcaaataag atgaccaacg ctccctttaa
     8821 tacttgtttt gttctcaagc tattcactcc tttttatgtt tctaggagaa ttatatgtaa
     8881 acgcaaacat tttttataca aaaaataaat ggtttatccc ataaaaatag aacaggatta
     8941 ttttctcatt ttacgtaata aagcagaatg gataaaccag tatccattct gcttttttta
     9001 aacgaagaaa caacttattg ctccaaaaat gtcagaaatc gtttatccca aattttctcg
     9061 taaaaagcga ttgtttcctt tgctttcata aaaggattat cataagttgt cgttgctcct
     9121 ctacacatca taagctggta tcctaatcca tgagcaaact tcacagtcgt atctatacaa
     9181 tactctgctt gtgccccaca taattcgatc gaatgacagt ccaattcagt taataaaaca
     9241 ctcaaattag tcttataaaa tgaattcgca tgggtttttc tcacaaaata actgtgctca
     9301 ggaatagata attccgacaa tagctcccat ggttttgttt gtggcactaa ttgagcatct
     9361 tcgtgttgaa cgaaaataac tggtttccca atcgtttcgt atgttttgat tcttttattc
     9421 actaatgctg tcactgtttc taattcgtgt atcggcgaag aatcaaaaca tacccctttt
     9481 tgtaaatcga tcactactat tgcgtcggct gtgtttgaca tacttgtgcc ttctttcttt
     9541 tttcttgatt taatagtatt tcttatctag ctagttccat tatacgtgac aacattttct
     9601 tattttacac ctctaaatgg cacttttcga ctatttcttc atgttattat ttcataaata
     9661 cttagctatt ggaggaatgt atgatgatta aattgtatac aggttctggc tgctcgtctt
     9721 caagacgcgc aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa cagaacttaa
     9781 ttcgtcaacc actaaccaaa aatgaattta tccacttact aaaattgaca aacaacggcg
     9841 tgaccgaaat gctctctact cattctatta gttataaaaa tcttgttcac agaatagatg
     9901 aactcagttt gagtgaacta tatattgaga ttcgtacatc cccttcttta ttgaaaaaac
     9961 cgattatcta cgaccaatct gcaaaacttt tgactggatt tagcgaagat aaaatcagga
    10021 tgttcctttc taaaactaaa agaaagcaag agctcaacga actcttgctt tctggtcatc
    10081 ttgtcgattt taaagaaaat aatctttttg caatgtctta attttggcat aaacaatctg
    10141 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
    10201 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
    10261 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
    10321 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
    10381 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
    10441 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
    10501 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
    10561 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
    10621 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
    10681 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
    10741 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
    10801 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
    10861 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
    10921 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
    10981 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
    11041 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
    11101 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
    11161 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
    11221 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
    11281 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
    11341 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
    11401 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
    11461 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
    11521 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
    11581 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
    11641 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
    11701 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
    11761 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
    11821 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
    11881 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
    11941 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
    12001 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
    12061 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
    12121 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
    12181 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
    12241 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
    12301 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
    12361 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
    12421 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
    12481 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
    12541 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
    12601 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
    12661 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
    12721 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
    12781 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
    12841 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
    12901 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
    12961 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
    13021 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
    13081 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
    13141 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
    13201 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
    13261 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
    13321 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
    13381 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
    13441 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
    13501 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
    13561 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
    13621 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
    13681 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
    13741 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
    13801 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
    13861 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
    13921 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
    13981 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
    14041 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
    14101 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
    14161 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
    14221 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
    14281 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
    14341 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
    14401 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
    14461 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
    14521 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
    14581 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
    14641 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
    14701 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
    14761 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
    14821 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
    14881 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
    14941 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
    15001 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
    15061 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
    15121 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
    15181 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
    15241 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
    15301 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
    15361 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
    15421 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
    15481 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
    15541 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
    15601 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
    15661 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
    15721 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
    15781 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
    15841 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
    15901 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
    15961 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
    16021 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
    16081 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
    16141 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
    16201 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
    16261 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
    16321 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
    16381 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
    16441 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
    16501 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
    16561 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
    16621 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
    16681 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
    16741 tttttattca aaatgtttct agattataaa atttatt
//
LOCUS       DAFBYI010000059        16660 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFBYI010000059 DAFBYI010000000
VERSION     DAFBYI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.059"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            92..421
                     /locus_tag="LLD04_001702"
     CDS             92..421
                     /locus_tag="LLD04_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5648633.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            411..698
                     /locus_tag="LLD04_001703"
     CDS             411..698
                     /locus_tag="LLD04_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5648634.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYDQLKKLI"
     gene            984..1841
                     /locus_tag="LLD04_001704"
     CDS             984..1841
                     /locus_tag="LLD04_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5648635.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1842..2390
                     /locus_tag="LLD04_001705"
     CDS             1842..2390
                     /locus_tag="LLD04_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648636.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2687..3634)
                     /locus_tag="LLD04_001706"
     CDS             complement(2687..3634)
                     /locus_tag="LLD04_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBK5648637.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            3822..5009
                     /locus_tag="LLD04_001707"
     CDS             3822..5009
                     /locus_tag="LLD04_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBK5648638.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            4990..6294
                     /locus_tag="LLD04_001708"
     CDS             4990..6294
                     /locus_tag="LLD04_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK5648639.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            6320..7333
                     /gene="citC"
                     /locus_tag="LLD04_001709"
     CDS             6320..7333
                     /gene="citC"
                     /locus_tag="LLD04_001709"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBK5648640.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            7337..7630
                     /gene="citD"
                     /locus_tag="LLD04_001710"
     CDS             7337..7630
                     /gene="citD"
                     /locus_tag="LLD04_001710"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBK5648641.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            7635..8540
                     /gene="citE"
                     /locus_tag="LLD04_001711"
     CDS             7635..8540
                     /gene="citE"
                     /locus_tag="LLD04_001711"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBK5648642.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            8533..10092
                     /gene="citF"
                     /locus_tag="LLD04_001712"
     CDS             8533..10092
                     /gene="citF"
                     /locus_tag="LLD04_001712"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBK5648643.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            10089..10646
                     /gene="citX"
                     /locus_tag="LLD04_001713"
     CDS             10089..10646
                     /gene="citX"
                     /locus_tag="LLD04_001713"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBK5648644.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            10639..11502
                     /gene="citG"
                     /locus_tag="LLD04_001714"
     CDS             10639..11502
                     /gene="citG"
                     /locus_tag="LLD04_001714"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBK5648645.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            11736..12145
                     /gene="tnpA"
                     /locus_tag="LLD04_001715"
                     /pseudo
     CDS             11736..12145
                     /gene="tnpA"
                     /locus_tag="LLD04_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12782..13954)
                     /locus_tag="LLD04_001716"
     CDS             complement(12782..13954)
                     /locus_tag="LLD04_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5648646.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            14628..15440
                     /locus_tag="LLD04_001717"
     CDS             14628..15440
                     /locus_tag="LLD04_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648647.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT         5810 a         2505 c         3143 g         5202 t
ORIGIN      
        1 tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta tttactaagt
       61 aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa aacaaggcaa
      121 ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg aagttgaagc
      181 aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag aattttatga
      241 ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag atgagattct
      301 tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata ttgctgaaga
      361 tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa ttggcttatg
      421 aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag aagccattga
      481 aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat aagggtgaac
      541 aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg ggcacaacat
      601 atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta tgtggaacgc
      661 atgaaaactt ttatgatcag ttgaaaaaat taatctagtg atttgttgtc gttcttaaga
      721 aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
      781 ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata aatcaatata
      841 tttcagcttt tccttattta tgtactacct tttccttatt aattataagg tagtacatat
      901 atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca aatgaatatt
      961 gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt aattttaaag
     1021 gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag
     1081 gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat gtgatgaact
     1141 taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg attgtttacg
     1201 aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct gtctataaag
     1261 taatcaataa cctctatata cttccagcag atatgtcctt tgaaatgtat gatgattgga
     1321 tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa aagctagaac
     1381 cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt tctgtatata
     1441 gtaaatccgc gatgtacatt gcggactggg caatcgtcgt cttacagacc caagtaaaat
     1501 cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa gaatttgata
     1561 caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac gcagtagatc
     1621 aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa aatgttgtac
     1681 tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag aagacaaaga
     1741 aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt attttgaaag
     1801 aattggaaga acacgagaat tggtatgcgg aggtggagta attggctgac atcaaaggac
     1861 ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga
     1921 aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac caagttcaag
     1981 aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa aaagttggcg
     2041 caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa ctgtcgactt
     2101 tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg acaagagatg
     2161 aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa gaagataagc
     2221 aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa tatccaattg
     2281 ttccagaatt agatgaccgt ggagaaataa taagaactac cagtcagata aaacaagata
     2341 ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagatag aacccccttt
     2401 ttatagcata aaaaagattt agaaggatat atgatcctat tgcatgagaa gtacaataaa
     2461 attaataaga agtaacatta acgaaaaaac gtggaaatta ttcagtgaat acaaagaaat
     2521 taaatcttga aaagtaatgg agtatggctt ggaaaagttc aaaaaatatc aagacaagct
     2581 tctcaaaaaa agggattttg atatccaata aaaagagtga atcattgtac tgccctccaa
     2641 aaaagtagta ccaaaaatct aaattttttg gaggatttta catgactcac tcttttttta
     2701 tcaaagaaag tattttccaa gcagtttttt catccaaaaa taaatctgta aaaaaattac
     2761 cattcaaggc gcctaaacaa ctctccgctt tgaacttact tttacaaata ccaattcgtt
     2821 ttggaatatc gaaaatttct tttaaagaaa gaccaaaagt cttatcctca gatgctttga
     2881 tgaattcacc attgatattg aagggtcttc cgtaaagaaa tccaacagta tccgtcatgc
     2941 ttggaaatag gattttacga tgcttgttcc agggttcgac agaatcaata gaggataagg
     3001 tgccaattcc agaaattaac atatctgcat gcgaagcggt tgctaaggta ttagctaaag
     3061 aaggttctag agccattagt tcacgtgcag tttcattgat gacatataaa ggaatactga
     3121 gagtcaaata agaagactca taacgttcag cagctttttg aactaatcgt gtggagccag
     3181 ctaaggaatt atattttccg atttcgccaa tgaattgagt aaaaattaat tcttcacgag
     3241 cacccatttt aaactgatca atcactttgt aaacggtatc tccccaagat agtgcaacca
     3301 cattggcttt cttaattaat tcttgaatgt aatcggcagc aaattttgaa aataataaat
     3361 cgtgttgtga taagtcttca ctacttttta atatatagat attcgtttta aagtaatttt
     3421 tgaagatttc ttctagctca taattcctag catacggaga attaattgaa atactaacta
     3481 attgtttttc acgtgcatct tcaatatatt ttaaagcttt atatctggtg atcccatatt
     3541 ttttagaaat ttcactaata ttgatatcct cgagataata gtcttcacaa atttttacaa
     3601 ggagtgattc atagtcagag aattccagat gcatagtata gctcctaact ttttcaaata
     3661 gatatttttt ctcattatac caaaaaaatt gaatttagtc tttttttaaa tttttaaaaa
     3721 taagcatcat ttttttaaaa tttaaaatga aagggttttt ttattttaaa aaaagtgtta
     3781 aagtggtttt gtaattaaaa aatgtaaaaa ggagtgaagg tatgcttgat gaagttttag
     3841 aaatccataa aaaaaatgta ggtgttttac ggatggaaac aacagaagcc gtgcaagatg
     3901 atcaagattt agcaaaaatg tacaccccag gtgtagcaga actaagttta atgattgaga
     3961 aaaatcatgc tttggcaaga gaattaacaa ttagtggtaa attagtggcc gttattacag
     4021 acgggtcggc ggtattaggt cttggaaatg taggaaacca agctggatta ccaatcgtcg
     4081 aagggaaagc gctgctttat aaaaatttag caggtgtcaa tgcgattcca ttagccatcg
     4141 aacaaaaatc agtagacgag attgtacaaa caattgtcaa tttgcaaaat agttttgcag
     4201 ggatccattt agaagatatt gcagcaccga agtgttttga gattgaagaa aaattacaag
     4261 aaaaactttc tattcctgtg tatcatgacg atcaagaagg aacagcgata gttgttttag
     4321 ctggattgat caatgctgct aagatccaaa ggaaaacatt aactgaactt cgagttttga
     4381 tcaacggcat gggagcatct ggggttgcaa cagctagact attaatcgct gctggtatca
     4441 aaaatctaac attggtggat aaacaaggga gattaaagaa gcaagatagt acattaaata
     4501 aatatcaaag agagttgttg gatcatatac atgatacaaa taataatgca agcgttttgg
     4561 aagatgcaat cgttggacaa gatgttttta tcggtctttc aataggaaat gttttaacgc
     4621 ctgaaatgat tggaaaaatg aatgataatc cgattatttt tgctttagcc aatccagtac
     4681 cagagatcac acccgattta gcaaaagaat ctggtgcaaa gctaatcgct acaggttctt
     4741 cgaaatatcc taaccaagta aacaatattt tagtatttcc cggattgttc aagggactat
     4801 tactatcgaa agcgaataaa gtcgatcttt cattacaact tgaagtagcc aaacaattag
     4861 ctgctaaggt cgcagagcct acggtagaaa aattcatccc taatgttttt gatgaaggag
     4921 tcgcaaacgt cgtttcacag gcagttttgg attatttaca aaaactaaaa gaaaacgagg
     4981 ataatataca tgctgaaaac attaaataga attaaaattg gttttattcc tttatctctt
     5041 tatatcattt tggtactcgc gtatcttgca atgacgataa gcaatatcgt tccagaaaat
     5101 gatatgttag tttctttggg tattatgact gtttttgctt atttattaga agaaattggt
     5161 aaaactattc ctattttaaa agatttaggt ggtaaagtat tagttgttac atttttgcct
     5221 gcatttctag tctataaaca ttggctacct caaagtactg taactgtggt ttctgatttt
     5281 atgaataata acaatttctt gatgttcttt atcacactac tagttgtcgg cagtattgct
     5341 tcaatgaatc ggcaaacatt ggttaaagca acttcacgta ttatcattat tttaatttta
     5401 tgcgatgtga ttggttcttt gataggaaca ggtgtgggaa tactccttgg gctatctgct
     5461 tggaatgcgt atttctttat cgttgcgccg attatggctg gtggtgttgg ggaaggagcg
     5521 atgccattat cgattggtta tgcagcaata ttatctggta tgggacaaaa tgatatcttt
     5581 gggaagattc taccgtgtgt gatgttggga agtttagtcg cagttctact gtctggtatg
     5641 ctcaaaaaaa ttggagagaa atacccacaa ttaaatggtc agggtaattt gatcgatggg
     5701 gatgatgaac atttagagaa cttgaagggt tcagctaaac cagtcaatat tgaaaaaaca
     5761 ttgttagcag gcgtggtagc aataacgatt tattttgttg ctgtctatgt tgagcactta
     5821 ttaaaatcac tccttggctt ttctattcct tcacctgtga tcttattagt ctctcttatg
     5881 ggggctaaaa tgcttggttt tatccctaaa aatatcgaag aaggtggaaa ttcgttgtat
     5941 ggattcactg ttcgtgggat cacacctccc ttactttttg gcgtaggggt tgcaaaaaca
     6001 gattggttag agctgatcga agtatttaaa aatcctgcaa cgatagtaac aatcattgtg
     6061 acggtagtag ctattgtcgc tacagctttt atcgccgcaa aatttttaaa aatgtaccca
     6121 gttgatactg ccatcgcagt ttcatgttgt agcggacaag gcggtacagg tgctttagct
     6181 atcttagctg cgggtgatcg catggaatta atgccatttg cacaagttgc tgttcgacta
     6241 gggggtgcta tgaccgtaac gttcgctatc tttttaatgg gtctgctatc ataagaacat
     6301 aaagaaagga cgtgaccatg tgtttgatca aacagatatc caacgattat ttttaaaaaa
     6361 taactatatc aaagaccaat gggaaaatct tttgcttgat tcaggaatac aaaagaagca
     6421 aatcgaagat tttcaacatt tagatcaaac attgggtata taccataaag aaaagttagt
     6481 aggaacagta tcttatcaaa acaacgtgat taaatatatc gccgtgtctg aaaaatataa
     6541 agatggtgga catacattta atgtactagt ctctgcaata attcaggcaa tgagtcaaca
     6601 agagattttt cattatattg ttttcacgaa accgcgctat caaaaaagtt ttaacttttt
     6661 aggttttgaa acaatcgtat ctaccccaaa aatagctgta ttagaaatgg gagatagttc
     6721 tataaaacag tacctagaga ggattcctaa agtgaatggt gaaaaaaaag cagcaattgt
     6781 catgaatgcg aacccattta cagaagggca ttattattta gtaaaacaag ctgccaataa
     6841 caacgattgg gtatatgtat ttgtactcga aaaagaacaa aatctttttt cgacgaagga
     6901 ccgactcaaa ttagttcaac aaggattaca aaaactgaaa aatgtcattg ttctatcagg
     6961 tggcgagtat attattagcc ctgcaacgtt tcccacctat tttttacgag aagatgatga
     7021 aatagcaaag gagcaaatgg tcgtagatgc tactttattc aaagaaagaa ttgccactta
     7081 tttagatatc tctgctagat atgtgggaga agaaccactt tccccaatga ctcgttcata
     7141 taacgacata ctggaaacga ttttaccccc tgaaataaaa gttcatgtcc ttgcaaggaa
     7201 gaaaacggaa caacaccaag ttatttctgc ttctcaagtt cgtaaagcgt atcttgctgg
     7261 gcaattggaa aaaataaaat atatggttcc agaaacaacc tatcaatact taaaaaataa
     7321 aagggagcgt taagtaatgg aaataaataa atcagcgatt gctggaacat tagagtcaag
     7381 tgatgtccaa atcatgattt caccgaatac agaaggaata gaaatcgatt tagacagtca
     7441 agtgattgag caatttggaa aacaaatcaa aaaagttatc tatgacacac tagcagttta
     7501 tcagattaaa aacgtaaaac tcaaggtagt agataaaggg gcattagatt gcgttattgt
     7561 tgcacgatta aaaacagcga tccatcgttc cttaggtatc caacaccaag aaacaccgtg
     7621 ggaggtttaa ttagatgatg aaagaacgtt tgagaagaac aatgatgttt gttccaggaa
     7681 ataatccagc gatgatcaaa gatgcaggta tttatggtgc tgactcgatt atgttcgacc
     7741 tggaagatgc agtgtctatg gcagaaaaag acagcgcaag aatgcttgtg gctgaggcat
     7801 tgaaaacagt ggattatggt gacacagaat taattgttcg aataaatgac ctgcactcag
     7861 aatttggacg tgcagacatt tttgcaatgg ttaaagccgg cgcgcatgta ttacgtttac
     7921 ctaaaacaga aacctctgaa gatataacag aagtagaaaa agtgattata gaagcagaaa
     7981 actatcataa tgttccgata gggcgaacat tgatgatggc agccattgaa ggtgcttctg
     8041 gtgtattgaa cgcacctgcg atcgccaaag catctgatcg attgattgga atcgccattg
     8101 gggcagaaga ctatgtgaca aatatgaaaa ccaaacgata tccagatgga aacgaattgt
     8161 tttttgctcg cagtatgatc gttcatgctg cacgtgcagc aggcatttcc gcatttgaca
     8221 ctgtttactc tgatgtggat gatatggaag gatttttaaa cgaagtacgt ttaatccatc
     8281 aattaggttt tgatggaaaa tcttgtatca atccacggca aattccagta gtcaatactg
     8341 tttatcagcc gtcaatcaaa gaaatcaaac gatcactaga catcatctcg gcaatcgaag
     8401 aagcaaaaga aaaaggttct ggcgtaattt ctttaaatgg aaaaatgatc gataaaccaa
     8461 ttgtagaacg tgcacaacgg acattgatgt tagcaaaagc atcacgattg attgatgagg
     8521 agggaaatat ccatgcataa taaacaaaga cgtagcattc catcaagtgt tttaacaaag
     8581 tataaaaaag aattccaaag tgtttcatta tcgccacaaa cgattcaacg ttgtgcacca
     8641 aaagtaacga ctgtaactaa tcagacaaat aaattgatgc actcgatcaa agaagctata
     8701 gaaaaatcgg gcttaaaaga tggaatgacg atttcatttc atcatcattt gagagaaggt
     8761 gattttgttt ttaaccaagt gatggccgtg atccgtgaaa tgggcatcaa agaattgacc
     8821 ttagcaccaa gttctttaac gaacactatg aatgagatgg ttattgaatg catcaaggac
     8881 ggtacagtaa ctcatatcac aagcagtggg atgcgcggca gcttaggtga atttatttct
     8941 catggaggaa tggacaatcc tgtgatttta cgttctcatg gtaatcgtcc acgtgcaatt
     9001 gaaagtggtg aattgaaaat cgatgttgca tttctaggag taccaacatc tgatgattat
     9061 ggaaatgcta atgcgactgt tggaaaatcc gtttttggtt cattagggta tgcaaaagta
     9121 gatgcacaat atgcagataa agtcattctt ctaactgatc atttagttcc ttaccctaat
     9181 acgcccatta gtattccgca aacacaagtc gactatgtcg tggaagtaga agcaattggt
     9241 gatccgaata aaatcggtgc tggtgctaca cggtttacta aaaacccaaa agaattaaaa
     9301 attgctgaaa tggtcaaaga tgtcattgtt cactcacctt atttcaaaga tggcttttct
     9361 tttcaaacag gaacgggtgg tgcagcttta gcagtaacgc gttacttagc tagtgaaatg
     9421 gaagaaaaac agatcaaagc ttcttttgca ttaggtggca tcactaaacc aacggttgat
     9481 ttattggaaa aaggtcttgt tgaaaaagtg ttagatgtcc aagactttga caaaggagca
     9541 gctgaaggaa tggcgcggct tgagaaccaa caggagatcg atgcttcctt ttatgctgat
     9601 ccaagtaata aaggagcggt tgttaaccaa ttagatgtcg ctattttatc tgcattagaa
     9661 attgacacaa acttcaatgt aaatgttatg actggttcag atggtgtgct aagaggtgcg
     9721 attggtggac atcaagatgc agcaacggct aaacttacta tcatctcagc gccgttagta
     9781 cgtggccgaa tacctacagt agtaaatgat gttactacat tgatcacacc agggaaaagt
     9841 atcgatgtat tagtcacaga agtaggggtt gctatcaatc cccaacgtaa agatctaata
     9901 gctatttttg aaagaattcc acaaattcct gtttttacaa tagaagaatt acagcaaaaa
     9961 gcagaaaaaa tagtaggcat cccggaacct ttgcaattca cagatcggat agttgcctat
    10021 gtagaatacc gagatggttc aattttagat gtcgttcgtc aggtgaaaga atttgaagaa
    10081 gagagatcat gatgtttgat actggagaaa ttcaagaaat cgaggatatt ttgatccata
    10141 aagaacaacg tgctaacttg caaagcaacc tgctttccaa gtattcaaat agttcattga
    10201 tggtctttac attgaatatt cctgggccga tcaaaaataa tccaatgatt gagcaggtat
    10261 ttcgtcaggg acagcagtca attcagcaaa tgtttttaaa aaaaaaggtg gaaatgacgt
    10321 acacaaaaga aatcaatttg ccaagtggat tggactggta cgtaatagta acagaagcac
    10381 ctttgaatct caaaaaatgg ttggtcacgt tagaagagca agacgcatta ggtagacttt
    10441 ttgatttaga tgtcttgttc aaaaaaggag agcagataac aattgtcagt cgccaagctc
    10501 ttaatcatgg ggaacgtcct tgttttattt gtgggaaatc agcaaaggta tgtgcacgta
    10561 atcgtacaca tactgtgaaa gaaatgcaaa agaaaatcga acaaatagta gccgattcct
    10621 taaaaggagc attgaaacat gcatgataat attcaaaaaa tagcacaaga agcacaatta
    10681 gcgatgcttt atgaagttac atgtatcaat aaaccaggat tggttgatcc agtagacccc
    10741 gggtctcatc aagatatgga catttttact tttctacaaa gtagtgtagt tttgactccc
    10801 tattttgaag aatttatcca aacaggattg gaccgacaac atcaaccaat tgaagaaacc
    10861 tttatggcga ttcgacagat tggtttagag gctgaaactg cgatgttctc agtgacaaat
    10921 ggtataaata cacataaagg tgctatcttt tcattgggga tttttcttgc tatttgtggc
    10981 cggttaacta tttggaaagt tccttgtaaa aaaagtttct ttcaaaaaga aattcaaaaa
    11041 atgacgaaaa atctacttaa cgattttggg aagattgatc aaacaaaacc aaaagcatgg
    11101 acttggggag aatatctatt tgttaaccat ggattgacag ggatacgagg ggaagccaaa
    11161 agaggttatc cttgtgtttt tgaccgagga ttgccttatt atcaagcata tcaagggtcg
    11221 caacaagata agatgataga cactttactt tttctatcaa tggaagtaga agataccaat
    11281 ttgatcaaac gaagtaataa tagacatgtt tttgaagagt atcaattact tattcgtcct
    11341 tattttgaat tagggggtgt aaaaaccata caaggtaaaa aatatttgga ttacctaaat
    11401 cagcaattta aaaagaaaaa ttggagtatt ggtggaagtg cagacttatt gatccttaca
    11461 atctttttag ataaaatcat ggacaaagga tggttatgtt gatactataa tacattattt
    11521 tgtattagta agctaataac taaaaaatgg ttcaatacaa atattttata aaaaatggta
    11581 gtgcctatcc tttcagattg tagacaaaga aaaatttctt tgtctacaat ttttttgtta
    11641 atgacttcag tcagtttcgt gcgcaaagcg tgcgaaacaa gaaaaccatg cgtttctaca
    11701 cgtggaagaa ctagcatatc gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag
    11761 cccatactcg ttggaatttt aagtatcata ttgtatttac accaaaatat cgacgaaaag
    11821 taatctatag gcaattacga aaagatattg gaaaaatact taggaagttg tgcaaaataa
    11881 aaaacgtgga aattatagaa gcacatgcaa cgccagatca tattcacatg cttgttcaag
    11941 taccatcaaa aataagtata tccagtttta tagttatctg aaagatcgta gtgctgtgct
    12001 aatccacgag cagcacgtaa atcacaaata tagatacgga aataaatcat tttggtcgga
    12061 ggaatattat gtgagtacag tcgaattgaa tcaaaagaca attgaaaaat acatccgtga
    12121 acaagaagct gatgatcgca tttgagatag tataagtaaa agagagtaga tgttgcaata
    12181 acaaaagggt aaaaatggac tacctccagt aaaataccga aaaatagttt aacttttgga
    12241 gataagtcca tttaagggtc tatagaatga caattttttt aggggatttt actattatta
    12301 cttcaagacc cgcttggatt cgcaaagtga gtgaactctt aaaactacct gatagtaggt
    12361 tgttttaaga gttatctgaa agtaaaaatt gcatttatat ttttgataaa tacgtaaatt
    12421 tatatatacg tatttatcaa agttttaggc caaaggaata ttgtgtaaat acactaggat
    12481 tgaatgaata gaccagaaga aattgcgtcc aagaacaaaa gaagcatgat agtgctctat
    12541 ataaattggg cattcaagaa tctgaaattt aaaggtagaa ttttagttag acttttagat
    12601 tttaaacaca aaaataacca tacaagcaac aagaagcaaa gtgctatatt ataaattgtc
    12661 cgtccgttta cttacataag atcattgggg ctatttttaa aagagaataa tagataataa
    12721 atgaaaaaaa agtcaagtcc acaatcctgt gtaaaatact atacaatgct ctcactattt
    12781 tttacttggc aaatttaata tattttcttg atgaagaaag ccactcatcg tgttgatcaa
    12841 taaggactgc tccaattaaa cgattggctg aaacgcagtt tggaaagata cgaataattt
    12901 tttctcttct gcgcacttct tgattcagtc gttcaattag attggtactc tttagtcgat
    12961 tgtgggaatt tccttgtacg gtatattgaa aggcgtcttc gaatccatca tccaatgatg
    13021 cgcaagcttt tgaatatttt ggttgatcga tataatcatg aatcaatcga tttttagcct
    13081 cacgagctaa gttaatatct gtgaacttaa aaattccttt aacagcttct ctgaaagatt
    13141 ttgaattttt tttaggaatg gtggtaaaga tatttcttag gaagtgaact tggcatcttt
    13201 gccaacttgc gttggtgaag gattttctaa tggcagagac taatcctttg tgcgcatcag
    13261 aaataacgag ttctgtacct tgtaaaccgc gttcttttag gtattcaaaa aatgttatcc
    13321 aggtctcttc gctttcgcca ctttgaatca tgaagccgat aatttcacgg tcgccatctt
    13381 tggttattcc aatcgctata tgacagcttt ttgagagtac tcgattttct tctcgtactt
    13441 ttatatagag tacatcggtc attaagtaag gataattttt ttctgataat aaacgattct
    13501 gccactcgtt aaccattggt tctagctgtt ctgttaagct agaaacgaag gacttagaga
    13561 cggatttacc acaaagttct tccacaattt ttgatacttt acgagttgaa acgcctgata
    13621 catacatttc caacattgaa gcaatgaggg ctttttcgtt tcgttgataa cgttcaaaca
    13681 ctgtgggtga aaaatggcca tcacgtgttc tgggtacttt taattctagc gtgcctatac
    13741 gtgtcgtaaa gctgcgctca taatagccat ttcgttgact ttgtcggttt tctgttcgtt
    13801 catattcttt tgcttgaata tattctgttc gttgattttc cattagttga ttaaataccg
    13861 ttgttaaaat atttttagaa acgtcatcct ttacagaata ttcaataatg ctttgaatct
    13921 cttcgctttt cagtgtaaaa tgtacttggg tcatgtaaaa gtcctcctgg gtatgttttt
    13981 gtcgttaaaa acattgtacc gtaaaaggac tgttacatgg cctttttact tttacacaat
    14041 tataaggact ttatcgaaaa aaagcagtta tttgttaaaa tcattgatat tattaataat
    14101 aagtattttt tgaaagggga aatataaaag gtttttgaaa ataaataaaa aatagtatta
    14161 cttgttctat tgctagttat tgctagtctt tattcttttt tgttttttag ggatgtacaa
    14221 aagatctcaa ccacaggaga cattgctttt cacttttcta gaataaaatg cttgtcctca
    14281 gtattttctg gtccaataaa ttttaccaca tttaatagct atggcggagg cgtgaatttt
    14341 tttatccata tctaacatct ttccagccgt aatattttac tggacaacaa acaatttgat
    14401 tctaagttat atacttacta tacgttggga tactgaatat ctgtacaatt ctagtgagct
    14461 atcaatatgg acaaaagaat agatgcagcc ttcttattca gctgtttgta tagcttttat
    14521 ggattgatat atattagcgt actgcaattt cagaatcaat tgcgttgacg attgtaccag
    14581 ttgtcttgta ttacacatat actcttcttt atgaaagaaa aaactgcgtg attcttttag
    14641 ctgtcagtat gagtttgctc atctataccc atattctctc cacctttatg agtgtgattg
    14701 ttattggctt cttgtttatc ggctctcttt tctctaagcg gaaagagaga aaagaaatgt
    14761 cgaatctgat atcaaatatg ataaaagctg ttggttggac cattctttta acagcttacg
    14821 tttggtatcc gatgttacaa caagtcctac atcaaaaaat taatcgtcct tttcgcacaa
    14881 aacttcaaga aagggcatta aacatttctg atagtttaat cggtgcagta aataatgatt
    14941 tagttacatt tacaatggga acaattggtg tggtttcatt tattcttcca cttttcttta
    15001 aaaaaaaatt tgccaataaa gaaaagatta ttaatttttg tgcagtgacc acttggttct
    15061 tatcaacgaa tctttttcca tgggaatttt tacaaaaaac acctattcaa atcattcagt
    15121 ttccttggag aatcttgggt tttcaagtgc tttttggttc attgatctta attatagtgt
    15181 ttttaaagtg gaaaacaagc aacaaaaaaa gtatgtgtag ccttgtagga atagtccttc
    15241 ttatttttac agtgactgta gcaacagaag cgaattattc gcagaaaatt caatcgtata
    15301 aaggtcgttt aattatgacc aaaaaagatg ttacacttta tacaacctcg agaactggag
    15361 gtctttatga ctatgcacca ttagatgcat tgaaatataa agatcattta aagaaacatg
    15421 aagtaaaagt ctatgattga tgggagaatg catctcataa agcacataat tctaaaataa
    15481 tctatacagt aggagatgcg aatgagcaaa atatcacgtt acctgtattt aattactggg
    15541 aacagttatg aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc
    15601 tattaaaggt acgggaaaaa gaactatagt tgaggtttca tctacgtatc caactacgat
    15661 gattctatct ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat
    15721 aagatgctgc ggttaaatga aaatagatta gatagatatt ataaatatgt tgattctatt
    15781 ttcatagcaa tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacggt
    15841 gaatttattc cgaaaagtga aaagaacaaa tataaaattt aataaaaata tagaactaaa
    15901 taggaaaacg tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga
    15961 aatttatata aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga
    16021 taatttttag gtctatttaa ctcatcagac attctatgct cattttctcc aaatttgttt
    16081 caactctttc gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta
    16141 cgatactagt atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat
    16201 agcattccta aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta
    16261 cttgaacatg aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt
    16321 gcgcaacatt tattgattgc aaaattatgt gacaaaaaga actagtagaa gaaggaataa
    16381 tggcttgata ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt
    16441 aacatactac tatattttaa gtttcatatt atataaatta ataatatact accatttttt
    16501 ggtagtataa tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca
    16561 aggaaagttt aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta
    16621 ttatctttag tgatataacg aaatatctac acagacaaaa
//
LOCUS       DAFBYI010000060        15849 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFBYI010000060 DAFBYI010000000
VERSION     DAFBYI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.060"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(290..490)
                     /locus_tag="LLD04_001718"
     CDS             complement(290..490)
                     /locus_tag="LLD04_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5648648.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(795..2027)
                     /locus_tag="LLD04_001719"
     CDS             complement(795..2027)
                     /locus_tag="LLD04_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK5648649.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2284..2853
                     /locus_tag="LLD04_001720"
     CDS             2284..2853
                     /locus_tag="LLD04_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5648650.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3031..3471)
                     /locus_tag="LLD04_001721"
     CDS             complement(3031..3471)
                     /locus_tag="LLD04_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK5648651.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3629..4393
                     /gene="map"
                     /locus_tag="LLD04_001722"
     CDS             3629..4393
                     /gene="map"
                     /locus_tag="LLD04_001722"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK5648652.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4425..5348
                     /locus_tag="LLD04_001723"
     CDS             4425..5348
                     /locus_tag="LLD04_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK5648653.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5424..6563
                     /locus_tag="LLD04_001724"
     CDS             5424..6563
                     /locus_tag="LLD04_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK5648654.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6556..7356
                     /locus_tag="LLD04_001725"
     CDS             6556..7356
                     /locus_tag="LLD04_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5648655.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7356..8183
                     /locus_tag="LLD04_001726"
     CDS             7356..8183
                     /locus_tag="LLD04_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5648656.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8161..8895
                     /locus_tag="LLD04_001727"
     CDS             8161..8895
                     /locus_tag="LLD04_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5648657.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8995..9861
                     /gene="rfbA"
                     /locus_tag="LLD04_001728"
     CDS             8995..9861
                     /gene="rfbA"
                     /locus_tag="LLD04_001728"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK5648658.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9875..10447
                     /gene="rfbC"
                     /locus_tag="LLD04_001729"
     CDS             9875..10447
                     /gene="rfbC"
                     /locus_tag="LLD04_001729"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK5648659.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10469..11497
                     /gene="rfbB"
                     /locus_tag="LLD04_001730"
     CDS             10469..11497
                     /gene="rfbB"
                     /locus_tag="LLD04_001730"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK5648660.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11595..12446
                     /gene="rfbD"
                     /locus_tag="LLD04_001731"
     CDS             11595..12446
                     /gene="rfbD"
                     /locus_tag="LLD04_001731"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK5648661.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12480..14513
                     /locus_tag="LLD04_001732"
     CDS             12480..14513
                     /locus_tag="LLD04_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648662.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14557..15837
                     /locus_tag="LLD04_001733"
     CDS             14557..15837
                     /locus_tag="LLD04_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK5648663.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4868 a         2735 c         3285 g         4961 t
ORIGIN      
        1 tcaaatttaa accataattg tgtttttcct tccatcagca taaaagttca cctcaaaaag
       61 agtatacgaa cgaatgttct ttttgtgaag gcggaattta atatactgaa taatagaaaa
      121 caagtacttg tgccaaatta tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat
      181 agaaaacctt ctatattaag atttcctgca ataacaaaca caacagaaga cgtgtaatag
      241 ttagtcagga acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat
      301 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
      361 ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccctca ataaacagtc
      421 gaagagaaaa taggcaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt
      481 tgattttcat acttcaacag attcgaatta aacgatttcg attggcttat accagtccta
      541 tccgacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc
      601 caatgggaat gaactggaga tgacttttta ttatttttag taatagctga tgggttaata
      661 cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata
      721 aaggatctaa gccaatagct tagatccttt attctatgct ttatattatt ttttgcatgt
      781 gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa tcggtactgt agagccatct
      841 tcacggattc cgtcgatatc catcttgtca gatcctacca tgaaatctac gtgggtttga
      901 cttctattca atccagcttc tgctaattct tcatcggaca tttctgtacc gcctttcaca
      961 ctgaaggcat aagcagaacc tagtgctaag tgattcgatg cgttttcatc aaataatgta
     1021 ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt caggtacaag tgcgacttct
     1081 cctaaacgac gcgcaccttc atctgtatca agcagttttg ctaagacatc ttctccttgt
     1141 tcggcagaaa aatcaatgac ttttccatct ttaaaagtaa atttcatacc cgagatgatg
     1201 gtgccagcat aactcagtgg ctttgtactt gaaatgtaac catctactcg atgactgtca
     1261 ggtgctgtga acacttcttc cgtcggcata tttgccatga atttttctcc acgagcatta
     1321 tagcttccag cgccttccca aagatggttt ttaggcaatc cgataatgat atctgtaccg
     1381 ggagcagtat agtgcagggc agaaaattgt tctcgattca attcttctgc tttttttgct
     1441 aatttttcat catgcttctt ccatgctaag acagggtctt cttcatagac acgagtcgtt
     1501 ttgaaaatct gatcccataa tgcctcaact tgtttttctt ctggcagttc tgggaagact
     1561 tttgctgccc attgtttacc agccgcagcc acgacagtcc aactgacttt atttgcttga
     1621 gttgccttgc gtaaattcat tagtgctttg ccagccgctg attgataaga cgctactcga
     1681 tggctatcta caccagcaaa tgcatcggga tcggcagaaa caacactgat cctgcttgct
     1741 cctttttcaa gccattcatc cgcttgatcg attttagatt gtggtacatt ctcaatacga
     1801 tccgttgcag catgtaaaag aaattctctt tggatctggt cgtcagtcca ttgaacgata
     1861 acctcagcgg ctcctagttt ataagcttct tgtgtgatca atcgagcaag gggtgcctgt
     1921 tccacgctga tctgtaaaac aactgtgtgt cctttttctg tggctacgcc agtttctgca
     1981 attaagcgtg catatttttt tagaagttca ttaaaatcag ataacataat aaaaattcct
     2041 cctcaccatt ttttctggta actattagca tgataccatt taattatgat tcaaacaatg
     2101 aggcctttaa ataaaaaaaa gaggttattt ttttaaggtt aagtttcatg atatatgtta
     2161 ctacttgcta tttcagcttg tgaagaaaat caaaaactaa ttttgcatat caaaaaaaac
     2221 atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg agattaaaaa
     2281 aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc ttatgaagta
     2341 gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa aatgaattgt
     2401 tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca agctcttttt
     2461 gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac agggaatacg
     2521 attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa gttgtatcgt
     2581 tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc ttatacttac
     2641 tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga gcagatcgaa
     2701 tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat gtcttcaagc
     2761 atcatccatc caactgatga acagattgaa aagatgatac aagacagtat agatgctatc
     2821 ttgaaattca aagaaccaat agaaatcgat tgaatatact caaaagttcc acaaatcagt
     2881 agataacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc gtatgcattt
     2941 ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccgtac agattgaata
     3001 aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt ctttagcgaa
     3061 agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa cattttcggc
     3121 tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa
     3181 ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttcccg atagatccaa
     3241 ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat catcgtatgt
     3301 ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta cttgggtaca
     3361 ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa tatcggcgat
     3421 ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca tgatgtgcct
     3481 cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga aaagaaaata
     3541 aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat gttaaaatga
     3601 gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac gtgaaattga
     3661 aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc gtgattttat
     3721 aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata tcgagagtca
     3781 tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt gtatcagtat
     3841 aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg gtgatttggt
     3901 aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt gggcctatgt
     3961 agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga aagctttgta
     4021 catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc atgctatcca
     4081 aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc atggcgttgg
     4141 tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa aagggttgcg
     4201 gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa cttggaaaat
     4261 gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt cttgccaata
     4321 tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac aaggagaaga
     4381 aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa gtttggaaca
     4441 aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag cgcatcactg
     4501 attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg tctttatttc
     4561 cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa gagactgtac
     4621 tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt cctgcaatca
     4681 aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta gcggctttat
     4741 ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg tatggcgtag
     4801 aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt gtcttgttca
     4861 tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt ttggatgcta
     4921 ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact ttgaagtggc
     4981 caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt gtccctaatg
     5041 ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc ggctggatgc
     5101 tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc agcggttatc
     5161 agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct acgatcatca
     5221 ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat caaatcaaaa
     5281 aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt tctcataaaa
     5341 aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttataa taattatgac
     5401 tcttaattaa agtaggtgcc catatgcttg tttcttttag tatggttata agattttttc
     5461 tgacaatttt attatcactg atattaacac ctgtattcaa aatcatttca gtacaaacag
     5521 gtatggtcga caaaccaaat gaaagaagaa taaataaagt tccaatgcct tcagcgggcg
     5581 gactgcctat ctttatttca tttgttatat cgactttatt cttattccgt aaaattatcc
     5641 ctcaatcgta tattgtaccg atcttgattg cttctttagt aatcatagtc acaggagtgc
     5701 tcgatgataa atatgagctg acacctaaac aaaaaagtat agggatcttg attgcttctc
     5761 ttattattta ttttgtagca gatatacgta ttgattcctt cacgcttcct tttattggat
     5821 atatccaact aggctggctt agttttcccg taacgatttt ttggatcttc ggtatcacga
     5881 atgcggtcaa tctgattgac ggtttggatg ggctggcggc agggatttca ttgatcggtt
     5941 taattacgat aggaatcatt ggatattttt tcctccatgc ttctacagtg tatattccag
     6001 ttgttatttt ctgtcttgta gcaagtatta tcggtttttt tccttacaat ttttatccgg
     6061 caaagattta tttaggcgat acaggagctc tattcctagg gtttatgatg gccgtccttt
     6121 cactacaagg gctgaagaac gtgacttttg tttcatcgat ttctttgctg atcgtcatgg
     6181 gagtgccggt aacagacacg ttttttgcta tcattaggag aaaagcaaac cgtgtttctt
     6241 tttctacagc agataaaaaa catcttcatc accgactaat cagtttaggc tttactcata
     6301 aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca gctatggtca
     6361 tgaattatac tggaagaatc ggaacgatca tattgattat tgccatgttg tttgctgcaa
     6421 ttttattatt tgaattgatt ggtctgatca atgagaaaca tcagtttatt ttaaaaacat
     6481 tgcgattttt agggaataaa gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat
     6541 ggaaccgtca tatagatgaa taacaaatta aaccagccga tccttattag tattgtgacg
     6601 gcaaacagtg ataaaatttt tcggacacta gataatttta tttcaactgt aaaagatgaa
     6661 acagcagtaa aaatccgaat ttttgacaac aattcacttc ccgagtacaa aaaaagacta
     6721 acagagtata ggaaatatcc ctacatcgat atctattttc atgatgaaaa ttccggattt
     6781 ggttatggac ataatcacaa tctgatagct agcgattctc gctatggcat tatttgtaac
     6841 ccagatattt tagtggatga agagacagtt acagcattgg tcttgttatt gaaagaacat
     6901 ccggaatgtg cgatgttagc acctaagatt cttaatgaag atggaacaac tcagcacctc
     6961 atccgtgagc agctggctgt ttttgattac gttttgcgct ttgtgccttt caaatgggtc
     7021 aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc gaggattgcc tactgatgct
     7081 aatagttatg tacggatgat ctcaggtagt tttatggtag ctgatattga taaatttaaa
     7141 gagataaagg ggtttgatga gcgtttcttc atgtactttg aagacaacga cttgtgtttg
     7201 agttttgaaa aagcaggtta caagttattg tacacaccct tttatgcagt tatccatttt
     7261 tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg
     7321 aaatttttca ataagtgggg gtggcgtttt ttctgatgaa accagaaata ttagtagtcg
     7381 ttgtgcttta tcagcaacta ttcagtcaat ctccagctta tggaccatta agcaaagcac
     7441 ttagcgaaaa gcaaattcag cttattattt acgataatag tcctcaaagg cagcaaaatg
     7501 agctgtttga aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg
     7561 tggcttataa ttttgcgttg tctcaagcag atgaaggaac acgttgtctc gtcactctgg
     7621 atcaggatac gaggctagtc gacgattatt ttgaactatt gcgtaaagta acctttacag
     7681 atgaatgtgt tgctgcagtt ccgatgattt tttctgggag ccgacaagtt tctcccctat
     7741 attcagatcg ttatatcaat cgtacagctc aagcagtaga agttgaaggc ccaacagctg
     7801 aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt caaatttcta aaaaagattg
     7861 gcgggtttaa tcctgctttt tctttagatt ttttggatca ttggctgttt tggaaagtgg
     7921 atcagttgga aaaaactgtg gagatccttc ctgtacgaat ggaacatgat ctttcggttt
     7981 tagactatga aaaagtttca agtaatcgat accgttctat tttacaagca gaaaatcgat
     8041 tttatcatga atatgatcaa aggcatttat ccagtcaccg acgacaatta ttgttaagaa
     8101 cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat
     8161 atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt gtatagctac ctttaatgga
     8221 ggcagatatt taagagagca gttagatagt attttatctc agctttcagc agaagatgaa
     8281 gtagtcattt ctgatgatgg gtcagtagat gatacatggg atatattaac tgaatacgca
     8341 gaaaaagata agcgaatcca gcttttttcg gggcctcgtc aaggattgat cgctaatttt
     8401 ggttatgcga tccagcagac gaaaggagat ctaatctttt tggcagacca agatgatgtt
     8461 tggttggaga caaaagtgaa aaagatcact gactattttg agcagaatcc ggatcagcta
     8521 gtagtagttt ctgatttaat cattgtggac agcgagctgc aagagatcca tgattcgtat
     8581 ttttcgtttc gaaattccaa agaaggatgg cttaataatg tgataagaag tcactatatt
     8641 ggtgctggta tggctttccg gtcttcttta aaacccgata ttttgccgat cccgtcagaa
     8701 gtgccgatgc atgatatgtg gattggtttg ctgggaggaa agaagactgg gttcctaaga
     8761 gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga gtgagattga aacacattct
     8821 aaattacggc aaaaaatttc ttggcgtgtt catctgatta cggcgttgat caaacgaaaa
     8881 tggtttaaac gttgataaaa gatgtttaaa tccttaatat ttccatgaca aatataacaa
     8941 ttgatgagat aataacaaag tgaaatttga aatcaaaaag gagagcagat atccatgaag
     9001 ggaatcattc ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg
     9061 aaacaattga tgccgattta tgacaaacca atgatttatt atccaatgtc tactctgatg
     9121 ttggcgggaa tcaaagaaat tctgattatt tcaacgccac aagatacacc acgtttcaag
     9181 gaattatttg gaaacggaaa tgatttagga ctgcagatcg aatatgcagt acaagaaagt
     9241 ccagatggat tggcacaagc gtttatcatt ggagaagaat ttattggaga tgatagtgtc
     9301 tgcttggtat taggggacaa tatttactat ggtggcggtc tgtctaaaat gttgcaacga
     9361 gcagcttcaa aagatacagg cgcgacagta tttggctatc atgtcaatga tccagaacgc
     9421 tttggtgtag tagaatttga tgagaatatg caagctttgt cgattgaaga aaagcctgct
     9481 cagccaaaat caaattacgc tgtaactggc ctttattttt atgacaatga ggtaatttct
     9541 atcgctaaag gtatcaaacc ttcagaacgt ggagaactag agattacgga tgtcaataaa
     9601 gcatatttgg ataaaggaaa actatccgta gaacttatgg gacgcggatt tgcttggctt
     9661 gatacaggaa cacatgaatc attgcttgaa gcatctacct ttatcgaaac aattgaaaaa
     9721 cgtcagaact taaaagtggc ttgtttggaa gaaattgctt accgtatggg ctacatcgat
     9781 aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta
     9841 ttgaatttgg ccgctgaata aggagtcctt acagatgaaa gtaacagata caaaattaac
     9901 agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat cgcggtttct tcacagaaag
     9961 ttattcaaaa gcaaaatttg cagaacatgg attgaaatat gactttgttc aagacaacca
    10021 ttcactatca gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc
    10081 acagacaaaa ttgattcggg tagtcactgg agcagtttta gatgtagtag tggatatccg
    10141 caaaggtagc ccgacttatg gtcaatggga cggatttatc ttatctgaac ataatcatcg
    10201 acagttgctt gtacctaaag gatatgccca tggattcgtt actttgacac caaatgtgaa
    10261 cttcctttat aagtgtgata attactataa tgcagaagca gatgggggca tcgcatttga
    10321 tgatccagat ttagcgattg attggccaat tgatccatca agagcgatta tgtcggaaaa
    10381 agatcaccgt catccgacat taaaagaatt tgaagcagaa aatccatttg tttacggaga
    10441 aatctaagaa ttaaggagaa attttcttat gaaaaacatc attgttaccg gaggtgccgg
    10501 attcatcggc tctaatttcg ttcattacgt ggtaaacaat catccagaag tgcatgtcac
    10561 tgttttggat aaactgactt atgcaggaaa caaagagaac ttggctggac ttcctagcga
    10621 tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa ttagtggacc gtctagtaaa
    10681 agaagcggac gcagttgttc attacgcggc agaatcccat aacgataatt cattaaaaga
    10741 tcctttccca tttgtacaaa cgaatatcat tggtacgtat acacttttag aagcttgccg
    10801 caagtatgat gtacgctatc atcacgtatc tactgacgag gtttacggcg atcttccatt
    10861 acgtgaagac ctgccaggac atggggaagg cgaaggagaa aaattcactg cggaaacacc
    10921 atataatcct tcaagtccat attcttctac aaaagctggt tcagatctgt tagtaaaagc
    10981 atgggtgcgt tcttttggct tacgtgcaac catttcaaac tgttcaaaca actatggtcc
    11041 ttatcagcat attgaaaaat ttattcctcg ccaagtaacg aatatcttaa gcggtattcg
    11101 tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg atccatacga atgatcattc
    11161 ttcagcagtg tggctgattt tgacgaaagg cagaattggc gaaacctatc tgatcggtgc
    11221 agacggtgaa gaagataata agaccgtgat ggaattgatc ttagaaatga tgggtcaacc
    11281 aaaagatgcg tacgaccatg taaacgaccg tgcaggacac gacttgcgtt atgcaatcga
    11341 ttcgacaaaa ttacgggaag agttaggatg gaagcctgag tttacgaact tccgtgatgg
    11401 gttggctgac acgatcaaat ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga
    11461 ggccgttgaa gcagcatatg ccaaaaatgg acaataatac agagaaaatg tcactgtatt
    11521 aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag
    11581 gagcggataa tcatatgttt ttattaactg gaggaaatgg gcaactaggt acagaattgc
    11641 gccatttatt agatgaaaaa aatctgcaat atgtttcaac agatgcaaaa gacttggata
    11701 tcacagatgc cgagaaaaca atggcttaca tcacagaatt aaaacctgat gttattttcc
    11761 attgtgcagc ttacacagca gtagataaag cagaagaaga agcaaaagaa ttagatgaga
    11821 agatcaatgt tgatggtaca agaaatgtag cacttgcagc aaaagctgtg aatgctcaat
    11881 tcgtctatat cagtacagac tatgttttcg atggaaaaaa caaaaatgta gaatacgaag
    11941 tagacgacca aacgaatcca ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag
    12001 cagtagaaga gattttagaa gactattata tcatacgaac ttcttgggtt ttcgggatct
    12061 atggacacaa tttcgtcttc acgatgcaac gcctagcgga aacccgcgac aaactgactg
    12121 tagtaaatga ccagtttggg cgaccaactt ggacacgtac tttggcagaa tttatggtat
    12181 atgtcatcga agaaaaagca ccatttggta tttatcattt gtcgaacgaa aatagctgtt
    12241 cttggtatga attcgcaaaa gaaatattga aagatacaga tgtagaagtt gcacctgtta
    12301 cgtcagaaga atttccgcaa aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa
    12361 agacagaaga gttaggattt gtcataccaa cttggcaaga agcacttgcg caaatgctgg
    12421 aagctgccaa aaaaatgaat aagtaatttc cttttatcaa atagaatttg gagcatgata
    12481 tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt
    12541 cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg
    12601 tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt
    12661 cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaggtgcggt
    12721 cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag gactttccgt
    12781 tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg
    12841 caaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag
    12901 atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg
    12961 aagtactgat gatattaacg aagagaaaaa aagctctatc gctaatcggt gcatttgcgc
    13021 tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct
    13081 ttactttggg actgatgact gctttttatc attatttcta caatcatgat aaaaaatggc
    13141 tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt
    13201 acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta
    13261 tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag
    13321 ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag
    13381 cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg
    13441 gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga
    13501 ataactctga agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat
    13561 ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt
    13621 tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca
    13681 ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt
    13741 tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata
    13801 aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa
    13861 tagataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt
    13921 tagcatttgc attatttggc tggagacgct tgttttacct cgcttttgcc gccttagtta
    13981 tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa
    14041 cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg
    14101 aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct
    14161 tttatccaga tatagctgca tggaaggaaa tcgatccgaa agaagaatac gagaagtatt
    14221 ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag
    14281 atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg
    14341 actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca
    14401 cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt
    14461 atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta
    14521 gctgagaata atcctgaaat attagtagaa aggagagtgg agataattca agcaatcgta
    14581 caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt
    14641 tttgtttgga cagcagggtt aggttttcca gcaactttgt ttatatataa caatagttca
    14701 ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc
    14761 gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg
    14821 gaattaagaa atgagtcttt atttggagat ttactgtttc ctttgattat tttattttgt
    14881 ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt
    14941 acggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga
    15001 caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac gaatacaatc
    15061 ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga
    15121 tggtatgtac gaatcatcgg tgcagtagca gttgttgcag cttttctcgg gatttgggtc
    15181 tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct
    15241 aaatcgttga tgcgtgtcat tcgtgcgcca tttattgctt acacggttac gattctttta
    15301 gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga
    15361 gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca agttttacta
    15421 ggaatgaaaa catttatctt ccaacgagga aaccaatttt taggaaacca caatagttat
    15481 aattctattt tgaatatcta tggattgata ggatttggga ttgctacttt gctgcttatc
    15541 ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc
    15601 tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca
    15661 tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa
    15721 gttgcggagg aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca
    15781 tcttatgggg aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag
    15841 gcacaaaat
//
LOCUS       DAFBYI010000061        15512 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFBYI010000061 DAFBYI010000000
VERSION     DAFBYI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.061"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(330..632)
                     /locus_tag="LLD04_001734"
     CDS             complement(330..632)
                     /locus_tag="LLD04_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648664.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LLD04_001735"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LLD04_001735"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="LLD04_001736"
     CDS             complement(1389..2162)
                     /locus_tag="LLD04_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5648665.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="LLD04_001737"
     CDS             2306..2656
                     /locus_tag="LLD04_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5648666.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="LLD04_001738"
     CDS             2637..3353
                     /locus_tag="LLD04_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648667.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="LLD04_001739"
     CDS             3353..4801
                     /locus_tag="LLD04_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5648668.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="LLD04_001740"
     CDS             complement(4871..5380)
                     /locus_tag="LLD04_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648669.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="LLD04_001741"
     CDS             complement(5370..6095)
                     /locus_tag="LLD04_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648670.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="LLD04_001742"
     CDS             complement(6318..8438)
                     /locus_tag="LLD04_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5648671.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8668..9846
                     /locus_tag="LLD04_001743"
     CDS             8668..9846
                     /locus_tag="LLD04_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK5648672.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9862..10620
                     /gene="aroD"
                     /locus_tag="LLD04_001744"
     CDS             9862..10620
                     /gene="aroD"
                     /locus_tag="LLD04_001744"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK5648673.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10643..11128
                     /gene="ybaK"
                     /locus_tag="LLD04_001745"
     CDS             10643..11128
                     /gene="ybaK"
                     /locus_tag="LLD04_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK5648674.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11199..12452)
                     /locus_tag="LLD04_001746"
     CDS             complement(11199..12452)
                     /locus_tag="LLD04_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5648675.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12569..13918
                     /gene="gdhA"
                     /locus_tag="LLD04_001747"
     CDS             12569..13918
                     /gene="gdhA"
                     /locus_tag="LLD04_001747"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK5648676.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14030..15376
                     /locus_tag="LLD04_001748"
     CDS             14030..15376
                     /locus_tag="LLD04_001748"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK5648677.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2749 c         3124 g         4513 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     8701 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     8761 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     8821 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     8881 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     8941 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     9001 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     9061 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     9121 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     9181 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     9241 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     9301 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     9361 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     9421 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     9481 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     9541 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     9601 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     9661 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     9721 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     9781 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     9841 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     9901 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     9961 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
    10021 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
    10081 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
    10141 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
    10201 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
    10261 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
    10321 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
    10381 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
    10441 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
    10501 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
    10561 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
    10621 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
    10681 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
    10741 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
    10801 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
    10861 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
    10921 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
    10981 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
    11041 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
    11101 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
    11161 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
    11221 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
    11281 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
    11341 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
    11401 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
    11461 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
    11521 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
    11581 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
    11641 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
    11701 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
    11761 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
    11821 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
    11881 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
    11941 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
    12001 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
    12061 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
    12121 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
    12181 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
    12241 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
    12301 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
    12361 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
    12421 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
    12481 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
    12541 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
    12601 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
    12661 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
    12721 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
    12781 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
    12841 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
    12901 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
    12961 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
    13021 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
    13081 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
    13141 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
    13201 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
    13261 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
    13321 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
    13381 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
    13441 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
    13501 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
    13561 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
    13621 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
    13681 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
    13741 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
    13801 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
    13861 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
    13921 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
    13981 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
    14041 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
    14101 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
    14161 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
    14221 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
    14281 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
    14341 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
    14401 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
    14461 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
    14521 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
    14581 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
    14641 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
    14701 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
    14761 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
    14821 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
    14881 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
    14941 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
    15001 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
    15061 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
    15121 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
    15181 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
    15241 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
    15301 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
    15361 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
    15421 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
    15481 ttcagaataa ggggctgtga caagacgttt gt
//
LOCUS       DAFBYI010000062        15009 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFBYI010000062 DAFBYI010000000
VERSION     DAFBYI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.062"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="LLD04_001749"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="LLD04_001749"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5648678.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="LLD04_001750"
     CDS             complement(1197..2216)
                     /locus_tag="LLD04_001750"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5648679.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="LLD04_001751"
     CDS             complement(2849..3853)
                     /locus_tag="LLD04_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5648680.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="LLD04_001752"
     CDS             complement(3866..4915)
                     /locus_tag="LLD04_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5648681.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="LLD04_001753"
     CDS             complement(4932..5753)
                     /locus_tag="LLD04_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5648682.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="LLD04_001754"
     CDS             complement(5740..6519)
                     /locus_tag="LLD04_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5648683.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="LLD04_001755"
     CDS             complement(6536..7006)
                     /locus_tag="LLD04_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5648684.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="LLD04_001756"
     CDS             complement(7018..7437)
                     /locus_tag="LLD04_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK5648685.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10295)
                     /locus_tag="LLD04_001757"
     CDS             complement(7518..10295)
                     /locus_tag="LLD04_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5648686.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10486..10710
                     /locus_tag="LLD04_001758"
     CDS             10486..10710
                     /locus_tag="LLD04_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648687.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="LLD04_001759"
     CDS             11489..13204
                     /locus_tag="LLD04_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648688.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="LLD04_001760"
     CDS             13207..14970
                     /locus_tag="LLD04_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648689.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4966 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgtttt
//
LOCUS       DAFBYI010000063        14679 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFBYI010000063 DAFBYI010000000
VERSION     DAFBYI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.063"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="LLD04_001761"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="LLD04_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK5648690.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="LLD04_001762"
     CDS             complement(1993..3216)
                     /locus_tag="LLD04_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK5648691.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="LLD04_001763"
     CDS             complement(3247..4086)
                     /locus_tag="LLD04_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5648692.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="LLD04_001764"
     CDS             complement(4103..4783)
                     /locus_tag="LLD04_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648693.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="LLD04_001765"
     CDS             complement(4785..5858)
                     /locus_tag="LLD04_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5648694.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5914..6152)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6195..6428)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6505..7176)
                     /locus_tag="LLD04_001766"
     CDS             complement(6505..7176)
                     /locus_tag="LLD04_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5648695.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="LLD04_001767"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="LLD04_001767"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK5648696.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="LLD04_001768"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="LLD04_001768"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK5648697.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="LLD04_001769"
     CDS             complement(11059..11925)
                     /locus_tag="LLD04_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5648698.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="LLD04_001770"
     CDS             complement(12085..14178)
                     /locus_tag="LLD04_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5648699.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4256 a         3136 c         2504 g         4783 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc
    14581 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg
    14641 tattagatta tgtcaggacg ctaattttac aacctcttg
//
LOCUS       DAFBYI010000064        14193 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFBYI010000064 DAFBYI010000000
VERSION     DAFBYI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.064"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..1127)
                     /locus_tag="LLD04_001771"
     CDS             complement(<1..1127)
                     /locus_tag="LLD04_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5648700.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1210..1545)
                     /locus_tag="LLD04_001772"
     CDS             complement(1210..1545)
                     /locus_tag="LLD04_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBK5648701.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1555..1929)
                     /locus_tag="LLD04_001773"
     CDS             complement(1555..1929)
                     /locus_tag="LLD04_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5648702.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1942..2256)
                     /locus_tag="LLD04_001774"
     CDS             complement(1942..2256)
                     /locus_tag="LLD04_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5648703.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="LLD04_001775"
     CDS             complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="LLD04_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK5648704.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFTEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            6384..6668
                     /locus_tag="LLD04_001776"
     CDS             6384..6668
                     /locus_tag="LLD04_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648705.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            6671..7588
                     /locus_tag="LLD04_001777"
     CDS             6671..7588
                     /locus_tag="LLD04_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5648706.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            7581..8363
                     /locus_tag="LLD04_001778"
     CDS             7581..8363
                     /locus_tag="LLD04_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648707.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            8364..9044
                     /locus_tag="LLD04_001779"
     CDS             8364..9044
                     /locus_tag="LLD04_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648708.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            9037..9933
                     /locus_tag="LLD04_001780"
     CDS             9037..9933
                     /locus_tag="LLD04_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5648709.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            10128..11777
                     /locus_tag="LLD04_001781"
     CDS             10128..11777
                     /locus_tag="LLD04_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5648710.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            12371..13306
                     /locus_tag="LLD04_001782"
     CDS             12371..13306
                     /locus_tag="LLD04_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648711.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            13299..14069
                     /locus_tag="LLD04_001783"
     CDS             13299..14069
                     /locus_tag="LLD04_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648712.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         4183 a         2723 c         2830 g         4457 t
ORIGIN      
        1 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
       61 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
      121 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
      181 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
      241 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
      301 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
      361 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
      421 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
      481 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
      541 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
      601 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
      661 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
      721 taatagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
      781 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
      841 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
      901 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
      961 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
     1021 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
     1081 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
     1141 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
     1201 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
     1261 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
     1321 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
     1381 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
     1441 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
     1501 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
     1561 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
     1621 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
     1681 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
     1741 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
     1801 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
     1861 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
     1921 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
     1981 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
     2041 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
     2101 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
     2161 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
     2221 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
     2281 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
     2341 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
     2401 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
     2461 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
     2521 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
     2581 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
     2641 tttccgtaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
     2701 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
     2761 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
     2821 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
     2881 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
     2941 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
     3001 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
     3061 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
     3121 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
     3181 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
     3241 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
     3301 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
     3361 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
     3421 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
     3481 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
     3541 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
     3601 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
     3661 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
     3721 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
     3781 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
     3841 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
     3901 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
     3961 tcttttcgtc taaagtcaaa gtgaccgttt catcagaacc tttcgttccc ttcgctaact
     4021 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
     4081 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
     4141 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacaa
     4201 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
     4261 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
     4321 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
     4381 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
     4441 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
     4501 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
     4561 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
     4621 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
     4681 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
     4741 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
     4801 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
     4861 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
     4921 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
     4981 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
     5041 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
     5101 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
     5161 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
     5221 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
     5281 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
     5341 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
     5401 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
     5461 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
     5521 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
     5581 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
     5641 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
     5701 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
     5761 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
     5821 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
     5881 gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga ttatcgttcg
     5941 agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt taccttaatc
     6001 ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt ttagctatgt
     6061 taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg ggaaaaagat
     6121 aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa gtggtcagct
     6181 tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat attgtgactt
     6241 gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg tgaaaagtct
     6301 tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg tattattttt
     6361 cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc tggcttcagc
     6421 gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg attaccatgg
     6481 tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag acaactcaac
     6541 cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta cactttcttt
     6601 atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc aataagaaag
     6661 aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta taaccaaaaa
     6721 atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg gaatgatcta
     6781 tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta ttatgggttt
     6841 gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc aggcagcaag
     6901 acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa ctgcaagaga
     6961 taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg aagacctgct
     7021 gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct ctttaggtat
     7081 gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt tgattttgga
     7141 tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa tcatccaacg
     7201 tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag atgaattgtc
     7261 acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag aattatcgaa
     7321 agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg cgacggaaca
     7381 agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat ctagccgtgt
     7441 tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat tggtagaggc
     7501 aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt atttcttaca
     7561 attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata aaagtatgga
     7621 agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt ttttattctt
     7681 ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca acggatacag
     7741 tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg attcttctgg
     7801 ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt acgattcgca
     7861 atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt ctcgttacgc
     7921 ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt gcttacacac
     7981 ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat gttctaaaag
     8041 tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg gctttgacat
     8101 tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc atgggaacgg
     8161 ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg gagtggttta
     8221 cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg caagcaccga
     8281 aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga atgtttattt
     8341 tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt atcgaagacg
     8401 atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa cttcttgatg
     8461 cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat ttgatccttc
     8521 tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc cgacagacat
     8581 cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa gtttccttgt
     8641 tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa ctgttgttac
     8701 gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg tcgcaacgca
     8761 tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg aatggtgtgg
     8821 ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa catccgaaaa
     8881 aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat tttgatagtg
     8941 agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag gcacatgctg
     9001 tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct gttgcttctc
     9061 gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa gagattaact
     9121 aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc agtcgaattt
     9181 cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga tgaaaatgaa
     9241 caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat cgtcggcctt
     9301 tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt actggaacag
     9361 gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc tgttgcaaga
     9421 ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa gcaaaaagaa
     9481 atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt ctctttttat
     9541 gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat aaatacaact
     9601 gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca gaatttattg
     9661 cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt cattcgatta
     9721 acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt cttccagcgt
     9781 ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg gctttacctg
     9841 tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga cgggggcatc
     9901 ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct gaagtgaatg
     9961 acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt tttattttgg
    10021 tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt ttcaaatagc
    10081 gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg ggccaaagag
    10141 aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta gacatttttt
    10201 cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct tatcaaccga
    10261 gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa ttccgttcat
    10321 gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa gtatttgaag
    10381 ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt ttacgtagct
    10441 ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag gaaaatcctc
    10501 aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat cataattttg
    10561 caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt ttatttattt
    10621 ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa gtactaggaa
    10681 tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg aagaaacaaa
    10741 cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca gccgccaatc
    10801 agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact aaaccattaa
    10861 gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa gcagaattga
    10921 tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat ttcgttctca
    10981 ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg ggagaattgg
    11041 aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc cgaccagagt
    11101 ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa gaagcggtag
    11161 ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca tcaggaaaat
    11221 ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc caaacagcga
    11281 ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca atcgaagatg
    11341 atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa cgtttgctgg
    11401 accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta aaaggaacaa
    11461 atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg gagtatccat
    11521 ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag aattattgtg
    11581 agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt gatttcaaat
    11641 taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt ttgaattatc
    11701 ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa cagcgtcagt
    11761 ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa aaagacgatt
    11821 atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac gagtctgctg
    11881 tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg ctgtcttcct
    11941 tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt ccatggcaaa
    12001 gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc attttattta
    12061 tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag cttaacatga
    12121 ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta gtagttgctg
    12181 tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc tatcgaagat
    12241 gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg tgtggaagcc
    12301 aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc tataaggaga
    12361 taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct atggaagtgg
    12421 ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct atgcgctatt
    12481 agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt tagtcaataa
    12541 aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag ttcgagcaaa
    12601 gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa cggtccagca
    12661 aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga tgaaacgcag
    12721 cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg cacgtatgct
    12781 ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg aaccacgttt
    12841 gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg agatgtggga
    12901 gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc attatctaga
    12961 agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac tgatcgagaa
    13021 tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct ttgatctagc
    13081 gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag atgagcagac
    13141 aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc agcttacaag
    13201 acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag aactgttctt
    13261 gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt tattttactg
    13321 cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg gtccaaacac
    13381 tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa atgatcggtg
    13441 ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct ggtttgatta
    13501 tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc tctcaaaaat
    13561 ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg attatttggg
    13621 gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta acgatcattt
    13681 ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt acattattga
    13741 tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct cagtcttatg
    13801 atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta ggtggcgttt
    13861 tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc aatccgatcg
    13921 tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg ccagtagcaa
    13981 catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact tggtatttga
    14041 tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc gttttgacct
    14101 gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt ttgagcgaat
    14161 accgagggtc agctgattga acaccgttta gtg
//
LOCUS       DAFBYI010000065        14125 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFBYI010000065 DAFBYI010000000
VERSION     DAFBYI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.065"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            108..1190
                     /locus_tag="LLD04_001784"
     CDS             108..1190
                     /locus_tag="LLD04_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK5648713.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1190..1993
                     /locus_tag="LLD04_001785"
     CDS             1190..1993
                     /locus_tag="LLD04_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5648714.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2063..2803
                     /locus_tag="LLD04_001786"
     CDS             2063..2803
                     /locus_tag="LLD04_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5648715.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2814..3818
                     /locus_tag="LLD04_001787"
     CDS             2814..3818
                     /locus_tag="LLD04_001787"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK5648716.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3863..5707)
                     /locus_tag="LLD04_001788"
     CDS             complement(3863..5707)
                     /locus_tag="LLD04_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648717.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            5919..6437
                     /locus_tag="LLD04_001789"
     CDS             5919..6437
                     /locus_tag="LLD04_001789"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5648718.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6565..7248
                     /locus_tag="LLD04_001790"
     CDS             6565..7248
                     /locus_tag="LLD04_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK5648719.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7557..7630
                     /locus_tag="LLD04_001791"
     tRNA            7557..7630
                     /locus_tag="LLD04_001791"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7591..7593,aa:Arg,seq:tct)
     gene            7789..8148
                     /locus_tag="LLD04_001792"
     CDS             7789..8148
                     /locus_tag="LLD04_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK5648720.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8271..9902
                     /locus_tag="LLD04_001793"
     CDS             8271..9902
                     /locus_tag="LLD04_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK5648721.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(9964..10605)
                     /locus_tag="LLD04_001794"
     CDS             complement(9964..10605)
                     /locus_tag="LLD04_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5648722.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    10791..10867
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            10887..11195
                     /gene="rplU"
                     /locus_tag="LLD04_001795"
     CDS             10887..11195
                     /gene="rplU"
                     /locus_tag="LLD04_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK5648723.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11222..11566
                     /locus_tag="LLD04_001796"
     CDS             11222..11566
                     /locus_tag="LLD04_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK5648724.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            11579..11872
                     /gene="rpmA"
                     /locus_tag="LLD04_001797"
     CDS             11579..11872
                     /gene="rpmA"
                     /locus_tag="LLD04_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK5648725.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12279..>14125
                     /locus_tag="LLD04_001798"
     CDS             12279..>14125
                     /locus_tag="LLD04_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5648726.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYGFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTA"
BASE COUNT         4557 a         2342 c         2922 g         4304 t
ORIGIN      
        1 ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat
       61 aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag
      121 aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg
      181 taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt
      241 taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac
      301 atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc
      361 aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac
      421 gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc
      481 aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc
      541 aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat
      601 acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga
      661 cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat
      721 tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc
      781 ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac actccaggaa
      841 tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg
      901 catctatgat cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg
      961 atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt
     1021 tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc
     1081 atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag
     1141 tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag
     1201 aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat
     1261 caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga acttttttga
     1321 cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga
     1381 taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg
     1441 gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc
     1501 gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt
     1561 tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa
     1621 tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat
     1681 gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt
     1741 tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg
     1801 ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg
     1861 ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt
     1921 gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc
     1981 ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata
     2041 aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc
     2101 ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat
     2161 cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga
     2221 tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta
     2281 ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac
     2341 aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta
     2401 ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca
     2461 tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt
     2521 tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt
     2581 tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa
     2641 agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc
     2701 tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat
     2761 tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt
     2821 ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc
     2881 aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca
     2941 tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca
     3001 ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga
     3061 actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac
     3121 agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa
     3181 atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc
     3241 ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat
     3301 taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa
     3361 cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc
     3421 aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat
     3481 tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg
     3541 attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg
     3601 gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg
     3661 gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat
     3721 atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg
     3781 cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca
     3841 catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag
     3901 tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta
     3961 cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc
     4021 gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct
     4081 cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt
     4141 ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag
     4201 gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact
     4261 tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt
     4321 cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg
     4381 tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga agtgtattta
     4441 cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca tttttgattt
     4501 ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg attagattgt
     4561 agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg atcgttgttt
     4621 gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct accaagacga
     4681 tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca atgcatgcaa
     4741 gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt aattcaggaa
     4801 gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa agttggaacg
     4861 tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag tagcagatga
     4921 tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg aatggttcaa
     4981 aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc
     5041 aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag tagatcgtat
     5101 agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact tgcggcactg
     5161 attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact ttcggtggca
     5221 gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt ccgatataat
     5281 ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc tgataatctt
     5341 tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg tttcctttga
     5401 ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg aatgtttcgt
     5461 ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat
     5521 cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg tccgcaggag
     5581 aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatgat aatataatga
     5641 ttatgctcca ctttatacgt aaataaaaag attctttttt attatatatg ttattaaaaa
     5701 aagacataaa ggtattcctt tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta
     5761 acgtcaatta tttataataa aaaatgagat aaagatgagg aaatagaatt agaaacatat
     5821 aattattaaa gggaaacatt tttacagcta taatttgaaa aaaatttatc gttattatat
     5881 gattaagaag gattgaataa agaaagaagt tgaaaacgat acctgattac aaaaaaataa
     5941 cttttataag ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg
     6001 ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt
     6061 atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag
     6121 atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg
     6181 ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg
     6241 gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac
     6301 tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt
     6361 gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa
     6421 cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct
     6481 tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta
     6541 taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa
     6601 gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa
     6661 ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt
     6721 ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc
     6781 ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt
     6841 gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca
     6901 gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg
     6961 tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc
     7021 ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg
     7081 atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat
     7141 gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt
     7201 gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg
     7261 gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct
     7321 atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc
     7381 aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt
     7441 aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt
     7501 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
     7561 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
     7621 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
     7681 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
     7741 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
     7801 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
     7861 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
     7921 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
     7981 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
     8041 atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg gattttactc
     8101 aaacaaggtg ggctagctct gttggcattg ctttctattt tcttataaat atagaaaaaa
     8161 taagaattta tctaacagga aactttgtga aaaatattac tttatctcat gaaatatgat
     8221 attctaatga atggtatttt agaaaatttc atgaaaaagg agttagatag gtgttcaaaa
     8281 attatgtttc ctcactaaaa aaagaatttt caggttatga tctttcaaga tttcagaaag
     8341 atcttttggc tgggatcaca gtagctgctg tagcattgcc tttagccttg gcttttgggg
     8401 tatcaagcgg agctgatgcg gccgcaggtt tgattacggc tatcattgcc ggtatcgtta
     8461 ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta
     8521 tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt attgcttgct acttttttag
     8581 cgggtctatt tttacttgca gcaggacttt tccgattagg aacactgact tcttttatcc
     8641 cagcacccgt aattacgggg tttacttcag gtattgctat cattattgca ttaggtcaga
     8701 tcgataattt atttggtgtt cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt
     8761 atcaaacgat aggatttccg ataaacacag cctctttgat catgggaggg ctagtcattt
     8821 tagggatgtt tatctatcca aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta
     8881 tcattttggc aacagcagtt atgctattgt tccatttacc agtagcaact gtgggtaaga
     8941 ttccacaaac attgatcagt agtaatcgtt tgaatatggg agattttagc ttttctgcct
     9001 tgcaacaagt ggcagttcca gctattagca ttgctttatt aggaatgatt gaaagcttgc
     9061 tatgtggtgc ttcggctggc cgaatggcaa ataagccctt agatagcaat caagaattaa
     9121 tggcgcaagg aattggaaat ctgttgcttc ctttctttgg cgggattcct gctactgcgg
     9181 ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca aactagatta gctggtatga
     9241 tacatgcagt tgtcttactg ctctccatgc ttgtttttgc gccagtgatg tcacagattc
     9301 caatgccagc acttgcaggt gttttgattg tgacggcttg gcgtatgaat gaatgggaaa
     9361 cgatcaagga gctatttact aataaatatt ggtcagcagt tctattgttt atccttacga
     9421 tgatttgtac agttatcttt gatttaagta ttgctatcgt gatcggggtg atcggcggct
     9481 gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc attagaaacg atcgattggc
     9541 aaagaatggg gctgccagaa acggaaaagc tgaataactg gacagtagtg tatattagtg
     9601 gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc
     9661 acgatggtgt cattttctcc atgcgaggcg taccaagtat tgatttgacg gcacgtgcag
     9721 tttttgatga atttcaagaa aaagcagagc aaagaggaca aacgattatc tatacttcct
     9781 tgcagccaga agtagaaaaa caactttctc acttgtggga acatacattc acgcatcaac
     9841 atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa atatactgca attagccatt
     9901 aaaacaatga aaaagaacag acaagactgt aaactgaaga ttttatggtc ttgtctgttc
     9961 tttttaatat tgctgtaaaa gtgaagctag tacttgccag tcttgatgga taacaggtac
    10021 aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt ggaagaattc gatagttttt
    10081 ttctgaccat tcgtaggcat ccttttgttc tgttaattgt aagacaggcc ctgtatagag
    10141 ttgaccatga tttttggtaa ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt
    10201 tccaagtgtc gcaattaatt ttggctggac cttcatcagt tcgtaatcca atattggtgc
    10261 atgcgcaaat acttcggaac gagtaggtgt ccgatttgga taaacagttt ctgactgttg
    10321 tgtccgttta ttgatccgat gagtcacgcg atacgggcgg ctccttacag cactggtgat
    10381 atacacctct tctcgtgtta agccagttga agaaagtgcc tgcatcaatt cttttccagc
    10441 ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc cctggggctt ctcccacgag
    10501 catcagttta ggatgaagag gtccttgccc agcgataaat ccttctacag gacggccgtt
    10561 cattcttaaa gaagcttctt gtatcagttc ttttgggtat tccatttatg ctcctccttt
    10621 ttcttttatt ataagaataa aagaaacaca atgcgactat tttatttggt agaactgtga
    10681 ggatagaaaa gcagaagtat tgcttgaatt tttgttttac tttttgattt taaatttagt
    10741 tgactaacgg ttgctgttct ggtaagctag tactgttgta attgtggact ccacagctac
    10801 aaccgctcag aacaagtaat aagcacagca tatgctgtca cttcggatgg cgagtctaag
    10861 tatatcagaa ggaggtgctt tatagcatgt acgcaattat taaaactggt ggtaaacaag
    10921 taaaagttga agtgggacaa gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa
    10981 aagttgtttt tgacgaagtt atcttagtag gcggagaatc tacaaaagta ggcgctccaa
    11041 ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg
    11101 ttactttcaa atacaaacct aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat
    11161 atacaaaagt ggttatcgac gcgatcaacg cttaatagcg tttgagaaag aaggcctata
    11221 tatgatcaaa ggaacattta agcgaaatga cgcaggtcag atcgtttcat tcactttgac
    11281 cgggcatgct gatgctggtc cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt
    11341 agcgatcagc actgtcaacg ggattgcctc attagccggt tttgagccga ttgttgaaat
    11401 gaatgaagaa gaaggcggtt atctttatac cgaagtcact tcaggaatga cacaagaaca
    11461 aaacaatatt gctgagattc tattagaaaa tcttttatta ggattacaat cgatagaagc
    11521 tgaaaactca gaatatatcc aaattaaaac aattaatgat aaataggagg tgcagaccat
    11581 gttgttatct atgaatctac aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa
    11641 cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt gctgatggac aaacagttac
    11701 aggtggatca attctttacc gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg
    11761 tatcggtgga gacgacactt tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa
    11821 aggccgtaac aaaaaacaag tgtctgttta tccagtagct caagaagctt aattgaatct
    11881 tatgattaaa gaggttgtga caagactttt gtcacagcct tttttgttta taaaaataaa
    11941 agttgtagtg gaggctacga agtttacttt gtctttttac acttaatatt tttttgccaa
    12001 tttggaatca ggatgttttg ataactatcg ttttcaattg atggaagcta tattgtttgc
    12061 tatcagtgaa ataattatat ggtaaacagt gttctttttc ttgtttccat tataaaaatc
    12121 gtatcgggat ttttcgttct tttaaattga acatttctat ctatccgcgt gttatcaaca
    12181 aaacaaaatc aggtaaatag ttgacgaaac agaaataaat acatagtatt atgttttgaa
    12241 gcctaagtac aatagtctat agaaaaggag cgataccatt ggcaaaacgc attgaccgaa
    12301 tttatatgta tgtcaaagaa aaaaccgctc atttaacaaa ggcagaatat gatcaaggag
    12361 taacaacaca ggaaattgct gaagtgttag ggattcagcg aacaaatagc agtaaagatc
    12421 tgaatcagct agtgcgcgaa ggaaaactga taaagacaga cggacgacca gtacgttaca
    12481 tatatcaaac actcgttacg cacgtaaagc cagttacaaa acatgtagtc agttacaaag
    12541 aaacgccggt ctctgaagaa aaaaatacac tccaaataga tactaaggat atctttgcaa
    12601 aaatcattgg agctaacggc agcatgaaaa attcagtgga acaggcaaaa gctgcgattt
    12661 tgtatccgcc aagaggatta aattgtttga ttacaggacc taccggatca ggaaagactt
    12721 attttgcaca cgcgatgtat cattttgcaa aagcgcatca tgtaatagat gaggaaacgg
    12781 aactgattgt tttcaattgt gctgattatg ccaataaccc tgagcttttg atgagtcatt
    12841 tatttgggta cgcaaaaggt gcttttacag gcgcagatga agaaaaaatg gggattatcg
    12901 atcaagcgga tggcggtatg ctgtttttgg atgagattca ccggttgccg cctgaaggtc
    12961 aagaaatgat cttttacttt atggatcatg gaacttacag tcgtcttggg gaaacaacga
    13021 aatcccatga agcaaatgta cggatcgtag gtgcgaccac agaagaccca ggttcttctc
    13081 tattagaaac ttttgttcgg cgtatcccaa tcaatatcaa acttccggct tttgaaaaac
    13141 gtcctgcaaa tgagaaagtc gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca
    13201 cgcagcgaaa gatttctttg acagaagatg tagcaaaagc attgatcggc agtgtgacct
    13261 atggaaatat aggacaattg aagtcaaata tccagttagt atgtgcacga ggatttctaa
    13321 atcatatgca atcacccgaa atctcgatca caatcgatga tttgacagaa gggattcgta
    13381 gcggattgat tcaattagca agcaaccgca gtgcaatggc agagctttct aaaatattgg
    13441 agccaaagat aactgtagca ccaaatgata cgatcatgaa aatccaatcg gactcctatg
    13501 agttacctta taatctatat gatatcatcg gagataaagc agctttactg aagtcggatg
    13561 gtttggatca agaagcaatc aatcatttca tttctacaga tatcaacatc catctgaagt
    13621 ctttctataa agaccatggt ttttctttta atgcagacag caaattagca gaatttgtag
    13681 atccgaaaat catagaagtg accaatcaga tttatgtaat ggtcaaaaga tctttaccat
    13741 atgggtttca gcaaaacttt atttatgcta tgagcttgca tatcagttct tttttgaaaa
    13801 aaatcaactt aggagaagaa cggcatacga atgataacat ccgtgagatg gcttttgatt
    13861 ttccagatga atatgctgtt gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag
    13921 tggagattcc agaaagtgaa gactattact tgaccgttct actcgtctcg ttacgggcaa
    13981 atcaagcaag cggcagaatc ggtgtagtcg ttgcagctca cggaaacagc acagcaagca
    14041 gtatggtcca agttgtccaa cagttgttag atgcagagaa cgtacgagca gtagacatgc
    14101 ctttggatat ggatccgaaa acagc
//
LOCUS       DAFBYI010000066        14060 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFBYI010000066 DAFBYI010000000
VERSION     DAFBYI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.066"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            684..878
                     /locus_tag="LLD04_001799"
     CDS             684..878
                     /locus_tag="LLD04_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648727.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1000..1318
                     /locus_tag="LLD04_001800"
                     /pseudo
     CDS             1000..1318
                     /locus_tag="LLD04_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1562..2392)
                     /locus_tag="LLD04_001801"
     CDS             complement(1562..2392)
                     /locus_tag="LLD04_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK5648728.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2600..3934
                     /locus_tag="LLD04_001802"
     CDS             2600..3934
                     /locus_tag="LLD04_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5648729.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3975..4886
                     /locus_tag="LLD04_001803"
     CDS             3975..4886
                     /locus_tag="LLD04_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5648730.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4883..5737
                     /locus_tag="LLD04_001804"
     CDS             4883..5737
                     /locus_tag="LLD04_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5648731.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5734..6351
                     /locus_tag="LLD04_001805"
     CDS             5734..6351
                     /locus_tag="LLD04_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5648732.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6353..6871
                     /locus_tag="LLD04_001806"
     CDS             6353..6871
                     /locus_tag="LLD04_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5648733.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            7169..7627
                     /locus_tag="LLD04_001807"
     CDS             7169..7627
                     /locus_tag="LLD04_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5648734.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            7920..11438
                     /locus_tag="LLD04_001808"
     CDS             7920..11438
                     /locus_tag="LLD04_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5648735.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            11438..12751
                     /locus_tag="LLD04_001809"
     CDS             11438..12751
                     /locus_tag="LLD04_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5648736.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            12744..>14060
                     /locus_tag="LLD04_001810"
     CDS             12744..>14060
                     /locus_tag="LLD04_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5648737.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVY"
BASE COUNT         4516 a         2406 c         3047 g         4091 t
ORIGIN      
        1 aaaattctaa aggattccta accatcttat tagcttttat agggtatatg ttggttgggc
       61 tgctaaaatc atacagtaat gagttgctga atttttcaac atttattaat gatacattag
      121 ttccaagttt gtttttcatt gtattttttg ctgttggtta ttttataata aagatatgat
      181 gactaattta gctcaactat tccagcgtca gctgaagata ttgcaattcc cgattcctta
      241 atcatttatc tggatgagac agaaagtgaa gttgcaacag atgtgcatgt agttgaggat
      301 aaaaatgata gtttaaaaat taatagttca gatactttac taagagtaag tcctatcttt
      361 tcagctgtta tttagatgca agtagcaact aagttaggta gtactcctgc aatggatagg
      421 tatttgtgtg ttcgatatat aagtggtcga agtatcaagg ttatgtatac tggatgggag
      481 atagaagagt taaaaattgg aggtcaccgc cacaaatgga atttagattt agaggtacct
      541 tagatttggt atagttatta aacacaagat ggcctggttt gtagggaaag tcgaagagta
      601 tttttccact tctgcgcccc tttaattttt ctagtaatgc cgtgaatttg tgattaattg
      661 gaaatatgaa gaaaggagag tctatgaaaa caacactagt tctattctat aaaaagcatc
      721 cttattttac acttctgata aatatattgt tggctagtgt tatcgggatt tctgttgaat
      781 atctaataaa taaagatttc ataggttctt gtttttatac ggctttattt cttggacttc
      841 ttgaagcatt ttccatttat aaaaaatcaa aaaaataaaa tagattgccg taagagatct
      901 ttttcatttc atttttttct gaaaacccgt tgtttctgtc agtgaagcag cgggttttta
      961 gatttattgt ctttagacct aattgagcgc gccgagcgat tgaaatacaa aaccacttgc
     1021 tatgcgggtg gagcaataaa gggaaatagt ttagcccgta caaagtagat gtgtaaatac
     1081 cacattgtat tcacaccaaa gtatagaaga aaaataattt ataatcaata tcgagccaat
     1141 attcaggaga tcatcaagag attgtgcaaa tctaaaatat aaatttgggt atcgacactt
     1201 ttgggtggaa ggatactatg tgacaaaagt aggacaaaat gaagcgacca tcaaaaagta
     1261 cattcaagaa caagaaaaac atgatagtgc gttagataag ctgagtgtac gagagtaaga
     1321 cgatcctttt aagggctaag gagtagtaca taaacctttt aagaggtggg caaaactggc
     1381 aaaggcaata tggcttgaac ataaaatatg aaagccagcg tctttaggcg ctggccggtg
     1441 atttgagctt atagctctag agaaaaccac ccttttgaag ggtggttatg attaatgact
     1501 ttagtcagtt ttgctcacaa agcgtgtgga acaaaaaacg ggtgtttcta cgggtagata
     1561 tttatttctt gaatggaggt gtcccataca tttttggtgt tactggttct aattttggtg
     1621 cagcgccttc gatcttaggt tctttcaacc attgatattc gccttcgcca tccagtgctt
     1681 tgccactagc ccaagaacct tcactacttt ggtctccctc tgagaagtta tagaagtcat
     1741 aagcaatgtc ttttcgttct ttgtcacgcg ggaaagtact tgggactaag attgaacctt
     1801 ctttttcttc taattctttc actgcacatt cccatagatt ttgatgatag ctatctcttg
     1861 caaggagaac agacagcata tcttttacgc cacgatcatc gatcatttcg taaatacgtg
     1921 ctacttgaag tcttccttgt gattcggcat ttaaattagc tctaaagtct gctaacaagt
     1981 tgccactcgc aatgatatag gcaccgctcc atggattgcc cacgctgtca gcaggacgag
     2041 gtcctaagcc agctacgatc gcgtgctgtg gattcattcc agagataatg gcaccgattg
     2101 ttggatcttt ttgcatcgct gcttcttgat cttcaacagg cgcaccctct aataaacgag
     2161 caaccattgt cgccaacatt tccacgtggc cgatttcttc tgttgcaatg tccatcagca
     2221 tatctttata tttttcttct cctcggcagc tccagccttg gaacaggtac tgcatagcta
     2281 cactcatctc gccatattgg ccgccgatca gctcctgcag atatttcgcg attaatggat
     2341 tcggacgatc tggttttgct tcaaactgca attctttctg gtgtctaaac atcaaaattc
     2401 ctcctaatct atttttaata cattctcatt ctgctacgaa agattaaaaa attcaaagaa
     2461 agcgctcgcg aattatataa agtattggta tcattaataa tttttttaga atcatacctt
     2521 ataaattgtt attttcagaa caaaatgata tcgttatatt aaaaccggca tcggttacat
     2581 taaaatttgg aggtaaaata tgaaaaaatt attgtttaca gggttgctgg caagttcagc
     2641 attgttttta ttaggaggat gcggatcaag taatgcaagc gatgccagtt caagtgcagc
     2701 aaattcttct gaaaaaacaa gtgatggaag aaaaaatatt gatttctggt ccttttgggg
     2761 atccggtgca cgaaaagaag ctatcgaaga aatcattgag gatttcaatc aatcacaaaa
     2821 cgagatccat gtaaattata agtatcagcc atggggtgac atctggacta aatctttatc
     2881 tgcaataaca gctggaaatg cacccgatgt tattgtacag gacataaatt ccgttgccca
     2941 aagagcagaa gcacagcaag caactaatct ctccaaatat gtagaaaaag acacttctaa
     3001 ggatttttat ccgcagcttt gggatacagt agaatatgaa ggtgatcctt atgcgatccc
     3061 attcaataca gatacacaag tcatttttta taataaaaag ttatttaaag aagctggaat
     3121 cagtgaacaa gatctaccga caacttggga tgaattagaa gaaacagcac gtaaactaga
     3181 taaaaaagaa ggaaaagaat ttacacgcat cggtttttat ccattatgga atttaggggc
     3241 tgatgtatgg gcgctaaacg cagataacgg gactagttgg tttgacaaag atgaaaaagt
     3301 caagattgat actaaaaata aagtggaagc cttggagtgg atccaagact ggcaagatta
     3361 ttatgggaaa aatacgatca ataaattaga agccgaattt gggtctggtg tatctgatcc
     3421 atttatttcc ggattagtag ccatgcgtgc ccaaaatatc aattattact ctagtttgat
     3481 ggaaaatgca ccggatgatt ttgattttgg cgtgatgcag ctcccagaaa aagaagaagg
     3541 ttcaggccat tggtcgtggg gtggcggatt cgtcttggaa gtaccatacg gagcaaaaga
     3601 tcctgaggcc tcttatgaat tcatcaaata tctttcttct ccagaagtac aagaaaaatt
     3661 cggcgaaaaa agttttgata tcatggcgag caagacagcc aatgagaatt tagtgaataa
     3721 tgacaaccta acggataaag gaaaaatgat ctatcaaatg gcggacgaaa actttaaaaa
     3781 tacggtgatc acaccagttc ctttagatgc tccggattac acgacattag tgaatgaaca
     3841 gattgaccag attcttttag gaacaaaatc accaaaagaa ggattggcag atgcacaaaa
     3901 agcagtagaa aacttagtca agcaaaatca ataatccatt tttgactgtc taggtacaga
     3961 ggaggcaaga aaatttgaag cgtttatcta tccgaaagaa aaaagaagca agatgggcct
     4021 atttatttat ctcgcctttt attatcgggt tcttggtatt tatgctaggg cctatgcttt
     4081 tttcagttat tggtagtttc accgattata atttgacatc caagatgaat ttcattggtc
     4141 tagcaaattt caaaagaatg ttcgttcacg atgatttatt ttggaaatcg ttatacaaca
     4201 ccgtttattt cgtcatcttc aatgtaccgc tgacgacgat tttcagtgtg tttttagcca
     4261 cagtactcaa tcaaaaaatc aaaggaatca gtgtattccg tacagctttc tatttaccgg
     4321 cagtcttatc aggagtagca gtctacatct tatggatgca gctgctttct ccatctgcag
     4381 gattgatcaa cacatttctg ggctggttcc atatccctgg accagcttgg ctgtttgatc
     4441 cacaatggac gaaacctgcg ttgattttga tgaaaatatg gagttctgga ggagcaatgc
     4501 tactttactt agctacatta caaaatgttc ctagatcttt atatgaatca gcagaaatcg
     4561 atggagcagg cgtttgggga aagttcaaaa gtattacttt gccattaatc actcctatta
     4621 ttttttacga tgtgatcacc tctacgatcg gttctttcca aatttttcaa gaagcttatg
     4681 tcatgacgaa aaatggaaca ggcggacctg caaattcttt gcttttttat aatctgcata
     4741 tgtggaacaa ggcgttcgta gcttttgata tgggttatgc gatggctatg tcaatgatcc
     4801 tgtttctgat cgtgttggtt ctgacgtttg tcaatttgaa gcttgcacct aagtgggtct
     4861 attattctgg aggtgacggc aaatgatgga ttatttcaaa aagaccagtg tgaatcggca
     4921 aacaaaaaaa atactaagtt atgtcctcat gacagtaata ggaatcattt tgatcattct
     4981 attgctttgg atggtgttta cttcattgaa accaatggaa gaaatcgttc gttatcctcc
     5041 gacctttttc ccagaaaaaa tcgtatggga aaactatctg gatacgattg ccgcctttcc
     5101 tttttggcgc tatgcaagaa atacattatt catcactgtg ctagtagtga tcggtaatgt
     5161 cctttcaaat tcttttattg cttatggatt tgcgaagctt gattttcctg ggaaaaagct
     5221 gatgtttgct ttggttcttt ctacgatgat gatcccgggg tttgtaacga tgatccctca
     5281 atatgtatta ttctccaaga tcggctgggt aggtacttat ttgcccctga tcgttccatc
     5341 gttcttcggc aatgcgttca atatatttct gatgcgtcaa ttttacttat cgatcaataa
     5401 tgaattgatc gaagctgctg aaatcgacgg agcgaatcat ttgtatattt ggagtcattt
     5461 gatgcttcca ttgacgaaac ctgccttgat taccattgcg atcaattcct tcaacgcagc
     5521 ttggaatgat ttcttaggtc ctctgttata tatccaagat caagaaaaat acacgttaca
     5581 gattggcttg caagtttttc agaaccaagc aacgacacaa tggaattatc tgatggcagg
     5641 agcaacactt gtcttgatcc cgacgatttt gctgttcttt tttgcacaac gttatttcat
     5701 tgaagggatg gatttgacag gaggttcaaa aggatgatga atcgatcgat tggtaaaaaa
     5761 gccaatgatt atttcttaga aatcattgaa tggattccac gtcttatcca tttacagcta
     5821 ctatggttcc tgtgcgtctt gcctgttttc acattaggaa ctgcaagtcg aacagttctg
     5881 tatatgattt atcattacaa aaaaaatgaa gagaaaaagc tcggttcact attctggaaa
     5941 aagtttcgtg agaacttctc tctttatgga aagcaagata gtttagcaag tgtttatttt
     6001 cttcttctat tgatcgacta tcagattttc cgctattggg gtgggggaat tggctataca
     6061 ttgatgtata ctacgttgtt tttgcttcta ttaagcgcgc ttctctttag ttacaaaatc
     6121 ttgcttcaac tggaacaaaa agaagcttct tggattactg catttttctt gttttttaat
     6181 gatttcaaaa gcgcgctttt ctatttggca ggcacgttga tccttcttgt cgcattctgg
     6241 tttgcaggac caatctattt tgctttatta ggtttcagtt tcttgtttta ttttcaagtc
     6301 ctgcttttta tccatcgtac gcaagaaaag gttcttaaaa aggaggaata acatgttgcg
     6361 tttgattgct aaaatcgatg atagtattat caacaagttc gccaagcaac ggaaaacgga
     6421 tagtcgaaaa atcaattttt tgaaatttat cagtaatatc tctaatggtg gttttatttg
     6481 gttgctcctt gctgctgttt tagtcttgat acctgatact agagaatatg gtctagccat
     6541 tcttatcggc agtttgatct atttttttgt tggtttgctt tttatcaaaa aaacgacaag
     6601 acggaaaaga ccttatgaaa aaaatcgtcg gcttgtcctt tacacaagta aaccgaagac
     6661 ttcttctttt ccttcaggac atacagcgct agccttttta tcagtagctg cgttgttcga
     6721 attaagtcca gttgcaggaa tattagcctt tttatttgct tgtttgatcg cttattcgag
     6781 gattttttta cttttacatt atcctactga cattttagga ggagctattt taggaagtgt
     6841 tattggcatc ctatgcgctg ccgttatctg aattaattgt acgaataaag catgagtgcg
     6901 taaatgcgca tttgtgcttt atttttttgt ttcgaggaaa gcgtagttgt ttattcattt
     6961 gtcttaataa cttaagtttt gttaaatgag aacgttataa aattttataa tttaatttta
     7021 ataataaatg ttgtataatt ggtgtttttt aactaaaaag gatatcattt gatttaaaat
     7081 taaaattaaa ttaaattata attagatggg cattgttttg ttgattgttg tgaaagaaga
     7141 caaatatttt tgaaaggagt ataaggatgt gaaagggtta aaattgaagc ttttgcagtt
     7201 agtaactgtc agtttgctat ttggaccata tttccagtca gtctatcata tgacgaccct
     7261 ggatgtagct gctgagtcaa cagaaacaaa ggaggatgag gtattcttag atgaggacta
     7321 tgcaaaaatt gaagggaagt atattgaaaa agaagaaacc cttgaatggc atctggcttt
     7381 tgaaaaaaag gaaagtgcca atgaaggtag aatacgttta gcaatcgatg cggcagccgc
     7441 cgggattggt accgtaaaaa acgttcgtgg aacaggtcta tcctcctatg atgaagatgg
     7501 agaagaccta aaagtcgaga cgatcgatgg tcaagaatgg tatgtcggaa aagtttattc
     7561 aaaagaagca gaaaccggca cattgatttt tgaaacagaa aagatccccg atgtgaacga
     7621 agggtaacta cctcttcaag tggtggttga tgaaagaata gatgaagttt acaacgaatg
     7681 actatacaaa agtgttagga aattggacac aaagcaatga acgctatcaa ggagacaaag
     7741 gctcgccata tgacggagag aacttgatcg atgatagtga ttatacaaac gggggagcga
     7801 actggcgaaa ttacgactat gctactaata atggatcaac tgcaggcgaa gaagatagaa
     7861 acgttccagc tcaaaccgtt cagctttggg gaaatgaacg gaattttgaa aacagttact
     7921 tggattataa cggggcgtac atcaaaaaat gggtagaacc tgttctgcca agcagcacag
     7981 catctgatct acatcctgaa gatgcaacaa ccctttacaa tgtctatcta gatgtcatcg
     8041 ggggcgagaa aaaggaaatc agtccaatcg atatcgtttt tgttctagat aagtcagcga
     8101 gtatgagtga attaaccgct gggaccaata gtcaaactaa aaatgctgcg ttgattgagg
     8161 cagtaaacga aatgagtaaa gatctgctct ctgacccgag tctggatatt cgaatcggta
     8221 tggtgaattt ttaccataat agtacagcta tcaataacca tgagcaaatc agttcggaca
     8281 tctttccatt gacaaatgat atcaatcgtt tgacgggaag tgagaataca gcattaaatc
     8341 gtacaccaat cggaggaact ccgcttacac tagggcttaa aaatggctat gaaacattgt
     8401 ataaagacaa tggcggagaa aatagaaatc cagaaaaaat cctaatcgtt gtgggagatg
     8461 ggacaccgac cttcagttat gcaccgattc agtcaagtta tcgtacgagc actaatggag
     8521 cttggagcaa ctggactgtg atggaagata agattgctga agataatgac gttttgttca
     8581 gaaattttga ggagttttct gggaatactt ctaatgcagg gtttactcat ccagttacct
     8641 atgcatctga ctttaaccgt ccagaagatg aagtaaatgt acactatcgt tatggagagg
     8701 taaaagaagg agataataaa gcgactcact gggttggtga tggttcttct aataacaata
     8761 cgaatggcag tcctacttcg caagaaaagt catctgcgat caacacagtt gcttatcatc
     8821 attggctgaa aaacaagtat caagagaacc cgccaagtat cttctccatt ggtttaggaa
     8881 tcgatggaaa cgttagcggg agacaacgac tagatgctat tggacgaaat gtattgaaaa
     8941 atattgctga tttagaagaa gatggcgtaa caccgcgata ttacaatgcg aacaacaaga
     9001 atgacattgt tacagccttg gaggatatct cttctacctt caagagaacg atccagcaag
     9061 ccacacttta tgatgaaact ggttttaatg tctcaccatt tggagctggc agatcggcag
     9121 atattcagtt ttaccatcta gatccaggag aaaacggcat gaagtaccaa gagccagaaa
     9181 tatgggatga agaacgtcat ggacaagccc caccagaagt agtggcgact ccgacaacgt
     9241 atgtcggacg ggaaaaccaa gcttataaat tttctccgat ttctttaggt gaaggggaaa
     9301 tggtcaggat caagtaccaa gtaaaacttg atgcgggagc tcaagacgga aatttttgca
     9361 cagtgaacaa tatggcctat attcagaacc tggaggatta tgagaatgat atcactactg
     9421 gacggatgta tttcccatct tcttccattc gttacgaaca taaaggccgt aatttccaag
     9481 tcagcaagaa gggtcaaaat ggcgagccgt tggctggagt cgaatttgca ttatatgacc
     9541 aaaatccaga gagtggggaa gccgaaccaa tcgaaacaag taaaactacc acggacggaa
     9601 tagcgatctt tgaaacgaag atgccgattt taggagatga aactggagca atcagcgaca
     9661 tattttggat cagagagatc gctggaccac cacgttatca gttagtagat gagacgtatc
     9721 aattccaaat caaaaaatat gcagccaacg aagatgagat gttaggtcac tacgagcttg
     9781 tagatgaata tacatctggt tcgagagtcg gcggaccgga aggtaaagat ccgaattcct
     9841 attatgaagg agaatatcac aagctttctg ttgtacaaga gcctaacata gaaggcacag
     9901 agtatgatga gttgtatgtc aaactggaaa tgcgaaatga attcaagcca atccacatga
     9961 atatcaataa actaattgaa ggcagtaata tgccaatcaa tggcgcagag tttgaacttt
    10021 atgaaacaga tggtaacgga ctggaaatcg gatatccagt agcgaaaggg ctttctggtg
    10081 tgaacgaagt agaggaagag aaagggagac tcacgttcta ttcagtagat gaagacggga
    10141 aatatgtaat cgtggatggt gagaaagtta ttcatccaat cggagagcca gattcatttg
    10201 tttataagga tagtgaagaa actaccaact atgcagaata caaaatcatt gagagccaga
    10261 acccagaagg tttcaaagaa cctgatgaaa atgacacatg gatcttgcga ctttacgata
    10321 atgctatagg gactatccaa ctcaagcaaa caggtgaaga aaattggcaa tatctcacgt
    10381 atacaacaga tgaagaggat cgtttatggg tagcattcac tgcttacaat acgtttgatt
    10441 atcgggagat ccgagttaga aaattagacc atgaaggcca tacattagat ggtgcaggtt
    10501 tcgatatcag gaaagtgggg gattcttcct tcccgctgta tcgagcatac actggtaatc
    10561 cgttagaacc agaagaatcg aaacgtttgc ctgggatcgg atacttctat tctcgtgccg
    10621 taggagtaga aaagtatgtc gatttaaatt acgaccaacc tctgcatctg tctattggtg
    10681 aatatgaggt ctcggaatct gtcgcaccaa atgggtatcg gctatctgat caagtgttcc
    10741 agtttgcact gaatgaagaa ggtaagttta tcattgaaga tacggtcatc gaagacgaaa
    10801 ctgctccctt gccggaaggc tatgaaatca tcgatggtgt actacaagta accttagaag
    10861 atgaactagc gccaatcgat ttggaacttt tgaaaattga cagtatgaat agccgacgtc
    10921 ttgaaggcgc agaattttca attgagaaac aaaatagtgc aggagagtat gaaatcatcg
    10981 agggaggctt ggcaccagat ccaaacgatt cttccctatt caatgcttca gacttgttgg
    11041 aaggggtcta tcggatcaag gaagtgaagt cacctgacag ctatagaaaa cttccaggtt
    11101 actttatttt agaaattagt tatcgagaag aacctgacat agaaaatgat cgggtagttc
    11161 caggaaaaga agcgggtaca ctaaaagttg agatttcgta ttatccaaac gaagaagcag
    11221 agacgcctga tctacagcaa gagttagaat atgaactttt ggagacaaat cgaatcagat
    11281 tacagcttag tgtcgggaac gatcctgaaa atccgttgcc tgctacagga ggatccggac
    11341 gattattcta tatcctgctt gctgcattct tcatggcgct tgcaggagca gcctatggtt
    11401 tgtttgtcag acaatctaaa aaggagggag caaggtaatg aaaaagctgt tgtgtttgat
    11461 gcttcaatta cttgttttac taccgcttgc agcagtcctt cttccacatg cgatgtcggc
    11521 agaagaatca ggggaagatg tcgtggaatt cgtgttgcat aaacggatga ttcgagatat
    11581 cgattataac gatcaatttg aataccatga aaatgatggc ttggctatct ctgaggaagc
    11641 aggggaaaca gcagatatca tttcacagac ggtgccacta aatggagcga catttgccat
    11701 ttacgacatg accgattatt atcatgaaaa aaaagctgct gaaaacatga ctcctaaaga
    11761 attcgtccag caaatcgctg aaacgaacag aaacaatatc cgtcagctaa tcacagaaga
    11821 aaggcttgtc caaataggga cttcggtaac aactgggaaa gatacggttg gcggattagg
    11881 agacgggatt gcacgtatca aagcacctaa gaaaaaccaa gatcgggatg cagtttattt
    11941 gattttcgaa gaatcgattg atccagaagt tggattggat gttgatatcg atcagacagc
    12001 tatcccaatt gctgctattt tgccaattta tcacccgaca gaatcaaccg aggaactgca
    12061 agagattcat atctatccta aaaatgtcgg ttatctaaga gatccatact tcttcaagta
    12121 tggacgtcaa agaggaacaa ctgaaaaagg aaaaccatta gaaggtgctg tttttgctct
    12181 atatcagatg atagaagggg ggaaatatta tttagatatg gctcctgcta atgacttgaa
    12241 aaatcaatgg atcaaaccag cagacaatga tcctttgaat gacaaaaatg taagcaaatt
    12301 catttcggat aaagagggcc ttgttacgac aggacaacgt ttcttgcctt ctggcaccta
    12361 ttattttgaa gaattaaaag gtgtggaagg ttacgagatg gatgaagcaa gtaagagaat
    12421 cgaggtcatc atccctgaat tttgggtgga tgcaaaaggc caaccacaat atgtagtagt
    12481 taacggacag gagatggctg aattagaatc cgggaaagtt ccagtctctg cctatgaatc
    12541 cgcaacacca cgtgtttata acgagaccgt aaaagaaaca acggacaaac cgaaagagcc
    12601 gagtggtcca agcaatccag gtcaatcagg aaaatggtcc gtattcccgc aaacgaatga
    12661 agcaagaacg ttgatttctt tactaggaat tcttttattg ttaggtgctg ttttattaat
    12721 aaaaaaagaa cggggagaaa aaaatgaata aaaaaatgat tgtcacggga atgagtgtac
    12781 tgttactcgt tactgcggga atagggattt tcgggaacgg aaaactagtc aaagccgaag
    12841 aagtcgcaca aaaaccaagt gaagtgacga ttacattaca caaaaaaggt ttctcatctg
    12901 ttccggaaga acgaccgaat agcggattgg tttctacgga ttttggtgaa gaaaatattc
    12961 caggagtcga ttttgatttg tttgacgtaa cggaagtata ttacgatttg attcgagata
    13021 atcctttaac accggaacga gaagacggtc ttaattctgc cgaagcgatt gaatggattc
    13081 aaaaacggca cacagaaagt tggtttttaa aatatcgact gacaagtatt gacaaacaaa
    13141 caacgaatga agctggagaa gcggtattct ctaccgtcca agtgacggaa gaagcacctt
    13201 cttctagaga taaagtctat cttttcttag aaacttattc gccagctcat atttccagaa
    13261 tcgcgtctcc aatggttgtt atgatgccag tgatgatgcc tgatatggtg gatggcgtgt
    13321 gggatggttc tacatggaaa gacacataca atacagatgt gcatttgtat ccgaaaaacg
    13381 aaatcagaga agcagataaa cagatgaatg tagaggaaag cgaccttaga caagtaacga
    13441 ttattaatga agctggagaa caagaaacga tttcttatat tgatctagaa agaggaaaaa
    13501 cggcttctta cacgatcaca gcacctattc cttatttcat cgattctgtc cttgaaaacg
    13561 gaagcgctgt tatcaaaaat tacaaaatca cagatacgcc tacagttggt ctaacttatt
    13621 atgatcagga aatagaagtg cgagccggcg agacgatttt aacaaaagga caagattata
    13681 tagtagaagt agtaagcaac gggtttgtcg tgacgatttt gacagaagaa aatggagtag
    13741 ccaaagtaga tactttaggt agattagcag atgcacgtgg aggagattta acgatcacgt
    13801 ataatttgaa ggtttccaca gaactagaag cggatgattt ccataacaac acagcggtta
    13861 ttgaaatcgg acgaaatgat gaatttgatt atgaagaagg agtagagccg ccagagaaag
    13921 tcacaacagg tggaagaaag tttgaaaaat atgatgcatc aagcagtgaa ttgcttaaag
    13981 atgcaagatt cgaattgtgg aatgaagacc gttctgaata cgcaatattt tataaagggg
    14041 agtcgccttt agctgtttat
//
LOCUS       DAFBYI010000067        14013 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFBYI010000067 DAFBYI010000000
VERSION     DAFBYI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.067"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(609..1544)
                     /gene="whiA"
                     /locus_tag="LLD04_001811"
     CDS             complement(609..1544)
                     /gene="whiA"
                     /locus_tag="LLD04_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK5648738.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1578..2576)
                     /locus_tag="LLD04_001812"
     CDS             complement(1578..2576)
                     /locus_tag="LLD04_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK5648739.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2573..3457)
                     /gene="rapZ"
                     /locus_tag="LLD04_001813"
     CDS             complement(2573..3457)
                     /gene="rapZ"
                     /locus_tag="LLD04_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK5648740.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3592..4323)
                     /locus_tag="LLD04_001814"
     CDS             complement(3592..4323)
                     /locus_tag="LLD04_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK5648741.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4327..5592)
                     /locus_tag="LLD04_001815"
     CDS             complement(4327..5592)
                     /locus_tag="LLD04_001815"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK5648742.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5855..8671)
                     /gene="uvrA"
                     /locus_tag="LLD04_001816"
     CDS             complement(5855..8671)
                     /gene="uvrA"
                     /locus_tag="LLD04_001816"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK5648743.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLTDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8681..10675)
                     /gene="uvrB"
                     /locus_tag="LLD04_001817"
     CDS             complement(8681..10675)
                     /gene="uvrB"
                     /locus_tag="LLD04_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK5648744.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10934..12436
                     /locus_tag="LLD04_001818"
     CDS             10934..12436
                     /locus_tag="LLD04_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK5648745.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12438..13175
                     /locus_tag="LLD04_001819"
     CDS             12438..13175
                     /locus_tag="LLD04_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5648746.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13220..13393)
                     /locus_tag="LLD04_001820"
     CDS             complement(13220..13393)
                     /locus_tag="LLD04_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648747.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            13369..>14013
                     /locus_tag="LLD04_001821"
     CDS             13369..>14013
                     /locus_tag="LLD04_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648748.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4042 a         3024 c         2427 g         4520 t
ORIGIN      
        1 tactgcaatc ataatgatag taaaagttgt aaaaaagcac attttttgcc ctccctttat
       61 aaacatcgat tctcaaattc taaaccaaat cttgatagtt caagttcagg tataacgtat
      121 accaaccata catcccaatt actatttatg aacttatgat tatcactgaa taaatcactc
      181 catccccttg ttaaaacttc tacattaatt acacctggtt gttttcgact agctaatcca
      241 atgaacttat caaccatcct tatccaccaa ccacgagaat cttgaccaga agtaagaaaa
      301 acagttctca aggttgatcg agcagttcct tccagttttt aattaatctc cagttataga
      361 taaatacttg atcctatcta tatcaggact tctatattaa gcaaaagttt atgtataaaa
      421 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aaacaataga
      481 agcctttcaa cattatgtta ctccagtaaa gttggaatta tagtttgacg gaagaaaagc
      541 aagtatagct tttaaaaaaa actgatacat tcgcatctcc tgcttcatgt atcagttttt
      601 tctttgaatt aaactgcttc ttcacgcagt ttttccgcaa attcatttat tttgcgaatt
      661 cggtgattaa ttcccgattt tgatattgca cctgagggaa tcatttcccc taattctttc
      721 aagctgacct ctggatgttg aagccgaagt tctgcgattt cctgaagttt ttcaggaagg
      781 gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt cgatttgttt agaagccgca
      841 tcaatcgtct tgttcagatt tgctgtctca caattgacta gacgattgac cgaatttctc
      901 atatcccgta cgattcgaac atcttcaaat cggagcattg agttggttgc accaatcaat
      961 gttaagaaat cagcaatttt ttctgctcct tttaaatacg aaatatagcc attgcgccgt
     1021 tcaagcgttc gagcatgtag accgtaataa tttaacatat cgcaaatgtc ttggttatgc
     1081 tgttcataaa tagaaaatat ttccaaatga taacgactcg tttccggatt attgactgaa
     1141 cctgaggcca taaaagcacc acgtaagtaa gaacgcattt tttgcgaatt tcccataatc
     1201 tcgtccgaaa cgtgtccatt gaacatcata ccatccatga tatcaagatc catcaacaca
     1261 tcttttgtgc cttgcttcaa ccggacgata tagacattgt tcttttttaa tttcattttt
     1321 ttacgaacga gtagctcact tcgtacctga taatgatctt tcaaaagaga atagatacgt
     1381 ctggcaattg cagcattttc tgtttggacg ttcaaaataa actgctggtt cacaatacct
     1441 aaagagccgt tcattcgcag caaagcagct aattccgctt tcgcatgttc tcgatgaact
     1501 tccaaaccag ttagttcttt cttcacatct gcagcaaatg acatcctatt ctcctccttt
     1561 ctccaacaat aaattgctta atattttgaa ccgaaaacta aacggaacaa ttcttctact
     1621 actttgtctc catcgtggaa gacaccacca tctttcaatt ctaaaaagtc ggtggaaatc
     1681 actcgacatc cttcatttct caatccgttg aagtcgtgtg cgacttgtac tagatactca
     1741 tcatagatct ctgggtccat ataatcttca ggcacttttt ccgtattgac caatactgta
     1801 tcgataaaag gctgattcaa atgcttgtgt aaaacacgta tatgatcggc atctgtgaaa
     1861 tgttccgttt cccctttttg ggtcatgata ttacagatat agacggtttc agcagttgtc
     1921 ttgagcattg cttcgccgat ttctggtatc actaaatttg gaaggatact ggtgaataaa
     1981 cttccgggtc ccaaaacgac catgtcggcg tccataattg ctgaaacgac ttttcgagcc
     2041 gctttggctt cacttttatc atttgtgttg cagacataca cacggtcaat ggttttacgg
     2101 ttgatcgcga ttttagattc tcctactgca gtcgttccgt ctttaaaaac agcatgcaag
     2161 gtcaaaggag tttctgaaga aggataaaca tgtccatcta catgcatcat ttttgctaat
     2221 aattgaatcg cctcataagt gcttccccgc atctcggaaa ctgcggcaat gatcagatta
     2281 ccgatcgtat gattggctaa aaactgatct tcctttttga aacggtattg gaaaatatct
     2341 tcatagaatt gtggcatatc cgataacgcc actaagacat ttctcaagtc accaggagga
     2401 gccatagcga tcgattctct gattgcaccg ctgcttccac catcatctgc aacagtcact
     2461 actgctgtga tatctgctcc ttgatttcgt aaacttttca ggataactgg cagaccagtg
     2521 ccaccgccga ccactacgat ctttggtttt cttatccggt aggttttcat tttcacgacc
     2581 ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt cacattgact ttatagtttt
     2641 tccctaaagc atttgccaaa cgttcagtca atgccacaga acggtgttga ccgcctgtac
     2701 agccgattgc aactgtcaag ctgctttttc cttctttaat gtatccaggc aatacagttt
     2761 ccagaagatc caaaaattta tggtagaatg attgcgtttc atcaaaggac atcacataat
     2821 catagactgg ctgatctttt ccggtcaatg gtcttagttc cgggatataa tgagggttgg
     2881 gcaaaaaacg cacatccatc acaatatccg aatcgatcgg caacccgtat ttaaaaccaa
     2941 atgatacaaa ttcgatccga aatccttggt ctccctttga tttgaatgac tcattgattt
     3001 tttcacgtaa ttgtcgtggt gtcaatgttg tcgtatcaat gatcacggaa gcttgggctt
     3061 tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc agtgatcaaa ccgtccatcg
     3121 ccattggatg tgctcgtctt gtttctttgt agcgagccac tagttcttcg tctgaagcat
     3181 ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc gatttcgatc agcatgtttt
     3241 ggatttcttc aaagaaggaa cgagaacgca aatccactcc taaagccatt tttgtgactt
     3301 tacctgattc tttgatcaat tcccagaatt ttggaatcaa tcgaggcggc atattatcga
     3361 tacaaaaata gcccatatct tcaaaacttt ggatagctac tgttttccct gcaccactca
     3421 tacctgttat gatgactaat tgcaaattat ctgtcatatt tacgaacccc tctcacattg
     3481 tgcataataa aattatatca tcccgggcgt atcattacca gttttctttt tcttttacaa
     3541 aaagatttgg accaaatatc ttttaggata tttgccccaa atccaatttt tttacttttc
     3601 cgatgctgta tccaatgcct cgcttctttc agctaatgca cgttcgatcg ttcgatccgc
     3661 taaaatagat tgagcatcga tcactgggta atgattatct tcagcaaatt catgcatcaa
     3721 agataattct gtgcaaccta ggatgacttt ttcacagttt aggcgctcta ccgcttcctc
     3781 taagatttcg taatagagct cttgattcaa ataatccgat tccttgatct catgatagat
     3841 caggtaattg attttttctt gaagcgcagt atcaggaatc actgtttcga aaccaagatt
     3901 tttgacttcg cgttcataga ttccagcttt catacttcct tctgttccta gaatcgctac
     3961 tctccctgtc gtatgctgac ggacaagttc atttgctgct tctcttggca tatgcaagat
     4021 aggaatatcc gttgccgctt gtaactcttc aaaaaagtag tgtgcggtgt tacaagtcaa
     4081 cacaatgaag tttggtcgca ataaattttg tttctcaata tcatccagca gaaacggcat
     4141 tggattttct tctgatcgat ctaaaatata agctgtacga tcaggaactg ttgcatgatt
     4201 gaataagaca taattcaaat attcttgatc ttttgtagct ttcgttcggt ggttgatcaa
     4261 gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct aaaatactga aaaaattctc
     4321 catgaactag ccttctttca catgaaaata ctttttgaat cttggaatat agttatggaa
     4381 catatatttc atcaacaacc agcgcttgat tgatcggtct ttttcataaa atacagtagt
     4441 accgtaacga ttttctttga tcatttgctc agctagtttt ttgtcttcat tttcccgagc
     4501 atattctaaa aagatttttt tgggcacacc catccataat cttgctttat ctgattgatt
     4561 cgttccatag gtcgtttcaa caaagggttt gttgaacact cgatcttctg tgacaaaacg
     4621 agccagattc aagccgttca atgtcacaaa gaaactgcta cgaccttgtc tcaaattgat
     4681 ttcaaataat ttatattctc catctctagg atcatacttc atatcgaaat tggcaaagcc
     4741 tgtatattcg attttttcca aaaatgcctt aatggtttga tagatttttt cgttataatc
     4801 tggcatgatc acaacgtaat taccaatcga tgctggtgta ggatcttcta atagtggatg
     4861 accaaggcac atcatccgaa cttgatgatc ttcatctaca tacgcattta gcacacgcat
     4921 attactatca tcacccggga taaaatcttg tacaatcatt tcacttttat aaccggcttc
     4981 gtatatccgg cctaaaatta aatcgaactc ttctctagtc tctaaaataa aagctttttt
     5041 tcttccttca aattggacag aaagatactc cacactgttt gcaggtttca aagcaactgg
     5101 gaattcaaaa ggcaattcct gttccaaatg tccatttact aacatttctt ctctgacgat
     5161 caatgtttta ggatacggaa gatcatattc ttcacaaact tcataaaagc ttactttatt
     5221 gatcagcctt tcaaataaag aataatcaat atatggacag ataaaatatt ctgacagctc
     5281 ttgtttatgt tgggagatca attctgcata tccatcacca caagcaatca aaagatattt
     5341 tttactctta tcagaatatc tttcttttgc caaacgaagc atcgtctcga taaatacagg
     5401 gtctttatca aatcctggaa taacttctac attgacaatt ttactgtagc gggtaggggc
     5461 taactgatcc gaagcatatg cttggcagat tttgccgtat gcttcattaa aagaacgtgc
     5521 cataccataa acgttcatgt cgcttcctaa taatataggg ataaattctt cattttcaat
     5581 actgttcatc attatctctc acttactcat aatctcttgt ttttcttttc caactacaca
     5641 aggaatatca taccataaag aaaaaagcaa tcaaaggaaa gtcctccgat tgctcaattc
     5701 ttttttgaac ggattgaact tataggaagt ttttatctat accaatcagt gtagaaagaa
     5761 ctaccatgta atgattctag aacactgata ttaataaaac aagcaaaaat tcattttttt
     5821 atgttttttt aactatctca gctatctatc ctctttatac cattattttt ttcaaatagt
     5881 gacctgtata actttctttt acgttaatga tttcttctgg tgttccagtc gcaaggatcg
     5941 ttcctccacc ttctccacct tctggtccta aatcaatgat ataatctgct gttttgatta
     6001 catctagatt gtgttcaatc actaaaactg tgttaccagc atctactaat ctttgtaata
     6061 catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt cgttggttca tctagtatat
     6121 agaaattctt tccattagag attttgtgca attcactggc aagtttcatc cgttgtgcct
     6181 caccaccgga caatgtcgtt gctggttgcc ccatcgtcac ataacctaag ccaacatcaa
     6241 caatcgtttg cagtttgcga tgaatctttg gaatgtgctt gaagaattct acagcatcct
     6301 ctactgtcat ttccaaaata tcagcaatgc tttttccttt gtaatgcact tctaatgttt
     6361 cagaattata tcgtttacca tgacatactt cacaaggaac atagacatca gtcaaaaagt
     6421 gcatttcgat cttaataatt ccatccccgc gacaagcttc acaacgaccg ccttttacgt
     6481 tgaaactaaa acgccctttc ttataacccc gcatttttgc ctcgttcgtt tgagcaaata
     6541 acccacggat atcatcaaat acacttgtat aagtcgctgg attacttctc ggcgtccggc
     6601 cgattgggct ttgatcgata tcgatgatct tttcgatact ttcgtagccg gaaattgtct
     6661 tgaatttacc tggcttagca gaattcttat ttaatttttg cgctaacgat ttctttaaga
     6721 tactattgac caatgtactc tttccagaac cagacacacc tgtgactgct acaaattctc
     6781 ctaatggaaa ttcaacacta atatttttca gattgttttc tgaagcacca gtgatcttga
     6841 ttgctttccc attaccttta cggcgttctt ttggtacagg gattgatttt ttccctgaaa
     6901 gatatttccc tgtcaaagaa ttttcatcat tagctacttc ttcaggagtg cctgcagcca
     6961 cgatttgtcc gccttgttct ccggccccag gtccgacgtc aatcaaataa tctgctgccc
     7021 acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt gccaagatca cgcattttct
     7081 ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa tccgatcgat ggttcatcta
     7141 aaatatacaa aactccagaa agattcgatc caatttgtgt agccaatctg attcgctgcg
     7201 cttctccccc agataatgtt ccggaagcgc gactcaaagt tagatagtct agtcccacat
     7261 tttccaagaa tgaaagtctg tcatttactt cttttaaaat cggtttggca attactttct
     7321 cttgttcaga taacgttaag tttgagaaga aatctactgc tttttgaata gacaactcat
     7381 ttgtttgccc gatattcgtt ccattgattt gaacagatag agcttgggga ttcaatcgat
     7441 aacctttaca tgtctgacaa gttaattctg tcatataaag acgcatttgc tcacgcgtga
     7501 aatcactgtt cgtatcatga taacgacgct cgatattctt caatacccct tcgaaagtcg
     7561 tttctacatc gcgaacgcca ccaaaatcat tttcataatg gaaatggaaa agatcatctg
     7621 tcccattcaa gatgatctcc tgatgctctt ccggtaattc ttcgaatggt gtatctaaat
     7681 cgataccgaa actttcacaa gcctgcgcta acatttgagg ataatactga gagctgattg
     7741 gattccatgg aacgatagca ccttgactaa gtgtcttcgt tctatctgga atcactaaat
     7801 cttgatcgac ttctaatttg acacctaaac catcacagtc tggacaagct ccaaaaggcg
     7861 cattgaatga gaacagtctt ggttctagtt cgccaacagt aaatccgcaa taaggacagg
     7921 catagtgttc gctaaacaac atttcttctt caccgatcac gtcaaccaga gcatagccgt
     7981 ctgctaatcg cagggccgcc tctaaagaat caaataggcg agaacgaatc ccttctttca
     8041 caatgatccg gtcgatgacg atcgcgatat catgtttttt gtttttctcc agctcaggca
     8101 cttcacttaa atcatagatc tcaccatcga ctcgaacgcg gacgtatccc tctttttgga
     8161 ttcgttcgaa tattttttta tgttgtcctt ttttctttac cacgatcggt gccaacactt
     8221 ggatctttgt gcgctcaggc aaagccatta cttgatctgc catttgatct actgattgac
     8281 tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc tacacgtgca aacaacaagc
     8341 gtaaatagtc attgatttcc gtcacagtac ctactgtcga ccgaggattt ttacttgttg
     8401 ttttctgatc aatagaaatc gccggactca atccgtcgat gctatctacg tccggcttat
     8461 ccatttgtcc taaaaactga cgagcataag cagaaaggct ctctacgtat ctccgctgcc
     8521 cttcagcgta cagcgtatca aatgctaggg aactttttcc agaaccggac aaacctgtaa
     8581 cgaccacgaa tttatctcgt ggaatcgtta catctatatt tttcaagtta tgggcacgtg
     8641 caccatgaat cgtaatttta tcgttagcca tgttgtcctc ctactttgaa gcttttaatt
     8701 ctaaaatcat atctcgcaca ttcgcggctg tttcaaaatc caaagctttt gccgcatctt
     8761 tcatttcttt ttctaatttc atcaataacg tgtctttttc ttccttcgtc atttcttcgt
     8821 acgagacttg taccgcttct tttgtatcat cttccgattc tttcgtaata gaaatcaaat
     8881 ctcggatttc tttaatgatc gtttttggaa cgatcccatg ttcttcattg tacttctgct
     8941 gaatcgtacg acgtcttgac gtttcatcca tcgctagacg catagaatct gttactttgt
     9001 ctgcgtacat gattacttta ccttcttcat tccgagcagc tcgtccaatc gtttggacta
     9061 aggaacgttc actacgtaaa aagccttctt tatccgcatc caagatagca acaagggaaa
     9121 cttcaggtac gtctaatcct tcacgaagca aattgattcc aactaacaca tcaaattcac
     9181 ctaatcgaag atctcgaatg atttctgttc gttccagcgt tttgatatca ctatgcaaat
     9241 atttgacttt gatccctaat tctttgaaat aatctgtgag gtcttctgcc atttttttcg
     9301 tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg ttcatttatt tcgcctacta
     9361 gatcatcgat ttgtcccatg attggacgaa cttcgatcac tgggtcaagc agccctgtcg
     9421 gacggatgat ttgttggata acggtatctg tttgttcatg ttcatatggt cctggcgtcg
     9481 cagagacata aatgatctga ttgacatgtt tttcaaactc ttctaaccga agtggccgat
     9541 tgtctaaagc agatggcaaa cggaaaccgt aatctactag catctgtttt ctcgctcggt
     9601 caccattata cattcctctg atttgaggca tggttacgtg cgattcgtcc gccacgatca
     9661 aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg ttcaccttct ttacgtccgt
     9721 ccatatgccg agaataattt tcaattcccg acgtgtagcc catttctaac atcatttcaa
     9781 tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag aagtttgttt tcatttcgca
     9841 agacagttaa acgctgttct aattccgctt tgatattcgc tacggcatgc tccatatgtt
     9901 catcattcgt cacaaagtga gtcgccggga agattgctac gtgttccgtt tctcctaaaa
     9961 cttcacctgt cagtgcattg acttctcgga tacgttcgac ctcatctcca aaaaactcca
    10021 cacgtaaggc acgttcatct cttgaagccg gaaaaatctc cacgacatca ccgcgcactc
    10081 gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa ttggatactg actaagtcac
    10141 gaatcaactg attgcgatct agttctgctc cctgtcgaat cgaaactact tgtttttggt
    10201 actcaaaagg cgagcctaat ccaaaaatac atgaaacgga tgcaatgacg atcacatcat
    10261 ttctttctaa taatgaacta gtagccgaat gtcgtaattt gtcgatctca tcatttacac
    10321 ttgaatcttt ttcgatatac gtatcgcttg aaggcacata agcttcaggc tgataataat
    10381 cataataaga aacaaaatat tcaacagcat tgttagggaa aaattccttg aattccccgt
    10441 acagctgacc agccaatgtt ttgttatggg caataatcag tgttggttta ttcactttct
    10501 caataagatt ggaaatcgta tatgttttcc ctgttccagt cgcacctaat aagatttgtg
    10561 cttttttccc gccgactacg ccatccacta attggttaat cgcttcaggc tgatcaccag
    10621 ccggttggta tttcgagaca agctcaaagt gtcgggaggt atctctctca atcattcttc
    10681 gtcctccttt tcattcattc cttttcattt tacgaccaat aaaaagaaaa ggaaagcaaa
    10741 ggcattcctt actaaaaaat tataccacac cgaacatact ttcgccactt ttttatctta
    10801 ttatacatgt tatgttttat aacataaaat gttagaaact cgcatttttt tcattttttc
    10861 gagcttacag tcttgtctga ttcagcactt ttcgcctata ataataaaat attggaaatt
    10921 taggaggttg attatgaaaa gaaagcattt atttcactca tttgttttct taatgacagg
    10981 acttgtgaca cttgcgtttg gtcatgttgc acaagctgac aacactacaa cggcagagtc
    11041 aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg taattgccag
    11101 tgactctgct tttgctccat ttgaatttca aaacgcaaag ggcgagtacg aaggcatcga
    11161 tgtaaaattg atggaaaaca tcgccaaact gcaaggattt aacatccgaa tggactaccg
    11221 tggtttctct gctgctttgc aagctttgga atcaggacaa gcagacggaa tgattgccgg
    11281 aatgactgta acagatgaac gaaagaaaag ctatgatttt tctgattctt actttgatag
    11341 cgggatccaa ttagctgtca aaaaaggaga tacttcgatc aagtcttatg aagatttgaa
    11401 aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct gaatttttaa gcgaccatga
    11461 atctgaatat ggctattcta tcaaatattt tgatgctgct gatcaattgt acgacgggtt
    11521 aaaagttggc gcaatcgatg caatgatgga tgattatccg gtaatcggtt atgcgatcaa
    11581 acaaggacaa gaattggaaa caccgatcaa aaaagaatct ggcggaaaat atgcatttgc
    11641 cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc aatgaaggat tgaaagaatt
    11701 aaaaagaaca ggcgaatacg ataagattat taaagaatat gtagcggatg gttcagaaac
    11761 ttccagtgag tcttccactg ctgatgaatc aacatttctt ggacttatcc aaaacaacta
    11821 tcaagcattg ttgaaaggac tttggagaac aattctccta actttgatct cctttgcttt
    11881 ggctatagta atcggtgtga tttttggatt attcagtgta gcaccaatac gcgctcttcg
    11941 cacaattgct agtatttatg tcgatatcat ccgaggaatt ccaatgatgg ttcttgcgtt
    12001 ctttattttc tttgggttgc ctggtatcgt tggatttacg attcctgact tccttgctgg
    12061 tgttatcacc ttgactctta acgcaagtgc ttatatcgca gagattgttc gaggcgggat
    12121 caacgccgtt cctgtcggac aaatggaagc ttccagaagt ttaggtcttt cttataatcg
    12181 gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc atgatcccat cattcgtcaa
    12241 tcagttcgtt atctcattaa aagacacaac gatcatttcc gcaatcggtg tagtcgaatt
    12301 gctgcaaact ggtaagatca ttgttgcacg tacaacacaa agtacgtatg tttacttgat
    12361 cattgcgatc atgtacttga ttttgatcac agcattgaca aaattagcaa aagttttaga
    12421 aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga gcatttagtc aaaaaatacg
    12481 gcgataacac agtattaaac gacatcaatg tatccatcaa cgaaggagat gtcgtctgta
    12541 tcattggccc ttcaggttca ggaaaaagta cattcttgcg ttgcttgaat cgtttagaag
    12601 aaccttctga cggtgaaatt attattgacg gcgcccactt gatggataaa agcacagata
    12661 tcaacttggt gagacaacat atcggaatgg tattccaaca ctttaaccta ttccctcatc
    12721 tttctatttt ggaaaacatc attttagcac cgatggacct gaagaaagaa tctagagaag
    12781 aagcagagaa aaaagcaatc aaattactgc agacagttgg tctagaagat aaaaaagata
    12841 tgtatcctga aatgctttct ggtggacaaa aacagcgtgt ggctattgct cgtgctctag
    12901 cgatgaaccc tgacatcatg ctttttgatg agcctacttc tgcactcgat cctgaaatgg
    12961 tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg atggttatcg
    13021 ttacgcacga aatggggttc gctaaagaag tggccaatcg cgtcatgttc attgacgacg
    13081 gaaacttttt agaagatgga acgcctcaac aaatctttga aaatccgcaa aatgaacgga
    13141 caaaagattt cttgaataaa gtattaaata tttaataaaa accaaaaatg cacggtcgcc
    13201 atgaagggac cgtgtatttt tatcttctta ttttctcacc taatcgtatc agccgcatcg
    13261 cttgttttcc tgcacaaaca agcaatcgct ctacctgttc catcgcataa gaaagtacca
    13321 tcggttcatt gatgatatca agtacatagt cgataccacc ctcacttaat tgagcttgat
    13381 ttttcttctc catattatgt agttgtggga caactttctg acaatcagca attgtgtcaa
    13441 gaacggatca atcctttatt ggagttgctc gaaagggtct tctcttatta cggacaggct
    13501 ttgctcactg tacatagaca acaaatcatt atcctagtca atcggatttc cagagcctca
    13561 ttgctatcat tgcttgataa aatccagaca aagtttaata aaatgtatca gttacagcta
    13621 aactttggaa ttgggtcgct ttgttataca gagcaagaga caccgcaatc tttccttcat
    13681 gccaaacaag tttgcgaatg gatagctttc catcaatctg tgaacgagat ccgctttttt
    13741 gaagatttag acctcgggat cgttttacca gcaatcccgt cagatcaacg aacattatat
    13801 gtaaaacgta ttttgaaaag cctgacagaa gaagaagtac atttgttcaa gaagactttg
    13861 gcatgcttca gtaaaaataa cggaagcatc aaaaattgct cagaagaatt atttattcat
    13921 aaaaacacct tgcaatatcg attgaacaag ttccattctc tcactggtta tacccctaga
    13981 aactatgacg attatcatat tctaaagctg gct
//
LOCUS       DAFBYI010000068        13977 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFBYI010000068 DAFBYI010000000
VERSION     DAFBYI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.068"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LLD04_001822"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LLD04_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK5648749.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LLD04_001823"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LLD04_001823"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK5648750.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LLD04_001824"
     CDS             complement(2169..2906)
                     /locus_tag="LLD04_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5648751.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LLD04_001825"
     CDS             complement(2997..3524)
                     /locus_tag="LLD04_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648752.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LLD04_001826"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LLD04_001826"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK5648753.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LLD04_001827"
     CDS             complement(4401..4886)
                     /locus_tag="LLD04_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK5648754.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LLD04_001828"
     CDS             complement(5039..5236)
                     /locus_tag="LLD04_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648755.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LLD04_001829"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LLD04_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK5648756.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LLD04_001830"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LLD04_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK5648757.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LLD04_001831"
     CDS             9211..9423
                     /locus_tag="LLD04_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK5648758.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LLD04_001832"
     CDS             9425..11104
                     /locus_tag="LLD04_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5648759.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LLD04_001833"
     CDS             complement(11358..11558)
                     /locus_tag="LLD04_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5648760.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LLD04_001834"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LLD04_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="LLD04_001835"
     tRNA            complement(12249..12322)
                     /locus_tag="LLD04_001835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="LLD04_001836"
     CDS             complement(12634..13032)
                     /locus_tag="LLD04_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648761.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="LLD04_001837"
     CDS             complement(13025..13477)
                     /locus_tag="LLD04_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648762.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="LLD04_001838"
     CDS             complement(13464..13970)
                     /locus_tag="LLD04_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648763.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4116 a         2943 c         2348 g         4570 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcacta
//
LOCUS       DAFBYI010000069        13917 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFBYI010000069 DAFBYI010000000
VERSION     DAFBYI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.069"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            131..505
                     /locus_tag="LLD04_001839"
     CDS             131..505
                     /locus_tag="LLD04_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5648764.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LLD04_001840"
     CDS             complement(619..1248)
                     /locus_tag="LLD04_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5648765.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LLD04_001841"
     tRNA            complement(1382..1454)
                     /locus_tag="LLD04_001841"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LLD04_001842"
     tRNA            1668..1741
                     /locus_tag="LLD04_001842"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LLD04_001843"
     CDS             complement(1879..2043)
                     /locus_tag="LLD04_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648766.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LLD04_001844"
     CDS             2338..3666
                     /locus_tag="LLD04_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK5648767.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LLD04_001845"
     CDS             3868..4701
                     /locus_tag="LLD04_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK5648768.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LLD04_001846"
     CDS             4717..5454
                     /locus_tag="LLD04_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK5648769.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LLD04_001847"
     CDS             complement(5609..6577)
                     /locus_tag="LLD04_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK5648770.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LLD04_001848"
     CDS             6809..7642
                     /locus_tag="LLD04_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK5648771.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LLD04_001849"
     CDS             7676..8515
                     /locus_tag="LLD04_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5648772.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LLD04_001850"
     CDS             8541..9101
                     /locus_tag="LLD04_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5648773.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LLD04_001851"
     CDS             9300..11021
                     /locus_tag="LLD04_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK5648774.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LLD04_001852"
     CDS             11186..12328
                     /locus_tag="LLD04_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5648775.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LLD04_001853"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LLD04_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK5648776.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2298 c         2833 g         4034 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAFBYI010000070        13656 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFBYI010000070 DAFBYI010000000
VERSION     DAFBYI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.070"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(146..1111)
                     /locus_tag="LLD04_001854"
     CDS             complement(146..1111)
                     /locus_tag="LLD04_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK5648777.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="LLD04_001855"
     CDS             1345..2190
                     /locus_tag="LLD04_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5648778.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="LLD04_001856"
     CDS             2381..3568
                     /locus_tag="LLD04_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5648779.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            3574..3930
                     /locus_tag="LLD04_001857"
     CDS             3574..3930
                     /locus_tag="LLD04_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK5648780.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="LLD04_001858"
     CDS             3932..4270
                     /locus_tag="LLD04_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK5648781.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="LLD04_001859"
     CDS             4665..5699
                     /locus_tag="LLD04_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5648782.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="LLD04_001860"
     CDS             5692..6378
                     /locus_tag="LLD04_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648783.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="LLD04_001861"
     CDS             6402..7250
                     /locus_tag="LLD04_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5648784.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="LLD04_001862"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="LLD04_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK5648785.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="LLD04_001863"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="LLD04_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK5648786.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="LLD04_001864"
     CDS             9516..10751
                     /locus_tag="LLD04_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5648787.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="LLD04_001865"
     CDS             10738..11208
                     /locus_tag="LLD04_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK5648788.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="LLD04_001866"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="LLD04_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK5648789.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..>13656)
                     /locus_tag="LLD04_001867"
     CDS             complement(12699..>13656)
                     /locus_tag="LLD04_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5648790.1"
                     /translation="KAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILPAFGK
                     LKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTLLPRK
                     KEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKDIDTF
                     NKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYLKLGY
                     NTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLEAGES
                     VKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4464 a         2539 c         2967 g         3686 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 gatgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgta agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttc
//
LOCUS       DAFBYI010000071        13455 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFBYI010000071 DAFBYI010000000
VERSION     DAFBYI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.071"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..430
                     /locus_tag="LLD04_001868"
     CDS             <1..430
                     /locus_tag="LLD04_001868"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK5648791.1"
                     /translation="TIDAKHRLNMLELAVEDNPFLQIETIELARGGKSYTYDTMKELT
                     QNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGYTTDTPYPVIWVDVPE
                     IDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            414..1007
                     /gene="yqeK"
                     /locus_tag="LLD04_001869"
     CDS             414..1007
                     /gene="yqeK"
                     /locus_tag="LLD04_001869"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK5648792.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            1064..1387
                     /gene="rsfS"
                     /locus_tag="LLD04_001870"
     CDS             1064..1387
                     /gene="rsfS"
                     /locus_tag="LLD04_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK5648793.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            1391..2131
                     /locus_tag="LLD04_001871"
     CDS             1391..2131
                     /locus_tag="LLD04_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5648794.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            2348..3499
                     /locus_tag="LLD04_001872"
     CDS             2348..3499
                     /locus_tag="LLD04_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK5648795.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            3630..4349
                     /locus_tag="LLD04_001873"
     CDS             3630..4349
                     /locus_tag="LLD04_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5648796.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            4528..5175
                     /locus_tag="LLD04_001874"
     CDS             4528..5175
                     /locus_tag="LLD04_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648797.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(5268..7547)
                     /locus_tag="LLD04_001875"
     CDS             complement(5268..7547)
                     /locus_tag="LLD04_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5648798.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            7825..9648
                     /gene="cadA"
                     /locus_tag="LLD04_001876"
     CDS             7825..9648
                     /gene="cadA"
                     /locus_tag="LLD04_001876"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5648799.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            9695..9874
                     /locus_tag="LLD04_001877"
     CDS             9695..9874
                     /locus_tag="LLD04_001877"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK5648800.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            10211..12151
                     /gene="thrS"
                     /locus_tag="LLD04_001878"
     CDS             10211..12151
                     /gene="thrS"
                     /locus_tag="LLD04_001878"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK5648801.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            12375..13223
                     /locus_tag="LLD04_001879"
     CDS             12375..13223
                     /locus_tag="LLD04_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5648802.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         4355 a         2337 c         2890 g         3873 t
ORIGIN      
        1 gacgattgat gcaaaacatc gactcaacat gttggaatta gcggtggaag acaatccttt
       61 tttgcagatt gaaacgattg aattagctcg tggtgggaaa agttacacct atgatactat
      121 gaaggaattg acgcaaaaca atccggatac agattattac tttattatcg gtggggatat
      181 ggtagaatat ttgccaaaat ggtataaaat cgatgagctg acttctatgg tcaactttgt
      241 gggtatacgt cgaccaggat acacaacgga tactccatat cctgtaattt gggtagatgt
      301 acctgagatc gacatcagtt ccacgaaaat acgtcaaaag atcaaagaag gctgttctat
      361 ccgctacctt gttccagata aagtaattga ttatattcaa aatgaagggt tgtatgaata
      421 tggactatag taatgaatat actgcttatt ctagagaaaa cctgatgcaa aaaatacaga
      481 tgagaatgag cgaacaacgc ttcaagcatg tgcttggagt agaagaaaca gccgtggctc
      541 ttgccaaaaa atacggtgct tctcctgaaa aagctagtat tgctgcattg acacatgatt
      601 atgcaaaaga acgaccagat gaggagttca aaatggtaat cgtaagagac ggttttgatc
      661 cggagttatt gaattataat aatgcaatct ggcatggtct tgttggcgct tcgtttgtag
      721 agcgggaatt aggaatcacc gatgcagaga ttcttcatgc aatccgggtg catacgactg
      781 gggctgctaa gatgagtctc cttgacaaaa tcatttatgt agcagactat attgaaccag
      841 gtcgagattt tcctggggta caagatgcac gagccattgc atgggctgat ctagacgaag
      901 ctgtagcatt tgaaacaaaa catacattgg cccatttgct tgcgcaagaa caacagattt
      961 atccaaaaac aatcgaaaca tataattact gggtagcaaa aaaataagct gctcatatta
     1021 ggaggaaatc atcatagata gtaaagaaca attaaaaatt gcgattgaag cagcagattc
     1081 aaaacgggca gaagacatta tcgctttaga cgttcgaaat gtttccttgt tggcagatta
     1141 tttcatgatc tgttcagcaa atagcgaacg tcaaatcaac gcaattacag aagaaatcat
     1201 tgataaagaa gaagaaaaca aatatgaagt aaaacgtatt gaaggaaaag aaggcggaaa
     1261 atgggtattg atcgatcttg gagatgtgat cgtacatgtt ttccatgcgc cagaacgaaa
     1321 tttctataat ctggaaaaac tatggtcaga tgctccgcta gtcgatctta gtgaatggtt
     1381 ggattaacat atggcttatg aaacatttgc tttcatttac gatgaagtga tggacgaaac
     1441 actttatcag caatggttag ctttttcgaa acgtcattta caagaagaca caaaaaatat
     1501 tttggaactg gcatgcggta ctggagcatt agcggttgcc tttgcaaaag atggatataa
     1561 agtgacaggg ttagaccttt cagaagagat gttgatgatt gccagccagc gagcatatga
     1621 agaagacgca tcgatccaat ttgtggaagg caatatgctc gatttatcag aaatcggaca
     1681 atatcaggca attacctgtt tttctgattc gctgtgctat atgaaaaata gacaagaggt
     1741 acagcaagtg tttgatgaag tctatcaagc gttagaagaa aatggccgat ttttatttga
     1801 cgtacactcc acctatcaag tagatgaggt ttttccagaa tatagctatc attatcaagg
     1861 ggataatttt gcttttttgt gggatagtta tccaggaagc gagaaacaca gcatcgaaca
     1921 ttttcttacc tttttcgttg aagaacaaga agggtctggt cgatttattc gtgaagatga
     1981 actacatcaa gagcgtacct atccattgga aagttattta agaatgctgg aaaacagcgg
     2041 cttttcgaaa gtcgaagcat tttctgattt tacagacgaa gcagcatcag agaaatcaaa
     2101 gcgctggttc tttgtagcat acaaagacta gcggttttgc ctgctcgagt taacaagaaa
     2161 agaataaatc ttcaacataa attatatatc cgaacgatat aggcattgct cttgcagttc
     2221 agcttttcgt cgcagaagta ttgtgtttgc gagaatagtt tatatagttc gggtattttt
     2281 tccgattttt gccttttttt acttgtctct atttcttttt tatcgataac tatgaaggag
     2341 gatgcgcatg aaaagctgcg ggattatcgt agagtacaat ccttttcaca atggacatcg
     2401 ctatcatgtg gaaatggcac gaaaaataac aggagcagag gtcgtcattg ctgtaatgag
     2461 cgggaatttt ttgcaaagag gcgaaccggc tatcatcgat aaatggcatc gcgcgaatga
     2521 agctttacaa aatggtgtgg atttagtagt ggagcttccc gtcgaatggg cggttcagtc
     2581 agctgactat tttgctaaag gtgcagtaaa aatcttgcaa acactggact gtgacagttt
     2641 gtgttttgga acagaagaag agacttcgtt tgattacagc agtttcggac gctttgtcca
     2701 agagaatcaa gataggatag aagaggcatt tcaagaactg tctgatcaaa cgtatagtta
     2761 tccgcaaaaa atgactgagg tttttcgcag tctgtatccc gatattacgc ttgatttttc
     2821 ttcgccaaat catatcttgg gattaagtta tgcaaaggaa aatgccaagt atgtccaccc
     2881 tatggaatta catccgatcc gacgtaaaag tgcaggctat catgaaaaaa tcatcacaga
     2941 tcagaggata gccagtgcaa cagccattcg ccatgcgtta gaagaaggaa aaagaatagc
     3001 tggacttgtg ccgcctcaga cagaagaaga tctttcaact tgttcgctgc agacttggga
     3061 atcttattgg ccgtatttgc gttatcggtt gttatcttct actttgctgg aactgacaga
     3121 tatctatcag atgaccgaag gaatagaatc gcgcatgaaa acagcagctg caactgctga
     3181 tagttttgag gattttgtgt caaaggtaaa aacaaaaaga tatacatgga cacggatcca
     3241 acgattatct tgttatgtct tgctgaatat caaaaagcaa gagattcctg tccaacaaaa
     3301 taaaaattat cttcgactac ttggatttac cgatgctgga agaagattct taaaagaaaa
     3361 aaaagagatt tctatttatt cccgaatcgg aaaaaaagag gcacaagaag cggaactgtt
     3421 agttagaagt gatcaaatct atcaattggg aggcaatatt ccggaacaga atttcgggca
     3481 aatcccaatc cgagtataga taattgacta aaaatgagaa gaatgaagga agaaagaaaa
     3541 aaagcttcac agaatcgtga ttcacaagta taatggaaca ggacgttttt gcgttatgaa
     3601 actatcaaat cagaaagcga agtgaaaata tgggacgtaa atgggcaaat atcgtagcaa
     3661 agaaaactgc taaagatgct aataatagcc ggatttatgc aaaatttggg attgaaatct
     3721 acgcggcagc aaaatcaggt gatcctgatc cgcatgcaaa ccaaaaacta cgtttcgtca
     3781 ttgatcgcgc taaaacttat aatgtaccaa aacatattat tgaccgagca atcgaaaaag
     3841 caaaaggtgc aggagatgaa acatactctg aattgcgcta tgaaggattt ggaccaagcg
     3901 gctcaatggt gattgttgat acattgacga acaatgtgaa tcgaacagca gcagacgtac
     3961 gggcagcatt tgggaaaaat ggcgggaaca tgggtgtttc cggagcggtt tcttacatgt
     4021 ttgacaatac agcaatcttt gcttttgcag gagaagacgc tgacgagatc cttgaatacc
     4081 taatggaaaa agacatcgat gttcgcgatg taatggaaga agaaggacaa atcatcgttt
     4141 acggtgaagt agcggatttc catagtatac aagaagcatt aaaagaaaaa ggaatcactg
     4201 atttttcaat cgctgagatc caaatgatcc ctcaaaacga ggtaacacta tctccagaag
     4261 atcaagaaac atttgaaaaa atgattgatg cactggaaga tctagaagat gtacagcatg
     4321 tgttccataa tgtggcatta gaagactaag caaccagttt taacgaaaag tattcagtct
     4381 ttttcgaaaa gcaagacaat ctcactaaca gattgtcttg cttttttgtg tgatttattt
     4441 tcttttctaa ttcaacctgt ggtatgatga gagcataagt atcatgtgaa atgatatgaa
     4501 gtatgtggaa gatggacggg gaagaatatg gaagcaaaaa tcactttaga accttttgaa
     4561 cgtatcttaa gcggttaccg caaagtagaa gagttagctg tgaacgtaac tgattgttca
     4621 aaattagcgc aaaaatacgc aagattcggc gttgaaggct atcgattagg aaactatgtc
     4681 ggtacagggt atctgaaccg ttatttagag tgtatggtgg accgcgcacc aatgctgatt
     4741 tatagacaaa attatctgat tccgctgctt tttcgtcgtt ctgattcagc ctttcggtta
     4801 tttgaagaag agtatcggat ggaggctttc tttctactat tagagtggag cttgaaacat
     4861 cgtcctgaaa aaattcttat cgaaagaaat gagaagatag acacgaaaaa aaataaagtg
     4921 atcgatagtg catacctagc atttcgtgta tcagagattt tggattgtgg cggctatcca
     4981 atcagcaatt tccaatcaat tgaccaattc attgaatgga atcgaattta ccgattgatc
     5041 gacaatggcg ggattggccg tcatagcaag gtatttgacc cagaatatcc agaaaacatg
     5101 gaagaactga aaatgattat ttcgcttgtc aaattaaaat atcctgaaac agatttggat
     5161 ttatatatag aataaatgac cttaaatgat tgaattactt tttctgcaat tattattgta
     5221 aataaaaaga caactgctaa cattaatgat agcagttgtc tttttactta cagtactttt
     5281 ttctcataag atgaaatatc catattgttt aacaaaggac tttcattatt ggaggtaaaa
     5341 agggtcaatt catgcatcgc acgtgtacac atggtgtaaa ggatcaattt gtcttgctga
     5401 gaagaaaaat gttctgcatc tacatcccac aagaaaactc ggtcaaactc cagacctttt
     5461 gctaaaaagg ctggaagtaa aataatggaa cgcttcataa agtcactctc atttaaaatc
     5521 agctggactt gttgtttcaa ctcgtctggc agttgagcat aaagagcttc gcattcttct
     5581 tgtgttttag ctatgattgc tgtacgccaa taccgggctt ttccctgagt cgttcgaatg
     5641 gtatctgcta accaattcaa tttgatatca tctgttgttc cttggatgag caaaggaagg
     5701 tctccttttc ttgctgttgt ttctacttga tgttctttca caataaaatg attagcaaaa
     5761 tcagtgattt cttttgtgga gcgatagctt gtcgttaatt ggaaacgagt gacttcacgg
     5821 tccacgaaaa ggtcttccaa tgatccaacg atcgtttcat ttccaaagac attttgattt
     5881 agatctccac agaaagtcaa atttgctttt gggaacaagg cttgcagcaa tgccacttgt
     5941 gatggtgcga aatcctgcat ttcatcaata aagatatagc gagctttgtg tgtaaccgtc
     6001 actggatatg ttcggcgcat cagtaaaaag aatagtacag catcctcttg acgcaattct
     6061 ctttgacgca agtgtgtacg aacagttgtc aaatgttcct gccacctggt ctcgctgatt
     6121 ttatgtcgtg ttagaagaga aagtggcaca gtttgtagaa agtggagata ttgtttcgtt
     6181 atattgacaa attgataatt catgatccct cgatggattc gacggaatct tttacgtacg
     6241 atttggttgg ccagtttttt acgtaatgaa cgctcctttt gttctgtata ttcaagattt
     6301 ggatctgttg cgtatagttc ttggagctgt tcttccgcca gatctttgac ccattgctga
     6361 cgtgtttcgt ctttttgcag accgcctaat tttttcagga gcttcgtttg taatagagaa
     6421 agtctatgat gtaaaggtaa ttgttcattt gtttctttat accattggcg aatcgattcc
     6481 tttgatagga tcgtacggcc atttattttc atatcacgga acaagggacc gtagtttgtg
     6541 atgccttgga tatagcgatc aatctgatcg actaaggtca atccgctttt catcacttgt
     6601 atcggatctt tttcccctag caaaaaacca ctttcttgtt gctgttcatc tagtaaagaa
     6661 aactctggca gcagctgcac caaataattt ttgaatgtct gtgtaggcac gccgctttca
     6721 cctaatgaag gcaatacagt cgagatgtaa tcagaaaaca aatggttggg tgaaaataga
     6781 agaacgtttt cagcctcgag ccacttgcga ttatgataca acagaaaagc aatacgttga
     6841 agcaaagcgg atgtctttcc acttccggcg attccttcaa tcagcattac tttacttgtt
     6901 gtgtcccgaa tgattgcatt ttgtgctttt tgtatcgtag agacgatgtt tttcatatgg
     6961 ttgcttgatg attcatccaa tatctctagc aaaaagtcat cattgattac ttccgacgta
     7021 tctaccatag ataaaatacg tccttcttgg attttaaatt gtcgtttcaa ctgcagatcg
     7081 acatctattc tatctgtttc agtttcataa aatgcgggac caatctcacc ttcataataa
     7141 agattggcaa ttggtgcgcg ccagtcaata acgattgttt cttctgccag atctcttaaa
     7201 gaagcgatac ctagatataa tgtttctgtt tcacggtctt ctttaaagtc gatacgggca
     7261 aaataaggac tatctttcat gacagacaac gtttgcaggc gtttttgctg tgcttcaaga
     7321 gtatggtatt tcaaaagcaa ttcctgctca tgttggcgat attcaacagc agattcataa
     7381 aaagcttcat tggaaccagc tcgaatcttg tgattagctg tgtcttttgt cgaatgattc
     7441 atttcctgag tcaattcttg ttgttttgta tccagcatat tttgttcgat ctggattagt
     7501 tcgatcgttt catccacgtg cgtttgttct agttgtttct cattcatcgc atgtttgctc
     7561 cttccgccgt aaataacgaa ttaaaagtat agcatagttt ttcgaaaaaa ggtacttttt
     7621 aactgatgaa tcgaaaaagt aatccttgct gaatattttt gtttgtagaa ctgatgaatt
     7681 ttggaaacgc tgatagtgat agttgttttc aattaaggcc tattgtttta aaaagtgcta
     7741 gtcaaggact atgcttaaga tgaaagaggc agctggaaat ttattactaa cctctctaag
     7801 tcacataaaa gaggagaaaa aacaatgaca catttcaaaa aatttttact tactttgctt
     7861 attggtggga tagctttatt aagtgaattt gtttttcacc agccggccct tgcatttgcg
     7921 attgtcgcat ttactggagg gattctcgcc ttcttgatgt ttttggaaat gatcaagacg
     7981 ttacgttcag gaagatatgg cgtagatgta ttagcgatta ccgcaatcct tgcaacatta
     8041 gccgtcaaag aatactgggc tagtttgatg atattgatca tgcttacggg cggagatagt
     8101 ctagaggatt acgcgaataa acgagcgggt aaagaactgc agtctttgtt agataacacc
     8161 ccaagaattg cccatcgatt caaagaagga aaattgcagg atatagtaat cgatgaaata
     8221 aatattgatg accagttact agtcaagcct ggcgaacttg tacccgcaga tggtcgagtg
     8281 accaaaggag aatcttcggt cgatgaatct tctttgacag gtgagtcccg cccggaaacc
     8341 aagaaacctg gagatcagat tatgtcaggg tctttaaatg gagacggatc gatccaaatg
     8401 atcgctgaaa aacgtgccgc agacagccag tatcaaatga ttattaaact agtcgaagaa
     8461 tcaaaagaaa agcctgcccg atttgtccgt ttagctgatc gttatgcagt ccctttcacg
     8521 ttgattgctt atttgatcgg tggtatttct tggtggttct caaaagaccc tttacgattt
     8581 gctgaggtac tggtagtggc atcaccttgt ccactgattc tagctgcgcc cattgcttta
     8641 gttgcaggaa tgagccgttc aagtaaaaac gggatcgttg taaaaactgg aacaacgatt
     8701 gaaaagctcg cacgtacaaa aacgattgct tttgataaaa caggaacgct taccaaaggg
     8761 atattggaag tagaggcgat tcagccagtc aatggaataa aaaaagaaga gctacttctg
     8821 tatgcagcta gtgcagaaca ggaatctagc catatcttag ctcgttcgtt agtgaacaac
     8881 gtgaaaaaca gccaactgta tcctgttagt caactgaaag aagtgaccgg tcaaggaatc
     8941 caagcagtag tcaataatca tgaagtaaag attggacgtg cctcttttgt ttcagctgaa
     9001 gaaatagaaa cgtccaatac agcaatctat gtaacaattg atcgatcttt tgcaggaacg
     9061 atcctatttt cggatcagat tcgcccagaa gcagcaaaga caatcaagga gctaagaaaa
     9121 cttgatgtga aaaatttgat gatgatcacc ggggatggat ctgccattgc tcaaacgatt
     9181 gcagaaaaag tccaattaga ttctgtacat gcccgtagct tgcctcaaga taaactgacc
     9241 attttagaaa gtatccctaa agatcaaaga cctgttgcga tggtaggaga cggcgtgaat
     9301 gacgcaccag cacttgctgc tgcagatatc ggtattgcaa tgggggctca tggttctaca
     9361 gctgctagtg aaagtgcaga tgcggtcatc ttgaaagatg atctcttgcg tgtagcaaaa
     9421 gcagtagtga tttcaagaga tacgatgaga atagcgaaac agtctgtact gattggcatc
     9481 tttatttgta tttttttgat gttaattgca agtacgggac taatccccgc attattcgga
     9541 gctgttttgc aagaagtcgt ggatacggtc tctattttga gtgccttgag agcaaaaaaa
     9601 gatccagcca tttttcctga ttcagtaccc aagacaaaca tagtatgata gaatcgagtt
     9661 gaaaaaagaa cgatcagaaa gaggagagag cattatgcca ttcgtacatg tcgaattagt
     9721 agaaggtcgc agtccggaac aattagaaaa tatgatgaaa gatatcacgg aagctgttca
     9781 taaaaacacg caggcaccaa aagaacacat ccatgtcatc atcaatgaga tgaaaaaagg
     9841 aacatacggt gtaaatgggg aatggaaaaa ataagtttag tttcccactt gatttttcta
     9901 tttattagtg gttaaataaa cacataattg attgatgaga aagccaactg aatttgagag
     9961 tcttcgataa gagaggaaaa gctaggctga aacttttcca ggagaaaacc aaaggatgac
    10021 cactttcgaa acccttgttg aagcaagggc ggtcggaccg ttacaacgct tgaggaacgc
    10081 atttgcgttt gaacattagg tggaaccacg aacttttcgt cctagtatct cttttcaaga
    10141 gatgctagga tttttttgtt tttcagcttg atgatctgat caatcaaaac atttcaagga
    10201 ggaaactatt atgataaaaa ttacttttcc agatggtgca gtcaaagaat ttgagtctgg
    10261 aacaacaaca ttggcaattg ctgaaagcat ttcaaaaagc ttagcaaaaa aagctttagc
    10321 tggtaaagtg aacggaaaac tcgttgattt gactcgtcca atcgaggaag atgcaagtat
    10381 tgagatcatc acaccagacc atgaagatgc acttggctta gtccgtcatt cagcagctca
    10441 tttgatggcc caagccatgc gtcgcctcta tccaaatatc cactttggcg ttggaccagc
    10501 tatcgattca gggttttact acgatacaga taatggacaa aaccaagtaa ctgcagaaga
    10561 tttgccggca atcgaagcag aaatgatgaa aatcgtcaaa gaaaatctgc cgatcgaacg
    10621 ccgtgttctt tcaaaacagg aagcattaga aatttttgca agtgatccgt acaaagtaga
    10681 actgattagt gaattgccag aagaagaagt gattactgct tatcaacaag gcgaattcat
    10741 cgatttgtgt cgtggaccac atgttccatc cactggccgt atacaagtat ttaaattatt
    10801 atcagtggca ggtgcttatt ggcgtggaaa ctcaaacaat caaatgatgc aacgtgtata
    10861 cgggacagcc ttttttgaca agaaagcttt gaaagaatat atccgtctcc gtgaagaagc
    10921 aaaagaaaga gaccatcgta aactaggaaa agaacttgac ttgttcatgg tttccccaga
    10981 agtaggttct ggtttgccat tctggcttcc aaaaggggca acaatccgac gcacgatcga
    11041 acgttatatc gtagacaaag aagttagtct aggttaccaa catgtatata caccaataat
    11101 gggagatgtt gagttgtaca aaacatctgg acactgggat cattatcaag aagacatgtt
    11161 cccgccaatg gatatgggag atggcgaaat gcttgttctt cgcccaatga actgccctca
    11221 tcacatgatg gtctacaaaa acacgatcca ttcttatcgt gaattaccaa ttcgtatcgc
    11281 tgaattagga atgatgcatc gctatgaaaa atcaggtgct ttgtcaggtt tacaacgtgt
    11341 acgagaaatg acgttgaatg atggtcatac atttgttcgt cctgatcaaa tcaaagatga
    11401 atttaaacgt acgcttgaat tgatggtggc agtatatgct gacttcaata ttacagatta
    11461 tcgtttccgc ttgagctatc gcgatccaaa caatacagat aaatattttg atgatgacgc
    11521 aatgtgggaa aaagcccaag caatgctgaa agccgctatg gatgaattag aactagatta
    11581 ctttgaagca gaaggcgagg cagcctttta cggaccaaaa ctagatgttc aagtcaaaac
    11641 agctcttggt acagaagaaa cattatcaac tatacaatta gacttcttat tgccagagcg
    11701 ctttgatttg acatatgttg gagaagacgg ggaaaatacg catcgtccag tagttatcca
    11761 ccgcggaatt gtttctacaa tggaacgttt tgtcgcttat ttaacagaag tatacaaagg
    11821 cgcttttcca acatggttgg cacctataca agcaacaatc atccctgttt ctgttgatgc
    11881 acatggcgat tatgcttatg aaatcaaaga acgtttgcaa atgaaggggt tgcgtgtaga
    11941 agttgatgat cgtaatgaaa aaatgggcta taagatacgt gcctctcaaa cacaaaagat
    12001 cccttaccaa ttagttgttg gagacaagga attggaagat gcaacagtga acgtacgtcg
    12061 ttatggaagt aaagaaacag ctgtagaaga tttgaatatc tttattgatg ctatggaagc
    12121 agaagtcaaa aattacagca gagaaaacta ataggtaaat ttcctgtaaa aaagtagtag
    12181 atagaaagca atccgaacaa tagataacag gtattcctca aagacacaga tgctcaatag
    12241 aatctgtggg attgatggga taagatattc tgttgggcgg attgtttttt tgttttttat
    12301 ccagtatacg accgtgaata agggtctgat atggtatact tacatagact atcattaaaa
    12361 gggtggagga acttatgagc gtaattagtc gtattgaaag tatgatggaa gattattctt
    12421 cagcagaaaa aaagttggcc aactacatta tcggtaatat tgagaaggtc cctacaatga
    12481 cagctaatga attagcagaa gcttccgggt tgagtgcgcc gacagttgta cgtttttcca
    12541 aaaaaatcgg ttttcaaagt ctgacggact tcaaaatcac gatttcaaca gaactacaga
    12601 ctggaatcga tgaaggtttt agtgatatag aagcaaatga gtccttttac tctatcaaaa
    12661 acaaacttgg taacaatgca caagtagcga taaaagaaac agtagatatt ctagaagaag
    12721 aaacgatcca aaaagtcgta gagtgtttgg aatcggcaga gactgttttt ttatatgggg
    12781 ttggcgcctc ttctttagta gtagaagata ttctgcaaaa atggtctcgt gtcggtaagc
    12841 caatcatttt tgaaaaagat atccacgtct tgctcccgca acttgtcagc aatgagaaaa
    12901 agaaagtgct ttggcttgtt tcaaactcag gtagaagtgc agatgtagtc gcattagccg
    12961 agctggcgaa aagcatgaat atcgaaatca ttgcgttgac gcaatttgga aataacccgc
    13021 ttagcaagat agccgatgta ctcgtacaaa catctagacc aaaagaaatc acgaatcgaa
    13081 gtgcggcaac taattcactt ttggcccaat ttgctacgat cgatatcatt ttttatttct
    13141 atatggctaa aaacgaaaag cttagcgaaa aagtgaaaaa aacacgagaa gcaatccaag
    13201 aatattttat ggattcgaaa taaaaacctt actatttttt atataagttt tttagaataa
    13261 caaaacgtag ttttccacaa ataaatgatc catcaaagtg tttcacgact taagaaaaca
    13321 taattttcaa aaataagttt attttttaat gaaaaaaata gttggatatg taactttaag
    13381 tgattgctgt gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa tttcttgctc
    13441 cgcccatttt ttctt
//
LOCUS       DAFBYI010000072        13333 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFBYI010000072 DAFBYI010000000
VERSION     DAFBYI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.072"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="LLD04_001880"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="LLD04_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK5648803.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="LLD04_001881"
     CDS             complement(740..1462)
                     /locus_tag="LLD04_001881"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK5648804.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="LLD04_001882"
     CDS             complement(1598..2479)
                     /locus_tag="LLD04_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK5648805.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LLD04_001883"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LLD04_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK5648806.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="LLD04_001884"
     CDS             3719..4198
                     /locus_tag="LLD04_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK5648807.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="LLD04_001885"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="LLD04_001885"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK5648808.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="LLD04_001886"
     CDS             5499..5627
                     /locus_tag="LLD04_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5648809.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="LLD04_001887"
     CDS             5631..6176
                     /locus_tag="LLD04_001887"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5648810.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="LLD04_001888"
     CDS             complement(6629..8320)
                     /locus_tag="LLD04_001888"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK5648811.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LLD04_001889"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LLD04_001889"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK5648812.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LLD04_001890"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LLD04_001890"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK5648813.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LLD04_001891"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LLD04_001891"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK5648814.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LLD04_001892"
     CDS             12601..13302
                     /locus_tag="LLD04_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5648815.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3750 a         2712 c         2366 g         4505 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aacgaaagtc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taa
//
LOCUS       DAFBYI010000073        13210 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFBYI010000073 DAFBYI010000000
VERSION     DAFBYI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.073"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            147..1016
                     /locus_tag="LLD04_001893"
     CDS             147..1016
                     /locus_tag="LLD04_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK5648816.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="LLD04_001894"
     CDS             1225..2250
                     /locus_tag="LLD04_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5648817.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="LLD04_001895"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="LLD04_001895"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK5648818.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5035
                     /locus_tag="LLD04_001896"
                     /pseudo
     CDS             3333..5035
                     /locus_tag="LLD04_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            5037..7070
                     /locus_tag="LLD04_001897"
     CDS             5037..7070
                     /locus_tag="LLD04_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5648819.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7081..7521
                     /locus_tag="LLD04_001898"
     CDS             7081..7521
                     /locus_tag="LLD04_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648820.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7721..8848)
                     /locus_tag="LLD04_001899"
     CDS             complement(7721..8848)
                     /locus_tag="LLD04_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648821.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8851..9528)
                     /locus_tag="LLD04_001900"
     CDS             complement(8851..9528)
                     /locus_tag="LLD04_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5648822.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9792..11558
                     /locus_tag="LLD04_001901"
     CDS             9792..11558
                     /locus_tag="LLD04_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK5648823.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11573..12010
                     /locus_tag="LLD04_001902"
     CDS             11573..12010
                     /locus_tag="LLD04_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5648824.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12023..13174
                     /locus_tag="LLD04_001903"
     CDS             12023..13174
                     /locus_tag="LLD04_001903"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK5648825.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4487 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaagaga tagcagatat
     4741 cacagaacaa aaaaagtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg
     4801 ctcacaaact atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt
     4861 tgttatctca aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc
     4921 acaaagagaa ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt
     4981 tgaacattgg gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt
     5041 atttatcgcc cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat
     5101 ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag
     5161 ataatcttga agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct
     5221 catttgtcat tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga
     5281 aaagtaatga cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga
     5341 cgacagagcc gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt
     5401 tttatgcaga tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc
     5461 ggaaccaggg atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg
     5521 tattagaatt agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac
     5581 tgaattattt ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg
     5641 gggaagaatt gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt
     5701 tgatgctgac attgacgatg gagcgtgtga ctaaaaatca tttactgaca aatgagacat
     5761 ataatagtct ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg
     5821 cttctgagac aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa
     5881 atgacttcac gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg
     5941 cttatttagt aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg
     6001 aagatgaaac tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg
     6061 ttttacaaga agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg
     6121 aagttgcgaa tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg
     6181 aagaagagat cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag
     6241 aaagcaatat cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg
     6301 aactacgatt gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg
     6361 atttaaaaaa aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac
     6421 caggatacgg tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc
     6481 agctaaaaga agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca
     6541 gcaataacga gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa
     6601 tcaatcaatt actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg
     6661 aaacgattcg cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg
     6721 ttcatcgccg gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata
     6781 tggggttatt ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt
     6841 tgaaacatcc gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt
     6901 tagttatgct ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca
     6961 gtggagcaat catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa
     7021 tcgtttatca gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag
     7081 atgatacagc taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa
     7141 gcgttgaccg agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc
     7201 caatcaatga tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg
     7261 ccacatggga ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg
     7321 cctgatggtg tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta
     7381 gtggctttaa aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca
     7441 gacatcgacc aagtaatggc gctcagtgat gcgaaaacca ttgaagaagt ccaaatgatt
     7501 tttgaacatg gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta
     7561 ataacttcaa ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg
     7621 cggatgcaag cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg
     7681 gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat
     7741 agatagtgcc catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac
     7801 cattcatgaa agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca
     7861 cattttgaaa atcaacataa gaagttggca agtaatgact gattgattct atccatccaa
     7921 tcgaagaaaa caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga
     7981 ctagagaatg ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc
     8041 acaacaacag gaacagaaag ctctggaaaa gatactcata catcggaagg attcgtatct
     8101 cctgaccaat gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta
     8161 ctagtataga gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa
     8221 tggtaaaacg gatgctatgt ttctttacca tactatccgg taatagattg actagagaga
     8281 ttacctgctt tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata
     8341 aaatcaaaaa actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaaggga
     8401 gatgcgctgg caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga
     8461 gcaactcgac acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca
     8521 gtcgatccag tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc
     8581 ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca
     8641 caaattcttt ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat
     8701 agttatgatt cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga
     8761 atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga
     8821 gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc
     8881 taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc
     8941 tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc
     9001 ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc
     9061 gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa
     9121 gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat
     9181 cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa
     9241 agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa
     9301 gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc
     9361 ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt
     9421 cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac
     9481 acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc
     9541 aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt
     9601 tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct
     9661 gataccgaaa tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc
     9721 aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag
     9781 ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac
     9841 aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct
     9901 ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag gctttggcta
     9961 ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa
    10021 aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg
    10081 tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg
    10141 ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag
    10201 tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga
    10261 ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat
    10321 ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga
    10381 ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg
    10441 gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg
    10501 cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc
    10561 actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat
    10621 tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg
    10681 gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca
    10741 attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt
    10801 ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc
    10861 aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta
    10921 cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga
    10981 tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca
    11041 atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac
    11101 tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt
    11161 tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag
    11221 tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca
    11281 aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc
    11341 tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag
    11401 atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac
    11461 agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat
    11521 acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag
    11581 tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg
    11641 ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat
    11701 ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg
    11761 aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga
    11821 tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc
    11881 aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat
    11941 ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc
    12001 aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg
    12061 aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa
    12121 tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga
    12181 atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga
    12241 aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat
    12301 cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag
    12361 cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga
    12421 aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc
    12481 aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca
    12541 agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca
    12601 attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag
    12661 tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat
    12721 tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac
    12781 aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga
    12841 aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg
    12901 gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca
    12961 agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta
    13021 tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga
    13081 acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca
    13141 aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg
    13201 acgaaatttt
//
LOCUS       DAFBYI010000074        13063 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFBYI010000074 DAFBYI010000000
VERSION     DAFBYI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.074"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(260..979)
                     /locus_tag="LLD04_001904"
     CDS             complement(260..979)
                     /locus_tag="LLD04_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5648826.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="LLD04_001905"
     CDS             complement(1028..2674)
                     /locus_tag="LLD04_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5648827.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="LLD04_001906"
     CDS             2853..3443
                     /locus_tag="LLD04_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5648828.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="LLD04_001907"
     CDS             3535..5040
                     /locus_tag="LLD04_001907"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK5648829.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="LLD04_001908"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="LLD04_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5648830.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="LLD04_001909"
     CDS             complement(6476..6994)
                     /locus_tag="LLD04_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648831.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="LLD04_001910"
     CDS             complement(7010..7336)
                     /locus_tag="LLD04_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK5648832.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="LLD04_001911"
     CDS             complement(7349..9328)
                     /locus_tag="LLD04_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5648833.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="LLD04_001912"
     CDS             complement(9349..9663)
                     /locus_tag="LLD04_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK5648834.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="LLD04_001913"
     CDS             9831..10124
                     /locus_tag="LLD04_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK5648835.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="LLD04_001914"
     CDS             complement(10202..11314)
                     /locus_tag="LLD04_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5648836.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11467..11691
                     /locus_tag="LLD04_001915"
     CDS             11467..11691
                     /locus_tag="LLD04_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648837.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="LLD04_001916"
     CDS             11701..11880
                     /locus_tag="LLD04_001916"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648838.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="LLD04_001917"
     CDS             12074..12244
                     /locus_tag="LLD04_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5648839.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>13063)
                     /locus_tag="LLD04_001918"
     CDS             complement(12668..>13063)
                     /locus_tag="LLD04_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5648840.1"
                     /translation="EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4109 a         2636 c         2223 g         4095 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatctat cgccagtttt ccatctgctc ctgtggttag acctgactgc agaacggttc
    13021 cattggcatc ttgtaactca aaaacagctc cttgcaggac ttc
//
LOCUS       DAFBYI010000075        13049 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFBYI010000075 DAFBYI010000000
VERSION     DAFBYI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.075"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="LLD04_001919"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="LLD04_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5648841.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="LLD04_001920"
     CDS             1873..4539
                     /locus_tag="LLD04_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5648842.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="LLD04_001921"
     CDS             4903..5655
                     /locus_tag="LLD04_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5648843.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="LLD04_001922"
     CDS             complement(5677..6831)
                     /locus_tag="LLD04_001922"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK5648844.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="LLD04_001923"
     CDS             7016..9457
                     /locus_tag="LLD04_001923"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK5648845.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="LLD04_001924"
     CDS             complement(9787..10491)
                     /locus_tag="LLD04_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648846.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="LLD04_001925"
     CDS             complement(10678..10923)
                     /locus_tag="LLD04_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648847.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="LLD04_001926"
     CDS             complement(10940..11791)
                     /locus_tag="LLD04_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5648848.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="LLD04_001927"
     CDS             11957..12961
                     /locus_tag="LLD04_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK5648849.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4169 a         2315 c         2716 g         3849 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DAFBYI010000076        13006 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFBYI010000076 DAFBYI010000000
VERSION     DAFBYI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.076"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="LLD04_001928"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="LLD04_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK5648850.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="LLD04_001929"
     CDS             complement(1258..3099)
                     /locus_tag="LLD04_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK5648851.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5596)
                     /locus_tag="LLD04_001930"
     CDS             complement(3281..5596)
                     /locus_tag="LLD04_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5648852.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="LLD04_001931"
     CDS             complement(5781..6641)
                     /locus_tag="LLD04_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK5648853.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="LLD04_001932"
     CDS             complement(6700..7221)
                     /locus_tag="LLD04_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK5648854.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7499..8260
                     /locus_tag="LLD04_001933"
     CDS             7499..8260
                     /locus_tag="LLD04_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5648855.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="LLD04_001934"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="LLD04_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5648856.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="LLD04_001935"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="LLD04_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5648857.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="LLD04_001936"
     CDS             12620..12976
                     /locus_tag="LLD04_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5648858.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3738 a         2718 c         2310 g         4240 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaa
//
LOCUS       DAFBYI010000077        12692 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFBYI010000077 DAFBYI010000000
VERSION     DAFBYI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.077"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LLD04_001937"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LLD04_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5648859.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="LLD04_001938"
     CDS             complement(321..890)
                     /locus_tag="LLD04_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK5648860.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="LLD04_001939"
     CDS             1076..1978
                     /locus_tag="LLD04_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5648861.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LLD04_001940"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LLD04_001940"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK5648862.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LLD04_001941"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LLD04_001941"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK5648863.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LLD04_001942"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LLD04_001942"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK5648864.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="LLD04_001943"
     CDS             8547..8732
                     /locus_tag="LLD04_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK5648865.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="LLD04_001944"
     CDS             complement(8794..9990)
                     /locus_tag="LLD04_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5648866.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="LLD04_001945"
     CDS             complement(10160..11596)
                     /locus_tag="LLD04_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5648867.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..>12692
                     /locus_tag="LLD04_001946"
     CDS             11889..>12692
                     /locus_tag="LLD04_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5648868.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLK"
BASE COUNT         3784 a         2668 c         2241 g         3999 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcactacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aa
//
LOCUS       DAFBYI010000078        12598 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFBYI010000078 DAFBYI010000000
VERSION     DAFBYI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.078"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(11..160)
                     /locus_tag="LLD04_001947"
     CDS             complement(11..160)
                     /locus_tag="LLD04_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648869.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            556..756
                     /locus_tag="LLD04_001948"
     CDS             556..756
                     /locus_tag="LLD04_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5648870.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="LLD04_001949"
     CDS             1324..1887
                     /locus_tag="LLD04_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648871.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="LLD04_001950"
     CDS             complement(1948..2292)
                     /locus_tag="LLD04_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648872.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="LLD04_001951"
     CDS             complement(2389..2634)
                     /locus_tag="LLD04_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648873.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="LLD04_001952"
     CDS             complement(2704..2958)
                     /locus_tag="LLD04_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648874.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="LLD04_001953"
     CDS             complement(3122..3445)
                     /locus_tag="LLD04_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648875.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="LLD04_001954"
     tRNA            3632..3705
                     /locus_tag="LLD04_001954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="LLD04_001955"
     CDS             complement(4090..4587)
                     /locus_tag="LLD04_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5648876.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="LLD04_001956"
     CDS             complement(4627..5436)
                     /locus_tag="LLD04_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5648877.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="LLD04_001957"
     CDS             5770..6627
                     /locus_tag="LLD04_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK5648878.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="LLD04_001958"
     CDS             6620..7522
                     /locus_tag="LLD04_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK5648879.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="LLD04_001959"
     CDS             7555..7905
                     /locus_tag="LLD04_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5648880.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7954..8877
                     /locus_tag="LLD04_001960"
     CDS             7954..8877
                     /locus_tag="LLD04_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5648881.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="LLD04_001961"
     CDS             8955..9848
                     /locus_tag="LLD04_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5648882.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="LLD04_001962"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="LLD04_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK5648883.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="LLD04_001963"
     CDS             10463..10651
                     /locus_tag="LLD04_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK5648884.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="LLD04_001964"
     CDS             10664..11566
                     /locus_tag="LLD04_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5648885.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="LLD04_001965"
     CDS             11598..11840
                     /locus_tag="LLD04_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5648886.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="LLD04_001966"
     CDS             complement(12096..12467)
                     /locus_tag="LLD04_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648887.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2366 g         3658 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DAFBYI010000079        12594 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFBYI010000079 DAFBYI010000000
VERSION     DAFBYI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.079"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(68..1099)
                     /gene="queA"
                     /locus_tag="LLD04_001967"
     CDS             complement(68..1099)
                     /gene="queA"
                     /locus_tag="LLD04_001967"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK5648888.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1292..2467
                     /locus_tag="LLD04_001968"
     CDS             1292..2467
                     /locus_tag="LLD04_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5648889.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2471..3112
                     /locus_tag="LLD04_001969"
     CDS             2471..3112
                     /locus_tag="LLD04_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648890.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3109..4026
                     /locus_tag="LLD04_001970"
     CDS             3109..4026
                     /locus_tag="LLD04_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5648891.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4023..4694
                     /locus_tag="LLD04_001971"
     CDS             4023..4694
                     /locus_tag="LLD04_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5648892.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4890..5724)
                     /locus_tag="LLD04_001972"
                     /pseudo
     CDS             complement(4890..5724)
                     /locus_tag="LLD04_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5925..6191)
                     /locus_tag="LLD04_001973"
     CDS             complement(5925..6191)
                     /locus_tag="LLD04_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK5648893.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6191..6454)
                     /locus_tag="LLD04_001974"
     CDS             complement(6191..6454)
                     /locus_tag="LLD04_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5648894.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6929..7114
                     /locus_tag="LLD04_001975"
     CDS             6929..7114
                     /locus_tag="LLD04_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5648895.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7256..9079)
                     /locus_tag="LLD04_001976"
     CDS             complement(7256..9079)
                     /locus_tag="LLD04_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK5648896.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9676..12381)
                     /locus_tag="LLD04_001977"
     CDS             complement(9676..12381)
                     /locus_tag="LLD04_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK5648897.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3623 a         2625 c         2375 g         3971 t
ORIGIN      
        1 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
       61 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
      121 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
      181 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
      241 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
      301 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
      361 catttttacg tacttggttc aatcgctcag ctgcttcttc cgtcaaacgg tagaattcac
      421 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
      481 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
      541 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
      601 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc
      661 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
      721 cgtgttccaa ttcttctttc actaccgctt gcaatctgcc atcaccaaaa gagattcttg
      781 tcccaacttt ggcacgtttc gcaggtttta tcaatgtctc ccacgtatct ccttgtgtat
      841 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
      901 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
      961 tttcatggaa atgtttatct tccatctctc caattttccg atccaaaaca agtaaacgag
     1021 aatgatcgcg gtcttttaat ggtgtttgtg cgatcaactc ttccggcaag tcaaaatcaa
     1081 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
     1141 tgtttttcat tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
     1201 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
     1261 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
     1321 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
     1381 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
     1441 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
     1501 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
     1561 catgacgatc cgtgaaaata tcacccttgt ccaaaaattg ttgaaagtca gtcaagaaga
     1621 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
     1681 tcgctaccca catgaattat ctggtgggca gcagcagcgt attggagtcg ttcgtgccct
     1741 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atcccatcac
     1801 tcgtgattct ttgcaagacc ttgtcaaaga tttacaagag cgcttgggaa aaacgatcgt
     1861 atttgtaacc catgacatgg atgaagcgat taagctagca agccgtatcg ccatcatgag
     1921 tgaaggccgt gtgatccaat ttgatacacc agaaaatatc ttacgtcatc ctgcaaacga
     1981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     2041 agtcggagaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     2101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     2161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aagcgaggaa aagtatccag
     2221 tgtcggcgac atattaaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     2281 taccttgcaa cgcattttaa aacgcggact gaaatacgtg ccggtcgttg atgaacagaa
     2341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     2401 gggagaagat cccgcaacta ctgatgttgc agagtcagat actcctgaag aatctaagga
     2461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     2521 tgaacatctt tatatttcgg gacttgcttt gatcattggt atcatcattg ctgtaccgct
     2581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     2641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     2701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     2761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     2821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     2881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     2941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     3001 tttggcaggt acgatccctg taacgatttt agcactattg gctgattggc ttctaggtct
     3061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     3121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc tttccctggt
     3181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     3241 cagatcttag caagcctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     3301 atcatcaaca atctggctac cacaactatc aaccatcaag cgatgatgaa cggagatgct
     3361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     3421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacgat
     3481 caaacgtggt tcccgtccta tggttttgaa aatacgtatg tctttcttgt tcgaaaagac
     3541 accgcacaaa aataccattt atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     3601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     3661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     3721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     3781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     3841 ccagttgtaa acaacaaaat attagctgat acgcctcatt taaaagaagc cttaaataag
     3901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     3961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
     4021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
     4081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
     4141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
     4201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
     4261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
     4321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
     4381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
     4441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
     4501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
     4561 gaaccaatgc aacagatggc acatcgatta ttttagcagg tgcattgcca actgcattaa
     4621 tggtgattgt cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
     4681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
     4741 aaggcttttt tagttctgtc attttttaag gcaataatcc actctgattc ttccctcctt
     4801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
     4861 gccaacgggt tcatctctgc atccaccgac taagtttttt tcttatccgc taaggcattt
     4921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
     4981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
     5041 agccttttcc atcagtcctc gattgttcga tgccttcagc tacatcatca aaactggtga
     5101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
     5161 tggctaaaaa tatatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
     5221 accggtacat gattcttatg gactgcatcc gtcgcaacta gacttggcgg tacgatgatc
     5281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
     5341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
     5401 cgaaagcgcc acgactttga cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
     5461 tttggatacc cgctctgcta aagaaacgac actggtattg tatttcgtat ctgtatcttt
     5521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
     5581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
     5641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
     5701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
     5761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
     5821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
     5881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
     5941 caggcagaat aaatttccac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
     6001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ctcaaaagac
     6061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
     6121 aaattcgatt gcttgatttt tcttaaatcc actgtcgcag agtttttgat tgcgactgta
     6181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ccactgtcgt
     6241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
     6301 gaatcgctct ccagtcttct agcccaatca atacagcatt gtttccacta cgatcactaa
     6361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
     6421 gagctgtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
     6481 ttataacgta cttgattaag tacgtcaaaa aatacaataa agcacttaaa ttcagcaaac
     6541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
     6601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
     6661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
     6721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacccacgaa
     6781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
     6841 tgattgcctc tttcgtctcc tgccattcat gttcactatc gatttgatac ttgcttagac
     6901 cgtcgggttt gtgaatcacc aattaaaaat ggcactgaat tatggtaaca aaaaatggtc
     6961 acttgacgag gctgagattt ctacttatga agacacatgg cacttaagag tcgaggtaaa
     7021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
     7081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
     7141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgggaataa gacaaaacag
     7201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
     7261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
     7321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
     7381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
     7441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
     7501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
     7561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
     7621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
     7681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
     7741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
     7801 accgatagac gaacaaaatc gcaatgattg catatgagat gaacgcacct gtaatattgg
     7861 caatcgactt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
     7921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
     7981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
     8041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
     8101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
     8161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
     8221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
     8281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
     8341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
     8401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
     8461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
     8521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
     8581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
     8641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
     8701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
     8761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
     8821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
     8881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
     8941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
     9001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
     9061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
     9121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
     9181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
     9241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
     9301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttgaaagag
     9361 agatgtaagg ttgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
     9421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
     9481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga acagcctttt
     9541 cacaacctct tggtacacgg tgttcaatca gctgaccctc ggcattagct caaaaaacgg
     9601 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacgctgatt
     9661 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     9721 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacag
     9781 accgatcatg atccagattg ctttcgtagc atttggccag taaatgcctc ctgcagattc
     9841 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     9901 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     9961 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    10021 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    10081 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    10141 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    10201 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    10261 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    10321 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcat ctagtttcaa
    10381 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    10441 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    10501 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    10561 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    10621 aaggatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    10681 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    10741 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgaact
    10801 gagaagcgaa tcaaagtcga tctggtccgc tttgcttagc gccccttgcg cagtcccaat
    10861 cgtatcgatg acttggtcta atagagaagt gacagtggaa ctgatttgac ctgtattgat
    10921 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    10981 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    11041 attgcttagc aagggtttta attgatttag ctgatcaata attggctgca atttcgtatt
    11101 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    11161 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    11221 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    11281 tgccacttgt ccaattgatt tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    11341 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    11401 taaatttttc acatctggca agatatcctg tacttgttgg atgatcgtca agccttgttt
    11461 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    11521 tgctagttgt ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    11581 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    11641 ctcatctact ttagggatat aatccacgaa ttcatttgct ttattcagtt tttccttgat
    11701 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    11761 attttcatct gttgataaaa tcatatcttt gactttattg atacttacta ggttcgtatc
    11821 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    11881 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    11941 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    12001 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    12061 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    12121 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    12181 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    12241 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    12301 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    12361 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    12421 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    12481 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagac
    12541 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatc
//
LOCUS       DAFBYI010000080        12346 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFBYI010000080 DAFBYI010000000
VERSION     DAFBYI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.080"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..514)
                     /locus_tag="LLD04_001978"
     CDS             complement(<1..514)
                     /locus_tag="LLD04_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HBK5648898.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFG"
     gene            complement(604..1149)
                     /gene="hpt"
                     /locus_tag="LLD04_001979"
     CDS             complement(604..1149)
                     /gene="hpt"
                     /locus_tag="LLD04_001979"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK5648899.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1149..2594)
                     /gene="tilS"
                     /locus_tag="LLD04_001980"
     CDS             complement(1149..2594)
                     /gene="tilS"
                     /locus_tag="LLD04_001980"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK5648900.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2714..3184)
                     /locus_tag="LLD04_001981"
     CDS             complement(2714..3184)
                     /locus_tag="LLD04_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK5648901.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3230..3655)
                     /locus_tag="LLD04_001982"
     CDS             complement(3230..3655)
                     /locus_tag="LLD04_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK5648902.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3722..3991)
                     /locus_tag="LLD04_001983"
     CDS             complement(3722..3991)
                     /locus_tag="LLD04_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK5648903.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(4002..5597)
                     /locus_tag="LLD04_001984"
     CDS             complement(4002..5597)
                     /locus_tag="LLD04_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5648904.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5612..9133)
                     /gene="mfd"
                     /locus_tag="LLD04_001985"
     CDS             complement(5612..9133)
                     /gene="mfd"
                     /locus_tag="LLD04_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK5648905.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9150..9710)
                     /locus_tag="LLD04_001986"
     CDS             complement(9150..9710)
                     /locus_tag="LLD04_001986"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK5648906.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            10063..11037
                     /locus_tag="LLD04_001987"
     CDS             10063..11037
                     /locus_tag="LLD04_001987"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5648907.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11207..>12346
                     /locus_tag="LLD04_001988"
     CDS             11207..>12346
                     /locus_tag="LLD04_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK5648908.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAG"
BASE COUNT         3477 a         2680 c         2227 g         3962 t
ORIGIN      
        1 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
       61 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
      121 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
      181 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
      241 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
      301 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
      361 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
      421 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
      481 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
      541 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
      601 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
      661 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
      721 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
      781 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
      841 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
      901 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
      961 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     1021 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     1081 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     1141 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     1201 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     1261 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     1321 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     1381 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     1441 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     1501 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     1561 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     1621 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     1681 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     1741 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     1801 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     1861 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     1921 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     1981 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     2041 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     2101 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     2161 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     2221 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     2281 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     2341 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     2401 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     2461 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     2521 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     2581 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     2641 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     2701 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     2761 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     2821 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     2881 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     2941 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     3001 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     3061 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     3121 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     3181 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     3241 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     3301 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     3361 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     3421 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     3481 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     3541 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     3601 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     3661 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     3721 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     3781 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     3841 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     3901 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     3961 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     4021 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     4081 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     4141 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     4201 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     4261 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     4321 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     4381 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     4441 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     4501 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     4561 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     4621 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
     4681 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
     4741 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
     4801 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
     4861 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
     4921 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
     4981 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
     5041 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
     5101 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
     5161 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
     5221 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
     5281 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
     5341 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
     5401 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
     5461 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
     5521 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
     5581 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
     5641 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
     5701 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
     5761 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
     5821 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
     5881 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
     5941 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
     6001 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
     6061 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
     6121 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
     6181 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
     6241 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
     6301 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
     6361 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
     6421 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
     6481 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
     6541 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
     6601 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
     6661 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
     6721 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
     6781 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
     6841 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
     6901 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
     6961 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
     7021 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
     7081 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
     7141 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
     7201 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
     7261 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
     7321 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
     7381 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
     7441 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
     7501 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
     7561 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
     7621 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
     7681 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
     7741 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
     7801 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
     7861 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
     7921 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
     7981 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
     8041 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
     8101 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
     8161 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
     8221 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
     8281 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
     8341 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
     8401 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
     8461 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
     8521 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
     8581 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
     8641 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
     8701 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
     8761 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
     8821 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
     8881 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
     8941 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
     9001 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
     9061 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
     9121 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
     9181 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
     9241 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
     9301 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
     9361 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
     9421 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
     9481 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
     9541 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
     9601 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
     9661 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
     9721 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
     9781 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
     9841 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
     9901 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
     9961 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    10021 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    10081 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    10141 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    10201 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    10261 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    10321 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    10381 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    10441 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    10501 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    10561 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    10621 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    10681 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    10741 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    10801 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    10861 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    10921 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    10981 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    11041 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    11101 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    11161 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    11221 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    11281 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    11341 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    11401 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    11461 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    11521 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    11581 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    11641 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    11701 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    11761 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    11821 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    11881 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    11941 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    12001 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    12061 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    12121 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    12181 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    12241 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    12301 aacagatatc gtttccggaa agccaactac acctacacct gctgga
//
LOCUS       DAFBYI010000081        12139 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFBYI010000081 DAFBYI010000000
VERSION     DAFBYI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.081"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(15..962)
                     /locus_tag="LLD04_001989"
     CDS             complement(15..962)
                     /locus_tag="LLD04_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK5648909.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1152..1421
                     /gene="rpsN"
                     /locus_tag="LLD04_001990"
     CDS             1152..1421
                     /gene="rpsN"
                     /locus_tag="LLD04_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5648910.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1597..1959)
                     /locus_tag="LLD04_001991"
     CDS             complement(1597..1959)
                     /locus_tag="LLD04_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5648911.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(1970..3091)
                     /locus_tag="LLD04_001992"
     CDS             complement(1970..3091)
                     /locus_tag="LLD04_001992"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK5648912.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3107..3457)
                     /locus_tag="LLD04_001993"
     CDS             complement(3107..3457)
                     /locus_tag="LLD04_001993"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK5648913.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3587..5098)
                     /locus_tag="LLD04_001994"
     CDS             complement(3587..5098)
                     /locus_tag="LLD04_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5648914.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5420..6772)
                     /locus_tag="LLD04_001995"
     CDS             complement(5420..6772)
                     /locus_tag="LLD04_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK5648915.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6925..8001)
                     /locus_tag="LLD04_001996"
     CDS             complement(6925..8001)
                     /locus_tag="LLD04_001996"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK5648916.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8197..9942
                     /locus_tag="LLD04_001997"
     CDS             8197..9942
                     /locus_tag="LLD04_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK5648917.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10191..10658)
                     /locus_tag="LLD04_001998"
     CDS             complement(10191..10658)
                     /locus_tag="LLD04_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK5648918.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10642..12015)
                     /locus_tag="LLD04_001999"
     CDS             complement(10642..12015)
                     /locus_tag="LLD04_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648919.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         3434 a         2613 c         2257 g         3835 t
ORIGIN      
        1 tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct
       61 tcaactagct gatgtgcttg gatgacatgc ttaagggaag cagacagatt cattgaaata
      121 aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct
      181 agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct
      241 agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg
      301 gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat
      361 tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc
      421 ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc
      481 tggatcagac ttgacccgac cgcacccgat gctcctagta tcattacagt atctgtcggc
      541 tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg
      601 gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg
      661 acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca
      721 gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa
      781 gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga
      841 ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga
      901 gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc
      961 atggtcacca tctccctttt tctttaagtt tacgtttatg cctttatttt gacaaaaaaa
     1021 atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt
     1081 gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga
     1141 ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga
     1201 ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc
     1261 gcaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgatttg atcgatggac
     1321 gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac
     1381 ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg
     1441 taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt
     1501 ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt
     1561 tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc
     1621 ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa
     1681 atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat
     1741 aacggagtct ctttctattc ctgttccagt agctttgatc ccaatatgag tcggaagctt
     1801 aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc
     1861 tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg
     1921 agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat
     1981 attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt
     2041 gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt
     2101 ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc
     2161 caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc
     2221 agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg
     2281 tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatt aattcagaaa
     2341 ccagctcaag tgctggctgc agtggataaa cttccggtag tgcgtgccca gagggattca
     2401 gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag
     2461 ccgttgcact attgctgaca tgtatatatc gaggaagttc gggaagtttt tttagcactt
     2521 ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa
     2581 aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct
     2641 cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat
     2701 gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct gcctgcaacc
     2761 attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt
     2821 aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc
     2881 cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg
     2941 tttcaatagc tccatgaccg tatccgtttg cttttactac tgcaaacaat tcttttcctt
     3001 gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg
     3061 ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa
     3121 taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccatact ttgccttcat
     3181 gaggtgaacg cgtcacaaca ggctgaccgt tttcattttt taaaatctcg acatcttgaa
     3241 aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac
     3301 gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat
     3361 cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag
     3421 gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat
     3481 ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa
     3541 caaaaatgaa agtttctctt aaaaaagaga aactttcatt ttatgtttaa tcgttatttg
     3601 aacggattac aaatccgcgt tttttatcgt tgtgacgttt accgccatct ttttggctgt
     3661 tattattttt gctataaccg ccttttgatt tgtttccacg ataattaccg ccgtcacgac
     3721 gattacggtt gtttccgcca ccacgtttgc catttttatt gtatcctttt ttctgcattg
     3781 gcaatgggcg ttctggtgtg atcttcactg gcactgcatc agctggatct tttgctgtcg
     3841 tttttagcaa aagtgcaact aaatcttgtg cagaatattc ttctaataat ttatctgctg
     3901 tttgcaggta cttatctaga ccattttctg caagttttgt ttcgatttgt tcaactgcag
     3961 cacctaattg acctttgaat gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt
     4021 ttttcgtcaa gttttcaatc acatgcaagt agtccatttc atttggtgta acgaaagtca
     4081 cagacatacc gccttttccg gcacggcctg tacgtccgat acggtgaacg tagctttctg
     4141 gatcttgagg gatatcatag ttgtacacat gagtgacacc tgaaatatct aatccacgtg
     4201 cagcaacgtc tgtcgctaca aggatatcta aatgaccgct cttaaatgaa cgcaatacgc
     4261 tcattcgttt ttgttgagac aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt
     4321 ctaatccgcg tgccaattcg tctacacggc gttttgtacg tccgaagacg attgttagtt
     4381 caggtgtttg aacatcaaac aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc
     4441 ggacatagta ttgatcgatc aagtcagctg tcatttcttt cgctttgatc ttcacatgat
     4501 gtggattttt catgaattta acgccaatat tcttgatagc tggcggcatc gttgctgaga
     4561 aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat tttttcgata tcttccaaga
     4621 agcccatatt caacatttca tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt
     4681 tcaatgtgtg gcggttgatg tggtcaagca tacgtcctgg tgtacccaca acgatatgtg
     4741 gacgatcttt caagccacgg atctgacggc caatatctgc tccgccgtaa acggcttgta
     4801 cacgtacttt tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg atagctaact
     4861 cacgtgttgg tgcgataact aaaccttgta attcgtgacg atctggatcg attttttcta
     4921 acattggtaa tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt
     4981 cttttccagc taatgctagt ggaatcgttt ctgattggat aggtgttgct tcttcaaaac
     5041 cagcgcgttc gactgatttc aacaattctg gtgataattc tagttcttta aatttcaaat
     5101 gaatcctcct atatggttgt taactgagga tgtagctttt ttgctacccc gtcgatttgt
     5161 tcggtcaaag tgtattgttt ctttttgaca gtggccacgc acgtttttat ccattcttag
     5221 cccctgtggc accaccgaaa aatcactttg cgaagtttag gcattagaaa agccaaacgg
     5281 gtcgcgttca gctttatcat tattatgaca tctggcttca acaagttgtt tctatggcaa
     5341 aaccagtcca atttctcttc atctttcggc aataggttat tctaacatag tttttcgttt
     5401 tctgcaagtt tacagatttt tatttcattt gctgaagctg ttctaccact tcaaataagc
     5461 ccatgctgtt gctgcctttt agcataatac tatctgttgc ttcaagattt tgttttacag
     5521 catcaatcaa tttattcttt tctttttcaa aatagaacac aggtagttgg gggtattttt
     5581 ggttcaattc ttcttttagt gcatgcattt gttccccata aaggaaaatc atggcgaatt
     5641 tctcaggatc gatatgttca ctcatttgcg catgcatatt taaagactct ttccctaatt
     5701 ccagcatatc tgctaacaca gcgattctgc gtccttttgt tggtagattt ttaaaagtat
     5761 ctaataccag tcccatggct gttggatttg cattatagac atcgctcaaa atagctgcac
     5821 catttcctgc cgttagccat tcggttctgt tttgagtgat agaaacatgg gcgagccctt
     5881 ggcgtatctc aggtatggac agtccaaaga agcgcccgat cgtatacgca atcaaagcat
     5941 tcgttacatt ataacttcca ggcagcggga tcgtaaatgt ttctccttct actacaaaat
     6001 cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac atcgccattt ccaataccga
     6061 atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg
     6121 gcacaacgag taatccgtct ggtacaagtc cgtcagtgat ttccattttg gctttagcga
     6181 ttccttctcg cgacccaaga ttttctatgt gtgcttcacc gatcattgtg attgctgcag
     6241 cttttggttg agccataagt gaaagttccg tgatttcatt ggcgtgatcc atgcccattt
     6301 ccaagacgat cttttctgta tcttggggca tgtgcaaaat cgtgtaagga agaccaatat
     6361 tattgttgta atttccttgc gtcttatatg ttttgtattt ttcagacaag acagacgcgg
     6421 ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact tctgcaccta
     6481 ctttttccaa gtaataggtt gccaattgct gcatggcttt caaaggatct gctacaggaa
     6541 taactggaaa atcttttgga gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc
     6601 cgtttgtgat cgcttgctgg ataaattcat gtccatctcg cgtaccagca agaggaacga
     6661 ataaacttcc ttcggttatc ttccggctgt caaattcgac attggtaatc tttgtcagtg
     6721 cagagcattc gctgcccacg gcttgtgcaa tttcttgtat tgttagattc attgagatct
     6781 ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc atgactctca
     6841 cttccaagta aatttctcta ttcgtattta gaagctagaa tgaacgcggt tacgcaatca
     6901 ctccagcttc ttctttattt ctctttattc atttttttca aaaaaagatt gacgctgatg
     6961 gtatcgattc attcctaatt gaatcagttc ttcaatcaaa tcaccgtatt tcaagcccat
     7021 attttcccat aagagtgggt acatactgaa ctccgtaaat cctggcatag agtttaattc
     7081 attcaggaat aattcgtttt tatttgtcaa aaagaaatcg caccggctca atccgcttcc
     7141 acctaacatc gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa cttcttccgg
     7201 cacttcggct ggaatctgca tttcgatttt attattgata tatttggctt cataatcata
     7261 gaatgctacg tcttttacga cttcgccagg caaagtcgtc cgaacatctt catttcctaa
     7321 tacagcaact tcgatttcgc gcgcttcaat tccttgttca acgattgctc gagaatcata
     7381 ctgataggct gttgctaaag cattttgcag ctcttctcgg ttttctgcct ttgtaatgcc
     7441 gacactagaa cccatattcg caggtttgac aaacatcgga taaagcaaag aaccttcaca
     7501 ttgatcaaat acttttttag gattttcttt ccattgattc ttaagtactg gtacataagg
     7561 aacttgcggc acaccagcag cttgtaaaat atacttggtc atgattttat ccatggcaca
     7621 tgcactggtc aatacgcctg cgccgacata aggcatattc aatgtctcta agaagccttg
     7681 gatcgttcca tcttccccgt ttggcccgtg taaaactgga aaaacgatgg ctccttcttc
     7741 tttgatttct cccggattga tcacttttcc tgtaaagcct tcctctgtct gtccacttgg
     7801 gtcccatgaa agatgcaaga catctttgct agcaggtttt tctgttagta atggaccttt
     7861 gacccattgt ccttcttttg taataaatac gagttgaact tggtaataat tataataaat
     7921 ggcatttaaa actgaaaatg cggaaagaat ggaaacttca tgctctgcgc tgcgtccgcc
     7981 atatagtaaa gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta
     8041 ctctcatatt ttagcacaaa acaaaaataa acgaataact tatttataaa aaaacgagtt
     8101 ggtttctaaa agaacaaacg tcatttattt tttgtcagct agattatttg tcccgttttt
     8161 tataaaaagt tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa tccacgtagc
     8221 cgcttggcag aaatcattcg agtgttcatc aattacaatg tagtgccaaa ctttgtacag
     8281 caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac taggccccac cttcattaag
     8341 ataggacaga tgctttctgt tcgagaagat ttgctttctt ctgccttcac tcaaacattc
     8401 aaaacattac aagatagtgt acctagcgac acattttcaa cggtcaaaaa gacgatcgaa
     8461 acagaacttt ctctttcatt atcagatata ttcgatgatt tttccaaatc gccttttgcc
     8521 tctgcttcta tggggcaggc tcatcgcgcc aagctgaaga acggtgactc ggtcgtcgtt
     8581 aagatccaac accctaacat cgccgaagag atccgacttg atcttcaact atttgaacga
     8641 gcgatccctt tgatcaaata cattcctgaa actagcgtag ttgatctaaa aggggtactc
     8701 caagaagtaa aacgttcctt gatcaatgaa atggacttct taaaagaaag tcaaaatggc
     8761 gaacaattct atcaaaagaa caatggctgg aaagagatcc gcagccccaa gatttatgac
     8821 gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa cttgaaccat
     8881 ttaatgaacg ccgaaaacaa aactgaaact tttatcactg gaattcaaaa caagcagctg
     8941 aaacaagaag ttgcaaagct tttagttgag aactttatga aacaggtatt tgatgatggt
     9001 tttttccatg ctgatcctca tccagggaat ctgctattcc atgtactaaa aaaagaagaa
     9061 cagacacaag ctagccgaaa aactgaaata gtccatgaaa aagagttcgg ttcctttgct
     9121 tttcgagcct ccacttctac agaagaccct gttgcgccat ataccatcaa ctacatcgat
     9181 tttggtatga tggggcatct ttctgcagga cttaggcaaa aactcacaca agccgtttta
     9241 gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa
     9301 gaaggctcgt ttgatgaaag tcggttccac caagaactga caagttttct tgaacagtat
     9361 tatgattctc ctattgatga aatcaacttg caagaagtat tcacacacgt cgtgacgatt
     9421 tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc ttttgctgaa agcatttggg
     9481 acattagaag gggtgatccg cgtccttgac cctgaagttt ccctcatgga agtcgcttcg
     9541 ccttttgccc aacattattt tctcactcat ctggatgtag aagatacatt gaaacaaagc
     9601 ggattagact tattggaagg gatgaaggct gcgcctaaga tccctcaaca gctccaccat
     9661 ctattagaaa tgtggacttc gggacaagga aaagtcaatt tggaattgaa aaagcaagac
     9721 aaattattga gcaggatcga aagtatgatc aatcggcttg ttttcggtat gattttagct
     9781 gcattgatcg tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc
     9841 gtttccttat tagggatatt tacgtatgct attgccgcat tcgtcattat ttttttggca
     9901 atcgatgcat tgatccaaat gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa
     9961 agaatcagct tcaagaaata agaagtgatt ttgcataaat tgtttctcaa atttttgtga
    10021 aaattcggct tatttttcga agaggctact tctgaagcac cgagaataaa aaatgtgaaa
    10081 aagctgttct gcacccagta ataagaacaa tcaaaaagga acatggagca catttatcct
    10141 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
    10201 tcttctgtac ttggtaaacc ttggtcaatt gcctttttta acgcttgaca aaaagacata
    10261 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
    10321 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
    10381 gagtgatgga acactcggac gcccttcggc aatacaagtg aagctaaacc ataatcttgc
    10441 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
    10501 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
    10561 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
    10621 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
    10681 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
    10741 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
    10801 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
    10861 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
    10921 agctgcatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
    10981 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
    11041 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
    11101 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
    11161 tttttctaat ccgtacatta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
    11221 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
    11281 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
    11341 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
    11401 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
    11461 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
    11521 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
    11581 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
    11641 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
    11701 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
    11761 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
    11821 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
    11881 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
    11941 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
    12001 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
    12061 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaataca
    12121 gaaagaagca taagcttct
//
LOCUS       DAFBYI010000082        12052 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFBYI010000082 DAFBYI010000000
VERSION     DAFBYI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.082"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            99..746
                     /locus_tag="LLD04_002000"
     CDS             99..746
                     /locus_tag="LLD04_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5648920.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LLD04_002001"
     CDS             1139..3784
                     /locus_tag="LLD04_002001"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK5648921.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LLD04_002002"
     CDS             4094..5407
                     /locus_tag="LLD04_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK5648922.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LLD04_002003"
     CDS             5397..6050
                     /locus_tag="LLD04_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5648923.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="LLD04_002004"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="LLD04_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK5648924.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="LLD04_002005"
     CDS             complement(6671..8023)
                     /locus_tag="LLD04_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5648925.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="LLD04_002006"
     CDS             complement(8089..9378)
                     /locus_tag="LLD04_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5648926.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="LLD04_002007"
     CDS             complement(9465..10169)
                     /locus_tag="LLD04_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK5648927.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="LLD04_002008"
     CDS             10369..10635
                     /locus_tag="LLD04_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648928.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            10648..10806
                     /locus_tag="LLD04_002009"
     CDS             10648..10806
                     /locus_tag="LLD04_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648929.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(10803..11033)
                     /locus_tag="LLD04_002010"
     CDS             complement(10803..11033)
                     /locus_tag="LLD04_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5648930.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="LLD04_002011"
     CDS             11105..11260
                     /locus_tag="LLD04_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648931.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            11417..11572
                     /locus_tag="LLD04_002012"
     CDS             11417..11572
                     /locus_tag="LLD04_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648932.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(11569..11793)
                     /locus_tag="LLD04_002013"
     CDS             complement(11569..11793)
                     /locus_tag="LLD04_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5648933.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
BASE COUNT         3858 a         2046 c         2275 g         3873 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 tagggggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga tt
//
LOCUS       DAFBYI010000083        11886 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFBYI010000083 DAFBYI010000000
VERSION     DAFBYI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.083"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..241
                     /locus_tag="LLD04_002014"
     CDS             <1..241
                     /locus_tag="LLD04_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5648934.1"
                     /translation="RIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLW
                     KGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
     gene            complement(287..1021)
                     /locus_tag="LLD04_002015"
     CDS             complement(287..1021)
                     /locus_tag="LLD04_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5648935.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1347..1757
                     /locus_tag="LLD04_002016"
     CDS             1347..1757
                     /locus_tag="LLD04_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK5648936.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1750..2451
                     /locus_tag="LLD04_002017"
     CDS             1750..2451
                     /locus_tag="LLD04_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK5648937.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2451..4064
                     /locus_tag="LLD04_002018"
     CDS             2451..4064
                     /locus_tag="LLD04_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5648938.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            4054..4275
                     /locus_tag="LLD04_002019"
     CDS             4054..4275
                     /locus_tag="LLD04_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK5648939.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4272..5033
                     /locus_tag="LLD04_002020"
     CDS             4272..5033
                     /locus_tag="LLD04_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK5648940.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5414..5923
                     /locus_tag="LLD04_002021"
     CDS             5414..5923
                     /locus_tag="LLD04_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5648941.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5993..6532)
                     /locus_tag="LLD04_002022"
     CDS             complement(5993..6532)
                     /locus_tag="LLD04_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK5648942.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6672..7283)
                     /gene="rpsD"
                     /locus_tag="LLD04_002023"
     CDS             complement(6672..7283)
                     /gene="rpsD"
                     /locus_tag="LLD04_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK5648943.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7545..8396
                     /locus_tag="LLD04_002024"
     CDS             7545..8396
                     /locus_tag="LLD04_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK5648944.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8424..9059
                     /locus_tag="LLD04_002025"
     CDS             8424..9059
                     /locus_tag="LLD04_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK5648945.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            9079..9852
                     /locus_tag="LLD04_002026"
     CDS             9079..9852
                     /locus_tag="LLD04_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5648946.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10260..11057
                     /locus_tag="LLD04_002027"
     CDS             10260..11057
                     /locus_tag="LLD04_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK5648947.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            11035..11448
                     /locus_tag="LLD04_002028"
     CDS             11035..11448
                     /locus_tag="LLD04_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5648948.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11432..>11886
                     /locus_tag="LLD04_002029"
     CDS             11432..>11886
                     /locus_tag="LLD04_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5648949.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGE"
BASE COUNT         3738 a         2097 c         2551 g         3500 t
ORIGIN      
        1 acgaatattt gcggatgcaa aagaaaagca tgggatgcgc tggaccaagt atcgaggctt
       61 ggaaaaagtc gcgacgcata cgatgctagt gtttgctgca atgaatttaa agaaattagc
      121 cacctggcta tggaaaggca aggagccttt atttttttgt tcaaaaattc gaaacgaagt
      181 agataaaaag ctgttccaag ctcgagttac gagtttggaa cagcttttgt ctacagtctg
      241 acttggtacg caatcgctgt accaagtttt tttatttagc ttttgattaa gccataaata
      301 ataatttata taagaatact gctacgacac cagcacagat aggtgctaca actggtaccc
      361 atgcatatcc ccattgagaa gaacctttat gtttcaatgg aagaagtgcg tgtacaaaac
      421 gtggagcaaa gtcgcgggct gggtttagtc caggacctgt aggaccacca agtgaagcaa
      481 ctaatgtcca tacaaggaaa ccgagtgcta agtgagctgt accaagatta tcttggaaga
      541 atggtgcttt tgtaatacct aaggcaccaa agaataaaac gaatgaacca aagaattcat
      601 tgacgaaacc attgaattta gaaccaacgc tgttgattgt tgagaatgta cctaacacat
      661 ggttcacgtc ttctgtttgg tcgtagtaag gtttgtaaca agctactacg atcaattgtc
      721 caatcgctgc accaatcaat tgtgcaatga tataaggaac aacttcagcc caagggaata
      781 gaccattaga tgctaaagca attgtaaatg ctgggttgat gtggttaccg ctgattccac
      841 caaaaatcat tgctggcatc ataaccccga atccataccc gacagcaatc agcatccagt
      901 cactgccata tccttttgta ccttttaaat ctacgtttgc tacagcaccg ttacctagaa
      961 cgattagtaa tgctgtacca atcatttccg ccgcaagacg ggtcattagt gagtaatcca
     1021 taaatacctc caaaagttat aaaaatgcaa aaaaaaagaa acatcaacac gaaaagcatc
     1081 ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa taaaaaaatg
     1141 aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt ttaaaagttc
     1201 tccacaaacc accaagctta actataaata taccaaaaaa aaaacgtttt ttttctcaaa
     1261 aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt agtaaaatga
     1321 aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc attattattt
     1381 tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt cctggaagta
     1441 tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat cctgaaaaaa
     1501 tcggagcaac tggtgatttt ttattgaata atctagctat tttatttgtt cctgctggtg
     1561 taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta ctgggagcag
     1621 tcgtcgtgtg ttccatcgtc acgatggttg cagagggaaa aacggcagaa ggtgtagaag
     1681 ctttgctggg ctatgtccgt aagaaaaaag caggaagggt atctgtaaac gaaatggagg
     1741 aaaatcaaat tgactgagtt ttttgtgaat ccactatttg gcttaagttt gactttagga
     1801 gtgtatgtat tatctgataa atttttgcgg aaatataaac tgcctgtttt gaatcctctc
     1861 gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag ctataaagac
     1921 tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt ggcacttgcc
     1981 atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt aatggctgcg
     2041 attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta cttcttctct
     2101 cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc tatttctgtc
     2161 gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt tgtctctaca
     2221 ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat cgaaagtcca
     2281 gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta tcggtacagc aaaagcaatc
     2341 gagattggcg aaatagaagg aatcatttcc ggacttgcga tatgcgtaaa tgggatattg
     2401 accgttttgt tgctgccgtt attctttcag ccttttgctg gcttgttttg atgcgaaaac
     2461 cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg aaagacgtgt
     2521 atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag attctcctcc
     2581 tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa gttgttcagc
     2641 tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat cagcagttta
     2701 gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac ttttatgata
     2761 cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt acgctgaaat
     2821 tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa tttgcgcaaa
     2881 agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa cagcaactgc
     2941 aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa gaagaaatca
     3001 ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc tggccatttg
     3061 aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct ttttttaatg
     3121 ttcgtctgaa cgagatcaaa aaaagacgat tggaatacat gctaggtgcc ttttttttaa
     3181 gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg atttctgaca
     3241 atctgttgtt ccagcgattt tgccaagtca tggagccggt atttcctaat tattttcaag
     3301 tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa tattatagtg
     3361 atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca ccgcccttta
     3421 cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag gggctgccag
     3481 cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt ttcgcctatc
     3541 tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt aatcatttag
     3601 ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt agacacaaac
     3661 atcccaatca tctggcattt aaaaatcatg cgtttttgat gggtcggtat ctgactgtgt
     3721 ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt ttaatgacag
     3781 atttcccatt atttgaagag caactgttag aggagggatt acggacattt ttccgtaatg
     3841 agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat cttctgattt
     3901 cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt gtgaccgaag
     3961 aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt caaaaaaaaa
     4021 ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat tttaccagaa
     4081 ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact ttatgttgtt
     4141 ctcgtatccg atgatgaatt tgatggctgt ccagtgacac cttacttatt gatcaaaaca
     4201 agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc agatgattgg
     4261 gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta tttatcacag
     4321 gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa gatgcttttg
     4381 tatttggatt agatcaaaag ttcggtaaaa tggaagaact ctctttgcga tttcctgaaa
     4441 cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa gcggtatcga
     4501 aatgccagga tcgttttggt gaagttacga ttttactgaa tacatcgggg atattggatg
     4561 cctacaaacc attgatggaa actgacgaat cgctgtggga tcttatttat gagacgaatg
     4621 tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc cgaaaaagcg
     4681 gaacaatcat caacatggct tcgattgcgg gtttggttgc tggcggaggt gggatcgctt
     4741 atacaagtgc gaaacatgca atcgtcggat ttaccaaaca actcgctctg gattacgcag
     4801 gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca atgaatgcgg
     4861 ctgattttga agggaaaggt gagatggctg agtgggtcgc aaatgaaaca ccagtcaagc
     4921 gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct cctcaagcaa
     4981 gctacataca aggaacgatc atcccaatcg atggcggatg gttgttaaaa taaccttgtg
     5041 tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga tgataaaaga
     5101 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     5161 taatcgttaa tattcgaaac taagcggctt ttcacgacct tttgatataa acggtgttca
     5221 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5281 ttgactattc ttattacttg ttgcagggca gctttttcac taccttctgc ggcacttcag
     5341 ttggaatttt cttcccatcc tttggtcgca gtcattacca agataaaatt atcttgcaaa
     5401 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5461 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5521 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5581 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata tggcttctcc
     5641 tcttggactg atagatgttg tagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5701 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggctgtga cagcatgcgt
     5761 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5821 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5881 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5941 aatatggcag cgtggaagag actttgagtc ttcttccatg ctgctttttt gattaatttg
     6001 tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct gctccagttg
     6061 ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca gttgtaaaag
     6121 taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca agagagccca
     6181 ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc atccaaccag
     6241 ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca aataaaacag
     6301 ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt aaaagataaa
     6361 ctacaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat ccaatagcaa
     6421 atgtttgccc cgtcaaagga accaagccaa aaggaatggt catctgagaa aggacagaaa
     6481 taatcgcggc aaattccgca gcaagtatta attctttcaa tgttcggttc ataatgccct
     6541 cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa gtgattactt
     6601 tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct ttttcaagaa
     6661 agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc gtcgatttct
     6721 ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt ttcagcgtca
     6781 aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac tgttacgatg
     6841 ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta tgaagggata
     6901 tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg tgcttgacga
     6961 cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc tagtaagatc
     7021 atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa caagttacgg
     7081 aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt taattgcatg
     7141 ccgtattctg attgtttgcc acggctgtta ggtccatgtt gacctggtgc gtaagggcga
     7201 cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga aattttccat
     7261 gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt tttggagtaa
     7321 aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca cctttgcagc
     7381 cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt attatttttc
     7441 tgctgcatta ttttacacaa actacagtat atgatagatc atccacgaat gtcaagataa
     7501 ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga gggcatgtgc
     7561 aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta tcaccattat
     7621 ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac agcttttcac
     7681 gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg gctgtggcat
     7741 ttgcaatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt ttatacgcat
     7801 ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgctgaatta ggaacatcga
     7861 atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt aaggcagcca
     7921 agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt ggatttctta
     7981 tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta gaatcaatag
     8041 cagtaaaact agcaaaacct gcagcaacga ttttggtgga agggatgttt gcgaatatcg
     8101 ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgccggg aaagtgatca
     8161 cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg atagcgaatt
     8221 ttcctgcctt tacgcttgct tactttgctt ctcaaggaca gacggatgga atgacagtca
     8281 gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt ggcggtttag
     8341 taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg gactaaaaag
     8401 tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat gaggaaaaag
     8461 cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta cctaagccgg
     8521 aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct atttctgaag
     8581 ttttagcttt tgtttattat tattatgaac aaatcgaaag agagtatcag tatgtagcga
     8641 tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc gtattgttgc
     8701 cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa gtatacagca
     8761 catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt gaaaaaaagg
     8821 aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa cagcaaacag
     8881 acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa ttctttatcc
     8941 aaaaagcaat tggttgggca ttacgcgatt acagtaagac cgatgccgat tgggtaaaaa
     9001 gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag tatttataag
     9061 aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga tggaacgttg
     9121 ttagattcaa acaaacaagc tttagatagc acgagagaag cattggaaaa attacgcaaa
     9181 gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca agagatcatt
     9241 cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt tttagaccat
     9301 gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt tcaagaagcg
     9361 catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg ttcttcttcc
     9421 aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt gcctgaatta
     9481 gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt cgatcaaaca
     9541 tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca tgagaacagt
     9601 gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt agcagaacaa
     9661 gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga ccgtgaaatg
     9721 ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt acaagcagaa
     9781 gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt gaaagagtta
     9841 agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gcccagccaa
     9901 acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga tgcaagcttt
     9961 tgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa ataagaaggg
    10021 atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg aagaagtaga
    10081 tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata gtcccatttt
    10141 tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt ttgacctttt
    10201 ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag gaatttagtg
    10261 tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg attactgaat
    10321 atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat ggtgagaatg
    10381 ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa gacggcgtag
    10441 atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag tttgtaaatg
    10501 aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga caagggatgg
    10561 tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct cgttctttta
    10621 tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa gccagaaaac
    10681 gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa caagcaatgg
    10741 gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac gttcagacaa
    10801 atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg atgacaggtc
    10861 cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt ctgaccgcat
    10921 taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc tgtatcttgg
    10981 aattggatga tacgacaggt catgcaaaaa aaatccagct gattcagatc aacgatgatc
    11041 gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga aattcaaaaa
    11101 gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg ctacaaagaa
    11161 cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa aatcaaacaa
    11221 gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga acaagaagca
    11281 atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt tgtagcttat
    11341 agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat gatacaagaa
    11401 gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa atactcctat
    11461 gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt tctatcgttt
    11521 aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc ttttggaatt
    11581 aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg gtgtacctca
    11641 ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag tagcgatttg
    11701 cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag tgatccagct
    11761 agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca acaatttttt
    11821 aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt taagtacggg
    11881 agaatt
//
LOCUS       DAFBYI010000084        11613 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFBYI010000084 DAFBYI010000000
VERSION     DAFBYI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.084"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            127..3654
                     /locus_tag="LLD04_002030"
     CDS             127..3654
                     /locus_tag="LLD04_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK5648950.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LLD04_002031"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LLD04_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK5648951.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LLD04_002032"
     CDS             7442..7783
                     /locus_tag="LLD04_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648952.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LLD04_002033"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LLD04_002033"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK5648953.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LLD04_002034"
     CDS             9140..11560
                     /locus_tag="LLD04_002034"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK5648954.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
BASE COUNT         4006 a         1985 c         2498 g         3124 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttg
//
LOCUS       DAFBYI010000085        11490 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFBYI010000085 DAFBYI010000000
VERSION     DAFBYI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.085"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            133..2604
                     /locus_tag="LLD04_002035"
     CDS             133..2604
                     /locus_tag="LLD04_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5648955.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="LLD04_002036"
     CDS             complement(2707..4443)
                     /locus_tag="LLD04_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK5648956.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="LLD04_002037"
     CDS             complement(4440..5144)
                     /locus_tag="LLD04_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5648957.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="LLD04_002038"
     CDS             5275..5778
                     /locus_tag="LLD04_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK5648958.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="LLD04_002039"
     CDS             5738..6076
                     /locus_tag="LLD04_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK5648959.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="LLD04_002040"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="LLD04_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK5648960.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="LLD04_002041"
     CDS             7627..8205
                     /locus_tag="LLD04_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK5648961.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="LLD04_002042"
     CDS             8209..8730
                     /locus_tag="LLD04_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5648962.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="LLD04_002043"
     CDS             complement(8809..9363)
                     /locus_tag="LLD04_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5648963.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="LLD04_002044"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="LLD04_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK5648964.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="LLD04_002045"
     CDS             9903..10154
                     /locus_tag="LLD04_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK5648965.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="LLD04_002046"
     CDS             10279..11070
                     /locus_tag="LLD04_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648966.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..>11490
                     /locus_tag="LLD04_002047"
     CDS             11240..>11490
                     /locus_tag="LLD04_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK5648967.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGI"
BASE COUNT         3793 a         2116 c         2365 g         3216 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt
//
LOCUS       DAFBYI010000086        11339 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFBYI010000086 DAFBYI010000000
VERSION     DAFBYI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.086"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..936
                     /locus_tag="LLD04_002048"
     CDS             <1..936
                     /locus_tag="LLD04_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5648968.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="LLD04_002049"
     CDS             939..3122
                     /locus_tag="LLD04_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5648969.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="LLD04_002050"
     CDS             3119..4120
                     /locus_tag="LLD04_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5648970.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="LLD04_002051"
     CDS             4135..5049
                     /locus_tag="LLD04_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5648971.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="LLD04_002052"
     CDS             5294..5410
                     /locus_tag="LLD04_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5648972.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="LLD04_002053"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="LLD04_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5648973.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="LLD04_002054"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="LLD04_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK5648974.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10641
                     /locus_tag="LLD04_002055"
                     /pseudo
     CDS             9380..10641
                     /locus_tag="LLD04_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            10886..>11339
                     /locus_tag="LLD04_002056"
     CDS             10886..>11339
                     /locus_tag="LLD04_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBK5648975.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1896 c         2374 g         3334 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tgttttttag agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac
    10381 aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc
    10441 actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag
    10501 gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa
    10561 cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga
    10621 gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg
    10681 ttttgaactt gttctttctt atcttgatat atatagaaat aacgtcattt ttattttagt
    10741 tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa
    10801 aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac aaataactaa
    10861 atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa
    10921 atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa
    10981 aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag
    11041 agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa
    11101 gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc
    11161 aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac
    11221 agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa
    11281 taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaatta
//
LOCUS       DAFBYI010000087        11253 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFBYI010000087 DAFBYI010000000
VERSION     DAFBYI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.087"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(40..1419)
                     /locus_tag="LLD04_002057"
     CDS             complement(40..1419)
                     /locus_tag="LLD04_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HBK5648976.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="LLD04_002058"
     CDS             complement(1440..2987)
                     /locus_tag="LLD04_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5648977.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="LLD04_002059"
     CDS             complement(3236..4015)
                     /locus_tag="LLD04_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5648978.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="LLD04_002060"
     CDS             complement(4090..4548)
                     /locus_tag="LLD04_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5648979.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5834)
                     /locus_tag="LLD04_002061"
     CDS             complement(4560..5834)
                     /locus_tag="LLD04_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBK5648980.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="LLD04_002062"
     CDS             complement(5935..6249)
                     /locus_tag="LLD04_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5648981.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="LLD04_002063"
     CDS             complement(6395..7891)
                     /locus_tag="LLD04_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBK5648982.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="LLD04_002064"
     CDS             complement(8069..8548)
                     /locus_tag="LLD04_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBK5648983.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            8715..8864
                     /locus_tag="LLD04_002065"
     CDS             8715..8864
                     /locus_tag="LLD04_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648984.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(8924..9169)
                     /locus_tag="LLD04_002066"
     CDS             complement(8924..9169)
                     /locus_tag="LLD04_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5648985.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="LLD04_002067"
     CDS             9274..10047
                     /locus_tag="LLD04_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648986.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="LLD04_002068"
     CDS             complement(10276..10524)
                     /locus_tag="LLD04_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5648987.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="LLD04_002069"
     CDS             complement(10623..10937)
                     /locus_tag="LLD04_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648988.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11074..11220)
                     /locus_tag="LLD04_002070"
     CDS             complement(11074..11220)
                     /locus_tag="LLD04_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5648989.1"
                     /translation="MILNNPEYIESGKAETVNYYYELPSEIQNYQIVIREADGYFVGL
                     PFTL"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DAFBYI010000088        10996 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFBYI010000088 DAFBYI010000000
VERSION     DAFBYI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.088"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            128..1387
                     /gene="lysA"
                     /locus_tag="LLD04_002071"
     CDS             128..1387
                     /gene="lysA"
                     /locus_tag="LLD04_002071"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK5648990.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1374..2174
                     /gene="dapF"
                     /locus_tag="LLD04_002072"
     CDS             1374..2174
                     /gene="dapF"
                     /locus_tag="LLD04_002072"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK5648991.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2177..3058
                     /locus_tag="LLD04_002073"
     CDS             2177..3058
                     /locus_tag="LLD04_002073"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK5648992.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3055..3771
                     /locus_tag="LLD04_002074"
     CDS             3055..3771
                     /locus_tag="LLD04_002074"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK5648993.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3777..4472
                     /gene="dapD"
                     /locus_tag="LLD04_002075"
     CDS             3777..4472
                     /gene="dapD"
                     /locus_tag="LLD04_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK5648994.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4488..5489
                     /locus_tag="LLD04_002076"
     CDS             4488..5489
                     /locus_tag="LLD04_002076"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK5648995.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5486..6847
                     /locus_tag="LLD04_002077"
     CDS             5486..6847
                     /locus_tag="LLD04_002077"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK5648996.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6807..7958
                     /locus_tag="LLD04_002078"
     CDS             6807..7958
                     /locus_tag="LLD04_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK5648997.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7933..9102
                     /locus_tag="LLD04_002079"
     CDS             7933..9102
                     /locus_tag="LLD04_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5648998.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9277..10656
                     /locus_tag="LLD04_002080"
     CDS             9277..10656
                     /locus_tag="LLD04_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5648999.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3660 a         1917 c         2459 g         2960 t
ORIGIN      
        1 aaaaaatatt tcgaaatata ctagacaatt cagaaaattt tgtttaatat tattccaata
       61 aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg aaaaaaagtg aaaagaggaa
      121 tgaaaaaatg acagaattga taattggcgg agtcacagca agcacattgg cagaaaagta
      181 taagacacct ctgtatgtgt atgatgaaga aaagatgaga caaacgatgc tgtcattcaa
      241 gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt tacgcctcta aagcattcca
      301 aacgattgag atgctgcatt tagttcgtga atatggattc gggctagatg ttgtcagtgg
      361 tggggaaatc tataccgctt tgcaagctga ttttcctaga gatactatct acttccatgg
      421 taacaacaag acgccagagg aactgatcta tgcacttgaa aatgatttgt tgcatattgt
      481 tgccgataat ttgatggaag ttgaattatt agcagaatta agccaacgct atttaaaaaa
      541 aatttgtatc atgcttcggt tgaatgtcgg agtagaagca catactcatg aatatatcgt
      601 cactgcgcat attgattcaa aattcggcat gagctatgaa agtaaagagt gccaacaagc
      661 ccttcagttg ataaaagaaa gcagctatct agagttagaa ggatttcatg ctcatatcgg
      721 gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc gacaaattga cgaattactt
      781 aaccgatttt gaccaacctc tttccttgaa tcttggcgga gggtttggga ttcgttatac
      841 acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa aaactgattg aatatacgga
      901 agaagctttg gaaaaacgaa aactgaccat tcgtcaatta ctgatcgaac cgggtagaag
      961 tattgttgga gaagctggta caacactata cacgattggt ttcatcaaaa agacaccaca
     1021 taaaaaatat tactttgtag atggcgggat gacagataat atccgtccag ctctgtatca
     1081 ggcagaatac gattgtgatt tagcagcaaa actgtcggaa gaaaaaacag agaaagtgac
     1141 gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt aaagaagctt atttgccgaa
     1201 agcagaatcg ggtgatcttt tagctgttta ttccacggga gcttatggat actcgatgag
     1261 cagcaactac aatcgagcga cacgaccagc agttgttttt gtcaataatg gccaatcaag
     1321 actgattgtt cgcagacaag cattttctga tctactaagg ggggaagttt cctatgatcc
     1381 ttcataaata taatggctgc ggaaatgatt ttatcttatt agattacgca gaagacacag
     1441 attatagtca attagccgct tctttatgca caaaagaaaa atatgatata gacggattga
     1501 tcgccgtaaa aatcgatccg ttggaaatga tctattacaa caaagatggc agccgcgcgc
     1561 cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt aaatgatcag aacatgatga
     1621 atgaacagga attcaacgta gagacattag caggcacaag aaaaattacg gtgttggatc
     1681 cctcaccttt ttattgcagc gtagatatgg gagtgccaga tgattctccg gaaaaaatca
     1741 aagctaatca aacttatcca ataaaaaacc aggaaataga gatcaatggt gaaaaagtaa
     1801 cgatcactag tttattttta ggaacgattc ataccgttgt ttttgttgaa aatgcgcgtg
     1861 aggaagtctg gagaaaaaga ggtgaactga tctgtcatca tcctttgttc aaagaaaaaa
     1921 ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt gatcgtccgt acctatgaaa
     1981 gaggagtagg ttggacactt gcttgcggta caggttgctg tgcctcttat gtggttgcta
     2041 aggaaactgg gaaaatttca gcagatcagg tagttgttca cttggaacaa ggagacttga
     2101 tgatctctgg tgaggatgcg atacaaatgg ctggcccagc agttcatgaa tggacaagaa
     2161 tattggagga atgaagatga taaaaggttc gatcgtggcg ctgatcacgc cgatgaatga
     2221 agagggatca gtagattatg ctggtttgga aaaattgatc caattccatt tagatgaaca
     2281 aacagatgga ttattagttt taggtacaac aggagaatct tctacgctca cacaatctga
     2341 ggaggagcag atccttcaac tgacagtaaa gaaagtggct ggccgtgtgc cggtgattgc
     2401 aggagctgga acgaataata cgaaagaaac gattgaaaaa gcgaagcatt ttgcttcttt
     2461 gggggcagac gcacttttgg tcatcacccc ttactacaac aaaacaagtg atgcaggact
     2521 agctgctcat tttacagcaa ttgcagaagc ctcaccgatt ccgcttattt tgtataatgt
     2581 tccttctaga acaggcatgt cgattccaat ccatgtatta gtaaatttag cagaacatcc
     2641 taatatcatt ggtttgaaag aagcctcagg agatatggct tatgtgatgg atgccgctcg
     2701 gttgattgga gaagaattct tcctttacag tggaaatgat gatttgatcc taccagtaat
     2761 gagtgtagga ggaagcggtg taataagtgt atgggcgaat atccaaccta aaatagtcca
     2821 tgaattagta aaagatacgc aggacggaag atggcagcaa gcaaaagaaa agcaattaaa
     2881 tgcacttgaa ctgatccatg ccttgttcag tgagaccaat cctattcctg tcaaagcagc
     2941 catgtcatta ttagacttac cttccggacc tctacgcctg cctttagttt ctctttcaaa
     3001 agagaaaaag aaacaattag ctcagctttt gttaaaagaa aggacggttg aaaaatgaaa
     3061 atcggactga ttggcagtgg acagatggga aatagagtcg aagaagttgc acaagaaaga
     3121 ggagatacgg tccttctttt acggacggca cctaaagaag caactacgct tgcttttacg
     3181 gaactgccag aactgcttat tgatttttct catccagata atttagagaa aatcctttcg
     3241 tatagcttat catatcaact tccgttagtc attggcacga caggttatac acaatcacag
     3301 ttccaagaaa taaaagaaca tgccaaacat gtacctgtga tgtatagtgc taatttttcg
     3361 ttcggcatca tggtgatgaa tcaactaatc aagcaagcag ctgcaatgtt gcaaggctgg
     3421 cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag atgcaccatc tggtactgca
     3481 aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc agcccgtata caactggcaa
     3541 gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta ttcgtgcagg ttctttacct
     3601 ggtgaacatg atgtattgtt tgctgctact gatgaaatat ttacgatcaa acatgaatct
     3661 ttttctaatc gaatctttgc tgcaggcgca gtagaagcag cggactggtt gaaagatcag
     3721 tcaccaggtt attacaaatt agaagattta ttaatggaca aaggggtgta aaaaacgtgg
     3781 aagctcaaga aatcatccgc tacattcaaa cggcatctaa aaagacgcct gtgaaagtct
     3841 atctgaatgt aaccgagcca atcgaattta aagaaagtaa agtttttggt gaaggaagca
     3901 gcttcgttgt ttttggtgac tatgagacga ttgctccggt tttagaagct gaatcagaaa
     3961 aaatcattga aatcgaaatc gaaactgctg caagatacag cgcagttcct ttgctagata
     4021 ttaaaaaaat taatgctcgt atcgaaccag gtgctattat cagagatcaa gtgtcaatcg
     4081 gcaacaatgc cgtgatcatg atgggagcga ttatcaatat tggtgcagta atcggtgaaa
     4141 acacgatgat tgatatgggt gctgtcttag gtggacgagc gacagtgggc aaaaactgcc
     4201 atattggtgc aggagctgtc ttagcaggtg tgatcgaacc agcatcggcc aaaccggtta
     4261 tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat tgttgaaggt gtccatatcg
     4321 gaaaagatgc agtcgtggca gcaggagcag tggttttaga agatgtggca gcagaaactg
     4381 ttgtcggtgg aatacctgca cgtgtattaa aaataagtga tgatcagaca aaagaaaata
     4441 cagcattgat tgctgcgtta cgcgaattat agaggagatg gagaaaaatg cgcgaaatag
     4501 atacctgtat tgtgggggca accggattag tcggccaaac gatgctcaaa gtgttagaag
     4561 aatatgattt tccagtagga cgattgaaat tattagcttc aaaacgttca gccggtaaag
     4621 tttgtgtatt caagaaaaag aaacacactg tacaggaatt gacagaaact tcatttgaag
     4681 gatatgattt agcgctattt tctgcaggtg cttctgtatc caaagagttt gcgccaatcg
     4741 cacgaagtca gggactattt gtcatagata attcctcagc atggcgggaa gattcggata
     4801 ttccattaat cgttccagag gtcaatctgg aagactatac attgaatcgg ctgattgcaa
     4861 atccaaattg ttcaaccatt caagcggtac ttccgcttaa agctttacaa gaagcctttg
     4921 gtatccagcg agtcaattat tcgacctatc aagcagtttc tggttccggg caaaaaggca
     4981 ttgaagatct tcatcgtacg caaaatgacg aggaaccagt tttgtatcct tataatatta
     5041 gccaaacggt gattccggaa atcgatcagc ctttggaaaa tgggtataca aaagaagaac
     5101 agaaaatgat aaatgagacc cgtaagattc tgcatcagcc tgctttgcca gtatcagcaa
     5161 cctgcgtacg tgtgccaatc gaaaatggac atggagtctc tgtggcagtt caactgcaaa
     5221 aagagtttac gacggaagaa attagagagt gtctcagcca gtttccaggg attcaattag
     5281 aagatgatct agcagcacat aaatatccga cgagcattct tgctagagga acagatatgg
     5341 tttatgttgg gaggatcaga aaagacactt ctttagagaa cggattgttg ttctatacga
     5401 ctgccgataa catacgcaaa ggagccgctg cgaatgccgt acagattgct gcagctttaa
     5461 tgcagaaaat agcggaggtg gcaagatgag taatacgctg aaagtagcga aattcggcgg
     5521 aacatcgatg tctcatgaag agcaattcaa aaaagtccgt acaattgttc tttcagatcc
     5581 caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt acgtcagatg atgacaaagt
     5641 aacagactta ctttatttga tttatgctta ccttcagcat catgttgatt ggacacccct
     5701 ttggaaaagg atttgtgatc gtttcattca agtaagagat gttcttgccc tgcagttacc
     5761 tattgaagat gaactagcac gcatacagaa acaactggaa gacgaaaaga taactgaaga
     5821 ttatttagta agtagaggtg aatatctgac tgctcaactg atggctgagt atcttggtta
     5881 ccagtttatt gacgcaaaag acctgatttt ctttgactat gatgggaaaa tcgatcagga
     5941 aaaaacagca caggaaatca caaaagcatt taaaagatat ccgaaaatgg ttgttccagg
     6001 gttttacggg gcgaaccctg caggaaaaga aaaattattg ggccgtgggg gaagcgacat
     6061 taccggcgcg atactggcga agatgttgca agcggaaagt tatgagaatt ggacggatgt
     6121 ttcaggaatc atgatggctg atccgcgaat catcgatcat cctaaaatca tcgaggaaat
     6181 ctcttttaga gaactgagag agatggctta tatgggggct aacgttcttc atgaagcagc
     6241 tgttttccca gttcaagagg ctgatatccc catccaaata aaaaatacca atgatccttt
     6301 cgctcaaggg acaaaaatat cgaatcacga aatagaaaag gaaaatggac tgacaggaat
     6361 agctggacgc aaggattttc tttccatcac tttattcaaa cgtcatatgt ccgatgaaat
     6421 cgggtttatc tggaaagcaa tgagcatttt tgctaaacat gagatatcaa tcgaacatat
     6481 tccttcaggg atcgacaata tcggagtgat cgtctcagca gaagcgattg ccgataagtt
     6541 atttctcatc acgaaagaat taaaagaaac acttggcgtg gaagaaatcg tcgtgatcga
     6601 agacttagct ttgatctctg tcgttggggg tccgcataaa gaattgattg gattatctgg
     6661 gaaagtgtta tcgatattga acaaattaga aattcgcaca tcgatcttat cacaaggagc
     6721 acaggagttg aatctgatca tcggtgtacc gaataatcaa tatgaaacag tagtgaaggg
     6781 tatctatgaa gggatggtga atacggatgc tgaaagaata tcgaatggca ctccatcaaa
     6841 ttcctgaaat cgggtttcat gaattcaaga caaaaaaata tttatatgaa caagtaaaaa
     6901 acagcgaggc cgttatccat gaaatcggcg aaacaggact actcctttat tttgataatc
     6961 agcaagaaga aacgattgct tttcgaacag atatcgatgc actgccagtc aaagaagaaa
     7021 cgaatctctc ttttgcttct aagcatgaag gttatatgca tgcatgtggt catgacggac
     7081 atatggcgat gcttttaggg ttgactgact ttataactaa acacattgag gatcttattt
     7141 ataatgtagt actgatctat cagccatcag aagaaatttc cggtggcgca caaagcgtta
     7201 ttcagtcagg attgttggat aaatatgaag tacaagcaat atttggcttt catctatggc
     7261 ctggactagc taagggagaa gttttctctc gaccaggacc tttgatggca caaagcagtg
     7321 aaacagacat tatcattaaa ggccagtctg cacatattgc ttctagtgaa aaaggaatcg
     7381 atagtctgga ggcagctgta cgatttttga agaatgttta tgattttgac cggcaattac
     7441 cggcagagtg gaatcatcta atgaaatttg gccaaatcca aggtggaacg atccgaaatg
     7501 ttttagctgg agaagttatt gtatccggaa gtatccgttc ttacagttca gatatgcaaa
     7561 attatttgaa gaggggatta gaccatttag cagaacagtt tcactctaca gctactattt
     7621 cttttcgcta taatgacggc tatccagctg taatcaacca tagaggctta tatgcagcac
     7681 tgttagattc aaacaaacta catgaattag atgctccagt tcttcaagcc gaagattttg
     7741 gcatatatac gcaacaatat ccatgtgtat ttttcttttt aggtgtcggt gatacccctg
     7801 ctttacatga tccgacgttc gattttgata tggatgtttt agaaaatggt gtagaatggt
     7861 acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat cgatagcagt tcaaaagagg
     7921 agcgagtaaa taatgatgaa tttatccaat cgatttagtc ctaggctttc tagaatagaa
     7981 gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga taccagatgt gatcaaactc
     8041 accctgggag aaccggattt tccaactcct gaacatgtca agcaagcagg aatagcagcg
     8101 attgaagaag acttttctca ctataccgga atgagaggat tagaggaact gcgggaagcg
     8161 gcttgtatat ttcagcaaca gcgttatgga ttaacttatg atccgcaaac ggaagttttg
     8221 acgacagttg gtgcaacgga agcgattgcc tctgcgttgt tatctgtttt ggaagaagga
     8281 gacaaggttc ttattccagc gcctgcttat tcaggatatc agccattagt ggaactagct
     8341 ggtgctgaat tgatcccgat agatacttct gacacagggt ttgtctgtca gccggaacaa
     8401 tttgaacgag catttgaaca atatggatca gctgtcaaag cagtcatttt aaactatcct
     8461 aacaatccta ctgggacgac attgtctgca aaacaattga aagcaatcgc tgaggtttta
     8521 aaaaaatatc cggtatttgt tataagtgat gaagtatatg ctgaattgac ttatagtgga
     8581 acgcatatgt ccatagctac ttatcttcca gaacaaacaa tcgtgataag cgggttatcc
     8641 aagtcacatg cgatgactgg ttggcgagtg ggctttattt ttgctcaaaa agcccttatt
     8701 gacgaactaa ttaaagtcca tcaatatctg gtgactgccg ctacaacgat gtcgcaaaaa
     8761 gctgcggctg aagcgctaat aaatggggtg aaagatagcg agaatatgaa agagcaatat
     8821 caaacacgaa gagactatct ggtccagcaa ttggcgcctt taggatttga agtgacccag
     8881 ccaaacggtg cattttactt gttttgcaaa ctcccagctg ccattcaaac tgactcttgg
     8941 cagttttgtg tagaattagc ggaacaggca aaagttgcgt gtatcccagg aattgcattt
     9001 ggcccagaag gagaaggtta tatccggatt tcttatgcta gcagcatgga aaaattacat
     9061 gaggcctgca aacggatcaa agacttttta acagaacagt gattcataga ttttatcaaa
     9121 aaaacaatcg ttaattttgc ggtatattct gcaaggagac gattgttttt taaatttagt
     9181 tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat gttaagttta aattgtttag
     9241 aatgatttta aaagaatatt caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt
     9301 gtaggtgcat ctcatggagg gcatcaatcg atcttggaac tattgacacg atacggtgaa
     9361 aatgtagata tcacgctatt tgaagcagga gactatattt ccttcatgtc atgtggtatg
     9421 gagctgtatt tagaagacaa ggtaacagat gtaaatgatg tacgtaattt cagaccagaa
     9481 aactttccgc aatccaatgt tcatatttta aataatcacg aagtaaaaac gatcgatgcc
     9541 gataaaaaaa cagtaacggt tactagcgca tcagataacg aaacaaaaga atatccttat
     9601 gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc tgccagtacc aggagcagat
     9661 ctagaaaatg tctacttgat gcgtggatac aattgggcaa ccaaaatcaa agaaaaatta
     9721 aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg gttacattgg gatcgaagca
     9781 gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc ttgatgtgat cgatagacct
     9841 ttaggcactt atttagatgc tgagatgaca gatattttag aacaacacct aaatgaaaac
     9901 ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta ctggtaacgg aaaagttgag
     9961 gcaatcaaaa caagtgaaaa agaaatccca gcagatctag tcattcaagc tgccggtgtg
    10021 aaggcaaata cagaatggtt aaaagggatc gtcgacttag atgaacgtgg ctggatccaa
    10081 acaaatgaat acttgcaaac gaatcttcca gatgtgtatg cagtaggaga tgctacactt
    10141 gcttattcta tcccagcacg caaaaaaatg ccgatcgctt tagcgactgt tgcacgtaga
    10201 gaagcacgtt atgtagtaaa acatcttttt gaagaagtac caaacaaacc gttcggcgga
    10261 gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt ttgcagcaag cggattgaat
    10321 agctttacag cagcaaaagc aggagtagat gtacgagtgt cttattatga agatacgtta
    10381 cgaccaaaat atgtaccaga agaacaagga aatccaaaag tgtccatcca attattctcg
    10441 gatccaatga cccatcaatt gctaggagga gcagtattat ctacctatga tgttacaggt
    10501 caaggaaatg tattggcact agcgatccaa aacaaactga ctttagaaga actagcagaa
    10561 gctgacttct tcttccagcc aggctttgac cgacaatgga gcatgttgaa tctagccgct
    10621 caacaagcgt taggagaaga accatttgta gaataagaaa acgaaatatt tgaaaagagg
    10681 ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    10741 gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc gtttattttc
    10801 aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa aagttagacc aaaaaatcta
    10861 acttttggag gtcggtattt tttatggtta aatatacttt tgaacttaaa ttaaagatcg
    10921 ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa aatccagatc aagagattga
    10981 agcggaaatg cttaaa
//
LOCUS       DAFBYI010000089        10658 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFBYI010000089 DAFBYI010000000
VERSION     DAFBYI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.089"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            35..367
                     /locus_tag="LLD04_002081"
     CDS             35..367
                     /locus_tag="LLD04_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649000.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            368..961
                     /locus_tag="LLD04_002082"
     CDS             368..961
                     /locus_tag="LLD04_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649001.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            983..3007
                     /locus_tag="LLD04_002083"
     CDS             983..3007
                     /locus_tag="LLD04_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK5649002.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            3007..4206
                     /locus_tag="LLD04_002084"
     CDS             3007..4206
                     /locus_tag="LLD04_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5649003.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            4222..4866
                     /locus_tag="LLD04_002085"
     CDS             4222..4866
                     /locus_tag="LLD04_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649004.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            4877..5683
                     /locus_tag="LLD04_002086"
     CDS             4877..5683
                     /locus_tag="LLD04_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649005.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            5706..5935
                     /locus_tag="LLD04_002087"
                     /pseudo
     CDS             5706..5935
                     /locus_tag="LLD04_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5956..7029
                     /locus_tag="LLD04_002088"
     CDS             5956..7029
                     /locus_tag="LLD04_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBK5649006.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            7073..7342
                     /locus_tag="LLD04_002089"
     CDS             7073..7342
                     /locus_tag="LLD04_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649007.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            7618..7893
                     /locus_tag="LLD04_002090"
     CDS             7618..7893
                     /locus_tag="LLD04_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649008.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            7935..8357
                     /locus_tag="LLD04_002091"
     CDS             7935..8357
                     /locus_tag="LLD04_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649009.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            8377..9969
                     /locus_tag="LLD04_002092"
     CDS             8377..9969
                     /locus_tag="LLD04_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649010.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            9990..10325
                     /locus_tag="LLD04_002093"
     CDS             9990..10325
                     /locus_tag="LLD04_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649011.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            10471..>10658
                     /locus_tag="LLD04_002094"
     CDS             10471..>10658
                     /locus_tag="LLD04_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649012.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         3924 a         1628 c         2233 g         2873 t
ORIGIN      
        1 taaaaataca aaatataagg ggtatggtta tattatggat aaatacattc atatgaaaaa
       61 tcctccaaga tttagtgggg aatggaagtt tttcgccttt ttctttttag atctattaac
      121 gcttggtggc tgcttttttt gggcgaaaat agtcaataat cttgtccact taccactctt
      181 ttttgcactc ttgaattatg gattatggct tgcgatagcg ttagtgattg tttggcgacc
      241 ctataataac cctggtcaac gccaaatttt tgcgattatc gaatctattt tctttacgga
      301 taagaaccac tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
      361 tgaataaatg cgagtcaaag gacaaaattc agttgataag aaatccattt tgcagtttgg
      421 aaaaccagaa gagcgctcta tcttggactt atttccatat aaatcctgta cagaagaggg
      481 agtcttaatt actagagaag atcattttca gcgttattat cgtgtcgttt ccactgacgt
      541 ggagggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
      601 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
      661 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcac taggaagaaa
      721 tctacagcga ttaagaaaat atgaaaagaa attagtagag gagatccagg aattaaaacg
      781 ggcagaacaa gaacgtcctg acttaagctt ctttttcgtt attgaaggaa agacaatcaa
      841 ggatttacaa actagaaatc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
      901 actctacaaa aaagaattga ttaagattct ttatcgaatg aacaacatga atgacgaata
      961 aaaaagatgg aggaaatgat tcatgctaga tgtgataaaa acagcttttt caacgcaaag
     1021 taatcaaaag gtgattgatc cattaaaaca acttggtaat cgtgaaatag agattttaaa
     1081 atcaaaaggt tatgatttag atttcttaaa acaagtgatg ccacaaggtg ggttgaattt
     1141 tgatgaagaa tatgtgacct atggaaatgg ttattcacga tgtgtgacag tttatcatta
     1201 cacaaaaaat cctactcttt tttggttggc caatctcatg aacaatcctc atacagtggc
     1261 cacactagat gttagcacag atgaaaagca aaaagtgatt gatcaaatca accgcgcatt
     1321 agatgaatta aatgatcgat cagataatga acgaaaagcg actgaccgaa ataaaagtgc
     1381 ggaagaatac ctggaactag cggaatatgc ccgaaaattg acttctaatg gggaaatttc
     1441 taaacaagtc aaagtgagag tacatgttta cggaaatacg atccaagaag tggatgaacg
     1501 ggcgcaggaa ctgatcgaaa atctaaaagg gatggattat cgtgcggtga cttatctgca
     1561 taaaaccaaa cgagattggc aatcgatgtt taccatgttc gatgaacaag ctgaatggtt
     1621 tggaaatatc aaagggcaat cacttccatc gttaaacatt ggcggaggat tcccgttcca
     1681 ccaccaaacg ctaaaagatc caggaggcac acaatatggg gaaaccatga caggcggccc
     1741 atttgtctgg aatgccacgt ataaaaatag tgtacgttta tctaccaata ccttgcttct
     1801 aggaatgatg ggagctggta aatcgaccgt tcttaaaatg atcgcggaag cccatttagc
     1861 tgcaggagac tttatttggg gctttgaaaa gggaaaagac tttattcctt tcttaaagga
     1921 atataacggt attatggtgc gtttagacgg atctgacggg atgatcaatc ctttagagat
     1981 ctttgccaca cggacgtatg atgaagcatc cagtttatat gacgatggtt cagtcaaaga
     2041 tttgaagatc aatgaagcag cgtcctatca aacgcactta gacaaagtag tctatcaggt
     2101 acagttagtg agtcctcagc taaaaggaac gatgaaagca gaattcaaga cctacttgaa
     2161 tcgtttttat gaagagtatg gaacagtacc acgcggtttt acttcctcaa attcgcgttc
     2221 aaatactgat acgcaagtca cagggaaaga tccagaagct tatccgacgt tcaaagaatt
     2281 tttagccttt ttaggacaac tggaactacc aggggcttcc caagagaaaa agaatcggaa
     2341 agaagaaatg gagtctatcg tggaatcctt atgtgagact tatggcatga tttttgatgg
     2401 tcatagtacg attcgacacc ttgaccaaca acagttggtc ttttttgaca ttgactctat
     2461 tacaggccta gatcgagggg tacaacaatg tcagatgtac atggccatga cgttgatttg
     2521 gaatcaggca ttgattcaag gacgaagaca acgacgctta attaaagaaa aacaaatgga
     2581 agaacaagac ttcaaacgct tcttggcgat gattgatgag tgccataatg cgattaatcc
     2641 agatttcatt gaaacggtaa attatgtcac aaacttccaa cgagaaatgc gtaaagtaga
     2701 agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa atatgtcttc
     2761 tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga tctttatgcg
     2821 tatggatgaa tctattttac gtcatataga aggattagtt ggtaaatcaa tgacgaactc
     2881 agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt ttggctcaaa
     2941 agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg
     3001 aaaataatgg aaaaccaaaa tgaaagttta atcaaacagt atgttaagcg tcgtgcaaag
     3061 cggcgcttat ttttgtgggt gtttggtact tctgcaggtt tgattactat attgatcact
     3121 gtgtttgtga ccctttttct aatacttgcg gcaggttcta ttgataattc tgattcaagt
     3181 agtggtggcg aagcttttac aggagaatat tcggaaggct tacccattta taaagaaatc
     3241 aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaag
     3301 tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc gtttggtacg
     3361 tcggcgtccg caagaaatga tctcaattat tttgggatta cgtggtttga tgggtgtctg
     3421 tttcctaaag gaactgcgcg tggaattggt ggcattgaag gtggctggta catgaagttt
     3481 cccaactcaa aagctgcctt ctcctattac ggctttatgg tagccacaca atcaaatttt
     3541 aatgcttgtg tcggaaataa aagccctggc gcaagcttat tgatcttagg acgaggaggg
     3601 tatgccgccg caggaattac tgaagacagt gcctattata aaaattgtat gtctattatt
     3661 aatacaaata aattgatgga gtacgatgag tttgcgatta aacactggag agaaggcggc
     3721 ggaactggtg gtacgattac aggaagctgg ggcaatccat tcccaggaag tagcttagat
     3781 aaaaattcgt tcagtggagg acaacttttt gggaaaaatc caggaggaga attccgacct
     3841 aacgggttcc atgatggact agactttggt tcagtcgatc atccaggaag tgagattcat
     3901 gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttgggtgcg
     3961 tgtgtgatcg tgattaatta cgatggcttg aatatggtgt atcaagaatt tgcaaatagt
     4021 acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt aattgggata
     4081 cgagatacag cacacttgca tttaggattt actcgaatgg attggcgtca agcacaagga
     4141 catgcattca ttgatgatgg tacatggatc gatccgttac catttttgaa tagcagcaaa
     4201 aaatagatca ggagaaaaaa tatggaaaat aaaaccttaa aaatcgtctt aacagctgca
     4261 attagcgtta gtttaatcgg caatattgcg ttgttttggc agcttaaaga agaacaagag
     4321 acaaatacct ataaaattga agaagtggct aaaaacgcgt ctgataagct caaaaaaaat
     4381 tcttctgaag cacaggcgaa gattgacaag ttaaatcaaa ccatcaatga gaaaaaccaa
     4441 gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt aactgaagca
     4501 caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat
     4561 gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct
     4621 gtagacgatg atcaccaaca tgatgattac ggaagttact cttttacatt aggtgacgtg
     4681 caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat
     4741 tatacaatcg gcaatcctca attcaaagat gtcaaaccac aaaaaataaa aggtggatta
     4801 actgtcactg aaaaacaagt agatggtcaa tggaaagtca cagactttag ttatttcact
     4861 cgttaggagg aagaaaatga gtaagaaaaa aggagcaaga caacgtttta aaaaacgaaa
     4921 aagttatctt agatggctgg ggatctttat gattctcggc tttttgattc ttgctagtcc
     4981 gatttacaca cataaaaagg tagacatacc agatgaagta agcacgaacg catttttaaa
     5041 cacgaaggaa ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga
     5101 atttgcgatc gacgatgaag atcagagctt attaaaagaa ctagctaatc ttaattatcg
     5161 tgtagaagta aagacagcta aaggggatta tcaagcaata aaagccaaag tgatcaaggt
     5221 gtctgatgat tattttgtag tgaagttagc acatgtacca gaagaatata ttgctatgcg
     5281 attaactatt attcctgaaa aaattgatcc aaaggtggat atgcaagaac cgcaagatct
     5341 gattttttat attcacgaag ataaggtcaa agatcacgtg aaagacgaag attatgaaca
     5401 acatgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag cacaaaaaca
     5461 aattgaatcc ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga aactaaaaga
     5521 tcaattgcct tatcaagtcg cagaagatca ggaaaatact gaaaataaga ttaatagtta
     5581 ccagcaagaa attgaaacat cgaaaacgca aatgaaagat caagaagaga gcattcaaaa
     5641 acttcaagaa aaaattgatc gtatgacaaa agaaaatatt tagaagaata ggtggagtaa
     5701 aagaaatgaa gcgagcgaac ttggtcggca aagatacttt cgctgagcag aaagtattga
     5761 ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg aattatctaa
     5821 cacaacaata tctgagttga agaatggtag aaagagttta gataccttaa aattcagatc
     5881 atttgaagct ctatatatag ctgctaaaaa atattttaca gctgataata aataaaaaat
     5941 taaggtgggt tctatgtgaa aaaaacgtat caaaagtttt tatggattag ctatatcgtc
     6001 ctatttatcg ttgggtttct cgtcagtgtg tccgtgatcg tcggaacgct ttttcttttg
     6061 ttctctgacg aagatctggt acttaagggg ttaatgagta cgttatcgtg tgtcatgtta
     6121 ctttatttat gtatacagat caacgaaatt atgccccctc attggcaaga aaagaagaaa
     6181 gaatttaagg aagaaatcga aataaaatcc aatgactgga ttgaatacac atggcttcct
     6241 ctgaaaagaa atagcaaacg aaaggaattt tgggcattgc aattatggcc aataggaaaa
     6301 ctttttattt atatggcttc acttatggtt actttaatcg gttatttggt ggtggtctat
     6361 ggggtggcct attacttgtt aagtgagcca aaacagatcg cggcgtcaaa tattttcacg
     6421 gcagtaatga cattaatgtg tgtcatgtgc ttttctattt ttagttactt actcgtccaa
     6481 tttatcaggc aaaatttaac aatgagtaaa cttttcaaaa gtttattgga cttacttatc
     6541 ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa aacggtagag
     6601 gggataaaga acttcttaac gacgattgat caatttataa atggatatat gtatttgttt
     6661 gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat taaaagatca
     6721 atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga gtattacgtt
     6781 gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag tatccatgaa
     6841 gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa aacaatcgac
     6901 cgaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata tggcattggg
     6961 aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt aggaaaagtg
     7021 gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga aaatgattct
     7081 tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga aggagcagtt
     7141 attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta cgatgaacat
     7201 tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt cgattcgaga
     7261 gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg agtgggaaca
     7321 attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa agagcataag
     7381 gaaacgtgta cccactttca aaataaagtg ggtagatagg ctgtgcctat cacgtttctg
     7441 aaaaaaataa atgaccccaa aattttcaat ttatgtgtag gtttatgggt agcaaaaata
     7501 tgaatgtggg cagcaaaaag ttcatttgtg ggtagtaaaa ttcaaatgtg tgtagcaatg
     7561 tgtgtagcta cacacattgc tacacacgct ataacatata gtgaaaagga gatatttatg
     7621 gaagtaaaaa taagagatat tgatgaaaaa acgattcaaa gaattgaccg aagatgtatt
     7681 gaattaagtg aacgcaccgg gactaaatgg agccgaaatc gatacctaaa agttttgatt
     7741 gaaaatgatt ttgaccatga acttatggat catagaaaag atcaatttga tcggttgtta
     7801 gaaaaatttg tggatattca aacttataat acgaaaattt tagaagaata tattgcaaca
     7861 aataatcaat taatcgcctt gttaattgaa taataaaaat agtattgtga atttataaaa
     7921 ggaggaaatc aattttggca aaagcaacaa gaaaacatgt atatctttac gatatgcata
     7981 tcgaagtact tgatgaaata atttctactc atgaagatat atctaattat agtgaagcag
     8041 tcagacattt gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa
     8101 ttaaattgaa tgcaatggga aaagaaattt ctatattaac cgaaatggtt tcagagtttg
     8161 cggatcttca tttgaaagat acaggaatac taattggtac aaattcaact gtctatcaag
     8221 aagcaaaaga gcgtgtggaa gggaaaataa aagcgaatca aacgaaaaag tattctccta
     8281 agatcaagaa accaataaat gaatctgttc atgccagagt agacccgttt gatccaacaa
     8341 agtttttaaa aagataaaaa gtagaagggg aacatgatga ataaatcgcc tgcagtcatt
     8401 ctcacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg
     8461 acaagagaaa aagcgctgga agaaaaggaa cgacgcacgc cggacgaaga acaggaactg
     8521 caacgaatca agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggag
     8581 agtaagacag ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt gatggaacat
     8641 ggaatcaatt ttgacaagtt agaggaacaa gattttacca aatatctttc ttatatgacc
     8701 cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa agaataccaa
     8761 cggttgaacc aagcaatgaa aaaatatcaa acagagccac ataaaaaagc ttcaggaaat
     8821 cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc
     8881 aaacaaatct tccaaagagg gcaacaaaaa ggctcaatca tttatcaaga tgttgtctct
     8941 cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga taccttagat
     9001 gaagaaggat taaaagcagc tggaagaaaa atgatggaga cattattcga gaaagaacaa
     9061 ttgaatgaaa caggttactg gatggcaacg attcaccgta acactaagca tatccatatt
     9121 cattttgcag ttgttgaaaa agaaaatacg cgtaaacctt acacggaggt tgaaaatggt
     9181 gtgagttata tcgtaccgaa aggtaagaga agacaagcaa cattagacgc aatgaagacc
     9241 aattatgtac atgagttaga acgtttttct tatgaaaagg atcgaatgat tggtatggag
     9301 aaacgaaatt tattgacgcg gaaaagtgat ttgcgtaaca ccttaacaaa agtggtaaaa
     9361 gagccagcgc gttacgatcg acacgcaatg aacttattaa atgaagtata taaaacccta
     9421 cctgacaaac gttcggaatg gcattatggg gatgaaaaac gaaccaaact ttcaccatac
     9481 acaagagaga aactcgatga gttaaccaaa tatgtgttaa caaaagaacc tgaatatcaa
     9541 gaatatatac agatttcaaa atcattgaaa gaagaagcaa aaagtcttta tggggaaagc
     9601 aagagagaat caaaggatgc agaaaaaaat gctctctttg atttgaagaa gcgcacagga
     9661 aatgccattc taacagaact gaagaaacag gatagtcaaa taaaaccttt aatagaaagt
     9721 atggatcaga tgcaatctat ttacaatgaa cgatcctttc gaacgcttaa acagttacca
     9781 acgaatgaat acaaatatag ttacgaagaa ttcatgaagc gcaggaaaca attccaacaa
     9841 agagttgtca cgcaacggag tttgcgtcaa ttaaatcgaa cgattaatgc ccatgaagaa
     9901 aaataccacg caagtaaagc ctacgaagag caacaacgaa gaattcaaat ggaacaagaa
     9961 agacagtaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa tggaaagtta
    10021 tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta gaaaaagagc
    10081 atttgtttca gataagagaa atgttgagaa acgaacagtt tgatccaaat caatttttag
    10141 tcgttgagta ctcagctact ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt
    10201 ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg gaaatgaatg
    10261 aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca ctcacagaat
    10321 gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtaattgt ccaggactct
    10381 ttttttcaat gaattgtttt ccgccaattc aatgagtggt gcgatttgcg actacattat
    10441 atcaaaagaa agagaaggga ggaagaagaa atggcttatt caacgattgg agttgttgtt
    10501 gaaaaaagca gagacaatct cgtttttgtg acagaaattc aaacgggcag agcttttgtc
    10561 gtcacagaca aagcggcaaa agcgtatcaa agtggcgata ttttaacgtt aaatctgact
    10621 acgaagacgt tcgtcgatgc ggcagaagat tatccatt
//
LOCUS       DAFBYI010000090        10597 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFBYI010000090 DAFBYI010000000
VERSION     DAFBYI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.090"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..691)
                     /locus_tag="LLD04_002095"
     CDS             complement(<1..691)
                     /locus_tag="LLD04_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK5649013.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFER"
     gene            complement(741..950)
                     /locus_tag="LLD04_002096"
     CDS             complement(741..950)
                     /locus_tag="LLD04_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649014.1"
                     /translation="MDVLREYSSLFTEVYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1227..2500
                     /locus_tag="LLD04_002097"
                     /pseudo
     CDS             1227..2500
                     /locus_tag="LLD04_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2607..3638)
                     /locus_tag="LLD04_002098"
     CDS             complement(2607..3638)
                     /locus_tag="LLD04_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649015.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3645..4475)
                     /locus_tag="LLD04_002099"
     CDS             complement(3645..4475)
                     /locus_tag="LLD04_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5649016.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4472..5287)
                     /locus_tag="LLD04_002100"
     CDS             complement(4472..5287)
                     /locus_tag="LLD04_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5649017.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5284..6108)
                     /locus_tag="LLD04_002101"
     CDS             complement(5284..6108)
                     /locus_tag="LLD04_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK5649018.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6098..7198)
                     /locus_tag="LLD04_002102"
     CDS             complement(6098..7198)
                     /locus_tag="LLD04_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5649019.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7376..8161
                     /locus_tag="LLD04_002103"
     CDS             7376..8161
                     /locus_tag="LLD04_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK5649020.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8194..8577
                     /locus_tag="LLD04_002104"
     CDS             8194..8577
                     /locus_tag="LLD04_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5649021.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8653..8784
                     /locus_tag="LLD04_002105"
     CDS             8653..8784
                     /locus_tag="LLD04_002105"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5649022.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            8753..8989
                     /locus_tag="LLD04_002106"
     CDS             8753..8989
                     /locus_tag="LLD04_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5649023.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9067..10038)
                     /locus_tag="LLD04_002107"
     CDS             complement(9067..10038)
                     /locus_tag="LLD04_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK5649024.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3140 a         2473 c         2021 g         2963 t
ORIGIN      
        1 gccgctcaaa cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt
       61 cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag
      121 cagtcgccac atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat
      181 gttcaggatc ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa
      241 cgtaggcaac cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt
      301 aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc
      361 atgaactatc gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca
      421 tttgttctaa caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt
      481 tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg
      541 gtgctgcttg ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc
      601 gtttcaagca caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg
      661 tcacaaagga atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct
      721 cccgcagttc agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa
      781 tgttcagcat ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct
      841 gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc
      901 agtaaataga cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa
      961 tcatcgtccc atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa
     1021 aacaagcaaa agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa
     1081 aagtcaaagg tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta
     1141 tcagtccttt tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc
     1201 acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag
     1261 gaatcaaaga tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca
     1321 acagtatcgt tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac
     1381 aggtgaatca acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca
     1441 ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg
     1501 gctgtcacac ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca
     1561 aacaagtaca ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt
     1621 ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca
     1681 tgacaaacgt gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat
     1741 tgaaaaatca agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac
     1801 tggatattct tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac
     1861 gacgtgcacg tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc
     1921 tgataaaaga gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc
     1981 acctcattcg cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg
     2041 atccaataca agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac
     2101 gacaggacac gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt
     2161 taaccgaaag tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt
     2221 atgcttacta tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc
     2281 agcttgtaaa aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt
     2341 ttataaacta tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa
     2401 tagaaaatct gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag
     2461 aaccagaatt ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag
     2521 cagaaaatca attctacctt agatgttctt atatcactct atcagtccta tttgacaaag
     2581 agccagaaat gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt
     2641 tccacaggga ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct
     2701 tcccgatcgt tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac
     2761 cgttgaatgt tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga
     2821 ggaaccggtt caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta
     2881 gcggaaaact cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg
     2941 ataaattccg caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc
     3001 tgagccaccc gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct
     3061 tggtcatgag tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc
     3121 cgctgcagct gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg
     3181 actttcggtt cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa
     3241 agaaactgga catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa
     3301 accttttcct gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt
     3361 tcaaaaacag tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga
     3421 tccttcgtag gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt
     3481 tgttccaatc ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt
     3541 aaagtaacca gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca
     3601 tcaaaaaaca gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag
     3661 ggcttctttt tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt
     3721 cgataagtca gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc
     3781 atcacagcgc tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc
     3841 gtctgaaatc gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa
     3901 aaaatcatca gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata
     3961 ataatcgtag acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta
     4021 attaagcgca tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat
     4081 gcacctacca acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc
     4141 gttaccaaaa tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga
     4201 ttcaaacgag ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc
     4261 gtcaccaaga ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg
     4321 tccgtcagca gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac
     4381 catttagtgg agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca
     4441 aagcagaaca aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt
     4501 gaataatcaa cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta
     4561 aattaggctt ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat
     4621 ttacattact gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata
     4681 agatgacaaa ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag
     4741 catacgctga tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct
     4801 tcatgctggg atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg
     4861 tcaatcccca ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca
     4921 cacctacatt ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag
     4981 ctgccaaatt ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc
     5041 ataaacataa aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta
     5101 cgctgttaac aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca
     5161 atgtccactg gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca
     5221 gcagtaaaaa tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct
     5281 ctttcatccc ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa
     5341 taaccagcaa aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg
     5401 gcgtttattt gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg
     5461 cagtccgtgc gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag
     5521 ccattcagga aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc
     5581 atgagaatcg gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa
     5641 aaagtctggc tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg
     5701 gtaagtgtcc tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat
     5761 gcgatcaacc aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc
     5821 ggcggctcct gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga
     5881 tcgctgagca tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag
     5941 tacttcgtat aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc
     6001 ttgctggtgc tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg
     6061 acagccgtct agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct
     6121 ccgcctgcca aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa
     6181 ctggcaccgc cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca
     6241 gctctacctt gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag
     6301 ataaaatata ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta
     6361 aggttgtata cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg
     6421 agtcaatttg gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt
     6481 cacctttttc aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca
     6541 tttttttgta gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg
     6601 ccgcagccaa tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt
     6661 gatagtcacc ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat
     6721 cggtgataaa ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg
     6781 cccaatccgg aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt
     6841 cagccaacgg accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt
     6901 ctgattcaaa cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc
     6961 cgtattcttt ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc
     7021 ccaagctggc aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt
     7081 ctgccgcagc cgattgcttc gttgcttcac ctgagacgca gcctgtcagc aggatactta
     7141 ctgttgctgc acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc
     7201 attcttctcc cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt
     7261 cttgaaaagg tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt
     7321 cccgttaacc ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca
     7381 gtattatgat cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa
     7441 ctatttagcc tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc
     7501 atctaccgga aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt
     7561 aaaagaaacc tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca
     7621 agaagagcgg atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat
     7681 tcatcaaaat ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca
     7741 aactaccaag gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga
     7801 tattttgact cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc
     7861 ggagcatttt gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc
     7921 gatggagtta aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc
     7981 cgttcctttg tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt
     8041 tgccgaagat tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt
     8101 agaaaagttg ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg
     8161 attcctatat aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa
     8221 gaggcaccca tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt
     8281 tgccgatcct tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac
     8341 agcttgcagc gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa
     8401 tatttgcatg cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat
     8461 ttacgaggac agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg
     8521 ggaatggctc gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg
     8581 caggatcaag gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag
     8641 gaagaagttt gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac
     8701 caagctcccc tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac
     8761 acacctttcg gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc
     8821 tggtgatgaa gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact
     8881 ttatcatttt aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca
     8941 gttttttatt gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac
     9001 ttggagcttg gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac
     9061 caagggttag aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg
     9121 attgagccag tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac
     9181 tgacttcagc aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat
     9241 ccataagata aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag
     9301 caaatgagga accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg
     9361 cgctggcgtg tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata
     9421 gcagtggtac ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc
     9481 aatcgtcagc agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg
     9541 cacttcgcag ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt
     9601 atctaagccg gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa
     9661 tgtatccaca tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc
     9721 cagtacggta ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc
     9781 ctgaaccagt gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac
     9841 agcatccttc tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt
     9901 ttcaatctgc ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc
     9961 ctttaaataa acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct
    10021 taatactgtt ccttccatat agatttttga attacacact agtttaaact aaaacacaca
    10081 aggaaaaact taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat
    10141 agactggtca aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat
    10201 tttaatattc taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca
    10261 ctatatcact agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt
    10321 aaatggtcat tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac
    10381 gctgagagta aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt
    10441 tatttcagat taccttaaat gattccattt actttttatc tcattttttc ctactagtta
    10501 gtatcaaata ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca
    10561 gctaaaacct tgtcattaca gcaaaaaatc acgttat
//
LOCUS       DAFBYI010000091        10437 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFBYI010000091 DAFBYI010000000
VERSION     DAFBYI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.091"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            91..309
                     /locus_tag="LLD04_002108"
     CDS             91..309
                     /locus_tag="LLD04_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649025.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..1110)
                     /locus_tag="LLD04_002109"
     CDS             complement(598..1110)
                     /locus_tag="LLD04_002109"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK5649026.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="LLD04_002110"
     CDS             complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="LLD04_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK5649027.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3563..4006)
                     /locus_tag="LLD04_002111"
     CDS             complement(3563..4006)
                     /locus_tag="LLD04_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK5649028.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4007..4795)
                     /locus_tag="LLD04_002112"
     CDS             complement(4007..4795)
                     /locus_tag="LLD04_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5649029.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4792..5733)
                     /locus_tag="LLD04_002113"
     CDS             complement(4792..5733)
                     /locus_tag="LLD04_002113"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK5649030.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5910..6497)
                     /locus_tag="LLD04_002114"
     CDS             complement(5910..6497)
                     /locus_tag="LLD04_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK5649031.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6718..8025)
                     /gene="obgE"
                     /locus_tag="LLD04_002115"
     CDS             complement(6718..8025)
                     /gene="obgE"
                     /locus_tag="LLD04_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK5649032.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8319..9329
                     /gene="gap"
                     /locus_tag="LLD04_002116"
     CDS             8319..9329
                     /gene="gap"
                     /locus_tag="LLD04_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5649033.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9414..10349)
                     /locus_tag="LLD04_002117"
     CDS             complement(9414..10349)
                     /locus_tag="LLD04_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5649034.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3384 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
      721 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
      781 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
      841 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
      901 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
      961 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     1021 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     1081 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     1141 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     1201 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     1261 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     1321 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     1381 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     1441 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     1501 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     1561 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     1621 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     1681 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     1741 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     1801 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     1861 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     1921 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     1981 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     2041 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     2101 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     2161 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     2221 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     2281 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     2341 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     2401 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     2461 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     2521 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     2581 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     2641 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     2701 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     2761 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     2821 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     2881 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     2941 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     3001 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     3061 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     3121 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     3181 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     3241 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     3301 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     3361 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     3421 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     3481 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     3541 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     3601 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     3661 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     3721 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     3781 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     3841 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     3901 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     3961 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     4021 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     4081 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     4141 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     4201 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     4261 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     4321 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     4381 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     4441 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     4501 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     4561 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     4621 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     4681 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     4741 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     4801 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     4861 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     4921 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     4981 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     5041 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     5101 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     5161 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     5221 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     5281 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     5341 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     5401 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     5461 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     5521 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     5581 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     5641 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     5701 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     5761 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     5821 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     5881 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     5941 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     6001 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     6061 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     6121 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     6181 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     6241 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     6301 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     6361 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     6421 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     6481 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     6541 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     6601 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
     6661 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
     6721 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
     6781 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
     6841 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
     6901 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
     6961 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
     7021 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
     7081 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
     7141 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
     7201 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
     7261 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
     7321 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
     7381 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
     7441 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
     7501 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
     7561 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
     7621 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
     7681 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
     7741 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
     7801 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
     7861 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
     7921 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
     7981 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
     8041 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
     8101 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
     8161 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
     8221 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
     8281 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
     8341 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
     8401 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
     8461 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
     8521 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
     8581 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
     8641 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
     8701 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
     8761 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
     8821 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
     8881 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
     8941 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
     9001 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
     9061 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
     9121 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
     9181 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
     9241 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
     9301 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
     9361 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
     9421 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
     9481 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
     9541 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
     9601 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
     9661 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
     9721 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
     9781 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
     9841 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
     9901 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
     9961 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    10021 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    10081 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    10141 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    10201 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    10261 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    10321 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    10381 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataac
//
LOCUS       DAFBYI010000092         9918 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFBYI010000092 DAFBYI010000000
VERSION     DAFBYI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.092"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(31..297)
                     /locus_tag="LLD04_002118"
     CDS             complement(31..297)
                     /locus_tag="LLD04_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649035.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(290..613)
                     /locus_tag="LLD04_002119"
     CDS             complement(290..613)
                     /locus_tag="LLD04_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649036.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(625..1881)
                     /locus_tag="LLD04_002120"
     CDS             complement(625..1881)
                     /locus_tag="LLD04_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBK5649037.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(1878..2273)
                     /locus_tag="LLD04_002121"
     CDS             complement(1878..2273)
                     /locus_tag="LLD04_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649038.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            complement(2316..2567)
                     /locus_tag="LLD04_002122"
     CDS             complement(2316..2567)
                     /locus_tag="LLD04_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649039.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            complement(2567..2962)
                     /locus_tag="LLD04_002123"
     CDS             complement(2567..2962)
                     /locus_tag="LLD04_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649040.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            complement(3317..4318)
                     /gene="ccpA"
                     /locus_tag="LLD04_002124"
     CDS             complement(3317..4318)
                     /gene="ccpA"
                     /locus_tag="LLD04_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK5649041.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            4528..5631
                     /locus_tag="LLD04_002125"
     CDS             4528..5631
                     /locus_tag="LLD04_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5649042.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(5694..6296)
                     /locus_tag="LLD04_002126"
     CDS             complement(5694..6296)
                     /locus_tag="LLD04_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5649043.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(6299..6739)
                     /locus_tag="LLD04_002127"
     CDS             complement(6299..6739)
                     /locus_tag="LLD04_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK5649044.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(6864..7802)
                     /gene="galU"
                     /locus_tag="LLD04_002128"
     CDS             complement(6864..7802)
                     /gene="galU"
                     /locus_tag="LLD04_002128"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5649045.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(7817..8842)
                     /locus_tag="LLD04_002129"
     CDS             complement(7817..8842)
                     /locus_tag="LLD04_002129"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5649046.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(8886..9716)
                     /locus_tag="LLD04_002130"
     CDS             complement(8886..9716)
                     /locus_tag="LLD04_002130"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK5649047.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(9731..>9918)
                     /locus_tag="LLD04_002131"
     CDS             complement(9731..>9918)
                     /locus_tag="LLD04_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK5649048.1"
                     /translation="TVEIAEVGIPQIKIPVKTGRNVAIIIEVAAMNFRARTMGFDATK
                     TFEERLSRLIEENSGND"
BASE COUNT         2616 a         2096 c         1708 g         3498 t
ORIGIN      
        1 attttcacaa aatttttcta tataacttta ctaacgaaac tgttttaatt tcttttcttg
       61 tctttggaat aattcagtta gtttttctgc aaattgccct tttactgatc ctttcaagtg
      121 tacatgtcgg ttagaaaatt cttgagcagc ttcttctgca gtcatttggt agttttgatc
      181 tgtcaatcgt ttttctgccc aaatttgctg agatatcccc ataccaactg aacgtacatt
      241 tgatcgatgt agatttttct ctaattcttt tagtaattgc attaaaaatt cattcataag
      301 atgatttctc catagttatt tgtctgattt tttgacgata ggaacgttct agttcatctt
      361 gctgctgata gagttttcgt cgttctactt gtagggcttg ttgataatgc tcctgataat
      421 tcgaataact tcgtcttgct tgaaacagag gctcattatc taaagctaag ttctttaata
      481 catgatgcag tcgctgctcc tcttgctcca tcaatcgtaa tccttgattc ttaaagtgga
      541 tcaattcatc ttcttgagct tctaattgtc ttcgttcact tcgataatct ctttccaatt
      601 cttctagttt catctacttc tcctttaatt gaattgctgt gctaattctc gatctcgtgc
      661 cacgatttca ttgattttgt cttggatctc cttcgccaat gtatcaaagg cctcccccat
      721 acgtcttgcc ttattgattc gtagcagata gacttctttc gcagaatcga ctaagttttc
      781 ttttgtggcg cccacactag ctaatgcttc tatgatttcc tgttcagtca acgaaggggc
      841 tacaacacgt gcttgccgca agccttcttg ccattttttc tctagctcaa aaattcctct
      901 ttgattgatg cggataactt cttccatagc gcttttcatt tttgccgata acttttccac
      961 tactaaccta gcttgtgtgt catctaagta aatttgttca ttaactgtca gctggccgcc
     1021 acttaatcgt agttttttac gtatgttgtt cagttcttgc aactgatagg aaaacaactg
     1081 ggtcgcttgg ttttctttct gttgttttcg agctttaaca ttgttttcat tttcaggaat
     1141 aatcagcttt ccattttcat caaacttcca agaggttaaa ctgtgggaag caagcagatt
     1201 caagaatgct gtttccatac tgatccgaac ttcagcacct gtgccattac caccaaaatt
     1261 accgataaaa tcggcgtgat ttcggtaatt ggtgagcata ccgggatttt cttccaccca
     1321 tttttttgct tctggtgata agagatcata cgggtccggc ccgttgaagc cgacattttt
     1381 ccattgtttc tctgctgcta catataatgc taagaatcca cctaatgaat ggccggtagt
     1441 agaaatagca gcttctccat attccttttt tacttcatta gcaaatgcaa tcgctgaatt
     1501 gatttgcccc gctgttttca atccactaac ggataaaatc ttgtttgttg ttgctttttc
     1561 agaaaaactt gctcctggtc tattcccgta gagattcatg atacttcttg catccgtttc
     1621 caaatctttc caatcactta agttcgttcc tgcaaaagca atcactactt gagaaagatc
     1681 cactttaccg ttcttgattg gcgctacaga catcgcctgc atgccgtcca atgagtcttt
     1741 gatttgtagg atttgaaatt ccataccagc aaattcttta acagagttct ttttgtatcc
     1801 ctcattgtag cggggatatt tgggatctag ccaataaacc ttctcactaa tatcattgta
     1861 atgcttatcg tttattgtca tactaactcc tcctgtccgt tagaaaaaat tacttttact
     1921 ctttttatag tgactcctgt cttttgtacc gctctgttct caatgccaaa accaccaagt
     1981 tcatttctct ccttccaaaa actataatca aaataaacag atttcccttc attatttgtc
     2041 attgtcacaa gtgcaccata acttccggta acagggtttt ttttaaattc ttctaccttc
     2101 acttccctga tatccgcaaa ggtatttttc agaactttga ctgactgttt ttctactgcg
     2161 agtaaatctt tctgcctttg tgcttctctt ttgtccatat agactttccc tccgatccct
     2221 aatatgacga ttaataaaat ggctaagctg agttttctta ttttccttga caagaacgtg
     2281 cctcctttct ttttatgtaa gtattgacac cttcattatt taagtacgcg aaagtttgca
     2341 ggaatttttt tatttttgaa atttttctcc aatttgctat ggacgattgg cgctacagcc
     2401 atcgcctgca tgccgtcgaa ggagtttttg atttttagga ttttatacat agtgccgtta
     2461 attttaaaac ttaaattttc ttgcatttca ggtacatagt ctctatgttt aggatctagc
     2521 cagtaaacag catcacttaa ttcactatat tgtttgtcag taatcattaa atactttcct
     2581 catttccatt agagtaagtt actttaactt tattgagcgt atttccttct ttttgctttt
     2641 tttcatcaac tattccgtaa cttcctatct catttctttc tttccaaaaa ctataagaaa
     2701 aataaatcga ttgatcttgt ttattagtca ttagaataac tatccggtat gaacccgtca
     2761 tttcatttcg agcagatttt tcaatcttca cttccttgat atccgcaaag gtatttttca
     2821 gaactttgac ggactgtttt tctactgcga gtaaatcttt ctgtctttgc atttctcttt
     2881 tgtccatgta aaatttccct ccgattccta tagtaagaat ggtcaaaatg atgactatgg
     2941 tctttcgtct tttcttaggc aaatagaagt ctcctttcgg gttatatatg atggattgtt
     3001 tctcctttaa gataagtacg caatcgagcc tggatttttt tggatttttt agagttactt
     3061 atctactttt ctactttctt ttttatcacg ttttcatttt aaagtaattt tgatagtagt
     3121 gaacaaaatg tagcatctac gcacagattg tgtcgtatac gatggacgta aggtttgttt
     3181 ccgccatttt ctcttttttt aaagtcacaa aaaaaaaggg gttatgacaa aattttgtca
     3241 taaccccctt tctcgtataa acgttgttcc agaagttgac cacttttgga acaaccttta
     3301 aatgattgat cttttattat ttagttgatc ctttttcttc gattccataa ggcaagataa
     3361 tcgttttttc ttcgacttct tctttattca tcattttcgt caataggcgc atggataccg
     3421 ctcctaaatc gtataatggt tgtgtgatac ttgtcaaacg aggacgagct acttctgtca
     3481 gtaaagaatt gttgcttgtg atgatttcga attcttctgg tacttttacg ccgcggtcta
     3541 ataaaccatc caagatacca atggctaatt catcgtctga aacatagaca gctgttgcac
     3601 cgctattatg gatccggtca actagagaca gaccatcttt aaagttgtat tgtgcctcaa
     3661 aaatcaatcc ttcgctatat ggtagattgt tttcgcttaa tgcttcttta tagcctttta
     3721 gacggttttg accattgata ggatcaatca gcgcaccgct gacaaaagcg atttttttat
     3781 tgccgctttt tgctaattta tttactgcat cttttgttgc tgacacataa tcaatgttta
     3841 cgctacctac ttgttcatct ggatcaatgg aaccagctag tacaacaggc gtttttgaac
     3901 gtgaaaattc tccacggatt tcgtctgtga tatggtgacc cataaagatc acgccgtcca
     3961 cttgcttagc caataaatta ttcaatacat tgacttcttt ttgatcgttg ccatctgagt
     4021 ttgctaagat gatattgtat ttgtacattg ttgccacatc gtcgatacca cgtgctaaag
     4081 aggcaaagaa catattgctg acatcaggaa tgattacccc aactgttgtt gttttcttac
     4141 ttgccaagcc acgtgcgact gcatttggac gataatccaa gcgatcgata acttccaaga
     4201 ctttttttct tgttgctggt ttgacgtttg gattgccatt tacgacacgt gaaacagtag
     4261 ccatcgaaac atttgcctct ctcgctacat catagatagt gattgtttgc ttttccattt
     4321 gtgttctcct attcatattt tatatgtttt ctgaaaatac gtcttacatt tctaaaatag
     4381 gatatcaaat aatctcaaca attgcaaccg ttttatctct gttttcatga aaatttttac
     4441 ttttttcagt tttaagaacg ttggtctttt aatcaatcag tggtacaatt attgaataca
     4501 aaattaattt agaaagtagg ttgccaaatg aataacgtaa aaatcaatga actacgtcaa
     4561 tggatggcac aacaccaagt agaattggct tatatcagcg accctggcca catcgcttac
     4621 ttttccggat atgaaagtga tcctcacgag cgtgtactag ctctttttat ccctctggaa
     4681 caagatcctt tccttttcac tcctgctctg gaagttgaag atgctcaaaa cagcagctgg
     4741 gcgtacgatg tacgtggcta cttagacagc gaaaatcctt gggagatcat cggaaaagag
     4801 cttcgcgcac gctatgggca acctggtaat gtaggtattg aaaagaatgc tctgactgtc
     4861 gatcgcttcg atgctatctc tgatatcttg acaggtacag attcttacac cgatctaact
     4921 tctattatcc agcgtctgca actgatcaaa acggtagaag aaaaagacaa actgatcgaa
     4981 gctggtaaat gggccgatgt tgcctttgaa attggcttca aaacagtaaa agaaggcgta
     5041 actgaacaag caattatcgc agaaatcgaa taccaattaa aaaaacaagg tgtttcacaa
     5101 atgtctttcg acacattagt ccttgcagga gcaaatgctg caagccctca tggtacacca
     5161 ggaagcacga aggtttcacc aaatgaacta gtgttattcg atcttggcgt tatttggaat
     5221 ggctattgca gtgatgctac tcgtacagtt gcttatcaaa aaccaactga gttccaagaa
     5281 aaaatctata acatcactct tgaagcacag cttgccgcac aagaggcagt tcgcccaggt
     5341 gtgacagcag gtgaacttga tcaaatcgca cgaaacgtga tcaacagcta tggctacggc
     5401 gaatatttca atcaccgttt aggtcacgga atcggaacaa ccgtccacga attcccttct
     5461 cttgtagaag gaaacgactt agtgattgaa gaagggatgt gcttctctct tgaaccagga
     5521 atctatattc ctgaaaaagt aggggtacgt atcgaagatt gtgtgtatgt gacaagtgat
     5581 ggttgtgtgc cattcacgac tacaccaaaa gaattacttg ttttagagta atgaaaataa
     5641 aaaaggtgag agctggtccg ctctcacctt ttttattcta gaaatgagta atcttagtga
     5701 ttggtagtcg aatctgtcat tgtatctttc atttcttttt tagcttcttt tgcatcttct
     5761 tttgccattt tcaacatttc ttttgtgtca tctgcaactg gttttgcttc tgacattcct
     5821 tcattgaccg tctctcctgc tttatctgca gcatctttta catcgatcat gatatcttct
     5881 gaagtatctt tagcaatact acctaattcg tctgctggct ttttcagatc ttctgctgtt
     5941 tttttgaaac gattagataa atcagctgat tgttctttga ggtttaacaa gatatcatct
     6001 gattcacttc ctgcttcctc tgctgattca cttaccttct gcttaacatt gcctgctaga
     6061 tcccctgctt gttgagaaag accttgcgct ttttcttttg caatacctgt taactcagat
     6121 gatttttctt ttgccatatc tgtgtaatct gaagccatgt ccatcaaatc atctgcttga
     6181 gcagccaatt catcccgaag ttctttacca gatttgggtg ctaacaataa tgcggcgatt
     6241 gctgcggctg tacctccaac tactgcacct aataaaaatc cacctttttt agccattctt
     6301 attttcctcc tgtcgtttca gtatgttttt tgtttttaaa aaatttcatc gctgttccac
     6361 cgacttttcc ggcaacagtc gctttagctg tcgtttttcc gattccgccg actttcgtca
     6421 cgatgttttt actagaatga ttaagttccg atacactttc acttaaatca gcaactgctg
     6481 tgaacaatgg atcgatcgtt gcgacttttc cattgatatc caagagcagc tcattacttt
     6541 tgactagcaa gccttctact tgtctagata aaagatctac gtcttgtgtg acgatctcaa
     6601 ttgtcgtatt tgcttctgcg acagtctggt ctactttttc taaagttttg gaaacttgca
     6661 acaatacgcg aacgatgaaa acgacaagta tcgcaaaggc gattgctgcg atcaatcctg
     6721 caatttctcc tcctgacatg atcaaactcc cttcttgtct ctcttttcta taaggatagc
     6781 attttcgtgt tttttaaaca aataaaaacg agcttcaact actttttatt caattagccg
     6841 ctgaagctcg ttagaaaaaa agattatttt ttcttttctt ctttgatttc tgttattcct
     6901 tttggattat gattgatctt gcttccttct gcttctaatg ttttacctaa agacatgatg
     6961 tattcacgca aaggtgcccc tacttctgga tgtgtcagac catattcaat acttgttttc
     7021 ataaaaccaa atttgtcacc aacgtcataa cgtttacctg tgaatttacg ggcaaatacg
     7081 cgttgtgttt tattcaacgt atcgattgcg tcagttagtt ggatctcatt tccagcacct
     7141 ggttgttgtt ctgataatac atcaaagatt tctggagtca ataagtaacg cccaataata
     7201 gctaaatcac ttggtgcttt gcttggatct ggtttttcaa caaagttttt gacattgtat
     7261 aacccatctt ccagtacttc ccctgggttg atgattccgt attttgaagt ttcatcgtga
     7321 ggaacttgca taacggcaat cgtagaagcg tgtgtacgct catagtcatc gatcaattgc
     7381 tgtgtcaatg gtacttcgtc ttccattata tcatcgccta gcataacgac aaatggttca
     7441 tttccaataa aggctcttgc ttgaagaacg gcatgtccta aaccctttgg atgagattga
     7501 cggatgaaat gcaagttgac atctgtcgtt tcttctacta atttcaataa ttcggtcttt
     7561 cctttttcac ttaggtttgt ttctaattca atatttgaat caaagtgatc ttcgattggt
     7621 cgtttagctt ttcctgtaac gatcaagatg tcttcgatac ctgatttcaa cgcttcttca
     7681 acgatgaact ggattgttgg tttatcaaca attggcaaca tttcctttgc catagctttt
     7741 gttgctggta aaaatcttgt tcctaatcct gctgctggaa taactgcttt tttaactttc
     7801 atatacattc gctccttcaa aaaattgtta atgagaattc attttctttt tttccatcac
     7861 gtaacatgat ttcttttgcg gctgttttga tttctttttg ttcatacagc acttcataga
     7921 ttgtttgagt gatgggcatt tcgatttcca tcatttgggc tagttccatc gctgcttggg
     7981 tagtattcac accttcaacg accattccca tattggctag cacctcttct aggggctgcc
     8041 cttgtcctaa caagttgcct gcgcgccagt tcctagaatg aacacttgtg caagtaacga
     8101 ctagatcgcc tattccgctt aatccgataa aggtcagcgg gttggcaccc attgctacac
     8161 ctaaacggct gatctcagct aatccgcgcg tcatgattgc tgcctttgca ttatcgccaa
     8221 accctaagcc gtgaatagcg ccagcaccaa tagcaataat atttttcaaa gctgccccca
     8281 tctctacacc gatcacatca ggattggtat agatccgaaa ataattattc atgaacagct
     8341 cttgtacata ttttgctgct tttgcatctg tacttgcagc tgtgatcgta gtgatgtcat
     8401 gttttgctac ttcttctgca tggcttggtc cagaaagaac aacaacagct tttctttttt
     8461 ctgctgggat ttcctctaac aagatgtcag agatcctttt atgtgttccc tgttctaatc
     8521 ctttacttgc atggatgatc actggctttg tattcaattt aggcactagt tcttttgcaa
     8581 cagaacgtat tgctttcgtt ggaatgacga atagcaccgc atctgcgtct tttactgcat
     8641 cttccatatt ggtgaatgct tggatggaat cgggtagata atgatctggt aaataacgtt
     8701 gattcgtgtg ttgggtgttg atttcttcta cttgtgaagc atgatgtccc caaatccgaa
     8761 cgtcatgtcc gttttcggcg aggacttgtg ccaaagcagt accccaagag cctggtccta
     8821 aaaccgctac tctataattc attttaccac tcctgaaatt tttttcattt ttaacgctct
     8881 aaaaatcaga taatttgttg attttttcct aatgagcggt cataatccgg taaatttcct
     8941 ttttttctgc gccaaataaa tagaatcaat gtaccgacaa ataacacagc agataataat
     9001 tgagaaatac ggatcacacc taaaaagtaa agactgtctg tacgtaatcc ttcaataaag
     9061 aaacgcccga aactatacca cattagataa cataaaagga cttcgccttt tttcaaaagg
     9121 tgtggtttct ttcgtaaaaa caaaagcaaa ataaatccaa tcaaactcca aagtgattca
     9181 taaagaaatg ttggttgacg atagatccct tcgatataca tattatcaat gatgaattgc
     9241 ggtaaatgca gactttctaa gaagcttcgc gtcgtgtctg gtccatatgc ttcgtggttc
     9301 atgaaatttc cccaacgacc gattgcctgt gctaataata cacttggtgc agcgatatct
     9361 aaaaaagtcc acaaagaaat aaaatgatat ttagtaaaaa aatacagcgt gatcgcgcct
     9421 gcaatcagtc cgccataaat agctaagcct cctgaacgga tattgaatat ttggattgga
     9481 tcagctatat actggggaag atcaaataaa acataatata agcgagcgcc aataatggag
     9541 ataggcaatc cccacaacat aaaatctgtc acatcatctg cttttaatcc tacccgaaca
     9601 gcctctctgc tgctaagcca catagcgata atgatacctg atacgatgat caatgcatac
     9661 cagtagatag gaatccctac aagctcaaaa gcaactcgat tgatttgtgc taacatattg
     9721 tcactccttg ctagtcgttg cctgagtttt cttcaatcaa gcgacttaat ctttcttcaa
     9781 atgttttcgt tgcgtcaaag cccattgtac gtgcacggaa attcatcgcg gctacttcaa
     9841 tgatgattgc aacgtttcgt cctgttttga cagggatttt tatttgtgga atgcccactt
     9901 ccgcgatttc aacggtcg
//
LOCUS       DAFBYI010000093         9873 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFBYI010000093 DAFBYI010000000
VERSION     DAFBYI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.093"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(22..267)
                     /locus_tag="LLD04_002132"
     CDS             complement(22..267)
                     /locus_tag="LLD04_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK5649049.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="LLD04_002133"
     CDS             complement(381..959)
                     /locus_tag="LLD04_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5649050.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="LLD04_002134"
     CDS             complement(1036..1566)
                     /locus_tag="LLD04_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK5649051.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="LLD04_002135"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="LLD04_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK5649052.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="LLD04_002136"
     CDS             complement(2423..2818)
                     /locus_tag="LLD04_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK5649053.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="LLD04_002137"
     CDS             complement(2815..4227)
                     /locus_tag="LLD04_002137"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK5649054.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="LLD04_002138"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="LLD04_002138"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK5649055.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /locus_tag="LLD04_002139"
     CDS             complement(5082..6542)
                     /locus_tag="LLD04_002139"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK5649056.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="LLD04_002140"
     CDS             complement(6712..7869)
                     /locus_tag="LLD04_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK5649057.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="LLD04_002141"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="LLD04_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK5649058.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="LLD04_002142"
     CDS             complement(9336..9863)
                     /locus_tag="LLD04_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK5649059.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DAFBYI010000094         9663 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFBYI010000094 DAFBYI010000000
VERSION     DAFBYI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.094"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            269..1102
                     /locus_tag="LLD04_002143"
     CDS             269..1102
                     /locus_tag="LLD04_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK5649060.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1204..2904)
                     /locus_tag="LLD04_002144"
     CDS             complement(1204..2904)
                     /locus_tag="LLD04_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649061.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            3411..4571
                     /locus_tag="LLD04_002145"
     CDS             3411..4571
                     /locus_tag="LLD04_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5649062.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            4587..5030
                     /locus_tag="LLD04_002146"
     CDS             4587..5030
                     /locus_tag="LLD04_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649063.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5513..6043)
                     /locus_tag="LLD04_002147"
     CDS             complement(5513..6043)
                     /locus_tag="LLD04_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK5649064.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            6148..6699
                     /locus_tag="LLD04_002148"
     CDS             6148..6699
                     /locus_tag="LLD04_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5649065.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(6852..7325)
                     /locus_tag="LLD04_002149"
     CDS             complement(6852..7325)
                     /locus_tag="LLD04_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5649066.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            7463..8935
                     /locus_tag="LLD04_002150"
     CDS             7463..8935
                     /locus_tag="LLD04_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5649067.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            8932..9642
                     /locus_tag="LLD04_002151"
     CDS             8932..9642
                     /locus_tag="LLD04_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK5649068.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         3218 a         1678 c         1888 g         2879 t
ORIGIN      
        1 ataagaacag ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg
       61 agaagcagca gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa
      121 gtgataaaaa cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt
      181 tagcaggaga acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg
      241 tttcgaaatg aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg
      301 gcttgagcca gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga
      361 ttatgaagtg attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc
      421 tgcgaagcta atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt
      481 tgcctaccgt ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga
      541 aatgagccac atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac
      601 attggaaagt caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg
      661 caagatcttg attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca
      721 cttttcttgg tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa
      781 ctactttttg gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga
      841 ctctcaaggt tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt
      901 cgcgaatgaa aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa
      961 tacagaaaaa ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata
     1021 tcaacggatt tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat
     1081 cgaattagat gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata
     1141 aaactctcca aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt
     1201 tgcctaagca cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca
     1261 gccaatcaaa gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt
     1321 gagctcgttg attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata
     1381 atcagtcaat gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat
     1441 atgaacagtt ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt
     1501 ttttataaaa gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc
     1561 ttttgtctcc ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct
     1621 ggtagacata tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata
     1681 agtatgtcga ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg
     1741 gatcgtctgt gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg
     1801 ttcagaccac tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac
     1861 gaaaaatcga aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt
     1921 cgtccatgga atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat
     1981 tttttggaat aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat
     2041 caatatagga aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt
     2101 ttgactcatt tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc
     2161 taatcctgcc caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg
     2221 aagcagtgct tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata
     2281 catcaaagct aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac
     2341 agcgactttg ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca
     2401 taaaggataa aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat
     2461 agaatacgta ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac
     2521 atatctcagt aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag
     2581 aatctgataa tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa
     2641 aaatgggccg taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg
     2701 aaaataatgg aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg
     2761 gaatggggca tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc
     2821 caaagcaatg aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt
     2881 gcaaacttta gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa
     2941 ttcatgaaaa tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag
     3001 gatgcgacat aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg
     3061 aaacaaagta gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt
     3121 aataagctca ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact
     3181 caaagcataa cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg
     3241 cggtaataag cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt
     3301 acttgatgca ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg
     3361 aacccatata ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga
     3421 gtaagcaaaa acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg
     3481 tagggttact gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc
     3541 aaaaacgtat cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga
     3601 aaatggagga gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt
     3661 tcaatgaaaa gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg
     3721 gaagtatcaa tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta
     3781 ggatccttga aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt
     3841 cgacgcattc agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat
     3901 tagatcgttt agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt
     3961 atcaagttaa aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag
     4021 aaggagaaga cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt
     4081 tgctagtgac aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag
     4141 gaaatcagga aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag
     4201 tcatactgtt agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc
     4261 ttgatttttc atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca
     4321 ttttgaaaca taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg
     4381 accatcatgg gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag
     4441 ctgatgatcc taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat
     4501 acttttttga ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa
     4561 tcaatgaata agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag
     4621 aactggaatc tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc
     4681 catcaactta ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga
     4741 tttacttagc agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta
     4801 tcgcaggaat ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga
     4861 aaaaaataaa agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag
     4921 ctattcattt ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag
     4981 taaatgagga ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat
     5041 aacgaaataa tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac
     5101 taatttaact gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct
     5161 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc
     5221 tctaatactc aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc
     5281 tgcttctcgg taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg
     5341 ttcttattac tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca
     5401 gctgaccctc ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct
     5461 ttctctaata ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta
     5521 ctcgtttata attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt
     5581 atggttcttc gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg
     5641 tagcaaattc aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca
     5701 tttctgcgat atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt
     5761 tagggaaaaa gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt
     5821 ctgtccctgt tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat
     5881 cagcttcttg gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa
     5941 cagtatggtc atttttggca taccattcat attcccaacc attgtcatac gtatagataa
     6001 agtgagttcc aataaaatct tccaattctt taaattcttt catgtttaag catctcctta
     6061 taaattaaaa catgtaataa actacatgtt ttcgattact catttatgat agaatggatt
     6121 taaagatttg tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg
     6181 ttagggttat taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat
     6241 gaaataggag aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg
     6301 gaagagcaag gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa
     6361 gtttatgaat taacagaaga agggaaggaa caattggcag agtggattca tacacccacc
     6421 aatgaactac cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg
     6481 aatgatccgg cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga
     6541 cttcatctat tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat
     6601 tacggacact acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg
     6661 ctaacagatg tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg
     6721 gagaattcct ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa
     6781 caggcctttt ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag
     6841 attagtggcg tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga
     6901 aggagcttgt aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc
     6961 tcttctttga agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct
     7021 ttgattgctt ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc
     7081 ttttctgcag cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact
     7141 tttatgactg taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga
     7201 atgaatccct tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt
     7261 aattcactga tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca
     7321 accatttttg tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat
     7381 gtgtcaagta ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa
     7441 aaattcagaa agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc
     7501 atataatcgg acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa
     7561 tcagacctta ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc
     7621 tgatgtgcaa tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac
     7681 agcatggctg atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt
     7741 tttgatcgga acgattactt gctttacagc acctaatttt agtacattac taatcggtcg
     7801 tttgatccaa gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc
     7861 tattttcccg ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact
     7921 tgctcctgct ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga
     7981 cttgtttggt atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat
     8041 gaaaagtgtt attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac
     8101 gattggattc ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag
     8161 cttgaaagta attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg
     8221 tcaattacat ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac
     8281 gattgcagct gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt
     8341 accattgtat attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt
     8401 gccgggtgca ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata
     8461 cggcgcgaaa cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt
     8521 tgctttcttg acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat
     8581 gtttgggatc tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa
     8641 taacttgatc agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat
     8701 tggtacagct attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa
     8761 atctgtattg gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc
     8821 aggttaccat gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc
     8881 ctttttattg aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt
     8941 ggagttttga ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca
     9001 tggcaaacct ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc
     9061 gtcgctaatt tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa
     9121 attgtttcca gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat
     9181 ggaaaagaaa aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact
     9241 ggtacggata acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg
     9301 aaaactactt attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat
     9361 aacaatcacg aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa
     9421 ttaagtacgg accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg
     9481 gaaagtgaag caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca
     9541 aaaatgagca aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa
     9601 caagcattcg caaaattagt gaaagaagca aaaggcgaat aatattttga acaccgttta
     9661 agg
//
LOCUS       DAFBYI010000095         9510 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFBYI010000095 DAFBYI010000000
VERSION     DAFBYI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.095"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            6..2378
                     /locus_tag="LLD04_002152"
     CDS             6..2378
                     /locus_tag="LLD04_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649069.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3908
                     /locus_tag="LLD04_002153"
     CDS             2439..3908
                     /locus_tag="LLD04_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK5649070.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3919..5928
                     /gene="topB"
                     /locus_tag="LLD04_002154"
     CDS             3919..5928
                     /gene="topB"
                     /locus_tag="LLD04_002154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5649071.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6044..6193
                     /locus_tag="LLD04_002155"
     CDS             6044..6193
                     /locus_tag="LLD04_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649072.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6274..6870
                     /locus_tag="LLD04_002156"
     CDS             6274..6870
                     /locus_tag="LLD04_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK5649073.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6883..7782
                     /locus_tag="LLD04_002157"
     CDS             6883..7782
                     /locus_tag="LLD04_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK5649074.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7785..7916
                     /gene="ssb"
                     /locus_tag="LLD04_002158"
     CDS             7785..7916
                     /gene="ssb"
                     /locus_tag="LLD04_002158"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5649075.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7938..8270
                     /gene="ssb"
                     /locus_tag="LLD04_002159"
     CDS             7938..8270
                     /gene="ssb"
                     /locus_tag="LLD04_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5649076.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8315..8548
                     /locus_tag="LLD04_002160"
     CDS             8315..8548
                     /locus_tag="LLD04_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649077.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8707..8829)
                     /locus_tag="LLD04_002161"
     CDS             complement(8707..8829)
                     /locus_tag="LLD04_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBK5649078.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9019..9243
                     /locus_tag="LLD04_002162"
     CDS             9019..9243
                     /locus_tag="LLD04_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649079.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT         3524 a         1623 c         2027 g         2336 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa gaagggttga
     2881 ttgctgaact aaagccgaaa catgaacggc aaaatttttt agttggagac gaccgtttag
     2941 atcatgcggt tgctttttta tggaaagatc cacagacagg agagacagta ggggcaagtt
     3001 atcaaggaac gatcgtcgat tttaatcgtt tcggtaaacg gggaacgtat aaacacattg
     3061 acaagaatcc tacgccaaat cacggattta atttaaaaat aggcgatccg aagcatttaa
     3121 aattctttga aagtagtatt gatctgttaa gttacgctgc attgaatcgg gagaaactac
     3181 aagatgcctg gttagtttcg atggacgggt taaaacatca tgtaatcagt cattacgttg
     3241 aagagtcgat ttctgaatta agacgaaaac aaacatttcc tcaatcgatt gaaatatgtg
     3301 tggacaatga tcgtgcagga catatttttt atgaaaaaga acagatgaag ggaatcgttg
     3361 atccttttac caataagaaa atccgttgtg agcgaggaat tccaaatgat tggcaagtac
     3421 ctaaagaata caaagctact tatgaagcag tcgcaaaaga aatgagtgtt gaacctgaag
     3481 caattatggc catacacaaa actgaaacta atttacagct gacaaatcaa ttggtgtctg
     3541 ctcatgatgt tcaatctacg tttggaaaga tgttggcgaa gggcgaacca gtagaaacaa
     3601 ttgatttgaa agaagcatgt accacggtcg cgaaggaact gaaagtatgt gaacgcgcag
     3661 atggaacata caactttgat cgtttttata gtagaaaagc caacataaaa gacgttaatg
     3721 caggaatcct tctttcctat aaagcagaac aatactacaa aggctataaa aagcatgaac
     3781 atgaatttgt acctgaagtg aaaaaggatt ggaatgatca attgaagcat gagatccagc
     3841 aacaagaaat cagaaagcaa aaacgtgcga tgttgtttca acaaggcaga caacaagaaa
     3901 gggagtgaag tcgtcactat gaaaaccgtc attttagcag aaaaaccgtc acaagcgaag
     3961 gcttatgccg atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat
     4021 cgattgttta caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat
     4081 tcgcccgata tgtacgatga aaaatggaaa caatgggcat tagaacattt gcctattttc
     4141 cccaatcaat accactatca tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa
     4201 cgacagctgc agtcagctga tacgattgtg atcgcgactg atagcgatcg tgaaggggag
     4261 ctgattgcct ggtcaatcat tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga
     4321 ctttggatca actccttaga aaaagaagcc atttatcagg gattccaaca gttacgagac
     4381 gcagaagaga cctatccaaa atttgaagaa gtacaagcac gtcagatcgc cgattggtta
     4441 attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga
     4501 agtttttctt taggtagagt ccaaacccca acactctata tgatttatca attgcaggaa
     4561 aagattagaa acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa
     4621 aatggcgcat ttgatgcgaa gcttgatcca aatgaaaccc aagcgacaca agaagcattt
     4681 gaagcctatt taaaagaaaa aggtgttcaa ttaggaaaac agccaggtac gatccttcaa
     4741 gtcgaaacag agaaaaaaag tgcggctagc ccacgtcttt tttctttatc gagccttcag
     4801 tctaaaatga atcaactcat gaaagctagt gccaaagata ctttggaagc catgcaaggg
     4861 ttgtatgaag gaaaatatct aagctatccg cgaacggata ccccttatat tacggaaggg
     4921 gaatatgcgt atctgttgga tcacttagac gaatacaagc attttttaaa agccgaagcg
     4981 attccgactc ctattcatac accgaatagt cggtatgtga ataataagaa agtccaggaa
     5041 cattacgcga tcattcctac aaaaacggtt atgacagcag cggcttttga acaactaagt
     5101 ccactgcagc aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa
     5161 aagtatacct atgaagaaac aacgatcttg acgcaagttc agcagcttca gttaaaggca
     5221 atcgaaaagg tgccgctaga tctaggttgg aagaagttat ttggaaaaga gagcgacgga
     5281 aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggtg
     5341 gatctacaag ttcttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg
     5401 atcactgcaa tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg
     5461 aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa
     5521 caaaaggaat atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg
     5581 ctttgccaag ccgtggaaaa agagccgctg cttacgagcg ccgaaatgac cgccaaatgg
     5641 gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat
     5701 atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat
     5761 tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat
     5821 ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct
     5881 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
     5941 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
     6001 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
     6061 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
     6121 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
     6181 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
     6241 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
     6301 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
     6361 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
     6421 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
     6481 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
     6541 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
     6601 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
     6661 cgctatggga atcctattat agcttatgat ccagaaaaaa ccttgtgtga catttggaat
     6721 ccctggtata acgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta catggagtcg
     6781 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttgcctac agataaaacg
     6841 atgcgctctt atattatggc tttaaattaa aggagaatac acatgacacc tacgcaatta
     6901 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     6961 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     7021 gtgttaaaag gcggcttctt aattggctca aagtatggca ttgaaaatcg aacaactaaa
     7081 gacattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattgac aacggttcta
     7141 aacgatattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     7201 gaaacgaggg aagctgatta ttatccaggg tttagcttac gtgtactggc acatttggaa
     7261 aacatgcggc cagattttaa ggtagatgtt actacaggtg actcgatcta tcctgcgacg
     7321 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     7381 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     7441 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     7501 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     7561 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     7621 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     7681 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     7741 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     7801 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     7861 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     7921 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     7981 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     8041 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     8101 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     8161 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     8221 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     8281 aaggagtaag catggcaaaa aaattagtct gacgatggat ctattgatac ccgtaattgc
     8341 atttatagac gggatttctt tgggcgtcac tgaaaatcct ttggtaaata gaattgcgaa
     8401 aggatcacta tttcttgtat tcgtgtatct tgtctgttct tatcttttaa aaagagcagg
     8461 aaaggataat tccttttggc gattgactaa agtcacagta gcagcgacag ctgttatact
     8521 tacggctatt ctaccgcttt atatgtagag ttaacgggaa atcgttgaac ttcttgcgaa
     8581 atattgctat aatgaacatg aaaaagagga gttgcgctaa caacccctca ttaacagcac
     8641 cgattaaaga acggtggcag aatattagtt atttggaaat aaccgctgac ctgctaaagt
     8701 agacggttat ttctttttgt catttttaag catatttaca atcaaactaa tcaaagcaat
     8761 ggtaaacata ccaaaaccaa gaatggtttg aattgtctca tatgcggaca aaaggcttac
     8821 tcctttcata gattttagca cttacattca taagcaccac cacctttcca gaagtcgcca
     8881 ccgtcttcaa actttactgt cttgactatt atagcatatc taaaatcgaa taaaaattct
     8941 caattttagc tgtttttgct ataattttag cgaggtgatc gtgatggaac aatatattgg
     9001 tttactcgat aaattagggt gatcaagaca cagccattgc ttgtgcgatt tacattacgc
     9061 acgagtcatg aaccaatcaa ttgtgtagtg gccaatatag aaatagcgga taaattgctg
     9121 attttggaag aaggcaagta tactatttca gtgacaggtc acttcaataa acgaaagcaa
     9181 ctagtcattg aatcaatgca aattagaaac cctgatcatt ttacgcgatc gatgagcata
     9241 taaaagaaga gctgcagtcc taaatgagga ttgtagctct ttttgtttta catagaaaca
     9301 gtaaaaatga aaaggcctgt ttaggaaggc acaagcgcct tctcaattga aagacctttc
     9361 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctac gcgttcacta
     9421 agataaaggg tcttgcatag caagacaaaa tagaaaaaat ttcctctcac caaaaggttc
     9481 gctcctgttc gttatctctt tattgatttg
//
LOCUS       DAFBYI010000096         9500 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFBYI010000096 DAFBYI010000000
VERSION     DAFBYI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.096"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            1..267
                     /locus_tag="LLD04_002163"
                     /pseudo
     CDS             1..267
                     /locus_tag="LLD04_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            806..1006
                     /locus_tag="LLD04_002164"
                     /pseudo
     CDS             806..1006
                     /locus_tag="LLD04_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="LLD04_002165"
     CDS             1493..1672
                     /locus_tag="LLD04_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649080.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="LLD04_002166"
     CDS             complement(1718..2323)
                     /locus_tag="LLD04_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649081.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="LLD04_002167"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="LLD04_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3005..3232
                     /locus_tag="LLD04_002168"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="LLD04_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="LLD04_002169"
     CDS             3358..4329
                     /locus_tag="LLD04_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5649082.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="LLD04_002170"
     CDS             4469..5782
                     /locus_tag="LLD04_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK5649083.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="LLD04_002171"
     CDS             5825..6691
                     /locus_tag="LLD04_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5649084.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="LLD04_002172"
     CDS             6688..7536
                     /locus_tag="LLD04_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5649085.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="LLD04_002173"
     CDS             7550..9022
                     /locus_tag="LLD04_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK5649086.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9500
                     /locus_tag="LLD04_002174"
     CDS             9039..>9500
                     /locus_tag="LLD04_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5649087.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEM"
BASE COUNT         3008 a         1598 c         1958 g         2936 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa attggcatgg caaaattttg
     9301 atttcatcgg taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca
     9361 atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt
     9421 actatacgat cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag
     9481 ggtttagtca tccagaaatg
//
LOCUS       DAFBYI010000097         8993 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFBYI010000097 DAFBYI010000000
VERSION     DAFBYI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.097"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            54..506
                     /locus_tag="LLD04_002175"
     CDS             54..506
                     /locus_tag="LLD04_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649088.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="LLD04_002176"
     CDS             520..1209
                     /locus_tag="LLD04_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649089.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="LLD04_002177"
     CDS             1334..3685
                     /locus_tag="LLD04_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK5649090.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="LLD04_002178"
     CDS             3765..4160
                     /locus_tag="LLD04_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649091.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="LLD04_002179"
     CDS             4485..5102
                     /locus_tag="LLD04_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649092.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="LLD04_002180"
     CDS             5143..5442
                     /locus_tag="LLD04_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK5649093.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="LLD04_002181"
     CDS             5476..5643
                     /locus_tag="LLD04_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5649094.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="LLD04_002182"
     CDS             complement(5688..6251)
                     /locus_tag="LLD04_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649095.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6279..6959)
                     /locus_tag="LLD04_002183"
                     /pseudo
     CDS             complement(6279..6959)
                     /locus_tag="LLD04_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(7034..7393)
                     /locus_tag="LLD04_002184"
                     /pseudo
     CDS             complement(7034..7393)
                     /locus_tag="LLD04_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            complement(7981..8183)
                     /locus_tag="LLD04_002185"
                     /pseudo
     CDS             complement(7981..8183)
                     /locus_tag="LLD04_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8383..>8993)
                     /locus_tag="LLD04_002186"
     CDS             complement(8383..>8993)
                     /locus_tag="LLD04_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5649096.1"
                     /translation="LASSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKAVVPKEIETWWRLELQLRR
                     GKATDWHKMVHESLDSFASPHFLPIDTKHTDRIMIAGLMSDHSLWSGLTRPTKYKYRN
                     LLKQESQNDELTNHLRETFSESADNLKQELDTWLLGLDVTEK"
BASE COUNT         2953 a         1458 c         1678 g         2904 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 ttttaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgcgccct ctctgaactc caaatatctt aggctgttat tcccattaat
     6301 accttgattt cagtagacac cgaaaagccg aagagcgttc catttcttcg gttcttttta
     6361 tatattcctc gaatggtctc catgccctta atcgtggaag aggctgtacg gagactttga
     6421 taaaatttat ttcgtcgttt aataggtcga tggtcttgtt ctattaaatt gttaagatac
     6481 ttcacagttc ggtgctctgt cttagtatat aaacccacac tctgtaactt tctaaaggcg
     6541 gagccaagag aaggtgcttt atcggtcaca attgctttcg gctcaccaaa ctgtttatgg
     6601 agtcgtttta agaaagcata ggctgcttgc gtatcccgtt tctttcgtaa ccagatatct
     6661 aaggttaagc cgtccgcatc aattgcacga taaagataat gccaacgtcc cttaattttg
     6721 atataggttt cgtccatttt ccatgaatag aaggattgtc tatttttctt cttccaaaga
     6781 taatagagga ctttgctgta ctcttgtacc caacgataaa tcgtagtatg acaaacattt
     6841 attccacgat catataacaa ttcctgaact taacgatagc ttagattgta acgcaggtag
     6901 taaccaacag cgacaataat gacgtctttt ttgaattgtt tgcctttaaa atgattcatt
     6961 actctgtcct ctctgtcttt tttctcaatt ttacactaaa atagattttt tggaaaactt
     7021 tgcaacagaa ccctaaaaac tgattaattt tattcggatt aaagtcaatt cgcccttgcc
     7081 cttcttgata tttcaaaatt tcaatgtaag caatattctc atcatgctta tccacaattt
     7141 gccaaccatt tagaccaaca gatttaacat aaccattatt ttcattcagt cgcattaact
     7201 gttcaaagtc taaaatcaat acatcatttt gagtatgata ataaatattt tctttcagtt
     7261 ttccaacaat ggtaattcgg tcaattgacc accgtatttt caagggggtg taaaaagttt
     7321 ttacatttaa ccgcctgtta gatagggctg ttaaattttc ccactcattt acaatcgtgc
     7381 catttttact caaatttcgt tcctcctcaa agaaaaaaga gattcttgga aagaatttta
     7441 aaagttcacg taacccttaa aattcattaa caagaatctc tagtcttgaa gaaacctaaa
     7501 aatattcgta taatgattat gagttacgga gctagtgcga tagtttccgt aattagtcga
     7561 aaggtgcttc caacaccaat cggctatttt tttgctcttt tttcgatttt tgataattat
     7621 aacaccagaa aacaaaaact gacaaatact cccgagtata aaagacacaa gaccttgggg
     7681 aatgtcattc cccaaacccc ttgagcctcc tcctgccgaa atggaagggc gcacccgctg
     7741 tttatttcat tcagctatcg gctttcacaa tctaacacga ccgttccgct accagtcgcc
     7801 ttacaggctc cattctcgct cctctaacgt gttttaacga ttgtgccgat aactaaacga
     7861 aataaacgct aaaaccgctc agaacgcaaa atatgccgtt ttagagctat tgtagaaaat
     7921 gtagtttgaa atgcaatttt cttctttcag ctttttattt cccacttgta aatctgctaa
     7981 ttattttcta ccagacaaac cattgatttt ttggctatca gttaactcac gattattatg
     8041 agaaatgctt gtattctttg ttcccttttt gaacgaaata ctgaacccca agttaaaacc
     8101 ctccttcggc tgtcgctaat aaaaagagtg taaacctttc tgttactctt tgtcaaagag
     8161 taacattttc ctaattgtga catggctgtc acaattccaa acgctctccg agggttttcg
     8221 ctgtcacaag tgacaacgaa aaaaggagcc gaaagcgtgt cacgctctca actcctttta
     8281 cgctttattt tgcttcatgc aaaatattcg cttatcgtga atttacgtca ctaaacattg
     8341 acgcaaatcc actctcactc atctgacttt tcttattcgg tatcacttct ctgtcacgtc
     8401 caaccccaaa agccacgtgt ctaattcttg tttcaaattg tctgccgatt ccgaaaatgt
     8461 ttctcgtaaa tgattcgtca attcatcatt ctgactctct tgttttaaca aatttcgata
     8521 tttgtattta gttggtctag ttaaccccga ccataaagaa tggtctgaca ttaaaccagc
     8581 aatcataatt ctatctgtat gttttgtatc tattggtaaa aagtgtggac tagcaaaact
     8641 atccaaactt tcatgaacca ttttgtgcca atcagttgct tttcctcggc gcaactgtaa
     8701 ttctaaacgc caccaagtct cgatttcttt tggcacgact gctttttttc gttcctgttc
     8761 cagcttttta ttgtacatac gaacttgacg ttcactagca cgactgcccc aataagcagt
     8821 ttctaacttg ccagaccgac cataaattgg tttaaaggat acaggatcaa ccacccgata
     8881 ttgggtaatg aaatcatctg gtacgtcaat tatatcgcaa gcaatatctg cacgtgaaaa
     8941 atgcggctcc aaaaataaat catggataaa attcttcatc gagctagcta aaa
//
LOCUS       DAFBYI010000098         8928 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFBYI010000098 DAFBYI010000000
VERSION     DAFBYI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.098"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            170..514
                     /locus_tag="LLD04_002187"
     CDS             170..514
                     /locus_tag="LLD04_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK5649097.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LLD04_002188"
                     /pseudo
     CDS             515..934
                     /locus_tag="LLD04_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="LLD04_002189"
     CDS             complement(1026..1376)
                     /locus_tag="LLD04_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649098.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LLD04_002190"
     CDS             complement(1342..2673)
                     /locus_tag="LLD04_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK5649099.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LLD04_002191"
     CDS             2929..3495
                     /locus_tag="LLD04_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5649100.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LLD04_002192"
     tRNA            3640..3727
                     /locus_tag="LLD04_002192"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            complement(3779..4984)
                     /locus_tag="LLD04_002193"
     CDS             complement(3779..4984)
                     /locus_tag="LLD04_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5649101.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLFFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5122..6009)
                     /locus_tag="LLD04_002194"
     CDS             complement(5122..6009)
                     /locus_tag="LLD04_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5649102.1"
                     /translation="MKKMIGSLLVLASLLLFTACGKKVTTDDLKANDWLIEATKDDEP
                     NMIASFSDHIVSLSVDTSKMKSTASDEWDAMGEEFAKSLVDQMNYKFEYTLEGNVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKSNKSDDKEKLVLKPYKKEKTKDSRTTTSSTS
                     SNEQSSSTTTESTIASSSTVEQAAIAQNTPPETSSTYSAESVENYAEATPSSSVEQVS
                     STPSSTEQSVPQDYVPANQAQPTAPSSSETETLTLQPGEGPKQIAERAGITVDQLFAL
                     NGMDPNNYMLYPGQELRVK"
     gene            complement(6096..6296)
                     /locus_tag="LLD04_002195"
     CDS             complement(6096..6296)
                     /locus_tag="LLD04_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649103.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6310..6723)
                     /locus_tag="LLD04_002196"
     CDS             complement(6310..6723)
                     /locus_tag="LLD04_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5649104.1"
                     /translation="MDNIEDLLKENGIDVEITNIESEGFYLPKLRTIFINQNLDELEQ
                     KKVLLHESRHALSHNELISLYSKTVFHSKMEDEANRFMIEVLLQDYMNLFALSVDQIN
                     YMKFMDYYGIGYDCEEYIKKLLVNYVTSSMYLNVI"
     gene            complement(6735..7073)
                     /locus_tag="LLD04_002197"
     CDS             complement(6735..7073)
                     /locus_tag="LLD04_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5649105.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGRTDNSEDSDSQLPPTDLDDALDGAMTWNGKPLTDQDKEAV
                     RIFLQGRKSE"
     gene            7367..7558
                     /locus_tag="LLD04_002198"
     CDS             7367..7558
                     /locus_tag="LLD04_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649106.1"
                     /translation="MSNIDNGREAIKGFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            7569..7835
                     /locus_tag="LLD04_002199"
     CDS             7569..7835
                     /locus_tag="LLD04_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649107.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            7848..8027
                     /locus_tag="LLD04_002200"
     CDS             7848..8027
                     /locus_tag="LLD04_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649108.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8070..8540)
                     /locus_tag="LLD04_002201"
     CDS             complement(8070..8540)
                     /locus_tag="LLD04_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649109.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            8627..>8928
                     /locus_tag="LLD04_002202"
     CDS             8627..>8928
                     /locus_tag="LLD04_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBK5649110.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDV"
BASE COUNT         2958 a         1478 c         1628 g         2864 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atatcaacga ttagacacga tttcgatcgt gtcttttttt gttgtaattt
     3781 agcctaaaat gttttttgac tccccttttg actccccctg agtactattc ttcataaatg
     3841 ctacaaaatt attgatgact tttttgtttt gagtttcagt tacatgagtg taaatatttg
     3901 aagtagtttg aatgtctgca tgccctaaac gttcctgaac atcttttaaa gaagctcctg
     3961 attcaaaaag gagagaggca tgagtatgtc gaaaaccatg agtagtgatt cttttagtta
     4021 attctggatg atgcttatat atgacattaa gccacgaacg tgaaactgta gggttataga
     4081 gaagaccatc atggtgaaaa aataagtttt cgctttggat tgtaacgatc gttttgtttt
     4141 ttgtataata ctttctcaat aaactgatca aatcattgtc cagataaatt tttctttttc
     4201 cagattttga tttaggagta tttacgatca atctcccttg ataacctctt gttacagttt
     4261 tattaatatt gagtgttttg ctcttaaaat caatatccga ccaggtcagt gcaaaagctt
     4321 ctccttttct tatacctgta aaagccaata gggaaaagaa taaatatctt tcatcatcat
     4381 cgtttcttat tgcttctaaa aattcgatta attcttcttt agtataaaat tcgatatttt
     4441 tatcttcgat atctagagct tctccacgtg gaatagaaac taatttcata ggattgctag
     4501 ttataatttg taaagaagca gcgtaatcaa aaacattaga tgtgtaattt tttattttct
     4561 taaaaatttt aggatggcta tcggaccaag tgtttactgc ttcttgacag aaaaaaacgt
     4621 ctattttgtc aatgaactta tctccaaaca cttttaagat gtgtgtgtca aaaatttgtt
     4681 tggtggagga ccaagtgctt tctttaactg tctttttata attttcaaac cataagccgt
     4741 aaacatattc gaatttcttg ctcttttgag ctgtagaagc ttgcagtcct ttttcctgta
     4801 tatctgcctc caatcttttt aaagctctct cagcggctaa tggcgtacta aaacctcgtc
     4861 tcgttgtttt tctttttttt cctgttagag gatcaactcc taagtacaga ctaaactgat
     4921 atttttcttc gccttttttt gttaagtgct ttttaattcg tttatcaatt tctttttttg
     4981 ccatctttat ctttcctttc gtacgtttgt tcggttgtac agcggatttc gagatggtaa
     5041 aatagggtac aacaaataga cctactttac cgtagctctt tgcacatttg cgttcttggt
     5101 cgggcggcaa atgtgctttt tttatttcac tcttagttct tgtcctggat aaagcatgta
     5161 gttgtttgga tccataccgt ttaatgcaaa cagttgatct accgtaattc cagctcgttc
     5221 agctatttgt ttgggccctt caccaggttg caaagtcaag gtttcggttt cagatgaaga
     5281 aggtgccgtt ggttgagctt gattagctgg tacataatct tgtggtactg attgctccgt
     5341 tgaactggga gtggatgaaa cttgttcaac acttgaacta ggagtagctt ctgcatagtt
     5401 ttctacactt tcagcagagt aagtactact agtttcagga ggcgtattct gcgcaatagc
     5461 agcttgttct acagtactag aagatgcaat agtactttct gttgtagtcg aagatgattg
     5521 ttcgttgcta cttgttgagc tagtagttgt tctagagtct ttagtttttt cctttttgta
     5581 gggttttaaa actaatttct ctttatcatc agatttatta gatttttctg gagtcaaaat
     5641 aatgtttttg tcttctttgc taacggtata cttggcatcg ttatccttgt ctttgtcgtc
     5701 tttccaagtc ataacattac cttcaagagt gtattcaaat ttataattca tttgatcaac
     5761 taggctttta gcaaattctt ctcccatggc gtcccactca tcgctagctg tagatttcat
     5821 tttgcttgtg tcaacactaa gagaaacgat atgatcagaa aatgatgcta tcatatttgg
     5881 ttcgtcatct ttggttgctt caatcaacca atcattagct tttaaatcat ctgtggttac
     5941 ttttttccca caagcagtga agagtagtag ggaagcaaga actaacagac ttcctatcat
     6001 ttttttcatt ttttattcct catttctatg atatgatttt tattggggaa tcttagaaat
     6061 aaggtttcga gtccgtgttg ctgcacgggc tttttttatt taaataaatc ccaaaaactg
     6121 aatgtagttt tcttgtatac tttattgtaa gcagcctttt ttggatcttt gatccaacca
     6181 gatccctttt taccgtaacc aggaatcact gcttttttca cagctctttt tgcctttcca
     6241 gtagttctag cgctaatgga tttttttata ctcggttttc tcattcctat tttcataaga
     6301 agcctccatt taaattacat taaggtacat agaagaagtg acatagttca caagtaattt
     6361 ttttatatat tcttcacaat cataaccaat tccataataa tccatgaatt tcatatagtt
     6421 gatttgatct acagataaag caaataaatt catataatct tgcaacagta cttcaatcat
     6481 gaatctattt gcttcatctt ccatttttga gtggaataca gttttgctgt aaagtgatat
     6541 caattcattg tgacttagtg catgacgtga ttcatgtagc aatacttttt tttgctctaa
     6601 ttcatctaaa ttttgattaa tgaaaattgt ccttaattta ggaagataaa atccttctga
     6661 ttcaatattg gtaatttcta catcaatccc attttccttc aacaaatctt caatgttatc
     6721 catacactac aacctcactc actttttcta ccttgtaaaa atatccttac cgcttcttta
     6781 tcttgatcgg taagtggttt tccgttccaa gtcattgctc catctaaagc atcatccaaa
     6841 tcggttggtg gtaattgaga atcactatcc tccgagttat cggttctacc tagtagataa
     6901 tcaacagata cattgaaata attagcaatt tcagtaagct tttcagcgga tggttgtttt
     6961 ccacttttta aactatagaa atagttttca ctgtatccta aatcaattgt tacttgtttc
     7021 attgtttttg aatgtttttt tgcaagaaat tttatccgct caaatactgt cataccagca
     7081 ttctcctttt ttctttacaa aaaaccaata aaaaagtgta gttttgtgtt gacctaaaca
     7141 acactatagt gtatattggt tttgtaagtt aattggatag aaaaaaagca aagtaaaaac
     7201 acaccttata gcattaagtt tggcgaccga gtgcgataaa aaaaggtttg ttataggctt
     7261 atttaactat ggctatatac tacactatag tatagttcgt agtcaactaa aaatatactt
     7321 ttctatccaa ttttctttct aaataaaaag aaaggaagtg tgtgaagtga gtaatatcga
     7381 taatgggcgg gaagccatca aaggatttat gaaagcaaat aatatttcag aatacgattt
     7441 ggccactgca tatggtagat cgagaacttg gattcagcgt gttttaagtg gaaaagataa
     7501 aggtccagct gttaacgcct ttattctgga agttattcgc gatcataaaa ttcgatagga
     7561 ggcaagtcat gaatattcta agcgaagagt ttctgactcg attgagaatc gcaattgttg
     7621 aagttgtaaa ggacgcactt agtcaacttt caaaaaagaa tttgtcagaa acacgatatt
     7681 taaaaaagat cgaagctaga aaatatgtgg gaggtgtaaa cgatcaaggc tttgagaagt
     7741 taatagctca cggtttaaaa gaaattcgta tagatggctt tttgagatat gacaaaaagg
     7801 acatcgatga actgatggct aaatacaaaa tttaaaggag gaaaaacatg ggaaagttta
     7861 atcgaacatt agtattcagc gcaccgctaa tcatctacgc tttaggactt tggggaagca
     7921 ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg cttgatgaag
     7981 ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa
     8041 agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa tttctctatt
     8101 gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt tagagtaaat
     8161 atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac ttcaaatgca
     8221 aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc agagtatatt
     8281 ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt agctgaccat
     8341 ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg ttctgttgaa
     8401 tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga agcattaagt
     8461 tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa cgtactcaat
     8521 ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt caaaataaac
     8581 accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc aagaattagt
     8641 aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa
     8701 aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt ccaattttgg
     8761 acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac gagttttctt
     8821 cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga aagctatcta
     8881 cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttat
//
LOCUS       DAFBYI010000099         8750 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFBYI010000099 DAFBYI010000000
VERSION     DAFBYI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.099"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..1305)
                     /locus_tag="LLD04_002203"
     CDS             complement(<1..1305)
                     /locus_tag="LLD04_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBK5649111.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASL"
     gene            1460..3019
                     /locus_tag="LLD04_002204"
     CDS             1460..3019
                     /locus_tag="LLD04_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK5649112.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(3130..5061)
                     /locus_tag="LLD04_002205"
     CDS             complement(3130..5061)
                     /locus_tag="LLD04_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5649113.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(5079..5993)
                     /gene="pfkB"
                     /locus_tag="LLD04_002206"
     CDS             complement(5079..5993)
                     /gene="pfkB"
                     /locus_tag="LLD04_002206"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK5649114.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(5990..6742)
                     /locus_tag="LLD04_002207"
     CDS             complement(5990..6742)
                     /locus_tag="LLD04_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5649115.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(6902..7111)
                     /locus_tag="LLD04_002208"
     CDS             complement(6902..7111)
                     /locus_tag="LLD04_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649116.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(7122..8450)
                     /locus_tag="LLD04_002209"
     CDS             complement(7122..8450)
                     /locus_tag="LLD04_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649117.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            8586..8741
                     /locus_tag="LLD04_002210"
     CDS             8586..8741
                     /locus_tag="LLD04_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649118.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         2447 a         1801 c         1602 g         2900 t
ORIGIN      
        1 aagagaagca aaactgcgta gtttttcgat agatcctggt ccgatgcctc gtttgggcga
       61 attaacgata cgctcaaaac tgattgaatc ttgaggattt accagcacat tcaaatacgc
      121 taaaatatct ttgatttctt tacgatcgta gaacttgtgc cctccaacca ttttataagg
      181 aatatttgct ttcaacagag tttcttccat tacacgagat tgtgcgttcg tccggtacaa
      241 gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca tccggtctac
      301 gataaaacgg gtttcgtctc gttcattatc tgcacggtag taagtgatct tgtttccttc
      361 tttgttttct gtccacaagt tttttggttt tcgattgcta ttgttttcaa tgacttggtt
      421 tgctgcgcta aggatgtttt tcgtggagcg atagttttgt tctagcaaaa ttactgcagc
      481 atctggataa tctttttcaa aatccaagat gttctgcata tctgcaccgc gccagccgta
      541 aatactttga tctgcatctc ccacgacgca caggttccta aatctacctg ccaataaatt
      601 gactagcgta tactgtgcgt gatttgtatc ttggtactca tctacatgaa tataatggaa
      661 tttgttttga taatacgtga gtgaatcagg atgttcttca aataatcgaa tcgtattcat
      721 gatcaggtcg tcaaagtcca tacattgatt atattgaagc tctttttggt aagcagcata
      781 acattttgcc gcaatctctt cgtaataact gccttgcatt tttgcaaaat cctgaggggt
      841 ctgcaggctg ttttttgctt gagagattgt tcctaatata cttcgaggat catatttttt
      901 cggatcaatg ttcagttctt ttagtatccg tttcatcaag gtcagctgtt cagaagaatc
      961 gatgattgta aagttccggt tatacccgat aaaatcaaca tctcttctta aaatacgcac
     1021 acacatcgag tgaaaagtcg atacccagac atcttcgcct ccagatgcga gaatcgcatt
     1081 gacacgttct ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca
     1141 aggattgact tctttttcct caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt
     1201 tccacttcct gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt
     1261 ctgacggggg ttcatcccgt ttaataattc agctttggat gacattaaca acatcctttc
     1321 ttttatcaat cttgttaatt atagcatttt cttggctttc attaaactag ctagttcaaa
     1381 atcatttgtt tctttttatg gatttttcct tgtcaattcg ttgatttcgc gctaaattaa
     1441 aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag ataagactca
     1501 gatatatttt attctaaaag gaatatttgt tggactgctg acaggaatcg tcgttagcct
     1561 gtttcgatta tctatcgaaa agctaagcga tttagttcga agtatctatg aaatgagtcg
     1621 ggaacagccg atttacttaa ttggcctagc tatttgctgc atccttgcag catttttcgt
     1681 gggttatctg gtcaaaaatg aaccggatat caagggaagc gggattccac aagtagaggg
     1741 acagcttcgc ggggaattat caatgaactg gttctctgtt ttatggaaaa agttcattgg
     1801 cggtgtactt tcagttggtg cgggcctttt tctaggtaga gaaggacctt ctatacagct
     1861 tggcgcatcc attggacaag gagctggaca attgtttcgg agtccatctt cagaagaaaa
     1921 gattttaatt tctagcggtg caagtagcgg acttgcagca gcattcaatg ctcctatcgc
     1981 aggattactc tttgttttag aagaaatcca tcactccttt tctccacttg tttggctgac
     2041 atcatttgct tcagcaatca ctgctaactt cgtctctctt tactttttcg gcttgagacc
     2101 tgttttatat ctaggtgacc ttccttcttt gccgttgaaa tattatggtt ccttagtgat
     2161 cttaggagta cttctaggca tcttaggttt tatttatcag aatgtcttgc tggctttgcc
     2221 aaaattttac aaccgattgc ctttaccagc ttattttcac gggttcgttc ctttcttgct
     2281 gattttgcca attggttatt tatggcctag tactctaggc ggaggcaatc aaatcgtttt
     2341 agcttttgga catgcttcct tgccaatcat tatgattatc ggtttgtttg ttcttcgatt
     2401 cgttttttcc atgatttctt acggtggggg tcttccaggg gggattttcc ttccgatatt
     2461 gacattgggg gctttaatcg gtagctttta tggaaacatt atggttgatt tcgggatgga
     2521 tccaatctac atcaaggatt ttgtcgttat tgccatggct ggttatttca cagcgattgg
     2581 aaaagcaccg ctgacagcaa ttattttagt gacagaaatg gttggctctc ttaatcatct
     2641 gatgccttta ggattggctg ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa
     2701 tccaatttac gaatcattat tggaacgtct acttggaaat actcagaccg cctcactaaa
     2761 aggtgcaagg acaacttttg attttccagt aacggcagag agttctttag acggattaat
     2821 ggtacgagac ttcaattggc ctaaaaatat gctgctaatc tctatacgca gaggaaatca
     2881 ggaaatcttg actcacggtg atacgatcat gaatgtagga gatattttgg ttatcttgac
     2941 tgatgaagca aacttgccaa gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa
     3001 tttgaagcaa tcattttaac gaacatggaa aaactatttt acaacaacta ttcatctaca
     3061 ctacttgatt ctatatgatt ctataaaaaa agaagaggct gggaaaaccc agcctcttct
     3121 tttttatttc tatgcttctg ctactgcagg agcttttctc aataaaccat aaatcacacc
     3181 agatacgact gctccgatag caataaacac caaatacatg accgggtgac tcagtaagaa
     3241 tacaacgaaa atcccgccgt gaggagccag taatttgatt ccgaaagctc cgactaatcc
     3301 gccagttaaa gcagaaccaa taataaaact tggaatggca cgaatcgggt cagctgctgc
     3361 aaatggaatc gatccttccg tgacaaacga tagtcccatc acgatgttcg tcaaaccagc
     3421 atcttgatct gttttgctga atttattctt aaacaaacgt gtagcaacga aaattgccaa
     3481 tggaggtacc attcctccag ccataacaga agccatcacg acacttccgc cagttgcaac
     3541 agtcgtagct aaagttgctg taccaaacac ataagcggct ttgttgattg gaccgcctaa
     3601 atctgccgcc atcattccag ctaataatgc accaagtaaa gcagcattcg aaccactcat
     3661 acttgtcaag aaaccgttca atgcatcatt gattgctttc atagggacat tgaccagcaa
     3721 catcaagaaa ccagtaatca gtaagccaaa cactggataa aagaggatcg ttttaattcc
     3781 atccaatgat tttggtaagt tcttgaataa tttacgtaaa aatacgatga cataaccagc
     3841 taagaatcca ccaatcagtg cacctaagaa tcctgcaccg cctgtattag ctaatgcacc
     3901 agcagcaaat ccaacgatca aaccagggcg gtcaccgata cttgaagcaa taaatcctgc
     3961 taataccggc agcatgaaac caaatgcagc ttgtccgatt tggttgaacc agctagcagc
     4021 ttgattgtaa ttccctagat ttgataattg atcatgtggc acgccaataa attggtcgat
     4081 catgaaagaa agcgcaatag cgattcctcc accaataacg aaaggtaaca tgtgtgatac
     4141 gccgttcatc aaatctttgt aaattctttg tccgatagta ccatccgcat ttccttcttc
     4201 tgaactgctt tcttttccat caccatggaa tactggtgca gtaccactaa gggctagatt
     4261 gatcaactct tctggtttac ggataccatc actgacagga cgattgatca gttcttttcc
     4321 gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg attacacctt ctgcacgggc
     4381 gatgtcttca gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt cgactttgat
     4441 ctccacaccc atttctttcg cttttttctt caaagcatct tctgccatgt aagtgtgagc
     4501 gatccctgtt ggacaagctg taacggcaac aacgaatttt ctttctggat gagcagaagc
     4561 ttctgcagct tgtgtttctt gttctgcttt ttcttctgct tctttttgct cttcagctgt
     4621 ttggaacaag tcttggactt cttctggtgt atttgcttct tttaattttc ctacgaaatc
     4681 tgcatcaatc agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc
     4741 ttctggagct gcgatcatga aaaacagata agttggctgt ccgtctaagg cttcataatc
     4801 tactcctttt tggctttttg cgaataaaac tgttgcttct ttaactgcac tgtttttcgc
     4861 gtgaggcatg gcgattccat caccaagacc tgtagaagtt tgtgcttcgc gggctagaat
     4921 tccttcttta tatgtttcga tatcggaaat ccggccggca tcaaacattt tttggaccat
     4981 ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac
     5041 cattaaatcc ttgatattca tgattatcct ccttgaaact aatcaatttt tgtgatttca
     5101 acttctggca gcaactcatc aatcaattcg cgagctgcca agtcatcaga aaaagctgtt
     5161 gcgcttccgc aagcaactcc ccatttaaag gcttcgattg gatctttcgt acggctgtaa
     5221 gccccaataa agccagcaat cattgaatcc cctgcaccta ctgaattttt caatgggcga
     5281 actaatacat tcgttttata agttccttgt tccgtgaaga gcaaagcacc gtctccagcc
     5341 attgagatca gcacattctg tgccccttca gctagtaatt ttttcccata cggaatgatt
     5401 tcttctacag attggaaggt tgtgttgtaa agctcagcta attcgtgatt gtttggtttg
     5461 atcaacaacg gtttcttaac aagtgcattt tttaggtcat cacctgttgt atcaatcacg
     5521 aaatctgcac ctttttctcg aatgatatca atcagttctt gataaaatcc ttttcgcagg
     5581 ctagctggcg cgcttcctga caatacgacg atatctcctt gtcctactgt tcctaatgct
     5641 tgcttcaact catcgatttc ttcatctgaa atagatgggc ctaagccatt gatctcagtt
     5701 tcagtgtctg attttagttt gatattgatt cttgtatctt cttttacggt tgtgaacaaa
     5761 gactggattt gttctttttc aagccacttc gaaataaatt cgccagtgaa ccctcctaaa
     5821 aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac attgatccct
     5881 tttcctccag gtaacttgaa gtcttccttc attcggttca aatcgcctag ttttaaccca
     5941 tctacgcgta cgatataatc aattgatgga ttaagtgtta ctgtgtaaat cattgtattg
     6001 cctccttaat tgttgtcttt tcacggaatt cattaacgaa ttctaatgga caacgctcgg
     6061 ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg
     6121 tatgatcaat caatacaaat gtctcttcgg aatttttcat ggctaaacgt ttaacagctg
     6181 cctcttctgg atccggagtc gtgaatcctt gttcaagatg ggcaccgttc attcccataa
     6241 atgctttatt aaaatgaaaa ttttttagtt gttctatcgc attagatcct aaaacagcat
     6301 tagttgccag ctttatcgtt ccacctaaaa taatggtagc aagctccaaa tcaactaaat
     6361 ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa
     6421 tcatttctaa cgtagtagag ccagcgtcta aataaatgac atcttcgtca ttaatggttt
     6481 cagctgccga tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat ttttcactca
     6541 tattctgctc aaaattcaat aattgctctt tcttagctcc gccatgaact ctttctagaa
     6601 acccactatt ctccagctct tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa
     6661 ggtttacaag atcttgtgat ttcacgattt tctgctgttc aagcaagtct aggatttttt
     6721 gtctgcgttc ctctgtaagc attttcacga tcacctcaat acaaataata gcatattttt
     6781 tttctactag caatataaat cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa
     6841 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     6901 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     6961 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     7021 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     7081 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     7141 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     7201 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     7261 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     7321 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     7381 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     7441 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     7501 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     7561 acaaaaaatg aagcattctt tttaggaacg ggttgcattt gtgcattctg atactggaca
     7621 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     7681 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     7741 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     7801 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     7861 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     7921 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     7981 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     8041 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     8101 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     8161 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     8221 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     8281 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     8341 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     8401 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     8461 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     8521 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     8581 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     8641 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     8701 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc
//
LOCUS       DAFBYI010000100         8749 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFBYI010000100 DAFBYI010000000
VERSION     DAFBYI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.100"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            6..305
                     /locus_tag="LLD04_002211"
     CDS             6..305
                     /locus_tag="LLD04_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649119.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LLD04_002212"
     CDS             378..1739
                     /locus_tag="LLD04_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5649120.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LLD04_002213"
     CDS             1761..2021
                     /locus_tag="LLD04_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649121.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LLD04_002214"
     CDS             2035..2787
                     /locus_tag="LLD04_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK5649122.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LLD04_002215"
     CDS             complement(2881..3486)
                     /locus_tag="LLD04_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5649123.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LLD04_002216"
     CDS             complement(3539..4513)
                     /locus_tag="LLD04_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK5649124.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LLD04_002217"
     CDS             complement(4891..5610)
                     /locus_tag="LLD04_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5649125.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LLD04_002218"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LLD04_002218"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5649126.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            complement(7271..8458)
                     /locus_tag="LLD04_002219"
     CDS             complement(7271..8458)
                     /locus_tag="LLD04_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5649127.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         2707 a         1612 c         1767 g         2663 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagaga ctaactatat agagtcaagt aaaaattagc caaatcaaaa aatattttat
     7201 aacagataat tccgaaaaaa gggtaaaaca aataagcttc ttaccattag tttttttgaa
     7261 tgttcaatag ctaatcagaa acaattatat aaggctcgtt tttagtcaaa atagcatgaa
     7321 tcacgtaaca aagcttacga gaaaccgcac ctaaggcagt tagatgatgt tttccttcgg
     7381 aaactttttt ctggtaaaac gcactaaaaa caggatcaca ttgagaagct actaaagccg
     7441 ccgaaaacaa ggcttttcgc aaatatggag accctcgttt actcataaca ttatgcgttg
     7501 cctcatattg ccctgattgt gtcacggaag cgtctatccc agcgtaagca acgagttttt
     7561 tcgggttaga aaatttttga atatctccga tctcaccaag aatagtcgca ccgtttatag
     7621 gaccaattcc aggaattgtg agaataacag aatttaaagt gtccattatg tgcttgattt
     7681 ctctttccgt ttcctttatt tggctttcaa tgaattttaa ttgttcaatc attgaacgta
     7741 gttggaaggt gaacgcttct tgagcaaatt ttactccaaa ggagttagaa gctgcttgct
     7801 tcaattggtc cgctttttct gccttgattc ggttcctgct taactcagct aagtattggg
     7861 ccaaagtttc tgaggaaatg ttttctaagt cgatcggggc actaaacgcc agcaataact
     7921 ctttagaagc ttttccgaaa acgccctgct tggtaaaaat cgtcgcgtat tctggaaaga
     7981 cttgatccaa tacggctata atttttcttt tgaagtcgct cgctgtacct actaaataag
     8041 ttcggtaacg agaaagttgt ttcaaagaaa aaacattttc atcggataag accgtttcga
     8101 caaatgagcc atagcgcatt aagtcagcaa tcaaaacaga atcaataata tcgttttttc
     8161 gtttccgtat ttctgtacct ttacgccatc cgtctgtttg aaggggattc acaacgtgaa
     8221 tcaaataatc tgattcgtga aggtatgaaa agatggacag ccaatagtgc cccgtggctt
     8281 ccatccctat aacaaagtca ctgggtgctc cggaatagtc gaccattcgc tcaattaatg
     8341 actctgctcc ttcagtggtg ttaggaaatg aaaaaccttt tagtaatacc tttccttcct
     8401 cgttcatgat agaagcaacg tgagtgcgtt taccgatatc aatgcctaaa aagtacatgt
     8461 atatctgacc tttcaaaaaa agaattttgg attgaaatcc actcaactga aaagcgtcac
     8521 taccttgtac gagatacgga gtaaagtaag gactttcaac atctaactca tacgcaatcc
     8581 acttatcaga gagaggagcc agtctctcga gtgcgggttc aatggcccaa ggggggaacg
     8641 gcttagcctc tcaatccaat aaatatatta tctcaaaaga aatgagttaa taccaaatta
     8701 aaataacgat ggttcatagg gttatccaat caaaaccgga aggttaaaa
//
LOCUS       DAFBYI010000101         8688 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFBYI010000101 DAFBYI010000000
VERSION     DAFBYI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.101"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            35..107
                     /locus_tag="LLD04_002220"
     tRNA            35..107
                     /locus_tag="LLD04_002220"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Phe,seq:gaa)
     gene            112..182
                     /locus_tag="LLD04_002221"
     tRNA            112..182
                     /locus_tag="LLD04_002221"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Gly,seq:tcc)
     gene            195..268
                     /locus_tag="LLD04_002222"
     tRNA            195..268
                     /locus_tag="LLD04_002222"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:229..231,aa:Ile,seq:gat)
     gene            280..351
                     /locus_tag="LLD04_002223"
     tRNA            280..351
                     /locus_tag="LLD04_002223"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:Glu,seq:ttc)
     regulatory      540..619
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            617..1810
                     /locus_tag="LLD04_002224"
     CDS             617..1810
                     /locus_tag="LLD04_002224"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK5649128.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1940..3412
                     /locus_tag="LLD04_002225"
     CDS             1940..3412
                     /locus_tag="LLD04_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5649129.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3495..4283
                     /locus_tag="LLD04_002226"
     CDS             3495..4283
                     /locus_tag="LLD04_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK5649130.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4350..5018)
                     /locus_tag="LLD04_002227"
     CDS             complement(4350..5018)
                     /locus_tag="LLD04_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK5649131.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5133..6845
                     /locus_tag="LLD04_002228"
     CDS             5133..6845
                     /locus_tag="LLD04_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649132.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6848..8620
                     /locus_tag="LLD04_002229"
     CDS             6848..8620
                     /locus_tag="LLD04_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649133.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2616 a         1477 c         1929 g         2666 t
ORIGIN      
        1 gagatcgcgg gttcgattcc cgtcggaacc gtttggctcg gtagctcagt tggtagagca
       61 atggattgaa gctccatgtg tcggcagttc gattctgtcc cgcgccatta tgcgggtgta
      121 gtttagtggt aaaaccacag ccttccaagc tgttgtcgcg agttcgattc tcgtcacccg
      181 cttttagttt ttatgggcct atagctcagc tggttagagc gcacgcctga taagcgtgag
      241 gtcgatggtt cgagtccatt taggcccatt tattttattg gcccgttggt caagcggtta
      301 agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc atggaagata
      361 ctttttgagt atctttttct ttttatctga aagaatgatt ttaaaaagct tagcagagtt
      421 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
      481 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      541 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      601 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      661 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      721 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      781 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      841 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      901 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      961 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
     1021 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
     1081 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
     1141 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
     1201 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
     1261 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
     1321 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
     1381 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1441 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1501 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1561 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1621 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1681 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1741 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1801 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1861 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1921 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1981 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     2041 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     2101 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     2161 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     2221 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     2281 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     2341 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     2401 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2461 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2521 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2581 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2641 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2701 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2761 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2821 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2881 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2941 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     3001 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     3061 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     3121 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     3181 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     3241 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     3301 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     3361 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3421 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3481 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3541 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3601 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3661 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3721 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3781 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3841 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3901 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3961 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     4021 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     4081 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     4141 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     4201 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     4261 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     4321 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     4381 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4441 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4501 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4561 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4621 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4681 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4741 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4801 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag
     4861 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4921 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4981 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     5041 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     5101 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     5161 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     5221 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     5281 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     5341 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     5401 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5461 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5521 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5581 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5641 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5701 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5761 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5821 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5881 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5941 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     6001 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     6061 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     6121 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     6181 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     6241 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     6301 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     6361 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6421 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6481 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6541 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6601 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6661 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6721 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6781 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6841 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6901 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6961 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     7021 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     7081 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     7141 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     7201 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     7261 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     7321 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     7381 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7441 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7501 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7561 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7621 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7681 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7741 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7801 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7861 aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7921 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7981 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     8041 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     8101 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     8161 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     8221 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     8281 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     8341 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     8401 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8461 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8521 tgatggggaa gtcattgaag aaggcagtca tcatgagtta ttagcagaaa atggatttta
     8581 tgcggaactt tatcataatc aatttgtatt tgaaagttaa attaaaagaa cctcgttata
     8641 acggggttct ttttatatat tttatgttgt ttgtttttat aaggggca
//
LOCUS       DAFBYI010000102         8621 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFBYI010000102 DAFBYI010000000
VERSION     DAFBYI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.102"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(142..417)
                     /locus_tag="LLD04_002230"
     CDS             complement(142..417)
                     /locus_tag="LLD04_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK5649134.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /gene="der"
                     /locus_tag="LLD04_002231"
     CDS             complement(660..1970)
                     /gene="der"
                     /locus_tag="LLD04_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK5649135.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="LLD04_002232"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="LLD04_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK5649136.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="LLD04_002233"
     CDS             complement(3516..4196)
                     /locus_tag="LLD04_002233"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK5649137.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="LLD04_002234"
     CDS             complement(4245..4880)
                     /locus_tag="LLD04_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5649138.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="LLD04_002235"
     CDS             complement(4946..6349)
                     /locus_tag="LLD04_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK5649139.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="LLD04_002236"
     CDS             complement(6336..7367)
                     /locus_tag="LLD04_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649140.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="LLD04_002237"
     CDS             7398..7619
                     /locus_tag="LLD04_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK5649141.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="LLD04_002238"
     CDS             complement(7784..8521)
                     /locus_tag="LLD04_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK5649142.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT         2389 a         1858 c         1461 g         2913 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa t
//
LOCUS       DAFBYI010000103         8576 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFBYI010000103 DAFBYI010000000
VERSION     DAFBYI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.103"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(312..842)
                     /locus_tag="LLD04_002239"
     CDS             complement(312..842)
                     /locus_tag="LLD04_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5649143.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="LLD04_002240"
     CDS             complement(1024..1680)
                     /locus_tag="LLD04_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBK5649144.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LLD04_002241"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LLD04_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBK5649145.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="LLD04_002242"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="LLD04_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="LLD04_002243"
     CDS             complement(2948..4162)
                     /locus_tag="LLD04_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK5649146.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="LLD04_002244"
     CDS             complement(4503..4745)
                     /locus_tag="LLD04_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5649147.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="LLD04_002245"
     CDS             complement(4777..5334)
                     /locus_tag="LLD04_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5649148.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="LLD04_002246"
     CDS             complement(5347..5535)
                     /locus_tag="LLD04_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK5649149.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LLD04_002247"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LLD04_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK5649150.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6531..6704
                     /locus_tag="LLD04_002248"
     CDS             6531..6704
                     /locus_tag="LLD04_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649151.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7344..7817
                     /locus_tag="LLD04_002249"
     CDS             7344..7817
                     /locus_tag="LLD04_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK5649152.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7826..8053
                     /locus_tag="LLD04_002250"
     CDS             7826..8053
                     /locus_tag="LLD04_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5649153.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1684 c         1274 g         3011 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     6421 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     6481 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     6541 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     6601 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     6661 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     6721 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     6781 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     6841 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     6901 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     6961 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7021 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7081 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7141 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     7201 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     7261 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     7321 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     7381 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     7441 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     7501 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     7561 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     7621 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     7681 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     7741 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     7801 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     7861 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     7921 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     7981 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8041 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8101 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     8161 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     8221 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     8281 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     8341 tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc
     8401 ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc
     8461 ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt
     8521 tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DAFBYI010000104         8560 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFBYI010000104 DAFBYI010000000
VERSION     DAFBYI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.104"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            363..731
                     /locus_tag="LLD04_002251"
     CDS             363..731
                     /locus_tag="LLD04_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK5649154.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="LLD04_002252"
     CDS             911..1852
                     /locus_tag="LLD04_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK5649155.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="LLD04_002253"
     CDS             1921..2652
                     /locus_tag="LLD04_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK5649156.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="LLD04_002254"
     CDS             2796..4976
                     /locus_tag="LLD04_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK5649157.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="LLD04_002255"
     CDS             4996..5478
                     /locus_tag="LLD04_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK5649158.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="LLD04_002256"
     CDS             5494..6255
                     /locus_tag="LLD04_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5649159.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="LLD04_002257"
     CDS             6386..7720
                     /locus_tag="LLD04_002257"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK5649160.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(7860..>8560)
                     /locus_tag="LLD04_002258"
     CDS             complement(7860..>8560)
                     /locus_tag="LLD04_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HBK5649161.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEP
                     LKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTI
                     SSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNF
                     RARIYLQQGLIFEN"
BASE COUNT         2826 a         1489 c         1885 g         2360 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcggctct tcgtcaaatc gtgttggtga
     7801 agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc gcatattttt
     7861 tagttttcaa agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg
     7921 cgataaccaa acgcaattcg tttaatcagc ttgatcttgt tattcagccc ctcagtgacg
     7981 ccgtttgagt gggacacctt aaaggcattt gaaatacccg attggtagcg atggaagatc
     8041 gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga
     8101 aagccttccg tacctcgttt aacgaaataa tagcgtaata attgacaaaa atgatacgca
     8161 gttcgcaatt cttcgttgta gcttaacagc tcatcaacga tttctgtttg actaattggt
     8221 cgtttaaaca gtcgatgata atgccgtttg agtggctcca atgtcgtgga atctttgagt
     8281 aagagtttcc aatatcgctt gagccgacgg tacttcttct gattttcaga gtgtgaggtt
     8341 cgaaaacgat tcatgatctg gacacgtaat tgattgaagc tgcgattgat gtgttggaca
     8401 atatgaaagc ggtcagttac gatctccgca tttggaaaga ctgttttaag caactgacaa
     8461 tagctggcat tcatgtccat cactaggtac ttgaccttta aacgggcctt tctaggaaag
     8521 cgcaggaagt attgagttag tttgtaaagt ctgcgatctt
//
LOCUS       DAFBYI010000105         8446 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFBYI010000105 DAFBYI010000000
VERSION     DAFBYI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.105"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            373..1812
                     /locus_tag="LLD04_002259"
     CDS             373..1812
                     /locus_tag="LLD04_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5649162.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2107..2325
                     /locus_tag="LLD04_002260"
                     /pseudo
     CDS             2107..2325
                     /locus_tag="LLD04_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2574..2789
                     /locus_tag="LLD04_002261"
     CDS             2574..2789
                     /locus_tag="LLD04_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649163.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3006..3262)
                     /locus_tag="LLD04_002262"
                     /pseudo
     CDS             complement(3006..3262)
                     /locus_tag="LLD04_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3449..4360)
                     /locus_tag="LLD04_002263"
     CDS             complement(3449..4360)
                     /locus_tag="LLD04_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5649164.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4379..5383)
                     /locus_tag="LLD04_002264"
     CDS             complement(4379..5383)
                     /locus_tag="LLD04_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5649165.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5380..7503)
                     /locus_tag="LLD04_002265"
     CDS             complement(5380..7503)
                     /locus_tag="LLD04_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5649166.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7508..8428)
                     /locus_tag="LLD04_002266"
     CDS             complement(7508..8428)
                     /locus_tag="LLD04_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5649167.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2426 a         1719 c         1560 g         2741 t
ORIGIN      
        1 ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc
       61 aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg
      121 agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag
      181 aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc atctccaact
      241 tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc
      301 gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa
      361 ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg
      421 attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat
      481 attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta
      541 gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat
      601 agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg
      661 ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt
      721 ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat
      781 gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga
      841 ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat
      901 gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc
      961 atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc
     1021 ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta
     1081 ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc
     1141 tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca
     1201 gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa
     1261 aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg
     1321 atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg
     1381 gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg
     1441 gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg
     1501 ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg
     1561 gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct
     1621 acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc
     1681 gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt
     1741 ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag
     1801 aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg
     1861 gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta
     1921 tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc
     1981 cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta
     2041 tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat
     2101 tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt
     2161 ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt
     2221 cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag
     2281 ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag
     2341 cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc
     2401 cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta
     2461 ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca
     2521 gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg
     2581 ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa
     2641 tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat
     2701 cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg
     2761 gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc
     2821 tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca
     2881 agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa
     2941 ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag
     3001 tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt
     3061 gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt
     3121 gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa
     3181 tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat
     3241 gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca
     3301 tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat
     3361 tcatcaccca cctatagagg atgggcgact tctgcctgaa ataagttaca ctacttataa
     3421 cttctaataa aattagattg agtcattatt atttaacatc aacgattttc cagtttccat
     3481 ttttttgtaa tactaatttg tactgaaagt agctagtggt ttttgataca ttatcaaggt
     3541 attttatctt tgtactcact agaaaatttt ttccgttttg tagataaact ggattctcta
     3601 actccgaaaa ctttaagtca atattgattg gtcgcatcgc accattttca acataatact
     3661 ccaattcttt ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa aaatcggtga
     3721 tttcttccgt tgttttggca tcaatttctg aatcgttttc tacttgtttt gcttcatatt
     3781 ttgccttgtc tggctttcca atcattgttg gcaaactagt tactaaatga ttcctgtttt
     3841 catcttcaaa gattgtcact cgatatgcag aagaaattga ttgcgtcttt tttccctctg
     3901 taatatgttg ctccactaaa aatgaaacga caaattcttc cgagtgtttt tcaacatcta
     3961 gaatttttac agattgaact tcggaactcg tgggaatatc tgctcgaacc atatcttgga
     4021 ttaaagcgag accgttttct gtaaggtact gttcgagatt actcattctc ttttcaattg
     4081 cttctttgtt atttttccat gaaaaatatt ctttcgcaaa atcctctgta aaactttcaa
     4141 cgccagaagt attcactacc ttctctttta taaccacttt ttcatgagtt gtatgttgat
     4201 caatggcggt gaaattttta taaacgccaa aagcaagact acaagacaat aaaagccaaa
     4261 gggcaagcac catttttcga tgcttaccca cggatacaac tcgttctttt ttcttttttg
     4321 ggctcttttc cttttgattc cgttcaattt ttatcttcat tctttttcct cttttctctt
     4381 atttgtatcg gccagctccg ataagatgct gccgccaata gttttccgtt agattcgtcc
     4441 accccactgg atctcctgca tgaaacatgc gattctcccc tgcgtaaatg cccacatggg
     4501 tcacataagt accggcatca taggttgaat gaaagaaaac taaatcacct ggtttggcat
     4561 cttttagatc aatatgagtc atggcatcat attgatcctg agccacccga ggaagtttca
     4621 ggtcaacttt ggcataacac cattgggtca agccactaca atcaaacccc gtttctggac
     4681 ttgagccacc aaacacatag gccgttcctt catacttaaa tgcctcatca aaaatttctt
     4741 ggactatttt gtcatcaaac tgagttgtag ttaggtattg cttcactaat aaacagtaaa
     4801 acatattgcc ataattatac cgccacccac cattttcctt aatcgcgatt gggttggggt
     4861 aatcgacttt ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat
     4921 gcttcttccc gtttttcccg acatgatcta gatacccgcc accatagtta taggcctgaa
     4981 taatactatc ttgatcacac cctagttctt tggaacgctt aaccaaagac gcaaaataga
     5041 gacagccttg tttgatggat gcctcggtac ttaatgaatt aggtggtaat ccggctgact
     5101 cagaactttg cataacgtct tccattgtgc caccagattc cacttgaata atcgccaaaa
     5161 gaattggaat atactctgtt atatccgatt ctttggcata cttttccacc attgcacggt
     5221 gtttcaatac ttcttctgag acactagttc cgccaggaga agtgccagaa ctaccatttc
     5281 cctcactgtc ttcaccaaac aacaaaccca cacacatcaa aatacccata aaagagatgg
     5341 tcagaaaagt taatgaaata acacccactt tcttcaagtt cattttttct cacctgacct
     5401 tgattttgga cgatggttca ttcttttttg tactttttga ttggtctgct ttggcagagt
     5461 ggacctctgt ttcacttgtt ggaaagaatt tttcttactt gctgattggc ttttcccttt
     5521 tgaaatgatt tcttgactag ttagttttcg tttgatttcg ggattcagat ttgctctcac
     5581 aatccgcttt ggtgcttctt ttttcagcat catatctttc tgaggttgag ctgctttatc
     5641 tattggaata ttcgcccttc tttttccctg acgatttgtt gcttcactca gtatcttgcg
     5701 tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat ccactcgatc
     5761 cgctttttct tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg acttttcgat
     5821 tcctttatgg agattgtatt tcaaattcgt tggcgtattc cttacctgat cctttacttg
     5881 tttagtctta tctttcacca aagcttgggt atccaaaact ttgccgattt ttctcccagc
     5941 caaattcatt cgggattgtt tcttattatt tgaaactgga ttctccttag ttaagtcaga
     6001 tagtatttcg tgatcgttcg gtaatcgtga attctttcgt agagaagaac cggatggctt
     6061 aaatttggat tttgattgtt cctttgccaa cttatttccg actgctgcac caccaccaaa
     6121 agccaaagct cgtcccacat ttcgattgag ttgccgcaat tttcgattca ttaacatttg
     6181 cggttttcgc atgacgcgtc ttcccattga ctggctatca ttactctgga ggttgaacat
     6241 actcataatg tcgcccaatt tgaaatagat accggcaaag gtcacaattt gcagaaaagc
     6301 aaccatgaaa aatggatagg ttgccgaaat attgaaaaac atcgtggaga tgctaaatgc
     6361 agtcgtaatc accaaagtaa cacccgctcg catcataatc gtattgaata aacgaataat
     6421 cgccttcttt cctaaacttt cataagtcgg caacatggac aataagaaac taattggcag
     6481 aaacatcgca aagataatga agagaatttg cgagaacaac ataatccctg ttagaagaaa
     6541 aacaaagaag gaaatgccaa tattgaacaa taccaaaaag accacggtcc ctaaacggtt
     6601 catggtcttg gtaatactga gattcgcatt gtcattgtct tcaatctctg ccttgaccgc
     6661 ttcttcccga tctttgcctt tgttttcatc tggactcaca gatagaatct tgttgacccg
     6721 gtcttcacca atttcttcta tgttggaatc atcaaattgc agtagtaacc aaggttgttg
     6781 gacttgaatc gaaaacaaac tatcccgaat caaatccaca ctatcttttc cttggctttc
     6841 tgaattgggt acaacgatct cggttccaag ggtaagggcc gcttcactaa catctgagct
     6901 aaagtcattg atttttgtga tataggtagg cgcataagca atgaacgaac ccgatagtag
     6961 aaaaatcatc acaaaattaa tcacggctcg aactgctttc gtggtttctc gttttaataa
     7021 tccggtatac gccacataca cccccagagc taaaatcatc aataatagaa acccaaaata
     7081 aaatccggag gtctggagtc cattttcagt aataccagcc aatgtttgga tattctttcc
     7141 aatactttca gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa ccacataacc
     7201 cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg cataaagacc
     7261 atacatcacg cttttcccta agccatcacc ccaattccaa ggcaaccaat cccaagagct
     7321 atccacaaag aaatctagtt gatagttgtt cagtgaatat ttggaatata gatttcctgc
     7381 ttcaactgta tcatcaacta gccctgtcgc ctcggctacc gtaccaacta aactcaatag
     7441 aactatcaga gtgagtatgc taataaaaac gattcccaca atccgaaaaa gtttcttttt
     7501 catcccctca ctccattctc tcttgaacag gtggacgggt atcaaaggca tggaagagtt
     7561 cttcaaagac tggatgtacc tgaaccacgc ctacccgacc atacaaatcc tgcatcaagc
     7621 attggccatt ttccaaatct cgtaggcgtt tttgattgct ttcatcttct cgatccaagc
     7681 caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaac gcaaacttca
     7741 acccgatatt attcttcaag cgttcatcca gtaaatcatc actattttgc gtgacaaagt
     7801 aaaccccggc attcatagcc cgcccagcac gaacgagctt attcgataat gttttccctt
     7861 gcgctacttg aagaaaactc cacgcctcat ctaagtctac aattttgaag atttctcgat
     7921 tactatggat aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa acactaagca
     7981 attccatggt ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc aaatccgcta
     8041 cttgaatgat atttaactgc ctatctaaac taatcgactg ggtaatcgaa ccatctgaga
     8101 aaagcaaatg ggcaaaatca taatcggtaa aggactcgat gtgatccgcg atattttccg
     8161 caactggtga gccttctttt cgaagctcat taattacttg taataatccc cgttgattgc
     8221 tttgggtaac cgtccgaatg gccttacgca acactggaaa cttctcccca tctcgactag
     8281 aaattcctgt taaaaaggtc agaatatcaa ttgctagact ttcagaatcc ttctttttct
     8341 ccataatcac ataaggatca agtaatcctt gattttttgg cttactagtt aagttgagaa
     8401 tattgatttc atccgcaatt tctggcaagg tttctttcca gccacc
//
LOCUS       DAFBYI010000106         8424 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFBYI010000106 DAFBYI010000000
VERSION     DAFBYI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.106"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            470..2107
                     /locus_tag="LLD04_002267"
     CDS             470..2107
                     /locus_tag="LLD04_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649168.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LLD04_002268"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LLD04_002268"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5649169.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="LLD04_002269"
     CDS             complement(3183..3818)
                     /locus_tag="LLD04_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5649170.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LLD04_002270"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LLD04_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK5649171.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="LLD04_002271"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="LLD04_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6416..>8424
                     /locus_tag="LLD04_002272"
     CDS             6416..>8424
                     /locus_tag="LLD04_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5649172.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2784 a         1547 c         1293 g         2800 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
      421 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
      481 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
      541 tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
      601 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
      661 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
      721 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
      781 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
      841 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
      901 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
      961 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
     1021 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
     1081 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
     1141 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
     1201 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
     1261 gacacacatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
     1321 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
     1381 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
     1441 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaat
//
LOCUS       DAFBYI010000107         8422 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFBYI010000107 DAFBYI010000000
VERSION     DAFBYI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.107"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            818..2428
                     /locus_tag="LLD04_002273"
     CDS             818..2428
                     /locus_tag="LLD04_002273"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK5649173.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2720..3589
                     /locus_tag="LLD04_002274"
     CDS             2720..3589
                     /locus_tag="LLD04_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK5649174.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3754..5025
                     /locus_tag="LLD04_002275"
     CDS             3754..5025
                     /locus_tag="LLD04_002275"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5649175.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5022..6317
                     /gene="rho"
                     /locus_tag="LLD04_002276"
     CDS             5022..6317
                     /gene="rho"
                     /locus_tag="LLD04_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK5649176.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6416..6679
                     /locus_tag="LLD04_002277"
     CDS             6416..6679
                     /locus_tag="LLD04_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK5649177.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7124..7573
                     /locus_tag="LLD04_002278"
     CDS             7124..7573
                     /locus_tag="LLD04_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5649178.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7630..>8422
                     /locus_tag="LLD04_002279"
     CDS             7630..>8422
                     /locus_tag="LLD04_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBK5649179.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFV"
BASE COUNT         2810 a         1442 c         1817 g         2353 t
ORIGIN      
        1 accgtttatc cagaggttgt gagcctacca tttaactccg aatcacaaga aataatttca
       61 aaaacagctt ctcatgtttg atggatattg tgacttgtga ccgaggagtt ggcaggagaa
      121 caccgtttat ttagaaataa ggaggcacga cttttgtcgc atctccttat tttttactta
      181 tgtgattcgt taccttcaat gaatccctac aaggcacttt tgttgaaaaa aaggtcatct
      241 ttgaaaaaat tagtggaatt gttttgtttt tgatattatt taatagaaaa gatatgccaa
      301 aaatccgaga actgtggact acgtttttta cgtaaaggag gtaaataagg gtgaaagaaa
      361 aagattgttt ctacgataag ctatataaac tgttgctgca agtaggaaaa agtagttctt
      421 tgttaggtgg gatattatct atcataatgt ttgttttatt tttttatcta ttatttcact
      481 taaaattttt cagtaacgat tttctgattt gcttaggttt aacgttagct atgtttatat
      541 caagcttatt atgtgggtta aatttttttt gcactcataa aggacttaag aaataatttc
      601 tcaaaggata tcgaaaaaga aaaaattgta agcggatagt attcatttga gtacttgact
      661 ttcccttagt cagcttgtat tatacacctt gggcaccctt ttagagggtc agaagtttac
      721 agaaatttcg gctccctatt cttattgaag aatagggagc tttgtttatt gtctgagact
      781 tttcctgcac ataaattaaa aaggagtttt tgaacttatg acaaaatata tttttgtgac
      841 cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca gcttctttag gacgcttatt
      901 gaaaaaccgc ggcttaaaag taactatcca aaaatttgat ccatacatca acgtggatcc
      961 ggggacgatg agcccatatc agcacggaga agtattcgta acagatgatg gtgctgaaac
     1021 agatttggat ctgggccact acgaacgttt tattgacatc aatttgaaca aatactccaa
     1081 cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa gaacgtaagg gcgaatactt
     1141 gggagcaact gttcaagtta ttcctcatat tacaaatgag atcaaagaaa aaatcatgcg
     1201 agcagctaaa atgactgatt cagatgtgat cattacagaa gtagggggaa cagtagggga
     1261 tatagaatct ttgccattcc ttgaagcact tcgtcaaatg aaagcagatg tcggtacaga
     1321 taatgttatg tatatccata caacattgat tccttacttg aaagctgctg gagaaatgaa
     1381 aactaaacca acgcagcaca gtgtaaaaga gctacgtggt ttaggtatcc aaccgaatat
     1441 tttggtggta cgtacagagc aacctgtttc tcaaagcgtg aaaaacaaat tggcacaatt
     1501 ttgtgatgtc gaaccagaag cagttatcga gtcgccagat gtagatacac tttactccat
     1561 cccattagtt ttacaagcac aaggaatgga tcaaattgtt tgcgaccatt taaaactaga
     1621 tacaccagaa gcagacatga cagaatggaa agaattagaa gaacgtgtct tgaacttgaa
     1681 gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag ttaccagatg catatatctc
     1741 agtagttgaa gcattgaaac attctggttt cgcctataat tcagacatcg agatcgattg
     1801 gatcaaagca caagaattaa cgagagaaaa tgtagaagaa cgtttgaaag acgcagatgg
     1861 aatcttggtt cctggtggat ttggtgatcg cggagtagaa gggaaaatcg aagcgattcg
     1921 ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt cttggtatgc agatggcttg
     1981 tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg tcatcagctg aaacgaatcc
     2041 agatacagca aacaatatta ttgatttaat ggctgatcag gaaaacatcg agaatttggg
     2101 tggaacttta cgccttggat tatacccatg taaaatcaaa aaaggaacga agacggctga
     2161 agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt caccgctatg agttcaataa
     2221 caaatatcgt caattgtttg aagaacaagg gctagtcttt tcaggtgtct caccagataa
     2281 ccgtttagta gaaatcgtag agctgccaga gaaaaaattc tttgtgggat gccaattcca
     2341 cccagaattg atttctcgtc caaatcgccc gcaaaaatta atcaaagcat ttgtcggagc
     2401 atcattagca gatcacgaaa caaaataatt tttgaacttg atagaaagag aaaagtagca
     2461 agagaagggg ataagcaatt ttcttgtcac ttttctcttt ttctgatttg ttattatttg
     2521 taggaaaaaa gatagaaagc aaaagacttt tttcgattaa agcgctattg gatattttat
     2581 tttttgcaaa aaacttcttg aaaattttca ttttcattga ttttagtaga aaactaccga
     2641 aatgtttggt aaaatagggt cgttggttaa gaattgtctt aacaaattta gttttacaaa
     2701 acttaggagg aattttatta tgccagtagt atcaggagct gaatttttaa aagctgctcg
     2761 taaaggcggt tacgctgtcg gcggattcaa cacaaacaac ttggaatgga ctcaagcaat
     2821 ccttgaagcg gctgaagcaa aacaagcacc agtacttatc caaacatcaa tgggtgcagc
     2881 taaatacatg ggtggttaca aaatctgtaa agatttaatc actgatttag ttgaagcaat
     2941 gaacatcact gttccagttg ctatccactt agaccatggt gattacgaag ctgctttgga
     3001 atgtatcgaa ttaggctata cttcagtaat gttcgatggt tcacatctac catttgaaga
     3061 aaacttgaaa ttagctaaag acgttgttga aaaagcacat gcaaaaggta tctcagttga
     3121 atgtgaagtt ggttcaatcg gcggagaaga agacggaatc atcggtaacg gcgaattagc
     3181 tgatattgaa gaatgcaaac aaatggttgc tacaggtatc gactacttag cttgcggtat
     3241 cggtaacatc catggtcaat atccagaaaa ctggaaagga ttagcttttg accacttaca
     3301 agcaattgct gatgctgtag gtccagacat gcctttagta ttacatggcg gatcaggtat
     3361 tcctcaagat caaatcgaaa aagctatctc aatgggtatc tcaaaagtaa atgttaacac
     3421 tgaattccaa ttatcatttg ctgcagctac tcgtaaatat atcgaagctg gtaaagataa
     3481 agaaggtaaa ggattcgacc ctcgtaaatt attagctcca ggtaaagcag caatcgttga
     3541 agatgctaaa tctcatatcg actggttcgg ttcagctaac aaagcgtaag catccacata
     3601 acagatacga tatataggaa tggagaacga cttttgaagt cgctctccat tcttttttac
     3661 ttaaaacggc agaccatgct cgtttctttt ttttatctat gataaaatac aagcatatcc
     3721 atgaaaaaag attaataaac aaggtggaaa cagatgaaaa aaatcgtaat caaaggaaac
     3781 cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa atagtgcagt cgcattgatt
     3841 ccagctgcaa tcttagctga ttccccagtg atcttggaag gtgtgccgga catacaagat
     3901 gttcattcat taattgaaat tttagagatc atgggcgcaa ctgtccattt ttccaacaac
     3961 atactagaaa tcgatcctcg agaagtcgta tcgattccga tgccgcacgg aaaaatcaat
     4021 agtttgcgtg cttcttatta ttttatggga acactgcttg gtaaatttgg cgaagcggta
     4081 gtaggcctgc caggtggctg ttatttaggc cctagaccga ttgacctaca cgtcaaagga
     4141 tttgaagctc taggtgctga agtgacaaat gaacatggtg ccatgtattt acggacagaa
     4201 aataaaggat tgcgtggcaa tcggattttc atggacgtgg tttcagtcgg agcaacgatc
     4261 aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag taattgaaaa cgctgcacga
     4321 gaaccggaaa tcatagatgt ggcaacttta ctgaataata tgggggcaaa agttcgagga
     4381 gcgggtacag atgtgatccg tatcgaagga gtcgaaacat tacatggctg tcggcatttc
     4441 atgattcctg accgcatcga agcaggaacc tatctagcat tagcagctgc agtaggaaat
     4501 ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa gctttatagc aaaattgcag
     4561 gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg aagtttaccc ctcgaaagaa
     4621 ttaaaacccg caaacatcat gacctatcct tatccaggat ttgccacaga tctgcagcag
     4681 ccacttactg cgttactatt gatgactaca ggcacttcag aaatcatcga tacgatctat
     4741 gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg gcgctgatat taccgtggaa
     4801 ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg gcacagaagt cgtggcttct
     4861 gatcttcggg caggtgcttg tttagtgatt gctggactat tggcagaagg gacaacgacg
     4921 atttacaatg tagattatat cttacgcggt tatgatcata ttatcgagaa actaacagcc
     4981 ttaggggcat caattgaaat gatagaggaa gtgggagctg aatgagcgat tatttgacaa
     5041 tggcagaact agagaacaaa acgttaaaag aagtttatag ttatgccaaa gaatttaaaa
     5101 ttccttatta cagtaagatg aataaaaagg aattatctct agctgtgatt cgtgcacaag
     5161 cagagaaaca aggattttat tatatggaag gaatattgga tatcgttggt caagatggtt
     5221 acggtttttt acggccaatc aattatggac caagtgcaga agatatctat atttctgcct
     5281 cacaaatcag acgattcagt ttgagaaatg gggacaaagt ggcaggaaaa gcccgtccac
     5341 ctaaagaatc ggaacgttat tatggtttga tgcacgtgga aagcgttaat ggaaaagatc
     5401 cggaagaagc aaaagaacgt ccgcattttc cagccttaac acctctttat ccgcaaagac
     5461 agcttcaact ggaaacaaca ccgcaaagac tatctacccg aatgatcgat atcttttctc
     5521 caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc aaaagcaggg aaaacttctg
     5581 tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc ggaagtagaa ttaatcgttc
     5641 tactgattga tgaacgaccg gaagaagtaa cggatctaga acgaagcgtg aaaggcgacg
     5701 tggtatcttc tacatttgat ctgcagccgc aaaaccatac tcgagtttct gaattagtat
     5761 tggaacgcgc aatgcgttta gtcgaagata aacgagatgt cgttatcttg atggatagta
     5821 ttactagact tgctcgggcc tataacttag tcgttccacc aagcggacgt acattgagtg
     5881 gcgggattga cccagccgca ttgtataaac ctaagaaatt tttcggggca gcaagaaaca
     5941 tcgaagaagg tggcagtttg acgattttag cgaccgcttt agtcgataca ggaagccgta
     6001 tggatgacgt gatctatgaa gaattcaaag gaactggaaa tctagaatta caactttcac
     6061 gtgaattatc tgaacgtcgg atcttcccag ccatcgatat caaaaaatca ggtacccgaa
     6121 aagaagaatt attgatgcca agtgaagaat tagaagaaat ttggaaactt cgaaaaaata
     6181 tgactggaga ttcattagaa tatacggaac aactgatccg ctttttgcgt aaaacaaaaa
     6241 acaatcagca atttttcaaa gaatttcaag atgtctcctt tggtaaaaaa aatccgacca
     6301 gaaatctgaa aagataattg caaccttgac tgaaacatgt taagatgtta atgttgtaaa
     6361 tcgacaaatc aactctggtt cagcaaatgg agcagggcaa aggagcgaaa aaatcatgaa
     6421 agaaaacatc catccagatt accacccagt agtattcatg gattcaacaa ctggtttcaa
     6481 attcttgtca ggttcaacaa aaacttcaca agaaacagtt gaatgggaag acggtaatac
     6541 atacccagtc atccgtgtcg aagtaacttc tgactcacat ccattctaca ctggacgtca
     6601 aaaattcaca caagcagacg gacgtgtgga ccgtttcaac aaaaaatacg gtctcaaaga
     6661 cgcaaacgct gcggaataat aaaaacgctg ataactcaac gtttagcaga ctatctgata
     6721 gggatagtct gtttttttat gttttttgcc aatttaaaac ttcgtttgat gctgaaaaag
     6781 tcgtaggatc ataatgcaca aaacaatgaa aaactagtat atagttttaa ttatccactt
     6841 gataaaaaca ccttatatta acgaaatgaa gaggggttat ttcgaagtaa aaaaccattt
     6901 acctaaaatt agaagctaag ctttcctttc aaggaaggct ttttgttttg ttatagactt
     6961 gcgattgttt gtgagctaga tcgttcattt taatgactta tacacgcgga tgatgggtct
     7021 ttttcctctg ttcattcata atattgaacg gtacagctag cgatgctata ctgacctcag
     7081 ttccaagaaa acgttaacat tttttgtggg ggagaggttt tttatgaggt ttgatcaatt
     7141 gatcgcagaa gaactgattg ttttcgatcc atcgatagat tcaaaaggat cattatttga
     7201 gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga aatgttcaaa aagtcgttaa
     7261 ggcactattc aaacgagagg aggagatctc cacaggaata gaagatggat ttgggattcc
     7321 ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt ttttttcata cagccaaatt
     7381 aacggactat agcgggatag atgaaatgcc aattgaatat atttttgcca tcgtagttcc
     7441 tgaaaaatca gcagatatgc atctagagat tctcagcaca ctctccagac gattgatgaa
     7501 tgcagatttt agaaagggaa taaaagaagc cactacgcca gcagatatca tcagacagat
     7561 tagtcaagaa tgaaaatagt agagtctttg tccgagtatc gatagtgaag aaaaaggagg
     7621 aaaataatca tgaaaatcgt tggtgtaact gcttgcccga ctggaattgc tcatacttat
     7681 atgtcggcag aaaaattaga agtaacagca agaaatctaa accatcaagc aaaattcgaa
     7741 acacaaggtg taaaaacaga aaatcagtta tctgaacaag aaatcaaaga ggcagatgca
     7801 atcattcttg cagtagataa agaaatcgaa ttggaccgat ttgccgggaa aaaagtaaag
     7861 cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag taatagacga agcattgaga
     7921 gatattggcg tattcgttgt gtctaatgaa aaagaaccag ctgcaaatga gaaaaagccg
     7981 acaatctata atcatttcat gaatggtgtg aattacatgc ttccatttgt gatcgcaggc
     8041 ggaatcatca tcgcactgag ttttgccttt gggattacag cggctgatcc caactcagca
     8101 gactataatg tgttagcagc tgcattttca aggatcggag gagatacagc gtttgccatg
     8161 atggttcctg cgttaggcgc ggggattgct acatcgattg ctggaaaagc aggttttgcc
     8221 ccaggaattg ttgccggatt actggcctct acaggtggtt caggttttct tggcggaatg
     8281 attggtggcg tgctagcagg gtatatctgt gattttctgg caaataaaat tagcgtaaaa
     8341 aaagaatttt ctgctattta tcagctgatt gtcgtcccct ttttatcgat cttaattatt
     8401 gggcttttga tggtatttgt ga
//
LOCUS       DAFBYI010000108         8296 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFBYI010000108 DAFBYI010000000
VERSION     DAFBYI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.108"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(51..1517)
                     /locus_tag="LLD04_002280"
     CDS             complement(51..1517)
                     /locus_tag="LLD04_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK5649180.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="LLD04_002281"
     CDS             1904..2500
                     /locus_tag="LLD04_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5649181.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="LLD04_002282"
     CDS             complement(2558..3040)
                     /locus_tag="LLD04_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649182.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="LLD04_002283"
     CDS             complement(3146..3343)
                     /locus_tag="LLD04_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649183.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="LLD04_002284"
     CDS             3638..4396
                     /locus_tag="LLD04_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK5649184.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="LLD04_002285"
     CDS             4456..4884
                     /locus_tag="LLD04_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649185.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="LLD04_002286"
     tRNA            4930..5002
                     /locus_tag="LLD04_002286"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="LLD04_002287"
     CDS             complement(5152..5523)
                     /locus_tag="LLD04_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5649186.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="LLD04_002288"
     CDS             5738..5923
                     /locus_tag="LLD04_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649187.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="LLD04_002289"
     CDS             complement(6006..6194)
                     /locus_tag="LLD04_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649188.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="LLD04_002290"
     CDS             complement(6218..6481)
                     /locus_tag="LLD04_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649189.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="LLD04_002291"
     CDS             6639..6896
                     /locus_tag="LLD04_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK5649190.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="LLD04_002292"
     CDS             complement(6967..7158)
                     /locus_tag="LLD04_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649191.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="LLD04_002293"
     CDS             complement(7299..7619)
                     /locus_tag="LLD04_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649192.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="LLD04_002294"
     CDS             complement(7642..8076)
                     /locus_tag="LLD04_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5649193.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DAFBYI010000109         8287 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFBYI010000109 DAFBYI010000000
VERSION     DAFBYI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.109"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="LLD04_002295"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="LLD04_002295"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK5649194.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="LLD04_002296"
     CDS             complement(1023..2126)
                     /locus_tag="LLD04_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK5649195.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3172)
                     /locus_tag="LLD04_002297"
     CDS             complement(2216..3172)
                     /locus_tag="LLD04_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK5649196.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="LLD04_002298"
     CDS             3453..5387
                     /locus_tag="LLD04_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5649197.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5844..6260
                     /locus_tag="LLD04_002299"
     CDS             5844..6260
                     /locus_tag="LLD04_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5649198.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6274..6840
                     /locus_tag="LLD04_002300"
     CDS             6274..6840
                     /locus_tag="LLD04_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK5649199.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6863..7849
                     /locus_tag="LLD04_002301"
     CDS             6863..7849
                     /locus_tag="LLD04_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5649200.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8004..>8287)
                     /locus_tag="LLD04_002302"
     CDS             complement(8004..>8287)
                     /locus_tag="LLD04_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5649201.1"
                     /translation="SDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTN
                     KTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2451 a         1601 c         1668 g         2567 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5581 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5641 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5701 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5761 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5821 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     5881 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     5941 tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg cgtgtggatg
     6001 gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca tatacttaca
     6061 aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta cggataaaag
     6121 aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa aatggcgaaa
     6181 ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat ggaggtgacg
     6241 aagacagtct gatttattag accttcacac accatgagta aattacaaaa aatgatttat
     6301 atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat gatcccttat
     6361 cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac gatcgttgcc
     6421 atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact attcctgact
     6481 actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc tttgcttagt
     6541 tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat catcggaatt
     6601 agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc tttcattgca
     6661 caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat tttatcaggg
     6721 attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact tcaacgattc
     6781 caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac caaaaaataa
     6841 tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact atcctgcttt
     6901 aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc tgaaaaacaa
     6961 acctgtcgaa caagcaatca aagaaacgat ccatgccggc ggaaagctgt tgcgtcctgc
     7021 ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag ccatcgcttt
     7081 ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata tcgttgatga
     7141 agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg tcgtttctgt
     7201 ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt actccacttc
     7261 cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg gcgaattagg
     7321 gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg aaaatatttc
     7381 aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg aaagtggaac
     7441 aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga tggcctttca
     7501 gattatcgat gatattttag attatacaaa agagagccag gaaatcggca aacctgtact
     7561 agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc aaaaaaacaa
     7621 accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag atgtcgacgc
     7681 cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt tagcggcctc
     7741 ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc ttcgaacaaa
     7801 agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa tagggaaagg
     7861 ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca aattgaattg
     7921 tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta gcgacgttgc
     7981 tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat aaaagtgatt
     8041 cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt cgattgactt
     8101 ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt tttattagtt
     8161 aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact tgcatgacgt
     8221 gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga atctgagcta
     8281 tcacttg
//
LOCUS       DAFBYI010000110         8227 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFBYI010000110 DAFBYI010000000
VERSION     DAFBYI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.110"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            57..251
                     /locus_tag="LLD04_002303"
     CDS             57..251
                     /locus_tag="LLD04_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649202.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            241..594
                     /gene="tnpB"
                     /locus_tag="LLD04_002304"
     CDS             241..594
                     /gene="tnpB"
                     /locus_tag="LLD04_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK5649203.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            696..2243
                     /locus_tag="LLD04_002305"
     CDS             696..2243
                     /locus_tag="LLD04_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5649204.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2415..2987)
                     /locus_tag="LLD04_002306"
     CDS             complement(2415..2987)
                     /locus_tag="LLD04_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5649205.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2994..3656)
                     /gene="rpe"
                     /locus_tag="LLD04_002307"
     CDS             complement(2994..3656)
                     /gene="rpe"
                     /locus_tag="LLD04_002307"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5649206.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3672..4187)
                     /locus_tag="LLD04_002308"
     CDS             complement(3672..4187)
                     /locus_tag="LLD04_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK5649207.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4189..4473)
                     /locus_tag="LLD04_002309"
     CDS             complement(4189..4473)
                     /locus_tag="LLD04_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649208.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4507..5835)
                     /locus_tag="LLD04_002310"
     CDS             complement(4507..5835)
                     /locus_tag="LLD04_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5649209.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(5849..6304)
                     /locus_tag="LLD04_002311"
     CDS             complement(5849..6304)
                     /locus_tag="LLD04_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5649210.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6307..>8227)
                     /locus_tag="LLD04_002312"
     CDS             complement(6307..>8227)
                     /locus_tag="LLD04_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5649211.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         2688 a         1561 c         1321 g         2657 t
ORIGIN      
        1 actaacaaat gcacctcaag atatacttga gctatcaaga agtctggagg ttttctatgt
       61 ataaattaaa tgagattgtt cctgtccaat taggaaacga aaagagtgca ccagaaaaag
      121 gtaaacccgc ggctgccaag ttagcgatgc gcatcaaaag agagaatggt tctgaagttt
      181 tgatctataa cggtattaac aattatattc ttcaagctat tttgaaagaa ctgttgtcca
      241 atgctttatg atttacttca aaccaaacat atttatattg tgtgcggaaa aacagatttg
      301 cgaaaaggaa ttgatggtct tgccagtctt attcaacaag agtatcaact cgagctttac
      361 gaagatgccg tttttttatt ttgcgggaac cgtcaagacc gtttcaaact tctctattgg
      421 gacggtgatg gttttcttct ctgttataag cggatagaaa acggcaagtt aaaatggcct
      481 cggacgaaag acgaagttcg aaccttaacg aaccaacagg ttcgatggtt gttagaagga
      541 ttaagtatcg atcaacctcg agctattttg ccaggaaaaa aaggagtttt ttaagctggg
      601 gggttaacac aaaaaatgtg ttgaacccct atttcttgtg cgtttcttgt ggtattctgt
      661 aacaaaatca gttcgattca aggtggtgaa aaattttgga aactacagat acgttgcttc
      721 agttgcttca agaagcccat aaaaccaatc aaacacaaca acaaaccatt cagaatctta
      781 caactgaaat ccaactattg aatgaaaagg tcaattattt aaccaacaaa ttatttggac
      841 gctctaaaga gactctcttt gaggaaacga atgggcaatt gaatcttttt agtgatgaag
      901 aaatttctgt aagtgttcca gaagcagctg ccaccattat tccagtaaaa ggacatcaac
      961 gagttgtagg aacgaaagca gacaaaatca aacacttacc tataacagag aaagaacatc
     1021 tccttccttt ggaagaacaa ttttgtgaac attgcggttc acagatgaaa gatatcggac
     1081 ggacaaaagt acgagaagaa attcgtttcc atcaggctat gttggattgt ctcacccatt
     1141 atcaacatac ctattgttgt aaaagctgcg aaaaagaagg actttcttct tttaaaaaag
     1201 cgatcgttcc taaacctttg atttctaata gcttaggttc caatagttta gtggcggaaa
     1261 cgatccggat gaaatttggt cagaaagttc ctgcctatcg gcaagagaac tattggaaac
     1321 aaacacatgg gttggatatt tcgagagata atatcaccaa ttggcatatt aaagcagtgc
     1381 aaaacgcgtt agacccttta ggagaacgat taagagtcta tcttaatcaa gaagagattc
     1441 tacacgggga tgaaacaagt tatcgtgtga ttgaaagtgc caaaacagat acctattact
     1501 ggcagttttg tacaggaaaa gacagtcagc atcccatcgt ttattatcac cacgacgaaa
     1561 gtcgtgctgg agatgttccg aaaacttttc tcaaggagtt tacagggtat ttacactgtg
     1621 acggctatag cgggtataat gccgtggaaa gtgtacgact tgtctattgt ttcgcgcatg
     1681 ttcggcggaa atttttcgaa gcaattccaa aaggaaagaa aaacacggat attcccgcag
     1741 ctcaagcagt caaacaattg gataaatggt ttgtcctaga aaagaaatgg aaagactttt
     1801 ctcctgagaa gcgattaagc tgtcggcaac aagaactgcg tccattattt attgcttttt
     1861 atgagtggat ggcaacgata gatcctgttg caaaatccaa gttggatgca gccgttcaat
     1921 atgcgtgtaa actacgaagt ggttttgaac ctatttttga agatggtcgt ttagaactca
     1981 caaataatcg tgcggagcga aatatcaaag agctggtgat aggacggaaa aattggttgc
     2041 attccacaag tcttgaagga gcacgaactt caggaatcat tctgagtgta tataaaacag
     2101 cagaacttaa cggattgaat cctgtaaagt atctagagtt tctatttgat aagataccaa
     2161 atcttcccgt actttcagca gaaactttag atcaactgtt gccttggaac aaggatgtac
     2221 aacaacattt ttctcgtaac taaaaacgcc aaacattccc gattctattc tataggtcgg
     2281 gttatttgac gtacaggtac tgttctatgg ggcgcttacg aattattaag ttatgtctaa
     2341 tcaaataaaa aaaccattct catatttgct gaataattag catttagaga atggtttttt
     2401 atctttaaaa tctactactc aaaatctgca tgtcgagcaa acatcgtttc tgagctttca
     2461 cctgttttat ccattaaaga taacactaaa ctatcaaagg tgagaaatgc taattgttca
     2521 aaacttgaac ccataggttg tgaaaaagta tcttcttctc gttgattttc atctttggta
     2581 atacctggta atacaagaac agaatctgct aattgaccaa tcgacgagtc ttttttcatg
     2641 gtaactaaag caatgcttac accactttga tttgctttgg ccgctaaatt ttttaaacta
     2701 cctgtttcac ctgagccaga acagataatt aataaatctc caggttgcga atgtggtgaa
     2761 ctaatttcac cgacaaggct gactgaaaaa cctaaatgca ttaagcgatt cgtaaatgct
     2821 tgcatggcaa ttccagagcg tcctgcacca gctagaaaga tgtgtttagc ttgaacaatt
     2881 ttctcactaa attgttctaa ctctacagaa ttaatacgtt ttgagttatt ttgtaattct
     2941 aagataatcg cttctaaata gtttttattt tggtctttat tggtcatgat cttttacttc
     3001 acttcctcta atgattgatt tagtttatca atttgttctt gcggggtatt tttcccattg
     3061 aagatgaaac cgcctgatac aaaaatatcc gcccctgctt tagaacaaac tttaatcgtt
     3121 tgattatcaa tcttgccatc tacttcaata tcatacgtat aatcattttg tttgcgtatt
     3181 acatctaact gttcgatttt aactactgtc tcttgaataa aatgcttatt ctctgttcct
     3241 ggattgattg tcattactaa tacttgatcg actaaagata atactggttc aataaaactg
     3301 actggtgttg caggattaaa agtaatccct acttttgctc ctgcttcctt gactaactga
     3361 atgaccttca ttgcgtgttt ggtagcttca atgtggaagg taacaatatc tgctcctgct
     3421 tctacaaatc ttgtgacaat tttttctggt gtatcaatca tcaaatgaac atcaaacaca
     3481 acgtctgtta gtggacgaat atctttaata acacttggtc caaaagccat atctggtaca
     3541 aaacagccat ccattacatc aatatgcaaa taatcaacat tcttttcttt taagatttca
     3601 aataactctt taacttctgc gataggtgca tttaacaatg atggagcaat tgtcattctc
     3661 tattcccccg tttattctcc cataatcatg agattgattg ttctagttcg ctctcttagt
     3721 tgatcccaat cgacaaaata gacacggccc atttctccta ataaaagttt accttcttga
     3781 acaattaaag tcattgagtt ggttccataa atagatgaac gtaaatgtgc atcggtattt
     3841 agcatgaccc atttttctgc agggtaattg ggatcagata atcttgttcc aaaatcaatg
     3901 tgttttggcc ccggactgtt gtattgattt tcactcgtca tttttggaat aatcgtttcc
     3961 attacattgt tgatgtctac atgtaaataa tcatcaccaa aaaagtttgt gtcatgcatc
     4021 gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg aaattactaa cattccattc
     4081 tcaatacctg ctgtatcaac tgtatctaaa agttcttttc gaatatcatg atacgaagga
     4141 cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa aacgcatatt aaacacctaa
     4201 tctttctttt acatctgcat aaatggcatc tgtaccaata cctgttaata agcccaagcc
     4261 attcgttact ttagctttaa ttacatcagg gacaattgtt gtacttacta cagcatcata
     4321 ttcatcaatt ttatttagct catcagcaat tttagattga taaattttca cttgatctgt
     4381 taatccttct tcttccaacc attttgataa ttttgctact gcaattgtag aagtagcatg
     4441 acctgctcca caaataacta ataatttttt catattgatc tcttctttct tattttaatt
     4501 ttatgattaa gctttcttca ctttgttcat gtagatagaa gcacctacta aaacaactcc
     4561 taatccacct attccaatta caccaaagta agatgctaat aaaacaaaca accctactgg
     4621 ccataatcca cataacaaca tagttactaa tcctgctgtc cccacatcga aacttgctaa
     4681 tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc atatataatg ttgtaacaac
     4741 atacaacgaa catcctacta atgatctcac aatatttcct ttaaaataag gaatcattaa
     4801 acagatggcg aaagcaaaga atgctaaatc tccaaaaggt aatactttat ttcctggaag
     4861 aattagtgct agcaataatg agattggtac aagcaataga gaagttgaca taatagttgg
     4921 atgacccaca gttaacgctg catccattcc aatgtttact tggcgaccat ttaacttttt
     4981 attcgtaaat tctttcgccg cttctgcaat tggcattaaa ccttccataa acattgctac
     5041 cattttaggc ataatcttca tcactgccgc agtcataatt cctaagtttc caattcctgc
     5101 tacgtcatag cctgccataa tacccactac aataccgata attaggccaa taacaactgt
     5161 gtcacctaaa acaccgaaac gttttgttaa tgtttctgca tctgcatcta ttttattaaa
     5221 tccaggaata cgatcaaaaa cccaatttac tggtttggca aatagtactc ctgataatcc
     5281 catcatatgt gtgatagaga ttcctggata gccgaaaaat tcgctaacag ttttttgtgt
     5341 acgatctgct aagaaccatt gtaaaattac tgcgacaatc gcgccaatta agccaaacca
     5401 aaagtttccc gtaacagatt gagtaatcat acctaggaaa attgcgaacc aaaagttcca
     5461 aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca aatatgaagt taagaattaa
     5521 tgaaattgga atcattaaaa ttcccaatga tgtagagtaa gccaaaggtc caccaacacc
     5581 tacgccaata tctaggatat tcaggccacc accgtattgt tcccccattt gttgaattgc
     5641 tgttcctaaa ccatctgata ataaacttaa aactaatcct aaaccaatag aaccgatacc
     5701 aacagtaacg ccggaaacaa atgctttacc aggttttaat ccaaataata tcccgattac
     5761 aaaaataatc agtggtaaaa agacgtttga ccctaatcca ataaaccatt caaaggcatt
     5821 taatacaata tccatctctc tattcctcct aataaactcc tgcagaatgt actaactcaa
     5881 taaatgcttc tttcgtggta catgcaagta attcatttac tttttcttca ttttgaaata
     5941 ggctaactaa gttttgtagt gtatcaattt gttcatgagg ttgtgacatt gccaacataa
     6001 agactaaacg tacaggtatt tcatgagatt tatctgtcat atctgaaaat accagtggtt
     6061 cctttaatga ggcaaaacag atttgagagc tatttacatg tgcggaatct gtatgtggga
     6121 tagcaaaccc aatttgattt acttctaatc cagttggaaa ttcttgttca cggttggtga
     6181 taccttctaa gaaactatct ttcacgcaac cagcgttttt taattgttca gacattactt
     6241 gaaacaattc tttttgatct ttcacttcta attgaaataa acataattct ttatctagat
     6301 acatttttat tctccttttt gttgaaaaat tcttagtaat ttttcgctat catgaacttg
     6361 taacaagcga tcgaccattt ctttttgccc aataaattgg taaagttctt catatactcc
     6421 tctaatctca gatacatctt cttcagctaa actaaccata atgactaatt tgacttttcg
     6481 atcgccccaa ttaatcgcat tttttaatgc tacaataaaa atgttggatt caatgaccat
     6541 tgatggattc gcatgcggta aagcaacacc actttctaaa ttagtattcc ccattttctc
     6601 tctagagaag atagaagcac gaaactcttc agtaactaat gaattggctt ctagttcgga
     6661 aatcatcaaa tccaaacatt cttctttgct ttccacctgt actcctgttt ttactaaaga
     6721 tgattgaaca aatttagata tggtgggaac tttaaccgct ttaggaatca gttcattttg
     6781 aataaatcgc gaaggttctt ctattaaata tttagtataa gcttccataa tattcatata
     6841 atctgtttta cttaccaatg gagataccct tactaagggt ataccacctg catcaaagga
     6901 aagagaggaa ataatcaaat caacttcccc aatttcagct aaatgaaact tatctaaagt
     6961 tgatatttct acattatctt ttgctggtaa aaaccgtcga actttattgt aaattaattg
     7021 cgcagaagaa ataccgtatt ggcaaataat gatgatatta tttgctttgg actgatcttc
     7081 aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca tcgtccgtta aagtcacatc
     7141 aaattcttct tcaaggatgg acatggcgta ccatactatg ctaaagagtt ctgaatattg
     7201 agatttgata ttttctaata acggattttg aattttaata ccttttttta aacgaaggac
     7261 cattgctggc acatgataca ataaggattg atataatcga tcattttgag tcaaatcaat
     7321 tttttcaatc tcactcattc tttcaatcaa tttatgaacg ctcatagcgt actcttcatt
     7381 gctggttttt aaattatttg tcacacgatg tgcaaatagt tgacgacata gatattcttt
     7441 atctgcgtca taaaatggaa gattcaattt tgccttcaat tcttcaatta aaccatttgc
     7501 cacaatgtac gtttccatat agcgaatgct attgaagagg aattcttctt caacagttaa
     7561 atgatgacct ttcagtaaac ggttacattg gataactaaa gtagaaatca atgaccgaac
     7621 atagtaatca gaaacctttt cagtcagttc actgtaatca ttgaatagca aatcatgaat
     7681 gacactaata acttttgaat caaacaacat atttaaaact tgaaaaaatg tttttgattc
     7741 catgtcattc gcataataga atacaagttg cttgattgct cgttgaattg cttgttcatt
     7801 accagtaaaa ctaattcctt ctgcagatga ctgcaactta acttctccac tgccaataat
     7861 tttattgata attctcaggt cattgtataa cgatgtttta ctaaccataa aactttgaga
     7921 caaatgagtt aaagaaacag gcttatcacc aaacaaaact tctttaatga tctcgacacg
     7981 tcttttttct ggagaataaa tatctttttc aagataattt tctaccgatg aaatcaaatg
     8041 ttcaacgttt ttcctatcac ctatgagttt gatacccaaa gaaggtaatc gctcaattgt
     8101 tacttggtaa ttaactacat aatcttccaa caattcaata tcttgataaa tggtcttggt
     8161 cgacactgcc aatttatcac taaaaaaaga agcgggttta aagtcttctt gtcgacttaa
     8221 taatttt
//
LOCUS       DAFBYI010000111         7866 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFBYI010000111 DAFBYI010000000
VERSION     DAFBYI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.111"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(52..1392)
                     /locus_tag="LLD04_002313"
     CDS             complement(52..1392)
                     /locus_tag="LLD04_002313"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK5649212.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(1629..3152)
                     /locus_tag="LLD04_002314"
     CDS             complement(1629..3152)
                     /locus_tag="LLD04_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK5649213.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(3530..4141)
                     /locus_tag="LLD04_002315"
     CDS             complement(3530..4141)
                     /locus_tag="LLD04_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5649214.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(4264..4389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4471..5187)
                     /locus_tag="LLD04_002316"
     CDS             complement(4471..5187)
                     /locus_tag="LLD04_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5649215.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(5193..5762)
                     /gene="scpB"
                     /locus_tag="LLD04_002317"
     CDS             complement(5193..5762)
                     /gene="scpB"
                     /locus_tag="LLD04_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK5649216.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(5746..6543)
                     /locus_tag="LLD04_002318"
     CDS             complement(5746..6543)
                     /locus_tag="LLD04_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK5649217.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(6513..6884)
                     /locus_tag="LLD04_002319"
     CDS             complement(6513..6884)
                     /locus_tag="LLD04_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5649218.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(6903..7790)
                     /gene="xerD"
                     /locus_tag="LLD04_002320"
     CDS             complement(6903..7790)
                     /gene="xerD"
                     /locus_tag="LLD04_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK5649219.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         2244 a         1710 c         1328 g         2584 t
ORIGIN      
        1 aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc
       61 acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc
      121 ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc
      181 cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat
      241 gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg
      301 cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt
      361 tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac
      421 accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc
      481 ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa
      541 ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg
      601 agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac
      661 gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc
      721 agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc
      781 ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc
      841 tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt
      901 ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa agataaaacg
      961 aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc
     1021 aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt
     1081 tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg
     1141 acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc
     1201 tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc
     1261 ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga
     1321 aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac
     1381 aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg
     1441 acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg
     1501 ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac ttgtccacgt
     1561 gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt
     1621 gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac aaataatatc
     1681 gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca
     1741 gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga
     1801 taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt
     1861 cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag
     1921 aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc
     1981 caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca
     2041 acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca
     2101 aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac
     2161 atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta
     2221 cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca
     2281 ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat
     2341 acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg
     2401 aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg
     2461 atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc
     2521 aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct
     2581 gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt
     2641 gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag
     2701 aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc
     2761 aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg
     2821 gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag
     2881 aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc
     2941 atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt
     3001 tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata
     3061 aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc
     3121 atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag
     3181 acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac
     3241 acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt
     3301 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
     3361 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
     3421 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
     3481 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
     3541 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
     3601 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
     3661 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
     3721 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
     3781 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
     3841 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
     3901 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
     3961 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
     4021 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
     4081 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
     4141 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
     4201 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
     4261 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
     4321 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
     4381 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
     4441 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
     4501 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
     4561 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
     4621 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
     4681 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
     4741 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
     4801 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
     4861 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
     4921 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
     4981 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
     5041 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
     5101 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
     5161 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
     5221 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
     5281 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
     5341 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
     5401 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
     5461 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
     5521 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
     5581 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
     5641 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
     5701 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
     5761 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
     5821 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
     5881 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
     5941 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
     6001 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
     6061 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
     6121 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
     6181 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
     6241 ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca ggaagcaatg tcttgctttt
     6301 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
     6361 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
     6421 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
     6481 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
     6541 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
     6601 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
     6661 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
     6721 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
     6781 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
     6841 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
     6901 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
     6961 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
     7021 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
     7081 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
     7141 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
     7201 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
     7261 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
     7321 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
     7381 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
     7441 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
     7501 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
     7561 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
     7621 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
     7681 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
     7741 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
     7801 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
     7861 agcaaa
//
LOCUS       DAFBYI010000112         7854 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFBYI010000112 DAFBYI010000000
VERSION     DAFBYI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.112"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(110..1120)
                     /gene="trpS"
                     /locus_tag="LLD04_002321"
     CDS             complement(110..1120)
                     /gene="trpS"
                     /locus_tag="LLD04_002321"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK5649220.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            1374..2111
                     /locus_tag="LLD04_002322"
     CDS             1374..2111
                     /locus_tag="LLD04_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5649221.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            2125..2940
                     /locus_tag="LLD04_002323"
     CDS             2125..2940
                     /locus_tag="LLD04_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5649222.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            2953..3600
                     /locus_tag="LLD04_002324"
     CDS             2953..3600
                     /locus_tag="LLD04_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5649223.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            3616..4272
                     /locus_tag="LLD04_002325"
     CDS             3616..4272
                     /locus_tag="LLD04_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5649224.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            4434..5255
                     /gene="racE"
                     /locus_tag="LLD04_002326"
     CDS             4434..5255
                     /gene="racE"
                     /locus_tag="LLD04_002326"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK5649225.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            5258..6613
                     /gene="rph"
                     /locus_tag="LLD04_002327"
     CDS             5258..6613
                     /gene="rph"
                     /locus_tag="LLD04_002327"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK5649226.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            6614..7132
                     /locus_tag="LLD04_002328"
     CDS             6614..7132
                     /locus_tag="LLD04_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5649227.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            7239..7739
                     /locus_tag="LLD04_002329"
     CDS             7239..7739
                     /locus_tag="LLD04_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5649228.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2541 a         1358 c         1682 g         2273 t
ORIGIN      
        1 gatgtgacac aacttcgtca catctctttt tttgatttta aaaaaggaat agcacagaga
       61 cgagataaaa aaacaggcct ttacagcctg cttttttaga gtaaatgaat taatggaagt
      121 aattgattcc cattgctgat tttacttcag aaagtgtttg tgcagctact ttagcagctt
      181 cttcactacc tttacgtaag atttccataa cagctgaagg atctttttcc aattcttctc
      241 ggcgcgcacg gatcggtgca aattcagctt ccaatacatc aatgaggtat cgtttgatct
      301 ttacatcacc aagtccgccg cggcggtaat gagctttcat ttcttcaatt gcttctttat
      361 ctgttccgaa tacatccaag taggtgaaga ccatattccc ttctacttgt ccaggatctt
      421 gtacatggat gtggtttgga tcagtataca tactcatgac ttttttctgt aagacatcgg
      481 caggatcgga gatataaata ccgtttccta acgatttact cattttgcca tttccatcga
      541 ttccaggtaa tctacccatt ccttttggtg ggaatacgcc ttttggttcc accaagactt
      601 caccatatgt atgattgaag ctttgtacga tttcctgtgt ctgctctagc attggttttt
      661 gatcctcacc aacaggtaca agattcgctt tgaatgcagt gatatctgct gcttgagaaa
      721 caggatagat aaagaatcct gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg
      781 ttttgacagt cggattgcgc cgaacgcgtc ctacgctgac taagttcaaa taatacatgg
      841 ttaattcagc taattccgga atttgtgatt ggataaataa agtagatttt gcaggatcta
      901 atcccacggc taaatagtct aaagcaactt gcaagacatt tgatgaaacc ttctcaggat
      961 ttttagcatt atctgtcagt gcttgcatgt cagcaatcat gacaaacagt tggttgtctt
     1021 catctgcctg catagccact cgggttttta gagaaccgac gtaatgccct aaatgcagct
     1081 tcccagttgg tcgatctccc gttaaaataa tatttttcaa aaaagacacc tctttacgat
     1141 ttgtaagtct gagcacttaa tatcttaccg tttttcctga aaagaaacaa cagttcctaa
     1201 ggaaaaaaca aggaaagagg ataaaataac taatagatta gaaatggaag aaatatttat
     1261 gggagaataa acgttctaag aaaatttaca aattactaaa ctatcagata taatgtattt
     1321 tattatacat ctttaaaaga aatcttacta aataataatg ggagtgtaat tctatggcaa
     1381 tgattaaatt tgatcacgtg gataaatatt atggcaagtt ccacgcatta aaaaatatca
     1441 atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga
     1501 gcacgatgct tcgctgtatc aatggattag agacgatctc ttcaggtaag ctgctgatta
     1561 atgatactga cttgcatgat aaaaagacaa aactgacaga agtccgaaaa aacatcggca
     1621 tggttttcca acatttcaat ttgtacccaa ataaaactgt tctggaaaac atcaaattag
     1681 caccaatcaa agtgttgaag caagaccagg caactgcagt caaaaacgcg gagaaatttt
     1741 tgaaaactgt caatatgtta gataagaaag attcttatcc ttccatgtta tccgggggac
     1801 aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg
     1861 atgaacctac ttctgctttg gatccggaaa tgattggtga tgtattagac gttatgaaaa
     1921 aattggctca tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag
     1981 aagtggcgga tcgtgttatc ttcatggctg atggagaagt attggaagac agcagaaatg
     2041 tgagagactt tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc agtaaagtta
     2101 tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct gattttcctt
     2161 ttaggcattg cttttcttgc actagccgga tgtaaaggaa atgggttggc aaatgaagat
     2221 attttagagc gaagcaaatc taccaatgaa atcatctggg gtgtcaagta tgatactcga
     2281 ttatttggaa tgatggatat tgagagcaga actgtacaag gatttgatgt agatattgca
     2341 aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt tgaagtcact
     2401 tctaaaactc ggattccatt attgaaaaac ggaaatatcg atgcaattat tgcaacgatg
     2461 acgatcacag atgaacgaaa aaaacaggtt gacttttcag atgtctattt cgatgctggc
     2521 caagctttgt tggtaaagaa aggaagccaa atcaaaagtg tagatgattt gaatgcttct
     2581 acaacggttc tagcagtcaa aggttctaca tcggctgcga atatccgaca gcatgcacca
     2641 gatgcaaaaa ttttagaatt agaaaactat gcagaagcat ttactgcttt acaatctggt
     2701 cagggagacg cgatgactac ggataatgcg attttgttag ggattgcaga tgaaaatcca
     2761 gagtatgagt tagttggtgg aacgtttact aatgagcctt acgggattgc gatcaacaaa
     2821 gggcaagaaa acttcttgaa agctgttaac caagcgctag aggaaatgca tgcagatggc
     2881 acatacgata aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa
     2941 gggggagagg caatgagcga attgatacaa acttatggac caatctttgt cgacgggttc
     3001 aagatcacat tgttttcgag tgtcttagca cttgtgttta gtttgattat cggaacgtta
     3061 atggcaatct tccaattatc aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta
     3121 gaatttttta gaaacattcc attattaatc atcgtgatgt ttttttatgt tgtcgtaccg
     3181 atttattggt ttaatctaga tggattccaa gcaggtacga ttggattaac gatctataca
     3241 tctgctttta ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca
     3301 gaagcaggac tatcttctgg ttttacctat tcagaaatca tgcgttatat tgttttaccg
     3361 caagcattca aaatcgttat cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac
     3421 tcatctattt tagcaatcgt agctggttta gatttgatgt atcaaggaga cttgatcgct
     3481 agtgaaacat tcaatacctt cgatacgtat atcatcgttg ggttatttta cttagttatc
     3541 acgctaccgt tatcttactt aatggtttat cttgagaaaa aatgggcggt tcgttcatag
     3601 aaggagggga aatacatgga ttttagcggc gctttttcat ggatgaatat ccgtttttta
     3661 ttggaaggat taaaggttac tgttgaagtt tcattgtttt cgatcatttt tagtttcttg
     3721 atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc caaaatcctt
     3781 ggtgtagtga ttgatattat acggaatctt ccattattat tgatcatttt cttcacttac
     3841 tttgccttac ctcaaatcgg cattcagatg aacatcttct ggtccgctgt cgcagcatta
     3901 acgatttttg agtcagctat gttgtcggaa attttccgag ctggactcaa tgctgttcca
     3961 aaaggacaga tggaagcagg attatcaacg ggactgactt atgtagaatc gatgcgaacg
     4021 atcgtgttac cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca attgatttca
     4081 ctgatcaaag atacttcatt agcagtaatc atttctttac ctgaactttc tcatcaggca
     4141 cgtattattt atggacagaa caccaattat gtgcttccga tgttcatcat catgacactt
     4201 atgtacttcg tggtatgtta cgcgctttct ctgctttccg gttacttgga aaagagacgg
     4261 tacagttatt aatagatgat tcttttagcg gttgtggcaa actaaagtca caaccgcttt
     4321 tcctctttcc attcgaaaaa aagagatttc gattgtttta agcaagtaag agcacttgaa
     4381 aattcacgct aaaaagtaca caatagggag gatagaattt attgaaaaaa aggatgatac
     4441 gattgacaga taatcgccct atcggattta ttgattcagg tgtcggcggc ttgactgtag
     4501 taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt atttgtagga gacacagcac
     4561 gctgcccata tggccctaga cccgcggaac aggtaatcca gtatacttgg gaaatgacgg
     4621 attatctggt ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg
     4681 tggctttaga agaaatcaaa gctgctcttt ctattccagt catcggtgtg atccttcccg
     4741 gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc ggtacgattg
     4801 gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga gaaagtacca gaattgactg
     4861 tgacaagtct tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa taccattcat
     4921 cggtggcgaa aaaaattgtg gcagaaacat tagctccttt aaccactaaa aaaatcgata
     4981 cattgatttt gggatgcacc cattatccat tattacgccc catcattcaa aatgtaatgg
     5041 gagaaaatgt tcaactgatc gattctggag cagaaacagt aggtgaagta tctatgctgt
     5101 tagattattt caatttgagc aattcaccgc aaaatggtcg gacattatgc cagttttata
     5161 caactggctc tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga atcggacact
     5221 taaatgtaga acatatcgaa ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc
     5281 ccaagagctt cgcaaactgg agcttcaaac aaatgttttc aaatatccag aaggatcggt
     5341 agtgatccgt tttggtgata caacagttat ttgttctgca acaatagaaa attctgttcc
     5401 cccttttttg agagaaacag gaacaggatg ggtgacagca gaatatagta tgctgcctcg
     5461 agcaacgagt acgagaaatc gtagagaaag cagcaaaggg aaactttctg gtagaacaat
     5521 ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg
     5581 agaacgtagt atcatcgtag actgcgatgt gatccaagcg gatggcggta ctagaacagc
     5641 aagtatcact ggggcatttg tggcattgaa attagccatt gaaaaacttt taagagaaaa
     5701 agaattgtca gaagatccaa taaaagaaca tcttgctgca gttagcgtag gaatcttacc
     5761 agatgggact tgcgtcacag atttggatta ccaagaagat tccgcagcac ttgtagacat
     5821 gaatcttgta atgacagaat ctggaaaatt cgtggagatt caaggaactg gagaagaagc
     5881 aacgtttgat ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga
     5941 tttgatcaaa gagcaaaaac atgctttgct aacagagttt gctcaaaatg atgagcggat
     6001 agaagaaaca aaaacaatca tcattgcgac aagaaatcca ggaaaagcag aagagtttcg
     6061 aaatatgttc aaagaagcgg gatatcatgt aaaaacatta cttgactatc ctgaattgcc
     6121 agatgtggaa gaaactggca gcacatttga agaaaatgcc cgattaaaag cagaaacgat
     6181 tgcacaattg cttgatcagc cagtcttagc agatgattca ggtttgaaag tggatgcttt
     6241 aggcggtatg ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg
     6301 gaataacgcc aaattattat atgaattgac agatgtgccg gatgaaaaaa gaacagcaca
     6361 attccattgt acgttagtat tcgcggcacc caaaaaagac agccttgtag tagaagcgga
     6421 atggcccggt cgagtagccc gtataccatc tggcgaaaat ggctttgggt atgatccttt
     6481 atttatccca gaaggtaaga aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa
     6541 gatcagtcat cgggctcaag caatgaaaaa attgagtgcc gagtggaaac aatggctgga
     6601 aggagagaga tagatgcggt atttagtagt aagtgataat catggagaca gagatatcct
     6661 cgttgactta gtagatcgtt atcgttctga agtggatatc tttttccatt gtggtgattc
     6721 ggaattagaa gcttctgacc cattatggga tacattccaa gtggttcaag gtaattgtga
     6781 ttatggacca ggattcgaac agaaaaaagt gatccaaact ggtcaggata cagtattcat
     6841 gactcacggt catttatcga atgtccgatt tggtttgaca cagttagcaa tcgaagctcg
     6901 agctgcaact gccgatatgg tgttttttgg tcatacccat caaataggct gtgagatgga
     6961 tcagaaaatc ctgtttttga atcctggtag catttctcag cctagaggcc cgatccaaat
     7021 cccatcatat gctcttatcg aaagtttaga tgaccaaatt tctgttcaat actataaccg
     7081 tgcccatcag gcagttgaag agttggcctt ttctttttcg agagagcgct gaataaaaaa
     7141 actctaattt ttaataaaaa taagctttat tttatgaatt ttgcatccgg tttcggtaaa
     7201 atagtagtgt tgaatgaaat agaaatggag gccatcagat gattggacct atagtaagag
     7261 aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta
     7321 tgtatcatca tccattgtcc catggtttac ttgtgttatc gaaggtcggt tattccaaaa
     7381 ttcctgtatt aggaaaagat gatcactttg taggactagt aagtttatca gatgtggtca
     7441 ataaaatgat tgacttgcaa acaatcagca tggacccgtt agaaggacta acggttgctg
     7501 atgtgacaga aacagatgtt ccgacaattg atgaaaattg ggaactagaa gaagtactcc
     7561 atttgttagt cgattcgcct tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa
     7621 tcattacaag aaaagagctg cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc
     7681 gtaatatatt gtctccgaaa atcgaagaat taaaagaaca aatcgatatc gtgagctaaa
     7741 gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac cagttttgac
     7801 tttatcttga aaaagaggct gggacaaaag cgtttaactc caagaaataa gaag
//
LOCUS       DAFBYI010000113         7775 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFBYI010000113 DAFBYI010000000
VERSION     DAFBYI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.113"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(178..2070)
                     /locus_tag="LLD04_002330"
     CDS             complement(178..2070)
                     /locus_tag="LLD04_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649229.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="LLD04_002331"
     CDS             complement(2067..3794)
                     /locus_tag="LLD04_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649230.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="LLD04_002332"
     CDS             complement(3810..4460)
                     /locus_tag="LLD04_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5649231.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="LLD04_002333"
                     /pseudo
     CDS             4664..4960
                     /locus_tag="LLD04_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5132..5752
                     /locus_tag="LLD04_002334"
     CDS             5132..5752
                     /locus_tag="LLD04_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK5649232.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="LLD04_002335"
     CDS             complement(5844..7010)
                     /locus_tag="LLD04_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5649233.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="LLD04_002336"
     CDS             complement(7022..7375)
                     /locus_tag="LLD04_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5649234.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..>7775
                     /locus_tag="LLD04_002337"
     CDS             7503..>7775
                     /locus_tag="LLD04_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HBK5649235.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVD"
BASE COUNT         2332 a         1610 c         1394 g         2439 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttaa tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgat
//
LOCUS       DAFBYI010000114         7761 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFBYI010000114 DAFBYI010000000
VERSION     DAFBYI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.114"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            147..1094
                     /locus_tag="LLD04_002338"
     CDS             147..1094
                     /locus_tag="LLD04_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5649236.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="LLD04_002339"
     CDS             1106..2026
                     /locus_tag="LLD04_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5649237.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="LLD04_002340"
     CDS             2067..3524
                     /locus_tag="LLD04_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5649238.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="LLD04_002341"
     CDS             3869..4483
                     /locus_tag="LLD04_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5649239.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="LLD04_002342"
     CDS             4476..6200
                     /locus_tag="LLD04_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5649240.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="LLD04_002343"
     CDS             6197..7648
                     /locus_tag="LLD04_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5649241.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1291 c         1543 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DAFBYI010000115         7718 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFBYI010000115 DAFBYI010000000
VERSION     DAFBYI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.115"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            143..520
                     /locus_tag="LLD04_002344"
     CDS             143..520
                     /locus_tag="LLD04_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5649242.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            508..846
                     /locus_tag="LLD04_002345"
     CDS             508..846
                     /locus_tag="LLD04_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5649243.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            980..1423
                     /locus_tag="LLD04_002346"
     CDS             980..1423
                     /locus_tag="LLD04_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK5649244.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1685..2500
                     /locus_tag="LLD04_002347"
     CDS             1685..2500
                     /locus_tag="LLD04_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5649245.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2510..3172
                     /locus_tag="LLD04_002348"
     CDS             2510..3172
                     /locus_tag="LLD04_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5649246.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3377..5041
                     /gene="alsS"
                     /locus_tag="LLD04_002349"
     CDS             3377..5041
                     /gene="alsS"
                     /locus_tag="LLD04_002349"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK5649247.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5054..5764
                     /gene="budA"
                     /locus_tag="LLD04_002350"
     CDS             5054..5764
                     /gene="budA"
                     /locus_tag="LLD04_002350"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK5649248.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5894..6391
                     /locus_tag="LLD04_002351"
     CDS             5894..6391
                     /locus_tag="LLD04_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK5649249.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            6576..6836
                     /locus_tag="LLD04_002352"
     CDS             6576..6836
                     /locus_tag="LLD04_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649250.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7198..7437)
                     /locus_tag="LLD04_002353"
     CDS             complement(7198..7437)
                     /locus_tag="LLD04_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649251.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         2569 a         1183 c         1599 g         2367 t
ORIGIN      
        1 tgaaataata cacaaacaac cgatatagat aatccagctt agttttaata gaaatgagta
       61 ttgacttgga gtacacttaa agttgtagaa taaataaaat acataagtaa agtggcaaga
      121 aattaagaga aagagtgatc agatgaatat caaacaagtc agtgaagaaa agggcatttc
      181 ggccgataca ttgcgctatt atgaacgaat cgggttgatt ccaccagtta atcggacaaa
      241 aggcggaatc cgtgattata cagaagaaga cctaaaatgg gtcgacttta cgatctgtat
      301 gcgaaatgcc ggattatcga tcgagtcttt gattgaatat attcgcctat ctagcgaagg
      361 agaagcaact gtatttgagc ggcgtaagct tttgattgaa gaaagtgaac ttttgacgaa
      421 aaaaattgct gaaatgcaag aatgtttgga acgtttacaa ggaaaaatca agaaatatga
      481 tcgtgtgtta tctagaaata aaatggaatg tatcaagtaa aaaaatcaag gagcatcgct
      541 atggagttac gcttactaaa atatttttgg acaattgcag aagaaggaac aatctctcaa
      601 gccgcagaag ttctacatct tacacaacca accttaagcc gacaactgaa ggagttagaa
      661 gaggaactag gaacagaact gtttgtacgc gaaaaccgca aaatgatctt gacagaagca
      721 ggttattttc taaaaaagcc gagcagaaga aattttagat ttaacgaatc aaaccactca
      781 agaattcgag aacagaaaaa aacaattatt cagcggccga tttacgatcg gctgtgtgga
      841 agctgataat tcgaatacat tggcactatt actagaaaag ttgatccgtg attatcccag
      901 cgttcaattt catatccaca gcggaacaag tgatgatatc acggagtgac tagaaaaaag
      961 cctgttagat ttagcgattt tgctagatcc agtagataca gagaaatacg aaacgatttt
     1021 gttaccaaga aaggaaaaat ggggattgct cattttagaa aattctgttt tagcaaaaaa
     1081 agacgtgata acaaaagaag actttgcgaa tgtacctctt ttgatctcaa agcgtccaga
     1141 aatacagcga atgtttgctg actggttagg cataaaaaag gagcagttgc tgatcacagg
     1201 aacgtacaat ttgattttca atgtatttgc gctaattgag aatcaggtag gtgctgcttt
     1261 agtcatagaa ggtgctgtaa gtcatctttc aaagcgagtg acattcgttc cgcttgaccc
     1321 aatgattgaa acgaactgca tgctagtctg gaaaaagaat cggattttat cacctgtagt
     1381 caatgaattg attcaccgtt tcaaagaagc ttttgaacag taataagtat ctctaatcga
     1441 agaaatttca atacaaaagc gagacgtatg acagtcttgc ttttgtattt tttcattttt
     1501 aaatcaaaaa aaataaagag atttacataa cttgttaaat aagcgcttac aaacataaga
     1561 agaacggaaa tgaatcagct ataataaccg ttgttctgga ttgtaatcat tacaaagaaa
     1621 tgaagattat aactttggag gtgttcgaaa tgaaagaaaa aaagcaaaaa ttcgttcaaa
     1681 aaacatgccc tttattcaat ggttgggtat gattctgctg attgtattag ctttattgac
     1741 gatcattcct tttttatgga tggtttctgc ttcattcaaa gcaaataatg aagtgttcac
     1801 gattccgatt caatggatcc ctaaaacttg gcatttagag aattatctgg ttatttggtc
     1861 tcgtattcct ttagtgacat tttttaagaa tacagctttt ttaagcgttg tgatcacgtt
     1921 gattcagttg tttacttcta gttttgctgc ttacgggttt tctaaactaa attttaaagg
     1981 gcgcgatatt ttatttttag catatatcgg cacgattgct gtcccttggc agtcttatat
     2041 gatcccgcag ttcattatga tgagaaaagt gggtttaaca tatacgttgt ggtcgttggt
     2101 gctattacaa gcgttcaatg cttttggtgt gttcttgttg aagcaatact acagtagtat
     2161 cccagattct ttatgtgaga gtgcgcggat cgatggctta tcagagtggg gaatctatcg
     2221 caagatcatt ttacctctta caaaaccggc gattgccagt ttgacgatca tcacattcgt
     2281 taatacatgg aacgattata tggggccatt catttatttg tcttctacgg aaaataaaac
     2341 aatccagtta gggttgaaaa tgtttattgg gctatttgat gcagagtacg cattaatcat
     2401 ggctgcctca gttgtttctg ttcttcctgt tgcttttata tttttagcga tgcaaaaata
     2461 ttttgtagaa gggatcgctt catcaggaat gaaggaatag gaggtgatta tgaaagagca
     2521 gcgttttgac aaaaatgttt atatggaatt cttttattgg ttgtatcaat cattggcaag
     2581 tagcctttgt ttttggtgtg tcaatatctt gtatatagca gctgcgttcc ttttgtcgat
     2641 agatggaagg aatgtctttt cctttatttt ttctttactt tttattggtc caggttgcat
     2701 cgtgatgata ggtatgacag aagaattagc aaaagaaaaa agtttttcac cagtacgttt
     2761 gttcataaag acgtttcgcc aattttggct aaagggattt ttttattggc tatttgcttg
     2821 gatagttagt gtgataatga tttttgattg tttctttttt atacgttttt cttatggaaa
     2881 atggttgatt ccgttgtttg ttttacttgc ttgtttatca gtttccttta gcatcaacta
     2941 ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac ggatagcgtt
     3001 ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat tgacaagttt
     3061 gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt ctatcgtatt
     3121 tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt agactttata
     3181 aattgaaact ttcaattaag aatgacagtt tttatgtaaa aactgtcatt ctttttcatt
     3241 ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct atacttttga atgaaacagg
     3301 tttagaaact gtatcaatta tttgtgatta tttctaacta ataattaaaa ataaagcata
     3361 aaaaggacga tgttcaatgg acgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag
     3421 tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt
     3481 atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc
     3541 tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac
     3601 aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga
     3661 cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca
     3721 aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga
     3781 tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa
     3841 aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aagggaatgt
     3901 catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct
     3961 agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag
     4021 tgaaaaagaa acgttagcca tcaggcaatt agttggaaag acagcattgc ctgttgtaga
     4081 gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt
     4141 gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc
     4201 tattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg
     4261 gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga
     4321 attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca
     4381 agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcagaattaa cggagcggga
     4441 cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct
     4501 tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg
     4561 gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca
     4621 aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca
     4681 aatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc
     4741 ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt
     4801 agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga
     4861 ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga
     4921 attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc
     4981 gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta
     5041 aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat
     5101 ggctggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg
     5161 attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca
     5221 aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc
     5281 aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca
     5341 attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat
     5401 aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag
     5461 aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg tccaaggaac
     5521 attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcactt
     5581 gcattttatc gatgatacac atacatttgg cggacatgta atggattttg aagtggctga
     5641 aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc
     5701 gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga
     5761 atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga
     5821 ggaggaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg
     5881 agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt
     5941 acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc
     6001 tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg
     6061 taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg
     6121 aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa
     6181 aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat
     6241 ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa cgaaagggta
     6301 tgatgaaatc gatcaagcat ttctagaaaa atatgtagat cttttattag caaattttaa
     6361 tataaatgct ttttcattta ttaataacta aaatcgtcta ttgaaacaaa aaagaagaga
     6421 aatcaagaat aaaaagagcc ggaaagacag tgggcatttc tgtttttcgg tttttttatt
     6481 tttttataaa atcacaagtg ttattgattt tcataatcat attttaatgg tattatcatt
     6541 ttaaataatg gaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag
     6601 gaaaaaaata tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca
     6661 gtaaattaga aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg
     6721 aaatattaga aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa
     6781 caacagaata tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat
     6841 ttaaataaag tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg
     6901 gcagtgattg ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg
     6961 taacgaggcc tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc
     7021 ggatagacaa actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa
     7081 aactgatatc aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca
     7141 tttagttagg agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta
     7201 attcagagtt tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc
     7261 agaggtagaa acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat
     7321 tgttgaatcg atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt
     7381 cccacaagca gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat
     7441 gtacacactc cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa
     7501 tgcaaaacta tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg
     7561 gtgatatccc agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct
     7621 aaacgctttt tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg
     7681 taattttttt gggtatttaa atagttgcta ggaggata
//
LOCUS       DAFBYI010000116         7652 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFBYI010000116 DAFBYI010000000
VERSION     DAFBYI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.116"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(5..613)
                     /locus_tag="LLD04_002354"
     CDS             complement(5..613)
                     /locus_tag="LLD04_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649252.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(772..2169)
                     /locus_tag="LLD04_002355"
     CDS             complement(772..2169)
                     /locus_tag="LLD04_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5649253.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKYYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2201..3172)
                     /locus_tag="LLD04_002356"
     CDS             complement(2201..3172)
                     /locus_tag="LLD04_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5649254.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3546..3971
                     /locus_tag="LLD04_002357"
     CDS             3546..3971
                     /locus_tag="LLD04_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649255.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4459..5160)
                     /locus_tag="LLD04_002358"
     CDS             complement(4459..5160)
                     /locus_tag="LLD04_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5649256.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5176..5616)
                     /locus_tag="LLD04_002359"
     CDS             complement(5176..5616)
                     /locus_tag="LLD04_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5649257.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5616..6191)
                     /locus_tag="LLD04_002360"
     CDS             complement(5616..6191)
                     /locus_tag="LLD04_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK5649258.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7039..7518)
                     /locus_tag="LLD04_002361"
                     /pseudo
     CDS             complement(7039..7518)
                     /locus_tag="LLD04_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2189 a         1584 c         1226 g         2653 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt
      301 taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat
      361 tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta
      421 ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt
      481 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
      541 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
      601 tttttttcct catgctttcc tccactccta tttaattttg actattctat tatatacaaa
      661 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca
      721 caaaatggta agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc
      781 aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt
      841 tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca
      901 cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat
      961 ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat catcgtggat
     1021 catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat
     1081 aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg
     1141 ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc
     1201 gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac
     1261 ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt
     1321 caaatcctct gcatggatca caggcatgag atcaagttct tttactatct caatcaattc
     1381 acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc
     1441 tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag
     1501 attcaagatg ataccaataa caccaccttg ctccatttca tggtaatatt tgatggctaa
     1561 cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc
     1621 tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt
     1681 aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata
     1741 ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc
     1801 gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat caaaataacg
     1861 atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct
     1921 ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc
     1981 ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg
     2041 cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct
     2101 tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata
     2161 ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga
     2221 tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca
     2281 gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa
     2341 ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga
     2401 ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga
     2461 caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc
     2521 tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt
     2581 tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga
     2641 tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat
     2701 tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa
     2761 tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca
     2821 gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat
     2881 cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg
     2941 agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc
     3001 ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg
     3061 cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa
     3121 ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc
     3181 actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct
     3241 ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc
     3301 ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt
     3361 tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct
     3421 ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc
     3481 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
     3541 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
     3601 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
     3661 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
     3721 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
     3781 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
     3841 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
     3901 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
     3961 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
     4021 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
     4081 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
     4141 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
     4201 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
     4261 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
     4321 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
     4381 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg
     4441 tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa
     4501 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac
     4561 cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt
     4621 aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata
     4681 ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg
     4741 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg
     4801 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
     4861 gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat
     4921 ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
     4981 gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg
     5041 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
     5101 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
     5161 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa
     5221 atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat
     5281 caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc
     5341 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
     5401 aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca
     5461 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
     5521 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
     5581 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
     5641 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
     5701 attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
     5761 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc
     5821 agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca
     5881 gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt
     5941 tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc
     6001 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
     6061 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct
     6121 aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca
     6181 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
     6241 ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata gacgccacta
     6301 tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac aaaaataaat
     6361 aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat ttcgtcctat
     6421 atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt aacgaaaaaa
     6481 tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat aaaaaagccg
     6541 acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta aaaatctttt
     6601 tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact gctggaagaa
     6661 aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat attgggctcg
     6721 aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata ataactttct
     6781 ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac ataaggtata
     6841 ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt caaggagggt
     6901 gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt cgattctttc
     6961 taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac ttttttcttg
     7021 taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt cttcttcctc
     7081 tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata ataaaatggt
     7141 tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg gtctggtcat
     7201 cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc aaaacatgct
     7261 taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg ccaagtcgtt
     7321 caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa aagcggtctt
     7381 caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc ctaagaaata
     7441 agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag tgattggttc
     7501 atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg taatccagaa
     7561 acagccgtgt atctgcggta ataagcctaa ccaaacgaaa aatatgagaa gctatttttg
     7621 tttggctgtt cttattactt gatgcagggc ag
//
LOCUS       DAFBYI010000117         7638 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFBYI010000117 DAFBYI010000000
VERSION     DAFBYI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.117"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..920
                     /locus_tag="LLD04_002362"
     CDS             <1..920
                     /locus_tag="LLD04_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5649259.1"
                     /translation="GSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLD
                     MLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKR
                     QETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVIL
                     DQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTG
                     IGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQ
                     IKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            1111..1602
                     /locus_tag="LLD04_002363"
     CDS             1111..1602
                     /locus_tag="LLD04_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649260.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            1705..2694
                     /gene="manX"
                     /locus_tag="LLD04_002364"
     CDS             1705..2694
                     /gene="manX"
                     /locus_tag="LLD04_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK5649261.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            2727..3530
                     /locus_tag="LLD04_002365"
     CDS             2727..3530
                     /locus_tag="LLD04_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK5649262.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            3551..4465
                     /locus_tag="LLD04_002366"
     CDS             3551..4465
                     /locus_tag="LLD04_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK5649263.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            4548..4922
                     /locus_tag="LLD04_002367"
     CDS             4548..4922
                     /locus_tag="LLD04_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK5649264.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            5061..5306
                     /locus_tag="LLD04_002368"
     CDS             5061..5306
                     /locus_tag="LLD04_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5649265.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            5478..6542
                     /locus_tag="LLD04_002369"
     CDS             5478..6542
                     /locus_tag="LLD04_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5649266.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            6788..7213
                     /locus_tag="LLD04_002370"
     CDS             6788..7213
                     /locus_tag="LLD04_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5649267.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            7206..>7638
                     /gene="nusB"
                     /locus_tag="LLD04_002371"
     CDS             7206..>7638
                     /gene="nusB"
                     /locus_tag="LLD04_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK5649268.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKE"
BASE COUNT         2454 a         1236 c         1715 g         2233 t
ORIGIN      
        1 tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta
       61 ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag
      121 ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat
      181 cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta
      241 tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa
      301 acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag
      361 aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga
      421 atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt
      481 tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag
      541 agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta
      601 gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg
      661 gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac
      721 gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg
      781 atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa
      841 aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa
      901 aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg
      961 tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt
     1021 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
     1081 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
     1141 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
     1201 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
     1261 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
     1321 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
     1381 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
     1441 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
     1501 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
     1561 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
     1621 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
     1681 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
     1741 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
     1801 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
     1861 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
     1921 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
     1981 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
     2041 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
     2101 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
     2161 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
     2221 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
     2281 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
     2341 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
     2401 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
     2461 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
     2521 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
     2581 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
     2641 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
     2701 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
     2761 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
     2821 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
     2881 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
     2941 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
     3001 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
     3061 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
     3121 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
     3181 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
     3241 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
     3301 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
     3361 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
     3421 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
     3481 gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
     3541 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
     3601 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
     3661 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
     3721 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
     3781 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
     3841 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
     3901 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
     3961 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
     4021 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
     4081 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
     4141 tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa
     4201 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
     4261 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
     4321 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
     4381 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
     4441 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
     4501 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
     4561 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
     4621 ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat agacgcaaat
     4681 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
     4741 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
     4801 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
     4861 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
     4921 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
     4981 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
     5041 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
     5101 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
     5161 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
     5221 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
     5281 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
     5341 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
     5401 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
     5461 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
     5521 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
     5581 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
     5641 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
     5701 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
     5761 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
     5821 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
     5881 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
     5941 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
     6001 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
     6061 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
     6121 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
     6181 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
     6241 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
     6301 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
     6361 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
     6421 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
     6481 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
     6541 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
     6601 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
     6661 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
     6721 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
     6781 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
     6841 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
     6901 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
     6961 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
     7021 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
     7081 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
     7141 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
     7201 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
     7261 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
     7321 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
     7381 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
     7441 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
     7501 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
     7561 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
     7621 ccaatgtatt gaaagaat
//
LOCUS       DAFBYI010000118         7623 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFBYI010000118 DAFBYI010000000
VERSION     DAFBYI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.118"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(14..706)
                     /locus_tag="LLD04_002372"
     CDS             complement(14..706)
                     /locus_tag="LLD04_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649269.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LLD04_002373"
     CDS             complement(1143..1712)
                     /locus_tag="LLD04_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5649270.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LLD04_002374"
     CDS             complement(1786..2109)
                     /locus_tag="LLD04_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649271.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LLD04_002375"
     CDS             complement(2263..3075)
                     /locus_tag="LLD04_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649272.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LLD04_002376"
     CDS             3429..4277
                     /locus_tag="LLD04_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK5649273.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LLD04_002377"
     CDS             4422..5363
                     /locus_tag="LLD04_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK5649274.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LLD04_002378"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LLD04_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAFBYI010000119         7596 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFBYI010000119 DAFBYI010000000
VERSION     DAFBYI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.119"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(940..1353)
                     /locus_tag="LLD04_002379"
     CDS             complement(940..1353)
                     /locus_tag="LLD04_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK5649275.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="LLD04_002380"
     CDS             complement(1809..2294)
                     /locus_tag="LLD04_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649276.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="LLD04_002381"
     CDS             complement(2433..2678)
                     /locus_tag="LLD04_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK5649277.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIAGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="LLD04_002382"
     CDS             2871..3671
                     /locus_tag="LLD04_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5649278.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="LLD04_002383"
     CDS             3690..5558
                     /locus_tag="LLD04_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5649279.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="LLD04_002384"
     CDS             5551..6042
                     /locus_tag="LLD04_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK5649280.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="LLD04_002385"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="LLD04_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK5649281.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="LLD04_002386"
     CDS             6602..>7596
                     /locus_tag="LLD04_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK5649282.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccgca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagaag ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DAFBYI010000120         7400 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFBYI010000120 DAFBYI010000000
VERSION     DAFBYI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.120"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            68..151
                     /locus_tag="LLD04_002387"
     CDS             68..151
                     /locus_tag="LLD04_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK5649283.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="LLD04_002388"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="LLD04_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK5649284.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="LLD04_002389"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="LLD04_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1606..2271
                     /locus_tag="LLD04_002390"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="LLD04_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="LLD04_002391"
     CDS             2307..2552
                     /locus_tag="LLD04_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5649285.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="LLD04_002392"
     CDS             2655..3524
                     /locus_tag="LLD04_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK5649286.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="LLD04_002393"
     CDS             3505..4239
                     /locus_tag="LLD04_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5649287.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="LLD04_002394"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="LLD04_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="LLD04_002395"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="LLD04_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBK5649288.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="LLD04_002396"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="LLD04_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK5649289.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6886..>7400
                     /locus_tag="LLD04_002397"
     CDS             6886..>7400
                     /locus_tag="LLD04_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5649290.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga
//
LOCUS       DAFBYI010000121         7344 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFBYI010000121 DAFBYI010000000
VERSION     DAFBYI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.121"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            140..460
                     /locus_tag="LLD04_002398"
     CDS             140..460
                     /locus_tag="LLD04_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649291.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LLD04_002399"
     CDS             912..1112
                     /locus_tag="LLD04_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5649292.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LLD04_002400"
     CDS             1259..4048
                     /locus_tag="LLD04_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5649293.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LLD04_002401"
     CDS             4095..4622
                     /locus_tag="LLD04_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK5649294.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LLD04_002402"
     CDS             4643..5833
                     /locus_tag="LLD04_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5649295.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LLD04_002403"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LLD04_002403"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK5649296.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2439 a         1340 c         1576 g         1989 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtg
//
LOCUS       DAFBYI010000122         7187 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFBYI010000122 DAFBYI010000000
VERSION     DAFBYI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.122"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LLD04_002404"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LLD04_002404"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK5649297.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LLD04_002405"
     CDS             complement(1172..1867)
                     /locus_tag="LLD04_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK5649298.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LLD04_002406"
     CDS             complement(1880..2596)
                     /locus_tag="LLD04_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK5649299.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="LLD04_002407"
     CDS             2926..3534
                     /locus_tag="LLD04_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK5649300.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LLD04_002408"
     CDS             3859..4182
                     /locus_tag="LLD04_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5649301.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LLD04_002409"
     CDS             4364..5734
                     /locus_tag="LLD04_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK5649302.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="LLD04_002410"
     CDS             6081..>7187
                     /locus_tag="LLD04_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5649303.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DAFBYI010000123         6977 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFBYI010000123 DAFBYI010000000
VERSION     DAFBYI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.123"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(362..1480)
                     /locus_tag="LLD04_002411"
     CDS             complement(362..1480)
                     /locus_tag="LLD04_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649304.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="LLD04_002412"
     CDS             1951..3258
                     /locus_tag="LLD04_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK5649305.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="LLD04_002413"
     CDS             3377..4576
                     /locus_tag="LLD04_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5649306.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="LLD04_002414"
     CDS             complement(4603..5871)
                     /locus_tag="LLD04_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK5649307.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="LLD04_002415"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="LLD04_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2115 a         1457 c         1241 g         2164 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaataa
//
LOCUS       DAFBYI010000124         6888 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFBYI010000124 DAFBYI010000000
VERSION     DAFBYI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.124"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(45..1022)
                     /locus_tag="LLD04_002416"
     CDS             complement(45..1022)
                     /locus_tag="LLD04_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK5649308.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="LLD04_002417"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="LLD04_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK5649309.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="LLD04_002418"
     CDS             complement(2595..4019)
                     /locus_tag="LLD04_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5649310.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="LLD04_002419"
     CDS             complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="LLD04_002419"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK5649311.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            complement(5517..6722)
                     /locus_tag="LLD04_002420"
     CDS             complement(5517..6722)
                     /locus_tag="LLD04_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5649312.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            complement(6724..>6888)
                     /locus_tag="LLD04_002421"
     CDS             complement(6724..>6888)
                     /locus_tag="LLD04_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649313.1"
                     /translation="NGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVL
                     MKLMTKEEVG"
BASE COUNT         1946 a         1532 c         1170 g         2240 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 taaagaacta tttttgtttg gctgttctta ttacttaatg caggacaact ttttcacaac
     4261 atcctaatat tttatttatt ttttagattg tgtctccatt aaaaatcgaa tttttcacga
     4321 tgacatagtc aacttttcga atggcttcca gatctcttcc tccagcataa gaaatagatg
     4381 attgaaggtc ttgttgcatt tccactagtg tatctttcaa agatcctttg tgttggatcc
     4441 agattttctt tccttcaaca tttcgttttt cgcctttttg gaattctgaa gcagaaccaa
     4501 agtattcttt atacacgatt ccattttcga cttttgtttc gccaggagat tcctcgtgac
     4561 cggcaaacaa tgaaccaatc atgaccattg tcgcaccaaa acggacagat tttgcaatat
     4621 cgccatgtgt tctgatccca ccgtcagcga tgatcggctt gcgagctgct ttcgcacacc
     4681 atctaagagc tgccagttgc cagcctccag tcccaaagcc tgtcttgatt tttgtaatac
     4741 atacttttcc tggaccaatc ccgacttttg ttgcatctgc gcccgcattt tccaattcac
     4801 ggacggcttc cggtgtacca acattaccgg caatcacaaa agtttcaggc aaatattttt
     4861 taatatgctg aatcatatca ataactgagt tagcatgtcc atgcgcgatg tcgatagtga
     4921 tataatcagg atttagcgat tcttctgcca acttctctat aaaggaatat tcctctttct
     4981 tgacgccaac actgatcgag gtaatcaatc cacgcttttt catttttttg ataaacggaa
     5041 ttcgagctgc ttcatcaaac cgatgcatga tatagaaata accattttcc gctaagaatt
     5101 ctgcgattgt ttcatcaata atcgtctgca tgttggcagg cactactggc attttgaatg
     5161 tatgtttacc taatgtgacc gttgtatcac attcagaacg gctatttacg atacatttat
     5221 taggaattaa ttgaatatct tcgtaatcaa atactttcat tacattatcc tcctgatgtt
     5281 cacatccagc aaaacacgaa cattattaat ttaatcaagg ctttttttag cgccctatgt
     5341 aatttaactc aattaagcaa aaaaatcaag agcaaaaaga gaatttatac gaatggtaaa
     5401 aaaacatata ttatttttag tctatacaaa cgaaacaatg aatgttcgct tttagcgaaa
     5461 aaaagactgt gacaaatgtc ttgtcacagt cttttttctg aataaccggc gttctcctac
     5521 gttgttcgtt ttttcttatt atacaagtct agcacaacgg ctaaaagcaa gatcaatcct
     5581 ttgattgcct gctgccagtc taccccgacg cctaaaatcg acatgccatt gttcaataca
     5641 cccatgacca agccaccgac aatcgcaccc atgattgttc caatgccgcc ggaagtcgaa
     5701 gcaccaccaa aatatactgc cgccattgca tccagttcca tcgatgtacc tgcttgaggt
     5761 gttgccgcat tcaatcgagc agctaaaact aatccagcta aagctgccat gactcccatg
     5821 ttgacgaata cccagaacgt gattcgcttt gttttgatcc ctgatagttc agccgctttt
     5881 cgatttccgc ccgtcgcata aatctgtcga cctgctactg ttttatttgt caaaaaccca
     5941 tatgctgcaa cgatcaaacc aagaatgatc aaaataatcg gatacccttg ataagaggca
     6001 aagatataag ttattcctag tacaacgact gctgtaaata ccgcctttac caagaaagct
     6061 gttggagatt ccacttcaaa taaatgattt ttccgacgat ttcttgttcg ccattgacca
     6121 acaactaaca cgatcgtgat gatcattcct aaaatcaatg tcaagaagtg aaggctgctt
     6181 ccttcgctta agtctggtaa ataacccgta gacatttttt ggaaaacagc tggaaaaggt
     6241 gctagagatt ggcctcctag aacaacttgt gtcagtccac gaaacatcag caatcctgct
     6301 aaagtcacga taaacgccgg tatccctgca taagctaccc aaaaaccttg ccatgcgccg
     6361 atcagcccgc ctactgcaag acatgcgata actgtcatcc acgggttcat ttggcgattg
     6421 accatcatca tgcccgcaat tgcaccaaca aacgccatca ctgaccctac cgacaaatcc
     6481 acatgaccta agagcacaac taataacatc cctgcagcta gtactaatat atggctattt
     6541 tgcagaacaa ggttagttat attcaatgga cgccagaaaa taccatccgt catcaattga
     6601 aacgcaataa gtagtcccgc taaaatgatc accatgctgt agcgaccaaa aagatcagct
     6661 gctctacttt tcaaatttag ttttttctct tttttatccg taagattttt tgcctgttcc
     6721 aacttaaccc acctcttctt tcgtcattaa tttcatcaag acttcctgat ctgcatcttc
     6781 tcgcgagact tctcctgtaa tacgtccttc gttcatcgta tagattcgat cacacatccc
     6841 taatatttca ggaagttcag aagagataag acagacagct ttgccatt
//
LOCUS       DAFBYI010000125         6777 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFBYI010000125 DAFBYI010000000
VERSION     DAFBYI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.125"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            20..397
                     /locus_tag="LLD04_002422"
     CDS             20..397
                     /locus_tag="LLD04_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649314.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1003..1617
                     /locus_tag="LLD04_002423"
     CDS             1003..1617
                     /locus_tag="LLD04_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5649315.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2390..4129
                     /gene="yfcC"
                     /locus_tag="LLD04_002424"
     CDS             2390..4129
                     /gene="yfcC"
                     /locus_tag="LLD04_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK5649316.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4286..5470
                     /gene="iadA"
                     /locus_tag="LLD04_002425"
     CDS             4286..5470
                     /gene="iadA"
                     /locus_tag="LLD04_002425"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK5649317.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5581..5784
                     /locus_tag="LLD04_002426"
     CDS             5581..5784
                     /locus_tag="LLD04_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5649318.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5885..6547)
                     /locus_tag="LLD04_002427"
     CDS             complement(5885..6547)
                     /locus_tag="LLD04_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649319.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6560..>6777)
                     /locus_tag="LLD04_002428"
     CDS             complement(6560..>6777)
                     /locus_tag="LLD04_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5649320.1"
                     /translation="NRQEQGGQRDGQQGTTNNPFAVSDQVSELEINVKPAQLGLLAGV
                     AFGISLFSVFLASIGILRLNPRKILLN"
BASE COUNT         2155 a         1116 c         1420 g         2086 t
ORIGIN      
        1 aaaaaggtgt gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat
       61 actcaagtgg ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag
      121 tctatttatc atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta
      181 tgcagcatcc gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc
      241 ttgcgtattt gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa
      301 aagaaagtct cggataggca gaaaaggtag acatacaact actttttcta gtacagattc
      361 tctcgaacaa agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa
      421 aagataaaat aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc
      481 ttttttcact tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc
      541 attccttttt ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc
      601 ttgcttgcac ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa
      661 aaaaggctcc ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata
      721 caccctgacc gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct
      781 aatctagggt gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta
      841 tttgctatgt caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca
      901 aagaccacat gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag
      961 ctgtttctat tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt
     1021 gactattatc accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct
     1081 ggcacttgga tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc
     1141 acactaagtg gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata
     1201 ttgattgaaa atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt
     1261 attgatcagc tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga
     1321 aacccggcaa tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt
     1381 gttactggat ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta
     1441 tttgaatttg ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact
     1501 ttgccaaatc tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg
     1561 cagcaagttt tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta
     1621 tccacatgtt gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga
     1681 aaaaaagaaa acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt
     1741 ttaagatgga tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag
     1801 ttgtttcaca gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt
     1861 agtggaaaaa gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt
     1921 gatgaaagag tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta
     1981 ttgcaatgaa gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga
     2041 aataaaatac acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt
     2101 tttgcaatga aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc
     2161 tctaatctaa aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat
     2221 aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg
     2281 attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat
     2341 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
     2401 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
     2461 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
     2521 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
     2581 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
     2641 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
     2701 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
     2761 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
     2821 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
     2881 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
     2941 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
     3001 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
     3061 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
     3121 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
     3181 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
     3241 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
     3301 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     3361 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     3421 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     3481 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     3541 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     3601 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     3661 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     3721 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     3781 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     3841 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     3901 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     3961 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     4021 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     4081 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     4141 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     4201 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     4261 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     4321 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     4381 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     4441 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     4501 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     4561 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     4621 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     4681 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     4741 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     4801 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     4861 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     4921 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     4981 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     5041 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     5101 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     5161 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     5221 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     5281 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     5341 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     5401 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     5461 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     5521 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     5581 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     5641 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     5701 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     5761 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     5821 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     5881 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     5941 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     6001 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     6061 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     6121 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     6181 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     6241 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     6301 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     6361 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     6421 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     6481 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     6541 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     6601 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     6661 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     6721 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttac
//
LOCUS       DAFBYI010000126         6610 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFBYI010000126 DAFBYI010000000
VERSION     DAFBYI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.127"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            810..1484
                     /locus_tag="LLD04_002429"
     CDS             810..1484
                     /locus_tag="LLD04_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5649321.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="LLD04_002430"
     CDS             1481..1822
                     /locus_tag="LLD04_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649322.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="LLD04_002431"
     CDS             1852..2211
                     /locus_tag="LLD04_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK5649323.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="LLD04_002432"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="LLD04_002432"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5649324.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="LLD04_002433"
     CDS             5127..6593
                     /locus_tag="LLD04_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5649325.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2188 a         1075 c         1410 g         1937 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta
//
LOCUS       DAFBYI010000127         6604 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFBYI010000127 DAFBYI010000000
VERSION     DAFBYI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.128"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(20..1576)
                     /gene="rny"
                     /locus_tag="LLD04_002434"
     CDS             complement(20..1576)
                     /gene="rny"
                     /locus_tag="LLD04_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK5649326.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1987..3036)
                     /gene="recA"
                     /locus_tag="LLD04_002435"
     CDS             complement(1987..3036)
                     /gene="recA"
                     /locus_tag="LLD04_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK5649327.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3145..4386)
                     /locus_tag="LLD04_002436"
     CDS             complement(3145..4386)
                     /locus_tag="LLD04_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK5649328.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4626..5204)
                     /gene="pgsA"
                     /locus_tag="LLD04_002437"
     CDS             complement(4626..5204)
                     /gene="pgsA"
                     /locus_tag="LLD04_002437"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK5649329.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5413..6336)
                     /locus_tag="LLD04_002438"
     CDS             complement(5413..6336)
                     /locus_tag="LLD04_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649330.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         1746 a         1497 c         1168 g         2193 t
ORIGIN      
        1 tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc cgaataacga
       61 ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga atatcacgta
      121 ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc cgaacttcgc
      181 gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa atattttcca
      241 gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca ggtcttgctg
      301 ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct tccacatctc
      361 catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg gcagctaatt
      421 ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc ccaatatcat
      481 ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct gctaagatac
      541 cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa ctagttctga
      601 atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca ccgacttcaa
      661 acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga gatttctcga
      721 ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt tccaaggtca
      781 tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct tccggtgtat
      841 catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta cgaccttctc
      901 gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa acagtcgttt
      961 ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt ttcgctttgc
     1021 gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt aactcatgat
     1081 tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt gataaaccag
     1141 caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct ttttctcgct
     1201 cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt gagttctcgc
     1261 gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc aaacgattct
     1321 cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca gaacggtatt
     1381 tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct gcttcttttt
     1441 ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct ttctcgtgac
     1501 gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg atagcgagga
     1561 gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta tcatctatac
     1621 agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat atgtgtacaa
     1681 aaaacatgca caactttatt ctaatgtttg taccccgtag tgtcaacttt aaataaacaa
     1741 agaaacggct ttctacttat tcattaaacg atggttttta aaataaatga cactagtttc
     1801 atataggtaa acctttaaat tgagaaactt ttctattaaa ttattctttg tagatgaaca
     1861 gatattttta ttttatgtac ccactcgtat aattaaataa ctcacgattt ctttattcaa
     1921 taaaaaaacg gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca
     1981 taatttttat tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc
     2041 gacttcttca ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc
     2101 aggatgatta gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt
     2161 ataagaatac caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag
     2221 ttctccttct tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg
     2281 aggtgctact ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc
     2341 ttgtttcaat tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc
     2401 gcgtcctcca ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg
     2461 gttgatgaaa atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc
     2521 ttgagacatc aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc
     2581 tgctcgtgga actaaagcag caactgaatc gactactacg atatctacag ccccacttga
     2641 tactaaagca tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag
     2701 ttcatcgata ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc
     2761 aatgaaagcg gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt
     2821 tgttttacct gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc
     2881 gacacctaaa gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc
     2941 gattttttcg cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag
     3001 tgcagcatct aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac
     3061 aaatcagagc aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa
     3121 aaaccgaaca aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa
     3181 tccttgcata accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc
     3241 cgaaaccacg ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc
     3301 taacgtgtcc ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc
     3361 acgtgcttga atggccatct gctccacaca ttctttgctg actgttccat attctgctaa
     3421 tagctccgga tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc
     3481 gcctggaaat acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc
     3541 tgtcaaactt tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac
     3601 ttttgctagc gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat
     3661 tttttcttct aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt
     3721 cagtcgtaat gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt
     3781 ctcaatcaga tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa
     3841 aactttagaa attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg
     3901 ttccacaaac attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt
     3961 atctgtttga taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc
     4021 ttcgataatc tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa
     4081 atatgccaat aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc
     4141 cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat
     4201 gagttcactt cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc
     4261 tacaactgtt tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt
     4321 ggcattcgta ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc
     4381 tttcattact tcccgctccc ttatatagta tacgctgtat ttttatattt ttttatttaa
     4441 aaagagggca aggagctgtg acaaaaatct tgtcacagct ccttgttcca aataaacagt
     4501 gttctcctgc caaaacctcg gtcataagtc acaatataca taaaacatga aaagctgttt
     4561 tttgaaatta tcccttgtga ctcagagtta gacggcaggc tcacaacctc tccactaaac
     4621 gatacttaca ttgatccttt gaacacaccg ctgtttttga tgaagtaatc cacacctgaa
     4681 tagatagtaa agatcagaca agcataaagc atgatttgat ctactggaat accgatcagg
     4741 ctgaatggga tattattgat aaacagcaag ataatagcaa ccatttgtgt agccgtcttg
     4801 acttttcccg gccaagctgc agccatgact tctccatgtt cgacaagtag taaacgcaag
     4861 cctgtgacgg ctaattcacg acagacaatg atcgctacta cccaaccagg ggcctttcct
     4921 tggtcaacca acatgataaa agcagtcata actagcattt tatctgccaa cgggtccgca
     4981 aacttgccaa aattagtgac aaggcctcgc gcacgggcaa tttttccatc taaccagtct
     5041 gtgatactag ctaaagcaaa aataactgca cctactagct gtgtgacagc cagtgtggtg
     5101 tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg
     5161 atcatacaaa ttctaagtac agttaattta tttggtaaat tcacactatc gactcctttc
     5221 ttccctattc tacgtgacta tcagcgaagt ttccaccgat attatttact tttccaaatg
     5281 aatttattca aaggagtatt tttattgaaa ttgatctttt tgaattaacg aagaaggacc
     5341 gcgacacaga tttgtcacga tccttctttt ttatacctac aagtaaacag atgcgtaaac
     5401 taatttatac gatcattctg cgtattgaag agtaaggttc acattttttg gactctgatt
     5461 gttttcccct ggattaactg gtacggattc tccattcgct ttgatcgttg cattgcttgc
     5521 tgcaccaatc gtgatggtcg catttgttgc tgtttccggt aacacggtac tctgtgtttc
     5581 atttgctgcc agtgtccctt gataaacata ggcattgtct actagcacac ctaaccatac
     5641 acgattgaca gcagtaaatt caagggttac aggcttggta gcgtctgtga tatttactgc
     5701 gatggcactg ccagaatctt ctcctgtagc tattgccatt ttcttttctt cacttgattt
     5761 tgttgttgat tctactgtag aagactcttt ggtctgcgtg ctttgctctg aggaagagga
     5821 ctcctcgact gatccttcta ctttaagcga agaagctgtg tctccgatta ttggatcagc
     5881 ttgacgatct tgccaagtca tatagcccac gacaacgaca atcgccaaag caaccaatcc
     5941 taataaaatg acaggaagac ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat
     6001 tgcttttcta gaagtactta ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa
     6061 tttgcttttc ccatcaaaaa catcgactaa atggtctcca tcttctccta cagcttcagc
     6121 gtattgccga ataaatgccc gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt
     6181 gtcgattgct tccaaataac gtttttgaat ttttgtaatc tgctgtaatt catctaaaga
     6241 tattttttta ttcaggcgtg cttgccgtaa tttttctccg atggtttcaa aagccactcg
     6301 ttcatcttct ccagtctgtt tgattctccg aagcatctgc tgttgcaacg aaagacgtta
     6361 tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt tttccgaata aacattgttc
     6421 tcctgccaac tcctcggtca caagtcacaa tatccaataa atataagaag ctgttttttg
     6481 aacttgttcc ttgtgactcg gagttaaacg gcaggctcac gacctctttc ttaaacggtg
     6541 ttcaatcagc tgttcctcgg tattagctca aaaaccggca aaatataagg agatattttt
     6601 ccgt
//
LOCUS       DAFBYI010000128         6453 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFBYI010000128 DAFBYI010000000
VERSION     DAFBYI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.129"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(95..550)
                     /locus_tag="LLD04_002439"
     CDS             complement(95..550)
                     /locus_tag="LLD04_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649331.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="LLD04_002440"
     CDS             complement(704..1912)
                     /locus_tag="LLD04_002440"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK5649332.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="LLD04_002441"
     CDS             complement(2024..2356)
                     /locus_tag="LLD04_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5649333.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="LLD04_002442"
     CDS             2504..3379
                     /locus_tag="LLD04_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5649334.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="LLD04_002443"
     CDS             complement(3461..4042)
                     /locus_tag="LLD04_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK5649335.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="LLD04_002444"
     CDS             complement(4048..5307)
                     /locus_tag="LLD04_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5649336.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="LLD04_002445"
     CDS             complement(5309..6355)
                     /locus_tag="LLD04_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649337.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1799 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aaa
//
LOCUS       DAFBYI010000129         6345 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFBYI010000129 DAFBYI010000000
VERSION     DAFBYI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.130"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1014
                     /locus_tag="LLD04_002446"
     CDS             <1..1014
                     /locus_tag="LLD04_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK5649338.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /locus_tag="LLD04_002447"
     CDS             1025..3229
                     /locus_tag="LLD04_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBK5649339.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3245..3394
                     /locus_tag="LLD04_002448"
     CDS             3245..3394
                     /locus_tag="LLD04_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649340.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3470..4063
                     /locus_tag="LLD04_002449"
     CDS             3470..4063
                     /locus_tag="LLD04_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK5649341.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="LLD04_002450"
     CDS             4076..4975
                     /locus_tag="LLD04_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK5649342.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="LLD04_002451"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="LLD04_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5649343.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="LLD04_002452"
     CDS             5817..6077
                     /locus_tag="LLD04_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649344.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT         2339 a         1095 c         1305 g         1606 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggcttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttg
//
LOCUS       DAFBYI010000130         6159 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFBYI010000130 DAFBYI010000000
VERSION     DAFBYI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.132"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="LLD04_002453"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="LLD04_002453"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5649345.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="LLD04_002454"
     CDS             complement(937..1518)
                     /locus_tag="LLD04_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649346.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="LLD04_002455"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="LLD04_002455"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK5649347.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="LLD04_002456"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="LLD04_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK5649348.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="LLD04_002457"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="LLD04_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK5649349.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="LLD04_002458"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="LLD04_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK5649350.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..>6159
                     /gene="tuf"
                     /locus_tag="LLD04_002459"
     CDS             5973..>6159
                     /gene="tuf"
                     /locus_tag="LLD04_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5649351.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGIT"
BASE COUNT         1857 a         1198 c         1234 g         1870 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaa
//
LOCUS       DAFBYI010000131         6124 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFBYI010000131 DAFBYI010000000
VERSION     DAFBYI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.133"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..189
                     /locus_tag="LLD04_002460"
     CDS             <1..189
                     /locus_tag="LLD04_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5649352.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="LLD04_002461"
     CDS             complement(275..1369)
                     /locus_tag="LLD04_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5649353.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="LLD04_002462"
     CDS             1558..2319
                     /locus_tag="LLD04_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5649354.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="LLD04_002463"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="LLD04_002463"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5649355.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="LLD04_002464"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="LLD04_002464"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK5649356.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="LLD04_002465"
     CDS             complement(3959..4972)
                     /locus_tag="LLD04_002465"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK5649357.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="LLD04_002466"
     CDS             5137..6057
                     /locus_tag="LLD04_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK5649358.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DAFBYI010000132         6077 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFBYI010000132 DAFBYI010000000
VERSION     DAFBYI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.134"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="LLD04_002467"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="LLD04_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK5649359.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="LLD04_002468"
     CDS             complement(2203..4389)
                     /locus_tag="LLD04_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK5649360.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="LLD04_002469"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="LLD04_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK5649361.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="LLD04_002470"
     CDS             complement(4611..5051)
                     /locus_tag="LLD04_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK5649362.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="LLD04_002471"
     CDS             complement(5125..5664)
                     /locus_tag="LLD04_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBK5649363.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1577 a         1368 c         1068 g         2064 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccaacccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgagg
//
LOCUS       DAFBYI010000133         5912 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFBYI010000133 DAFBYI010000000
VERSION     DAFBYI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.135"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            245..2410
                     /gene="acm"
                     /locus_tag="LLD04_002472"
     CDS             245..2410
                     /gene="acm"
                     /locus_tag="LLD04_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK5649364.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2682..3167)
                     /locus_tag="LLD04_002473"
     CDS             complement(2682..3167)
                     /locus_tag="LLD04_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK5649365.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3257..3602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3647..4288
                     /locus_tag="LLD04_002474"
     CDS             3647..4288
                     /locus_tag="LLD04_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5649366.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4307..4942
                     /locus_tag="LLD04_002475"
     CDS             4307..4942
                     /locus_tag="LLD04_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5649367.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4960..5775
                     /locus_tag="LLD04_002476"
     CDS             4960..5775
                     /locus_tag="LLD04_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5649368.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2106 a          928 c         1207 g         1671 t
ORIGIN      
        1 atagattgaa aaaatgaaag caataagtaa aataaaatga atatatgcga aaaaataatt
       61 tacattagga aaaaccttgt aaaaacagtt ttttggcata tttatttttt ataaatattt
      121 gtaaaataac tatcaatttt ttagttaaaa gattggaaat gagtggggga ggaattgaag
      181 aaaggatttt tgagagatga tatagtaggt tttattttta gttaaaatag gggagataga
      241 aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta ctaatataac
      301 atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac
      361 ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc
      421 gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
      481 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
      541 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
      601 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
      661 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
      721 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
      781 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
      841 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
      901 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
      961 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
     1021 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
     1081 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
     1141 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
     1201 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
     1261 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
     1321 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
     1381 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
     1441 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
     1501 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
     1561 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
     1621 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
     1681 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
     1741 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
     1801 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
     1861 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
     1921 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
     1981 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
     2041 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
     2101 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
     2161 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
     2221 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
     2281 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
     2341 tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa
     2401 aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa
     2461 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
     2521 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
     2581 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
     2641 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
     2701 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
     2761 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
     2821 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
     2881 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
     2941 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
     3001 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
     3061 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
     3121 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
     3181 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
     3241 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
     3301 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
     3361 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
     3421 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     3481 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     3541 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     3601 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     3661 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     3721 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     3781 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     3841 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     3901 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     3961 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     4021 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     4081 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     4141 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     4201 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     4261 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     4321 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     4381 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     4441 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     4501 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     4561 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     4621 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     4681 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     4741 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     4801 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     4861 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     4921 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     4981 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     5041 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     5101 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     5161 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     5221 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     5281 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     5341 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     5401 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     5461 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     5521 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     5581 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     5641 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     5701 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     5761 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     5821 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     5881 cctcttggta aacggtgttc aaaagccagt tt
//
LOCUS       DAFBYI010000134         5874 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFBYI010000134 DAFBYI010000000
VERSION     DAFBYI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.137"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            641..1375
                     /locus_tag="LLD04_002477"
     CDS             641..1375
                     /locus_tag="LLD04_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5649369.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="LLD04_002478"
     CDS             1391..2560
                     /locus_tag="LLD04_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5649370.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="LLD04_002479"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="LLD04_002479"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5649371.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="LLD04_002480"
     CDS             3650..4576
                     /locus_tag="LLD04_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBK5649372.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="LLD04_002481"
     CDS             4595..5845
                     /locus_tag="LLD04_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5649373.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DAFBYI010000135         5662 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFBYI010000135 DAFBYI010000000
VERSION     DAFBYI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.138"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(72..542)
                     /locus_tag="LLD04_002482"
     CDS             complement(72..542)
                     /locus_tag="LLD04_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK5649374.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="LLD04_002483"
     CDS             872..2512
                     /locus_tag="LLD04_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK5649375.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="LLD04_002484"
     CDS             2666..3988
                     /locus_tag="LLD04_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK5649376.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="LLD04_002485"
     CDS             complement(4056..4487)
                     /locus_tag="LLD04_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649377.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4552..5388)
                     /locus_tag="LLD04_002486"
     CDS             complement(4552..5388)
                     /locus_tag="LLD04_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK5649378.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1127 c         1037 g         1802 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4681 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4741 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4801 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4861 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     4921 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     4981 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5041 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5101 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5161 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5221 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5281 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5341 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5401 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5461 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5521 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5581 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5641 aagaggtcgt gagcctgccg tt
//
LOCUS       DAFBYI010000136         5561 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFBYI010000136 DAFBYI010000000
VERSION     DAFBYI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.139"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            9..1406
                     /locus_tag="LLD04_002487"
     CDS             9..1406
                     /locus_tag="LLD04_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5649379.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            1487..1927
                     /locus_tag="LLD04_002488"
     CDS             1487..1927
                     /locus_tag="LLD04_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5649380.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            2075..2401
                     /locus_tag="LLD04_002489"
     CDS             2075..2401
                     /locus_tag="LLD04_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5649381.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            2402..3151
                     /locus_tag="LLD04_002490"
     CDS             2402..3151
                     /locus_tag="LLD04_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649382.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(3231..3686)
                     /locus_tag="LLD04_002491"
     CDS             complement(3231..3686)
                     /locus_tag="LLD04_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5649383.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(4113..4388)
                     /locus_tag="LLD04_002492"
     CDS             complement(4113..4388)
                     /locus_tag="LLD04_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649384.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            4682..5497
                     /gene="thiD"
                     /locus_tag="LLD04_002493"
     CDS             4682..5497
                     /gene="thiD"
                     /locus_tag="LLD04_002493"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK5649385.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1877 a          891 c         1123 g         1670 t
ORIGIN      
        1 gtaaaaaaat gaaaacctac acattcccag aaaatttttg gtggggttct gctgctagcg
       61 gaccgcaaac agaaggaaga gtaagtgatg atggtaaagg agaaaatatt tgggatcatt
      121 ggtatgaatt agagcctgaa aaattcttcg atcaagtagg acctgaaaaa acttcgcaag
      181 tttacacaaa atacaaagaa gatgttcagt taatgaaaga aactggccat aacacatttc
      241 gtacatctat ccaatggagt catttgattc cagaagggac aggtgaaatc aatcaaaaag
      301 ctgtttcttt ttatcgagat tattttactg aattgctgga taatggtatt gaaccttttg
      361 tgaaccttta ccatttcgat atgccactac cactgcaaga aaaaggcgga tggttatcaa
      421 gagaaacggt cgacgcttat gtttcttatg cagcagcgtg ctttgaactg tttggagatc
      481 tggtcaaaaa atggtttact cataatgaac caatcgtacc tgtagaagga ggctacttgt
      541 atcaattcca ctatccaaat catatcaata tgaaagaagc ggtccaagta ggctatcatg
      601 agaatctagc aagcgcaaag gtaatcaaag cctaccatga aggcggacat gatggtgaga
      661 tcggaatcat cttgaatctg acaccaagct atccgagaga tgaaacgaac ccagaagatg
      721 taaaagcggc gaagatcgct gacgcattct tcaaccgatc attccttgac cctgcagtga
      781 aaggagagtt tccacaagag ttgatcgaaa ttgtcaaaga attggctata atgccagaga
      841 tccatgaagg agacctagaa ctcatccgtg aaaacacgat tgacctgcta ggaatcaatt
      901 actatcagcc tcgtcgaatc aaagcaaaag aaactccaat caatcacgaa catagcccca
      961 tgccagatga ttattttgat aattacgaca tgcccaataa aaaaatgaat ccatatcgtg
     1021 gatgggaaat ttacgaaaaa ggaatctatg atattttaac gaatacaaga gaaaactatg
     1081 gtaatatcaa atgctatatt tctgaaaatg ggatgggggt cgaaggagaa gaacgatttg
     1141 ttaacagcga aggaattatt gaagatgatt accggatcga gtttgttacg gatcatttga
     1201 aatacgtcca tcaagcaatc caagaaggaa ccaattgtgt gggttaccac atgtggacat
     1261 gtatggacaa ctggtcttgg acaaatgctt ataagaatcg ctatggcttt atcgctgttg
     1321 atttggcaca agacggtaaa cggacgatca aaaaatcagg tcgctggttt aaaacggtct
     1381 ctgaaaaaaa cggatttgat gcctgatatt ttttgataaa acgttcacaa aatgtttgtt
     1441 ctaacactgt aatcgtgcta cactaacagt gaaggaggcg tttacaatgg tagatactta
     1501 tcgtaatatt ttagtagcaa ttgatggttc tgaaaaggca gaaaaagcat ttcttgaggc
     1561 aatcacgatt gccaaacgaa atcaggcaac gttgcacatc ctgtatgtca atgaagtaac
     1621 aggcaattac tttggtgatt ttgcatttgt tacaacaagc cttcaggaag aattggatga
     1681 ggttgcggaa aaccagatga aagaacatcg aaacttggca attgaaaaag gcctgacaga
     1741 tatcgagaca tatgttcttt acggttatcc aaagacactg attgctaatt tccatgaatc
     1801 gaaagaacca atcgatctga ttgtcatggg ggcgacaggt ctgaatgcgg tagaacgtgc
     1861 actagtaggt tccactactt cctatgtagt caaccacgca tcatgtaacg tcatggttgt
     1921 aaaataaata aattaaggga acagaaagca aaaaagcata acgcttcttt tgctttctgt
     1981 tccttttatt tttttgtaaa aaattacttt acctgatgaa gtaatcatgc tattctaatt
     2041 acaacgacag atggagtaat atggaggaaa gaaaatgatt tcaagtgatg gaatacgcgg
     2101 atacaatgat gcgattattt tatcgatcct taacaaaaac gattcttatg gatatgaaat
     2161 ttctaaaacg attcgtgaga ggacaaatga ttcctatgtg atcaaagaga caacacttta
     2221 ctccgttttt gcccgtttag aaaagaatca actgattgtt tcgtatcctg gagaagtgac
     2281 tcatggaaaa aagcggacat attataaaat cactgaaata gggcgtaatc ttttagaaga
     2341 aaaaaagcaa gagtggctga cgacgaaaga cgtagtcgat caatttttgg agggaaatta
     2401 aatggataca atccgtaatt atttagacag tctttttatc ggagtacctc aaagtaccga
     2461 gatagataag ctgaaaactg atttgttggc aaatatggaa gaccattatc atgaattgat
     2521 gggagaagga aaaaatgaac aggaggccat cggtacagta atttcgacat ttggttcaat
     2581 cgatgaatta ttagaagaac tagatgtcga aaaaaaacat caagctgacg agacggaaac
     2641 aaacacagct tctatttatt taagcgaagc agaaaactac tggaaagaat acagagcggc
     2701 aagtctccaa gtggcatcag gtgttctatt cattagctta tcttttgcaa gttttctttt
     2761 cttttgttca gcaggatatg tgttcatggg tatttcttgt ctgattttcg gtatagcatt
     2821 agctgtcggt ttttttattg cttctggtat gaaaatcaca cgtttaaatc actttttgca
     2881 ccatcggaaa atacctgaaa aagtattagc agaagcaaaa gaaaaagaag aagagtatca
     2941 gcgaagtttt ggcttctcac tgattgcagg aattggatta tgtatttttt cacttttccc
     3001 acttttggct tctttaatgt ggtatatgga tggtagtatc ggtgcctcta tattttttgt
     3061 tacggtagga acgggtgtct tcttgatcat ttatggcagt ctagtccgtc attcttatcg
     3121 tcaattcact caaagcgctt attactggtg aatgtattca gatttgtaaa aatattctat
     3181 ttatccggcg tagaaaaaaa gactgtgaca cgcttttgtc acagtctttt ttatatataa
     3241 taatttaaaa attcatcatc taaatccgta gaattttcta gattgcttac ccatgtaaaa
     3301 tcgttataga cagatttcag ttgacttctg cgccattgta cagggataaa aagacgcatg
     3361 ctatcttcat tgaatccgac taataatttc tttccgtcag taatgatggg acgttgtaac
     3421 aaagtaaaat gcgagacgat cagatcgaca agctctgagg tcgtacattg gtcgatttgg
     3481 tctttcaaat ttttataatc aatcgagcgt ttagctaata gatcgtcaaa tccgttttct
     3541 gttaatgaca ataaataaaa tatctctttc cttgttaaag gctgtttagg tgtgaaaatt
     3601 cgttctctgt aaggcatttg atggtctttc agccagtttg ccatcagatg gtaactatgt
     3661 gtgcgagaag aatagatcgt aagcataaaa aaactcctta aatagtccat taatttgtta
     3721 gatctgttga gataaataat aggtaaggag ctgtgcataa gcattgatca aaacaaccca
     3781 aacatcatta gaaataactt tctaaagtct taaacttgcg acgagaaaca actagactaa
     3841 tagaaaaaat gtcttagcca tttggcttcc tgtttgtttt tttgcttatg ctgcagctcc
     3901 tcgggaaagg gttctaacaa tttgaagaag gttgatgatt gatctagttt aaattgtgtg
     3961 aaatttgaaa tctggtgttt ttactagatg aatcacctaa caactatgat tataacttat
     4021 agaacttctg ttttgcattc ggttttgtta tagttgttat aaagttttat tttattacag
     4081 aacattttta attaataaag aacagttaga agttaaaatt gctgattcac atagagttta
     4141 tgacttcttg gaaattcttt ttcaatcagt tttttcatct gtttatgaaa ttcttttttt
     4201 tcagtaaaca taaaggtttg ggtatcgtaa ccttcttcat gtaggaaata gattattcct
     4261 ttttctgtgt tttgcgtata agcttttata ctcctgtctt ttttgaaaga acagatcgta
     4321 tagccatcta tttgctctgg tagtcgtttg attttcttca aaatgacttt tttatcctca
     4381 agtttcattt ttttcacctg actttatttt tttcatatgg aaatcatagc gcctttggtt
     4441 atatgaaaac aagaaggata ctgaaatata tataaatcaa atagtggtta tttattatta
     4501 gaatagcgaa tacaaataaa cgaccactat tttctgattt attgcaaatg ttttttcaaa
     4561 taaatttgat taccaagaaa aggtagtggt atatttactg agggatactt tgaaaaacat
     4621 atgatagaaa cagtaacagt attatgaaaa atctttttat aaaaatggag gaatcgattt
     4681 gatggaagat cagtatgtac aagcaatgac gatcgcagga tcagatagcg atggaagcgc
     4741 agggatgcaa gcagatatgc atacattctt tacaagaaat gtttacggtg tttctgtcat
     4801 gaccgcatgt gttgcaggta attcttacgg aatcggagca agtgtgacat tgccaacaga
     4861 ttttatcgac aaagaatttg aactgattgc gaaggacttt caaatacgtg cagctaaaac
     4921 aggtatgcta gcagattcaa agttgattga gacagttgta aaaaattacc aaaaatatga
     4981 ttttggacca ttagtcgtgg atcccgtaat cgtaacgaaa catggaaatc ttttactaga
     5041 agaatctgct ttgcaaagct tgaaagaaaa gcttgtccct ttggccgaag tactgacacc
     5101 taatttttat gaagcagaaa agctgactgg acggacatta gcagctgaag aagattttgt
     5161 gcaagcagca aaagaactgc aagatatggg tgcaaaaaac gttatgatca aaggacatca
     5221 tgctaaaaat gcgcagcaag aaacagtcaa tgactacgta ttgttcgaat caggaaaatc
     5281 attttggtta aatgcaccat tttatcctac cgaccggatc aacggaactg gcgattcgct
     5341 atctgcatgt attgcagctg agttagcgaa aggaaaaaca gttgaacaag cgatccgatt
     5401 ggcaaaagac tatgtaaacc gtgcaattcg tgaagaaata aatgtagggc acaaatatgg
     5461 ccccatcaat cattgggcag ctggtgaatt agagtaaaag aaagaagttg tgaaaaagtt
     5521 gccctgcatc aagtaataag aacagccaag taaaaacagc t
//
LOCUS       DAFBYI010000137         5558 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFBYI010000137 DAFBYI010000000
VERSION     DAFBYI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.140"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            132..2192
                     /locus_tag="LLD04_002494"
     CDS             132..2192
                     /locus_tag="LLD04_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK5649386.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2275..2844
                     /locus_tag="LLD04_002495"
     CDS             2275..2844
                     /locus_tag="LLD04_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649387.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3019..3825)
                     /locus_tag="LLD04_002496"
     CDS             complement(3019..3825)
                     /locus_tag="LLD04_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649388.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3963..5477
                     /locus_tag="LLD04_002497"
     CDS             3963..5477
                     /locus_tag="LLD04_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK5649389.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1979 a          964 c         1121 g         1494 t
ORIGIN      
        1 tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt ttgattttat
       61 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
      121 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      181 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      241 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      301 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      361 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      421 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      481 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      541 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      601 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      661 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      721 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      781 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      841 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      901 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      961 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
     1021 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1081 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1141 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1201 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1261 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1321 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1381 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1441 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1501 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1561 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1621 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1681 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1741 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1801 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1861 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1921 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1981 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     2041 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2101 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2161 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2221 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2281 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2341 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2401 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2461 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2521 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2581 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2641 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2701 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2761 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2821 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2881 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2941 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     3001 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3061 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3121 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3181 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3241 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3301 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3361 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3421 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3481 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3541 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3601 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3661 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3721 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3781 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3841 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3901 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3961 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     4021 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4081 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4141 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4201 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4261 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4321 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4381 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4441 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4501 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4561 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4621 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4681 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4741 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4801 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4861 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4921 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4981 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     5041 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5101 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5161 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5221 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5281 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5341 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5401 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5461 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5521 gctaataccg agggtcagct gattgaacac tgtttagt
//
LOCUS       DAFBYI010000138         5552 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFBYI010000138 DAFBYI010000000
VERSION     DAFBYI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.141"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(155..850)
                     /locus_tag="LLD04_002498"
     CDS             complement(155..850)
                     /locus_tag="LLD04_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5649390.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            1004..1282
                     /locus_tag="LLD04_002499"
     CDS             1004..1282
                     /locus_tag="LLD04_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649391.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1301..2509
                     /locus_tag="LLD04_002500"
     CDS             1301..2509
                     /locus_tag="LLD04_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5649392.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2657..3022)
                     /locus_tag="LLD04_002501"
     CDS             complement(2657..3022)
                     /locus_tag="LLD04_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK5649393.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3169..3942)
                     /locus_tag="LLD04_002502"
     CDS             complement(3169..3942)
                     /locus_tag="LLD04_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5649394.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4116..4976)
                     /locus_tag="LLD04_002503"
     CDS             complement(4116..4976)
                     /locus_tag="LLD04_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK5649395.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4976..>5552)
                     /locus_tag="LLD04_002504"
     CDS             complement(4976..>5552)
                     /locus_tag="LLD04_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBK5649396.1"
                     /translation="SLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNA
                     PIAACMAAGMTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDP
                     IRVIPSLMTGSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTT
                     AFMLGLLKKTVKEKKQDKWTKNENLTNEVEA"
BASE COUNT         1625 a         1126 c          945 g         1856 t
ORIGIN      
        1 agttaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
       61 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca
      121 ggtcaaaacg ctgatttcac aacctctttg ttcatcaaaa gataatattg tcttctaaca
      181 agtccatatt caaaataacg atatgatggt ctttaacggt gataatacca tcttttttca
      241 gttgttttaa aattctgttt acactgctag cagttgagat gccacaaaat ttggcgattt
      301 cttcattcgt gatcgaaaaa tcgatcagca tcccttcttc tgtccgcaca ccgaataaat
      361 catataattc gtaaagttgc gtacaaacag ctcccagctt tccgtttaat agcatttgct
      421 gcattttttt catcgaataa agcagacgat agcggtaata ctcttttaca tagtgttgta
      481 gctttgggtc ttggttgata ttttgccaaa agcgcacacg attgattcga taaaactctg
      541 catattctga ttcaactcga atattatacg gcgaatcgat gaattgtgaa tattcgtctt
      601 ttaaaagaga gacgatttca agatctttga tatagcgaag gttaaattct ctcccatcat
      661 ttgaaataac acttgctttt accgttccct ctttcaagat atacgtatgg gaatcttcca
      721 tcccttcgaa tgtcaaatat ttatgatatc ctttgcgtat aatcggaaaa tcatttttat
      781 ccaaatattc ctttaagact ctactatcca atgatgctcc cccatccaaa ctttcatcta
      841 aaaagatcat acagtacttt aaaacttttt aaagtccaaa ggtattttac taatgatatt
      901 aacatttgtt aagtaccttt ttccatcact tatgcgattt ttttcctttt ttttattcgt
      961 tatactgcag aagtaaaatt ttttaagaat agagggaaaa aacatgaatt actggatgaa
     1021 cacaatcatt aatcgattag aaacagcata tcaaacaaga tttgatatga aagcttcttt
     1081 agtcttttta aatgatgcgt atcaaaactc gatcgaattg atcaaagcag tggatgagaa
     1141 tccaacaaac gaatgcgaag aatttttaaa tctgtttatg tctacaagag atttatttat
     1201 tcgccaactt gttgatcgct atccatccaa ttatcacgat gtagaagttc aaatacaaaa
     1261 attaaaagca tattcagctt aaggggaatg tataacatac atggaaaaga aattatttaa
     1321 taaatatttt attggaatca cattgatcaa ttttattgtc tacctgattt attatctgtt
     1381 gatggtcatc atggcagtca ttgcgcaaga caccttacac gccagcttga gccaagcagg
     1441 ttttgcttct gggatctata tcatcggaac attgctggca cgcttattca tggggaaaca
     1501 gttagaacta tttggtcgga agtttacctt aagaggcggc gcgatcttct atctcctttc
     1561 aacattagca tatctttata cgccaactat cggggcattg tatttagttc gattcttgaa
     1621 tggattcggc tacggtacag tttctacagc tacaaatgct atcgttactg catatatccc
     1681 tgcatctaaa aaaggggaag gcatcaatta ttatggatta agtactagtt tagctgccgc
     1741 tgttgggcca tttttaggaa tgattttgct gaatatgact gattttcgtt tcattatctg
     1801 gttttcaatt gtactggtct tctttgttac gattgcctgc cttgtctttc ctgtaaaaaa
     1861 tattccatta tctgaagaac aaaaaatatc tcttcgctca tggaatatca atagttttat
     1921 tgaaaagaaa gcacttttca ttacaggaat tgcctttttg atgggtcttt cttattcaag
     1981 tgttttatca tttttatctt cttatacaaa agtaatccac ttagttgctg ctgggtcatt
     2041 tttcttcgtc gtatatgctt tggtcatcac atttacacgt ccattgactg gaaggatttt
     2101 cgatgtcaaa ggagaacaat acgtgatgta tcctagctat ttattcttaa cagcaggatt
     2161 atttttactg agtgtaacga cgaatagtgt gacattgctt atctctggag cattagtcgg
     2221 cctaggttat ggtacgttta tgtcaaatgg acaggctgtt tgtttaaaaa tcgtccgcga
     2281 acatcgaatc agtattgctt tatctaccta ctttattggt ttagacctag gcttaggcgt
     2341 tggtccttat ttgatgggga tgctaaaatc tcttacttca tttagaggat tgtatgtgat
     2401 tgccggagtc attccattga tatgtacagc actctatctt gttaatggcc gaaaacatag
     2461 tcatgaagca gagggcgaat tgattgaatc attgaaaaat gaaatttgat cttgatgaat
     2521 agataaaaaa aaccgttcag atctgaaaat aatctgagcg gttttttgag taaaaatagt
     2581 ttatgattat tcttaattaa acatctattt atttttaaag ctttcattta tcgataagaa
     2641 tagtttaact gttgatttat tccattttcc gttcatctgg tacgatgcct tgacttagca
     2701 accacttgtg gaagaaccat tctgccacac cgatcaatag agcaggaatc aatactttca
     2761 tccaaatatc gactgtatca ttcataaaat atagatagag ccacaagata actgcagctg
     2821 aaccagcatc tgcgatcgtc gctgcgatgt tccctacttt acgtaagatc aacagatccc
     2881 ctagcacata gatcatgact acaataatta ccccaacaat cgttgaatcc ataaagctaa
     2941 caccgtataa acctgtcagc acgacccaca gaatcgccca gatcatgatt gccttcacta
     3001 ccaacgcctt cacatgtttc attttctgca ctcctttctc ctggataaaa aagtaagatt
     3061 atctaacaag ataacctttt cattattata ccatcggctg agagaagcga gcaaagataa
     3121 ggtcctatct aaaaatgagg aagccactta gaacattcac gtaataattt atccgtacag
     3181 acattcgata tggtgttcct ttactttcgc atactgttct tttgtcagtt ctttacttgt
     3241 caccagataa tcgatttcat gaaaagcaat ggcttgatgc atgaatttat gtgcaatttt
     3301 ttgctgtgta actaaaacgg caagccggtc tgattgtttt ctaaaagctt ttttgaccat
     3361 tgcctctcct tcatgagttt ctgttactcc ttggctgaaa tctagcccgc gacaactcat
     3421 tatcgctaaa tccgcataat atgacaatgc atcgttatag gcttttgaac cgttgattgt
     3481 tgctcgtttc ggaacaattg cccctcccaa tacagatact tttgccgttg tatattcact
     3541 caacatagca gctgtgacga tgccattcgt aatgatctgc aaaccatcaa atcttgctag
     3601 gtttctgact aagtgtaaag aggttgtact ggaatccaag aataagacca tatcatctcg
     3661 aatcaaagaa gaagctgtat ctgcaatctt acgtttttct gtttcttctt ctaatttggt
     3721 gatcttcatc aggtaaggca cgtcaatttt tcgattgtca ataagcatgg cgccaccgcg
     3781 aactctacgg atcaatccac ttttttccag aaaaacaagg tcacgtttaa tcgtggcttc
     3841 gctataattt actaactcga tcaaatcggt gactcttgca aattggttgt tttttagata
     3901 attcacaatc tcctgctgtc tattctccac ttcattcaac ataaaatcac cccatttact
     3961 ttatatagta acaggaaaaa agaggttgtg acagaagtga ttggcttcga gaaataagaa
     4021 gaaatttttc gaaaaaacta cttctgaaca tcgtgtgtgg ttaagagggt atgacaagac
     4081 tattgtcata ccctcttgac cacacatttt tatttttagt ataaactagc cttgcctact
     4141 gtgttcaaaa tttccatttt atgtttgacc acttcttgta cctttttatt agctgctgga
     4201 taaattgcat tcggctcgta catcgatgga ttttcactca atatttctct caaagcattg
     4261 aaaaagacgc ttttaagatc tgagctcaaa ttcactttgg caactccgta ttttactgct
     4321 tcgctgactt ctgcgtccgg attacctgaa ccgccatgta aaacaaatgg aagatcgatt
     4381 tgttggttca attctttcag taagtcgata tttaattttg gagttctgtt tttcggatac
     4441 aagccatgcg ctgttccaat tgctacagca agtgtatcga ttcctgtttc tttcacaaaa
     4501 atcttcgctt gatcaggatc tgtataaatg attgtttcag cacctccttc agcagaacca
     4561 ttattcccaa tcgttccaag ctcagcctcc actgagacat ttaccgcatg agctaacgca
     4621 accacttttt tcgtaatctg tacattgtcg tcgaatgatt tcaaagacgc atcgatcatc
     4681 acggaagaat agccgtttcg aatcgcccgt aagacatctt ctatcgtacc gccatgatcc
     4741 aagtggataa cgatcggcag tttacttgta taagcatagg ctcttactgc cgcaacaaac
     4801 tcatcactta aataggcgat ttcatcagga tgaatttcta agattactgg tgcttctaat
     4861 ctctctgctt cttcgatgat tgctttcatc atatcatagc tgcaaatatt gaaggctggt
     4921 actgcaaatt gattctcttt agccactttc aataactctt tcattgtata taacattacg
     4981 cttccacctc atttgttaga ttttcatttt tcgtccactt gtcttgtttt ttctctttca
     5041 ctgttttttt caatagtcca agcataaagg ctgtagtgac tgtacctgat gcaattgcca
     5101 agacatacat aggtaaattg ttcataacat ttggaattag catcacccaa atccctccat
     5161 gaggtgccat tgaagtaact cctactgcca tggaaatccc tgcagcgact gctgaacctg
     5221 tcatcaaact aggtatcacg cggatcgggt cagataccgc aaacggtata gcaccttctg
     5281 taatatacga agcgcctaaa atccagcatg attgacctga agtccgttct tctttggtaa
     5341 atttatgttt gaataataaa gtggctaaag caatacctag gggaggtgtc attcctgcag
     5401 ccatacaagc agcaatcggt gcatttactc cagctgacat caaaccgatc gagaaagtcg
     5461 aagtcgattt attgatcggc ccacccatat cagctgccat catgatcccg ataagtaatc
     5521 cgaacaatag cccggatgta tttcccaagc ta
//
LOCUS       DAFBYI010000139         5300 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFBYI010000139 DAFBYI010000000
VERSION     DAFBYI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.142"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..429)
                     /locus_tag="LLD04_002505"
     CDS             complement(<1..429)
                     /locus_tag="LLD04_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649397.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGF"
     gene            complement(407..901)
                     /locus_tag="LLD04_002506"
     CDS             complement(407..901)
                     /locus_tag="LLD04_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5649398.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(921..2141)
                     /locus_tag="LLD04_002507"
     CDS             complement(921..2141)
                     /locus_tag="LLD04_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649399.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2288..4123
                     /gene="lepA"
                     /locus_tag="LLD04_002508"
     CDS             2288..4123
                     /gene="lepA"
                     /locus_tag="LLD04_002508"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK5649400.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4217..>5300)
                     /locus_tag="LLD04_002509"
     CDS             complement(4217..>5300)
                     /locus_tag="LLD04_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5649401.1"
                     /translation="INDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1618 a         1046 c         1022 g         1614 t
ORIGIN      
        1 aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa
       61 gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat
      121 tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat
      181 gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt
      241 caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt
      301 gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac
      361 gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag
      421 tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt
      481 ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag
      541 ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag
      601 tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc
      661 cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt
      721 ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt
      781 cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta
      841 actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca
      901 tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg
      961 atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa
     1021 taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg
     1081 gattttccta taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg
     1141 aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct
     1201 gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa
     1261 tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt
     1321 ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat
     1381 aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca
     1441 gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct
     1501 gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat
     1561 aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca
     1621 actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg
     1681 attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata
     1741 ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg
     1801 ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc
     1861 acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt
     1921 tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt
     1981 tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca
     2041 gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc
     2101 ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt
     2161 agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa
     2221 actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg
     2281 agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat
     2341 catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca
     2401 cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga
     2461 acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga
     2521 aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc
     2581 tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga
     2641 agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt
     2701 catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga
     2761 cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat
     2821 tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc
     2881 accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa
     2941 tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg
     3001 caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt
     3061 tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg
     3121 agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg
     3181 aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga
     3241 tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga
     3301 aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga
     3361 tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc
     3421 cgttatttac catgttaata aaacggatgg tacgactgct acagtagata acccggcaga
     3481 ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat
     3541 ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt
     3601 cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc
     3661 tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt
     3721 ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa
     3781 tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg
     3841 aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat
     3901 ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa
     3961 cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca
     4021 aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt
     4081 catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa
     4141 acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt
     4201 ttttcggtgg caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat
     4261 ttctcatctt atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc
     4321 ccaatcgttc agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag
     4381 tatgtctgag agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat
     4441 tccatttcac tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg
     4501 aatcaatgta gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt
     4561 tcagaattgc tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg
     4621 gcgtaccata tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc
     4681 catttgaaaa atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg
     4741 tataaaatcc aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca
     4801 tatgatcaaa gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt
     4861 catattttgg tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa
     4921 ccgccatatt cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt
     4981 aacccttttt aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa
     5041 gttttttctt tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat
     5101 aggtattgta ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt
     5161 ttactcgagt atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt
     5221 ttcgtaaagc agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc
     5281 ttgagccatc atcattgata
//
LOCUS       DAFBYI010000140         5278 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFBYI010000140 DAFBYI010000000
VERSION     DAFBYI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.143"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1537
                     /gene="ftsH"
                     /locus_tag="LLD04_002510"
     CDS             <1..1537
                     /gene="ftsH"
                     /locus_tag="LLD04_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK5649402.1"
                     /translation="AEEEKQELVEVVEFLKDPRRFVELGARIPAGVLLEGPPGTGKTL
                     LAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETAKKNAPAIIFIDEIDAVGR
                     QRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATNRSDVLDPALLRPGRFDRQ
                     ILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGFAGADLENVLNEAALVAAR
                     RNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYHEAGHTIVGLVLSRARVVH
                     KVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGGRTAEEIIFNVQSTGASND
                     FEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQTKAYSEQVAFEIDQEVRK
                     ILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKSLFEHGVMPEGTESNDYPS
                     EKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEENKAEHDSDSQQSEDNHSDD
                     QYK"
     gene            1754..2641
                     /gene="hslO"
                     /locus_tag="LLD04_002511"
     CDS             1754..2641
                     /gene="hslO"
                     /locus_tag="LLD04_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK5649403.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2642..3646
                     /gene="dusB"
                     /locus_tag="LLD04_002512"
     CDS             2642..3646
                     /gene="dusB"
                     /locus_tag="LLD04_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK5649404.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3757..5253
                     /gene="lysS"
                     /locus_tag="LLD04_002513"
     CDS             3757..5253
                     /gene="lysS"
                     /locus_tag="LLD04_002513"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK5649405.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1821 a          935 c         1224 g         1298 t
ORIGIN      
        1 tgcggaagaa gaaaaacaag aactagttga agttgttgaa tttctgaaag atcctcgccg
       61 atttgttgaa ttaggtgccc gtatcccggc aggtgtactt ttagaaggac ctccaggaac
      121 aggtaaaacc ttattagcaa aagctgttgc cggagaagct ggagtaccgt tttactcgat
      181 ctcgggttct gatttcgtag aaatgtttgt cggtgttgga gcaagtcgtg ttcgtgattt
      241 gtttgaaaca gcgaagaaaa acgcaccagc tatcattttc attgatgaaa ttgatgcggt
      301 aggtcgtcaa cgtggtgccg gtatgggcgg cggacatgat gaacgcgaac aaacattgaa
      361 ccaattgctt gtagaaatgg atggcttcga cggaaatgaa ggagtaatcg tcattgctgc
      421 aaccaaccgt tctgatgtac tagacccagc gttactacgt ccaggccgtt ttgaccgcca
      481 gattttagtt ggacgtccag atgtaaaagg tcgtgaagca atcttgcgag tccatgcacg
      541 gaataaaccg atggcagatg acgttgactt gaaagttgtg gctcaacaga caccaggttt
      601 tgccggagca gatttagaaa atgtcttgaa tgaagcagcg ttagtagctg ctagaagaaa
      661 taaaaagaaa atcgatgctt ctgatatcga tgaagcggaa gatcgcgtga ttgcaggtcc
      721 agctaaaaaa gatcgcgtaa tcagcaaaaa agaacgcgaa atggttgctt atcacgaagc
      781 tggtcatacg attgtcggat tagtattgag tcgcgctcgg gtcgtccaca aagtaacgat
      841 catccctcgc ggacgcgccg gcggatatat gatcgcttta ccaaaagaag atcagttctt
      901 gatgacgaaa gaagatatgt ttgaacaaat cgtaggctta cttggtggac gtacagctga
      961 agaaatcatc ttcaatgtcc aatcaactgg tgcttccaat gactttgagc aagcgacagc
     1021 tctagcacga agcatggtaa cagaatatgg aatgagtgat cgtttaggtc ctgttcaata
     1081 tgaaggaaac catcaagtgt ttgttggccg cgactatggt caaacaaaag cttactcgga
     1141 acaagtcgct ttcgagattg atcaagaagt acgaaaaatc ttgatggaag ctcatgataa
     1201 agctcgagaa atcatcgaag ctcatcgtgc acagcataaa ctgattgctg aaaaactttt
     1261 ggaattcgaa acattggatg ccaaagcaat caagtcattg tttgaacacg gtgtgatgcc
     1321 tgaaggtact gagtcaaatg attatccaag cgaaaaagaa gcgaaaactt ttgaagaagc
     1381 aaaacgtgct ttggaagaaa aagacgctga aaaacaagcg gaagaaaaac gtgattttga
     1441 agaagcaaaa catgaactga atgaggaaaa taaagcagaa catgattcag attctcaaca
     1501 atcagaagat aatcattcag acgatcaata caaataaaaa aatataggtc aattgatctg
     1561 atatgactcc cgaaaaccgg aacaaaaacc ggttttcggg agatttcttt atttaagttg
     1621 taaatagggt gatgtcctgt tttctagatg attctgaatt atttttaaaa gaagcattcc
     1681 aaagttgaaa aaagcagtag ttgctagtat gattcactat gagtttaaaa acggaaaaag
     1741 gagtacataa caaatgaaag attatttggt taaggcactt gctcacgacg gattcgtccg
     1801 agcatacgcc gttcaagcaa caaataccgt agcggaggca caaagacgtc atgatacttg
     1861 gcacacctcc tcagctgctt tgggacggac attggtcggt agtctgctac ttggtgcgac
     1921 gctcaaagga gaagacaaac taactgttcg aatccaaggc gatggtccag caacaggaat
     1981 tgtagtagat gcaaatggaa agggagatgt gaaaggttac atcaaaaatc cgcatctcag
     2041 tcttcctcta aatagtgaag gtaaaatcga tgtgcgggga gcagttggaa cacaaggaat
     2101 gtttactgtc acaaaagact taggattaaa agaacctttt tctgggcaaa cgccgattgt
     2161 ttctggagaa atcgctgaag attttactta ctatcttgct atctcagaac aaatcccttc
     2221 agctgttgga ctgagtgtat tggtcaatac tgacgacagc atcaagacgg cgggaggatt
     2281 catgatccaa atcatgccag gtgccgatga agaaacaatc agcaagatcg aagaacaatt
     2341 aaaaacgaca ccaagaatct ctaccctctt agatgaagga cagacaccag aagaaatatt
     2401 gaaaggattg cttggtacag aagaattgaa cattttggaa aaaatgcctg ttcaattcaa
     2461 atgtgactgt tcaaaagaaa aatttgcaga agcaattatc gcgttaggtg cagaagaaat
     2521 ccaagcaatg atagacgaag atcacggtgc agaagcggtt tgttctttct gtaataacaa
     2581 atacaagtat agcgaagaag aattagatgg attgaagaaa gaagccttag gagggattta
     2641 agtggaacac acatggaaaa ttggcaatgt ggaaatccct aatcgtgtcg ttgttgcacc
     2701 gatggctgga ataacaaatt cagcatttcg agtcactgtc aaagaatttg gtgcgggctt
     2761 agtcgtatgt gaaatgatca gtgaccaagg tattcatttc agaaacaaaa aaactttaga
     2821 gatgctccac attgaagaaa cagaacatcc attaagttta cagatatttg gcggaaataa
     2881 agatagtcta gttgaagctg ctcaatttgt cgaagaaaac acagctgccg atataataga
     2941 tatcaatatg ggctgtccag taaataaagt aatcaaagca gaagcaggag caaaatggct
     3001 tttagatcct gagaaagtat acgaaatggt ccaagctgtt tcttcagcag taaatatccc
     3061 agtaacaatc aagatgcgta ttggttggga tgaagagcac gtctttgctg tagaaaatgc
     3121 attagcagca caatcagctg gagcaagtgc catcgctatg catggacgta cacgggttca
     3181 gatgtatgaa ggaaaagcgg actgggaagt attgaaagaa gtgaaaaaac atttgacgat
     3241 cccatttatg gggaacgggg atgtcaaaac gccagaagat gcaaaatgga tgcttgacta
     3301 tgtcggtgca gacggtgtga tgattggtcg tgcagcgctg ggcaatccat ggatgattca
     3361 ccgcacacag cattatctcg aaacaggaga actgattcct gaaccaacac cagccgaaaa
     3421 aattgctaca gctaaacttc atttacaacg cttagcagat ctcaaaggag aaaaagtagc
     3481 ttgtagagaa ttcagaacac atgcggctta ttatttaaaa gggattcctc gagcagcgaa
     3541 agtgaaagta gcaatcaatc agacagaaaa acagcaagaa atcaatgacc tgctggatca
     3601 atttgtagaa aaagcggaaa aagtactatt gaaacaaacg ttgtgatttc taagtttttc
     3661 gtgaattttc aagccgaaat tccaagttcg gcttgctttt tttgtgtttc attggcatta
     3721 taggaaagag attaacatga gaatggagga acttttgtga cacaggaaca acaacatcaa
     3781 gtagaagatt taaatgatca aatgcttgta cgtcgtcaaa aaatggaaca attgcgtgaa
     3841 gatggaatcg atccatttgg aaaacgattt gaacgcacgc acaactcaga agaattgcat
     3901 gaactatttg atccaagaac aaaagaagaa ctagcagaaa tgggattgac cgctagtgta
     3961 gctggtcgta tgatgactcg ccgaggaaaa gggaaaactg gatttgcgca tctacaagat
     4021 cgtgaaggcc aaattcaaat ttatgtaaga aaagaccaag taggtgatga ggcctatgaa
     4081 gtattcaaac atgcagacct tggagatttc tttggtgtaa ctggacaaat catgaaaaca
     4141 gacacaggcg aagtttctat caaagcttca gaaatcacga ttttgtcaaa agcacttcgt
     4201 ccactgccag ataaatacca tggcttaacg aatattgaac aacgctacag acaacgctac
     4261 ttggatttaa tcagtaatcg cgaaagcttt gaccgattta tgaaacgaag ccaaattatc
     4321 agcgaaatcc gtcgctattt agatggcaat ggctatattg aagtagaaac accagtattg
     4381 cacaatgagg caggtggagc tgccgcacgc ccatttatca cgcatcataa tgcactggac
     4441 attgatttat atttgcgtat tgcgctagaa cttcatttaa aacgactaat tgttggcgga
     4501 atggaaaaag tttatgaaat cggccgcgtt ttccgtaatg aaggaattga tactacacat
     4561 aatccagaat ttacgatgtt agaagcatac acagcatata ctgatttcaa tgatgtaatg
     4621 aatctgacag aaggtattat ccgtaacgca gctgaaaaag tactaggaac agcaaaaatc
     4681 acatatgatg gacaagctgt cgacttagaa agtgacttta aacgcatcca tatggtagat
     4741 gcaatcaaag aacaaactgg tgtagacttc tggcaagaaa tgacattgga agaagcatta
     4801 gctttagccg aaaaacacaa tgtagaagta accgaagcta tgggtgtcgg acatgttatc
     4861 aatgaattct ttgaaacatt tgttgaagaa acattgacac aaccgacgtt tgtatacgga
     4921 catccagtcg aagtttcgcc tttagcgaag aaaaatccag aagatcctcg attcacagat
     4981 cgattcgaac tattcatagt ggggcgcgaa tttgctaatg cctttacaga actgaacgat
     5041 ccaattgatc aaagagaaag attcgaagcg caagaaaaag aacgtgaact aggaaatgac
     5101 gaagctcacg gtgtagacga agacttcttg gaagccttag aatacggtat gccgccaact
     5161 ggtggtctag gcatcgggat cgacagatta gtaatgttgc taactgatgc acaatcaatt
     5221 cgggatgttt tgctattccc aacaatgaga taatcgaata aaagctgtct aaggaatt
//
LOCUS       DAFBYI010000141         5144 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFBYI010000141 DAFBYI010000000
VERSION     DAFBYI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.144"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            100..2113
                     /locus_tag="LLD04_002514"
                     /pseudo
     CDS             100..2113
                     /locus_tag="LLD04_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            2239..2568
                     /locus_tag="LLD04_002515"
     CDS             2239..2568
                     /locus_tag="LLD04_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK5649406.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2608..3612
                     /locus_tag="LLD04_002516"
     CDS             2608..3612
                     /locus_tag="LLD04_002516"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5649407.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3609..5038
                     /locus_tag="LLD04_002517"
                     /pseudo
     CDS             3609..5038
                     /locus_tag="LLD04_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1837 a          977 c         1014 g         1316 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gaatcttact tacggaagtg ctgcacaaga
      961 cctgacgact aatacgccta ctatcaacgg atataccttt acagctgttt cagcgacaga
     1021 aacaaagaac caatcagcaa tgagtgtttt agctagttta gatacaaatg gaaatgtaat
     1081 cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc ctatattata agacaaatgc
     1141 aacagtatca atcaatgcaa atggcagtaa gtactatgat ggtctttctg ttgtaccgat
     1201 cgttaaatat gcctttaatg ataaaacaga atcaacatcc cttttaaaca atgatctcag
     1261 tattgatcaa atcacatgga atccaactga tttcaaagcg atgaatgaaa agggagttga
     1321 agtaactgcg cctactgaaa ttggaaccta tacgtcttgg caattgacaa aaagcggact
     1381 agctaaacta gctgccagaa caaactattt atttactatc gtgcagacat ctgatgtcta
     1441 tacgattaag caaatcagcg gtgcggtaga gctcagtggc agtaagacat atgatggaaa
     1501 agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt gtcactagtt cgcttactcc
     1561 agtacaagtg gcacttagca gtatggatta cacagcagat gcacaatcag caaaaaatat
     1621 ggttaatgct ggcagctata caattaaact aacgaatagc ggaatagaca aagtcaaagc
     1681 agcaaatccc aagttgtcat ttactgatat agccaacaca tcaggaactt ataccatcta
     1741 taaagctaac gcagtgatta ctgtcgatga cgctgccttt aattatgatg cccaaagtca
     1801 ctcgatttcg gttggaaatc tccacgttac cggtgttgtc ttaggcgaat cattggatta
     1861 taccctaaca gttaacagcc ggacagatat tggatcacaa atagtaacga ttagtttagg
     1921 cgaaggaagt gtaaacaata attatacgat tatcgcaaca gataccgcta agttgacgat
     1981 caatccgact ctcttgcttc cttcgacagg tgggataggt ctactgccct ttgtcttttt
     2041 aggactgatc tttatttttt ctggcttctt ttattttatc catcgtaaga aaaaagcagg
     2101 tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata tatttgctgt tctctttttt
     2161 atcctactgg tcttttttat aggttctccg actgtgtcag ctagagaatc acctattaca
     2221 tttactttgc ataatcgaat gttcgaaagc gataagatgc caagtatcag tcaaaacgac
     2281 ggaataaata tcccggataa ctttcaagga gtaaaagggt tgaacggcgt tactttcgct
     2341 atatatgatg tctcggatga attttacaag ctgcggtcag aaggaagtag tgtggaagac
     2401 gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa tactggcaga aaatgtgaca
     2461 aaaacagtag acgaggaaga gggcattgct tcttttagtg catcggacaa agatgagcaa
     2521 ggtcgtgacg ctgtttaccg atttgcggaa ataaagactt cagattaagt aaaagaaaaa
     2581 tcagcaccat ttgtcgttgt gcttcctgtg acagattcat ctaatcataa attgacgacc
     2641 atccacttgt atccaaagag cgaacaaaaa atacctgctg cactcacatt aacaaaaaca
     2701 gtagagaata agcagacaga ttttgcagat ggggataaag ttccttatct catcaccgta
     2761 acaattcctg agaacatcaa cgagatagga acctatacga tcaaagatac agctgatcct
     2821 cagttatggc tagaaccaga aacgatcgat tttttgattg gtggagacaa gattcataca
     2881 tttcacacac aaaaaacaaa acatggtttt gtcttatcag attctggaaa agatcttagt
     2941 tcgtttgctg ggaaaaaatt aacgatccgt taccaaatga cattgaaaga aaacagtgaa
     3001 aaaacgacat ttgataacga tgtacgtttg acgactgaca atcaaacagt agaaacacat
     3061 cttgccatcc aaacaggagg caaacaattt gtcaaagtgg atcggcaaac gaaacaaaaa
     3121 ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat acttagcgca agaaaacggg
     3181 ataaatcatt gggtagccaa agatgattca agagcaacga tttttacttc cagtaaagat
     3241 ggaacatttt ctgtccatgg attgtctttt ggaagttacg aactagtgga gattaaaccg
     3301 cctaaaggat atatattgag tcaatcaacc attctctttg aagttgaaaa aggcagttat
     3361 gatccgcttc atcctatacc attggaaatc atcaacgaac caaaagcaaa tccgacgaat
     3421 aatggaaaaa cgccagttca gatgcagaca aatggtccat caaatgaaaa aaatggagga
     3481 agctttccaa aaacaagcga agagatgctt ggaggtttct caattttagg gttgttgcta
     3541 gtcctttcaa cagggacagc ttggttttat aaaaaacaac aaaaaggaaa caataggagg
     3601 gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt tttaggaact gctatcatgc
     3661 tgcttccttt cgttctcggc ctaggaactg cagaggtttc taggccgtat cacctacacc
     3721 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     3781 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     3841 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     3901 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     3961 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     4021 aacgtcgcca aatatcgtgt taagcttacc cttttataac ccgaattcat cagaccctga
     4081 caaaccttta gacacgattc acctatatgc taaaaatgtc atcaaagaaa gcaatatcaa
     4141 aatccagaaa gctgggaact tttcaggggt agataagtta gcaggggcgc aattccagat
     4201 caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa aacactggtg tcgctctcta
     4261 tgatcctgct gagtcagcgt tgacattcac aactgatact gaaggatcca tcagtgtcaa
     4321 tggattaata gacggcaatt atactttagt tgaaacaaaa gcaccagatg gttatcaatt
     4381 aaccaatcca caaaaagaaa ctacttttat agtaaaaaac ggaaaattgc aacaaacaac
     4441 cggtatcatc catggcgcag atgaagccaa tcttgggaat ccaaacggtg tagtcaatcg
     4501 ggaaaataca actacggata acacgattac tgtagaaaat gtccaaaact acggtgattt
     4561 ccacttcatc aaacaagatg tgaacacgca agaaaaatta gccgatgcag aattttctat
     4621 tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa atagcaaatc ttgcctcagg
     4681 agaataccca tatgcttggt cagatgaagt cacaggttct gactgggaag aagtgaaatt
     4741 gatttctgat tcagaaggga aattttcaat cgaagggctg aaacaagggg catactgcct
     4801 tcaagaaaca aaagcaccag aaggctacgc attacctaag aatgaagctg cgtacactga
     4861 atttacagta gatgatgatc caacaacttc tgatgaaaca acgatcgata accaacctaa
     4921 aggaatcttg ccttcaactg gtggtatggg gatcatcgta ttcatcttgg taggtactgc
     4981 tttagttgga ggagcagtga tctacttcaa aaaacgccat caagaaacag aagcttaaac
     5041 tattggaaac aaaaatcata cgataaataa acgatagtga ttcaaatggg gagaaaggtt
     5101 gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg gcaa
//
LOCUS       DAFBYI010000142         5101 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFBYI010000142 DAFBYI010000000
VERSION     DAFBYI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.145"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(203..1165)
                     /locus_tag="LLD04_002518"
     CDS             complement(203..1165)
                     /locus_tag="LLD04_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5649408.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1167..2606)
                     /locus_tag="LLD04_002519"
     CDS             complement(1167..2606)
                     /locus_tag="LLD04_002519"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK5649409.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2791..4737
                     /locus_tag="LLD04_002520"
     CDS             2791..4737
                     /locus_tag="LLD04_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK5649410.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5001..>5101
                     /locus_tag="LLD04_002521"
     CDS             5001..>5101
                     /locus_tag="LLD04_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5649411.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTT"
BASE COUNT         1658 a          850 c          837 g         1756 t
ORIGIN      
        1 caatttcgta aaacaaccgt aacagttgtg taaaaatatc attttagagc atgaaaaaag
       61 gagtggattt ctccactcca aaaaagtcta acttttgggg gtcactacaa gttgacaact
      121 cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg aattttatag
      181 acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag attggtaaaa
      241 tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca acaataagac
      301 ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat ttctcaataa
      361 aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt ttaagttgat
      421 gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata ccatctggtt
      481 tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac agattattag
      541 gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca cgtaattctg
      601 tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa ccattttttt
      661 gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca atggaaacat
      721 taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca taatcagcaa
      781 ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca agcatttcaa
      841 tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt ttatatccat
      901 gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata ttactaatat
      961 taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt gctaaattat
     1021 ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt ttttctgata
     1081 aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca gctaattcag
     1141 cgacatcagt taattttgct accattttaa tattttaatt caaagtattt tccagtaggt
     1201 tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg actagtaaac
     1261 actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa aatgttagca
     1321 gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt ggcatattga
     1381 atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac aatcaagcta
     1441 agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt tggaggaaaa
     1501 gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg ttcagaacta
     1561 gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc ttttattgaa
     1621 agttctttta caagactcat tgctccctga taatcatact tatcagttgg ataatcgata
     1681 tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt gaatccttga
     1741 gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt
     1801 actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat attcccataa
     1861 tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt tttgtctaca
     1921 aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa atcaaggttg
     1981 ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt gataaagcct
     2041 gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata attaaacatt
     2101 tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac attctcacct
     2161 ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc acgttcccaa
     2221 ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat ttcatatgca
     2281 gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt
     2341 acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa cccatgagca
     2401 gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca
     2461 ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg ccaaggtgat
     2521 cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga ataagcacga
     2581 tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt ttacaactag
     2641 tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata aactatatga
     2701 caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag tgaaccgttt
     2761 tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc ttccgacgta
     2821 atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac acgtcttcgt
     2881 ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg
     2941 aaaagcacat ttaaaattaa tggacaatat caagtgatta tcggtgcagg tgatgttaat
     3001 ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac agatgattta
     3061 aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat taaattatta
     3121 tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt aatggcatta
     3181 caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga aatgtatcct
     3241 gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt catgttctta
     3301 ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt tcttggtgct
     3361 gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata ttggaatatt
     3421 tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt tttagcagct
     3481 gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc atctattgac
     3541 tttacattca cgccattatt atcagtaatg attactggat ttttgacttt cacagttatt
     3601 ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg gctatataac
     3661 actactggat ttattggaat gggtattttt ggaggaacat attcacttat agtcatgact
     3721 ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg gacaaatggt
     3781 atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct
     3841 gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct tgcttcctct
     3901 gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt taacttaaaa
     3961 taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc agtagcagga
     4021 ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt tctttcaatt
     4081 aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt agttgctttt
     4141 gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc agaagaaatt
     4201 gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat atctactgat
     4261 gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat ttctggtaaa
     4321 gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat gggaaaaggg
     4381 attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac tattcaactc
     4441 acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga agttctaatt
     4501 catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa agttactact
     4561 aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat gattgagaaa
     4621 gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta ttcagaagta
     4681 aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat caaataaata
     4741 tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag attaatcaaa
     4801 tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa aaattattag
     4861 aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt
     4921 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
     4981 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
     5041 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
     5101 c
//
LOCUS       DAFBYI010000143         4924 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFBYI010000143 DAFBYI010000000
VERSION     DAFBYI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.146"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..2168
                     /gene="mutS"
                     /locus_tag="LLD04_002522"
     CDS             <1..2168
                     /gene="mutS"
                     /locus_tag="LLD04_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5649412.1"
                     /translation="EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEME
                     ENAEFSFLTSELVDPLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSK
                     FNLELSQSIRTGKKHGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASL
                     LDSYFERIDLQSALTKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGID
                     QGQWQSLLSEMQPMDHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGK
                     KWLAELEAKERQETGIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERF
                     ITPELKELETQILEAEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFA
                     TISERYQYVRPELVSDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGP
                     NMSGKSTYMRQLALTVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVE
                     MMEANQALRYATPNSLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHE
                     LTVLEKELPQLKNVHVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERA
                     ADILHTLENGDNGHHTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSL
                     FSELSENETEVIESLKQINLLEMTPMDALNTLYELQKQL"
     gene            2186..4294
                     /gene="mutL"
                     /locus_tag="LLD04_002523"
     CDS             2186..4294
                     /gene="mutL"
                     /locus_tag="LLD04_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK5649413.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            4316..4876
                     /gene="maf"
                     /locus_tag="LLD04_002524"
     CDS             4316..4876
                     /gene="maf"
                     /locus_tag="LLD04_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK5649414.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         1722 a          904 c         1098 g         1200 t
ORIGIN      
        1 aagaagctgt tttaaatgaa gcctctgcat tgcagacaaa agaactcgta ttaggcagtg
       61 agatcactga tacccttcgt gaacagctaa gcgggcgttt agggctagtc ttttctcaac
      121 aaaatgagat ggaagaaaac gcggaattta gctttttgac aagcgaacta gtcgatccgc
      181 tgaaaaaaga agcaaccggc aaactattga cttatttatc ggtgacacaa aagcggagtt
      241 tagctcatat ccaaaaagca gtagaatacc aaccagatca ttttttgaaa atggattatt
      301 attcaaaatt caacctagaa ttaagccaat ctattcggac tgggaaaaag cacggcactc
      361 ttctatggtt gttagatgaa acaaaaacag caatgggtgg ccgtttacta aaacaatggc
      421 tagaccgtcc gcttatccag gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt
      481 tgttagacag ctattttgaa cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc
      541 tggaacgcct agcaggtcga gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc
      601 tgaaaaattc tttagaacaa gtgccaatga tcagagggtt gattgaagga atcgatcaag
      661 gtcaatggca aagtctgctt tcagaaatgc agccgatgga ccatctcgtc caattgatcg
      721 accaagctat ccaagatgat ccgccgttac aaataacgga aggaaacatc atcaaagatg
      781 gattcaatga gcagctcgat atgtaccgtt ctgctatgag aaacggaaag aaatggttgg
      841 cagaacttga agcaaaagag cgtcaagaaa caggaatcaa aaacttaaaa atcggattta
      901 accgcgtttt cggttattat atagaaatca caaaagcaaa tcttggaaat gcagaactgg
      961 agaagtatga acgaaaacaa actttagcaa atgcagagcg tttcatcaca ccagaactaa
     1021 aagaattgga gacacaaatc ctcgaagcag aagaaaaatc tgttgattta gaatatcagt
     1081 tgtttttagc tgtgagagaa gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa
     1141 aagcaatcag tgcagtagat gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt
     1201 atgttcggcc agaacttgtc agtgacaagc atcagcttct gatcaaagac ggacgacatc
     1261 ctgtagtaga aaaagtatta ggacatcagg aatatatccc taacagcgta gaaatggcag
     1321 aagacgaaat gatcttgctg attacaggac cgaatatgtc tggtaaaagt acgtatatgc
     1381 gacagttggc attaactgtt ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag
     1441 cagttttacc tatttttgat cggattttta cccgaattgg tgcaagtgac gacttaattg
     1501 ctggacaaag tacttttatg gtagagatga tggaagccaa tcaagccctg cgttatgcta
     1561 cgccaaacag tttgattttg tttgacgaac tagggcgagg aacagcaacc tatgacggaa
     1621 tggcgttggc ccaagcaatc atcgaatata tccatcgtca tgtacaagca aaaacactct
     1681 tttctactca ttatcatgaa ctgacggtac ttgagaaaga acttccgcag cttaaaaatg
     1741 tccatgttgg agccgttgaa aaagacggag aagttgtctt cttgcataaa atgatggatg
     1801 gacctgcgga taaaagctac ggcatacatg tggcaaagat cgcaggattg ccttccaatc
     1861 tactagaacg agcagcagat atcctgcata cgctagaaaa tggcgataac ggacaccata
     1921 ctgttccagc tgaagtagaa gagaaaacta aaacagccaa acaggagcaa ataaaaccac
     1981 ctgcagaaaa agtaaatcaa gaccatatac aagaccgtgt aaaagaagaa aaccaacagc
     2041 tttctttatt cagtgaatta tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa
     2101 taaatttact agaaatgacg ccaatggatg cactaaacac attatatgaa ttacaaaaac
     2161 aactgtagga agaaggcgga tatcgatggc caaaatccaa gaattatctg aacgtttagc
     2221 taatcagatc gctgcgggag aagtagtgga gcgaccagct tccgttgtga aagaattagt
     2281 ggaaaatgca attgacgccg gcagtactca gattgatatc ttagttgaag aagcgggact
     2341 gcggacgatc caagtgattg ataacggaga aggcattttg gcagacgacg tagagaatgc
     2401 cttcaagcgg catgcgacaa gtaagatcca taatcgcgac gatctttttc gcatacgaac
     2461 gctgggcttt cgaggggaag ctttgccaag tattgcctct gtttctgaaa tgactctgga
     2521 aacggcgaat caggaagaaa aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat
     2581 tgagcatcga ccagcaccac tgcgtcaagg aacgaagatt accgtcagca atctgttttt
     2641 caatacgcca gcccgcttga aatacgtgaa aacactgcac acagaattag cgaacatcgg
     2701 ggacatcgtc aatcgactgg cattgagtca tccatcgatt gcttttcgtt tagtacatga
     2761 tggaaacaaa atgctggcaa ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat
     2821 ctacggatta tctaccgcaa aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga
     2881 gttatttggc tatgtttcat tgcctgaggt gactcgtgcc agtaggaatt acttatcgac
     2941 gatcatcaat ggacgtttta tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta
     3001 tgggtcaaaa ttaatggtcg gtcgtttccc tattgctgta ttggaaatca aaatggaccc
     3061 gcttttagta gatgtaaatg ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa
     3121 agaactaaca gagctaatta gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat
     3181 tccaagtgca gcggataatc tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga
     3241 acaaatcgaa atcgatttgt cctacgcttt tgaagaagaa aaacctaaaa agccaagtag
     3301 tttgaatttt gatcgagaga caggtacctt tttcgtagaa gaaccaaaga acacatttgt
     3361 tgacagaatt gttgataagt ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga
     3421 aaacaccact tatccagaaa aaacagcgaa aacggtggat aagtttggtg aaaatgttga
     3481 taactttgta gaaaaaacag tggaaaaatc tgaaaagaaa gaatttcccg ttgtggataa
     3541 catggagaaa acaaatgaag aacaagcaga taaatccgta gaagaggatg aaaagattta
     3601 cgaggaagaa ttgacactcc atcctgaatt tgatttcact caaggaaacg acaaaacatt
     3661 agaacaagcc atcaagaaat tagaaacgga aaaaccagta caacgttttc ctcagttgga
     3721 atattttgga cagatgcacg gaacgtatct ttttgcccaa agtagcgatg ggttgtacat
     3781 cattgatcag catgctgccc aagagcgcat taagtatgaa tatttccggg aaaaaattgg
     3841 ggaagtatct gatgatctgc aagaattgct tgttccattt gtgttagact atccaaacag
     3901 tgatgctttg aaattaaaag agcaaaaaca cttgctagaa gatgcaggga tttatttaga
     3961 agaatttggt caaaacagct ttattgtacg ggcccatcct acttggtatc ccaatggaga
     4021 agaagaatcg attattcggg agatgatcga tatgctgctg acaactggaa gtgtgagtgt
     4081 aaaaaaattc cgtgaagcaa cagcaatcat gatgagttgc aaacgatcga ttaaagccaa
     4141 ccattatttg aatgaagcac aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa
     4201 tcctttcaat tgtccccatg gccgtccagt attgattcac tttaccaatt cggatatgga
     4261 aaggatgttc aaacgaatcc aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa
     4321 gattgtatta gcatcccaat caccaagaag aaaagagtta ttggctcata tcgtttcaga
     4381 attcgagatt gaacctgctg atatcgatga aacgccattt ccagacgaac agcccgtcga
     4441 ttatgttcgt agaatggctg aagaaaaagc acgaattgtc tgggaacaag atgaaaaaga
     4501 agaccattta gtgattgcca gtgacacgac tgttgttctc aatcaagaga tcatgggtaa
     4561 accagaagat ctggctgagg ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac
     4621 tgtatataca gctgttgttt taaaaacaaa ggaacgagaa gaacggatac tagcagaagc
     4681 acatgtcact ttttatcctt tgacagacga agagatcaaa cgctacttgg agacggggga
     4741 ttatgcggat aaggcagggg catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga
     4801 aatttctgga gattattaca gtatcgtagg atttccgatt ggtgctgtca atcaggcact
     4861 gaaaacattc atttaaacaa aaaagcggac tatctgattt tcgattgaaa atccatgata
     4921 gccc
//
LOCUS       DAFBYI010000144         4889 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFBYI010000144 DAFBYI010000000
VERSION     DAFBYI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.148"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(172..924)
                     /gene="xth"
                     /locus_tag="LLD04_002525"
     CDS             complement(172..924)
                     /gene="xth"
                     /locus_tag="LLD04_002525"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK5649415.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1037..1957
                     /locus_tag="LLD04_002526"
     CDS             1037..1957
                     /locus_tag="LLD04_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5649416.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2030..2965
                     /gene="murB"
                     /locus_tag="LLD04_002527"
     CDS             2030..2965
                     /gene="murB"
                     /locus_tag="LLD04_002527"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK5649417.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3263..4741
                     /locus_tag="LLD04_002528"
     CDS             3263..4741
                     /locus_tag="LLD04_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649418.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1720 a          779 c          936 g         1454 t
ORIGIN      
        1 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaatctct cgataaacag
       61 tgttcaaaag ccagtttctc ggtattatgc caaatttatg caaaatgtgg aaagccattt
      121 tcacaaaatt tttcttaata ctcgaaacta aacgactttt tcacaacctc tttatttcaa
      181 atctaattct actggacaat gatcactgcc cataatgtcc gtatggatct ttgcatcagc
      241 tagtcctggc tctaaatctt tgctcgtcaa aaagtaatcg atccgccaac cagcattgtt
      301 tttccttgaa ttgaatcggt agttccacca agagtagaca ccttcctggg ttggataaaa
      361 ataacggaat gtatcgataa acccgttatc caacagtcga gaaagagccg ctctttcttc
      421 tggagtgaat ccagcatttt tttgattcgt tttccagttt ttcaaatcaa tcttttgatg
      481 agcgacattt agatccccac acacgatgac tggtttttgt ttttttagat tttccaaata
      541 attataaaag gcttcttccc attctaatcg gtaatctaaa cgttttaatt cgctttgaga
      601 atttggcgtg taacaggtta caagaaagaa atcggaatat tccaaagtaa tcagacggcc
      661 ttctgtatca tggatatcga tacccattcc gtaagtggca ttcaatgctg gttttttagc
      721 aaaaatcgct gtgccggaat agccttttcg ttccgcgtaa ttccaatatt gataataacc
      781 tggaagatct aaatcaattt gtcctgcttg gagtttcgtt tcctgcagac agaaaaaatc
      841 ggcgtctaac tcatgaaaaa cttctaaaaa atttttattt acgatggcac gaagcccgtt
      901 tacgttccaa gagataaatt tcaataaaag cacactcctt cgaattaatt tccttacctt
      961 tgcgatacta ttgtacctca ttttagtaaa aaatggtgta agatgaaagt aactaaatga
     1021 tccaggagga aagtagatga agattggtgt aatcggtgta gggaatatcg cagaaaaagc
     1081 atatttacca acttatgcga aaaaacaagg aacacttgat ttttattttg caacaagaaa
     1141 taaagaaaca aaagaaagaa ttcaaaaaga gtacgggttc caacatctat atgaaacgat
     1201 cgatgagctt ttagaagaaa aaatcgaagc atgtatgatc cacgcagcaa caaaagtaca
     1261 ttatgaatta gccaaaaaat gtctagaaaa tggtatccat gtttatattg acaaaccact
     1321 gagtgtccat ctaaatgaag ttcgtgaatt gcaagaatta gctgataaga accaaataat
     1381 tttgatgatc ggtttcaacc ggcgctttgc tcctatggta gaagaattga aacgtatccc
     1441 agataagcgg ttgatccacc tgcaaaaaaa tcgaatagcg gcaaaagaaa cgacagagtt
     1501 cgtcctttat gatttgtttt tacatcttat tgatacagct gtgtatttat tagatgaacc
     1561 cattttgaga acaaaatacc agataagaga aacaaaggaa tttttggaat atgccatttt
     1621 acaattagaa acacaaaatc aaacggctat tttaacgatg gatttagcaa gtggcgctaa
     1681 tatagaaaaa tatcaagtaa caagtaaaaa tggtacttat gaagtaaacg atctgaccca
     1741 gcttgtcatt caagatgctg atggaaaaaa agtgaaagaa tttggcgact ggacaaacac
     1801 gctagtcaaa cgcgggtttg agccaatggt agaggccttc ttttctcaga tccgtttagg
     1861 taaatcggaa acagaattgc ttaaacaaaa agaagttgta agaagtcatg aattatgtga
     1921 ggaagtgctt aaagatcact accgtcatca actttgaaca agactggcaa gattgtatta
     1981 agcgtgatag aataatgagg acgtttgaga aaagaaagga aaatcctcag tgaataaaga
     2041 agaaatagtt gaaagcttag ccacgattac tatattgttt gatgaaccat tgatgaacta
     2101 tacgtttacg aaaacaggtg gaccagctga tgtccttgca ttcccgaaaa aacaagaaga
     2161 agtaaagcaa atcatcgatt attgccgaat ccatgatata ccttggatgg tattaggaaa
     2221 tgcaagcaat ctgatcgttc aagatggcgg gattcgcggt gtagtcatca tgctgactga
     2281 aatgaaacag attcatgtca aaggaacgat ggtcattgcg gaagcaggtg cctcactgat
     2341 tgatacgact tatgcagctt tggcagaatc attgactgga tttgaatttg cttgcggcat
     2401 cccgggaagt gtcggtggag cggtttatat gaatgcaggt gcgtatggcg gagagatcaa
     2461 agatgttttt gctgaagtgg atcttttgct tgaagatgga acattgaaaa ctttgacaaa
     2521 agacgaaatg gtattttctt atcgtcacag taaagtccaa gaattacgtg ctatcgtatt
     2581 ggaagctcga ttcagtttgc aaacagggga ttatgaagca atcaaagcaa gaatggatga
     2641 gttgaccgaa ttgcgtcaat caaaacagcc attagaatac ccttcatgcg gaagtgtctt
     2701 taagcgccca gtaggacact atacaggtca gctgatccaa caagcaggat tgcaaggcct
     2761 gaaatggggc ggtgcacaag tctctgaaaa acatgctgga ttcatcgtca atatcgatca
     2821 tgcgacagcg acagattata ttgaattgat tgcgcacatc caagaagtaa tcaaagaaaa
     2881 atttgatgtg tctttagaaa cagaagtgcg gatcattgga gaaaaacaag caatatcaga
     2941 aacaagcaga agtttagcca attaataagt aatggaactt aaaataagta aaagagcagg
     3001 acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa tcttgtacat
     3061 tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc attttttgca
     3121 atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag cataatccat
     3181 cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta attggtatga
     3241 ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg aagatagaga
     3301 aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag tgctgcctaa
     3361 gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca tagaattaaa
     3421 taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag aagcagagat
     3481 ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct actttcaacg
     3541 atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga tcgaaaaatt
     3601 tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag aattagtctc
     3661 ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg aagaaataaa
     3721 catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt ctatgccatt
     3781 tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat atctgttcaa
     3841 tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta ttagtttgtt
     3901 gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat actttaagag
     3961 ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc atatccctaa
     4021 agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg aagaagaatt
     4081 tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc acatgattag
     4141 tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa tctctgaaga
     4201 agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa
     4261 ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac agtttttctt
     4321 cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga ataaacgcta
     4381 tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc tctttttatt
     4441 ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta ttgatttttc
     4501 gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta aaaattacaa
     4561 tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg attgtttact
     4621 tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag atgactggga
     4681 atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa aaatcagcta
     4741 gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt ttttttcaaa
     4801 gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag
     4861 tacataagtt attattccga ttttcttga
//
LOCUS       DAFBYI010000145         4812 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFBYI010000145 DAFBYI010000000
VERSION     DAFBYI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.149"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..89
                     /locus_tag="LLD04_002529"
     CDS             <1..89
                     /locus_tag="LLD04_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK5649419.1"
                     /translation="LYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            89..340
                     /locus_tag="LLD04_002530"
     CDS             89..340
                     /locus_tag="LLD04_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649420.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            356..772
                     /locus_tag="LLD04_002531"
     CDS             356..772
                     /locus_tag="LLD04_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649421.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            754..1026
                     /locus_tag="LLD04_002532"
     CDS             754..1026
                     /locus_tag="LLD04_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649422.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            1193..1594
                     /gene="tnpA"
                     /locus_tag="LLD04_002533"
     CDS             1193..1594
                     /gene="tnpA"
                     /locus_tag="LLD04_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK5649423.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1606..2751
                     /locus_tag="LLD04_002534"
     CDS             1606..2751
                     /locus_tag="LLD04_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5649424.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2989..3252
                     /locus_tag="LLD04_002535"
     CDS             2989..3252
                     /locus_tag="LLD04_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBK5649425.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            3246..3596
                     /locus_tag="LLD04_002536"
     CDS             3246..3596
                     /locus_tag="LLD04_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5649426.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3620..4234)
                     /locus_tag="LLD04_002537"
     CDS             complement(3620..4234)
                     /locus_tag="LLD04_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649427.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4684..>4812
                     /locus_tag="LLD04_002538"
     CDS             4684..>4812
                     /locus_tag="LLD04_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBK5649428.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSR"
BASE COUNT         1750 a          717 c          885 g         1460 t
ORIGIN      
        1 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt
       61 tgattgatga gaaaggaaag aaacgttgat gaaaacacaa cacaaattat tgactgtaaa
      121 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagcttt
      181 acttattccg cggtactgga cgacagaaac ccctttagta gaggatgcac cgactatgct
      241 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac
      301 ttatgaaaac gaatattggt taaccgaatt agagaactaa aaaggagaaa aaaatatggc
      361 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
      421 ggatatgaca ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt
      481 gtcggtagct gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta
      541 ttatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
      601 atgcttggaa gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga
      661 actaacgtat gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt
      721 agattatttt gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg
      781 attcaaaaag actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa
      841 cagttatatt tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta
      901 gatgtgtata aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa
      961 acagaaaaat acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg
     1021 gactagccga gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta
     1081 gctttagcta ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa
     1141 taattgattt tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga
     1201 aattcattat ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa
     1261 atatcgacat gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca
     1321 aattgctgat gataataata taaaaattat agaaatagaa tcagataaag accatattca
     1381 tttactcatt gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg
     1441 cgcatccgct agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg
     1501 acatctttgg aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat
     1561 tagaaaatat atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg
     1621 gcttataaat ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt
     1681 ggctgttcaa gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact
     1741 actggaaaag gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat
     1801 gcagagacaa tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat
     1861 ctttctgatg ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt
     1921 aaaaaacatc ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga
     1981 aacaatttcg taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc
     2041 aaagggcgaa tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc
     2101 atcctgtgtg aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac
     2161 cttggtataa ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc
     2221 acgtcaaaaa tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta
     2281 ctagctaaaa agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga
     2341 aaagtagcac gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg
     2401 agcacagaga ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt
     2461 atgttgcgta atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg
     2521 actaagttac aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg
     2581 tttccatcta gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa
     2641 attcgagaat ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt
     2701 atcaatattt tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca
     2761 tcaaaaaccg taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa
     2821 gaaatacgct tgcaagtatg ctctattccc aagaatctcg tgactttagt catgagaggt
     2881 tcaaggattc atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt
     2941 ttttcttatg tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta
     3001 cctataaaaa aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat
     3061 cttggaattc atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag
     3121 cggctaatta tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa
     3181 aattacactg atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat
     3241 gaaaaatggt gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat
     3301 ctggacacga acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg
     3361 attatagtaa cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat
     3421 atgtaaatat aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac
     3481 taacgacaat tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt
     3541 tagttgagga gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata
     3601 gaaacagcag catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata
     3661 cggtttgtct cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta
     3721 gatcctcaac gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt
     3781 atagcttagg acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct
     3841 gacgtcgttt agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca
     3901 aatctttaat gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac
     3961 ttgtgatgat cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt
     4021 cgtaatttct tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga
     4081 aatccagtgc ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa
     4141 tgaaaattct ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat
     4201 cgatggatga gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat
     4261 ttaaaaaaca ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct
     4321 agttagggta taccttatat agacaaaaac taatgatgga ggaaatagta tgagaatttt
     4381 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
     4441 tattaaagaa cggaactcca aaaaagtccg tactctaatt tgcagtctct cactctttta
     4501 gagtgagagt ttttattaga ttttcaaatt aattagtaaa gtgatagaat aaattgatca
     4561 tacaaatcaa aagaagaagc agggtgatag catacctatg cttctttttt tatgtattta
     4621 gctatttctt ctttacaata tcgaacatat gtttgtataa tgtgaaagaa tggagtgtta
     4681 tttatgatgg attattcaaa agagccagta aatgattact ttttaatcga tatgaaaagc
     4741 ttttacgcaa gtgtcgaatg tatagaaaga aatttagatc cattaacaac agaacttgtt
     4801 gttatgagtc ga
//
LOCUS       DAFBYI010000146         4811 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFBYI010000146 DAFBYI010000000
VERSION     DAFBYI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.150"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LLD04_002539"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LLD04_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK5649429.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="LLD04_002540"
     CDS             complement(3252..3770)
                     /locus_tag="LLD04_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK5649430.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          787 c          731 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccacttag c
//
LOCUS       DAFBYI010000147         4784 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFBYI010000147 DAFBYI010000000
VERSION     DAFBYI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.151"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            6..863
                     /locus_tag="LLD04_002541"
     CDS             6..863
                     /locus_tag="LLD04_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5649431.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="LLD04_002542"
     CDS             885..1394
                     /locus_tag="LLD04_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649432.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="LLD04_002543"
     CDS             1431..1832
                     /locus_tag="LLD04_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649433.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="LLD04_002544"
     CDS             1944..2297
                     /locus_tag="LLD04_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649434.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="LLD04_002545"
     CDS             2331..2774
                     /locus_tag="LLD04_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5649435.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="LLD04_002546"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="LLD04_002546"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5649436.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="LLD04_002547"
     CDS             3869..4582
                     /locus_tag="LLD04_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5649437.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1658 a          730 c         1081 g         1315 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tcca
//
LOCUS       DAFBYI010000148         4714 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFBYI010000148 DAFBYI010000000
VERSION     DAFBYI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.153"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(213..443)
                     /locus_tag="LLD04_002548"
     CDS             complement(213..443)
                     /locus_tag="LLD04_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK5649438.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="LLD04_002549"
     CDS             complement(673..1491)
                     /locus_tag="LLD04_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5649439.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="LLD04_002550"
     CDS             1652..2341
                     /locus_tag="LLD04_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5649440.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="LLD04_002551"
     CDS             2355..3857
                     /locus_tag="LLD04_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5649441.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="LLD04_002552"
     CDS             3870..4346
                     /locus_tag="LLD04_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5649442.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="LLD04_002553"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="LLD04_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          829 g         1622 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatataca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFBYI010000149         4684 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFBYI010000149 DAFBYI010000000
VERSION     DAFBYI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.154"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(12..482)
                     /locus_tag="LLD04_002554"
     CDS             complement(12..482)
                     /locus_tag="LLD04_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK5649443.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="LLD04_002555"
     CDS             complement(641..1099)
                     /locus_tag="LLD04_002555"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK5649444.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="LLD04_002556"
     CDS             complement(1270..2415)
                     /locus_tag="LLD04_002556"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK5649445.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="LLD04_002557"
     CDS             complement(2428..3345)
                     /locus_tag="LLD04_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK5649446.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="LLD04_002558"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="LLD04_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK5649447.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="LLD04_002559"
     CDS             4437..4607
                     /locus_tag="LLD04_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649448.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1461 a          849 c          881 g         1493 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tatt
//
LOCUS       DAFBYI010000150         4596 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFBYI010000150 DAFBYI010000000
VERSION     DAFBYI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.155"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(756..2042)
                     /locus_tag="LLD04_002560"
     CDS             complement(756..2042)
                     /locus_tag="LLD04_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5649449.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(2035..3303)
                     /locus_tag="LLD04_002561"
     CDS             complement(2035..3303)
                     /locus_tag="LLD04_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5649450.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3555..4016
                     /gene="mscL"
                     /locus_tag="LLD04_002562"
     CDS             3555..4016
                     /gene="mscL"
                     /locus_tag="LLD04_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK5649451.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(4075..4521)
                     /locus_tag="LLD04_002563"
     CDS             complement(4075..4521)
                     /locus_tag="LLD04_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649452.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1314 a          943 c          803 g         1536 t
ORIGIN      
        1 aaaaatgaca aacctgttcc gactgtcctc attaagggca atcaaaacaa cgttccatcg
       61 ttttttggtt acagaaaaaa taggtaacaa aaagatgatt cacttatcaa cgcctcgtcc
      121 acaagtcacg atattcctca aagatgagaa gctgtttttt gaaattgtta cttgtgactc
      181 agagttaaat gacaggctca cgacctcttt cttaaacggt gttcaatcag ctgaccttcg
      241 gtattagctc aacaaacggc aaaatatgag gaactatttt tccgtttgtt tctctaatac
      301 tcaggtcaaa acgctgattt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
      361 gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct gtttttactt ggctgttctt
      421 attacttgat gcagggcagc tttttcacaa cctcttggta cacggtgttc cagaagtagc
      481 ttcttcggca ataagccgaa atatcacaaa aatttgagaa gcaattttcg taaattcctt
      541 cttatttctt gaagctgacc acttctgtca caacctcttt gattacttcg tttgatttta
      601 ttttaatata agttgattaa aagagagcat gatttgtttt attttctcac taatttttcc
      661 aacacaacta agaataccat aaaagctgat tttggaggag gttcctacta caagtctaaa
      721 acgaaattta agacttttcc tcataaaata ctggtttatt ttggtctcat gtaaaactcg
      781 ctgaaggcct cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc
      841 gaatcgatga tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat
      901 tgattggcaa tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc
      961 ttcagcaaat cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt
     1021 tctttgactt tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag
     1081 tgaaattcac gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg
     1141 tacatcgcta gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt
     1201 gctgttttat agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata
     1261 ccaagtacaa atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct
     1321 tttgtgtttt cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct
     1381 tggttttccc gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa
     1441 accatattgc ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt
     1501 ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag
     1561 ttatttagta tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc
     1621 tgaccgatga ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca
     1681 aaatcgatca gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg
     1741 gttttcgtaa aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag
     1801 acatcaccat cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga
     1861 actttcacct tttctttttc accataggga ataaattcgt tatcgatcga accataattc
     1921 gtactgaaca aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa
     1981 ccgtttggca gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc
     2041 atttgcattt cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg
     2101 ttgtatctct ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt
     2161 tgtttccggc agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag
     2221 agaaagcaga tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat
     2281 ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt
     2341 cttttcatcg ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga
     2401 ggcataataa gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa
     2461 accgccaaat aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac
     2521 atttgtctga taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc
     2581 cttcacgata ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga
     2641 ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac
     2701 aaaaatgtcg atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc
     2761 agtgatcttg tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt
     2821 ttcaaaataa agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact
     2881 ttccaaatat agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg
     2941 atttttaata tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag
     3001 gtttggatca ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa
     3061 atttcctttt tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc
     3121 acttaacttt gtctgatccg gataattcaa actatttgtt tccaacatgc tgctgagaag
     3181 tgaacgtttc gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat
     3241 cgttttgtat tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac
     3301 catatttata tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct
     3361 gcctttttta cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc
     3421 taaatgatgt tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc
     3481 taaaagcgta tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga
     3541 agggagggtc attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg
     3601 atcttgctgt cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc
     3661 aaggcttgat cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt
     3721 tagaaggagc actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg
     3781 gtaacgttat tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg
     3841 tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag
     3901 aaactcctac tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg
     3961 cagaagacaa tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa
     4021 gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact
     4081 tgctacttgt tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga
     4141 gattgccttg aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac
     4201 gattggtttt tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct
     4261 tcctaaacca tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc
     4321 cgcttcttca tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt
     4381 atgccaagct tgtccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac
     4441 aaaaactgcg attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa
     4501 tttctttacc tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg
     4561 attcgcacac aaaaaagcaa acgaacctta cgaatc
//
LOCUS       DAFBYI010000151         4579 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFBYI010000151 DAFBYI010000000
VERSION     DAFBYI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.156"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(346..1299)
                     /locus_tag="LLD04_002564"
     CDS             complement(346..1299)
                     /locus_tag="LLD04_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5649453.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1322..2077)
                     /locus_tag="LLD04_002565"
     CDS             complement(1322..2077)
                     /locus_tag="LLD04_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5649454.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2074..3033)
                     /locus_tag="LLD04_002566"
     CDS             complement(2074..3033)
                     /locus_tag="LLD04_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5649455.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3026..3976)
                     /locus_tag="LLD04_002567"
     CDS             complement(3026..3976)
                     /locus_tag="LLD04_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5649456.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4126..4578
                     /locus_tag="LLD04_002568"
     CDS             4126..4578
                     /locus_tag="LLD04_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK5649457.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1492 a          963 c          776 g         1348 t
ORIGIN      
        1 tcgtttaaag aagagatcgg tacaaaataa aaatatctaa atttaagttt tatctaagtt
       61 tcgctttgta cctttgattc aaatcaaagg aggcgatctt gatgctgaaa aaatggatca
      121 cactattttt tgccactccc cattctagaa tcaacccgta tgatgtagag aatgaattca
      181 cgttgaaaaa agatttccca aataaataaa aaccatccgt aaaatcatca atacgatttt
      241 acggatggtt tttgataact tctttgagga taggctcttg ctatttaaga atgtaagaaa
      301 cattattgtt acatagtatg agagtttaat cctctgttat ttatgctatt taaatgcttg
      361 attcacatct tcgatcatca gtttcattga ttctaaaccg cccccgctta aataccagac
      421 atctggttca agggagatga tctgctgatt tttaccagca ttcgtttgtg ctaccagttc
      481 attggcagaa atatcatctt tgctgtcgtc tccaccaata gcttttgttc tgtctactac
      541 aaataagata tccggatttt tttccagcac atattcataa gaaacacttt gaccgtgagt
      601 ggatgcttcg atttgatcat ctgcttgttc aaagccaaat aaatcatgga tgaaaccgaa
      661 tctagaacca gatccgtagg ctgataattg cccttcattc acaagtgtga ctagtgcttt
      721 tttgtcagta gcttctgctt tctttttcgt ctgatcgatt gcactagata attcctctaa
      781 ttgagtttta gcttcttctt gcttaccaaa gatctcccct aaagcagtga cattttgctg
      841 gaaagattcc catggatttt tcgcatctaa agaaaggaaa atggtcggag caatttgact
      901 tagatctttt tgaaaatctc tttgccggcc agaaatgatg atcaggtctg gctgaagctg
      961 attgattttt tctaagtctg gttctttgat cccacctgct gattccactt ttttatagga
     1021 tgaaagatac tcaggaaggc tgcttgtagc tgctccaact accttatctc caacaccaag
     1081 agcatccatc gtatctaaag aaccattgtc gaatacgacg actttttcag gattctttgg
     1141 cacctcgatt tgatcgccat tactgtcttt tatcgttaat gccgtttcag cttgcgtact
     1201 gcttttcttt gtatctgcct gactggtgtt gtcactattt ccacatgctg ctaaaccaaa
     1261 tgatgctaga atcaagatac ctactactaa ttttttcatc tcttctctcc taatgttagt
     1321 tttagaaata aagacagaat ctgcgacctt caatctcaca aattcgtatg tccatttcgt
     1381 acaattcgtc cagtacatct ttttttatca tttcttgggt cgttccttgc gtgaataact
     1441 gcccatcttt cattgcaata atttcgtctg cataacttgc agcaaagttg atatcatgaa
     1501 gaacaataac aattgttttc ccgatttctt gtactaattt ttttaagatc tgcatgattt
     1561 gaaccgcaaa attcatatct agattgttca aaggttcatc cagtaaaata taatccgtgt
     1621 cttgcgctag caccattgca atatacgcac gttggagttg accgcctgaa agagtgtcaa
     1681 tcgattgatc cgctatttct tctaacccaa ggtgttccag tgattcctga acgatttcct
     1741 cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg tccaaattct actaattctt
     1801 tcacggtgat ttggacattt atttcattcg attgtcgtag gatcgataat tttttggcta
     1861 attcattgga tttccaagcc tttacttcgt tatggtctaa atagatttca cctgtatctt
     1921 ttggtatcaa acgactgatc atcgacaaca ccgttgactt tccagcacca tttggaccga
     1981 taaaagcagt caacttcttt tcaggaatcg ttaactgtac atctttaacc actgattttc
     2041 catggtacag tttattgacc ttttgtaaat caatcatagg atactcttcc tttcgcgaat
     2101 caattttgtg acaaaataaa ttcctccgac aaattcaacg acgacactca ctgttgtatt
     2161 ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc agaaacagca tagctaccag
     2221 aaagcttccg ataaatagct gaccatgacg ataagtagaa aagacacgat aactgatatt
     2281 tgcgacaata aaccctaaaa aagtaactgg acccacaaga gccgttgatg tccctgtcaa
     2341 aagactgaca caacataaaa tcaaaaattc aaaacgatct gtgtctatcc cgaggtttac
     2401 tgcttgttca tttccgagat ggaaaacgtc tagcacatgt cgatatcgaa ataacaggta
     2461 ccagcagccg ataatgatca aagaagcagg caaaagatac tgactctgga tattgccaaa
     2521 gcttgcaaat aatctgcctt gaagcaaatc atattcattc ggatccatga tgacttgtaa
     2581 aaaggtactg acactaccaa aaagcgtacc taaaatcatc cctgacatca gtaataaaaa
     2641 aagattcgct ctcatttttt tcaaaagaaa gaaaaataac aatagactca gcccgaccat
     2701 cagcccaacg ttcaataaga aaaaaactgt acctgtctgc tgaagcatcg tcactcctcc
     2761 gataaagaag aaaaggaccg tttggacaag gacatataac gactcaagtc ccaatactga
     2821 aggagtcaaa aactggttat gcgtaattgt ctgaaagctg attgtggcac tgctgacagc
     2881 tagcgagaca aggataaatg caaaaatttt cttactcctc aatgcccatg caaaatgcca
     2941 gtttccatat gtatggatcg tcaagtacat tcctgtaaca aataaaacaa gaacaagcat
     3001 accaataaaa atagatggct tttttttaag catatcgtcg cccccttatc agaagataaa
     3061 ggaagatagc cccaccgatg attccaacga tcacactgac tggtatttca taaggaaaaa
     3121 tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa actacctgct aaagcagtta
     3181 cgccaattgt tttgtgcact tgatctccat atattctaga gacaagattc ggaatgatca
     3241 ctccgataaa aggaatgctt cctaccatga tcaacatgac tgcactagcc aatgatacta
     3301 agccaatgcc taaaaactgc atcttctggt aatctattcc caaatttact gcaaacgatt
     3361 cgcccagacc aatgatcgta aatcggtaag caaatagcca aaccaagagc aacaatggaa
     3421 caacagcata caataactca taatttcctc gtatcacagt cgcaaaattt ccttgaagcc
     3481 atgaagtcat attttgtacc aactggaatt gataagcaaa aaaagtagca actgaaccaa
     3541 tgatattccc taacatgact ccaatcaacg gaagcatcaa ttgatttttc tgggggaaca
     3601 atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc aaagcaaaat gctgtcaagg
     3661 atcggacaat caaggatgca ccaggtagga aaatcatagc cactaaaata cctaaacgcg
     3721 cactatccat cgtgcccgca gtcgttggtg aaacaaattt attttgggtc aaatgttgca
     3781 tgatcaaccc acaaatacta ctggtcgctc ccacaaggat caaacaaatc gttcgtggca
     3841 aacgagtcac tagcaagatt tgtaagcttt gcccatctaa ctggagcagt tctgacaatt
     3901 gtacattcga tactccgatg aacaatgaaa tactacttaa aataactaat aatgctacta
     3961 gtcgtccgta gcgcatccaa tccacctttc taattgagaa tgattttcat tcttatttta
     4021 agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt ttaggtatta ttgggaatat
     4081 gctataatcg taagaaagac aaagagaaga aggagggtat tttttatggc acaagatgat
     4141 gtaaaaagcc atttggataa gggactttat ggtacacctt tagtgaaccc tgaagaacag
     4201 cataaatatt tagggacatt cagggaacgc tgctatctta gtatgactgt tgctgaaatg
     4261 atccaaccaa aaaacaaaga aaacttttta aaagaactca aaaaacatcc agatgcttcc
     4321 atactcttga atggggctct acccctttct gtccaaaacc aatatatcca gctagcaacg
     4381 aaaaacaata gccgatttac tgtagtcaac gattacgtca ctgcaaatcc tgaggaattc
     4441 ggattgctct taacagcaaa agaagcagta aatgaacctg tgattgatat cgaaaaaaaa
     4501 tacccagaag aaaactcttc gccagttgaa aaagaaaaac caaaaaagaa aaaaagcttc
     4561 tgggatcgtt tattttaaa
//
LOCUS       DAFBYI010000152         4523 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFBYI010000152 DAFBYI010000000
VERSION     DAFBYI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.157"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            176..442
                     /locus_tag="LLD04_002569"
     CDS             176..442
                     /locus_tag="LLD04_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5649458.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            619..2577
                     /locus_tag="LLD04_002570"
     CDS             619..2577
                     /locus_tag="LLD04_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK5649459.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            2931..4232
                     /locus_tag="LLD04_002571"
     CDS             2931..4232
                     /locus_tag="LLD04_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5649460.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         1364 a          797 c          998 g         1364 t
ORIGIN      
        1 cgctgatttc acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct
       61 gaacactttt gttacaactt ctttttttct ggagtttctt ggagcatatt tttggagtcc
      121 ttctgaataa agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct
      181 gaatttttta tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt
      241 aggaaaggaa gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg
      301 gctcggcgaa tttttactag gaccactggg accggaactc ggcggttttt atttgatacc
      361 agcattattg ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag
      421 agaacgtcca aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg
      481 gataattaga agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca
      541 agcttaccta aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac
      601 aataccaaaa agatatcaat ggctggcctg cttgtagcga tgggagtcgt ctacggagac
      661 atcgggacaa gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca
      721 ggaatcaatc ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta
      781 ctgacaacaa tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga
      841 attttctcac tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg
      901 atcggaggag ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact
      961 gcgattgaag gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac
     1021 gtgatcgtca ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt
     1081 acagatattg tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc
     1141 gtaatgggat taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat
     1201 tatgctatcc agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat
     1261 attttcctgg ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa
     1321 cgaaatattc gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg
     1381 caggctgcgt ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg
     1441 aatccattct tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca
     1501 gtcgctgcag tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa
     1561 gcaatcaagt tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg
     1621 caaatgtata tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc
     1681 acattccgaa cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg
     1741 ttgatgacaa cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt
     1801 gcatatttag ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt
     1861 gtcaaattct tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg
     1921 atgtttattt gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg
     1981 agagattata tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag
     2041 acaaatgtgg tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc
     2101 tattcgattt tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa
     2161 gtgacggatg agccatatac gaaagaatat caagtagata tgatggacac tgattttatt
     2221 gtccaagtga atctgttctt aggcttcagg gtccaacagg aaatcaacgt atatgttcgc
     2281 caagtcatcc atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct
     2341 ctgacaccag ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc
     2401 aatgtgacaa ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa
     2461 aaaatcgcta cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa
     2521 actgttccgc ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag
     2581 tagcgctttg acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt
     2641 catgaaaaag ctgtactgca acaaacagta agaacgagca gataaaaaca gcttctcatg
     2701 tttttcatct gtttgggctt aatgccgcag atgcaagctt ttgaataccg tttttatcag
     2761 aggtcatgaa aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat
     2821 gtggctgaaa aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca
     2881 atctttttgt agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca
     2941 atttcattat tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg
     3001 ctttagtgaa aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg
     3061 ccaagctagc tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag
     3121 gaattacgct aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac
     3181 tagaaccagt gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg
     3241 cgctgggatt tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga
     3301 gctatagtat cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt
     3361 tttataaagt aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga
     3421 agctattagg tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga
     3481 cattagtcgc attgaattct tatgaaaaag gagagttgac caaagaagaa tatcattatt
     3541 tggaaaacgt gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt
     3601 ccatgcaagt attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac
     3661 aaggacatac tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca
     3721 ccttgccaga tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga
     3781 ttgaagagcc gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc
     3841 gaaaaaaggg aaagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag
     3901 tgacgattga agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg
     3961 acgaagcatt gatagcggaa cagtcagatg gctcttatat tatctccggc aaactaacat
     4021 tagatgattt ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta
     4081 ctacacttgc gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg
     4141 agattgcatc attccgtttc acggtattag aataccagca tgcacatatc gattatttca
     4201 aagtagagtc cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc
     4261 acagcctttt tcctacgatt agtcaagtaa aaggtgcaag aaaaatagct cgtaaacgaa
     4321 agcgacaaaa tcaaagcaca ccaaataaag ccccgttcgt aaaaaatttt cgatctgggc
     4381 aaagaagtgg tatactgtaa gtggtttagt taatggtcca ctgagaccgc gtacgcatag
     4441 tcgtactctg tatgtgccct aaccatagcg tgcatacatt tcagaagctt gttcatacaa
     4501 gcaactgtgg caaccttatc ttt
//
LOCUS       DAFBYI010000153         4514 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFBYI010000153 DAFBYI010000000
VERSION     DAFBYI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.158"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            544..1107
                     /locus_tag="LLD04_002572"
     CDS             544..1107
                     /locus_tag="LLD04_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649461.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="LLD04_002573"
     CDS             complement(1386..1673)
                     /locus_tag="LLD04_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5649462.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="LLD04_002574"
     CDS             complement(1663..1992)
                     /locus_tag="LLD04_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5649463.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="LLD04_002575"
     CDS             2297..2626
                     /locus_tag="LLD04_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5649464.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LLD04_002576"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LLD04_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5649465.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="LLD04_002577"
     CDS             complement(3551..4462)
                     /locus_tag="LLD04_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK5649466.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1496 a          755 c          672 g         1591 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaat
//
LOCUS       DAFBYI010000154         4430 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFBYI010000154 DAFBYI010000000
VERSION     DAFBYI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.159"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(543..2048)
                     /locus_tag="LLD04_002578"
     CDS             complement(543..2048)
                     /locus_tag="LLD04_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5649467.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LLD04_002579"
     CDS             complement(2045..2731)
                     /locus_tag="LLD04_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5649468.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LLD04_002580"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LLD04_002580"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK5649469.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DAFBYI010000155         4402 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFBYI010000155 DAFBYI010000000
VERSION     DAFBYI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.160"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            591..1280
                     /locus_tag="LLD04_002581"
     CDS             591..1280
                     /locus_tag="LLD04_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5649470.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LLD04_002582"
     CDS             1267..2475
                     /locus_tag="LLD04_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5649471.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LLD04_002583"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LLD04_002583"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK5649472.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1426 a          758 c          879 g         1339 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggttgcc gtagcaactt ttatatcata tcagagaggc
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttttcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta tt
//
LOCUS       DAFBYI010000156         4278 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFBYI010000156 DAFBYI010000000
VERSION     DAFBYI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.161"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            149..1072
                     /locus_tag="LLD04_002584"
     CDS             149..1072
                     /locus_tag="LLD04_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5649473.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1078..2331
                     /locus_tag="LLD04_002585"
     CDS             1078..2331
                     /locus_tag="LLD04_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5649474.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="LLD04_002586"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="LLD04_002586"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK5649475.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1473 a          674 c          928 g         1203 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcc
//
LOCUS       DAFBYI010000157         4277 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFBYI010000157 DAFBYI010000000
VERSION     DAFBYI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.162"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            38..1210
                     /locus_tag="LLD04_002587"
     CDS             38..1210
                     /locus_tag="LLD04_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5649476.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(1372..1827)
                     /locus_tag="LLD04_002588"
     CDS             complement(1372..1827)
                     /locus_tag="LLD04_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649477.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            2416..2838
                     /locus_tag="LLD04_002589"
     CDS             2416..2838
                     /locus_tag="LLD04_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649478.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(3004..4197)
                     /locus_tag="LLD04_002590"
     CDS             complement(3004..4197)
                     /locus_tag="LLD04_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5649479.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT         1417 a          806 c          783 g         1271 t
ORIGIN      
        1 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
       61 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
      121 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
      181 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
      241 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
      301 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
      361 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
      421 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
      481 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
      541 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
      601 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
      661 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
      721 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
      781 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
      841 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
      901 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
      961 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     1021 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     1081 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     1141 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     1201 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     1261 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     1321 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     1381 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     1441 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     1501 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     1561 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     1621 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     1681 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     1741 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     1801 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     1861 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     1921 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     1981 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     2041 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     2101 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     2161 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     2221 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     2281 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     2341 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     2401 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     2461 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     2521 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     2581 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     2641 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     2701 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     2761 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     2821 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     2881 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     2941 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     3001 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     3061 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     3121 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     3181 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     3241 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     3301 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     3361 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     3421 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     3481 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     3541 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     3601 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     3661 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     3721 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     3781 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     3841 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     3901 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     3961 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     4021 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     4081 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     4141 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     4201 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     4261 aaacaagact tttttat
//
LOCUS       DAFBYI010000158         4266 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFBYI010000158 DAFBYI010000000
VERSION     DAFBYI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.163"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1485
                     /locus_tag="LLD04_002591"
     CDS             <1..1485
                     /locus_tag="LLD04_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5649480.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            1589..1813
                     /locus_tag="LLD04_002592"
     CDS             1589..1813
                     /locus_tag="LLD04_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649481.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            1826..2329
                     /locus_tag="LLD04_002593"
     CDS             1826..2329
                     /locus_tag="LLD04_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5649482.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            2389..2778
                     /locus_tag="LLD04_002594"
     CDS             2389..2778
                     /locus_tag="LLD04_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5649483.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2765..>4266
                     /locus_tag="LLD04_002595"
     CDS             2765..>4266
                     /locus_tag="LLD04_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5649484.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1427 a          696 c          919 g         1224 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atcaggaact tacctatcca tatatcaatg
     1621 gtttttaacc aatgcccaat ccttagtctt actggcaatt gtggtcattg gtttgttttt
     1681 aggatttaag cgggagtttt ccaaactgat tggctttttg gtgattgctt tgattgcggt
     1741 cggattggtc tttaatgctt ctggggtgaa ggatgtctta ctcagtttgt ttaaccgaat
     1801 tgtcggtgcg taagggggaa gcacgatgga acagatgcgt gtttatattg cgaacctcgg
     1861 gaaatataac gaaggtgaat tggtgggggc gtggtttacc ccacctgtcg attttgatga
     1921 agtgaaagaa cgaattgggt taaatgacga atacgaggaa tatgcaattc atgattatga
     1981 acttcccttt gagattgatg agtatacgcc aattgaggaa attaatcggt tgtgtggctt
     2041 agcggaggaa cttgaaggaa cgccagttgg tgaagttgct tctgagattc aacatgcttt
     2101 cttcaattct tttgaggaga tggtagagca tgtggatgat attatctatt atccagattg
     2161 cgatgatatg agtgatgtag catatttctt gattgtagaa gctggtgatt ttggagaagt
     2221 accagaacac ttgaagacgt atattgattt tgatgcgtgt ggtagagact tggagattgg
     2281 aggcaattat ttggtaacga gtcgaggtgt ttttgagtat ccatattaac aaaattattg
     2341 aagggtagtt gatttctaaa ctatccttct ttttaaagga atgatttgat gaagaaaata
     2401 aaaagctaca ccagtatttg ggcagttgaa aaggtcattt atgcaattaa tgactttcaa
     2461 ctgccttttc ctgtgacttt taaccagatg gcttggtttg ttttatcctt gctgtttgtc
     2521 atcgtatttg cccatgtgcc tccactatct atgatagagg gggccttttt aaaatactta
     2581 ggtataccgg tggcagttac ttggttcatg tcgcaaaaaa cgtttgacgg taagaaacca
     2641 ttgggatttt tacgatcttt tattagctat cacttacgat ttaaggtcac tttcgcagga
     2701 aagaaagtca aagaacagaa gaaacgatgg gacgaaccca ttactctggt aaggagtgtg
     2761 aactatgtac ccgattaagt atattgaaaa taatcttgtc tttaatcaag agggagaatg
     2821 ttttgcctac tatgagttaa taccttacaa ttactccttt ttgtcacctg aacagaaata
     2881 tcaagtccac gataactttc gtcaattgat tgcgcaaaat cgtgaaggca agattcatgc
     2941 cttacaaatt gccacagaaa gtagtattcg tgccacgcaa gaacgatcaa aaaaggaaat
     3001 taccgggcga ttaacagaag tagcgaaaca acgaattgat ttacagacag aggcattagt
     3061 atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttca aactgattgc
     3121 gacagatgaa gaggtcaatc taaagaatct caagaaatct ttcttctccg gctttcaaga
     3181 atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga
     3241 aattcgccga tataccaagc ttgaaaaact aatggaaagt aaattggctc gaagatttaa
     3301 agtcagacga gtcacaccga gtgatctgat gtatttgatt gaacatatct atggcgaaaa
     3361 gggtacacca tttgaagaat atgaatttca gctaccaaag aaaaagctga agtcggaaac
     3421 tttggtgaaa cgttacgact tattacgtcc cagtcgttgt ttgattgaag aaaagccccg
     3481 gtacttatgc atggaacatg agaatcatga atcatatgta gcgtacttaa caattaatac
     3541 aattgtgggg gaaatggagt ttccctcctc agaacttttc tattaccagc aacaacaatt
     3601 tacattccca attgacacta gtatgaatgt ggagattgtg acgaacaaaa aggcacttgc
     3661 taccgttcgc aataagaaaa aggaattaaa agacttggac aaccatgcct atcaatctga
     3721 caatgaaaca aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac
     3781 cttggatcag tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga
     3841 atcagtagat gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa
     3901 tgtaaaacta gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc
     3961 tagtaagcga tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact
     4021 tggttttggc gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt
     4081 agatacgggg cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaagggttc
     4141 agttaccaat gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag
     4201 taacaatctc ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc
     4261 aaaagg
//
LOCUS       DAFBYI010000159         4224 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFBYI010000159 DAFBYI010000000
VERSION     DAFBYI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.164"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..345
                     /locus_tag="LLD04_002596"
     CDS             <1..345
                     /locus_tag="LLD04_002596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649485.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="LLD04_002597"
     CDS             426..1046
                     /locus_tag="LLD04_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649486.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="LLD04_002598"
     CDS             complement(1376..2602)
                     /locus_tag="LLD04_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5649487.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="LLD04_002599"
     CDS             complement(2674..2832)
                     /locus_tag="LLD04_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649488.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..>4224
                     /locus_tag="LLD04_002600"
     CDS             3520..>4224
                     /locus_tag="LLD04_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5649489.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1449 a          591 c          689 g         1495 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tatt
//
LOCUS       DAFBYI010000160         4217 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFBYI010000160 DAFBYI010000000
VERSION     DAFBYI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.165"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            626..3265
                     /locus_tag="LLD04_002601"
     CDS             626..3265
                     /locus_tag="LLD04_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5649490.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3433..4131)
                     /locus_tag="LLD04_002602"
     CDS             complement(3433..4131)
                     /locus_tag="LLD04_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5649491.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          806 c          911 g         1222 t
ORIGIN      
        1 tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat catttgacaa
       61 agagccagaa aaagccgagc tctcgataag ctcggctttc ttgtacctcg gaaggattca
      121 aataaacaga aatgattgta actttccatc atacatgcaa agcacggtgt tgaatttctt
      181 catgttaaac aaaaattttg ttttcgttac atcttcaaca tttgaaacat ctcttgttcc
      241 tattcaattt tcagacgttt acgttattca attgttacgt atcgcattac ttcttgtttc
      301 ggatacttcg tgatccaaac atccatacgc attcactatt tataggtttg tactctacac
      361 gcatgtctgg gtgaaacctg tacttctaac aactttattt gcttacactg ttctatcgag
      421 aaattcttct gtctatttga tggtatcctc cttccttaca tctatataat agcatccttt
      481 ggtagcgttt gcaaatataa gcactttaaa ttttacgctt tcttaacaaa taatcgataa
      541 gccctttatt tgaagtaaat aaaaaaagga tgttatagta aaataagtac ctttttttgt
      601 acgaagattt taaaggagga ataggatgga agaatacaag cagacaatag aagaagtaaa
      661 gaaggtaaca gaagcagatc cagatactgg gttgtcttca caagaagtgg atgaacggcg
      721 aaaaaagcag ggattgaata aattcgatga ggcgccgaaa gaatcgatga tcaaaaaatt
      781 ctttcgaagc ttatccgatt ttacaacgat catccttctt gtagcagcgg tgatttcctt
      841 ttataccgca tttgctacag aacatgggga tttatttgaa ggactgctga tcatcgctat
      901 cgttgtgatc aactcggtat tggcgattgt ccaagaagga aacgcagaaa aagcactgga
      961 atccttgcaa gatatgaata aacaaacggc aactgtcata cgagacggca aagtgaccac
     1021 tgttgagtca gagcaactgg tagtgggtga tattcttgtt ttagagtccg gtgatgctat
     1081 ttctgccgat gcccgtttga tcgaagcatc acaattacgt gtagaagaat cggcgttgac
     1141 tggagaaagt gaagcagttg aaaaagatgc agcatttatt gctgaagaag atgagtcatt
     1201 aggtgaccaa cttaatatgg tatttaaagg atgtacggtt gctgcaggtc gtgggaaagc
     1261 gattgtcact gccattggta tggctacaga aatgggtaag atcgcggatc ttttgaacga
     1321 aaatactatg caaaaaacac ctttacaggt ccgattgaat cagctaggca aacgaatcag
     1381 tatgatcgct ttagcggctg cggctttagt atttgtgatt ggtgaactgc aaggagaacc
     1441 actccttgag atgttcatga cttcgatttc cttggctgtt gcagctgtac cggaaacctt
     1501 gactgttatt gttacgttga cacttgcata cggtgtgcag aagatggcga ggaaacatgc
     1561 gatcatccga cagttgccag cagttgaaac gctaggaacc gctaatgtta tctgctcaga
     1621 caaaacagga acgctgacac aaaatgaaat gcgcgtccgc cgggtatgga gcaaagaaga
     1681 tgaagtaacg aatatcgaag attcaatgac gaacagcgcg atggaaatat tgaaaatggc
     1741 tgcactatgt acagatgtga cggtcgagca agaagatgac gaccttgtta tcaaaggtaa
     1801 tccaacagaa gtagccatcg taagggctgt tgaagagaat tatcatacaa aagcagaatt
     1861 agaagaaaaa tatccacgag taaacgagct tccttttgat tctgaacgaa aaatgatgac
     1921 aacagttcac caaatgggcg aaaaatacat ctcaatcacc aaaggagcat ttgatgttct
     1981 tgctccgcgc tttagttcag gcgatgtaga acaagcggcg atcgttaatg atcgctttgg
     2041 taaacgggca ctgagagtga ttgctgttgg ttatgctact tatgacgaag aaccgcaaga
     2101 tatttcttct gaagcactgg aaaaagactt acgtttgatt ggtctgatcg gaatgatcga
     2161 tccaccacgt ccagaaagta aaggagcaat caagcgagcg aaaaaggctg gcatcaaaac
     2221 agtcatgatc actggagatc atgtggtaac tgccagtgcc atagcgaaag aattaggtat
     2281 cttgaatgat ccgtcagaag cactctcagg atcagaactg catcaattat cagacgaaga
     2341 attagatgcg cgtgtcaaag ctctctctgt ctatgctcgg gtaacgccag aagacaagat
     2401 ccggatcgtc aaatcttggc aacgtacggg tgcagtcgta gcgatgactg gggatggtgt
     2461 gaatgatgca ccagcgttga aagcaagtaa cgttgggtgt gccatgggga tcacagggac
     2521 agatgtggcg caaagtgccg cagatatgat tttgacggat gacaactttg cgacgatcgt
     2581 agatgctgtt agtcaagggc gttcagttta tcagaatatt cgtaaagcaa tcaacttttt
     2641 actaagctgt aatatttcgg agatctttat tgttttgatt gcgatgctgt taggatgggg
     2701 agcaccattt acagctgtgc aactactgtt tgtcaatgta gttgctgatg gacttccagg
     2761 atttgcttta ggaagagagc cagcggaaca cggcatcatg gatcagccac ccattccgaa
     2821 aaatgaaggg atctttgctc gaggtttatt gcaaaaaatt gccataaatg caggggtctt
     2881 caccatcgta accttgtttg gttattattt aggtagttat gtcgatacta tcagcccatg
     2941 ggtagacgca agtcagcatg tcggtcaaac tgttgcattc ttgatcttag cttattcatc
     3001 gattctgcat gtgttcaatg tacgcagcag ccaatcgatc ttcaaagtca atttagcaac
     3061 caataaagct ttggtagaaa tggcgttact ggcattagct attacaacgg ctgttgcttt
     3121 gctgccattt acgcaagaac tattcggcct agtacacatc agtttgaatc actggttctt
     3181 ggtagggatt ttatctatcg taccaatcgc ggtgaatgaa ctgatcaagt tccatcgatt
     3241 accggaagca gaagaagagg aatgattaac tgtttctaag ttgattttct ttttactgga
     3301 actatacaaa aaggaggtgt gacatgaatg ttgtcacacc tcctttttgc taatgggctg
     3361 aatgtttctt ttactgcctc tcgataaacg gtgttctcct accaacccct cggtcacaag
     3421 tcacactctc ttttaaatat cttttggcaa ttgttcataa aatgctgctt ttgttgcgga
     3481 aatgtatggg ctgagccata gataaccaat accagcagtt gctaatgcta agatatgcca
     3541 gccgataaat gaaagatcta accagaaaag ttgtccttta taaccatcca tcaattttcg
     3601 acttgcagtg atcgtatcca gtactttagg tgtttgtcct gtttcttgga cgatatcgta
     3661 gtagataaaa tagctttgag aataagaata gcttttaatg attccaggaa taactaatag
     3721 caaaccccat aatgttgtaa agatcgagat cagcacagct aagccgatca ctccgccgat
     3781 aaacaaacct ttgaatccac gaaatgcatc tttgaatggt tcgatctgat ctttttgtcc
     3841 acgtaaaata tcaagataag tccaagaaat accgctcatg aacaaagcgc taatgatgct
     3901 ggaaataatt ccgccaccgc ctcctgaact accgcctcct gaagttgtcg catctgagac
     3961 gctcgctgat ccatcatata tggtggtggc aaataagata ataggaatca taataagaag
     4021 aatagcagca ataccaatca atgtagggat tagatttagt aaaacagctt gtccccaacg
     4081 tccttttaga ctctcttttg cttggcgttt taattctgca gaagattgca tgatttttcc
     4141 ctccttttct ttaataacac caatatacca tgtatcaaaa aatatttctg tcataatgtt
     4201 tttctctttt ttgttat
//
LOCUS       DAFBYI010000161         4201 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFBYI010000161 DAFBYI010000000
VERSION     DAFBYI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.166"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(3..1223)
                     /locus_tag="LLD04_002603"
     CDS             complement(3..1223)
                     /locus_tag="LLD04_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK5649492.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1854)
                     /locus_tag="LLD04_002604"
     CDS             complement(1243..1854)
                     /locus_tag="LLD04_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5649493.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="LLD04_002605"
     CDS             complement(1897..2829)
                     /locus_tag="LLD04_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK5649494.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="LLD04_002606"
     CDS             complement(3254..4111)
                     /locus_tag="LLD04_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5649495.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1192 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
     4201 a
//
LOCUS       DAFBYI010000162         4058 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFBYI010000162 DAFBYI010000000
VERSION     DAFBYI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.167"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..1281)
                     /locus_tag="LLD04_002607"
     CDS             complement(<1..1281)
                     /locus_tag="LLD04_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBK5649496.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMN"
     gene            1427..2002
                     /locus_tag="LLD04_002608"
     CDS             1427..2002
                     /locus_tag="LLD04_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649497.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            2216..2911
                     /gene="vanR"
                     /locus_tag="LLD04_002609"
     CDS             2216..2911
                     /gene="vanR"
                     /locus_tag="LLD04_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5649498.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            2889..4043
                     /locus_tag="LLD04_002610"
     CDS             2889..4043
                     /locus_tag="LLD04_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5649499.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1312 a          741 c          806 g         1199 t
ORIGIN      
        1 gttcattcct cggataatct caacagcttg taaaagtggc tcatttgcct ttgtagaatg
       61 aaattccaat actcttaata gcgttggcgt atattttctt agtgaataaa accgtttttg
      121 cagtaagtct aaataatcat agtcggcagg acgtgcaagt tcctgagcct cttctactga
      181 agagacaaag gtattccatt caataaccga ttctaaaacc ttaaaaacgt ctaatttttc
      241 ctctcttgct ttaattaatg cttgtccgat gttcgtaaag tgtataactt tctcatttag
      301 ctttttaccg ttttgtttct ggatttcctc ttgagcctta cgaccttttg ataacaaact
      361 aagtatttgc ctatcatgaa tttcaaacgc tttatccgtt agctcctgag taagttgtaa
      421 taaatagatg gttaatatcg aataacgttt attttcttga aagtcacgga atgcatacgg
      481 ctcgtatctt gagcctaagc gagacagctg caacaggcgg ttacggtgca aatgactaat
      541 ttgcactgtt tctaaatcca ttcctcgtat gtattcgagt cgttctatta tttttagaaa
      601 agtttcgggt gaaggatgac ccggtggctc ttttaaccaa cccaatatcg ttttattgga
      661 ttcggatgga tgctgcgagg taataatccc ttcaagcttt tctttttgct catttgttag
      721 agatttacta accgtattaa atagcttctt ttcagccatt gcccttgctt cccacaccat
      781 tctttcaagt gtagtgatag caggcagtat aattttgttt tttcttagaa aatctatgca
      841 ttcatgcagt agatgaatgg catcaccatt ttccaaagct aattgatgaa ggtacttaaa
      901 tgtcattcga tattcactca gggtaaaagt tacaaagtcg tattcacttc gaatttcttt
      961 caaatgatcc caaagtgtat tttccctttg aggataatga tcaagcgagg atggactaac
     1021 accaatctgt ttcgatatat attgtatgac cgaatctggg atgcttttga tatgagtgta
     1081 tggccaaccg ggataccgaa gaacagctaa ttgaacagca aatcctaaac ggttttcttc
     1141 cctccttcgc ttattaacta tttctaaatc ccgtttggaa aaagtgaagt aggtccccag
     1201 tatccattca tcttcaggga tttgcataaa agcctgtctc tgttccggtg taagcaattc
     1261 tctacctctc gcaattttca ttcagtatca ttccatttct gtattttcaa tttattagtt
     1321 caattatata tcaatagagt gtactctatt gatacaaatg tagtagactg ataaaatcat
     1381 agttaagagc gtctcataag acttgtctca aaaatgaggt gatattttgc ggaaaatcgg
     1441 ttatattcgt gtcagttcga ctaaccagaa tccttcaaga caatttcagc agttgaacga
     1501 gatcggaatg gatattatat atgaagagaa agtttcagga gcaacaaagg atcgcgagca
     1561 acttcaaaaa gtgttagacg atttacagga agatgacatc atttatgtta cagacttaac
     1621 tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac gagataaaaa
     1681 ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata atccatacag
     1741 ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc ttattcggat
     1801 gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag gtcgattaaa
     1861 gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata aagaaggaaa
     1921 tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat tatacaggaa
     1981 attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata tttttaaaga
     2041 agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc cctttgataa
     2101 aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc ttataaattc
     2161 ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga taactatgag
     2221 cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg aattatactt
     2281 aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat tggaatgtat
     2341 agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca caagcggcct
     2401 tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc tgaccgggaa
     2461 agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt atataacgaa
     2521 gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa
     2581 attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa
     2641 tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc ccaccgagtt
     2701 ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg agctgctatt
     2761 tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca ccgtgcatat
     2821 ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata taaaaacggt
     2881 atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa
     2941 aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg ttgtatattc
     3001 gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa aacaaatatg
     3061 acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac aatatagata
     3121 tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc atgctttcaa
     3181 aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt cagaacgaag
     3241 ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc aacacattaa
     3301 aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga aaaaatgacg
     3361 ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc ggttatttga
     3421 gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca
     3481 cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag attacacggt
     3541 ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat atgctggtgc
     3601 agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg gttattcacg
     3661 cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc tttaacaaca
     3721 ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt accgcgggcc
     3781 tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca aaagataagc
     3841 tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc gatacgggtg
     3901 gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga gggcagattt
     3961 acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg atgccagact
     4021 tggttgataa aaggaggtcc taagagatgt atataatt
//
LOCUS       DAFBYI010000163         4056 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFBYI010000163 DAFBYI010000000
VERSION     DAFBYI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.168"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..525
                     /locus_tag="LLD04_002611"
     CDS             <1..525
                     /locus_tag="LLD04_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5649500.1"
                     /translation="PESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQ
                     PKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRD
                     IGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKV
                     GSVQWFAPDFAVSM"
     gene            complement(593..2392)
                     /locus_tag="LLD04_002612"
     CDS             complement(593..2392)
                     /locus_tag="LLD04_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK5649501.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2515..3021
                     /locus_tag="LLD04_002613"
     CDS             2515..3021
                     /locus_tag="LLD04_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5649502.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3100..3822
                     /locus_tag="LLD04_002614"
     CDS             3100..3822
                     /locus_tag="LLD04_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5649503.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3900..>4056)
                     /locus_tag="LLD04_002615"
     CDS             complement(3900..>4056)
                     /locus_tag="LLD04_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK5649504.1"
                     /translation="TGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVMKELFGMVEK
                     GTPVLVF"
BASE COUNT         1203 a          770 c          855 g         1228 t
ORIGIN      
        1 cctgaatctt caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat
       61 caaagtgttg atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca
      121 acacctgaac aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt
      181 gcagcaatcg tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa
      241 gatccaagtg gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt
      301 cgtgacatcg gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct
      361 caagcaaaag ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg
      421 attgctttag gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt
      481 ggttcagttc aatggtttgc ccctgacttt gctgtcagca tgtaagaatg attaatcaaa
      541 aagagttagc ccattctgga ctaactcttt ttttattgtg ataaaataga aatcagataa
      601 attcttctgt caattccatg aacgtttgga tatcttcagt caccatgtcg atacctgctt
      661 gccaaaaatc tggttgggta agatcgacat tcaaatgttt cttcgcaaga tcttccgtcg
      721 tcattgaggc agtatcgcgt agtaaatgga tatattcttc ttcaaaattt gctccctttt
      781 ggttggcgta agcatagatg cccaaactaa agaggtagcc aaatgtgtat gggaagttat
      841 aaaacggtac gtcatcaata aagaaatgca attttgcagc ccagaaatat ggatggtaag
      901 tagctaaagc tccttgatag ccttcttttt gcgcctcaac catcatctct gtgatcttat
      961 cttctgagac taatccttct tgccgtgctt catagaatcg attttcaaaa atgaatcggg
     1021 aatggatatt catgaacata gctaaagcat tttgcagttt tgtatctagc aaattgattt
     1081 tttcttcttt tgtttttgcg acttttaatg tagcatcggc tacgatcagt tctgcgaatg
     1141 tacttgctgt ttctgcgaca ttcatggcat attcgcgatt taaagaaggt aaatcccaca
     1201 tcgtgctgct atggaaagca tgtcctagtt catgagcaag tgtcgcaacc tcattaacag
     1261 aattactgta ggtcataaag atccttgatt cttttgtttc tggtaactca gtacaataac
     1321 caccaggacg tttcccagga cgatcttctg cttcgatcca gctcttttca aaagcagatt
     1381 gggcaaattt agccattttt ggactgaatt tgttaaagtt ttcgataata aatgctgctg
     1441 cttcgtcaaa actaaaggtt ttttctttca aatcgcctaa aataataggt gcatcttgat
     1501 cttgccattc cattttttct ttaccgaaaa gattagcttt tctagtcaag taattaacaa
     1561 ggggttgttt gtttttctgg attgtatccc acatgacaga caatgtttct ttcttcaacc
     1621 gattgtactc taatggtttt tgtagaaaat ctgtgacacc atgaagttca tagtcagaaa
     1681 gtctaaaacc atctaaatga ttcaaagtat ccgtgaagag gggtgccttt tctttccagg
     1741 cttcttccca tgctgcgaaa agtgtttctc tgacttttgg atctggatcg cccatcatct
     1801 tattgaaggc ttgtccagca gataattcga ctacttgtcc atcttgttcg aagggaatcg
     1861 aaatgctggc tacaatcgta tcataatgac tgctccaagc atttagacca tctaaagaaa
     1921 gcgtatttat aatgttttct tcagcttctg ataataattg tgagccatca cgacgaatct
     1981 cgtttaaacg aaaagcaatt gtttcaaacg aagattgaga aagcatctga ttccaatcat
     2041 tgtcagatat ttctgcaaat ttctttgaca atacagtttc ggctgattgc agacgaggca
     2101 gcattgcgta aagcttgcct gataagacct ttgcatctga atcattgaca ttggcagaaa
     2161 gcaatgcagt aatatagctg ctgcattggg taaaaccatt cgtaatcgtt tcttgcaatt
     2221 gaagaatagc gtttaactgc tctgcattgt cagaactagg tgaccatttt gttacgcggt
     2281 gataatactc gttcatttgg tcttccaatt gcttcatccg ttggttcaaa gcatcagaat
     2341 gactgcctcc ggaaaaaata gaatctagat tccaatttaa tgaataactc atgaaaaaac
     2401 tcccttcata ttgtaataca aaattaagta tagcactttt ttatggaaga agtctctatg
     2461 aaaatgattg ttgtttcttc aaaaaaagtc taagataaaa tacagaggtg acttatgaat
     2521 tcttcaaaaa aacaaatgaa aaacggaaat atttatttag taatcgcact gatagtcgtt
     2581 ataatcttat tttacagttc ttctcaaact tacggacagc aatcacaagt ttcgcgatta
     2641 gaaagctggc tgcctaatca gccatttaaa gacacattag cgggtatacg ttttaattat
     2701 ggtggaagta tggtgagtat cgaccatctg ggctacgcca aatttttaga gttcttttta
     2761 aggaagggcg cacatttcgg tacctatttt attttgggag gcagcttgtt tttaggtgtt
     2821 ttccccaagc taaaaatttg gtggctgaca gcgatccttg cttggctttc agcaacaggg
     2881 tacgcaggtt tggacgaatt ccatcagatg atgaccggtg gacgaacacc tatgttccaa
     2941 gatgtcatgt tggattcgat cggtgctctg actgctgtga tattatgtgt gctttttgcg
     3001 gcattcaaga agcgtttata gattggctgt ggctttgttt ttttgtgaaa tagtactaga
     3061 atagaagtgt attctcaaaa gaacaaggga ggaaaaatca tgtcaggaca tagtaaatgg
     3121 cataatatcc aaggaagaaa aaatgcccaa gatgcaaaac gagggaaagt ctttcagaaa
     3181 ctttctcgtg aaatttatat ggcagctaaa gcaggcggtc ctgatccatc tatgaatcca
     3241 gctttgcggc ttgttgtgga caaagcaaaa gctgcaaata tgcctaacga caatgtggaa
     3301 cgggcgatta aaaaggctac gagtgccgta gatgaaacga attacgatga aatcacgtat
     3361 gaaggatatg gtccgggtgg agtaggaatc ttagtccatg cattgacaga taaccgtaat
     3421 cggacagcga caaatgtccg agtagctttt actcgaaacg gtggatcact gggagaaacg
     3481 ggttcagtca gttacttgtt tgaccgcaaa ggatatatcg ctattgaacg cgatgggttg
     3541 actgttgatg aagagacgat gtttgaggat gtattagagg cgggggcaga agatctgcaa
     3601 acttctcctg aagtattcga gatttatact gcaccagaag attttgctgc tgtacgagat
     3661 gagttggaaa aagagttcac tttggcgcaa gcagaattga cgatgattcc acaaacgacg
     3721 attgatttaa atgaggaaca aaaagagcaa ttggaacgtc tagtagataa gctagaagac
     3781 gatgatgatg tttcagaagt ctttactgct gctgaaatgt aagtagcaca ttgttagata
     3841 ataaaaagaa agcacctgta ccaatcgtac gggtgctttc tttttatata ttatttttgt
     3901 tagaagacca atacaggcgt tcctttttcg accatcccga ataattcctt cattacactt
     3961 ggtggggtgt tgatgcagcc gtgtgaaccg cgggttttcc ataagtctcc accgtattca
     4021 ggttgccagt ctgaatcgtg gataccaaca cctgtc
//
LOCUS       DAFBYI010000164         4035 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFBYI010000164 DAFBYI010000000
VERSION     DAFBYI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.169"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            178..774
                     /locus_tag="LLD04_002616"
     CDS             178..774
                     /locus_tag="LLD04_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5649505.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="LLD04_002617"
     CDS             898..2697
                     /locus_tag="LLD04_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5649506.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="LLD04_002618"
     CDS             complement(2975..3187)
                     /locus_tag="LLD04_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649507.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="LLD04_002619"
     CDS             3649..3798
                     /locus_tag="LLD04_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649508.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1185 a          688 c          712 g         1450 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat attta
//
LOCUS       DAFBYI010000165         3995 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFBYI010000165 DAFBYI010000000
VERSION     DAFBYI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.170"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            65..739
                     /locus_tag="LLD04_002620"
     CDS             65..739
                     /locus_tag="LLD04_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5649509.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            732..896
                     /locus_tag="LLD04_002621"
     CDS             732..896
                     /locus_tag="LLD04_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649510.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            972..2114
                     /gene="dcm"
                     /locus_tag="LLD04_002622"
                     /pseudo
     CDS             972..2114
                     /gene="dcm"
                     /locus_tag="LLD04_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2104..2394
                     /locus_tag="LLD04_002623"
     CDS             2104..2394
                     /locus_tag="LLD04_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649511.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2653..3837
                     /locus_tag="LLD04_002624"
     CDS             2653..3837
                     /locus_tag="LLD04_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5649512.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            3834..3971
                     /locus_tag="LLD04_002625"
     CDS             3834..3971
                     /locus_tag="LLD04_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK5649513.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1297 a          664 c          922 g         1112 t
ORIGIN      
        1 ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa aggggataat
       61 taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat
      121 ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg
      181 gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca
      241 tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaactc
      301 tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat atgtcttaga
      361 tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat
      421 ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc aagaccctaa
      481 aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa
      541 ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca
      601 tccagatgta ttgcttcata tcataataaa agattatacg aataatattt ccgaagttca
      661 ttatgctacg gataatctag aattattttt accactaggg tatagtagga aagatttgga
      721 ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa tacaataaga
      781 gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg attaaatatg
      841 tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa aaatagtagg
      901 acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct ttgcaatgaa
      961 tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt cgtttaggta
     1021 tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt gctagacgta
     1081 gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc acaagtgtat
     1141 cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga tttccgtgcc
     1201 aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt actttattct
     1261 ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt gagaacgtcc
     1321 gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga acgctggatg
     1381 gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt ccccaaagcc
     1441 gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa gtatttccta
     1501 tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat atcaatcctt
     1561 ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca accttaacag
     1621 gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta gcaggactat
     1681 taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc gcccccacct
     1741 tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca gaatatcaag
     1801 aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt cgccgaggtc
     1861 gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg atcgtcacgg
     1921 atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag ggctttcccg
     1981 attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat aagcaagccg
     2041 gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa gagttggatg
     2101 tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa gattcagtca
     2161 gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa gaatggtgtc
     2221 aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat tgaaataaat
     2281 caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga tattcgtcaa
     2341 tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga gtaacctaaa
     2401 ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga aatcctccgt
     2461 ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc ccgtatctaa
     2521 caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct tgttgtatca
     2581 aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca ccttgaattt
     2641 ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga ccgacgcaac
     2701 gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg gccgtactta
     2761 aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa aatttttgac
     2821 caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta tgtaaggatt
     2881 cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt gaaatttgag
     2941 tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt catgggtgat
     3001 attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt aaaggggcga
     3061 ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta cgactttttc
     3121 gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat caatgatcga
     3181 gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga atgtatctcc
     3241 ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga tgaaaaggac
     3301 ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt ctgtttgtat
     3361 gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac tgagacgaaa
     3421 aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca agatttgtta
     3481 aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt gcgatttgcg
     3541 gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg ggggcgtttt
     3601 attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta taccattgaa
     3661 cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc taaggagttg
     3721 gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg cttatcagac
     3781 cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat tatatgaaaa
     3841 gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc agtatgcttg
     3901 gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca atggaaaaga
     3961 aaggaaagtg acaagatgaa tttcggacaa aattt
//
LOCUS       DAFBYI010000166         3956 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFBYI010000166 DAFBYI010000000
VERSION     DAFBYI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.171"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..156
                     /locus_tag="LLD04_002626"
     CDS             <1..156
                     /locus_tag="LLD04_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK5649514.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="LLD04_002627"
     CDS             175..1452
                     /locus_tag="LLD04_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5649515.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="LLD04_002628"
     CDS             1462..2118
                     /locus_tag="LLD04_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5649516.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="LLD04_002629"
     CDS             2118..3494
                     /locus_tag="LLD04_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5649517.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="LLD04_002630"
     CDS             3624..>3956
                     /locus_tag="LLD04_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5649518.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFBYI010000167         3883 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFBYI010000167 DAFBYI010000000
VERSION     DAFBYI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.172"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            91..3123
                     /locus_tag="LLD04_002631"
     CDS             91..3123
                     /locus_tag="LLD04_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK5649519.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="LLD04_002632"
     CDS             3313..>3883
                     /locus_tag="LLD04_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBK5649520.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DAFBYI010000168         3810 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFBYI010000168 DAFBYI010000000
VERSION     DAFBYI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.173"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            94..2193
                     /locus_tag="LLD04_002633"
     CDS             94..2193
                     /locus_tag="LLD04_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5649521.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="LLD04_002634"
     CDS             2389..3252
                     /locus_tag="LLD04_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK5649522.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3810
                     /locus_tag="LLD04_002635"
     CDS             3357..>3810
                     /locus_tag="LLD04_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5649523.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1524 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa
//
LOCUS       DAFBYI010000169         3800 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFBYI010000169 DAFBYI010000000
VERSION     DAFBYI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.174"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(23..637)
                     /locus_tag="LLD04_002636"
     CDS             complement(23..637)
                     /locus_tag="LLD04_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649524.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="LLD04_002637"
     CDS             951..1373
                     /locus_tag="LLD04_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649525.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="LLD04_002638"
     CDS             1383..1586
                     /locus_tag="LLD04_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649526.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="LLD04_002639"
     CDS             1797..2381
                     /locus_tag="LLD04_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649527.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>3800
                     /locus_tag="LLD04_002640"
     CDS             2550..>3800
                     /locus_tag="LLD04_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5649528.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1282 a          602 c          717 g         1199 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgagaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact ggttcaggat
     2701 tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat gtgagtagac
     2761 cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca cgtatgaacc
     2821 tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag
     2881 atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt
     2941 ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac
     3001 gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg
     3061 ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt
     3121 acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg
     3181 gttcgcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta
     3241 actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc
     3301 atgctaatgg gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat
     3361 catatggaaa ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg
     3421 tggtaaaaga aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag
     3481 gttgtgtcca tttacacatt ggcacgtcga tcttagaaac gagaccaggg ttttcacacc
     3541 agatgaagat ccctatcaca gataatacaa aggaattgca gaattattgt cttttcttgt
     3601 ttgataagta ttatgaagga caagaagtaa ggcatgttgg aataacctat agcaagcttg
     3661 tgtacaccga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag
     3721 aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac
     3781 atgcttcttc aatgttagaa
//
LOCUS       DAFBYI010000170         3762 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFBYI010000170 DAFBYI010000000
VERSION     DAFBYI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.175"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(53..2545)
                     /locus_tag="LLD04_002641"
     CDS             complement(53..2545)
                     /locus_tag="LLD04_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK5649529.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="LLD04_002642"
     CDS             complement(2552..3019)
                     /locus_tag="LLD04_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK5649530.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3155..3228)
                     /locus_tag="LLD04_002643"
     tRNA            complement(3155..3228)
                     /locus_tag="LLD04_002643"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Arg,seq:acg)
     gene            complement(3239..3324)
                     /locus_tag="LLD04_002644"
     tRNA            complement(3239..3324)
                     /locus_tag="LLD04_002644"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3288..3290),aa:Leu,seq:taa)
     gene            complement(3341..3412)
                     /locus_tag="LLD04_002645"
     tRNA            complement(3341..3412)
                     /locus_tag="LLD04_002645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3378..3380),aa:Gly,seq:gcc)
     gene            complement(3424..3496)
                     /locus_tag="LLD04_002646"
     tRNA            complement(3424..3496)
                     /locus_tag="LLD04_002646"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3461..3463),aa:Thr,seq:tgt)
     gene            complement(3514..3595)
                     /locus_tag="LLD04_002647"
     tRNA            complement(3514..3595)
                     /locus_tag="LLD04_002647"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3559..3561),aa:Leu,seq:tag)
     gene            complement(3603..3675)
                     /locus_tag="LLD04_002648"
     tRNA            complement(3603..3675)
                     /locus_tag="LLD04_002648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3640..3642),aa:Lys,seq:ttt)
     gene            complement(3679..3751)
                     /locus_tag="LLD04_002649"
     tRNA            complement(3679..3751)
                     /locus_tag="LLD04_002649"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3716..3718),aa:Val,seq:tac)
BASE COUNT          991 a          869 c          663 g         1239 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt cgaaccccta
     3181 accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat atttgaaatg
     3241 ccgaggaccg gaatcgaacc ggtacggtga tcactcaccg caggatttta agtcctgtgc
     3301 gtctgccagt tccgccaccc cggcgcgatt gctttggcaa agcggaaaac ggggttcgaa
     3361 cccgcgaccc ccaccttggc aaggtggtgc tctaccactg agctatttcc gcgtgtttaa
     3421 agatgccggc taaaggactt gaaccctcga ccctctgatt acaaatcaga tgctctacca
     3481 actgagctaa gccggcatgt aaaacttaaa ttatgcgggt gaagggactt gaacccccac
     3541 gccgtaaggc gctagatcct aaatctagtg cgtctgccaa ttccgccaca cccgcaaaaa
     3601 tatgagccgt acagggctcg aacctgtgac cctctgatta aaagtcagat gctctaccaa
     3661 ctgagctaac ggctcatatg gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag
     3721 gcagatgctc tcccagctga gctaaacctc catggataac tt
//
LOCUS       DAFBYI010000171         3625 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFBYI010000171 DAFBYI010000000
VERSION     DAFBYI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.176"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(62..205)
                     /locus_tag="LLD04_002650"
                     /pseudo
     CDS             complement(62..205)
                     /locus_tag="LLD04_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            287..1063
                     /locus_tag="LLD04_002651"
     CDS             287..1063
                     /locus_tag="LLD04_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5649531.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(1161..1808)
                     /locus_tag="LLD04_002652"
     CDS             complement(1161..1808)
                     /locus_tag="LLD04_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5649532.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(1808..2584)
                     /locus_tag="LLD04_002653"
     CDS             complement(1808..2584)
                     /locus_tag="LLD04_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5649533.1"
                     /translation="MCLRSWTIMCSWNYERQMHMGFMYGMAPILNKVYKNDEEKKREA
                     YTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTESISMIKTSLMGPFAGIGD
                     SFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSILAVFYGGKLGYESGNKYLT
                     KLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFTTGGTELVLQDLLDSIIPG
                     LLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL"
     gene            complement(2637..3386)
                     /locus_tag="LLD04_002654"
     CDS             complement(2637..3386)
                     /locus_tag="LLD04_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5649534.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            complement(3400..>3625)
                     /locus_tag="LLD04_002655"
     CDS             complement(3400..>3625)
                     /locus_tag="LLD04_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649535.1"
                     /translation="KGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAVFLTPEEL
                     EATKKILDQEVKIFVQQVPSSSIENVDFSK"
BASE COUNT         1278 a          635 c          504 g         1208 t
ORIGIN      
        1 aaaaaaacaa acaaagtaaa ataatctact ttgtttgttt ttattttaat taatattgaa
       61 tttataaaat tgtcttatca cttggttcca caaccaagta gcgataaggc tcatcccctt
      121 cagaatgagt ggtaacataa ggatttttac ttaaaacaga atgaatttgt tttctttcaa
      181 aagcgggcat tggttagtca aagactagtt catttatatt tatatcttaa tcttttattt
      241 taataaattg tgatataatg aagtataact ttttagggag tgtactatga attttcaaga
      301 taatagacca ttatatctac aagtagaagc ttctatacgt aacgatattc gtgaaaaaaa
      361 atatcttcca ggagagcaat tacctacaga agaagaatta tgtaaaattt atggagtaag
      421 taagattacc attcgaaaag cttttaaact tttaacagaa agtggaacag ttgaacgatt
      481 aagggggaaa gggacctttg taactcaaaa aaaagaaagt ttaaaattag ctggagttca
      541 tggatttagc gattctcttt ctacccgagg acataaaatt agatatactg ttttacattc
      601 taaaattata aaggctgatg agtttttagc taataaatta caaatcaatg taactgatag
      661 agtttacaat attaaacgtt taatgtggga agacggtgcc cctatgggaa ttgataattt
      721 tttcgcatca gaagaaaaat ttccaacttt atttgaaaaa ggtagccaat atacttcatt
      781 atataaactt ctagaagatg actatgatgt caaagctacg agctctatta tggaaataaa
      841 cggtttatct gcaacacctg agctttccga cttattgcaa tgtatagtag gagatccgct
      901 atttatttta gaaaaggttg gcaaagataa aaataatcaa ccatttcatt attctcaatc
      961 cacagttcgt tgtgatagaa ttagttatgt tattgaagtc acagaaaata tcactttaga
     1021 tacggtgcat aaaaatgatt cagatcctaa agtaatagat tgatattaaa agaaatcata
     1081 cccaaagcaa tcaattttga ttagatttgg gcatgatttt tatttttact aatattctct
     1141 aaatacatca aaaaacttat ttatattcca aatttcacct gtacttcttg tcgcagatat
     1201 ttaatatcgt aaataacatc atctgctaga cttacatctt gagaaaaacg tttatcttta
     1261 attgcaagaa ccttcatatt tgctctttta ccagctttta taccattata agaatcttct
     1321 atcaccagac attcttcaac tggaattctg agctgttctg aagctttaag gaatatagta
     1381 ggattaggtt tactttctgg caaatctctt ccgctaatta aacaatcaaa atatgactct
     1441 attttacaag tggtaacaac ttcatttata gtatccattg gactggaaga agcaattgcc
     1501 atttttattt ttttcttttt taaccattca agctgttcaa ttagtatatt aggaaaaata
     1561 tccaagtaat taatcttttg cgatttccaa tatttagtaa attttccctt caattctaaa
     1621 ctgttacatc cctcattgta cttttctata tcatcaaaaa tacttgctgt agttccaatc
     1681 tttttttgat aagaatacaa aggatagctt aaattttctt gttttaaaaa attaaatacc
     1741 cacttttgat acataatctc gctatcaatt aaaactccgt ccatatcaaa aattagagcc
     1801 ttaatcatta taagatacct attatactta aaacaaaccc tgccaaaata caacctaata
     1861 agagcatatt tgtactaaca tttttcttaa tcaatttata tattcccaaa gtacatacaa
     1921 gtggtaataa tccaggaata atggaatcaa gtaagtcttg taaaacaagt tctgttcccc
     1981 ctgtagtgaa ttttaaaggg gtgacaattg gcaccatgct agccaccatt ccacctacta
     2041 cagccaaacc aatgatagaa gcaaattgca ttactcgatc cataagacct tcttgataca
     2101 atttagtaag gtatttattg ccagactcat atcctaattt accgccgtaa aaaacagcta
     2161 aaatagaagg tatagcaaaa aggccaacag ctaaaatcgg tcctaaaata ctaccttgct
     2221 tagcaaaaga caatccaatc ccaaatgtaa taattcttaa agtaccttgg aaaaatgaat
     2281 caccaattcc agcaaaagga cccattaaac tggttttaat catggagata gattcagtat
     2341 taaagtttcc ttcttctgct tgagcatttt gttcttccat agaagcacaa agtcccataa
     2401 taaatggagt catttgaggc gtacagttaa aaaatgccat atgacgagta taagcttctc
     2461 tttttttctc ttcatcattt ttataaactt tatttaaaat tggcgccatc ccatacataa
     2521 atcccatatg catttgtctt tcataattcc acgaacacat gattgtccat gaacgaagac
     2581 acatcttatg aagatctttt tttgtgatta aacgtttttt tctgtactat tttccattaa
     2641 ccatgcgcct cttctctttt tccatattta acttgatcca ttacaaaggc aatcagagct
     2701 gcaaaaattg tcacgccaat tagtgaaaga cctgaatagg caacaataaa gaatccaaaa
     2761 ataaagtaag gatataattt tttactcaac attgtgctta atagcattgc aaaccctaat
     2821 gcggaaagca tatttccaga gacagtcaag gcgtctgtaa tcatttctgg aattttatct
     2881 actaaatctg ccacaacgtc agcaccaaaa tatactgtta aaaagattgg aataaagtaa
     2941 acaaaagcat taaaaataaa tgaccaataa atatgaataa tttgagcttt tctaaattta
     3001 ttattttcaa tggctttatc tgcagcatgt ccaaattgag caattatcat gcgcatgata
     3061 atactcataa attcaccagc tacagccact gggatagcta ttgttaaggc tgtttctgta
     3121 ctactattag ttaaaattgc aaaagcagtg gcaataatac taccaagttg catattagga
     3181 ggtccagccg ccccaattga gacgaatccc atagaaatta attctaaaga tgccccaaga
     3241 acaatccctg tatgcaagtc acctagcaca agtccggcaa gtgtactcaa aattagtggt
     3301 ctaccaatat tttgtcgacc aattaacctg gagtcaataa cagctaccac accaactaaa
     3361 cctaataaaa tggcttttaa taacatttat tttcccccct tacttactaa aatctacatt
     3421 ttcaatactt gaagatggga cttgttgcac aaatattttc acttcttgat caagaatttt
     3481 ttttgtggct tctaattctt caggagttaa aaagactgcc tttccatatt gtttggactc
     3541 gcttttctta gacacaccgc caaaattaat ttctttaatt tctggtaaat tttgaactaa
     3601 ttcaaaagct tccttaggtc cttta
//
LOCUS       DAFBYI010000172         3589 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFBYI010000172 DAFBYI010000000
VERSION     DAFBYI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.177"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            340..1380
                     /locus_tag="LLD04_002656"
     CDS             340..1380
                     /locus_tag="LLD04_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5649536.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2204..2536
                     /locus_tag="LLD04_002657"
     CDS             2204..2536
                     /locus_tag="LLD04_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK5649537.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3155..3496
                     /locus_tag="LLD04_002658"
     CDS             3155..3496
                     /locus_tag="LLD04_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK5649538.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1325 a          473 c          669 g         1122 t
ORIGIN      
        1 gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt tcttaattta
       61 ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta aattttattc
      121 atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag ttcttaatga
      181 ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa
      241 cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat
      301 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
      361 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      421 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      481 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      541 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      601 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      661 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      721 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      781 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      841 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      901 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      961 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
     1021 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
     1081 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt
     1141 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
     1201 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
     1261 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1321 gaaaaacgtt tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga
     1381 aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta
     1441 ttattctaat tatattacga atattacaaa ataatagcta ttttaataga atttataagt
     1501 taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat
     1561 aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat
     1621 aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact
     1681 tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc
     1741 tatgcttgtg atgaattgat tttcgttctg cacttttgga tgataaattt ggacaattga
     1801 aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
     1861 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
     1921 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
     1981 gttaaaaata cgtgatatta cgatgaatct atgcctaagc acaatgttat aaaaatgtcc
     2041 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
     2101 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     2161 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
     2221 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
     2281 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
     2341 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
     2401 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
     2461 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     2521 gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     2581 tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac
     2641 ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt
     2701 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     2761 aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc
     2821 tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc atggaataag
     2881 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     2941 tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta
     3001 gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac
     3061 gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata taaaacatag
     3121 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     3181 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac
     3241 gtttagaaga ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt
     3301 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     3361 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     3421 agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc
     3481 ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc
     3541 acgatgatta ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DAFBYI010000173         3543 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFBYI010000173 DAFBYI010000000
VERSION     DAFBYI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.178"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(560..1600)
                     /locus_tag="LLD04_002659"
     CDS             complement(560..1600)
                     /locus_tag="LLD04_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5649539.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="LLD04_002660"
     CDS             complement(1949..2275)
                     /locus_tag="LLD04_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649540.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="LLD04_002661"
     CDS             complement(2262..3065)
                     /locus_tag="LLD04_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5649541.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1084 a          584 c          487 g         1388 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aac
//
LOCUS       DAFBYI010000174         3401 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFBYI010000174 DAFBYI010000000
VERSION     DAFBYI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.180"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(74..1072)
                     /locus_tag="LLD04_002662"
     CDS             complement(74..1072)
                     /locus_tag="LLD04_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5649542.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="LLD04_002663"
     CDS             complement(1147..1443)
                     /locus_tag="LLD04_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5649543.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="LLD04_002664"
     CDS             complement(1436..1729)
                     /locus_tag="LLD04_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649544.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1875..2426)
                     /locus_tag="LLD04_002665"
     CDS             complement(1875..2426)
                     /locus_tag="LLD04_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBK5649545.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2600..2899)
                     /locus_tag="LLD04_002666"
     CDS             complement(2600..2899)
                     /locus_tag="LLD04_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBK5649546.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2926..3192)
                     /locus_tag="LLD04_002667"
     CDS             complement(2926..3192)
                     /locus_tag="LLD04_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5649547.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          993 a          668 c          461 g         1279 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacacacccc
     1741 ctgttatttt ttacataatg aaaatagcat attactgggt acattgataa ttcatataac
     1801 atgtatcatc acatgtattt ttaaagtcaa aaagcatgta aaactactta taagtaacat
     1861 gagaaaataa aatgttattt ttcttgaaat aataattgac tatctgccaa ttgccattca
     1921 ttacgaacat gaatacttag tgggtgggat aacttacctt ctgcatttat caatactttg
     1981 gtcatcttct tttgtttcgc actcaattga tcgtaaatag tctttgcttg ttcataagcc
     2041 acttgactcg tcgctgtaat cgaaccacaa gcaaaaataa tggtattgat tttcttttct
     2101 gtaaaaacag tcgttaatat ttccaaagat tgttcatcat ttcctcgaag aaatacccga
     2161 ggacttttcg cttttgtgta cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat
     2221 tgtcggatat tcttttcgat taataacata ctcaaatcat tcgtaaatgg ttctgtatct
     2281 gttggacgaa tagtaatgac tactgctaat ttatcttccg cttcttttcc tttgatcgcc
     2341 cattgtcgtt tgaaaacata gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt
     2401 gtggtcacta attctttttc aatcatctct ttttctccta tgctattttt tgttcaagca
     2461 cttcaattac cattgccgtt ataagtgtca atactagttg aaaatatttc atattctttc
     2521 tccttttttc ttcagaattt gatgaatcgc ggtaaggata tcttctaaac atttcgcaat
     2581 cattctcaaa taatcgatct tattcatccg ttcctccaat ctgactccaa accaatcgtc
     2641 cttctaccag taacccacga acaaattctc gctggctggt aatatctgta atgaaataaa
     2701 ctacatagtc ttttccatct tctggtgcgc aataaatata aagatgtgtc atcactaaac
     2761 gtggtagatg aacatcttca ggaagcatac ccatttcaat ttcctctttt tccatcgtaa
     2821 cggattcata gccccactct tcagaagcag tgaccagctg tgcacacttt tccagtactt
     2881 cgatagccgt ttctaacaag tcctttcgtt cctttctttt agatactagt attcttctgg
     2941 taataacatc gtttgatact cacccgaatc aatggcccat attttttgag acactgattt
     3001 caaacgaagc ttaggtgaaa tcagatactc ttttctttct tccggttctt cttgacgatg
     3061 aaccaacttt tgatttcctt tttccgtaga aaattcaaaa atttgaagat agtctaaagg
     3121 aaattgcttt tgaacgtttt gatcgattag attccaacat aaagcttgta attccaccgg
     3181 caaactgtcc attactccct ttgtgacata acgttatcct ttattttgaa acatttcttt
     3241 tccctccttt gccataaaaa tagcccaatc ttctattaaa gaagattggg cttcatggat
     3301 ataatatata tttgtaaata aagttaattc agttataatt atcaaaacta cacaattatt
     3361 aaagtttttc atatctcttt gtttagtaag tttttctaaa t
//
LOCUS       DAFBYI010000175         3399 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFBYI010000175 DAFBYI010000000
VERSION     DAFBYI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.181"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..277
                     /locus_tag="LLD04_002668"
     CDS             <1..277
                     /locus_tag="LLD04_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBK5649548.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="LLD04_002669"
     CDS             420..641
                     /locus_tag="LLD04_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5649549.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="LLD04_002670"
     CDS             657..1499
                     /locus_tag="LLD04_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649550.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="LLD04_002671"
     CDS             1562..3364
                     /locus_tag="LLD04_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK5649551.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1229 a          469 c          703 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DAFBYI010000176         3387 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFBYI010000176 DAFBYI010000000
VERSION     DAFBYI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.182"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(27..530)
                     /locus_tag="LLD04_002672"
     CDS             complement(27..530)
                     /locus_tag="LLD04_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5649552.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="LLD04_002673"
     CDS             complement(653..1417)
                     /locus_tag="LLD04_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK5649553.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1525..1800
                     /locus_tag="LLD04_002674"
     CDS             1525..1800
                     /locus_tag="LLD04_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK5649554.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2238..3188
                     /gene="yidC"
                     /locus_tag="LLD04_002675"
     CDS             2238..3188
                     /gene="yidC"
                     /locus_tag="LLD04_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5649555.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         1010 a          707 c          644 g         1026 t
ORIGIN      
        1 atatggtcta ttattggttt tatctattaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgg tgacataggc tgagcggctt ctttgactgc gcagtatttg
      121 tctacgaatg ttacgatata tccttctttg tacttaggcg gtgcaatcgt tgcgccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaatt cggtgatctt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttctacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgccttg gataaaatag ccaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacaatc cctgccgcaa ccccaacatt caacgattcc gcatttcctt
      721 tcatcgggat atatacgttc tgatcagtca aagacaagat ttcctgactt accccctgac
      781 cttcgttccc catgattatt gcgaagttat ctgatttttc tactttgtct aatgcgtgtg
      841 cttcttcatt taattctgtg ccatacactg taaacccttt gtctttcatt aaaggaatca
      901 cttctgtcaa atctttttgc aacacaggca aatgataatt gcttccctgc atgctgcgca
      961 gcacctttgt actataaata tctgccgttc ctttgcctaa taaaacacca gccatatcaa
     1021 atgcatctgc cgtacggatc atcgtcccta catttccagg atcttgtaca ttatctaaca
     1081 acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt taacgccatc actgctaaga
     1141 ttccttgtgg tgtaggtaga tccgatacag catccatgac ctcatccgaa actagaaaag
     1201 tagaaatctc tttgtttgcg gccagccagt ctccccattc tttcatgcct cgctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcaatcaga tggaaccctt
     1321 ccaaaagata ttcttccttt tcttcccgat atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctcc ccatgattat
     1441 atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttaatttata
     1501 aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc
     1561 caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg cgtgactgga
     1621 acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa tgacgacatc
     1681 atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg agtgcagtat
     1741 gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt ggtgggataa
     1801 ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa atagctctcc
     1861 atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcgataa
     1921 ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa gctgatctat
     1981 cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt tttagtttat
     2041 taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc tttcttgatt
     2101 ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat atgaaatttc
     2161 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
     2221 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
     2281 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
     2341 cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
     2401 aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc gtccgtatca
     2461 tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
     2521 aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca acaacacgtg
     2581 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac ggattgagta
     2641 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
     2701 actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc atcgatttag
     2761 gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt caaggataca
     2821 tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
     2881 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
     2941 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
     3001 gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa gtcgtcactc
     3061 caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca accaaacaaa
     3121 tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag cagcagaagc
     3181 ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa
     3241 tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag tgaatgttta
     3301 atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac
     3361 tttttcttat ctatcaacgt agacagg
//
LOCUS       DAFBYI010000177         3374 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFBYI010000177 DAFBYI010000000
VERSION     DAFBYI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.183"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            254..580
                     /locus_tag="LLD04_002676"
     CDS             254..580
                     /locus_tag="LLD04_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649556.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="LLD04_002677"
     CDS             complement(697..1041)
                     /locus_tag="LLD04_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649557.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="LLD04_002678"
     CDS             complement(1117..2364)
                     /locus_tag="LLD04_002678"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK5649558.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="LLD04_002679"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="LLD04_002679"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK5649559.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          670 g         1051 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agta
//
LOCUS       DAFBYI010000178         3369 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFBYI010000178 DAFBYI010000000
VERSION     DAFBYI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.184"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LLD04_002680"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LLD04_002680"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK5649560.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="LLD04_002681"
     CDS             complement(2261..2512)
                     /locus_tag="LLD04_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK5649561.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="LLD04_002682"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="LLD04_002682"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK5649562.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          919 a          740 c          626 g         1084 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaa
//
LOCUS       DAFBYI010000179         3360 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFBYI010000179 DAFBYI010000000
VERSION     DAFBYI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.185"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            117..1106
                     /locus_tag="LLD04_002683"
     CDS             117..1106
                     /locus_tag="LLD04_002683"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK5649563.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1423..1947)
                     /locus_tag="LLD04_002684"
     CDS             complement(1423..1947)
                     /locus_tag="LLD04_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK5649564.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2234..>3360
                     /gene="scm"
                     /locus_tag="LLD04_002685"
     CDS             2234..>3360
                     /gene="scm"
                     /locus_tag="LLD04_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK5649565.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSAS"
BASE COUNT         1073 a          685 c          621 g          981 t
ORIGIN      
        1 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
       61 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
      121 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
      181 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
      241 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
      301 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
      361 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
      421 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
      481 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
      541 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
      601 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
      661 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
      721 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
      781 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
      841 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
      901 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
      961 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
     1021 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
     1081 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
     1141 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
     1201 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
     1261 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
     1321 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
     1381 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
     1441 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
     1501 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
     1561 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
     1621 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
     1681 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
     1741 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
     1801 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
     1861 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
     1921 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
     1981 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
     2041 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
     2101 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
     2161 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
     2221 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
     2281 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
     2341 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
     2401 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
     2461 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
     2521 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
     2581 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
     2641 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
     2701 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
     2761 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
     2821 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
     2881 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
     2941 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
     3001 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
     3061 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
     3121 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
     3181 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
     3241 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag attcatcaac
     3301 ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggata catcagcttc
//
LOCUS       DAFBYI010000180         3282 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFBYI010000180 DAFBYI010000000
VERSION     DAFBYI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.186"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            647..964
                     /locus_tag="LLD04_002686"
     CDS             647..964
                     /locus_tag="LLD04_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5649566.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="LLD04_002687"
     CDS             complement(1055..1420)
                     /locus_tag="LLD04_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649567.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="LLD04_002688"
     CDS             1628..2110
                     /locus_tag="LLD04_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649568.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="LLD04_002689"
     CDS             2307..3197
                     /locus_tag="LLD04_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK5649569.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1137 a          499 c          598 g         1048 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tg
//
LOCUS       DAFBYI010000181         3270 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFBYI010000181 DAFBYI010000000
VERSION     DAFBYI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.187"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..238
                     /locus_tag="LLD04_002690"
     CDS             <1..238
                     /locus_tag="LLD04_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649570.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="LLD04_002691"
     CDS             252..440
                     /locus_tag="LLD04_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649571.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="LLD04_002692"
     CDS             452..1063
                     /locus_tag="LLD04_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBK5649572.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="LLD04_002693"
     CDS             1048..2439
                     /locus_tag="LLD04_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5649573.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="LLD04_002694"
     CDS             2655..2957
                     /locus_tag="LLD04_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649574.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..>3270
                     /locus_tag="LLD04_002695"
     CDS             2950..>3270
                     /locus_tag="LLD04_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649575.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENK"
BASE COUNT         1287 a          456 c          634 g          893 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa
//
LOCUS       DAFBYI010000182         3232 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFBYI010000182 DAFBYI010000000
VERSION     DAFBYI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.188"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            184..1138
                     /locus_tag="LLD04_002696"
                     /pseudo
     CDS             184..1138
                     /locus_tag="LLD04_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            1155..2179
                     /locus_tag="LLD04_002697"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="LLD04_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="LLD04_002698"
     CDS             2185..2664
                     /locus_tag="LLD04_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK5649576.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3232
                     /locus_tag="LLD04_002699"
     CDS             2680..>3232
                     /locus_tag="LLD04_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK5649577.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          980 a          539 c          751 g          962 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cg
//
LOCUS       DAFBYI010000183         3220 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFBYI010000183 DAFBYI010000000
VERSION     DAFBYI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.189"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            50..1438
                     /locus_tag="LLD04_002700"
     CDS             50..1438
                     /locus_tag="LLD04_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBK5649578.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="LLD04_002701"
     CDS             1776..>3220
                     /locus_tag="LLD04_002701"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK5649579.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DAFBYI010000184         3189 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFBYI010000184 DAFBYI010000000
VERSION     DAFBYI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.190"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            204..1172
                     /locus_tag="LLD04_002702"
     CDS             204..1172
                     /locus_tag="LLD04_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5649580.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="LLD04_002703"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="LLD04_002703"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5649581.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="LLD04_002704"
     CDS             2202..2810
                     /locus_tag="LLD04_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5649582.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          945 a          610 c          777 g          857 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
     3181 ggaagattc
//
LOCUS       DAFBYI010000185         3171 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFBYI010000185 DAFBYI010000000
VERSION     DAFBYI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.191"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(786..1127)
                     /locus_tag="LLD04_002705"
     CDS             complement(786..1127)
                     /locus_tag="LLD04_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5649583.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1127..1357)
                     /locus_tag="LLD04_002706"
     CDS             complement(1127..1357)
                     /locus_tag="LLD04_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5649584.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1644..1982
                     /locus_tag="LLD04_002707"
     CDS             1644..1982
                     /locus_tag="LLD04_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649585.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2187..2426)
                     /locus_tag="LLD04_002708"
     CDS             complement(2187..2426)
                     /locus_tag="LLD04_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5649586.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2402..2749)
                     /locus_tag="LLD04_002709"
     CDS             complement(2402..2749)
                     /locus_tag="LLD04_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5649587.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1000 a          590 c          495 g         1086 t
ORIGIN      
        1 agtgactcaa aataaaaaaa aacttacatt atactggaat gaactgtatt gatagtgtac
       61 ctaaaagctc tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg
      121 gatgtaatca attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg
      181 ctttgagatt ctactttggt tcactatctg acccaattgc acattttcga tcattacaag
      241 tattggtgtc gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct
      301 aatttctgta tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc
      361 atgatatgcc tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt
      421 gatcccaaat tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta
      481 aagagtttag attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa
      541 gtattgtcgc gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca
      601 caaaaaaacg aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc
      661 tttagtttaa gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta
      721 cttggccttt ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac
      781 aatgcttaaa acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag
      841 ttttctattt ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt
      901 acttgtcctt ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata
      961 gtggaagtaa ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag
     1021 acgactagtc ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc
     1081 catacaatat cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc
     1141 tcccttcagg agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac
     1201 cactaaatgg atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa
     1261 cgatatattc ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta
     1321 cagaacttcc ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag
     1381 tatactatat aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt
     1441 ttgtttaaag tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt
     1501 ctttaactaa aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat
     1561 actatagcta accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt
     1621 caaagacaac aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt
     1681 gctctgacaa atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg
     1741 ttcggatcaa agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac
     1801 aagactttgc tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac
     1861 atgatccaac tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat
     1921 atacaactgc caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat
     1981 aatgataaac atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata
     2041 gaagctatac attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga
     2101 gtatttcttc tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc
     2161 gctctttctt tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc
     2221 tgagtaatga tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca
     2281 gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc
     2341 attatgattg attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac
     2401 actatttttt ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga
     2461 aaacacaacg attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg
     2521 cacgggcatt aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt
     2581 ccggagtgag gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa
     2641 cttctccagc tgctaattcc gctgcctgct tcggttgttt cgtctgggct tctcgataat
     2701 aattctgaag gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc
     2761 ccaatctttt tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa
     2821 tttttgtaga actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc
     2881 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa
     2941 ttgttccttg tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca
     3001 aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca
     3061 aaatttttct taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt
     3121 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag c
//
LOCUS       DAFBYI010000186         3027 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFBYI010000186 DAFBYI010000000
VERSION     DAFBYI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.192"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(106..1053)
                     /locus_tag="LLD04_002710"
     CDS             complement(106..1053)
                     /locus_tag="LLD04_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5649588.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="LLD04_002711"
     CDS             1299..1763
                     /locus_tag="LLD04_002711"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK5649589.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="LLD04_002712"
     CDS             complement(1825..2868)
                     /locus_tag="LLD04_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK5649590.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1002 a          599 c          580 g          846 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataag
//
LOCUS       DAFBYI010000187         2822 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFBYI010000187 DAFBYI010000000
VERSION     DAFBYI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.193"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            153..2750
                     /gene="adhE"
                     /locus_tag="LLD04_002713"
                     /gene_synonym="adhC"
     CDS             153..2750
                     /gene="adhE"
                     /locus_tag="LLD04_002713"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK5649591.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT          986 a          523 c          598 g          715 t
ORIGIN      
        1 tttctataca aacgcctata aataaagcaa ctttatattt aaaaataact gtgaaaaaaa
       61 tcacaaaaaa gtgtttacat agaaaaataa atattgtatg atatacatgt gaatgatgta
      121 acaaataacc atgacccatt aggaggataa tgatggctaa agtaatggaa aaagaaaaaa
      181 cgaagacaat tgacgtacaa gcaatgattg atgatttggc agaaaaagca aatgcagcat
      241 tgaaagaaat ggaaagcttt gatcaagaaa aagtggacca tatcgtgcat gaaatggcaa
      301 tggcagcttt agatcaacat atgccattag cgaagatggc tgtagaggaa acaggccgcg
      361 gtgtttacga agataaagca atcaaaaata tgtatgcgtc cgaatatatt tggcataata
      421 tcaaacatga taaaacagtt ggtgtaatca atgaagatgc ccaaaaagga ttgattgaaa
      481 ttgctgaacc agtcggtgtg gtttgcggag taactccaac aacgaatccg acttccacaa
      541 cgattttcaa atcaatgatc gcgttgaaaa caagaaatcc aatcgttttt gcttttcatc
      601 caagtgccca aaaatcttct gcagaagcag cacgagtagt acgtgatgct gctatcgctg
      661 ccggagctcc tgaaaattgt atccaatgga tcgaacatcc ttcgatcgaa gcgacttcta
      721 tgctaatgaa ccatccaggt atcgcgatcg tcttagcgac aggtggagct ggaatggtca
      781 agtctgctta ctctacagga aaaccagctt taggtgtagg tccaggtaac gtaccagctt
      841 atattgaaaa aacagcaaaa attaaacgtg cagtgaacga tttgatcgtt tcgaaaacat
      901 ttgataatgg aatgatttgt gcttctgaac aagctgtgat cgtggataaa gaaatctatg
      961 cagcagtaaa agcagaattc caagcacacc aagtttatat cgtaaaacca gatgaattgc
     1021 aaaaattaga agatgcagtg atgaacgaag gaaaatatgc ggtcaatcca tcaatcgttg
     1081 gacattctgc gatggaaatc gcaaaattgg caggaatcag tgtaccaaaa ggaacgaaaa
     1141 tgctgattgc tgaattggaa ggcgtaggcc cagattatcc gctatcaaga gaaaaacttt
     1201 ctccagttct tgccatgatc aaagctaaca acacggacca tgcgtttgat ttgtgtgaag
     1261 gtatgttgaa tcttggagga ttaggacata cagcagttat ccattcagaa aacgaagagt
     1321 tgcatgtcaa attcggtctt cgaatgaaag cttgtcgtat tttagtgaat acaccatctg
     1381 cagaaggcgg catcggtgat atctataacg aaatgattcc gtcattgact ttgggatgcg
     1441 gttcttatgg taagaactct gtttctcgta acgtctctgc tgtaaactta atcaatgtca
     1501 aaactgtagc gaaacggaga aataacatgc aatggttcaa attaccgcca aaaatcttct
     1561 ttgaaaagaa ctctctattg tacttagaga aaatggaaaa cgttgaacgt gtcatgatcg
     1621 tttgtgaccc tggtatggtc caattcggtt actgcgatac agttcgtgaa gtattggcac
     1681 gacgtaaaaa tgatgtgaag atcgaagtct tctcagatgt tgaaccgaac ccatcgacaa
     1741 ataccgtata cgcaggaacg aaactgatgg cagacttcaa gccagataca gtaatcgctc
     1801 ttggaggtgg ttctgcaatg gacgcagcta agggaatgtg gatgttctat gaacacccag
     1861 acacatcttt ctttggtgca aaacaaaaat tcttggatat ccgtaaacgg acatataaga
     1921 tcgacaaacc agaaaaaaca caattcgtat gtatcccgac aacatcaggg actggttctg
     1981 aagtaacgcc atttgcggtc atcactgaca gtgaaacaca tgtgaaatat ccgttagcag
     2041 attatgcatt gacgccagat gtcgcaatcg tcgatcctca attcgtcatg tcagttccag
     2101 catctgtcac agcagatacc gggatggatg tgttaacaca tgcaatcgaa tcctatgttt
     2161 cagtcatggc ttcagattat acacgtggat tgagcctaca agcaatcaag ttagtctttg
     2221 actatttaga aaaatcagta aaaacacctg atatggaatc acgtgaaaaa atgcacaacg
     2281 cttctacaat ggctggtatg gcgtttgcta acgcattcct aggtatttgt cactcagtcg
     2341 cacataaaat cggcggggaa tacgggatcc ctcatggacg gacaaatgcg attcttttac
     2401 cgcatattat ccgctataat gcaaaagatc cacaaaaaca tgcgatgttc cctaaatatg
     2461 actatttccg tgcagataca gattacgcag atattgcaaa attcttaggt ctgaaaggcg
     2521 aaacaacaga ggaattagtc gaagctttag caacggctgt ttatgaatta ggaaaatctg
     2581 tcggcatcaa catgagtcta aaagctcaag gagtcacaca agaaacacta gatacaaccg
     2641 tagaccgtat ggctgaatta gcttacgaag accaatgtac gacagctaat ccgaaagaac
     2701 cattgatctc tgaattgaaa cagatcatca ttgatgctta caacggataa gcaaaagaaa
     2761 gcgtagaaaa aagataagaa aatagatgaa aaagaacttg gaaaacgttg tttctccaag
     2821 tt
//
LOCUS       DAFBYI010000188         2815 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFBYI010000188 DAFBYI010000000
VERSION     DAFBYI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.196"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..75)
                     /locus_tag="LLD04_002714"
     CDS             complement(<1..75)
                     /locus_tag="LLD04_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK5649592.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYN"
     gene            complement(149..430)
                     /locus_tag="LLD04_002715"
                     /pseudo
     CDS             complement(149..430)
                     /locus_tag="LLD04_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            892..2274
                     /locus_tag="LLD04_002716"
                     /pseudo
     CDS             892..2274
                     /locus_tag="LLD04_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2510..>2815)
                     /locus_tag="LLD04_002717"
     CDS             complement(2510..>2815)
                     /locus_tag="LLD04_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5649593.1"
                     /translation="RLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGG
                     VMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          983 a          507 c          477 g          848 t
ORIGIN      
        1 attgtaacgc aggtagtaac caacagcgac aataatgacg tcttttttga attgtttgcc
       61 tttaaaatga ttcattactc tgtcctctct gtcttttttc tcaattttac actaaaatag
      121 attttttgga aaactttgca acagaaccga aatgagtata gtatgaatta gtaatttggt
      181 tttcatctaa gtttagcttt gatttactaa aattgatatc tcgatacatg ttcaatacat
      241 ctaatacaaa tcgagaatct tctttcggaa atggctcaga aagatcactc caaatttcat
      301 catcataatg atactcgtaa ccttctctca atatttcgat ttttctttca taataagatc
      361 tgttttggtc ctcaaattta gccaataact caaattgatt tactagtgac aatctctgaa
      421 aactgtccat taaatgtttc ttcctatctt gttcattaac aaaaaattat aagcccttac
      481 aacgaatatt atatattaaa tttaattgta ggtgaacacg aattttataa atatttatgg
      541 tgttaatgac aaatgatatc cgactcctct tcatacgttc tggactattt gctaatagat
      601 taaaatttca gcatctattt ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc
      661 agacttgaaa ataaaggatc aaaaactttt aagtttctga ccccgtaact cacacttcaa
      721 aatcttactt aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta
      781 gctacatcac atctatgcat tgacactcaa agaaccagaa taagtcatca gaaagcaagg
      841 gggtgcggac ataatcctgg acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc
      901 cacaaagagc gtgtaagggc aattcagtta tttttaaaat acgattgctc ttatgctgca
      961 accattcgag aattagggta tccgtcgatt ggtgcactac ggaaatggta taaagagtat
     1021 ttggaatccg gagagctcca tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa
     1081 aaacgaatag cggtgaatca ttattttgaa tatggtcaat gttatgcacg aaccattcgc
     1141 atgcttggtt atcctaatag agagtccttg cggcagcggt gtgaagaact tgcaccagga
     1201 gctagaaaac tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg
     1261 aaaaaattct ataaaccgca aactaatagg aaaaatttag ccgaatctgg aggaattagt
     1321 agagttactc tatatcaatg gaaaaacacg gttcttggaa aggattttcc attgtgtatg
     1381 actttaaaaa atcaggaaaa acaaaaagag ctacttttaa aggaagtaga agacttacaa
     1441 aaacaagtac accaattaca gctcgaaaaa gcattactcg aaggagcggc tgaactgcta
     1501 aaaaaagaaa aaggcgtcaa tcttctttgt ctatcaaatc aagaaaaaac gatattgatt
     1561 gatgccttac gaaatcagtt caccttgaaa gagcttcttc aacagctcca gtttcctaaa
     1621 agcagctatt tttatcaaaa acaagcactt gagaaaccag ataagtatta caaagaacgt
     1681 caaattaatt atcacgattt tcaatcataa cttctgtgcg tatggttacc gtagaattca
     1741 ccaagcttta aaaaatatgg gtaaaaagct ttctgaaaaa gttgtacgta gattgatgac
     1801 tgaggaaaat ctatttgtta aattttctag acgaaaaaag tattcttcgt atgctgggga
     1861 aatttctcct gcacacccta atttattaaa tcggaatttt aaagcaaaga agccaaatga
     1921 aaaatggtta acagatatta ctgaatttag aattctcgca gggaaaatct acttatcacc
     1981 acttgtcgat tgttacgatg gtgctattgt tagttggact attggaacaa agccaacttg
     2041 cttcactcta tgtctaaaaa gggttattca ccagataatt cagcgtgcga aggtttcttt
     2101 ggacgattaa aaaacgagat gttttatcaa cgtgactgga agaatacaac aatcaatcag
     2161 tttataaaca aattggatga ttatattcat tggtataata atcaacgaat taagttatct
     2221 ttaggagggc taagccctct tcaatatcgg aagaagcaaa gatatatttg ttaaaaaggt
     2281 ccaaaaaaaa tatccgcacc cccttttttt tgtaaaaaac tattgcacaa tacaagtagc
     2341 ctacggctat cgaaatttcc ataactttcg agcaagaact tatctcattc aaggcttgat
     2401 cttccaagta aaacaaaaac cagttaagca ctctgcctaa ctggttatga ctaaattttc
     2461 agttgtatct ttctctacca acactaaatg tcgaagagcc ttttttcgtt tatgaatgta
     2521 agaccgcttc tcgagccaat gccaaacaac cgagcgttcc agcgttgtct tctaatgctg
     2581 gggtcacgat atatttgctt aactcaggtg tttcgacata cccattgacc aattttgcaa
     2641 atgcgtcgtg gacgttttgc agcatatggc gttgtttcat cacaccaccg ccaaaaatga
     2701 tcacatctgg agaaaacatc aaggttgtat tgtaggcaca ttgagcaata taggaggctt
     2761 cgatttccca ataaggatca tcttctgcaa cctcttgtcc ttttttccct aagcg
//
LOCUS       DAFBYI010000189         2784 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFBYI010000189 DAFBYI010000000
VERSION     DAFBYI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.197"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1023
                     /locus_tag="LLD04_002718"
     CDS             <1..1023
                     /locus_tag="LLD04_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5649594.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="LLD04_002719"
     CDS             1157..2500
                     /locus_tag="LLD04_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5649595.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="LLD04_002720"
     CDS             2612..>2784
                     /locus_tag="LLD04_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649596.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          517 g          795 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctgggaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DAFBYI010000190         2781 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFBYI010000190 DAFBYI010000000
VERSION     DAFBYI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.198"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..89
                     /locus_tag="LLD04_002721"
     CDS             <1..89
                     /locus_tag="LLD04_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK5649597.1"
                     /translation="LYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            89..340
                     /locus_tag="LLD04_002722"
     CDS             89..340
                     /locus_tag="LLD04_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649598.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            356..511
                     /locus_tag="LLD04_002723"
     CDS             356..511
                     /locus_tag="LLD04_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649599.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            521..793
                     /locus_tag="LLD04_002724"
     CDS             521..793
                     /locus_tag="LLD04_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649600.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            1238..1417
                     /locus_tag="LLD04_002725"
     CDS             1238..1417
                     /locus_tag="LLD04_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649601.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1476..1832
                     /locus_tag="LLD04_002726"
     CDS             1476..1832
                     /locus_tag="LLD04_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649602.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1014 a          386 c          534 g          847 t
ORIGIN      
        1 gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt
       61 taattgatga gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa
      121 agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagtttt
      181 acttattccg cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct
      241 atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac
      301 ttatgaaaac gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc
      361 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
      421 ggatatgacg ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt
      481 gtcggtagct gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta
      541 ctatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact
      601 atgcttggaa gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga
      661 actaacgtat gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt
      721 agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa
      781 cgaaaatgaa taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga
      841 aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa
      901 cgcctagtaa ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg
      961 cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca
     1021 tttgaacaag attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga
     1081 tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat
     1141 cttttggcgg tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt
     1201 tttcataggg ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa
     1261 acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa
     1321 gtatggcaaa agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga
     1381 aaaagcagga tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat
     1441 ataagtgata tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt
     1501 acaaattaaa gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc
     1561 atttgtatag agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag
     1621 ctggggatcg atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc
     1681 tttggcttat tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact
     1741 tagataagct aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt
     1801 tagaaagtga cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat
     1861 tattttatta agaatgatgg aatataagga taaggttacc aacataacga tcattacttc
     1921 tctgatatgc tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat
     1981 actgcaagcc ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat
     2041 atataagcta tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag
     2101 ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag
     2161 taggatttga catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg
     2221 gagataagat gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac
     2281 cgcaactgaa gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg
     2341 ttaccttaaa atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga
     2401 tggggtacgt gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat
     2461 aaatttagac atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat
     2521 tatagtaatg attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa
     2581 aattgagaaa tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat
     2641 gaagcttacc aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc
     2701 tttgaggaga tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg
     2761 cggtggaggt aaaggttttt a
//
LOCUS       DAFBYI010000191         2778 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFBYI010000191 DAFBYI010000000
VERSION     DAFBYI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.199"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..197
                     /locus_tag="LLD04_002727"
     CDS             <1..197
                     /locus_tag="LLD04_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649603.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LLD04_002728"
     CDS             314..811
                     /locus_tag="LLD04_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5649604.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LLD04_002729"
     CDS             898..1290
                     /locus_tag="LLD04_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5649605.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="LLD04_002730"
     CDS             1274..>2778
                     /locus_tag="LLD04_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5649606.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DAFBYI010000192         2778 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFBYI010000192 DAFBYI010000000
VERSION     DAFBYI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.200"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(748..1041)
                     /locus_tag="LLD04_002731"
     CDS             complement(748..1041)
                     /locus_tag="LLD04_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK5649607.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1299..1646)
                     /gene="rplS"
                     /locus_tag="LLD04_002732"
     CDS             complement(1299..1646)
                     /gene="rplS"
                     /locus_tag="LLD04_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK5649608.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(1782..2543)
                     /gene="trmD"
                     /locus_tag="LLD04_002733"
     CDS             complement(1782..2543)
                     /gene="trmD"
                     /locus_tag="LLD04_002733"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK5649609.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            complement(2533..>2778)
                     /gene="rimM"
                     /locus_tag="LLD04_002734"
     CDS             complement(2533..>2778)
                     /gene="rimM"
                     /locus_tag="LLD04_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBK5649610.1"
                     /translation="DLAENEFYYHEIIGLHVLEDGTELGKVKEILSPGANDVWVVQRP
                     KKADILLPYIASVVKEVDLEAGVVRVEIPEGLIDDED"
BASE COUNT          806 a          536 c          447 g          989 t
ORIGIN      
        1 tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa gattttattt
       61 ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa cttgattaat
      121 tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag gacgggatta
      181 tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga gaaatgtgtc
      241 gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caattagctg atcttgtatt
      301 cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc taacgctcag
      361 gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt ttcaagaacg
      421 aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga ggtacgtttt
      481 ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca tccaattgat
      541 tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac tgcgaatcgc
      601 tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt tgtattatag
      661 atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca tacttgaact
      721 tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct gatatgcccg
      781 accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt gttcgacata
      841 atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg tataagtcac
      901 tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt ttgttgaact
      961 tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca tcttttcttg
     1021 ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac tccatttatg
     1081 ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat tatcatagtg
     1141 ctcttttttt tactactact tataggtaga tttattctaa tccattattc tcccctacca
     1201 ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa aaagaaccct
     1261 tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat ttctttgata
     1321 cgagcagctt ttccgtgtaa tgcacgtagg tagtacaatt ttgcacgacg tactttaccg
     1381 tagcgaacta cttcgatttt tgcaacacgt ggtgtatgca atgggaatgt acgttcaacg
     1441 cccactccgt tagaaacttt acgtactgta taagtttcgc tgattccagc accgcggcgt
     1501 ttgataacaa caccttcaaa taactggata cgttcacgag taccttcaac aacttttgcg
     1561 tgaacacgca cagtgtcacc agggcggaac gctgggatgt cagaacgtaa ttgttctttt
     1621 gttaattctt cgattaatgg attcatggtt ttcttctcct tattccaaat attcttatga
     1681 gcttttctca cagcggaata tcgtaatagt taagtgcttt tgcactcacc ataaaatact
     1741 aacatattat caacctactg taaagaagat tttagaaaaa gtcacttggc ttcatctgct
     1801 gttttttctt gttcttcttt cttgatttct tctaaaaatt tcgtcatttg aggtgtccat
     1861 tcaagtgttt ccaaaagatc aggccgacgt tgataggtcc ttcttagtga ttcttttaat
     1921 tgccattctt caatcaattt atgatttcca tttgtcaata cttctggtac tttcatccct
     1981 ttatattctg ccgggcgagt atattgggga tgttccaata aaccggttga ataagaatcc
     2041 gtttgagctg aagtctgatt ccctaacaca tctggcaaaa gccgaactgt tgcatcgatc
     2101 atgaccatcg cacctaattc tcccccagta aggacataat ctcctaagga aacttcatct
     2161 gtcactaatg tgcggatacg ttcatcatat ccctcataat gaccacaaat gaatactaag
     2221 tgttcttctt gtgaaaattc ttctgccatt ttctgatcga atcgtttacc agctgggtca
     2281 agaagaatta ctcttttttt gacatccggt gcagcttttt caatggcttg gatgttatca
     2341 tagatgggct ggactttgag cagcatacct gctccaccgc cgtatggata atcatctact
     2401 gtctgatgtt tgttatctga ataatcgcgg aagttagaga tattgatatc taataatcct
     2461 ttatctacag ccttaccgat gatggattcc cccataggac cttcaaacat tcttggaaat
     2521 aaagtcaata catcaatctt catcgtcaat cagtccttct ggaatttcta cacggaccac
     2581 tccagcttct aaatcgactt ctttgacgac agaagcgata tatggaagca atatatcagc
     2641 ttttttcgga cgttgaacga cccaaacatc attggcacct ggtgacaaga tttcttttac
     2701 tttacctaac tcagttccat cttctaatac atgcagaccg atgatctcat ggtaatagaa
     2761 ttcattttca gctagatc
//
LOCUS       DAFBYI010000193         2742 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFBYI010000193 DAFBYI010000000
VERSION     DAFBYI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.201"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(45..1364)
                     /locus_tag="LLD04_002735"
     CDS             complement(45..1364)
                     /locus_tag="LLD04_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5649611.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LLD04_002736"
     CDS             complement(1361..2014)
                     /locus_tag="LLD04_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5649612.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2673
                     /locus_tag="LLD04_002737"
                     /pseudo
     CDS             2302..2673
                     /locus_tag="LLD04_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          915 a          437 c          282 g         1108 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac ga
//
LOCUS       DAFBYI010000194         2625 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFBYI010000194 DAFBYI010000000
VERSION     DAFBYI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.202"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="LLD04_002738"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="LLD04_002738"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5649613.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaa
//
LOCUS       DAFBYI010000195         2527 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFBYI010000195 DAFBYI010000000
VERSION     DAFBYI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.203"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..321
                     /locus_tag="LLD04_002739"
     CDS             <1..321
                     /locus_tag="LLD04_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649614.1"
                     /translation="ENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEILLCLA
                     MQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVMTRILK
                     GEFQ"
     gene            554..1495
                     /locus_tag="LLD04_002740"
     CDS             554..1495
                     /locus_tag="LLD04_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK5649615.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKLVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            1497..2384
                     /locus_tag="LLD04_002741"
     CDS             1497..2384
                     /locus_tag="LLD04_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK5649616.1"
                     /translation="MATNESLKKQLTEQNTQVVDPSKLGFKALMSTPAMKKKFTDILH
                     EKSDSFMGSLMTLVGGDNYLSKAEPMTIIASALKAATMDLPIDKNLGYAYVVPFNRKE
                     KVGNEWITHNEAQFILGYKGYIQLAQRSGQYKALNALEIYEGQLIDWNPLTEEFQFDY
                     KAKQSDTVIGYVGFFELLNGFKKTVYWTKQEIESHRIKNAKGFDKEKLTGAWKDNYDA
                     MAIKTVLRNLLSKWGILSVEMQTAVTSDEKVFRLDENENLIEETTLSEVEPELKEAEP
                     VAEDVQTGLFDASNPPLNK"
     gene            2427..>2527
                     /locus_tag="LLD04_002742"
     CDS             2427..>2527
                     /locus_tag="LLD04_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5649617.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKA"
BASE COUNT          926 a          398 c          525 g          678 t
ORIGIN      
        1 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
       61 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
      121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
      181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
      241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
      301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
      361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
      421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
      481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc gccaattgat
      541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggctcgaaga
      601 ccgcaaaaga ggtattggtg gctcagatgt tgcaaccgta cttggattaa acaaatacaa
      661 atctccttat caattgtggc ttgagaaaac gggtcaaatt gaattgaaag atttagaaag
      721 cgaaccagct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
      781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
      841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gtaaaacagc
      901 caatcaattt ctcggaaaag agtgggaagg tgaagaggta ccgcttagct atctctgtca
      961 agttcagcat tacatgaacg ttttaaacaa agactattgt tatatcgctg ttttgatcgg
     1021 tggacaaaaa ttcatttgga agcggattga acgagatcaa gagctgatcg atacaatcac
     1081 tgaacaatta gtagagtttt gggaaacgaa cgttcttgga ggtatcgagc ctgtaattga
     1141 cggaagtgaa gcgactgctg atttcttaaa agaaaaatat gcagatgtag aagaaaatca
     1201 aacagctcta ccatcacgtt ttgatgaact tatcgagcaa aaaagagaac tcaagcggac
     1261 gaaaaaagaa attgaatcag ctatccgtca agtagacaac gagatcatca gtgagctagg
     1321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aacttgtccg
     1381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     1441 agagatttat aacgttactg aatcaagaag gctaaccgaa aaggagatca aataatatgg
     1501 caacaaatga atcgttaaaa aaacaactga ctgaacaaaa tactcaagtg gttgacccat
     1561 caaagttagg ctttaaagct ttgatgagta cgccagcaat gaagaaaaaa tttactgata
     1621 ttctacatga aaaatcggat tcttttatgg gatcgctcat gactttagtt ggtggcgata
     1681 actatctatc taaagctgaa ccaatgacca ttattgcttc cgcactaaaa gcagcaacta
     1741 tggatctacc catcgacaaa aatttaggat atgcctatgt tgttccattc aatagaaaag
     1801 aaaaagttgg aaatgaatgg attacacata atgaagcaca atttatcttg gggtataagg
     1861 gctatattca gctagcacaa cgatctggtc aatacaaagc gctaaatgct ttagaaatat
     1921 acgaaggtca gttgattgac tggaatcccc tcacagaaga atttcaattc gattataaag
     1981 ccaaacaatc tgatacagtc atcggttatg taggtttctt cgaattatta aatggattca
     2041 aaaaaacagt ttattggacc aaacaagaaa ttgaaagcca tcggatcaag aacgctaaag
     2101 gctttgacaa agagaaatta acaggtgctt ggaaagataa ttacgatgca atggctatta
     2161 agacagtatt acgtaacctt ctttcaaaat ggggaatttt gtcagtagag atgcaaacgg
     2221 cagttacttc agatgagaaa gttttccgat tagatgaaaa cgaaaatttg attgaagaaa
     2281 cgactttgtc agaagttgaa ccagaactaa aagaggcaga acctgttgca gaagatgtac
     2341 aaactggatt atttgatgca tctaatccgc cgttaaacaa ataatgaggg agctttctcc
     2401 cttgcttttc tagaggagaa atacgaatga acacaggata tgtcaaatta catcgaaaag
     2461 tgatgaattc attcgtttgg accaatcctt atatgtataa attatggagc ttgtgtttga
     2521 tgaaagc
//
LOCUS       DAFBYI010000196         2494 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFBYI010000196 DAFBYI010000000
VERSION     DAFBYI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.204"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            282..464
                     /locus_tag="LLD04_002743"
     CDS             282..464
                     /locus_tag="LLD04_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649618.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            448..633
                     /locus_tag="LLD04_002744"
     CDS             448..633
                     /locus_tag="LLD04_002744"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBK5649619.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            876..2396
                     /locus_tag="LLD04_002745"
     CDS             876..2396
                     /locus_tag="LLD04_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5649620.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          861 a          420 c          498 g          715 t
ORIGIN      
        1 ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat agttgattta
       61 ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa caactatatt
      121 aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct aatttgaggc
      181 aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc gattatcttt
      241 cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa aaagatattg
      301 taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag ccaaaactag
      361 cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg gataaatatc
      421 ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac tgcattgaaa
      481 catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg actagcagct
      541 ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt attttgcggt
      601 acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt attaatgtac
      661 aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt cttactgact
      721 ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc gatatattag
      781 acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa atggtataat
      841 agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa tctgaacttc
      901 ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa aatactcttc
      961 ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa gaaacaccac
     1021 ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca gagcacactg
     1081 gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa cgcaagggac
     1141 tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa ttaaaaggtg
     1201 ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg acaaggctta
     1261 gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac caacatgcct
     1321 atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg cctgttccta
     1381 aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt atttgggaaa
     1441 aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga atggcatgca cttggtttcc
     1501 ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg gttttagagc
     1561 caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac gcggatgaga
     1621 caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta tatgcttcag
     1681 gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt tccgttgatg
     1741 agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt tacgcggcct
     1801 atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga aagttttttg
     1861 aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta gacttctgtg
     1921 acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa aggtttgtag
     1981 cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt gagagttttg
     2041 cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat cataaagacg
     2101 gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga gcagagcgtg
     2161 ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc ttaaaaggcg
     2221 ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat gggttaaaca
     2281 tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg atgacacctg
     2341 aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt aaataatata
     2401 agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta ggtaccattc
     2461 tatgacacgc ttacgctcaa aactactgaa ttat
//
LOCUS       DAFBYI010000197         2484 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFBYI010000197 DAFBYI010000000
VERSION     DAFBYI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.205"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(1015..1305)
                     /locus_tag="LLD04_002746"
     CDS             complement(1015..1305)
                     /locus_tag="LLD04_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649621.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="LLD04_002747"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="LLD04_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          735 a          459 c          347 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga ataattctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DAFBYI010000198         2468 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFBYI010000198 DAFBYI010000000
VERSION     DAFBYI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.206"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(493..1785)
                     /locus_tag="LLD04_002748"
     CDS             complement(493..1785)
                     /locus_tag="LLD04_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5649622.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="LLD04_002749"
     CDS             complement(2059..2319)
                     /locus_tag="LLD04_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649623.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DAFBYI010000199         2397 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFBYI010000199 DAFBYI010000000
VERSION     DAFBYI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.207"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            206..2068
                     /locus_tag="LLD04_002750"
     CDS             206..2068
                     /locus_tag="LLD04_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5649624.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="LLD04_002751"
     CDS             2068..>2397
                     /locus_tag="LLD04_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5649625.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAFBYI010000200         2348 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFBYI010000200 DAFBYI010000000
VERSION     DAFBYI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.208"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(237..2234)
                     /locus_tag="LLD04_002752"
     CDS             complement(237..2234)
                     /locus_tag="LLD04_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK5649626.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          609 a          521 c          425 g          793 t
ORIGIN      
        1 gattttgcac aactcatcag tccttattga caaagagccc ttaaactata atagagaatt
       61 agattccaca aaaaagaggt tgtgacaatt tttgtcacaa ccttttttgc cgagtaaacg
      121 gtgttcaaaa gccagtttct cggtatgaag tcaaatttaa gcaaaaatgt gagaagctat
      181 tttcacaaaa tttttcttca tactcgaaac taagcggctt tttcacgacc tcttttttat
      241 tttgccatga acatatcata atacgcgtcc attatttctt tagcaatcgt caagtttatg
      301 tggtcttgtt cgtctaataa gttcggcaag acaacactga tggcaatttc cggattgtct
      361 gttggtccgt atgccacgat attactattg actgtcgcaa tatctggatg accttccgca
      421 accgtttctg cagtccctgt ttttgcggaa aggtccattt tcgcactggc taatggtttg
      481 gctgttgtgt aaggatcggt accatggaca acttggtgga atccttcacg gaggatcgcc
      541 atattttctg cagaaatatt gaccgtattt tctactgttg tgccaatttc tttcttcact
      601 tcacccaagt ttccattttc gtcattgcca tagatcgctt tgactaggtg cggctgtact
      661 cgtttcccgc cattagcgac tgttgaagca tattgagcta attggattgg tgtatatgtg
      721 tcaaactgtc caaatgccaa atcggtaaag ttacctggag cataccattc tttgccatta
      781 tccgcattga acttcttcat ataatcaaca gaacgtgaga tcccaggaga ttcattcggc
      841 aaatcgatcc cagtcgttgt accaagacca aactctttga aagagtctct caattgctga
      901 taagctttag cttcttcatc taaactaggt agtcccattc caggtgtata gtctagtcct
      961 aacatcttta acgccacttt gatcatatac gtattagaag acaattccga tgctttgact
     1021 gcatcaagag caacttgtcc gcttcgatta aagacagagc ttttttctga agcaccttgc
     1081 agacgaatcg gctcatcaat caaaacttga tttccgctga tcgcgttact tgcccagcct
     1141 gctgtcaaag taccagcctt gactacagac cctggatcaa aggcgctagt aatcgtgcct
     1201 aaagcattct cggtgatttc tttggaacct tgttcatgac tgaaaccagt cattgctagc
     1261 acttctcctg tctgaggatt catagccact gcataagcac caggcgaata ctgtgctttg
     1321 ccgttgttga tcaatgtttg ataattcctt ttcaaaattt cttctacttt tgattggaat
     1381 tctgcattca tagataacat taaattagaa cctttttccc cagagaagat ttctttttga
     1441 ttcgaaacat ttccttcatt atcaagggaa acttcatatt gggttttggt tccttgcagg
     1501 acatcttcat attgtttttc caaataactt tgtcctacac ggtcattccg agaataacct
     1561 tttgctaggt atttctctgc ttcatctgct ggaagtcctt gcttttcagt cgtcacactg
     1621 ccaagaatac ttttcagtga acttgctgca ttatattctc gtgtccagtc tgtgccagtc
     1681 gaaactcctg gaagttcaga agcgcgttct gccacaacag ctaactcttg atcagtaaca
     1741 ccagagtttt tgatatagac agtgtttaat tcatatgcac tattcatccg tttaaagatg
     1801 gtagcaattt ttaattgctc ctcgtttaaa ttaatctcat cagctgtgac tttattgacc
     1861 atcgcactat attgttcgct gttccctaat tgcttttctt ttgccgaaag tctttttgtg
     1921 gcttctttca aatgtttggg atcagccagc caaaaatctt ttttatctct atctgtcaga
     1981 ttttcatcta ccggaacatc gatcagcgag ttcagcttcg ttgcgatttc aagcaaatct
     2041 ttagccgtaa cttgtgttcc tctcgtatat gtgatggcct gattcgcttt attcgtaact
     2101 aaagcttttc ctgtggagtc gtagatcatt ccgcgaggag tactttcttg gaccgtaatc
     2161 gtagaagtag acttcactcg cgcctccatc tcttcgccgt tcacgatctg caaataaccc
     2221 aatctagcaa tcaacgcaac aaataacgta aatataatga aaaaaagaaa atttaagcga
     2281 aaaggaacat gggaccggcg aaacgttttg ttcttaggtt cttgtgcacc ttttcttatt
     2341 ttttctaa
//
LOCUS       DAFBYI010000201         2326 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFBYI010000201 DAFBYI010000000
VERSION     DAFBYI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.209"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            79..1125
                     /locus_tag="LLD04_002753"
     CDS             79..1125
                     /locus_tag="LLD04_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK5649627.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="LLD04_002754"
     CDS             1474..2106
                     /locus_tag="LLD04_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBK5649628.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          868 a          314 c          459 g          685 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatg
//
LOCUS       DAFBYI010000202         2230 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFBYI010000202 DAFBYI010000000
VERSION     DAFBYI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.210"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(94..390)
                     /locus_tag="LLD04_002755"
     CDS             complement(94..390)
                     /locus_tag="LLD04_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK5649629.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="LLD04_002756"
     CDS             746..1102
                     /locus_tag="LLD04_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5649630.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="LLD04_002757"
     CDS             1112..2011
                     /locus_tag="LLD04_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5649631.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DAFBYI010000203         2094 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFBYI010000203 DAFBYI010000000
VERSION     DAFBYI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.212"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..339
                     /locus_tag="LLD04_002758"
     CDS             <1..339
                     /locus_tag="LLD04_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK5649632.1"
                     /translation="KTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQERFELMPKF
                     ELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQVPLILAKK
                     YRNTFKEERF"
     gene            354..515
                     /locus_tag="LLD04_002759"
     CDS             354..515
                     /locus_tag="LLD04_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5649633.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            512..838
                     /locus_tag="LLD04_002760"
     CDS             512..838
                     /locus_tag="LLD04_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649634.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            835..1650
                     /locus_tag="LLD04_002761"
     CDS             835..1650
                     /locus_tag="LLD04_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBK5649635.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            1789..>2094
                     /locus_tag="LLD04_002762"
     CDS             1789..>2094
                     /locus_tag="LLD04_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK5649636.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     "
BASE COUNT          754 a          324 c          530 g          486 t
ORIGIN      
        1 aaaactgttc atcgaggtat caagtttgat tctatcgcgg aagcagagta ttacgatcta
       61 gccttgtggc aagctgaagc gaatggttgg aaagtgaagc tccaggaaag gtttgagctg
      121 atgccgaagt ttgaactaga gggcaagaag tatcgcaaga tcgagtatat tcccgacttc
      181 acattttaca aaagcggcaa gctggtcaaa gtcgtagatg ttaaggggat gcagacaaaa
      241 gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagcc
      301 aaaaaatatc ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa
      361 cagaagaagt gattcaaatg cgaattcgaa gccttcggcg tgagattgac gatctggaac
      421 gaacaaaggc agtgatggtc aatgaaacgg ctagaaaggc aatcgatttg cacatagaga
      481 atttaagaag ggaaatccgt agattggagg aatgagcgtg gataaggaag cggcttggcg
      541 aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag tggttgctga
      601 agttcagcgt ctaggaaaga ttgctaacgg acgtatacga aaaaagccag acaaaagaaa
      661 acagcgcaaa gggaaaatcg tcgttgtttt acacgaaggc aaaattttga tgcaaggaac
      721 agctagtgag ctgtctgcag aaactggata tacgcgtggg actattcgaa cgtacgcttg
      781 gcgaaatcac accgatcgaa aagggcacga atataagtat ttggaggaag aaaaatgaac
      841 gaaaacaaat taatcaaatt gggtgtagca ggagcagtaa tcgtaggtat tggagttatc
      901 ggaggattta agttcttcga aaaaatcgat aatggatatg tgggtgtgcg ctattcaatg
      961 aacggcggta tcaaagatga agcactgacg caaggtgtga aatttgtagg gattgacaaa
     1021 gtgatccaat atccaattcg cttgcaaact atccaatcaa aaaatatttc agtatctaca
     1081 agcgacggca aaaagacaac gattgatatc aaatatgact acaaagttga ttcaactaaa
     1141 gcagcaaaaa tgtacaagga atttgggaat atcacttcgg aagatatcga aagtggatgg
     1201 ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg cgaaatatag tctgcttgat
     1261 gtcctttcag gagattcctc taaagttgaa gctgaggtat taacgaactt tgctaaatca
     1321 gttgaatcta aagggtttga agtcgaagac gtaacacttg gtgttccaga tgtcgataaa
     1381 gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc aagaaaatga aaaagcgaag
     1441 ctagacgcag aaactgcaaa aactcaagct gatagtgaag cttacaagaa aacaaaagct
     1501 gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat cagtaacaga caatttgatt
     1561 cgttatgaag aagctcaagc tcgcaaaaag catggatggg taacagtaaa tggagcagat
     1621 actgtagtta cggatgaagc aggcaaataa tatgggattc tttatggcta aaattctctt
     1681 gttcttaggt ttagtaggag cagcatatct cgtgtatgcg ctcctttccc aaactgatga
     1741 caaagaagat gacaacaacg atgaaatgaa attttaggag gagaaataat ggacgaacta
     1801 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcaga tccaaaagca
     1861 cagtttttga aagtagctga ggaattcggg gaaatagctt cggcgatggc aagaagtaat
     1921 gatgaactat ttaaagatag cgtaggagac gttatcgtca cgctgattat cctttccatg
     1981 caaaaaggga caaacgtaca agagtgttta gaaatggcat acaacgaaat caaaggacgc
     2041 acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga tgtg
//
LOCUS       DAFBYI010000204         2034 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFBYI010000204 DAFBYI010000000
VERSION     DAFBYI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.214"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="LLD04_002763"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="LLD04_002763"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK5649637.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="LLD04_002764"
     CDS             complement(1431..1823)
                     /locus_tag="LLD04_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5649638.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DAFBYI010000205         2024 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFBYI010000205 DAFBYI010000000
VERSION     DAFBYI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.215"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..424)
                     /locus_tag="LLD04_002765"
     CDS             complement(<1..424)
                     /locus_tag="LLD04_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5649639.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="LLD04_002766"
     CDS             complement(533..1564)
                     /locus_tag="LLD04_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5649640.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          626 a          330 c          347 g          721 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataa
//
LOCUS       DAFBYI010000206         1969 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFBYI010000206 DAFBYI010000000
VERSION     DAFBYI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.216"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..243
                     /locus_tag="LLD04_002767"
     CDS             <1..243
                     /locus_tag="LLD04_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649641.1"
                     /translation="FNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEEL
                     TEKADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            243..380
                     /locus_tag="LLD04_002768"
     CDS             243..380
                     /locus_tag="LLD04_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5649642.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDEEADKIISKEESA
                     S"
     gene            418..711
                     /locus_tag="LLD04_002769"
     CDS             418..711
                     /locus_tag="LLD04_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649643.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            708..932
                     /locus_tag="LLD04_002770"
     CDS             708..932
                     /locus_tag="LLD04_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5649644.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            929..1954
                     /locus_tag="LLD04_002771"
     CDS             929..1954
                     /locus_tag="LLD04_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649645.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVDGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPQR"
BASE COUNT          703 a          303 c          402 g          561 t
ORIGIN      
        1 tttaacttca ctgaaaataa gtgggaagaa gcagttacac aagattattc aaaaaaatta
       61 gaactactag aaaatttatc tataggttta caggtagata atgctgcatt gaaaaaatca
      121 aatgaagagt taacggaaaa agcagattcg atggcgcagt tgaacgccaa actgatgctt
      181 aatgatttaa caattaataa aaaaattgaa gcaatagaaa aacaaattgg aggtgcttca
      241 taatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
      301 aagttcgaca attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
      361 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
      421 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
      481 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
      541 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      601 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
      661 gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa
      721 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
      781 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
      841 gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct
      901 cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag
      961 cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga
     1021 gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt
     1081 gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta
     1141 taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt
     1201 gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca
     1261 gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat
     1321 tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac
     1381 ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa
     1441 ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta
     1501 tgatttagct catggattta ccgatgaaaa tccaactact tcagatgata aaccagtcat
     1561 tgatccaact agagcaggtg cagcaaatcc tacgctgaca gatggaacaa atcacgccca
     1621 cattgatcag tttggagaaa tcgaaaacgc aaacttgcat gtggatggat ggcacattgc
     1681 taattataaa tacgagtata ttttcattat ggattacaat actggaaaag aattagctcg
     1741 agtaagagct gatggaattt atagaccaga tgtaaatcaa gcttataata cttctggaaa
     1801 cgttggttat catgtatctt tcaatatgcg taattttcct aataagaaag tctatgtaat
     1861 gatgcgtgca acgaatgatc cagaagggaa cactaaaggc ggagcacaag attttcatga
     1921 taaacgctgg tatttaaata ttccgcaacg ataaaaaaag cccctcgat
//
LOCUS       DAFBYI010000207         1907 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFBYI010000207 DAFBYI010000000
VERSION     DAFBYI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.217"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="LLD04_002772"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="LLD04_002772"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK5649646.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="LLD04_002773"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="LLD04_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK5649647.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
BASE COUNT          669 a          311 c          412 g          515 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tattttt
//
LOCUS       DAFBYI010000208         1905 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFBYI010000208 DAFBYI010000000
VERSION     DAFBYI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.218"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..231)
                     /locus_tag="LLD04_002774"
     CDS             complement(<1..231)
                     /locus_tag="LLD04_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5649648.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYM"
     gene            complement(281..742)
                     /locus_tag="LLD04_002775"
     CDS             complement(281..742)
                     /locus_tag="LLD04_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5649649.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(724..1713)
                     /locus_tag="LLD04_002776"
     CDS             complement(724..1713)
                     /locus_tag="LLD04_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5649650.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          622 a          348 c          247 g          688 t
ORIGIN      
        1 catgtaatta tcatttgggg atgtttccat aatcccttta acatcactaa ttttaacaat
       61 gtctaatttg caatcagcag gtttagcaat tgtcaaagcc atcacagaca ttggatctcc
      121 tggaactttg ttatcagcca caataatatg gttaatatca tactgtttag tccaagaaaa
      181 tattacttgt ccatgaagta atctgtggtc tacacgcact aatttaatca ttctatttcc
      241 tccttcaatt tttaaaattt atagaataga aattgccacc ttaactgaca tcttttaaaa
      301 gcgaattaat atgaaccata cttttttgag agttattcag taattcctta aaatctattt
      361 cttctataga tttaccagct gactgggcca ttaataactc cagtaaaaga ggtaaattaa
      421 tcccagcaat aatttgaatt ctattgttgt tcaactgttt aatagaaaca ttattaggtg
      481 ttcctccaat taaatccgtt aaaataatta gaggttgcaa atgatgctga ctaattattt
      541 ctttcatact ctcctcaaaa tcatctggac tagtattttc tttaaatgga acagcactaa
      601 tacctgtggc gtcattcatt aaaattaatt ttgttgcctt taaataaccc tcacaaaggg
      661 tagaatgtgt tgtaatcaca atgtgaaata attgatttgc catcttttgc aacctcctat
      721 taattaatat ttcatttgcc acatatagcg tctaacatct aaggaatggc ctctttcaaa
      781 agcaatcgct tctaccattt ggcgaataac aacgccacta agtacagctg caatatattc
      841 tttcgctaca ctatctatag attctaagtc aaaatcagct tggtctaaaa ccattaattt
      901 attactgaat tttttagtga agtcttctac ccgttgatct aaaaatcttg tatctccaat
      961 acttttcact aagatgaatg gtacatcata atcagtgatt tcaaaaggac catggaaaaa
     1021 ttccccagaa tgaatagcat tagaattaat ccattgcatt tccatgaacc agcaagctgc
     1081 tgtggagtaa atttctccgt aattaatacc gctaccaatt gtgtaaataa ttttatctcg
     1141 tttttggttt ttaccccatt ctttagcagt tatttcaaat ttgctctttt cagcaataac
     1201 aagatcattt aacttatcaa attcattcaa gcaagtttcc cattttttag agccatctaa
     1261 aacaagtaaa atattaaata ataaaccata aagaattcct ttatttaaat cactagcatc
     1321 aacatcttta ccccaatcat agtgaacagg atattcagct gcttcccata gaggagatcc
     1381 ttcttcatta gataaacaaa tagtcattgc tccaaattct cttgcttttt ttgttgcttc
     1441 tacagtttct ggtgttgtgc cagaatggga acaagtgata actaaagaat tttcagataa
     1501 tccttttggt atagcatagt taaattcgtt agcagtataa acatgacaag gtacagttaa
     1561 ttctttatca aatagatatt gaccagccat taaaacggct tgtgaacccc cacatgctgt
     1621 aaaatataca tgatcaattg tcttttcctt taacgctgcc attgctgttt caatctgttc
     1681 tttgtgtagt tgtctcattt ctttatccct cactctaata ggtattataa agttatataa
     1741 ctttacgata cctattatct atgaaattgc tttattttgc aatactattt ttcaaaaaaa
     1801 ttttttataa caattttata taataaaatg ccagactgta gacaaagtcc attttatgga
     1861 tgatgtctac agtcttttct tttataatag atttatgaaa acgaa
//
LOCUS       DAFBYI010000209         1873 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFBYI010000209 DAFBYI010000000
VERSION     DAFBYI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.219"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(315..1607)
                     /locus_tag="LLD04_002777"
     CDS             complement(315..1607)
                     /locus_tag="LLD04_002777"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK5649651.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          540 a          391 c          335 g          607 t
ORIGIN      
        1 tggagaggtt gtgaaaaagc tgctctgcat caagcagtaa gaacaatcag ataaaaatag
       61 cactccatat ttttcatctg attgggctta gtgccgtaga tgcaagcttt tgaacaccgt
      121 ttgttcggaa aaagggactg tgacacggtt tttgtcacag tcccttattc tgaataaaca
      181 gtgttcaaga gacagcctat cggcaataag gctaaacgtc tctttcacta cctcttcaag
      241 ttattattat cgtatagata aacacttcga ctcgctctgg taagaacgtc ctgattcagt
      301 cgtcgttttt atccttagat ttgcgcccag acgctttcta agatgtttgt ttgtgttcta
      361 tcaggtccga cagaaaatgt tgagatacgt acaccaacaa gctctgagat acgacgtaca
      421 tagttacgtg cgttttctgg cagttctgca agtgttttgc atcctgtaat gtcttctgac
      481 cagcctggca attcttcata gattggtttg cagcggttca actctttcaa gcttgctgga
      541 tagtggtaga ttagttcgcc atctaattca taagccgtac aaatttttac tgtttccaat
      601 ccacttaata catcgatcga gtttaaagat aggttggtaa tacctgaaac tcgtttagaa
      661 tggcgcataa ctacagaatc aaaccagcct acacgacgcg gacgaccagt tgtcgtaccg
      721 tattctcgtc ctacttcgcg gatttgttct cctgtttcat caaataattc agttggaaat
      781 ggtccatcac cgacacgtga cgtataggct ttgcagacgc cgacaacttt attgattttt
      841 gaaggcccta caccgcttcc gattgttacc ccaccggcta ctgggttaga agaagtaaca
      901 aaaggataag ttccctggtc gatatccagc attacacctt gtgcgccttc aaacaacacg
      961 cgtttgcctg catccaaagc atcattcaag ataacggaag tatctgtgac gtattgtttg
     1021 atttgttgtc cgtattcata atattcttca aaaatatcgt caaaatcaag tggttctgaa
     1081 tcaaacattt ttacaaattg acggtttttg tcatctagat tcaaacgaag acgttcttca
     1141 aaaatttctt tatctaataa gtctgcaata cggataccta cacgagctgc tttatccata
     1201 taggcaggtc cgattccttt aatcgttgta ccaatcttat tttcgccctt agcatcttct
     1261 tgtaattggt ccaatttgat atgataaggc aagattacgt gcgcgcggtc agaaatacgc
     1321 aaattacttg tttctacacc tttttcatgt aaataagcta attctttaac caatgattta
     1381 ggattgacta caacgccgtt tccgatcaca ctgatcttgt ctttataaaa aatcccagaa
     1441 ggaatcaaat gcagtttata agtcacaccg ttaaacttaa ttgtgtgccc tgcattgtcg
     1501 ccaccttggt accgtgcaat cacttcagca ttttcactga gaaagtctgt gatttttcct
     1561 ttcccttcat cgccccattg cgtacctact actactacag atgacatgtg aacacctcgt
     1621 tctatattat ttaatcctga accattgtat caataataga tacatatttc aaataaaatc
     1681 gaatgatttt tatttttatt ttataaaaac gcttaaaaaa cgaactttat ctttttagaa
     1741 tggttattaa aaaagaggtt gtaacagaag tggtcagctt caagaaataa gaaggaatgt
     1801 acgaaaattg cttctcaaat ttttgtgaaa tttcgactta tttttcgaag aagctacttt
     1861 tggaacaccg tga
//
LOCUS       DAFBYI010000210         1787 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFBYI010000210 DAFBYI010000000
VERSION     DAFBYI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.221"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..126)
                     /locus_tag="LLD04_002778"
     CDS             complement(<1..126)
                     /locus_tag="LLD04_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649652.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLD"
     gene            654..737
                     /locus_tag="LLD04_002779"
     tRNA            654..737
                     /locus_tag="LLD04_002779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Leu,seq:cag)
     gene            complement(1061..1699)
                     /locus_tag="LLD04_002780"
     CDS             complement(1061..1699)
                     /locus_tag="LLD04_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5649653.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          595 a          289 c          356 g          547 t
ORIGIN      
        1 atctaaaaat tcagcaaatg tatagccata agcattgttg ataaatccac tctcatttgg
       61 tccaaagcta gtttgtagct gataagcttt ctttaaatct gcttcccata gtttgatcag
      121 tttcatcgta ttcacctctt taagtaacct tattcatttg ttttctacta tagcactatt
      181 gttgtaaata gccatgccta tttttattta ttgtgtcata tcgtattaag aatcatcgaa
      241 caatacaaat atgaattaca ataaaaaaat tatttgaagt tcgttcattc gttacttatg
      301 atctctagaa gaaagaaaac gtgaattact tcagcgtcta gaattccaga aggatagtaa
      361 tcaacagaag aaataatcaa tatccaaatg aaagaagata gctgaatcga gaaaaagagg
      421 aaaaaagaaa tgaagaaaaa aataaaatag ttactaatcc cagagtcgtt cgtttaacat
      481 tgaaatcgat catatggata aaacagattt tttcaccaac cctcaataga tgatggagtt
      541 gctcggatga attatttgtg tagatggcat aagggagggc gtacagaaat gaaaaagaac
      601 aaaaatatgt tcttgacaaa aaacacatgt aatcctaaac taaaagatgt gatgcggcta
      661 tggcggaatt ggcagacgcg ctagcttcag gcgctagtga gggtgacctc gtggaagttc
      721 gagtcttctt agccgcataa tttgtttttt tataaagaga aggtacacct ggagcgatga
      781 gaaagaaaat aacatattat ctaaaagaat ataggaaaaa ttttaaacaa cggaaccaga
      841 ttcaaataaa tcaaaaaaaa tttgtgtgaa ggtgggacag tttaaaacct aatagattga
      901 ttcaaatagt agatttatag attctgatac aagaaatact ggcaaacgat agttaaggaa
      961 taaagactaa acttaaactc aaaaagaggt catgcgaaag aataaatcgc atgacctctt
     1021 actatatttt gaatgtgttt aaacgttcaa ataatgaatc ttagtaccaa ccgtttgctt
     1081 cccagaatgc tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta
     1141 ctctttcttg gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac
     1201 ggccgatgta acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc
     1261 attcttttgc agagcttgta cttgctggag tagcagcagt ttctgttaca gcaggagcag
     1321 gttgtgtttc agcaacaggc gctgcttgtg tttccgtttg aaccgtttct tgtacaacag
     1381 gttgttcagc ttgaacaggt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt
     1441 cagcagctgc ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagtagatca
     1501 ggttaatatc agaaattgaa tttgattctg caatggcatt gatcaaggag ttatcaccaa
     1561 cgtatttttg agaaatcgtt gatagagtat cacctgattg aacggtataa gcttcgtccg
     1621 catgagcagt cgttcccatg aagaatgcag cgccggcagc caaagttgtt ccaaaaagta
     1681 aagttttaag tgatgtcatg aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg
     1741 ttcaacgagg aatactttaa cacgtgaaaa tatttcatag gtaaaag
//
LOCUS       DAFBYI010000211         1709 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFBYI010000211 DAFBYI010000000
VERSION     DAFBYI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.222"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(62..280)
                     /locus_tag="LLD04_002781"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="LLD04_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="LLD04_002782"
     CDS             complement(546..740)
                     /locus_tag="LLD04_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649654.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="LLD04_002783"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="LLD04_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          599 a          250 c          227 g          633 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaag
//
LOCUS       DAFBYI010000212         1701 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFBYI010000212 DAFBYI010000000
VERSION     DAFBYI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.223"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..255
                     /locus_tag="LLD04_002784"
     CDS             <1..255
                     /locus_tag="LLD04_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649655.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="LLD04_002785"
                     /pseudo
     CDS             258..411
                     /locus_tag="LLD04_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="LLD04_002786"
     CDS             434..1639
                     /locus_tag="LLD04_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5649656.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DAFBYI010000213         1700 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFBYI010000213 DAFBYI010000000
VERSION     DAFBYI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.224"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            466..1065
                     /locus_tag="LLD04_002787"
     CDS             466..1065
                     /locus_tag="LLD04_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5649657.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="LLD04_002788"
     CDS             1129..>1700
                     /locus_tag="LLD04_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBK5649658.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DAFBYI010000214         1663 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFBYI010000214 DAFBYI010000000
VERSION     DAFBYI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.225"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="LLD04_002789"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="LLD04_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK5649659.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            975..1663
                     /locus_tag="LLD04_002790"
                     /pseudo
     CDS             975..1663
                     /locus_tag="LLD04_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          517 a          324 c          307 g          515 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1081 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1141 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1201 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1261 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1321 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1381 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1441 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1501 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1561 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1621 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaa
//
LOCUS       DAFBYI010000215         1642 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFBYI010000215 DAFBYI010000000
VERSION     DAFBYI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.226"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(1..>1642)
                     /locus_tag="LLD04_002791"
     rRNA            complement(1..>1642)
                     /locus_tag="LLD04_002791"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          348 a          507 c          350 g          437 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcaa ttcgtacctt
     1021 cgcttgcgct aagcactcct cttaaccttc cagcaccggg caggcgtcag cccctatact
     1081 tcatcttacg attttgcaga gacctgtgtt tttgataaac agtcgcttgg gcctattcac
     1141 tgcggctgat cgtatgatca gcaccccttc tcccgaagtt acggggtcat tttgccgagt
     1201 tccttaacga gagttctctc gctcacctta ggattctctc ctcgactacc tgtgtcggtt
     1261 tgcggtacgg gccgctgttt tctcactaga agcttttctc ggcagtgtga catcaggaac
     1321 ttcggtacta ttatttccct ccccatcaca gcttgtcctt aagaaaagaa gcatttgact
     1381 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
     1441 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
     1501 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
     1561 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
     1621 ttctcacttc taagcgctcc ag
//
LOCUS       DAFBYI010000216         1629 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFBYI010000216 DAFBYI010000000
VERSION     DAFBYI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.227"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            287..694
                     /locus_tag="LLD04_002792"
     CDS             287..694
                     /locus_tag="LLD04_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649660.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LLD04_002793"
     CDS             708..1109
                     /locus_tag="LLD04_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649661.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LLD04_002794"
     CDS             1111..1482
                     /locus_tag="LLD04_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649662.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          525 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtataca
//
LOCUS       DAFBYI010000217         1538 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFBYI010000217 DAFBYI010000000
VERSION     DAFBYI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.228"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            87..1523
                     /locus_tag="LLD04_002795"
     CDS             87..1523
                     /locus_tag="LLD04_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5649663.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT          521 a          252 c          356 g          409 t
ORIGIN      
        1 tttacataat tttattttgt tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt
       61 gaaggttata ggaaaggaag agatgaatgg aacacaaaga acttacctca tttccaaaag
      121 attttctgtg gggctctgct tctgcagcgt atcaagtaga aggagcttgg caagaagatg
      181 ggaaaggaga atcagtctgg gatcgatttg tacggatacc tggaaagaca tttaaaggaa
      241 cgaacggtga tttggcagta gaccactatc atcgttataa agaagatatt gctttaatga
      301 aagaacaagg tctgaaagct taccgcttct ctgtagcttg gacacggatt tttcctaatg
      361 gacgaggaga agtcaatcaa gctgggctcg ctttttatga gagattgatc gacgaattga
      421 ttgaaaacga tattgaacct atgttgactt tatatcattg ggatcttcca caagcattgc
      481 aagatgaata taatggttgg gaatcacgtc agatcattga agatttcact cattatgcag
      541 aaacattatt tgaggcattc cgcgggaaag tccattactg gattagtttg aatgaacaaa
      601 atatcttcac ttcattaggt tatttgttag cagcacatcc gccaggtgtg acagacccga
      661 aacgaatgta tgaagttaac catattgcta atttagcaaa tgcatctgtc atcaataaat
      721 ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc
      781 caattgacag taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca
      841 attattggat ggatgtctat atgtggggaa aatatccgat tgctgccatg aggttcttag
      901 aagaaaaagg atgggcacca acgattgaag ctggcgatgc agaattgcta gaatctgcta
      961 aaccagattt tcttgggatc aattactatc aaacggcgac gaatgcattc aatccattaa
     1021 acggagttgg tgctggaaaa atgaatacga caggaaaaaa aggttcatca gaagaaactg
     1081 gagttcctgg aatgtataaa aaagttgaga atccatttgt cgaacgaaca aattgggact
     1141 gggagattga cccagaagga ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca
     1201 tgccggtact gattacggaa aatggcttag gagagtatga caagttgaca gaagataaac
     1261 aaatccatga cgactatcga atcaattact tacaaagcca cgtcaaagcc atcaaagaag
     1321 caatttcgga tggagcagaa gtcctaggct attgcacctg gtcttataca gatttgctta
     1381 gctggctgaa cggttatcaa aagcgatatg gatttgtata tgttgatcaa gacgaaacac
     1441 aagaaggatc tttggaacga tataagaaag acagttatta ctggtaccaa aaactgattg
     1501 aagaaaatgg agagaacctt tagttcacat acttaaaa
//
LOCUS       DAFBYI010000218         1534 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFBYI010000218 DAFBYI010000000
VERSION     DAFBYI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.229"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            419..973
                     /locus_tag="LLD04_002796"
     CDS             419..973
                     /locus_tag="LLD04_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5649664.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1227..>1534
                     /locus_tag="LLD04_002797"
     CDS             1227..>1534
                     /locus_tag="LLD04_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649665.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          615 a          159 c          260 g          500 t
ORIGIN      
        1 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
       61 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
      121 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
      181 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
      241 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
      301 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
      361 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
      421 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
      481 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
      541 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
      601 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
      661 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
      721 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
      781 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
      841 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
      901 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
      961 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     1021 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     1081 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     1141 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     1201 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     1261 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     1321 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     1381 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     1441 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     1501 aaatttttgc cagcattcat gattatatag gttt
//
LOCUS       DAFBYI010000219         1453 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFBYI010000219 DAFBYI010000000
VERSION     DAFBYI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.230"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            105..1037
                     /locus_tag="LLD04_002798"
     CDS             105..1037
                     /locus_tag="LLD04_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5649666.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1109..>1453
                     /locus_tag="LLD04_002799"
     CDS             1109..>1453
                     /locus_tag="LLD04_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649667.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          639 a          169 c          226 g          419 t
ORIGIN      
        1 aaataaatca aaagtatgat gaaacattaa aaaatgtcta ttcttcactt atcgtttcaa
       61 atatagaaga aacttcatct tctgaaataa aaaataagct ttctttgtta aaattaaaag
      121 aaaaggaata tattattgac gatgctgttt ctgtccctct tattttaaat aaacgagaga
      181 aaaataattt aaagaaaaaa atgctcattc catttgattc agaagtgaaa ggattactca
      241 ataaactaac tatttctaat atagactcga ctagggaaaa aatcataaaa atatttgata
      301 aaattaataa tttattcaag aatgacaatg ctgaattaag gaagggtctt ttagagttta
      361 aacttcaaga actagaactt cattactctt atttagtgaa aattaccgaa gaaaaagaac
      421 aacaaaaagc aataaaagaa cagatgatag aagaggaaaa agttcgtaga gaaattgatc
      481 gtgaaaagaa acgtattgat aaagaacaac gtcaattcaa ttctgaaatt tccaaattaa
      541 tcgcatatat gcaaaaagct aatgcagatg tggaaaaaga attatatgca aataaaattc
      601 aagaactgga agaaaaatta aaagaattag aagttatacg tgaaaatgta ttacaaagag
      661 aattaaatac tagagccggt tatgtgtatg taattagtaa tattgggtct tttggtgaag
      721 acatatataa aattggtatg actagaagat tagaacctat ggatagaata aaagaattga
      781 gtagtgcatc ggttccattt gaatttgacg ttcatgcgat gattttttca gaggacgctc
      841 cgtctttgga aactaaattg cataatcatt ttagaaaaca agaagttaat aaaattaatc
      901 aaagaaaaga atttttcaaa gtttcactag acgaaataga gaaagttgtt ttagaaaatt
      961 ataatggaac agtaacattt acaaaattag ctaaagctga acaatataga cgatctttag
     1021 aattgtcaaa agattaaata tataaaaagc cttcgggctt tttcttttaa caattaatag
     1081 gaacgtatat tcgaaaggaa tagaaataat gtggttagaa aaaataaatg acaaaaaatt
     1141 taagtacacc gaacgttata cggatccctt aactgaaaaa aagagaaaag tgtccgttac
     1201 tttatctagc aattcacgcc aagcttggaa tcaagctaac ttacttttga atgaaaaaat
     1261 cgcagaaaaa ataaaacgaa atgaagaatt accactaact tttggagaat taaaaactaa
     1321 atgggatgaa aaatacaagc caactgtaaa agaaagttcc tatcgaacaa ctcaggttta
     1381 tctatcatta atttcaaaat atataaaaga tgatgtactt gtaaaaaacg ttaatagtaa
     1441 tcttatacaa gat
//
LOCUS       DAFBYI010000220         1425 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFBYI010000220 DAFBYI010000000
VERSION     DAFBYI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.231"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..226
                     /locus_tag="LLD04_002800"
     CDS             <1..226
                     /locus_tag="LLD04_002800"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK5649668.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="LLD04_002801"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="LLD04_002801"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="LLD04_002802"
     CDS             533..1336
                     /locus_tag="LLD04_002802"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK5649669.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          453 a          256 c          337 g          379 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttatt
//
LOCUS       DAFBYI010000221         1424 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFBYI010000221 DAFBYI010000000
VERSION     DAFBYI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.232"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(150..1337)
                     /locus_tag="LLD04_002803"
     CDS             complement(150..1337)
                     /locus_tag="LLD04_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK5649670.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFBYI010000222         1358 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFBYI010000222 DAFBYI010000000
VERSION     DAFBYI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.233"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(202..690)
                     /locus_tag="LLD04_002804"
     CDS             complement(202..690)
                     /locus_tag="LLD04_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649671.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(712..1314)
                     /locus_tag="LLD04_002805"
     CDS             complement(712..1314)
                     /locus_tag="LLD04_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5649672.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          422 a          318 c          196 g          422 t
ORIGIN      
        1 cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt ctccacaaaa
       61 gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat tatcctgtgc
      121 ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc gcataaattt
      181 actttaacac agttcctatg tttatgcgag agcttttttc attttataat cgaccatttg
      241 aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt catgcttata
      301 aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc tttcctttgt
      361 ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt gaccacgtac
      421 tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt cataagcagt
      481 gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt ctgtcagttc
      541 tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt gtttttgacc
      601 aatgatcggc acaaaagctt ccggccatac ttcttgtatg actgccatca gctcatccag
      661 ttcattttct gttactttta ttttttccat acacccacgt ccttttttac attataaatg
      721 accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca ccgaccacgg
      781 tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca aaccagctac
      841 tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta gtactttttt
      901 aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt ttggcgataa
      961 aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga ttgcttcccc
     1021 aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca tcagcatgcc
     1081 cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca cttgcaacag
     1141 aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca aagacgtgct
     1201 cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa aaaacagacc
     1261 aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag acatctatat
     1321 tccccacatc ttttttcatt tattaaaact gcctcctt
//
LOCUS       DAFBYI010000223         1352 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFBYI010000223 DAFBYI010000000
VERSION     DAFBYI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.234"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            11..1350
                     /locus_tag="LLD04_002806"
     CDS             join(11..452,452..1350)
                     /locus_tag="LLD04_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK5649673.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DAFBYI010000224         1343 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFBYI010000224 DAFBYI010000000
VERSION     DAFBYI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.235"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            44..1339
                     /locus_tag="LLD04_002807"
     CDS             44..1339
                     /locus_tag="LLD04_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5649674.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          420 a          267 c          257 g          399 t
ORIGIN      
        1 cctttagaat taatttggct accaattcat aggaggattc aagatgtctc atactcagct
       61 tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac
      121 taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc
      181 agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac
      241 aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa
      301 acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga
      361 aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa
      421 tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt
      481 catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg
      541 ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga
      601 cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg
      661 ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat
      721 gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga
      781 tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac
      841 cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg
      901 ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg
      961 tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg
     1021 tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag
     1081 agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta
     1141 tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta
     1201 cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa
     1261 tgcgtacggc tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa
     1321 aacattttta accaaataaa cag
//
LOCUS       DAFBYI010000225         1336 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFBYI010000225 DAFBYI010000000
VERSION     DAFBYI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.236"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(146..1129)
                     /locus_tag="LLD04_002808"
     CDS             complement(146..1129)
                     /locus_tag="LLD04_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK5649675.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DAFBYI010000226         1324 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFBYI010000226 DAFBYI010000000
VERSION     DAFBYI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.237"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            55..1217
                     /locus_tag="LLD04_002809"
     CDS             join(55..331,331..1217)
                     /locus_tag="LLD04_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5649676.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          478 a          234 c          265 g          347 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acaccggttg
     1141 gttaccgtaa ccagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgctaacg
     1201 ctagcgaggc agtctaactt taactgttag ctcctgccga agatcgtcac atccgaggag
     1261 gctcattgtc aaggacaatc ggaatagcat acggaaagaa gttctgcacc ttataaaatt
     1321 tgtc
//
LOCUS       DAFBYI010000227         1323 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFBYI010000227 DAFBYI010000000
VERSION     DAFBYI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.238"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..574
                     /locus_tag="LLD04_002810"
     CDS             <1..574
                     /locus_tag="LLD04_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649677.1"
                     /translation="LDIAKRNTAFLLYRVLLRAGLITFISIVGFFLFTVIVFIWRTTA
                     PPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRGNVTTQPRRSWSIKLLNNS
                     GELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQLTVRDRIAGFMHFSHYDLE
                     EARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            761..>1323
                     /locus_tag="LLD04_002811"
     CDS             761..>1323
                     /locus_tag="LLD04_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK5649678.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          430 a          161 c          264 g          468 t
ORIGIN      
        1 tctagatata gcaaagcgaa atacagcatt tttattatat cgggttttac ttcgagcagg
       61 tctcataaca tttatatcga tcgttggatt ttttctattc acagttattg tatttatttg
      121 gagaactaca gcaccaccat tgccagagat tacatatgga gaatttccgt ttagtcttga
      181 atatgaaata gatgaggaaa gaattttgat tgaagatacg gtgattgcag aatttatagg
      241 aagttttaga gggaatgtga caactcaacc tagacgaagt tggagtataa aacttttaaa
      301 taatagtgga gagttgaaag catttcatta tgaattctta ataatagaaa gtgaagatgt
      361 aacaatcact ttaagtagtg gctctgctgg atattttatg aatgatggtg gaagcaagcc
      421 acagctcaca gtccgtgatc gtattgcagg ttttatgcat ttttctcact atgacttaga
      481 agaagctcgt aaaatacttg cagaatatgg gatcacatta atcaattggg aatatgcgcc
      541 accgataaga aattttttca atgattatta ttagtcttat aaaaaacaga atgtaaaatt
      601 tatgtgcgcg agtatttagt tttccatttt ttgtttgata gatatatagc attgttgaag
      661 catgggatag tggtgccaat tttatcaatg aggttggtca ggcagttaac ataattggtg
      721 atgctataaa tccatttaat tggaattgga ggaattaggt gtggaaacag tgagattatg
      781 gatattcatg aaaaattttc gttcggcttt gagatatgtt attctggaat caaaatcagg
      841 ttattatctg attgacacag atcactatac tttttgggga ggctaccttt ttccgatttt
      901 taattggata atcccccata aggttatgaa aattaacttg cctgcagaag aaataaatag
      961 attaatactc aacaaagatt tacaaaggaa aagagagaaa gaacattcta atgttgcttg
     1021 gataggtcca gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc tagagagtat
     1081 ttatagtgat ttttctattg gaatacgtta tttatttgtt actttaagta tagctgttct
     1141 tttgtgcata aagggttatg ttagcaagaa gaataaagat attttaacac taatcggtgt
     1201 aaacaattta tctagtacta aaattatact gtttcctgaa tctataaaag aaggatcaag
     1261 gttgttgata atttttataa tgcttatact gttttctgtt ttatttgggg tgatgtttat
     1321 aga
//
LOCUS       DAFBYI010000228         1319 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFBYI010000228 DAFBYI010000000
VERSION     DAFBYI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.239"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1285
                     /locus_tag="LLD04_002812"
     CDS             <1..1285
                     /locus_tag="LLD04_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5649679.1"
                     /translation="IKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEIRRCPLC
                     KQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTYIVDRNC
                     FIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWLPRCLLM
                     DEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKVKVIVTD
                     MNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQAKYYRQL
                     KALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQ
                     AFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTH
                     IKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          429 a          261 c          245 g          384 t
ORIGIN      
        1 tatcaaacta ctacttggaa tcaaagataa aaacattttc atcacaaatg tggaatcgaa
       61 gagcattaag aatatcaaga gtcttgttgt ttctgcgacg ttaagtaaag aaattcgtcg
      121 ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc gttaagaacg gaaagaaaaa
      181 atcgctcatt caacttaata aatgtgcgaa ccaactgact tatcttgctt tagcgaaaca
      241 acgctaccac tgtcgtggct gccacaccta ttttacggcg aatacctaca ttgtcgatag
      301 aaactgcttt atcgctaaac aagtgcgcta taaaatttta gaagagctaa ctgaaaaaca
      361 agcaatgacg acaattgcta agcattgcgg cgtttcttgg tcgaccgttt ctcggacact
      421 cgcttctctg atccccatga caaaagtaaa aagaaattgg ttaccgcgtt gtttattaat
      481 ggatgaattt cgttcactga aaaatcaagt cggtccctac tccttttctt gtatggatgg
      541 cgatactgga aaattactgg atattcttcc ttcacggaag aaaaaggctc tcgtttctta
      601 ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag gtcatcgtga cggacatgaa
      661 tgtttcttat gcctctctga taaaggaatg ttttccaaag gcaaaactcg ttgtggatcg
      721 ttttcacatt gtcaaacatc tcattcgtaa atttgaggat attcgcgtac gaatcatgaa
      781 aagctttgat cgaaacgatc caacacaagc caaatattat cgacaattaa aggcgttgtc
      841 tcggcttctg atcaaacgac aggacaagct tgtttatgac aaatggacca aatggcgcaa
      901 ttttggttgg gcgtatctca ccgaaagtga agtggtggat cgtctgcttt cgatctctga
      961 cgaattacga atcgcctatt tttactatca agaaattctt caagcgtttc acgacaaaga
     1021 ggcggacact ttctttaaac ttgtaagaac gatgcccaat tccgttccaa aagaactgca
     1081 ttctataaaa aaagctttta taaactatga atccggcatt cgtttagccc tcgaattgcc
     1141 ttattcaaat gccaaaatag aaaatctgca tacccacatc aaagcattga aacgagtcgc
     1201 ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt ttcttactaa ataatctgat
     1261 tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt ctaccttaga tgttcttat
//
LOCUS       DAFBYI010000229         1311 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFBYI010000229 DAFBYI010000000
VERSION     DAFBYI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.240"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            200..742
                     /locus_tag="LLD04_002813"
     CDS             200..742
                     /locus_tag="LLD04_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649680.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="LLD04_002814"
                     /pseudo
     CDS             898..1248
                     /locus_tag="LLD04_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAFBYI010000230         1298 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFBYI010000230 DAFBYI010000000
VERSION     DAFBYI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.241"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            82..>1298
                     /locus_tag="LLD04_002815"
     CDS             82..>1298
                     /locus_tag="LLD04_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK5649681.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKR"
BASE COUNT          440 a          254 c          248 g          356 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacg
//
LOCUS       DAFBYI010000231         1293 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFBYI010000231 DAFBYI010000000
VERSION     DAFBYI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.242"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..168
                     /locus_tag="LLD04_002816"
     CDS             <1..168
                     /locus_tag="LLD04_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5649682.1"
                     /translation="EIEVTDILQDDLFSDNHEVEKVGTVEFPIEEGTLIIATVDDPKQ
                     NVFTLEKVGVI"
     gene            168..956
                     /locus_tag="LLD04_002817"
     CDS             168..956
                     /locus_tag="LLD04_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649683.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKVGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATV
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NADVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVTQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            961..>1293
                     /locus_tag="LLD04_002818"
     CDS             961..>1293
                     /locus_tag="LLD04_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649684.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTTASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGL"
BASE COUNT          446 a          211 c          311 g          325 t
ORIGIN      
        1 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca cgaagtcgaa
       61 aaagttggaa ctgtagaatt cccgattgaa gaaggaacct tgatcatcgc aaccgttgat
      121 gatccaaagc aaaatgtatt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      181 aacgaatgat ggaaacggat gaaaaaacag gtgtacaacg ccagttcttt ccaatcacac
      241 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      301 ttaatggaaa agttggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
      361 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
      421 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact gtcgaccgtt
      481 tgggtggcat aaaaattggt gaattattga cgattgatga gacagggaaa gtctctgcag
      541 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
      601 attatactgc tggagaaaac atcactattg atgaagatgg aaaaataaat gcagatgtga
      661 ctggttttta cgttttaccc actgcctccg aatttgtaaa aggtggtatt cgtgtcggtg
      721 aaggattaac gatgaccgat gatgtgttgt ctgccgataa gcaattcaac tatactgccg
      781 gagctaatat cagtatttca aacacaggag taatctctgc aacaggtggt ggagaaggtg
      841 gcggcgtcac tcaagaatat gttgatcaaa aaatgagtga agcttatcaa aatgcacaag
      901 cttatacaga ttccaaaata ccaaatgtat cgtttgaaaa agtaggggag gtatagacaa
      961 atgacagata ttgtaaaagt aaaacagaat gatgttcagg tttaccctca aacccattgg
     1021 gatgctgttg agggtaaacc cgaaactatc aagggagata aaggagaccc tggacaggct
     1081 gccacaatta ctgtagggac agtaacgagt ggtacaacag ctagtgtcac aaatgctggt
     1141 actgcaagtg cagcaaagtt taattttgta ttgccaaaag gtgataaagg ggacaaaggc
     1201 gatcctggag ccaatgcaac aacgacagct gtggctacga cgacagcaaa tggtttgatg
     1261 tccaaagaag ataaagtgaa actcgatgga tta
//
LOCUS       DAFBYI010000232         1287 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFBYI010000232 DAFBYI010000000
VERSION     DAFBYI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.244"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            316..570
                     /locus_tag="LLD04_002819"
     CDS             316..570
                     /locus_tag="LLD04_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5649685.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="LLD04_002820"
     CDS             571..888
                     /locus_tag="LLD04_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5649686.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          449 a          208 c          255 g          375 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatg
//
LOCUS       DAFBYI010000233         1276 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFBYI010000233 DAFBYI010000000
VERSION     DAFBYI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.245"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            35..532
                     /locus_tag="LLD04_002821"
     CDS             35..532
                     /locus_tag="LLD04_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5649687.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="LLD04_002822"
     CDS             complement(569..1252)
                     /locus_tag="LLD04_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649688.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAFBYI010000234         1270 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFBYI010000234 DAFBYI010000000
VERSION     DAFBYI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.246"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..132
                     /locus_tag="LLD04_002823"
     CDS             <1..132
                     /locus_tag="LLD04_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5649689.1"
                     /translation="IRSLQREIDDLERTKAVMVNETARKAIDLHIENLRREIRRLEE"
     gene            135..440
                     /locus_tag="LLD04_002824"
     CDS             135..440
                     /locus_tag="LLD04_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649690.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVKKIGKSMWAEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWTDSKGRQFRYVEEKKC"
     gene            543..941
                     /locus_tag="LLD04_002825"
     CDS             543..941
                     /locus_tag="LLD04_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBK5649691.1"
                     /translation="MIPKFRAWKTIGTNEMFHNVVAISWNNNKIAVLYPNPGRNTFRI
                     DEESLENVILMQSTALKDKNGVDVYQGDIIRCASGCPHEVIWLEEYGGTFFGGMPAWY
                     LSGLSNGYAWTGREEVIGNIYENSELLEVE"
     gene            938..>1270
                     /locus_tag="LLD04_002826"
     CDS             938..>1270
                     /locus_tag="LLD04_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649692.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEA"
BASE COUNT          457 a          185 c          313 g          315 t
ORIGIN      
        1 attcgaagcc ttcagcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat
       61 gaaacggcga gaaaggcaat cgatttgcac atagagaatt taagaaggga aattcgtaga
      121 ttggaggaat aaacgtggat aagaaagcag caatgaaacg aattgctgaa ttaaccaagt
      181 cagaatcttg gcaggaagac aaagaaatag ttgcagaagt caaaaagatc ggcaaatcaa
      241 tgtgggctga aaaaaccaaa cggagaacac cgagaaagat tgcaatctgg catggtgatc
      301 gaattctagt aacaggtacc gctgaacagt tatctgaaat tactggtctg agcaaaaaca
      361 ttatttggga tagagctagg agcttgtgga ctgattcaaa aggacgacag tttaggtatg
      421 tggaggagaa aaaatgctag acatgaaaat cgaagattat cgaattacca gtgattccag
      481 gaacattgtc ctatcaaaag taagacgaga cggagtaaga gagcttacga ggaggacagc
      541 aaatgatacc gaagtttaga gcatggaaaa caattggtac aaatgaaatg tttcataacg
      601 tagttgctat aagttggaac aataataaaa tagctgtttt atatcctaat cccggaagaa
      661 atacattccg aattgacgaa gaaagtctgg aaaacgtgat tctcatgcag tccacagctc
      721 tgaaagataa gaacggtgtg gatgtctacc aaggcgatat catcagatgc gctagcggtt
      781 gtcctcatga agtaatttgg ttagaggaat atggaggtac attttttgga ggaatgccgg
      841 cgtggtatct ttcaggatta agtaatggct atgcgtggac tggtagagaa gaagtcatcg
      901 gaaatatata cgagaatagt gagttattgg aggtagagtg attatcttag ctactgacta
      961 catcgattct ttaaaagatg aaaatgggaa tgtcccagca ttgctaaatt gtggaattaa
     1021 aagcaagaat agaatcattt tgttacggat atcggctgat ttagcaaaat atctttatct
     1081 gagcaagaca gcagtatatt acactgcggc tacacgttat cagtacaagg gaaaaacaat
     1141 cagtcaggat tattatgatc gtttaatcag tcctgacatg catggaaaat caaaatcagc
     1201 gatcaagtta tttggcgcaa tagagatata tgccgatgat tttccaaatt tatggttgaa
     1261 ggaggaagcg
//
LOCUS       DAFBYI010000235         1267 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFBYI010000235 DAFBYI010000000
VERSION     DAFBYI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.247"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            66..1244
                     /locus_tag="LLD04_002827"
     CDS             66..1244
                     /locus_tag="LLD04_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HBK5649693.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          427 a          254 c          246 g          340 t
ORIGIN      
        1 aaaaaggaag tcccttctgt agaatagagt taccacaaca cattcacaga aaagaggact
       61 tccatatgaa cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga
      121 atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa
      181 cggctttcct cgattacgaa aagtacgatc gcattggttt taacacgggt aattctcgta
      241 acggctccta tgaccgtacg gtcaagaccg agtacgggga acttcatctc cagattccgc
      301 gcgaccgcaa cggcgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgata
      361 ccttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      421 acttgattga gaaaatgtat gggcatcact acacgcccca aaccatgtcc aacatgacta
      481 aagtgctgac tgaagaagta aatgcattta aagttagaac tctaaatgat aagtatgtcg
      541 ctattttcat ggatgctact tatattcctt taaaacggca aaccgtctcc aaagaagcaa
      601 tctatattgc cattggtata cgagaagacg gcactaaaga agtactaagt tatgcgattg
      661 ccccgactga atcgacctac gtttggaatg agttgttaca ggacatttac tccagagggg
      721 ttcatgatgt cttactgttt attaccgatg gcttaaaagg catgaaagat acgattcacc
      781 agatttatcc taaagctaaa taccaacatt gctgtgttca cgtttcccgt aatattgctc
      841 ataaagtacg tgtcaaagat cgaaaagaaa tctgtgatga ttttaaggct gtttatcaag
      901 ctagctcaaa ggaagaggca aatacctttt tagggagtat gattgagaag tggcagaaaa
      961 cttatcctaa agtgacgcag tcactgataa aaaatcaaga tttattgact ttttatgagt
     1021 ttccgcctgg tatccgccga agcatttact caactaattt aatcgaatct ttcaataaac
     1081 aaatcaagaa atacagccgc agaaaagagc agtttcaaaa tgaagaatca ctagagcgtt
     1141 tcctagtatc catctttgat acatacaatc aaaaattctt aaatagaagc cataaaggct
     1201 tccaacaagt gacggataca ttagcttcaa tgtttactga gtaacccatt attttgcaga
     1261 aggacga
//
LOCUS       DAFBYI010000236         1233 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFBYI010000236 DAFBYI010000000
VERSION     DAFBYI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.248"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            98..>1233
                     /locus_tag="LLD04_002828"
     CDS             98..>1233
                     /locus_tag="LLD04_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5649694.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQ"
BASE COUNT          395 a          255 c          228 g          355 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aat
//
LOCUS       DAFBYI010000237         1215 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFBYI010000237 DAFBYI010000000
VERSION     DAFBYI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.249"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            2..>1215
                     /locus_tag="LLD04_002829"
     rRNA            2..>1215
                     /locus_tag="LLD04_002829"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          368 a          242 c          352 g          253 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctaggatgtt ggcttagaag cagccaccat ttaaagagtg cgtaatagct cactagtcga
     1141 gtgaccctgc gccgaaaatg taccggggct aaacatatta ccgaagctgt ggagtacacc
     1201 tttaggtgta ttggt
//
LOCUS       DAFBYI010000238         1215 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFBYI010000238 DAFBYI010000000
VERSION     DAFBYI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.250"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            414..947
                     /locus_tag="LLD04_002830"
     CDS             414..947
                     /locus_tag="LLD04_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK5649695.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAFBYI010000239         1202 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFBYI010000239 DAFBYI010000000
VERSION     DAFBYI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.251"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..168
                     /locus_tag="LLD04_002831"
     CDS             <1..168
                     /locus_tag="LLD04_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5649696.1"
                     /translation="EIEVTDILQDDLFSDNHEVEKVGTVEFPIEEGALIIATVDDPKQ
                     NVFTLEKVGVI"
     gene            168..785
                     /locus_tag="LLD04_002832"
     CDS             168..785
                     /locus_tag="LLD04_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649697.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            789..>1202
                     /locus_tag="LLD04_002833"
     CDS             789..>1202
                     /locus_tag="LLD04_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649698.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGL"
BASE COUNT          435 a          177 c          266 g          324 t
ORIGIN      
        1 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca cgaagtcgaa
       61 aaagttggaa ctgtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      121 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      181 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      241 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      301 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
      361 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
      421 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
      481 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
      541 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
      601 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
      661 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
      721 aagcagaaag ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
      781 tatagaaaat gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa
      841 ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg
      901 gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga
      961 atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg
     1021 atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt
     1081 cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta
     1141 atgctacaac tacgacagca ggtttaatgt cagcaaccga taaagtgaaa ctcgatggat
     1201 ta
//
LOCUS       DAFBYI010000240         1193 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFBYI010000240 DAFBYI010000000
VERSION     DAFBYI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.252"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..1076
                     /locus_tag="LLD04_002834"
     CDS             <1..1076
                     /locus_tag="LLD04_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5649699.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFBYI010000241         1192 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFBYI010000241 DAFBYI010000000
VERSION     DAFBYI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.253"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..919)
                     /locus_tag="LLD04_002835"
     CDS             complement(<1..919)
                     /locus_tag="LLD04_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649700.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPE"
BASE COUNT          282 a          214 c          226 g          470 t
ORIGIN      
        1 attcaggtgt tgttgattct tctgttgctg aagattgtgt agctgatgat gattcttcag
       61 tcattgaaga ttgtgtagct gatgattctt ctgttgctga gctttgtgca gtagatgaag
      121 cttgagtagc ttgtgcttca gcctctgctt gttcgcgtgc ttctttttca gctttttctt
      181 gtgcagcttg ttgacgagct tgttctgcta gacgttgttg ttcacgaata cgagcttgct
      241 ctgcttcagc ttctgctttt tgacggttca agtcagcttt cttgtcttca gcagttgctt
      301 gttcagcagc taatgaagtt ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc
      361 ctttttgaga ttccaaagca gcttggtttt cagctaattc ttttagttta gcatcgtttt
      421 cagctttttt gtcttctaca gcttttttat cttgtttttg ttgttccatc aagtcgttgt
      481 ttgcttttac catcgttgtc attgcttgaa cgcgtccaat agcatctgct aatgaatcag
      541 cattcaatac agcatcgatg tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt
      601 gtttttggat tgtatcttca cgtttttcaa tacgttcttg taaatctgca atgtctttta
      661 ctaattgtgc agattcttga cgcaatgtat cttgttttgc taataggtct tgtgcttgtg
      721 tgttgatttc agaaacttgg ctttctaagg catcgatttg tgattgtgca tctgcttgtt
      781 gatttttcaa gtctgcaatc ttttgatctt gctgttgtat ctgagaatca aaatcgtccg
      841 ccgcggcagc gatcggtgat gctacggcag tcaaggtcat tgaacatacc attactgctg
      901 atattaaact ctttttcact cttcattcct ccgactggct taatttaata aatatctaaa
      961 tatatttaag tctttcttaa cgatataaaa attgtaccat agcagtgtgt caacgatata
     1021 atagttatgt tacaaaaatg tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc
     1081 agaaaatgat catgaaaaag ccgcgttaag tctagcattt cttaagataa caacgattct
     1141 ttttcgcttt cgacctaaaa ataaaaaaag aaattattca tcactgaata at
//
LOCUS       DAFBYI010000242         1189 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFBYI010000242 DAFBYI010000000
VERSION     DAFBYI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.254"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..630
                     /locus_tag="LLD04_002836"
     CDS             <1..630
                     /locus_tag="LLD04_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5649701.1"
                     /translation="PNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWR
                     LELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARH
                     TKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          369 a          194 c          290 g          336 t
ORIGIN      
        1 ccaaataaaa ttagccaatt tttggcgggt tcgatgaaga actttatcca tgatttattt
       61 ttagaaccac atttctcacg tgctgatatt gcttgcgaca ttatcgatgt gcctgatgat
      121 tttattacgc aatatcgtgt ggttgaccct gtttcattca aaccaatcta tgggcggtct
      181 gggaagctgg aaacggcata ttggggtagt cgtgccagtg agcgacaaat aaggatgtac
      241 aacaaaaaat tggaacaaga aaccaaacgg aaaattgtac caccggaaat aaaaacttgg
      301 tggcgactgg aattgcagtt acggcgtgga aaagctactg attggtatgc gatggttcat
      361 gaaagtttag acagtttcgc aagtcctcat tttttgccga ctgatacaaa gcatactgat
      421 agaattatga ttgcaggatt gatttcagat caaaatttat gggctggctt ggctcgtcat
      481 acaaaatata aatatcgaaa tttgttgaaa aaagaaagcc aaagtgatga gttagttaat
      541 catttacgag agacattttc ggaatcggca gatgttttga aaaaagaatt ggatacgtgg
      601 cttttggggt tggacgtgac ggaagaatga gtggatgaat ttttgtccac ttttttattt
      661 tcagcacaag tcggcatggt gggtcgtgcg tttgcagcaa gagcgaaaaa acgctaacga
      721 atccgcaaat gccgacttgg ataaaacagt tgcgcaaaga gacttggctt tttataataa
      781 atttagggat atcccagtgt cactctttgg ggtaattcgg gggaaggagt tacccctcac
      841 tctggaaaat tagagcgaag cagacgagat tatcccaatg aatatgacgg cttactcgga
      901 gtgataccga gtaaaaaagt cagataagtg agagtaaatt tacgtcaatg tttaagataa
      961 gcgattattt tgcgtgaagc aaaataaagc gtaaaaggag ttgagagcgt gacacgcttt
     1021 cggctccttt tttcgttgtc acttgtgaca gcgaaaaccc tcggagagcg tttggaattg
     1081 tgacagccat gtcacaatta ggaaaatgtt actctttgtc aaagagtaac agaaaggttt
     1141 acactctttt tattagcgac agcgcgaagg aggggttcac cttagggtt
//
LOCUS       DAFBYI010000243         1188 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFBYI010000243 DAFBYI010000000
VERSION     DAFBYI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.255"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            141..398
                     /locus_tag="LLD04_002837"
     CDS             141..398
                     /locus_tag="LLD04_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649702.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LLD04_002838"
     CDS             complement(630..1061)
                     /locus_tag="LLD04_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK5649703.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DAFBYI010000244         1173 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFBYI010000244 DAFBYI010000000
VERSION     DAFBYI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.256"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..336
                     /locus_tag="LLD04_002839"
     CDS             <1..336
                     /locus_tag="LLD04_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HBK5649704.1"
                     /translation="DFLKEKYADVEENQTALPSRFDELIEQKRELKRTKKEIESSIRQ
                     VDNEIISELGKREASIGITQRNIISWKLVRTRRMNTKKLAEKYPDVANDEEIYNVTES
                     RRLTEKEIK"
     gene            338..>1173
                     /locus_tag="LLD04_002840"
     CDS             338..>1173
                     /locus_tag="LLD04_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK5649705.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EE"
BASE COUNT          460 a          184 c          250 g          279 t
ORIGIN      
        1 gacttcttaa aagaaaaata tgcagatgta gaagaaaatc aaacagctct accatcacgt
       61 tttgatgaac ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga aattgaatca
      121 tctattcgtc aagtagacaa cgagatcatc agtgagctag gtaaacgtga agccagtatc
      181 ggtatcacac aaagaaacat tatcagctgg aaacttgttc gtacaagacg gatgaacacg
      241 aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta taacgttact
      301 gaatcaagaa ggctaaccga aaaggagatc aaataatatg gcaacaaatg aatcgttaaa
      361 aaatcaattg gcagaaaagc cacagaaaca agttgcacca ggacagttag ggcttaaagc
      421 tctaatgaat acaccaacaa tgagaaagaa atttgaagaa gtacttcatg acaatgctaa
      481 tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg cagatagtga
      541 accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa
      601 gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa caggaaaatg
      661 ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag cccaacgatc
      721 aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa aaagctggaa
      781 ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg aagtcattgg
      841 atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt ggaccaaaca
      901 agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa agttaagtgg
      961 tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa atcttctttc
     1021 taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg agagagtcca
     1081 aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag atattcctga
     1141 aagaaaagaa gcagaggtta tatctgaaga aaa
//
LOCUS       DAFBYI010000245         1172 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFBYI010000245 DAFBYI010000000
VERSION     DAFBYI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.257"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..>1172)
                     /locus_tag="LLD04_002841"
     rRNA            complement(<1..>1172)
                     /locus_tag="LLD04_002841"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          247 a          362 c          269 g          294 t
ORIGIN      
        1 accgcggcgt gctgatccgc gattactagc gattccggct tcatgcaggc gagttgcagc
       61 ctgcaatccg aactgagaga agctttaaga gattagctta gcctcgcgac ttcgcaactc
      121 gttgtacttc ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac
      181 gtcatcccca ccttcctccg gtttgtcacc ggcagtcttg ctagagtgcc caactgaatg
      241 atggcaacta acaataaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac
      301 gagctgacga caaccatgca ccacctgtca ctttgccccc gaaggggaag ctctatctct
      361 agagtggtca aaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca
      421 catgctccac cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt
      481 actccccagg cggagtgctt aatgcgttag ctgcagcact gaagggcgga aaccctccaa
      541 cacttagcac tcatcgttta cggcgtggac taccagggta tctaatcctg tttgctcccc
      601 acgctttcga gcctcagcgt cagttacaga ccagagagcc gccttcgcca ctggtgttcc
      661 tccatatatc tacgcatttc accgctacac atggaattcc actctcctct tctgcactca
      721 agtctcccag tttccaatga ccctccccgg ttgagccggg ggctttcaca tcagacttaa
      781 gaaaccgcct gcgctcgctt tacgcccaat aaatccggac aacgcttgcc acctacgtat
      841 taccgcggct gctggcacgt agttagccgt ggctttctgg ttagataccg tcaagggatg
      901 aacagttact ctcatccttg ttcttctcta acaacagagt tttacgatcc gaaaaccttc
      961 ttcactcacg cggcgttgct cggtcagact ttcgtccatt gccgaagatt ccctactgct
     1021 gcctcccgta ggagtttggg ccgtgtctca gtcccaatgt ggccgatcac cctctcaggt
     1081 cggctatgca tcgtggcctt ggtgagccgt tacctcacca actagctaat gcaccgcggg
     1141 tccatccatc agcgacaccc gaaagcgcct tt
//
LOCUS       DAFBYI010000246         1132 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFBYI010000246 DAFBYI010000000
VERSION     DAFBYI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.258"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            205..951
                     /gene="truA"
                     /locus_tag="LLD04_002842"
     CDS             205..951
                     /gene="truA"
                     /locus_tag="LLD04_002842"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5649706.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT          393 a          190 c          251 g          298 t
ORIGIN      
        1 aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc
       61 gagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc
      121 ttttatctga aaagaaagaa cgaaaaaatt ttataaaaaa atcattattc cagataacct
      181 tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc atacgacgga
      241 acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa
      301 aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca
      361 gatgccggag tacatgcgat tgggcaagta atccattttg attatccaga ggagcgtccg
      421 ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa
      481 gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa
      541 tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt
      601 ccttaccctc ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac
      661 gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaagtacg tacgatctac
      721 gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat
      781 ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc
      841 cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga
      901 cctacagcac atcctgaagg actttatttg tatgaagtga aatatgaata agaggttgtg
      961 acagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattactt ctcaaaattt
     1021 tgcaaaattt cggcttattt tcgaaggagc tacttctgga acaccgtgaa tcagaggttg
     1081 tgaaaaagct gccctgcatc aagtaataag aacaaccaaa caaaaatagc tc
//
LOCUS       DAFBYI010000247         1090 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFBYI010000247 DAFBYI010000000
VERSION     DAFBYI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.259"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            5..979
                     /gene="bsh"
                     /locus_tag="LLD04_002843"
     CDS             5..979
                     /gene="bsh"
                     /locus_tag="LLD04_002843"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5649707.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          383 a          135 c          187 g          385 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca
      961 aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag atataataca
     1021 aaaaggcaaa gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta
     1081 atgtttttaa
//
LOCUS       DAFBYI010000248         1082 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFBYI010000248 DAFBYI010000000
VERSION     DAFBYI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.260"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            700..>1082
                     /locus_tag="LLD04_002844"
     CDS             700..>1082
                     /locus_tag="LLD04_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5649708.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DAFBYI010000249         1080 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFBYI010000249 DAFBYI010000000
VERSION     DAFBYI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.261"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            336..>1080
                     /locus_tag="LLD04_002845"
     CDS             336..>1080
                     /locus_tag="LLD04_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5649709.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRS"
BASE COUNT          356 a          181 c          232 g          311 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
//
LOCUS       DAFBYI010000250         1074 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFBYI010000250 DAFBYI010000000
VERSION     DAFBYI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.262"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..243
                     /locus_tag="LLD04_002846"
     CDS             <1..243
                     /locus_tag="LLD04_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649710.1"
                     /translation="FNFTENKWEEAVTQDYSKKIELLENLSIGLQVDNTALKKSNEEL
                     TEKADSMVQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            243..380
                     /locus_tag="LLD04_002847"
     CDS             243..380
                     /locus_tag="LLD04_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5649711.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDEEADKIISKEESA
                     S"
     gene            418..711
                     /locus_tag="LLD04_002848"
     CDS             418..711
                     /locus_tag="LLD04_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649712.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKIAETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            734..931
                     /locus_tag="LLD04_002849"
     CDS             734..931
                     /locus_tag="LLD04_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5649713.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            942..>1074
                     /locus_tag="LLD04_002850"
     CDS             942..>1074
                     /locus_tag="LLD04_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649714.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     "
BASE COUNT          402 a          147 c          212 g          313 t
ORIGIN      
        1 tttaacttca ctgaaaataa gtgggaagaa gcagttacac aagattattc taaaaaaata
       61 gaactactag aaaatttatc tataggttta caggtagata atactgcatt gaaaaaatca
      121 aatgaagagt taacggaaaa agcagattcg atggtgcagt tgaacgccaa actgatgctt
      181 aatgatttaa caattaataa aaaaattgaa gcaatagaaa aacaaattgg aggtgcttca
      241 taatgtttag ttttagcgat gtaaaaatga tgtacgattg gggctgtttt actgacgatc
      301 aagttcgaca attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
      361 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
      421 attaatttag gagaatgggg aacaatcgca ggatcaatca ctgcgattgt ttctttgatt
      481 ttattagtaa taagaccagt cactgcatct ttctcgaaga ttgctgaaac tctttcaaaa
      541 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      601 atgattcatg aagaactaaa gaaacatgat gaaagattag atacacatgc agaaaaattg
      661 gtagaacaca cacaacaaat taaaacttta tttagagaaa gatctcggta aaaaagaaag
      721 gagttaagaa gaaatgattt tacccgataa gtattatcaa gttattaaat ggacggtttt
      781 aacagtttta ccagctgcat ctgctttagt agccatgtta ggcaaagcat atggatggaa
      841 tggaacagat atgacagttc tgactatcaa tgcagtagcc acgtttttag gtgttatcac
      901 tggtgtgtcg gcttataatt tgaaaaaata ggaggaaaca aatgaaaaag aaaattacta
      961 ttactgcgat gagcctgtta acggctcttt ttttattgcc gattaatggg tttgcctata
     1021 cgattaacaa tgaatttaat ttgggcccaa acgaaggtag ctcacaagta gcaa
//
LOCUS       DAFBYI010000251         1064 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFBYI010000251 DAFBYI010000000
VERSION     DAFBYI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.263"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..203
                     /locus_tag="LLD04_002851"
     CDS             <1..203
                     /locus_tag="LLD04_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5649715.1"
                     /translation="DKRTENQVRGMIEWSQHNVFWASNILSAKKLREKYDTMAAQANR
                     DYKTKQTKTLEYEKFSTDELPI"
     gene            218..>1064
                     /locus_tag="LLD04_002852"
     CDS             218..>1064
                     /locus_tag="LLD04_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5649716.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSI"
BASE COUNT          373 a          185 c          248 g          258 t
ORIGIN      
        1 tcgacaagcg gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt
       61 tttgggcatc gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag
      121 cgcaagcaaa tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta
      181 gcacagatga gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat
      241 ggaaaagctg atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc
      301 tttgtatgga tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat
      361 gcaatgtggg tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa
      421 cgagagcctg aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca
      481 agcgttgttt gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc
      541 ggttgaagta accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt
      601 tttaactgga aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt
      661 cttgaagcga tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga
      721 tcaaatcaaa ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat
      781 tgcagatatc aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa
      841 agatgcatca gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg
      901 acagaaccta gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta
      961 cggtacgaga acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc
     1021 tcaaacagca gacaagcgca taaaaccagt gaaaggtagt atcg
//
LOCUS       DAFBYI010000252         1020 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFBYI010000252 DAFBYI010000000
VERSION     DAFBYI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.264"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            73..>1020
                     /locus_tag="LLD04_002853"
     CDS             73..>1020
                     /locus_tag="LLD04_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5649717.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLS"
BASE COUNT          353 a          175 c          189 g          303 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct
//
LOCUS       DAFBYI010000253          891 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFBYI010000253 DAFBYI010000000
VERSION     DAFBYI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.265"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            10..>891
                     /locus_tag="LLD04_002854"
     CDS             10..>891
                     /locus_tag="LLD04_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBK5649718.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEENLDKIIDK
                     IRQKYGFTSIVHASSMLE"
BASE COUNT          320 a          144 c          194 g          233 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacacg aagtcaacgg
       61 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgattgct
      121 acagtaggtg tcggagataa tccccttttg gctaaactag ccttagacaa cgaagcaaag
      181 cataatgaag gatttgttgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc cttatacgat taagaataga
      361 ctaggtgtga ttggcttaca gctgtacttc catgctaacg gaatcgatcg aacagatata
      421 gcaatcccgc cagaaccgac aaaggaaaaa tcttatggta attcacaagt gttaccacga
      481 gattatacta gaagaaatga aatcgagctg gtggtaaaag aaatggcgga acaagtagcc
      541 attcgtatca ggcagcataa ttgtaaaaca ggatgtgttc atttaaacat tggtacgtcg
      601 atcttagaaa cgagaccagg attttcacac cagatgaaaa tacctatcac agataataca
      661 aaggaattgc agaattattg tcttttcttg tttgataagt attatgaagg acaagaagta
      721 aggcacgttg gaataaccta tagcaagctt gtgtacaccg attcgcttca gctggattta
      781 tttagtgatc cacaaaagca aattaacgaa gaaaatttag ataaaattat tgacaagatt
      841 cggcaaaaat atggttttac gtcgattgta catgcttctt caatgttaga a
//
LOCUS       DAFBYI010000254          890 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFBYI010000254 DAFBYI010000000
VERSION     DAFBYI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.266"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..183)
                     /locus_tag="LLD04_002855"
     CDS             complement(<1..183)
                     /locus_tag="LLD04_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649719.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATP"
BASE COUNT          250 a          201 c          110 g          329 t
ORIGIN      
        1 aggtgttgct gtttcttcta atagtgtact taattcctta atattttgtt ttccttcttc
       61 taacatccaa ctactcgctt tttcttcgcc ttcggtaaca aaaggcgcca ctcccttcgc
      121 ccatgttgct cgcccacata agacaccatt ctccataaag gacaacgcgg agataccacc
      181 catctaaata acatccataa acctttctag tagaagaata gtgatagtat ccatcccttt
      241 ttctactggg aatacaatta cacgattcaa agcttgatac acaacatttc ccttcttata
      301 aaccgctatt gtaaccgaat tgttttgaat cacctttttg ggatagaaac tggctccttt
      361 ccgcaacttc ttaaatgttg gtttctttta tactttcttt gtagcaacta caaatttctt
      421 caaatctcta aatgtttgca taacaaccgc ctttctgact tttactcagg atggtattta
      481 aaaatcagca aaataaggct tctctccatc ttttttattt cctagctgct ttaaagacac
      541 tcttgctgtt ttttaatctc tatcaagggg gctctgaatc tattttgatg aacttatctt
      601 attaatttct caaatgagct gtcagacaca actgtatttt ttcaatctca taccggacaa
      661 ctatgccagc ttaaaaaaat ttcctctggc aaaacttctt tattgaaaaa tgacaatgaa
      721 aaaagaccat tctttttgtt gaatgctcat tgtgtaaaca tatttcctgc gactgctctt
      781 cgaaccattc tcagtgcaat ccctaattta tctattaaat cttcttttag ttcctcatct
      841 ttcatattca aaatttcctt tgtatgttac acaagtttgt tctctaaata
//
LOCUS       DAFBYI010000255          837 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFBYI010000255 DAFBYI010000000
VERSION     DAFBYI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.267"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            24..716
                     /locus_tag="LLD04_002856"
     CDS             24..716
                     /locus_tag="LLD04_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5649720.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          291 a          160 c          166 g          220 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttt
//
LOCUS       DAFBYI010000256          807 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFBYI010000256 DAFBYI010000000
VERSION     DAFBYI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.268"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..672
                     /locus_tag="LLD04_002857"
     CDS             <1..672
                     /locus_tag="LLD04_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5649721.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          152 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcagg
//
LOCUS       DAFBYI010000257          807 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFBYI010000257 DAFBYI010000000
VERSION     DAFBYI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.269"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..783
                     /locus_tag="LLD04_002858"
     CDS             <1..783
                     /locus_tag="LLD04_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5649722.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          247 a          172 c          181 g          207 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaac
//
LOCUS       DAFBYI010000258          794 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFBYI010000258 DAFBYI010000000
VERSION     DAFBYI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.270"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            3..695
                     /locus_tag="LLD04_002859"
     CDS             3..695
                     /locus_tag="LLD04_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649723.1"
                     /translation="MQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDP
                     TRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPKR"
BASE COUNT          282 a          145 c          151 g          216 t
ORIGIN      
        1 ctgtgcagat tgagttacaa cacacacatg ataaagcaac gtttgagaaa aactacaagg
       61 catacgttga attggctaga gattcagcaa tgaaatatgg tattccatta acgttggaca
      121 ctccttataa ccaaccggga atcaaatcgc atttatgggt aacacaaaac atctggggcg
      181 atcatacaga tccttacggt tatctttctg aaatgggcgt aagtaaagaa aaattagcat
      241 atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac aagcctgtca
      301 ttgatccaac acgagctggt gcagctaatc ctacactgac agatggaacg aattacgccc
      361 acattgatca gtttggagaa atcgaaaatg caaatttgca tgtagctgga tggcacattg
      421 ctaactataa atacgagtat atcttcatta tggattacaa tactgggaaa gaattagctc
      481 gagtaagagc tgatggaatt tatagatcag atgtaaatca agcttataat acttctggaa
      541 atgttggcta tcatgtatct tttaacatgc gtaacttccc taataagaaa gtctatgtca
      601 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
      661 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcatt
      721 acataactat attgacaact ataaaaatca ttcgataaaa tagtgatgtt atcgcatatc
      781 ttcactatca ccca
//
LOCUS       DAFBYI010000259          789 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFBYI010000259 DAFBYI010000000
VERSION     DAFBYI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.271"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..779
                     /locus_tag="LLD04_002860"
     CDS             <1..779
                     /locus_tag="LLD04_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5649724.1"
                     /translation="MLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGV
                     SLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGEL
                     ISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLI
                     KNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIP
                     LKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          101 g          243 t
ORIGIN      
        1 atatgctaga ttactactat tttgaaaaaa atttatcata taattatgta atacatttaa
       61 aaacttttac aggaatgatt tttaaattcg ccaaccgtcg atatggatta aaatataatc
      121 ccgtaaatgg ggtttcttta gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa
      181 aagatggtta tttatcaaaa gaagaagttc aaatgattgc tagtaaacaa aaaagttcac
      241 atcaacagtc acgttattct ttaattacta aatttttgtt tttaactgga ctacgttatg
      301 gtgaattgat atctttgact gaatctgatt atgatggtaa taaaattacg gtttctggta
      361 cttatgatta cgagttaaaa attaaaacaa caactaaaaa tacaggttct tatagaacca
      421 ttgaattatc aagtaatgct aaagaaatca tagatcaatt aattgaagaa aacaaattaa
      481 ttaagaataa taaagataaa tacattttca tctctaaaaa tggtaacccg atatctattc
      541 aagcatataa tcaatcttta agagcagtat ccaaagaatt aaacatagat aaaaaagttt
      601 cttctcacat gttaaggcat tcccacattt ctctacttac agaattagga atcccattaa
      661 aagccattat ggatcgtgta ggacatgaag attcaaaaac aacattaaag atttatactc
      721 atacaactaa aaatatgcaa aatcaactag tagaaaaact aggaaaaata gaaatttaaa
      781 ttgcccctt
//
LOCUS       DAFBYI010000260          774 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFBYI010000260 DAFBYI010000000
VERSION     DAFBYI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.272"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(248..727)
                     /locus_tag="LLD04_002861"
     CDS             complement(248..727)
                     /locus_tag="LLD04_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649725.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DAFBYI010000261          767 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFBYI010000261 DAFBYI010000000
VERSION     DAFBYI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.273"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..339)
                     /locus_tag="LLD04_002862"
     CDS             complement(<1..339)
                     /locus_tag="LLD04_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5649726.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LLD04_002863"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LLD04_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK5649727.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          230 a          124 c          124 g          289 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatat
//
LOCUS       DAFBYI010000262          708 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFBYI010000262 DAFBYI010000000
VERSION     DAFBYI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.274"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            14..538
                     /locus_tag="LLD04_002864"
     CDS             14..538
                     /locus_tag="LLD04_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649728.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            566..>708
                     /locus_tag="LLD04_002865"
     CDS             566..>708
                     /locus_tag="LLD04_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649729.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFG"
BASE COUNT          242 a          114 c          163 g          189 t
ORIGIN      
        1 tggggacggt ttaatggctg atattgtgca attaaaagaa gacggagttg ctaagtattt
       61 gaaaactcat gccaaaggaa ttgatggtgt ggagggtgtt ttagtaaaag ccacaggtaa
      121 tgaaacggtc ttaggaacga aaaattttaa agacggattg caattcaatg gattgcctgt
      181 gcaagcaggt atgatcgagc gagctatcac actagctgat cgaagtgata caacaaacgt
      241 aactgatgtg aatggaaaaa taatcagaat cggaaacatc gtatttctta cttttaattt
      301 taaatgtggc acatggcccg aaggatcaga aactcgttgg attttaaaaa tcccggacgg
      361 tttcaaacgt gatcaaggct atccagcgca aaccgcgctt tcactagtta gaaatgcaag
      421 tcagccagcg gatgcacgtg cattcattga tcaaagtagc atcatacaag ccaaatcagg
      481 ttcaggtagt tcatatattt caggtatgtg gatcactcaa gatccttggc cggcttaaca
      541 gattactaat tggaggaaga gtaaaatgaa aacaatttat aaagtattgt atccaatggg
      601 atatgaagaa catcaagtag aagataactt tccaacatct ttaccatttg tcgaagttcc
      661 acctattttg tttgaaaaga aggaagatga aacggatgaa ggtttcgg
//
LOCUS       DAFBYI010000263          666 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFBYI010000263 DAFBYI010000000
VERSION     DAFBYI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.275"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..165
                     /locus_tag="LLD04_002866"
     CDS             <1..165
                     /locus_tag="LLD04_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649730.1"
                     /translation="KKLPDGTELVIRQSYLKDMVAPVLIDRFNVTDSVVENHLKEFRS
                     SIDNTFRLGS"
     gene            172..375
                     /locus_tag="LLD04_002867"
     CDS             172..375
                     /locus_tag="LLD04_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK5649731.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            372..>666
                     /locus_tag="LLD04_002868"
     CDS             372..>666
                     /locus_tag="LLD04_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649732.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
BASE COUNT          250 a          100 c          145 g          171 t
ORIGIN      
        1 aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa agatatggta
       61 gcacctgtat taattgatcg ttttaacgtg actgattctg tggtagagaa ccacttaaaa
      121 gaatttcgat caagtataga caacactttc agattaggga gttgattgac aatgaacacc
      181 agacaccgca gagtagcaaa actaagaaaa caggaactaa atgtactaaa gacaaagttt
      241 gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca gtttgttgct
      301 gatgcaagtg atgctattcg taagtttggg atttcgatat taaatgatga tcgtaaatgg
      361 gaggaaatga gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt
      421 taatgctaga tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg
      481 gtgacatggt ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa
      541 agatactgtc aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga
      601 ctgcaaaaca caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga
      661 gcaaat
//
LOCUS       DAFBYI010000264          642 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFBYI010000264 DAFBYI010000000
VERSION     DAFBYI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.276"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            81..467
                     /locus_tag="LLD04_002869"
     CDS             81..467
                     /locus_tag="LLD04_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5649733.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          242 a          100 c          128 g          172 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtataataaa
       61 aataaaaaag gagatctttc atgaaaaaaa ttttagggtt gatcgttatc atattgattg
      121 cttttatagg atggaaaggt tgggactact atcagtctac ttatgtaggt aaagattatt
      181 atgcggtagt caaagctcca atgcctgcag aaactgatat caaagcagat aatggggaag
      241 taattggaag aggttttaaa tacaacgtag atgcttatgc tgaaaacggt gaaaaaagac
      301 agttagattt cgatgtcatt acttctggtg actctgccaa tggttctgct taccccgaag
      361 gaacgatctt gcagttgaaa gcaagcgaaa agcggatcat agagaaaaaa gtaattacca
      421 gcgataaggt accctcatct gtaaaagaaa aattaggcct atcttaagct aagaatatat
      481 cgcaaaaaag cactcgaagt gagtgctttt ttgcttactg attctagaaa acaaaaaaac
      541 agaattaacc ttcgaagtca gcctcactgt tcttaaagtc ctaaaacaaa ctaaagagca
      601 aggcaatcaa tcgatttcta tacaaacaag ggactgtgac aa
//
LOCUS       DAFBYI010000265          556 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFBYI010000265 DAFBYI010000000
VERSION     DAFBYI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.277"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            29..>556
                     /locus_tag="LLD04_002870"
     CDS             29..>556
                     /locus_tag="LLD04_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK5649734.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSK"
BASE COUNT          205 a          107 c           88 g          156 t
ORIGIN      
        1 accaattcac agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac
       61 tctagtcact aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa
      121 cacactccta cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg
      181 ttttaattca ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg
      241 tgaattaaca ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc
      301 agcctacaaa agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg
      361 tgttacgatg tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc
      421 acaaaccatg tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag
      481 agccttaaat gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg
      541 tcaaaccgta tccaaa
//
LOCUS       DAFBYI010000266          555 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFBYI010000266 DAFBYI010000000
VERSION     DAFBYI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.278"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(271..534)
                     /locus_tag="LLD04_002871"
     CDS             complement(271..534)
                     /locus_tag="LLD04_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HBK5649735.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          215 a           97 c           75 g          168 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctctattt tttta
//
LOCUS       DAFBYI010000267          518 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFBYI010000267 DAFBYI010000000
VERSION     DAFBYI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.279"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(44..>518)
                     /locus_tag="LLD04_002872"
     CDS             complement(44..>518)
                     /locus_tag="LLD04_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBK5649736.1"
                     /translation="HYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKL
                     FHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFET
                     LAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          152 a          122 c           90 g          154 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgc
//
LOCUS       DAFBYI010000268          509 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFBYI010000268 DAFBYI010000000
VERSION     DAFBYI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.280"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DAFBYI010000269          509 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFBYI010000269 DAFBYI010000000
VERSION     DAFBYI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.281"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..132)
                     /locus_tag="LLD04_002873"
     CDS             complement(<1..132)
                     /locus_tag="LLD04_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5649737.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAFBYI010000270          488 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFBYI010000270 DAFBYI010000000
VERSION     DAFBYI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.282"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..414)
                     /locus_tag="LLD04_002874"
     CDS             complement(<1..414)
                     /locus_tag="LLD04_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649738.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DAFBYI010000271          480 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFBYI010000271 DAFBYI010000000
VERSION     DAFBYI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.283"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..143)
                     /locus_tag="LLD04_002875"
     CDS             complement(<1..143)
                     /locus_tag="LLD04_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5649739.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQ"
     gene            complement(154..360)
                     /locus_tag="LLD04_002876"
     CDS             complement(154..360)
                     /locus_tag="LLD04_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5649740.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          137 a          106 c           66 g          171 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      121 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      181 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      241 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      301 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      361 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      421 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
//
LOCUS       DAFBYI010000272          478 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFBYI010000272 DAFBYI010000000
VERSION     DAFBYI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.284"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT          142 a          116 c           80 g          140 t
ORIGIN      
        1 attttgttcc tcctcaaaga aaaagagact tttggaaaga atttccagag ttcacgtaac
       61 cctaaaaatt catatccaaa aatctctagc ttgaagaaac ctaaaaatat tcgtataatg
      121 aacctgagtt acggagctag gtgaagtagt ttccgtaaat agtcgagagt gttagcgcac
      181 tgctcggctt ttattttgct cttttttctc gttttctttg attatagcac tcctcctaaa
      241 aaaataaaaa gacctcaggg aatttcattc cccgaacccc ttgagcctcc tcctgccgaa
      301 atggaaaggg cgcacccgct gtttatttca ttcagctatc ggctttcaca atctaacacg
      361 atcgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg tgttttaacg
      421 attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa aatatgcc
//
LOCUS       DAFBYI010000273          455 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFBYI010000273 DAFBYI010000000
VERSION     DAFBYI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.285"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..>455)
                     /locus_tag="LLD04_002877"
     CDS             complement(<1..>455)
                     /locus_tag="LLD04_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK5649741.1"
                     /translation="HLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAE
                     ISVKTVPSAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTE
                     AENFRKKMRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPT"
BASE COUNT          113 a          112 c           73 g          157 t
ORIGIN      
        1 ggttggctca tccaaaaaaa gaatatcggg ttgtgtcatc agccacttgg ataaaacgac
       61 tttttgttga ttcccaccac taagactact gacaatttga tggatggagt ttgtttttat
      121 acgcatcttt ttccggaaat tttctgcctc agttacttct ttttctttat ctaatactcc
      181 tttttgactt attttatcca aactgctcag cgtcacgttt tcccgaatat ccatcagcag
      241 attcagtcct aaagattttc ggtcctccga gacataagcg agtccgtttg caatggctga
      301 aggaacggtc ttaactgata tctctgcccc ttcctttaat atacgtccgg atatcttgct
      361 tccataagaa tgtccaaata gactcatagc gaattcagtt ctgcccgctc ccatcagtcc
      421 agctattcct acaatctctc cttttttcaa atgca
//
LOCUS       DAFBYI010000274          430 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFBYI010000274 DAFBYI010000000
VERSION     DAFBYI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.286"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..>430
                     /locus_tag="LLD04_002878"
     CDS             <1..>430
                     /locus_tag="LLD04_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBK5649742.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAFBYI010000275          429 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFBYI010000275 DAFBYI010000000
VERSION     DAFBYI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.287"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(12..392)
                     /locus_tag="LLD04_002879"
                     /pseudo
     CDS             complement(12..392)
                     /locus_tag="LLD04_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
BASE COUNT          104 a           85 c           55 g          185 t
ORIGIN      
        1 ttttacactc tcatcggacc tgtcacaagc attacaagta caaaaataat tagcacaaaa
       61 aatatatttg taacgccttt tgtcatcact tttagcgctg tattcgattt atcaaccact
      121 ttgttatggt cttttatttt actttctaag atttctttat actcttttgt ttcttctctc
      181 gcttgtttcg tcacttcacg tatttcgtct ttagtatcgc ttaattcatc ttgaatagct
      241 tgttgaaaat ctaatttcaa attatcttca tcaagtcgtt taatatagtg ctttgcagtt
      301 gtttcaaagt ttaatcttgc atgcttagtc ttttcttcaa aaacatctaa cttattatta
      361 aagttcgagg tcgaagcctc tagtcttttc atgagtatgt ttgttctctc gttcctgtct
      421 tgctcgctc
//
LOCUS       DAFBYI010000276          409 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFBYI010000276 DAFBYI010000000
VERSION     DAFBYI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.288"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..386
                     /locus_tag="LLD04_002880"
     CDS             <1..386
                     /locus_tag="LLD04_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5649743.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           74 c           64 g          113 t
ORIGIN      
        1 accgaaaaga aatctgtgat gactttaagg ctgtttatca agctaactca aaagaagaag
       61 cgaatacctt cttatccggc atgattgaga aatggaagaa aaactatcct aaagtgacgc
      121 agtcactcat agaaaaccaa gacttattaa ctttttatga ttttccacct agcattcgta
      181 gaaccattta ctcaaccaat ctaatcgagt ctttcaataa gcaaattaaa agatacagcc
      241 gtagaaaaga gcagtttcaa aatgaagaat cactagaacg ctttctagtc agcatttttg
      301 atacatacaa tcaaaaattt ctaaacagaa gccataaagg ttttcaacag gtaaccgata
      361 cattagtttc aatgtttact gagtaactaa ttattttgca ggaggacaa
//
LOCUS       DAFBYI010000277          409 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFBYI010000277 DAFBYI010000000
VERSION     DAFBYI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.289"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..379
                     /locus_tag="LLD04_002881"
     CDS             <1..379
                     /locus_tag="LLD04_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBK5649744.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAFBYI010000278          372 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFBYI010000278 DAFBYI010000000
VERSION     DAFBYI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.290"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            82..>372
                     /locus_tag="LLD04_002882"
     CDS             82..>372
                     /locus_tag="LLD04_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK5649745.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          132 a           61 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cc
//
LOCUS       DAFBYI010000279          361 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFBYI010000279 DAFBYI010000000
VERSION     DAFBYI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.291"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DAFBYI010000280          354 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFBYI010000280 DAFBYI010000000
VERSION     DAFBYI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.292"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..>354
                     /locus_tag="LLD04_002883"
     CDS             <1..>354
                     /locus_tag="LLD04_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5649746.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA
                     NLPNILGDKGYISHK"
BASE COUNT          128 a           64 c           57 g          105 t
ORIGIN      
        1 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
       61 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      121 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      181 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      241 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      301 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat aaaa
//
LOCUS       DAFBYI010000281          347 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFBYI010000281 DAFBYI010000000
VERSION     DAFBYI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.293"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(122..206)
                     /locus_tag="LLD04_002884"
     tRNA            complement(122..206)
                     /locus_tag="LLD04_002884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Leu,seq:caa)
     gene            complement(227..297)
                     /locus_tag="LLD04_002885"
     tRNA            complement(227..297)
                     /locus_tag="LLD04_002885"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Cys,seq:gca)
BASE COUNT          105 a           86 c           71 g           85 t
ORIGIN      
        1 aatcgaaccc acgaatgccg aagccacaat ccggtgcgtt aaccacttcg ccacattcgc
       61 catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca
      121 ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc
      181 gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga
      241 tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt
      301 atactattat caatctataa aataaagaat aaattataga aactggg
//
LOCUS       DAFBYI010000282          341 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFBYI010000282 DAFBYI010000000
VERSION     DAFBYI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.294"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..>341
                     /locus_tag="LLD04_002886"
     CDS             <1..>341
                     /locus_tag="LLD04_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649747.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAFBYI010000283          327 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFBYI010000283 DAFBYI010000000
VERSION     DAFBYI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.295"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT          144 a           61 c           57 g           65 t
ORIGIN      
        1 aacagcggtt ctgtatggag atggctaaaa caatttgaaa aaaacggaat gttgaacatc
       61 aaatcaacta cgaaatacag cgttatatca ataaaaaact ggtctttgta ccaaagcagt
      121 gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac aagtgcacac aaacaagaat
      181 gaaaagaatt ataagaatga aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag
      241 cgtgtttacg acaccgactc agtttactac attctcgcgg aggagttatt caaacagatt
      301 tgtcagaatc aggaaatcaa aaagccg
//
LOCUS       DAFBYI010000284          305 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFBYI010000284 DAFBYI010000000
VERSION     DAFBYI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.296"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            83..271
                     /locus_tag="LLD04_002887"
     CDS             83..271
                     /locus_tag="LLD04_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649748.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          114 a           53 c           48 g           90 t
ORIGIN      
        1 aatttaaaca tttgacaaac tatagaacag cacttaaaat gtgtatacaa gtgacagcgc
       61 tttaaaaaaa gaggtgcagc ctatgttaaa aaaaactatg ttggtactag gttctatttc
      121 tctaggagta ttaggtttta gccccatgct attatccgta attggaaaaa ataattccaa
      181 aggggaactt aaattaaatc caaattacta caatagtcct aatgaaccta tgtttatttc
      241 agaaaaacaa aacgacggtc aggacaaata atcctgccgt cgttttgctc taacttctac
      301 cagta
//
LOCUS       DAFBYI010000285          305 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFBYI010000285 DAFBYI010000000
VERSION     DAFBYI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.297"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT          100 a           57 c           59 g           89 t
ORIGIN      
        1 gagaaaaagc atggaatgcg ctgcatcagg tatcgaggct tagaaaaaaa tcaggtgcat
       61 acgatgcttg tttttgccga aatgagtctg aaaaaattgg tcacttagca gtgggagcat
      121 atgtcttcat attttttatt atacaaaaat agacaaaagt atcagtctac actcttagta
      181 aagaggaaac tgatactttt gtctacagta tgatatttct tacatctact tttactccgg
      241 cagtaggact cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct
      301 atgcc
//
LOCUS       DAFBYI010000286          299 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFBYI010000286 DAFBYI010000000
VERSION     DAFBYI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.298"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..>299
                     /locus_tag="LLD04_002888"
     CDS             <1..>299
                     /locus_tag="LLD04_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK5649749.1"
                     /translation="KTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQERFELMPKF
                     ELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQVPLILA"
BASE COUNT          105 a           50 c           67 g           77 t
ORIGIN      
        1 aaaactgttc atcgaggtat caagtttgat tctatcgcag aagcagagta ttacgatcta
       61 gccttgtggc aagctgaagc gaatggctgg aaagtaaaac ttcaggaaag atttgagctg
      121 atgccgaaat ttgaactaga cggaaagaag tatcgcaaga tcgagtatat tcccgacttc
      181 acattttata aaaacggcaa acttgtcaaa gtcgtagatg tcaaaggaat gcagacaaaa
      241 gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagc
//
LOCUS       DAFBYI010000287          291 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFBYI010000287 DAFBYI010000000
VERSION     DAFBYI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.299"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            76..>291
                     /locus_tag="LLD04_002889"
     CDS             76..>291
                     /locus_tag="LLD04_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5649750.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DAFBYI010000288          260 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFBYI010000288 DAFBYI010000000
VERSION     DAFBYI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.300"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
BASE COUNT           82 a           48 c           48 g           82 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca
      121 tttaactccg aatcacaaga aataatttca aaaacaactt ctcctgtttt tggtttggtt
      181 ggcttattat cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata
      241 ggtcacaccc cttttttatt
//
LOCUS       DAFBYI010000289          254 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFBYI010000289 DAFBYI010000000
VERSION     DAFBYI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.301"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            46..>254
                     /locus_tag="LLD04_002890"
     CDS             46..>254
                     /locus_tag="LLD04_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5649751.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNA"
BASE COUNT           96 a           36 c           62 g           60 t
ORIGIN      
        1 gcacacttgt tggtggtcat gcgggcggaa atgggggaat taaaaatgat tgattacaat
       61 gatcgtggac gtttaaagtg ggcagggttt tatttatctg atcatactga agaaattgat
      121 gccgacaatg aacaacgaac cagtcaaaat ttagcaaaag aacaaatgac gactgaagag
      181 attgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatctat tcaaaaagaa
      241 gaacgaaacg ctga
//
LOCUS       DAFBYI010000290          250 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFBYI010000290 DAFBYI010000000
VERSION     DAFBYI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.302"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..>250
                     /locus_tag="LLD04_002891"
     CDS             <1..>250
                     /locus_tag="LLD04_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5649752.1"
                     /translation="SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF
                     QLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPC"
BASE COUNT           70 a           57 c           45 g           78 t
ORIGIN      
        1 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca
       61 tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat tcatgacagt
      121 caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat caattgtgtt
      181 gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca tttgaacctt
      241 cgaccttgtt
//
LOCUS       DAFBYI010000291          250 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFBYI010000291 DAFBYI010000000
VERSION     DAFBYI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.denovo.303"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..158
                     /locus_tag="LLD04_002892"
     CDS             <1..158
                     /locus_tag="LLD04_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBK5649753.1"
                     /translation="KNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYANY
                     KLSIVID"
BASE COUNT          104 a           28 c           37 g           81 t
ORIGIN      
        1 ttaaaaatca tgaaaaattc ttgattagaa attataagat tagtggaggg ggctgttatc
       61 ttttggaatg tagatttact tcaaaaaaat caatgaacag ctttttggaa cagcttaatg
      121 aatatgcgaa ttataaatta tctattgtta tagattaata ataaaactag cagatggtaa
      181 aaatactaat atgcgagaca cacattcaac cataactata taacttatca atttaaaact
      241 aaactgatgg
//
LOCUS       DAFBYI010000292         6675 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.126, whole genome shotgun sequence.
ACCESSION   DAFBYI010000292 DAFBYI010000000
VERSION     DAFBYI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.126"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            109..192
                     /locus_tag="LLD04_002893"
     CDS             109..192
                     /locus_tag="LLD04_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK5649754.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="LLD04_002894"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="LLD04_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK5649755.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="LLD04_002895"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="LLD04_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="LLD04_002896"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="LLD04_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="LLD04_002897"
     CDS             2348..2593
                     /locus_tag="LLD04_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5649756.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="LLD04_002898"
     CDS             2696..3565
                     /locus_tag="LLD04_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK5649757.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="LLD04_002899"
     CDS             3546..4280
                     /locus_tag="LLD04_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5649758.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="LLD04_002900"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="LLD04_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="LLD04_002901"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="LLD04_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBK5649759.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="LLD04_002902"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="LLD04_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBK5649760.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2343 a         1003 c         1395 g         1934 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaa
//
LOCUS       DAFBYI010000293         6302 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.131, whole genome shotgun sequence.
ACCESSION   DAFBYI010000293 DAFBYI010000000
VERSION     DAFBYI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.131"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..113
                     /locus_tag="LLD04_002903"
     CDS             <1..113
                     /locus_tag="LLD04_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5649761.1"
                     /translation="TVSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            358..474
                     /locus_tag="LLD04_002904"
     CDS             358..474
                     /locus_tag="LLD04_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5649762.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            490..2409
                     /gene="tet(M)"
                     /locus_tag="LLD04_002905"
     CDS             490..2409
                     /gene="tet(M)"
                     /locus_tag="LLD04_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5649763.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2507..3880
                     /gene="tet(L)"
                     /locus_tag="LLD04_002906"
     CDS             2507..3880
                     /gene="tet(L)"
                     /locus_tag="LLD04_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK5649764.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            4444..5705
                     /locus_tag="LLD04_002907"
                     /pseudo
     CDS             4444..5705
                     /locus_tag="LLD04_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            5950..>6302
                     /locus_tag="LLD04_002908"
     CDS             5950..>6302
                     /locus_tag="LLD04_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBK5649765.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFL"
BASE COUNT         2135 a          909 c         1303 g         1955 t
ORIGIN      
        1 tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg caggtatctc
       61 aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat
      121 tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt
      181 cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa
      241 atatgcggca aggtattctt aaataaactg tcaatttgat agtgggaaca aataattgga
      301 tgtccttttt taggagggct tagttttttg cacccagttt aagaatacct ttatcatgtg
      361 attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat
      421 cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt ttgaatggag
      481 gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc gggaaaaact
      541 accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac
      601 aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag
      661 acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga
      721 catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta
      781 ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg
      841 aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta
      901 tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag
      961 gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg
     1021 gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca
     1081 ttggaactcg aacaagagga aagcataaga tttcagaatt gctccttgta ccctgtttat
     1141 catggaagcg caaaaagcaa catagggatt gagcagctta tagaagtgat aacgaataaa
     1201 ttttattcat caacatacag aaagaagtct gaactttgcg gaaatgtctt caaaattgaa
     1261 tattcggaag aaagacaacg tcttgcatat gtacgccttt atggcggaat cctgcatttg
     1321 cgggattcgg ttagaatatc ggaaaaggaa aaaataaaaa ttacagaaat gtatacttca
     1381 ataaatggtg aattatgtaa aattgataag gcttattccg gggaaattgt tattttgcaa
     1441 aatgagtttt tgaagctaaa tagtgttctt ggagatacaa agctattgcc acagagagag
     1501 agaattgaaa atccgctccc tctgctgcaa acaactgttg aaccgagcaa acctcaacaa
     1561 agggaaatgt tacttgatgc acttttagaa atctccgaca gtgacccgct tctacaatat
     1621 tatgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
     1681 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
     1741 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
     1801 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
     1861 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt
     1921 atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt
     1981 aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg
     2041 atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag
     2101 ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat
     2161 gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt
     2221 agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca
     2281 aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct
     2341 gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa
     2401 ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat
     2461 attttttaat aaagatttga attaaagtgt aaaggaggaa ttaattatga agtgtaatga
     2521 atgtaacagg gttaaataca accaaatttt aatttggctt tgcgttttat ctttttttag
     2581 cgtattaaat gaaatggttt tgaacgtctc attacctgat attgcaaatg attttaataa
     2641 accacctgcg agtacaaact gggtgaacac agcctttatg ttaacctttt ccattggaac
     2701 agctgtatat ggaaagctat ctgatcaatt aggcatcaaa aggttactcc tatttggaat
     2761 tataataaat tgtttcgggt cggtaattgg gtttgttggc cattctttct tttccttact
     2821 tattatggct cgttttattc aaggggctgg tgcagctgca tttccagcac tcgtaatggt
     2881 tgtagttgcg cgctatattc caaaggaaaa taggggtaaa gcatttggtc ttattggatc
     2941 gatagtagcc atgggagaag gagtcggtcc agcgattggt ggaatgatag cccattatat
     3001 tcattggtcc tatcttctac tcattcctat gataacaatt atcactgttc cgtttcttat
     3061 gaaattatta aagaaagaag taaggataaa aggtcatttt gatatcaaag gaattatact
     3121 aatgtctgta ggcattgtat tttttatgtt gtttacaaca tcatatagca tttcttttct
     3181 tatcgttagc gtgctgtcat tcctgatatt tgtaaaacat atcaggaaag taacagatcc
     3241 ttttgttgat cccggattag ggaaaaatat accttttatg attggagttc tttgtggggg
     3301 aattatattt ggaacagtag cagggtttgt ctctatggtt ccttatatga tgaaagatgt
     3361 tcaccagcta agtactgccg aaatcggaag tgtaattatt ttccctggaa caatgagtgt
     3421 cattattttc ggctacattg gtgggatact tgttgataga agaggtcctt tatacgtgtt
     3481 aaacatcgga gttacatttc tttctgttag ctttttaact gcttcctttc ttttagaaac
     3541 aacatcatgg ttcatgacaa ttataatcgt atttgtttta ggtgggcttt cgttcaccaa
     3601 aacagttata tcaacaattg tttcaagtag cttgaaacag caggaagctg gtgctggaat
     3661 gagtttgctt aactttacca gctttttatc agagggaaca ggtattgcaa ttgtaggtgg
     3721 tttattatcc atacccttac ttgatcaaag gttgttacct atggaagttg atcagtcaac
     3781 ttatctgtat agtaatttgt tattactttt ttcaggaatc attgtcatta gttggctggt
     3841 taccttgaat gtatataaac attctcaaag ggatttctaa atcgttaagg gatcaacttt
     3901 gggagagagt tcaaaattga tccttttttt ataacaggaa ttcaaatctt tttgttccat
     3961 taaagggcgc gattgctgaa taaaagatac gagagacctc tcttgtatct tttttatttt
     4021 gagtggtttt gtccgttaca ctagaaaacc gaaagacaat aaaaatttta ttcttgctga
     4081 gtctggcttt cggtaagcta gacaaaacgg acaaaataaa aattggcaag ggtttaaagg
     4141 tggagatttt ttgagtgatc ttctcaaaaa atactacctg tcccttgctg atttttaaac
     4201 gagcacgaga gcaaaacccc cctttgctga ggtggcagag ggcaggtttt tttgtttctt
     4261 ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg ttttggtcgg cactgccgac
     4321 agcctcgcag agcacacact ttatgaatat aaagtatagt gtgttatact ttacttggaa
     4381 gtggttgccg gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta
     4441 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagctggact aaaaggcatg
     4501 caatttcata atcaaagaga gcgaaaaagt agaacgaatg atgatattga ccatgagcga
     4561 acacgtgaaa attatgattt gaaaaatgat aaaaatattg attacaacga acgtgtcaaa
     4621 gaaattattg aatcacaaaa aacaggtaca agaaaaacga ggaaagatgc tgttcttgta
     4681 aatgagttgc tagtaacatc tgaccgagat ttttttgagc aactggatcc aggagaacaa
     4741 aaacgatttt ttgaggaaag ttataaatta ttttccgaac gatatggcaa gcaaaatatt
     4801 gcttatgcaa cagttcataa tgatgagcaa acccctcaca tgcatttagg tgttgtgcct
     4861 atgcgtgatg gaaaactgca aggaaaaaat gtgtttaatc gtcaagaact gttatggcta
     4921 caagataaat tccccgagca tatgaaaaaa cagggttttg agttgaagcg tggtgaacgt
     4981 ggctctgacc gtaaacatat tgagacagct aaatttaaaa aacaaacttt ggaaaaagag
     5041 attgattttc tagaaaaaaa tttagcagtt aaaaaagatg aatggactgc ttatagcgat
     5101 aaagttaaat cagatttaga agtaccagcg aaacgacaca tgaaaagtgt tgaagtgcca
     5161 acgggtgaaa agtccatgtt tggtttggga aaagaaataa tgaaaacaga aaagaaacca
     5221 accaaaaatg ttgttatatc ggagcgtgat tataaaaact tagtgactgc tgcgagagat
     5281 aacgataggt taaaacagca tgttagaaat ctcatgagta ctgatatggc gagagaatat
     5341 aaaaaattaa gtaaagaaca tgggcaagtt aaagaaaaat atagtgtttt ttagagcgat
     5401 ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga aaacaagtct ttaaagtcta
     5461 aaataagcga tttaaagcgt gatgtgagtt taatctatga aagcactaag gaattcctta
     5521 aggaacgtac agacggctta aaagccttta aaaacgtttt taaggggttt gtagacaagg
     5581 taaaggataa aacagcacaa ttccaagaaa aacacgattt agaacctaaa aagaacgaat
     5641 ttgaactaac tcataaccga gaggtaaaaa aagaacgaag tcgagatcag ggaatgagtt
     5701 tataaaataa aaaaagcacc tgaaaaggtg tctttttttg atggttttga acttgttctt
     5761 tcttatcttg atatatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt
     5821 gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa
     5881 aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct
     5941 tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg
     6001 gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat
     6061 tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct
     6121 gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg
     6181 ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt
     6241 cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt
     6301 ct
//
LOCUS       DAFBYI010000294         5906 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.136, whole genome shotgun sequence.
ACCESSION   DAFBYI010000294 DAFBYI010000000
VERSION     DAFBYI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.136"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..2147
                     /locus_tag="LLD04_002909"
     CDS             <1..2147
                     /locus_tag="LLD04_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5649766.1"
                     /translation="YFGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNV
                     GGGMIGAILYHFTYFLIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQE
                     LLEGDPAKQARKQAAKEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENT
                     ADEWQEPENTEPEQLSFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDG
                     TSLEFEIEAEQENQDYELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAK
                     VVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEV
                     PNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGK
                     SVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQK
                     VVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVA
                     SNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRT
                     IIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKM
                     MPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEA
                     HGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            2377..3444
                     /gene="rlmN"
                     /locus_tag="LLD04_002910"
     CDS             2377..3444
                     /gene="rlmN"
                     /locus_tag="LLD04_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5649767.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3637..4740)
                     /locus_tag="LLD04_002911"
     CDS             complement(3637..4740)
                     /locus_tag="LLD04_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5649768.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5293..5841
                     /gene="aac(6')"
                     /locus_tag="LLD04_002912"
     CDS             5293..5841
                     /gene="aac(6')"
                     /locus_tag="LLD04_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5649769.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1966 a         1025 c         1275 g         1640 t
ORIGIN      
        1 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
       61 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
      121 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
      181 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
      241 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
      301 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
      361 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
      421 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
      481 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
      541 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
      601 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
      661 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
      721 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
      781 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
      841 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
      901 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
      961 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     1021 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     1081 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     1141 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     1201 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     1261 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     1321 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     1381 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     1441 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     1501 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     1561 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     1621 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     1681 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     1741 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     1801 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     1861 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
     1921 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
     1981 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
     2041 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
     2101 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
     2161 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
     2221 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
     2281 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
     2341 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
     2401 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
     2461 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
     2521 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
     2581 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
     2641 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
     2701 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
     2761 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
     2821 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
     2881 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
     2941 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
     3001 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
     3061 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
     3121 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
     3181 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
     3241 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
     3301 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
     3361 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
     3421 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
     3481 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
     3541 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
     3601 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
     3661 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
     3721 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
     3781 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
     3841 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
     3901 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
     3961 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
     4021 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
     4081 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
     4141 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
     4201 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
     4261 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
     4321 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
     4381 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
     4441 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
     4501 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
     4561 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
     4621 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
     4681 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
     4741 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
     4801 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
     4861 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
     4921 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
     4981 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
     5041 tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt
     5101 tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa
     5161 tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
     5221 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
     5281 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
     5341 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
     5401 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
     5461 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
     5521 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
     5581 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
     5641 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
     5701 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
     5761 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
     5821 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
     5881 taagaaggaa tttacgaaaa ttactt
//
LOCUS       DAFBYI010000295         4907 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.147, whole genome shotgun sequence.
ACCESSION   DAFBYI010000295 DAFBYI010000000
VERSION     DAFBYI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.147"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..374
                     /locus_tag="LLD04_002913"
     CDS             <1..374
                     /locus_tag="LLD04_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK5649770.1"
                     /translation="NRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPNSGQPVYRLY
                     NPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKTGTHHYTLSA
                     KERDWLTPMGWRYEGIGFYGY"
     gene            861..1325
                     /locus_tag="LLD04_002914"
     CDS             861..1325
                     /locus_tag="LLD04_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5649771.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            1309..1938
                     /locus_tag="LLD04_002915"
     CDS             1309..1938
                     /locus_tag="LLD04_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5649772.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            2292..3770
                     /gene="msr(C)"
                     /locus_tag="LLD04_002916"
     CDS             2292..3770
                     /gene="msr(C)"
                     /locus_tag="LLD04_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5649773.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3817..4263
                     /locus_tag="LLD04_002917"
     CDS             3817..4263
                     /locus_tag="LLD04_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5649774.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4462..>4907)
                     /locus_tag="LLD04_002918"
     CDS             complement(4462..>4907)
                     /locus_tag="LLD04_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5649775.1"
                     /translation="LITIFWEAHHSQIYTTLAKLDQQGYVTVLDKDEHSQKKIYHLTD
                     EGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQK
                     KLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT         1593 a          827 c         1008 g         1479 t
ORIGIN      
        1 ataaccgtca tacaggagaa catttatata cattgaatgc cggagaaaaa aattatctgc
       61 caactgtagg ctgggaatat gaagggattg cttggcaagc accaaacagc ggacaacctg
      121 tctaccgttt atacaatcct tacagtggag accatcatta cacaatggca caatctgaga
      181 tcaactcttt aacaaaaatc ggttggcgtt atgaaggtct aagtttttat tctggtggtt
      241 ccaagccaat ttatcgacta tttaatccaa atgaaaaaac tggtacccat cactatacct
      301 tgagtgctaa ggaaagagat tggctaacac cgatgggctg gagatatgaa ggaataggat
      361 tttatggtta ttaaaaagag gtagtgaaaa agctgccctg catcaagtag taagaacagc
      421 caaacaaaaa cagattttca tgttttttgt ttggttgaac ttattaccgc agatgcaagt
      481 ttttgaacac cgtttactca aaaaaggttg tgacaacatc ttgtcataac ttttttatta
      541 ctgtttaagc atactgatcc aatttctaat attcgacggg attcttgcaa aaggcgattt
      601 cggaaatttt tggcggtgta aaattttcta ctgcagaaga gctatcttct atagtttgat
      661 aagaatcaat aatcttggtt ttagcgttgt gctttcttta tcattttttc cgttcattct
      721 acaaaaaatt tcttttctct atacttttct attgagagcg ttttttttat tacacagctg
      781 attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa
      841 ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta tatcaaatca
      901 aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata agtcttacta
      961 gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc agtttgcaag
     1021 aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg
     1081 gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag
     1141 aaaaagccca gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc
     1201 tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga
     1261 atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa
     1321 aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac
     1381 aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct
     1441 gtcaatatgc aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg
     1501 cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt
     1561 tttggcgata tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca
     1621 gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag
     1681 agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg
     1741 caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggattcgaa
     1801 gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt
     1861 ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag
     1921 atcactacgt tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa
     1981 tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac
     2041 gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc
     2101 atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat
     2161 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
     2221 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
     2281 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
     2341 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
     2401 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
     2461 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
     2521 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
     2581 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
     2641 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
     2701 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
     2761 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
     2821 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
     2881 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
     2941 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
     3001 aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
     3061 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
     3121 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     3181 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     3241 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     3301 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     3361 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     3421 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     3481 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     3541 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     3601 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     3661 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     3721 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     3781 atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga
     3841 ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta
     3901 tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg
     3961 gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa
     4021 ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg cttgatggtt
     4081 ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg aatacctgat
     4141 caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg
     4201 cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca aagtaatgaa
     4261 tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa
     4321 tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt cggatcgctt
     4381 tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa tagatatttc
     4441 tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc ttacttgctg
     4501 acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa cataacgtcc
     4561 aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt tttgcatgat
     4621 tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt tatcgagcgt
     4681 cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg gagtttcctc
     4741 tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga tctttttttg
     4801 agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact tggcaagcgt
     4861 tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagct
//
LOCUS       DAFBYI010000296         4714 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.152, whole genome shotgun sequence.
ACCESSION   DAFBYI010000296 DAFBYI010000000
VERSION     DAFBYI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.152"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            177..1679
                     /gene="eat(A)"
                     /locus_tag="LLD04_002919"
     CDS             177..1679
                     /gene="eat(A)"
                     /locus_tag="LLD04_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5649776.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1707..2273
                     /locus_tag="LLD04_002920"
     CDS             1707..2273
                     /locus_tag="LLD04_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5649777.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2484..3545
                     /locus_tag="LLD04_002921"
     CDS             2484..3545
                     /locus_tag="LLD04_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5649778.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3548..4444
                     /gene="murQ"
                     /locus_tag="LLD04_002922"
     CDS             3548..4444
                     /gene="murQ"
                     /locus_tag="LLD04_002922"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5649779.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4458..>4714
                     /locus_tag="LLD04_002923"
     CDS             4458..>4714
                     /locus_tag="LLD04_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HBK5649780.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAG"
BASE COUNT         1638 a          802 c         1059 g         1215 t
ORIGIN      
        1 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
       61 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
      121 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
      181 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
      241 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
      301 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
      361 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
      421 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
      481 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
      541 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
      601 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
      661 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
      721 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
      781 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
      841 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
      901 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
      961 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     1021 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     1081 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     1141 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     1201 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     1261 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     1321 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     1381 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     1441 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     1501 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     1561 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     1621 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     1681 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     1741 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     1801 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     1861 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     1921 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     1981 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     2041 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     2101 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     2161 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     2221 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     2281 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta
     2341 ggttatgaca attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt
     2401 taataaaagt attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac
     2461 ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt
     2521 cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca
     2581 cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag
     2641 gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg
     2701 aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt
     2761 taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa
     2821 tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag
     2881 ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg
     2941 acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag
     3001 gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg
     3061 aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg
     3121 atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt
     3181 tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat
     3241 atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag
     3301 atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag
     3361 ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca
     3421 aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc
     3481 taccattgat caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa
     3541 gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac
     3601 ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca
     3661 gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac
     3721 gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag
     3781 actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt
     3841 cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga
     3901 ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt
     3961 tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa
     4021 atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca
     4081 cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt
     4141 gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg
     4201 tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat
     4261 cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa
     4321 gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag
     4381 tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt
     4441 atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa
     4501 tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg
     4561 tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag
     4621 gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga
     4681 acaaagtagc acagcaaatg gttgatttgg cagg
//
LOCUS       DAFBYI010000297         3485 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.179, whole genome shotgun sequence.
ACCESSION   DAFBYI010000297 DAFBYI010000000
VERSION     DAFBYI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.179"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            complement(<1..1166)
                     /locus_tag="LLD04_002924"
     CDS             complement(<1..1166)
                     /locus_tag="LLD04_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBK5649781.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQ"
     gene            1312..1887
                     /locus_tag="LLD04_002925"
     CDS             1312..1887
                     /locus_tag="LLD04_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5649782.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            2101..2796
                     /gene="vanR"
                     /locus_tag="LLD04_002926"
     CDS             2101..2796
                     /gene="vanR"
                     /locus_tag="LLD04_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5649783.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            2774..>3485
                     /locus_tag="LLD04_002927"
     CDS             2774..>3485
                     /locus_tag="LLD04_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5649784.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLV"
BASE COUNT         1146 a          623 c          669 g         1047 t
ORIGIN      
        1 ttttgcagta agtctaaata atcatagtcg gcaggacgtg caagttcctg agcctcttct
       61 actgaagaga caaaggtatt ccattcaata accgattcta aaaccttaaa aacgtctaat
      121 ttttcctctc ttgctttaat taatgcttgt ccgatgttcg taaagtgtat aactttctca
      181 tttagctttt taccgttttg tttctggatt tcctcttgag ccttacgacc ttttgataac
      241 aaactaagta tttgcctatc atgaatttca aacgctttat ccgttagctc ctgagtaagt
      301 tgtaataaat agatggttaa tatcgaataa cgtttatttt cttgaaagtc acggaatgca
      361 tacggctcgt atcttgagcc taagcgagac agctgcaaca ggcggttacg gtgcaaatga
      421 ctaatttgca ctgtttctaa atccattcct cgtatgtatt cgagtcgttc tattattttt
      481 agaaaagttt cgggtgaagg atgacccggt ggctctttta accaacccaa tatcgtttta
      541 ttggattcgg atggatgctg cgaggtaata atcccttcaa gcttttcttt ttgctcattt
      601 gttagagatt tactaaccgt attaaatagc ttcttttcag ccattgccct tgcttcccac
      661 accattcttt caagtgtagt gatagcaggc agtataattt tgttttttct tagaaaatct
      721 atgcattcat gcagtagatg aatggcatca ccattttcca aagctaattg atgaaggtac
      781 ttaaatgtca ttcgatattc actcagggta aaagttacaa agtcgtattc acttcgaatt
      841 tctttcaaat gatcccaaag tgtattttcc ctttgaggat aatgatcaag cgaggatgga
      901 ctaacaccaa tctgtttcga tatatattgt atgaccgaat ctgggatgct tttgatatga
      961 gtgtatggcc aaccgggata ccgaagaaca gctaattgaa cagcaaatcc taaacggttt
     1021 tcttccctcc ttcgcttatt aactatttct aaatcccgtt tggaaaaagt gaagtaggtc
     1081 cccagtatcc attcatcttc agggatttgc ataaaagcct gtctctgttc cggtgtaagc
     1141 aattctctac ctctcgcaat tttcattcag tatcattcca tttctgtatt ttcaatttat
     1201 tagttcaatt atatatcaat agagtgtact ctattgatac aaatgtagta gactgataaa
     1261 atcatagtta agagcgtctc ataagacttg tctcaaaaat gaggtgatat tttgcggaaa
     1321 atcggttata ttcgtgtcag ttcgactaac cagaatcctt caagacaatt tcagcagttg
     1381 aacgagatcg gaatggatat tatatatgaa gagaaagttt caggagcaac aaaggatcgc
     1441 gagcaacttc aaaaagtgtt agacgattta caggaagatg acatcattta tgttacagac
     1501 ttaactcgaa tcactcgtag tacacaagat ctatttgaat taatcgataa catacgagat
     1561 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
     1621 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
     1681 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
     1741 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
     1801 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
     1861 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
     1921 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
     1981 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
     2041 aattcggccc tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact
     2101 atgagcgata aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta
     2161 tacttaaaaa acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa
     2221 tgtatagaca agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc
     2281 ggccttacta tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc
     2341 gggaaagata cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata
     2401 acgaagccct ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac
     2461 aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc
     2521 attaatgtta acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc
     2581 gagttttcaa tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg
     2641 ctatttcatg agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg
     2701 catatccggc atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa
     2761 acggtatggg gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga
     2821 acgaaaactt tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta
     2881 tattcgttca atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa
     2941 atatgactta aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat
     3001 agatatcttt atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct
     3061 ttcaaaattc gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa
     3121 cgaagataaa caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac
     3181 attaaaacgg actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa
     3241 tgacgttgtt atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta
     3301 tttgagcctg cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca
     3361 tatcacgttg gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac
     3421 acggtataac ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct
     3481 ggtgc
//
LOCUS       DAFBYI010000298         2821 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.194, whole genome shotgun sequence.
ACCESSION   DAFBYI010000298 DAFBYI010000000
VERSION     DAFBYI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.194"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..764
                     /locus_tag="LLD04_002928"
     CDS             <1..764
                     /locus_tag="LLD04_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5649785.1"
                     /translation="AGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSPDSVADYTMM
                     LILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIERLRGFGCK
                     VLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQGAFLINT
                     GRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKLQRMPNV
                     IITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            757..1788
                     /gene="vanA"
                     /locus_tag="LLD04_002929"
     CDS             757..1788
                     /gene="vanA"
                     /locus_tag="LLD04_002929"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5649786.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEQEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            1794..2402
                     /locus_tag="LLD04_002930"
     CDS             1794..2402
                     /locus_tag="LLD04_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5649787.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          843 a          534 c          703 g          741 t
ORIGIN      
        1 gagccggtgt gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg
       61 ctgctaagag aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg
      121 attatactat gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg
      181 tggaaaaaca tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag
      241 ttggtgtggt gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg
      301 gatgtaaagt gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg
      361 atgagttgct gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc
      421 actatattat cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata
      481 ctgggcgcgg tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac
      541 tgggcggtgc cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca
      601 cccaaaaacc aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa
      661 tcacaccgca tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca
      721 ttaaaaactg tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata
      781 ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc
      841 gctaacatta ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta
      901 tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta
      961 ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc
     1021 aaccatgttg atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata
     1081 caaggtctgt ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca
     1141 atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc
     1201 gccttttggg ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt
     1261 tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac
     1321 gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag
     1381 gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagctgtt
     1441 ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag
     1501 caggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag
     1561 cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta
     1621 gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact
     1681 ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca
     1741 cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa
     1801 taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca
     1861 cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga
     1921 catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg
     1981 gattgcttct atgggacggt taccgtccta atcgtgctgt aaactgtttt atgcaatggg
     2041 ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg
     2101 agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg
     2161 atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt
     2221 ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc
     2281 gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt
     2341 ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat
     2401 aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga
     2461 cgtatgtaac tggttcttag ggaatttata tatagtagat agtattgaag atgtaaggca
     2521 gagcgatatt gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat aataagactg
     2581 atcgcataga ggggtggtat ttcacaccgc ccattgtcaa caggcagttc agcctcgtta
     2641 aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata gtaaatccag
     2701 tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca aacgagattg
     2761 tgccgtttaa ggggaagatt ctgggagcgt caataatttt gtgtaaataa ctcgtccttc
     2821 t
//
LOCUS       DAFBYI010000299         2821 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.195, whole genome shotgun sequence.
ACCESSION   DAFBYI010000299 DAFBYI010000000
VERSION     DAFBYI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.195"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..764
                     /locus_tag="LLD04_002931"
     CDS             <1..764
                     /locus_tag="LLD04_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5649788.1"
                     /translation="AGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSPDSVADYTMM
                     LILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIERLRGFGCK
                     VLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQGAFLINT
                     GRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKLQRMPNV
                     IITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            757..1788
                     /gene="vanA"
                     /locus_tag="LLD04_002932"
     CDS             757..1788
                     /gene="vanA"
                     /locus_tag="LLD04_002932"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5649789.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            1794..2402
                     /locus_tag="LLD04_002933"
     CDS             1794..2402
                     /locus_tag="LLD04_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5649790.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          842 a          535 c          703 g          741 t
ORIGIN      
        1 gagccggtgt gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg
       61 ctgctaagag aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg
      121 attatactat gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg
      181 tggaaaaaca tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag
      241 ttggtgtggt gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg
      301 gatgtaaagt gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg
      361 atgagttgct gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc
      421 actatattat cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata
      481 ctgggcgcgg tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac
      541 tgggcggtgc cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca
      601 cccaaaaacc aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa
      661 tcacaccgca tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca
      721 ttaaaaactg tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata
      781 ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc
      841 gctaacatta ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta
      901 tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta
      961 ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc
     1021 aaccatgttg atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata
     1081 caaggtctgt ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca
     1141 atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc
     1201 gccttttggg ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt
     1261 tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac
     1321 gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag
     1381 gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagctgtt
     1441 ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag
     1501 ccggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag
     1561 cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta
     1621 gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact
     1681 ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca
     1741 cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa
     1801 taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca
     1861 cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga
     1921 catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg
     1981 gattgcttct atgggacggt taccgtccta atcgtgctgt aaactgtttt atgcaatggg
     2041 ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg
     2101 agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg
     2161 atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt
     2221 ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc
     2281 gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt
     2341 ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat
     2401 aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga
     2461 cgtatgtaac tggttcttag ggaatttata tatagtagat agtattgaag atgtaaggca
     2521 gagcgatatt gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat aataagactg
     2581 atcgcataga ggggtggtat ttcacaccgc ccattgtcaa caggcagttc agcctcgtta
     2641 aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata gtaaatccag
     2701 tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca aacgagattg
     2761 tgccgtttaa ggggaagatt ctgggagcgt caataatttt gtgtaaataa ctcgtccttc
     2821 t
//
LOCUS       DAFBYI010000300         2218 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.211, whole genome shotgun sequence.
ACCESSION   DAFBYI010000300 DAFBYI010000000
VERSION     DAFBYI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.211"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..635
                     /locus_tag="LLD04_002934"
     CDS             <1..635
                     /locus_tag="LLD04_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK5649791.1"
                     /translation="GERSQHSSLMAAAIGTGLLWFGWFGFNSGGALRADEQAVNAFGS
                     TFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAAITPCAGYVEAKSAMLIGIIA
                     GIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIGIFASNSHLLTNPSSWYFLGI
                     QVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEEGLDFFFFGNKLN"
     gene            666..884
                     /locus_tag="LLD04_002935"
                     /pseudo
     CDS             666..884
                     /locus_tag="LLD04_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..1776
                     /gene="dfrF"
                     /locus_tag="LLD04_002936"
     CDS             1303..1776
                     /gene="dfrF"
                     /locus_tag="LLD04_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBK5649792.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1903..>2218
                     /locus_tag="LLD04_002937"
     CDS             1903..>2218
                     /locus_tag="LLD04_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBK5649793.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INS"
BASE COUNT          732 a          319 c          489 g          678 t
ORIGIN      
        1 taggagaaag aagtcagcat agcagtctta tggcagctgc tatcggtaca ggactgcttt
       61 ggtttggctg gttcggtttt aattccggtg gtgcacttcg agcagatgaa caagcagtca
      121 atgcatttgg ttcaacattc atcgctcttg catttgcgat gattacttgg ctgatcattg
      181 caaaagtaaa aggaaatggc tttgattttg tcgacgtact aacaggatcc gttgctggat
      241 tggcagccat tacgccttgt gccggttatg tagaagcaaa aagtgccatg ctgattggga
      301 tcattgcggg tatcgtctgt catgctgctg ttgattttcg taaaaagaaa caatgggatg
      361 atgcacttga tgtgtggggt gttcatggca tgggcggatt cacaggtacg attctgatag
      421 ggatctttgc atctaacagc catttactaa cgaatccaag cagctggtac ttcttaggga
      481 ttcaagtgat tggtgtcatc attactgccg tgtatgccta tgtgctgaca acagtgattt
      541 taaaatcggc gatgcacttt acaacgatca ctactacaaa agaagaacaa gaagaaggat
      601 tggacttttt cttctttggc aacaaactca attgaaacaa attcaaatag aaaagaggaa
      661 gattcatgca acaaagcaaa acgaaaattc ctgcaaaaaa attaacgttc tttggctttt
      721 tggctatgac catctcaatg gtcgtttcat tatatgaata tcccacattc gcgacctcag
      781 gcttcagctt agtatttttc ttgttattag gcggtttgct atggtttatc cctgtggcat
      841 tgtgcgcagc agagatggca acggtgaaag gatgggaaaa aggtgtttcc caaacaaaaa
      901 gatattaaga aacagcccat gtttacctgc ttgcaaacaa aagaatattg ctactagact
      961 aagagatact acataagtgt gttggtatct cttttttgtt gccgtgaaac agcagactat
     1021 ttcatttctg cttgattacc ttaaaactta ttagtctatg gtttgtcaag ggcttgttgc
     1081 gtgagaaatg ctgaatatca ccctttacaa aacataggct gataagtaac ttttcaaaag
     1141 cagaaaagaa atggcaatgc ttataacaat atgatataat gcagatgaag acaaaaaagg
     1201 aatttgtcga gctggttagt tcgagataat tttgagtttg ttaagatgtg gtgcacttga
     1261 tggaatcaca ggtacgacat catattatgg aggatgaaat agatgatagg tttgattgtt
     1321 gcgaggtcaa agaataatgt tataggcaag aatggtaata taccatggaa aataaaggga
     1381 gaacaaaagc aatttagaga gttaacaacg ggtaatgtgg ttattatggg gcgaaagtct
     1441 tatgaagaaa tcggtcatcc gttgcctaat agaatgaata ttgttgtttc caccacaaca
     1501 gagtatcaag gagataattt agtttcagtt aaatcattag aagatgcatt attattggct
     1561 aaaggacgag atgtatacat atctggtgga tatggactat ttaaggaagc tttgcaaata
     1621 gtagataaaa tgtatatcac agaagtagat ttaaatattg aagatggaga tacattcttt
     1681 ccagaatttg atatcaatga ttttgaagtt ttgatagggg aaacacttgg tgaggaagtg
     1741 aaatatacga gaacatttta tgtaaggaaa aaatgaattg agtaaatttt ggatttaggg
     1801 tgttttcata aatatattgc tttttatgca tatataaatt gttgtaagac aaattccagt
     1861 ttgtatgggg cgataaaatc agaatttgag agaggattga atatgaataa ggaatggtct
     1921 gaacttaata aaacaatgca ggcacaaata aaaaagaaag atacttataa gaggggtatt
     1981 gatactttac ttactttacg aagtcagtta atacaaacct tagtatcttt caaagaagaa
     2041 ttatgcagag aagattttaa ctcaatcccc ttcataaacg ctgatggtta tcatagtaag
     2101 acgattgctt attctatttg gcatattttc cgcattgaag atatagttgt gcatacagtg
     2161 ataaatgaag atgaacaagt attttttgca ggcaattatc aagagcgtat caattcac
//
LOCUS       DAFBYI010000301         2058 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.213, whole genome shotgun sequence.
ACCESSION   DAFBYI010000301 DAFBYI010000000
VERSION     DAFBYI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.213"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            94..1005
                     /locus_tag="LLD04_002938"
     CDS             94..1005
                     /locus_tag="LLD04_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK5649794.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1158..1643
                     /gene="vanZ"
                     /locus_tag="LLD04_002939"
     CDS             1158..1643
                     /gene="vanZ"
                     /locus_tag="LLD04_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5649795.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          721 a          293 c          381 g          663 t
ORIGIN      
        1 ttcctttttt ctatttacac aaaatatttt acactctcaa gattctagaa atatttcata
       61 cttccaacta tatagttaag gaggagactg aaaatgaaga agttgttttt tttattgtta
      121 ttgttattct taatatactt aggttatgac tacgttaatg aagcactgtt ttctcaggaa
      181 aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac atttagaaaa tagtgggact
      241 tctgaaaata cccaagagaa aacaattaca gaagaacagg tttatcaagg aaatctgcta
      301 ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga agtcagatat cgtgaattta
      361 tctaaacatg acgaattaat aaatggatac gggttgcttg atagtaatat ttatatgtca
      421 aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt
      481 cattttatta ttaatagtgg ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa
      541 atgggggctg agtatgcctt accagcaggt tatagtgagc ataattcagg tttatcacta
      601 gatgtaggat caagcttgac gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa
      661 aatgcttgga aatacgggtt cattttacgt tatccagagg acaaaacaga gttaacagga
      721 attcaatatg aaccatggca tattcgctat gttggtttac cacatagtgc gattatgaaa
      781 gaaaagaatt tcgttctcga ggaatatatg gattacctaa aagaagaaaa aaccatttct
      841 gttagtgtaa atggggaaaa atatgagatc ttttattatc ctgttactaa aaataccacc
      901 attcatgtgc cgactaatct tcgttatgag atatcaggaa acaatataga cggtgtaatt
      961 gtgacagtgt ttcccggatc aacacatact aattcaagga ggtaaggatg gcggaatgaa
     1021 accaacgaaa ttaatgaaca gcattattgt actagcactt ttggggtaac gttagctttt
     1081 taatttaaaa cccacgttaa ctaggacatt gctatactaa tgatacaact taaacaaaag
     1141 aattagagga aattatattg ggaaaaatat tatctagagg attgctagct ttatatttag
     1201 tgacactaat ctggttagtg ttattcaaat tacaatacaa tattttatca gtatttaatt
     1261 atcatcaaag aagtcttaac ttgactccat ttactgctac tgggaatttc agagagatga
     1321 tagataatgt tataatcttt attccatttg gcttgctttt gaatgtcaat tttaaagaaa
     1381 tcggattttt acctaagttt gcttttgtac tggttttaag tcttactttt gaaataattc
     1441 aatttatctt cgctattgga gcgacagaca taacagatgt aattacaaat actgttggag
     1501 gctttcttgg actgaaatta tatggtttaa gcaataagca tatgaatcaa aaaaaattag
     1561 acagagttat tatttttgta ggtatacttt tgctcgtatt attgctcgtt taccgtaccc
     1621 atttaagaat aaattacgtg taagatgtct aaatcaagca atctgatctt tcatacacat
     1681 aaagatattg aatgaattgg attagatgga aaacgggatg tggggaaact cgcccgtagg
     1741 tgtgaagtga ggggaaaacc ggtgataaag taaaaagctt acctaacact atagtaacaa
     1801 agaaagccca attatcaatt ttagtgctga ggaattggtc tctttaataa atttccttaa
     1861 cgttgtaaat ccgcattttc ctgacggtac ccccaaacta agatttcata gatagatttt
     1921 ctgataactt taaaaaacac gaatagcgga aaggatgtct tcattcgcta aagagtaata
     1981 aactactttt ccatcttgac gtgattttgc cattccatgt ttttttaaaa aacgtaaatg
     2041 gtgtgacgtt gtagcgac
//
LOCUS       DAFBYI010000302         1802 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.220, whole genome shotgun sequence.
ACCESSION   DAFBYI010000302 DAFBYI010000000
VERSION     DAFBYI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.220"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            245..1213
                     /locus_tag="LLD04_002940"
     CDS             245..1213
                     /locus_tag="LLD04_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5649796.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..>1802
                     /locus_tag="LLD04_002941"
     CDS             1206..>1802
                     /locus_tag="LLD04_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5649797.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQ"
BASE COUNT          544 a          341 c          424 g          493 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aa
//
LOCUS       DAFBYI010000303         1290 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-120
            SAMN22135363-rid13227983.guided.243, whole genome shotgun sequence.
ACCESSION   DAFBYI010000303 DAFBYI010000000
VERSION     DAFBYI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135363
            Sequence Read Archive: SRR16347119
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162964.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162964.1
            Genome Coverage       :: 30x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2021 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,943
            CDSs (total)                      :: 2,904
            Genes (coding)                    :: 2,832
            CDSs (with protein)               :: 2,832
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135363-rid13227983.guided.243"
                     /isolate="P-120"
                     /isolation_source="abscess, liver"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-08"
     gene            <1..105
                     /locus_tag="LLD04_002942"
     CDS             <1..105
                     /locus_tag="LLD04_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBK5649798.1"
                     /translation="ITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK"
     gene            83..1237
                     /locus_tag="LLD04_002943"
     CDS             83..1237
                     /locus_tag="LLD04_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5649799.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          421 a          241 c          286 g          342 t
ORIGIN      
        1 atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa
       61 tatataaaaa cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc
      121 caaactagaa cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt
      181 cgtgttgtat attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt
      241 ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg
      301 gaacaatata gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg
      361 cgtcatgctt tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact
      421 tattcagaac gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa
      481 gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca
      541 aagaaaaaat gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat
      601 tatcggttat ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa
      661 gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt
      721 tgagattaca cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata
      781 ctatatgctg gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca
      841 ggcggttatt cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag
      901 agtctttaac aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga
      961 cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat
     1021 cccaaaagat aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc
     1081 ttccgatacg ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca
     1141 tggagggcag atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc
     1201 agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa ttttggctct
     1261 ttgtcaaatg atgttggtaa agagaaatct
//
